U.S. patent number 3,811,842 [Application Number 05/260,551] was granted by the patent office on 1974-05-21 for temperature-controlled fluid manifold for a fluid system of an automated sample analyzer.
This patent grant is currently assigned to Technicon Instruments Corporation. Invention is credited to Herman G. Diebler, Steven Andrew Gyori, William J. C. McCandless.
United States Patent |
3,811,842 |
Diebler , et al. |
May 21, 1974 |
TEMPERATURE-CONTROLLED FLUID MANIFOLD FOR A FLUID SYSTEM OF AN
AUTOMATED SAMPLE ANALYZER
Abstract
Temperature-controlled fluid manifold for a fluid system of an
automated sample analyzer of the type for analyzing a series of
liquid samples flowing seriatum to quantitative analysis for a
constituent of interest. The basic elements of the
temperature-controlled manifold may be permanently combined with a
wide variety of other components to meet the requirements of many
different chemistries to provide many different
temperature-controlled manifolds each suited for analysis of a
different constituent of a sample such as blood for example. In
each particular manifold, the sample may be treated as by
combination and mixing with any appropriate reagent or reagents
under temperature-controlled conditions for subsequent analysis as
in a colorimeter for example. The manifold may be significantly
smaller in size and less costly than conventional manifolds. The
aforementioned basic manifold elements comprise a thermally
conductive plate or block heated by conduction from a
temperature-controlled source of heat, which plate has an outer,
exposed surface of substantial area, and an appropriate number of
appropriately configured fluid passageway portions, such as helical
mixing or helical time-delay paths, encapsulated in a solid
material characterized by a heat storage capacity and which
material is supported in an appropriate location on the
last-mentioned surface for thermal transfer by conduction to the
material from the plate. The manifold includes a cover effectively
tending to stagnate the ambient atmosphere around such encapsulated
fluid passageway portions.
Inventors: |
Diebler; Herman G. (North
Haledon, NJ), Gyori; Steven Andrew (Allendale, NJ),
McCandless; William J. C. (Ringwood, NJ) |
Assignee: |
Technicon Instruments
Corporation (Tarrytown, NY)
|
Family
ID: |
22989622 |
Appl.
No.: |
05/260,551 |
Filed: |
June 7, 1972 |
Current U.S.
Class: |
422/81; 422/82;
422/109; 165/236 |
Current CPC
Class: |
B01L
7/00 (20130101); G01N 35/08 (20130101) |
Current International
Class: |
B01L
7/00 (20060101); G01N 35/08 (20060101); G01n
033/16 () |
Field of
Search: |
;23/230,253,292,259
;126/263 ;137/334 ;165/18 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Serwin; R. E.
Attorney, Agent or Firm: Tedesco; S. P. Rockwell; S. E.
Claims
1. In a manifold for an automated fluid analyzer, having at least
one temperature-controlled fluid passageway portion, the
combination of: a support, a heated plate, having thermal transfer
characteristics, supported on such support and having an exposed
outer surface area, and a fluid passageway portion encapsulated in
a block of solid material having thermal storage characteristics,
providing access to an inlet and an outlet in said passageway
portion, and located in a selected one of a plurality of locations
on said surface area, said block of encapsulation material having a
substantial surface area opposed to and secured in close proximity
to said heated plate for thermal transfer by conduction from said
plate to said block of material, means to regulate the heating of
said plate, and a cover extending with clearance over said block
encapsulating said fluid passageway portion, effectively tending to
stagnate the ambient atmosphere about the external exposed surface
of said block of encapsulation material, thereby tending to prevent
thermal
2. A manifold as defined in claim 1, wherein: a plurality of
different ones of such encapsulated fluid passageway portions are
secured to and in close proximity to said heated plate for thermal
transfer thereto from said
3. A manifold as defined in claim 1, wherein: said fluid passageway
portion
4. A manifold as defined in claim 1, wherein: said fluid passageway
portion is of generally duplex spiral configuration with a fluid
admission port for the admixture of a fluid to the spiral
passageway between the two
5. A manifold as defined in claim 1, further including a fluid
analyzer supported from said support and in fluid flow
communication with said
6. A manifold as defined in claim 1, further including at least two
fluid analyzers supported from said support in fluid communication
with each
7. A manifold as defined in claim 1, wherein: said cover extends
over the
8. A manifold as defined in claim 1, further including heating
means for
9. A manifold as defined in claim 1, wherein: said fluid passageway
portion is formed of an inert material and the last-mentioned
material is
10. A manifold is defined in claim 2, wherein: the manifold has a
plurality of fluid inlet and outlet conduits external thereto and
extending within said cover.
