U.S. patent number 3,843,045 [Application Number 05/351,364] was granted by the patent office on 1974-10-22 for centrifuge rotor.
This patent grant is currently assigned to Beckman Instruments, Inc.. Invention is credited to William L. Schmidt, Herschel E. Wright.
United States Patent |
3,843,045 |
Schmidt , et al. |
October 22, 1974 |
CENTRIFUGE ROTOR
Abstract
A rotor for a centrifuge apparatus having a substantially
cylindrical sidewall forming a bowl-like chamber open on one side
thereof, and including a cover secured to the cylindrical sidewall
and closing the opening to the chamber. The rotor also includes a
resilient sealing lip attached to the sidewall and overhanging the
chamber, the lip having an upper surface engaging a lower surface
of the cover and having sufficient resiliency to follow movement of
the cover under hydrostatic pressures produced in the rotor during
centrifugation of liquid products within the chamber.
Inventors: |
Schmidt; William L. (Boulder,
CO), Wright; Herschel E. (Santa Clara, CA) |
Assignee: |
Beckman Instruments, Inc.
(Fullerton, CA)
|
Family
ID: |
23380596 |
Appl.
No.: |
05/351,364 |
Filed: |
April 16, 1973 |
Current U.S.
Class: |
494/27; 494/79;
494/38 |
Current CPC
Class: |
B04B
7/08 (20130101); B04B 2007/025 (20130101) |
Current International
Class: |
B04B
7/08 (20060101); B04B 7/00 (20060101); B04b
007/06 () |
Field of
Search: |
;233/1R,26,32,33,44,27,28,46,1B,1A |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Krizmanich; George H.
Attorney, Agent or Firm: Steinmeyer; R. J. Mehlhoff; F.
L.
Claims
What is claimed is:
1. A rotor for a centrifuge apparatus comprising
a rotatable bowl having a base and a substantially cylindrical
sidewall forming a chamber having an open side substantially
coextensive with said cylindrical sidewall;
a cover for closing said open side of said chamber;
means for securing said cover to said sidewall of said rotatable
bowl; and
a resilient lip attached to said sidewall and overhanging said
chamber, said lip having an upper surface engaging the lower
surface of said cover, said lip having sufficient resiliency to
permit it to move and follow movement of said cover thereby
maintaining sealing contact with said cover during centrifugation
of liquid products within said chamber.
2. A rotor for a centrifuge apparatus comprising
a rotatable bowl having a base and a substantially cylindrical
sidewall forming a chamber having an open side substantially
coextensive with said cylindrical sidewall;
a cover for closing said open side of said chamber, including means
engaging said sidewall for supporting said cover over said chamber,
said cover having a substantially flat lower surface;
means for delivering liquid gradient and sample products through
said cover into said chamber; and
a resilient lip attached to said sidewall and overhanging said
chamber, said lip having an upper surface engaging said
substantially flat surface of said cover, said lip having
sufficient resiliency to permit it to move and follow movement of
said cover thereby maintaining sealing contact with said cover
during centrifugation of liquid products within said chamber.
3. The rotor defined in claim 2 in which said cover includes an
annular groove formed in the lower surface thereof in which is
mounted a resilient O-ring and said resilient lip extending from
said sidewall is so constructed and arranged as to engage said
O-ring and follow movement thereof to maintain sealing contact with
said O-ring during centrifugation of liquid products within said
chamber.
4. The rotor defined in claim 1 in which said resilient lip is
relatively thick at its attachment to said sidewall and gradually
reduces in thickness to form a resilient portion which moves in
response to hydrostatic pressure in said chamber created during
centrifugation thereby forcing said resilient lip to maintain
contact with said cover.
5. The rotor defined in claim 4 in which said cover is provided
with an annular groove in the lower surface facing said chamber,
and an O-ring is disposed in said groove and said resilient lip is
so positioned and disposed as to engage said O-ring thereby
maintaining sealing contact with said O-ring and said cover during
centrifugation of liquid products within said chamber.
Description
BACKGROUND OF THE INVENTION
The invention described herein relates generally to rotors for
centrifuge apparatus and more particularly to an improved bowl-type
rotor of the type employed for zonal centrifugation.
The apparatus is utilized to provide quantitative separations of
minute particulate matter, such as tissue cells or cell particles,
or the like, in density gradients for the purpose of collecting
discrete fractions thereof for subsequent analysis in research and
clinical studies. Typically, in a bowl-type rotor, a sample
solution is pumped into the central region of a rotor chamber which
is filled with an appropriate density gradient solution. During
centrifugation, the particles of interest in the sample disperse in
a radial direction throughout the density gradient solution and at
equilibrium are suspended in concentric layers in the density
gradient solution at a location wherein their respective buoyant
densities correspond to that of the solution.
