U.S. patent number 3,732,734 [Application Number 05/256,992] was granted by the patent office on 1973-05-15 for micropipette with disposable tips.
This patent grant is currently assigned to Centaur Chemical Co.. Invention is credited to Souren Avakian.
United States Patent |
3,732,734 |
Avakian |
May 15, 1973 |
MICROPIPETTE WITH DISPOSABLE TIPS
Abstract
A spring loaded plunger actuated vacuum pipette tube is
disclosed for use in combination with removable disposable molded
plastic tip members wherein a plurality of adjacent concentric
internal annular rings are formed within the larger open end of the
tip member, and annular rings being of successively decreasing
diameter progressing inwardly, for cooperative airtight frictional
engagement with a smoothly tapered end surface of the pipette tube.
The foregoing abstract is not to be taken either as a complete
exposition or as a limitation of the present invention. In order to
understand the full nature and extent of the technical disclosure
of this application, reference must be had to the following
detailed description and the accompanying drawing as well as to the
claims.
Inventors: |
Avakian; Souren (Westport,
CT) |
Assignee: |
Centaur Chemical Co. (Danbury,
CT)
|
Family
ID: |
22974449 |
Appl.
No.: |
05/256,992 |
Filed: |
May 25, 1972 |
Current U.S.
Class: |
73/864.14;
285/332; 141/311R; 422/931; 285/239 |
Current CPC
Class: |
B01L
3/0275 (20130101) |
Current International
Class: |
B01L
3/02 (20060101); B01l 003/02 () |
Field of
Search: |
;73/425.4P,425.6
;141/18,31,311 ;285/238,239,240,260,8 ;23/292 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Swisher; S. Clement
Claims
Having described my invention, what I claim as new and desire to
secure by Letters Patent is:
1. The combination of a spring loaded plunger actuated vacuum
pipette and a removable disposable plastic tip member comprising a
taper formed on the end of the pipette opposite the plunger
thereof, and a plurality of adjacent concentric internal ringed
ribs of successively decreasing diameter formed on the inner
surface of an open end of said plastic tip member, whereby said
ringed ribs frictionally engage said tapered end to form an
airproof seal therebetween.
2. The combination of claim 1 wherein the taper formed on the end
of said pipette is less than the taper formed between the inner
surfaces of the innermost and outermost of said successive internal
ringed ribs.
3. The combination of claim 1 and an outer annular shoulder portion
formed on the outer open end of said tip member adjacent to and
surrounding said concentric ringed ribs.
4. For use with a spring loaded plunger actuated vacuum pipette
instrument, a detachable and disposable tip member formed of molded
plastic in a substantial right cylinder elongated hollow cone,
having an outer annular ring portion formed around its larger open
end and at least one elastically deformable annular inner ringed
rib formed within said larger open end and concentric with the axis
of said cone.
5. The combination of claim 4 including a plurality of adjacent
annular ringed ribs formed on the inner conical surface of said tip
member within the larger open end thereof, said annular ringed ribs
being of progressively decreasing diameter inwardly from said open
end.
6. The combination of claim 5 wherein the diameters of said
adjacent annular ringed ribs decreases progressively in such manner
that a conical surface tangential to the inner surfaces of all said
annular ribs forms a greater taper than that of a pipette
instrument adapted for insertion therein.
Description
BACKGROUND OF THE INVENTION
In busy chemical and pharmaceutical laboratories, for the
expeditious performance of numerous analytical tests it is
necessary to quickly and accurately measure and deliver micro
volumes of various reagents and samples for analysis. For this
purpose various vacuum pipettes have been developed in the prior
art.
The present invention is directed to improved disposable pipette
tips, formed of injection molded plastic material, such as
polyethylene, which are functional for a wide range of applications
and are impervious to acids, alkalies and most organic solvents,
and have non-wetting characteristics. The tips are easily and
quickly inserted on the end of a pipette tube with which they form
an airtight seal, and are just as easily and quickly removable
therefrom for disposal after use. Because of their low cost the
plastic tips of the invention may be discarded after each use, thus
eliminating the need for washing and sterilization for the next
operation. By reason of an external annular shoulder formed around
the larger open end of each tip a quantity of tips may be stored in
a sanitary jig or fixture from which individual tips may be easily
picked up by simply inserting the end of a pipette tube, and they
may be subsequently removed from the instrument simply by pressing
the external shoulder against the lip of a disposal container --
all in a single handed operation.
OBJECTS OF THE INVENTION
It is an object of the invention to provide an inexpensive
disposable micropipette capable of easy and convenient one-hand
operation without need for or risk of the operator's hand touching
any part which comes in contact with chemical reagents.
It is a further object to provide such a pipette which is
impervious to acids, alkalies and organic solvents.
An overall object of the invention is to provide such a
micropipette which affords maximum convenience, cleanliness, ease
of operation and the elimination of cross-contamination.
Other objects of the invention will in part be obvious from the
following description and the claims.
The invention accordingly comprises an article of manufacture
possessing the features, properties, and the relation of elements
which will be exemplified in the article hereinafter described, and
the scope of the invention will be indicated in the claims.
BRIEF DESCRIPTION OF THE DRAWING
For a fuller understanding of the nature and objects of the
invention, reference should be had to the following detailed
description taken in connection with the accompanying drawing, in
which:
FIG. 1 is a horizontal view, partially cut away, of a pipette
instrument with a disposable tip member mounted on the vacuum end
thereof;
FIG. 2 is an enlarged view of the tip member mounted on the
instrument end, and shown partially in section substantially along
the line 2--2 of FIG. 1;
FIG. 3 is a cross-sectional view of the large end of the tip member
taken along the line 3--3 of FIG. 2; and
FIG. 4 is a greatly enlarged cross-sectional view of the large end
portion of the tip member of the invention showing the manner in
which the concentric internal annular ringed rib portions decrease
in diameter inwardly to provide effective airtight sealing means
for the pipette tube.