Description
BACKGROUND OF THE INVENTION
1. Field of the Invention
This invention relates to a temperature-controlled fluid manifold
for a fluid system of an automated sample analyzer of the type for
analyzing a series of liquid samples flowing seriatum to
quantitative analysis for a constituent of interest.
2. Prior Art
Apparatus for the continuous analysis of fluids are well known.
Such an apparatus is disclosed in Skeggs U.S. Pat. No. 2,797,149
issued June 25, 1957. Skeggs U.S. Pat. No. 2,879,141 issued Mar.
24, 1959 discloses analysis apparatus of an automated type in which
samples are fed in a flowing stream by means of a takeoff device
which aspirates liquid from each of a plurality of sample
containers which are sequentially presented thereto by a sampler
assembly. Such apparatus is commonly employed for the analysis of
various fluids. Skeggs et al. U.S. Pat. No. 3,241,432 issued Mar.
22, 1966 discloses automated apparatus for performing multiple
quantitative analyses on different portions of a single sample of a
series of such samples, each analysis being for a different
specific sample constituent. As shown and described in the
last-mentioned patent by way of example, such automated analysis
apparatus may include for analysis purposes a series of different
analytical manifolds for different tests, the flow from each one of
which is to analysis by a colorimeter or a spectral flame
photometer for analysis of different portions of a same sample.
However, other conventional photometric analysis devices or
ion-selective electrodes, for example, may be used for analysis
purposes in similar automated sample analysis apparatus.
The aforementioned Skeggs U.S. Pat. No. 2,797,149 which discloses
an automated analyzer which is essentially a one-channel or a
one-manifold system, and in the system illustrated in FIG. 3 of the
drawings there is a water bath, indicated at 64, to control the
temperature of a treated sample portion flowing through the bath in
a helical coil. The water in the water bath 64 is heated by an
immersion heater of the rod type, and the temperature of the water
bath 64 is suitably regulated. The water bath 64 is of considerable
size and may be supported on a table surface. It is also relatively
expensive. Another similar water bath, indicated at 99, is shown in
a different system described in that patent and illustrated in FIG.
4. Aforementioned Skeggs U.S. Pat. No. 2,879,141 discloses in FIG.
8 of the drawings similar fluid baths for the temperature control
of a treated sample portion flowing therethrough.
Aforementioned Skeggs et al. U.S. Pat. No. 3,241,432 discloses as
in FIG. 1 of the drawings heating baths 141 and 142 associated with
respective ones of a pair of different analytical manifolds to
conduct different tests on different portions of a same sample
which is one of a series of such samples. The heating bath contains
oil which is heated as by a heater for heating the oil in each
instance and the treated sample portion is flowed through the
respective bath in a helical passageway which is encapsulated by
the oil. The last-mentioned heating baths are relatively large and
costly though they may be smaller and less costly than the heating
baths described above with reference to the other aformentioned
Skeggs patents.
A disadvantage of the aforementioned heating baths of the prior art
aside from possible heating fluid leakage, resides in the fact
that, at best, it would be difficult to miniaturize such baths to
the size desirable for use in today's automated sample analyzer
wherein more tests are performed on a single sample, that is where
the sample of a series undergoing analysis is divided into many
more aliquots, each of which receives a different test in a
different manifold for a constituent of interest in each sample.