During centrifugation of bowl-type rotors tremendous hydrostatic
pressures are developed within the rotor at the extremities or
outer walls of the bowl. The hydrostatic pressures tend to force
the wall of the rotor outwardly and also create an upward thrust on
the cover in the region adjacent the joint between the bowl rotor
and its cover. The outward pressure on the rotor walls also creates
a moment of force around the rotor bowl which, in turn, creates an
upward thrust at the central portion of the bowl. The hydrostatic
forces are so great that they sometimes cause separation between
the cover of the bowl and the wall of the rotor where connected and
result in a leakage of the liquid solution from the rotor. In an
attempt to overcome this, a resilient seal, such as a resilient
O-ring, is sometimes employed between the rotor wall and the cover
which will prevent leakage of the solution from the rotor chamber.
However, the seal member around the cover or the sidewall of the
rotor is placed under extreme stress during the high speed
centrifugation and, when the rotor and lid separate, the force of
the solution on the seal very often causes the seal member to
extrude from its seat thereby releasing the solution from the
chamber.
SUMMARY OF THE INVENTION
In accordance with the invention, there is provided an improved
bowl-type zonal centrifuge rotor including a base having a
substantially cylindrical sidewall forming a bowl-shaped chamber
over which is mounted a cover member and which is attached to the
cylindrical sidewall. A resilient flexible sealing lip protrudes
inwardly from the sidewall over the rotor chamber, the lip having a
smooth surface adapted to abut against the cover in the region of a
resilient sealing member or O-ring seated in an annular slot formed
in the cover. The flexible lip maintains a sealing pressure against
the cover and its O-ring and, under hydrostatic pressure created
during centrifugation, the flexible lip moves with any slight
movement of the cover to maintain a constant sealing pressure
against the O-ring. A central core positioned within the rotor
between the cover and the bottom of the chamber provides a vertical
thrust against the bottom wall of the rotor chamber which thrust is
opposite from the moment of force created by the centrifugal forces
on the sidewall of the rotor. This reduces the upward displacement
of the bottom wall of the rotor and reduces the movement of the
sidewall in a direction radial to the axis of rotation.
It is an object of the present invention to provide a new and
improved bowl-type rotor with an attached cover in which the
hydrostatic forces, created by the liquid within the rotor during
centrifugation, are reduced in the region of the seal between the
cover and the sidewall to which it is attached.
It is another object of this invention to reduce the deflection
between the cover and the sidewalls of the bowl by reducing the
hydraulic axial loading on the cover.
It is another object of the present invention to provide a
bowl-type rotor having a central core designed to oppose the upward
thrust normally occurring in the bottom central portion of the
bowl-shaped rotor during high speed centrifugation.
Further objects and advantages of the invention will become
apparent as the following description proceeds, and the features of
novelty which characterize the invention will be pointed out with
particularity in the claims annexed to and forming a part of the
specification.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a vertical sectional view of a rotor bowl assembly made
in accordance with the present invention;
FIG. 2 is a plan view taken generally along the line 2--2 with
portions of the rotor bowl assembly broken away to illustrate the
location of the central core and the core wings with respect to the
chamber in the rotor.
DESCRIPTION OF THE PREFERRED EMBODIMENT
Referring now to the drawing, and, more particularly, to FIG. 1,
there is shown a rotor bowl 1 having a substantially cylindrical
sidewall 4 enclosing a bowl-shaped chamber 3 over which there is
positioned a lid or cover member 2. In order to retain the cover 2
in place, the sidewall 4 of the rotor is provided with threads 5
adjacent the open end thereof and the outer edge portion 4a of the
rotor sidewall is adapted to fit snugly within an annular cavity 6
formed in the cover 2. Threads 7 on the cavity wall are adapted to
mate with the thread 5 on the rotor sidewall so that the cover may
be threaded down tightly onto the sidewall of the motor. The
overhanging edge 2a of the cover helps retain the sidewall 4 in
place against the centrifugal forces produced during high speed
rotation of the apparatus. In the base portion 1a of the rotor,
there is provided a well 8 which is adapted to receive a drive
spindle (not shown) extending from the driving mechanism of a
centrifuge apparatus.
Within the chamber 3, there is provided a rotor core or centerpiece
9 which is a relatively solid member, formed of a suitable metal,
and which is tapered slightly in an outward direction from bottom
to top. The core is provided with a plurality of slots 12 (see FIG.