DETAILED DESCRIPTION
Referring now in greater detail to FIG. 1 of the drawing, a
preferred embodiment of the invention includes a vacuum pipette
instrument indicated generally at 5, having (from right to left) a
finger operated plunger 6 axially mounted within an annular finger
grip 7 which is affixed to one end of a hollow cylindrical tube 8.
Affixed to and coaxial with the opposite end of cylinder 8 is a
reduced diameter elongated hollow nozzle portion 9, the end of
which is tapered as may be seen more clearly in FIG. 2.
Still referring to FIG. 1, within the hollow cylindrical tube 8 is
a spring loaded air pump piston 10 which is operable by depression
and release of plunger 6 to produce a controlled stroke, measured
volume air displacement. An airtight axial valve formed between the
internal central rod 11 and a valve seat formed in coaxial bushing
12 is opened when the plunger 6 is depressed and is closed when
plunger 6 is fully released. As shown to the left in FIG. 1 an
elongated hollow tapered tip member 14, which is preferably formed
as a right circular cone, has its larger open end fitted over the
tapered end of nozzle 9 on the plunger instrument 5. The opposite
end 16 of tip member 14 is provided with a small diameter coaxial
opening through which controlled amounts of fluid may be admitted
and discharged when the tip end 16 is immersed in sample liquids or
reagents and the plunger 6 is depressed. In use the device of FIG.
1, including the pump instrument 5 with tip member 14 affixed as
shown, is held in a generally vertical position by one hand, with
two fingers under the annular grip 7 and a thumb or forefinger
resting on plunger 6 in a position for depressing the plunger at
will. The plunger 6 is then depressed, the tip 16 is immersed in
the liquid to be sampled, and the plunger 6 is then released,
thereby drawing an accurately measured volume of liquid into the
lower end of tip member 14. Because of the closure of the airtight
valve 11-12 upon release of plunger 6 the liquid drawn into a tip
member 14 remains therein, under the force of external atmospheric
pressure, until such time as the plunger 6 is again depressed to
expel the sample fluid from the small end opening at 16. Because
the total internal volume of the conical tip member 14 greatly
exceeds the accurately measured volumetric displacement of plunger
10 within cylinder 8, the sample fluid within the tip 14 never
rises far enough to contaminate (or be contaminated by) the nozzle
9 of instrument 5. After discharge of the measured sample from tip
14, the tip member may be easily removed from the nozzle 9 by a
simple "wiping" motion, as achieved by placing the annular shoulder
portion 17 against the inner lip of an open refuse container and
merely pulling backward (from left to right in FIG. 1) on the
annular finger grip 7. A fresh unused tip may then be easily picked
up simply by inserting the tapered end of nozzle 9 into the open
end of a new tip member, which may be conveniently held either by
hand or in a table top fixture rack.
The salient feature which facilitates the airtight engagement of
the tip members such as 14 with the instrument nozzle 9, and the
ease of application and removal therefrom as described above, will
now be described in detail with reference to FIGS. 2 through 4 of
the drawing.
Referring first to FIG. 2, which is an enlarged view of the
left-hand portion of FIG. 1 with the tip member 14 foreshortened at
13 to conserve drawing space and the right half of member 14 cut
away in section to show the internal cavity 20, the tapered end
surface 18 of nozzle 9 may be seen inserted into frictional
engagement with a plurality of inner annular ring ribbed portions
19 formed on the inner open end 15 of tip member 14. As the tip
member 15 is formed of injection molded plastic material, such as
polyethylene, the annular ribs 19 are semiflexible and compressible
and thus provide a mechanically rigid airtight seal with the
tapered metallic surface 18 of nozzle 9.
FIG. 3, being a cross-sectional view taken along the line 3--3 of
FIG. 2, further illustrates the cooperative engagement of collar
end 15 of tip member 14 surrounding the tapered surface 18 of
hollow nozzle 9.
Referring now to FIG. 4, which is a greatly enlarged
cross-sectional view of the right-hand end portion of tip member 14
as shown in FIG. 2, the plurality of inner annular ringed ribs
indicated generally as 19 in FIG. 2 are shown separately as 21
through 25. From this figure it may be seen that the annular ribs
21-25 decrease in diameter inwardly from 25 to 21; i.e., the inner
diameter of 25 is greater than that of 24, which in turn is greater
than 23, etc. Significantly, a line drawn tangent to the inner
annular surfaces of ribbed rings 21-25 forms a greater angle with
the horizontal (in FIG. 4) than does the taper 18 of nozzle 9 in
FIG. 2. Thus, when the tapered end of nozzle 9 is inserted into the
larger open end of tip member 14, the surface 18 first engages
outer annular rib 25; as the nozzle 9 is protruded further into tip
14 the surface 18 successively becomes frictionally engaged with
annular ribs 24, 23, etc., compressing each rib further as it is
inserted farther, thereby establishing both a mechanical coupling
and an airtight seal between tip member 14 and instrument 5.
It will thus be seen that the objects set forth above, among those
made apparent from the preceding description, are efficiently
attained and, since certain changes may be made in the above
article without departing from the scope of the invention, it is
intended that all matter contained in the above description or
shown in the accompanying drawing shall be interpreted as
illustrative and not in a limiting sense.
It is also to be understood that the following claims are intended
to cover all of the generic and specific features of the invention
which, as a matter of language, might be said to fall
therebetween.
* * * * *