Even if possible to miniaturize such conventional heating baths as
described above to desirable dimensional ranges for dimensionally
small manifolds, such water or oil bath heaters would be relatively
costly. Furthermore, plural heating baths have had individual
temperature-control elements, one for each heating bath. It has
been found in the use of such plural heating baths that there is a
lack of temperature uniformity between such heating baths.
SUMMARY OF THE INVENTION
One object of the invention is to provide an improved
temperature-controlled fluid manifold for a fluid system of an
automated sample analyzer of the type for analyzing a series of
liquid samples flowing seriatum to quantitative analysis for a
constituent of interest. Another object is to provide in such
temperature-controlled manifold, basic elements of the manifold
which may be permanently combined with a wide variety of other
components to meet the requirements of many different chemistries
to provide many different temperature-controlled manifolds each
suited for analysis of a different constituent of the sample such
as blood for example. Still another object is to provide, in such a
manifold, for treatment of the respective sample portion as by
combination and mixing with any appropriate reagent or reagents
under temperature-controlled conditions for subsequent analysis as
in a colorimeter for example.
Another object is to provide a manifold having plural
temperature-controlled fluid passage portions all under the control
of a single temperature regulator, effecting better uniformity of
temperature-control to the extent that the temperature of one such
fluid passage portion effectively tends to be the same as the
temperature of the other such fluid passageway portions.
Still another object is to provide a manifold which may be
significantly smaller in size and less costly than conventional
manifolds, and which is very durable and less subject to breakage
of parts than conventional manifolds.
There is provided a manifold, such as characterized above, the
basic elements of which comprise a thermally conductive plate or
block heated by conduction from a temperature-controlled source of
heat, which plate has an outer, exposed surface of substantial
area, and an appropriate number of appropriately configured fluid
passageway portions, such as helical mixing or helical time-delay
coils, encapsulated in a solid material characterized by a heat
storage capacity and which material is supported in a selected one
of a plurality of available locations on the last-mentioned surface
for thermal transfer by conduction to the material from the plate.
The manifold includes a cover effectively tending to stagnate the
ambient atmosphere around such encapsulated fluid passageway
portions.
BRIEF DESCRIPTION OF THE DRAWINGS
In the drawings:
FIG. 1 is a front elevational view of a temperature-controlled
fluid manifold, with the cover removed, embodying the
invention;
FIG. 2 is a bottom view of the manifold of FIG. 1; and
FIG. 3 is a sectional view taken along line 3--3 of FIG. 1.
DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS
With reference to the drawings, particularly FIGS. 2 and 3 thereof,
the manifold is shown as comprising an oblong base 10 preferably
constructed of material having thermal insulating properties, which
may be of a plastic material. The base 10 has a longitudinal,
horizontally extending opening therethrough. As shown in FIGS. 1
and 2 the outer part of the base 10 is cut-away in part as at 12 to
receive a heater block or plate 14 having an outer, exposed surface
16 of substantial area and which is substantially flush with the
right-hand end portion of the base 10 as viewed in FIG. 1.
Secured to the underside of the block or plate 14 in the location
indicated in FIGS. 1 and 2 is a thermostatically controlled heater
element 18 preferably in direct contact with the last-mentioned
side of the plate 14 for the transfer to the latter of thermal
energy from the heater element 18. The heater element 18 is
preferably of the electrical type, and is located within the
aforementioned horizontally extending opening through the base 10.
The heater plate 14 is characterized, at least to some extent, in
that the material from which it is formed has good thermal
conductivity characteristics. The material of the plate 14 is
preferably a metal and may be formed of powered aluminum or
stainless steel, for example.