2) formed in the outer surface thereof in a direction parallel to
the vertical axis of the core 9. A plurality of projecting vanes or
wings 11 (three of which are shown in FIG. 2) each have one end
positioned in one of the slots 12 and extend radially outward
thereby dividing the rotor chamber into equal sector compartments
for minimizing turbulence during operation of the apparatus.
Because the core or centerpiece 9 is subjected to high axial
loading and because the core wings 11 must extend to the cavity
wall under the lip 47, it is necessary to construct the core 9 and
vanes 11 as separate parts and assemble them in the rotor chamber
3.
In assembling the rotor core 9 within the chamber 3, the vanes 11
are first inserted into the camber 3 and positioned generally into
their relative positions therein. The core 9 is then inserted into
the chamber so that the ends of the vanes extend into slots 12
formed in the rotor core. In order to facilitate insertion of the
core or centerpiece 9 and permit orientation of the vanes 11 within
the slots 12, each of the slots 12 is indented in the lower end
adjacent the bottom of the core and tapered or beveled to form a
ramp 10 so that there is a slight tolerance, permitting the ends of
the vanes to fit initially into the slots 12. As the core
centerpiece 9 is moved downwardly into the chamber, the ramp 10
forces the vanes 11 outwardly against the inner surface of the
sidewall of the motor chamber. The outer ends of the vanes 11
conform to the bowl shape of the chamber and fit snugly against the
sidewall beneath the lip 47. The rotor core abuts the bottom 32 of
the cavity and legs or abutments 31 support the core thereon.
Inserted centrally within the rotor core is a feed or transfer tube
assembly 13 which extends downwardly through an aperture 14 formed
in the cover 2 into a cavity 16 formed in the rotor core 9. The
lower end of the transfer tube 13 is provided with screw threads 17
which mate with internally formed threads 18 formed within the
cavity 16 of the rotor core. The transfer tube 13 includes an outer
conduit 19 (or plurality of conduits 19) formed as slots in the
outer surface of the shaft 13. Slots 19 communicate with an
enlarged opening 16a in the upper portion of the rotor core. This
opening 16a in turn communicates with radial passages 22 in the
wall of the core each of which communicate with passages 23
extending through each of the vanes or wings 11 and opening into
the chamber 3 at the end of each wing. An opening 24, formed in a
beveled or cutaway portion 26 of each vane, communicates with the
upper portion of the chamber. The core member acts as a manifold to
deliver fluids into each of the vanes 11.
The feed or transfer tube 13 is also provided with a central
tubular conduit 27 which extends completely through the axial
length of the transfer tube and communicates with a passage 28
formed in the lower end of the core. Passages 29 formed in the
lower end of the rotor core 9 by means of abutments or shoulders 31
communicate with the passage 28 in the core. At least one of the
passages 29 leads into each of the segments between the vanes 11
within the chamber. Attached to the upper end of the transfer tube
is a cup 34 having an opening 35 therein which communicates with
the passages 19 and 27 in the transfer tube. In operation, a liquid
delivery means (not shown) is inserted into the opening of the cup
34 for introducing liquid gradient and sample materials through the
passages 19 and 27 and thence into the rotor chamber. Cup 34 is
firmly attached to the end of the transfer tube 13 and is tightly
pulled downwardly against the upper surface 36 of the lid 2 so that
an O-ring 37 formed within an annular slot in the lower surface of
the cup abuts in sealing fashion against the upper surface 36 of
the lid.
In order to provide a seal between the passages 23 formed in the
vanes 11 and the passages 22 formed in the rotor centerpiece, a
small O-ring or resilient seal ring 41 is provided within an
enlarged opening surrounding the pasage 23. O-ring 41 seals around
the surface of the pasage 23 and the surface of the core leading
into the opening 22. Another resilient seal member 42 is fitted
within a slot formed on the surface of the transfer tube 13 and
abuts against the inner surface of the cavity 21 in the upper end
16a of the core centerpiece. This seal 42 prevents the flow of
liquid beyond the upper end 16a of the cavity 21 and forces liquid
from the cavity into the passages 22 and thence into the passages
23 formed in vanes 11. In order to prevent leakage around the upper
surface of the core centerpiece 9, there is also provided an
annular sealing means in the form of a resilient O-ring 43
positioned within an annular slot 44 in the upper surface of the
core. The O-ring 43 abuts against the lower surface 46 of the cover
2 when the cover is tightened down onto the rotor. In addition the
cover actually has its lower surface 46 abutting tightly against
the upper flat surfaces of the core.
In a typical operation of the above-described rotor, a rotatable
liquid delivery means (not shown) is inserted into the cup 34
through which a liquid may be pumped into the rotor. The rotor bowl
is first loaded with a liquid gradient which is pumped into the
bowl through the conduits 19, 21, 22 and 23. During the loading
operation, the rotor bowl is rotated at a low speed (500-3,000
r.p.m.) causing the gradient liquid to form concentric annular
"zones" of constant density liquid. The vanes 11 extend radially
through the "zones," segmenting them and reducing mixing between
adjacent zones of different densities. After the rotor bowl is
filled with a liquid of graded density, a sample to be centrifuged
is pumped through the conduit or transfer tube passage 27 where it
flows through the passage 28 and the passages 29 beneath the rotor
core into the center portion of the rotor chamber. The sample is
normally followed by an overlay of low density liquid to move the
sample clear of the core 9 and to assure complete removal of the
sample from beneath the rotor core. The means for inserting the
liquid materials into the rotor is then removed and the cap 34 is
closed with a suitable sealing cap (not shown) to close the
passages 19 and 27. The rotor is then accelerated for the actual
centrifuging operation.
When centifugation has been completed, the rotor bowl is
decelerated to unloading speed (500-3,000 r.p.m.) and the rotor
contents emptied through the liquid delivery means by pumping high
density fluid to the outer wall of the rotor cavity through the
passages 19, 21 22 and passages 23. The high density fluid forces
the gradient and the sample, dispersed in the annular gradient
rings, toward the rotor core 9 and the taper on the exteral surface
of the rotor core forces each of the gradient rings to flow
downwardly through the passages 29 and up the central passage 27 of
the transfer tube and thence out through the delivery means
attached to the upper end of the transfer tube.
As the rotor is subjected to the high angular velocity by a drive
hub at 8, the resulting hydraulic forces within the chamber cause
the cover 2 and the bowl 1 to tend to move relative to one another.
These members are restrained only by the threads and the
overhanging lip 2a of the cover at 2a. This hydraulic force is
proportional to the fourth power of the maximum radius at which it
can act. In order to effectively reduce the radius at which these
forces may act on the cover, the rotor is provided with a lip 47
extending from an upper portion of the sidewall 4, which intrudes
radially into the clearance volume adjacent the lid 2 of the rotor.
The lip 47 has a lower curved surface 47a and an upper flat surface
47b. At its base or connection with sidewall 4, the lip is
relatively thick but it gradually tapers toward the end intruding
into the chamber.
The thinner portion of the lip 47 are somewhat resilient and flex
under the influence of axial hydraulic force such that as the lid 2
moves upwardly under the hydraulic pressure, the lip 47 moves just
enough to follow the cover's motion and maintains contact with a
resilient O-ring seal 48 positioned in an annular slot 49 within
the lower surface of the cover. The upper surface 47b of the lip 47
is flat and maintains continuous contact with the O-ring 48 as the
cover and sidewalls of the motor move relative to each other. At
the base of the lip 47, it is much thicker and much less resilient
so that any load exerted by the hydraulic forces is prevented from
being transmitted to the cover in this region. The hydraulic forces
may be transmitted in an upward direction toward the cover only at
the inner edge of the resilient lip 47. Since this is at a radius
much less than the outer extremity of the rotor wall, the hydraulic
pressure exerted against the inner surface of the cover 2 is less
than would normally be present if there were no resilient lip or
seal abutting the lower surface of the cover.
Under the influence of centrifugal force, the wall of the rotor at
51 tends to move radially outward. Since this wall is attached to
the base and restrained by the base, this tendency to move outward
results in a moment about the center of the base causing the center
to move upwardly relative to the threads of the cylindrical wall at
7. This upward movement of the central portion of the base is
restrained by virtue of the force exerted by the cover 2 downwardly
against the heavy metallic core member 9 which tends to exert a
force axially against the base. By restraining the center of the
base from bulging upward by the use of the rigid central core 9,
the radial excursion of the sidewall 4 of the rotor is reduced with
a corresponding reduction in stresses in the sidewall 4 under high
speed centrifugation.
While in accordance with the patent statutes there has been
described what at present is considered to be the preferred
embodiment of the invention, it will be understood by those skilled
in the art that various changes and modifications may be made
therein without departing from the invention, and it is, therefore,
the aim of the appended claims to cover all such changes and
modifications as fall within the true spirit and scope of the
invention.
* * * * *