In a vertical plane intermediate the plate 14 and the inner
extremity of the base 10 the base may be provided with an upwardly
directed shoulder 20 to act as a stop and an abutment for the
manifold cover 22 to limit inward movement of the last-mentioned
cover with reference to the base 10. The rigid cover 22 snugly fits
over the base 10 and if desired may be removed from the manifold on
outward sliding movement of the cover 22. It is to be understood
that the cover may be supported from the manifold in any suitable
manner as by being hinged thereto (not shown) and any suitable stop
may be employed to limit closing movement of the cover 22. The
cover is preferably transparent so that, when the cover is
assembled with the manifold base, a user may view through the cover
at least certain fluid stream portions in the use of the manifold,
as will appear more fully hereinafter.
As best shown in FIG. 1 one or more temperature-controlled fluid
passageway portions are associated with the heater plate 14 outside
the plate 14, two such temperature-controlled portions being
illustrated by way of example and indicated at 24 and 26.
Each such temperature-controlled fluid passageway portion 24, 26
defines a fluid passageway therethrough which is encapsulated in a
solid material having heat conductive and storage characteristics
which material may be formed of a metal such as lead or aluminum
for example. The passageway portion 24 is formed as a helix from
end-to-end with an inlet at one end thereof extending through an
encapsulation material and an outlet at the other end thereof, also
extending through the encapsulation material as shown in FIG.
1.
The temperature-controlled fluid passageway portion 26 includes two
helical portions in axial arrangement with reference to one another
and with communication therebetween, that is between the outlet of
the first helical portion and the inlet of the second helical
portion. The inlet of the firstmentioned helical portion of the
fluid passageway portion 26 extends through the encapsulation
material as shown in the last-mentioned view and the outlet of the
second mentioned helical portion of the fluid passageway portions
26 extends through the encapsulation material as also shown in this
view.
It has been found advantageous to form the aforementioned fluid
passageway portions 24 and 26 in a glass material and then
encapsulate the glass material in the aforementioned heat storage
material. One of the reasons for this is that glass of certain
well-known types is very inert and not subject to corrosive attack.
From the foregoing it will be understood that it is not usually
desirable to form the fluid passageway portion 24 and 26 directly
in the encapsulating material having heat storage properties. The
encapsulating material is in intimate contact with the glass
material, completely surrounding the helical portions of the glass.
In addition, the encapsulation material fills the axial area (FIG.
3) around which the turns of glass coil are formed. It has been
found that if the encapsulation material used is lead, a
temperature-controlled fluid passageway portion such as the portion
24 may be encapsulated by molding the preformed glass helix of the
portion 24 within the heat storage material. Of couse in such a
molding operation access must be provided to the inlet and the
outlet of the portion 24.
The encapsulated fluid passageway portions 24 and 26 are secured to
the outer surface 16 of the heater plate 14 in any suitable manner
as by cementing the units to the plate 14 in the desired location
on the outer surface 16 of the heater plate. The manner of securing
the encapsulated temperaturecontrolled fluid portions 24 and 26 to
the heater plate surface 16 is not deemed critical, but it should
be understood that the units 24 and 26 must be in proximity to the
surface 16 of the heater plate to receive thermal conduction
therefrom. It will also be noted in FIG. 1 that the electrical
heater element 18 is preferably located substantially centrally of
the area occupied by the units 24, 26 in plane laterally inwardly
of the plane of the heater plate 14, for substantially uniform heat
distribution of both units 24, 26.
The number and configuration of the temperature-controlled fluid
passageway portions will depend upon the particular chemical and
other requirements of the particular intended use of the manifold.
The illustrated manifold is for the quantitative determination of
SGPT in a blood sample. Accordingly the fluid connections to the
manifold and any temperature-controlled fluid passageway portions
must be suitable to the particular test. As seen in the lower
lefthand corner of FIG. 1 there is provided a fitting 28 which may
be of glass and constructed in accordance with the technique of
fabrication illustrated and described in the co-pending U.S. Pat.
application of De Angelis et al., Ser. No. 246,966 filed on Apr.
24, 1972, now U.S. Pat. No. 3,770,405. This fitting 28 does not
require detailed explanation here. It is sufficient for present
purposes that the fitting 28, which is suitably secured to the
surface 16 of the heater plate, has a passageway formed therein in
communication with flexible tubes 30, 32, 34 all of which are
inlets into the internal passageway in the fitting 28. The
last-mentioned passageway has an outlet in communication with the
inlet of glass tube 36. The material of which these tubes are
formed is not critical but for the sake of convenience they may be
flexible and formed of a suitable inert material. Tube 36 has an
outlet coupled to the inlet of temperature-controlled unit 26.
Downstream a short distance from the last-mentioned inlet, another
inlet in temperature-controlled unit 26 is provided in
communication with the outlet of tube 38 which may be formed of
inert glass and which is for the purpose of introducing another
fluid into the temperature-control unit 26.
Miscible fluids flowing into the temperature-control unit 26
through the tubes 36 and 38 are mixed within the first section of
the double helix of the unit 26. Prior to the introduction of fluid
from the last-mentioned section of the double helix to the second
section of the double helix of unit 26, another miscible fluid is
added to the temperature control unit 26 through a plastic tube 40
having an outlet coupled to a port in the section of the
temperature-control unit 26 providing communication between the two
helical sections thereof, so that the combined stream is passed
through the second or right-hand helical portion of the unit 26 for
mixing of the miscible fluid therein.
The fluid flowing into the inlet of tube 32 is immiscible with the
liquid flowing into the inlet of tube 30 and such immiscible fluid
may be an inert gas which segments the stream flowing from the
outlet of tube 30 in the aforementioned passage in fitting 28.
Another liquid immiscible with the liquid inletted from the tube 30
is inletted from the tube 34 into the last-mentioned passage in the
fitting 28 to combine with a stream in the fitting 28. The
segmented liquid stream outletted from the fitting 28 to the tube
36 is combined with the sample inletted into the stream through the
outlet of tube 38. The sample flowing into tube 38 is itself in
segmented condition while flowing in the tube 38. The inlets of the
tubes 30, 32, 34, 38 and 40 are not shown but it is to be
understood that they may be some distance away from the manifold
and have any type of suitable connection to the sources of the
respective fluids, for flow through the manifold induced in a
conventional manner by a pressure differential. As indicated in
FIGS. 1 and 2, the tubes 34 and 40 enter the manifold by passing
through respective holes in the base 10 beyond the right-hand
extremity of the heater plate 14 as seen in FIG. 1.
The tubes 30, 32 and 38 may enter the manifold through a common
opening 42 (FIG. 2) in the cover 22 which opening is sufficiently
small to essentially create a stagnant ambient atmosphere within
the cover 22 so that the temperature-controlled units 24 and 26 are
not subject to drafts when the cover 22 is in place, and changes in
the temperature of the ambient atmosphere within the cover 22 are
effectively inhibited when room temperature changes.
As previously indicated, all temperature-controlled fluid
passageway portions are under the common control of a single heat
regulator, and there is a large degree of uniformity of the
temperature of one temperature-controlled fluid passageway portion
with the temperature of all the other such fluid passageway
portions. The temperature may be in the order of 37.degree.C.
While the temperature-controlled fluid passageway portions 24 and
26 illustrated and described herein are of the type wherein a
sample is treated as by mixing or passing through an incubator,
fluid temperature-controlled passageway portions of other types and
within the purview of the invention may be utilized such as a
non-illustrated temperature-controlled flow controller for a
reagent fluid, associated in a similar manner with the heated plate
14, which flow controller may be secured to the face of the plate
14 obverse to that on which the temperature-controlled fluid
passageway portions 24 and 26 are secured.
The manifold may be mounted in the attitude of FIG. 1 on a panel of
which a large number of other manifolds are also mounted in an
analytical chemistry module of an automated fluid sample
analyzer.
The flow from the exit of the temperature-controlled unit 26 is
through glass tube 44 having an inlet end coupled to the fluid
outlet end of the temperature-controlled unit 26 and having an
outlet end coupled to an inlet conduit 46 in a flow cell 48. The
flow cell 48 may be of any conventional construction and is secured
in a conventional manner to the heater plate 14 though it may be
spaced outwardly from the surface 16 thereof. The flow cell 48 has
a fluid passageway portion 50 to one end of which the outlet of the
inlet 46 is connected, which portion 50 is arranged longitudinally
of an optical path. The fluid outlet from the portion 50 is to the
flow cell outlet 52. Beyond the inlet 46 and the outlet 52 and
within the passage portion 50, there are provided transparent fluid
seals, not shown, which lie in the optical path.
Optical fiber assemblies 54, 56 of elongated form and of
conventional construction each have an end aligned and supported
with reference to the respective ends of fluid passageway 50, lying
within the optical path, and which ends of the assemblies 54 and 56
are disposed outwardly of the respective last-mentioned fluid seals
of the passage 50. The optical fiber assembly 54 enters the
manifold through an opening (not shown) in the base 10 and passes
with clearance through the heater plate 14. The optical fiber
assembly 56 exits from the manifold through the base 10 and is
likewise provided with clearance with the heater plate 14.
Utilizing the optical fiber assemblies 54 and 56, the stream in the
passageway 50 of the flow cell 48 is analyzed colorimetrically at a
location remote from the manifold, and the signal from the
colorimeter (not shown) is suitably processed and may be displayed
in typical, non-illustrated fashion.
The outlet 52 of the flow cell 48 is coupled to the inlet end of a
glass tube 58 for the flow through the tube 58 of the stream
exiting from flow cell 48. The outlet end of the tube 58 is coupled
to the inlet end of temperature-controlled fluid passageway unit 24
and the stream flowing through the helix of the illustrated unit 24
under temperature-controlled conditions is incubated therein.
A glass tube 60 has an inlet end coupled to the fluid outlet of the
temperature-controlled fluid passageway portion 24 and has an
outlet end connected in similar fashion to a flow cell 62 similar
to the flow cell 48 and having optical fiber assemblies 64 and 66
similar to the optical fiber assemblies 54 and 56 associated
therewith and in a similar manner. These optical fiber assemblies
64 and 66 are utilized in a colorimetric analysis of the fluid
stream passing through the flow cell 62 in a similar manner. While
the fluid outlet of the flow cell 62 is shown as coupled to the
inlet end of a plastic waste tube 68 exiting from the manifold
through a hole, not shown, in the base 10 (FIGS. 1 and 2) for
simplicity of illustration of the invention, in practice the flow
from the flow cell 62 is directed through another
temperature-controlled unit (not shown) similar to the unit 24 and
is subsequently passed to still another flow cell (not shown), and
it is from the last-mentioned flow cell that the stream flows to
waste through the manifold in the manner of the exit tube 68
passing through the base 10. It is to be understood that the
non-illustrated additional temperature-controlled fluid passage and
the non-illustrated flow cell which receives the stream therefrom
are supported on the heater plate 14 in similar fashion and are
similarly configured.
The illustrated manifold may be approximately 121/2 inches long and
21/2 inches wide and may take the form of a cartridge suitably
secured through the base 10 to a vertical panel of a multiple
analyzer on which panel a relatively large number of such
cartridges for different chemistries or analyses may be mounted in
close proximity to one another. It will be understood that the
manifold of the invention may utilize other analysis means than
those illustrated. For example, the analysis may be by
potentiometric measurement.
While the invention has been illustrated with reference to a
preferred form and several embodiments have been discussed, it will
be apparent, especially to those versed in the art, that the
invention may take other forms and is susceptible of various
changes in details without departing from the principles of the
invention.
* * * * *