Virus purification

Abstract

Described herein are processes for purifying infectious virus particles and uses of protamine in such processes.

Description

RELATED APPLICATION

This application is a national stage filing under 35 U.S.C. .sctn. 371 of International Patent Application Serial No. PCT/EP2016/082663, filed Dec. 23, 2016, the contents of which is incorporated herein by reference in its entirety.

FIELD OF THE INVENTION

The disclosure relates to methods for the purification of viruses for use in vaccines.

BACKGROUND OF THE INVENTION

Regulatory agencies such as the World Health Organization establish standards and guidelines for the production of pharmaceutical compositions administered to humans, such as vaccines, that limit quantity and components of the compositions. Meeting these standards is particularly challenging with regard to production of vaccines containing biological agents, such as viruses, which must be propagated on cell substrates. Such vaccine preparations must be sterile (i.e., free from independently replicating organisms) and may contain no more than 10 ng of host cell DNA per human dose, among other requirements. These standards are in place in order to ensure safety of the composition for human administration, but may introduce challenges in the development of processes used to produce such compositions.

Protamine was originally isolated from the sperm of salmon and other species of fish but is now produced primarily through recombinant biotechnology. It is a highly cationic peptide that binds to negatively charged molecules such as nucleic acids to form a stable ion pair. Its use in removing host cell nucleic acid is well document.

SUMMARY

During the course of routine virus purification, it was observed that addition of protamine sulfate to a virus harvest produced on a cell substrate removed not only contaminating DNA derived from host cells, as expected, but surprisingly also virtually eliminated immature and otherwise non-infectious virus particles from the preparation. This finding provides a streamlined, gentle, reproducible and broadly-applicable process for obtaining highly-purified infectious virus particles for applications such as vaccine preparation; furthermore, the process is not dependent on the charge of the virus particle.

Disclosed herein are downstream processes for purifying virus particles from a crude preparation. The downstream process can be applied to either a virus which has not adapted for propagation on a particular cell substrate or for a partial/fully cell substrate adapted virus particle.

Aspects of the invention provide processes for the purification of infectious virus particles comprising the steps of (a) providing a crude harvest (a) comprising virus particles and impurities, wherein the impurities are generated from growing said virus particles on a cell substrate; (b) reducing impurities from the crude harvest (a) by precipitation with an agent comprising a protamine salt, preferably a protamine sulphate, to obtain a virus preparation (b); and further purifying the virus preparation (b) by method or methods selecting for size of the virus particles, such as e.g. a sucrose density gradient centrifugation to obtain a virus preparation (c) comprising the infectious virus particles.

In some embodiments, the concentration of protamine sulphate in step (b) is about 1 to 10 mg/ml, more preferably about 1 to 5 mg/ml, more preferably about 1 to 2 mg/ml. In one embodiment, the concentration of protamine sulphate in step (b) is about 2 mg/mL. In one embodiment, the concentration of protamine sulphate is 1.2 to 1.8 mg/ml, more preferably 1.4 to 1.6 mg/ml. In a preferred embodiment, the concentration of protamine sulphate in step (b) is about 1.6 mg/ml or about 2 mg/ml.

In some embodiments, the residual host cell DNA of the virus preparation (e) is less than 1 .mu.g/mL, especially less than 900, 800, 700, 600, 500, 400, 300 or 200 ng/mL, preferably less than 100 ng/mL. In a preferred embodiment, the residual host cell DNA of the virus preparation (c) is less than 10 ng/mL. In some embodiments, the residual host cell protein of the final virus preparation (c) is less than 10 .mu.g/mL, especially less than 9, 8, 7, 6, 5, 4, 3 or 2 .mu.g/mL, preferably less than 1 .mu.g/mL. In a preferred embodiment, the residual host cell protein of the virus preparation (c) is less than 100 ng/mL. In some embodiments, the residual non-infectious virus particles of the final virus preparation (c) is less than 10 g/mL, especially less than 9, 8, 7, 6, 5, 4, 3 or 2 .mu.g/mL, preferably less than 1 .mu.g/mL. In a preferred embodiment, the residual non-infectious virus particles of the virus preparation (c) is less than 100 ng/mL.

In some embodiments, the residual protamine is less than 1 .mu.g/mL, especially less than 900, 800, 700, 600, 500, 400, 300 or 200 ng/mL, preferably less than 100 ng/mL, more preferably is below the detection limit of HPLC, in particular below the detection limit in the final drug substance. In some embodiments, the PS content is tested by HPLC or size exclusion chromatography (SEC). For example, HPLC is validated for PS determination in JEV sucrose gradient pool samples as a routine release assay and is very sensitive (i.e., LOQ 3 .mu.g/mL; LOD 1 .mu.g/mL). In the current invention, PS content in virus DS samples was <LOD. In one embodiment, the HPLC assessment of PS content can be performed on a Superdex Peptide 10/300GL column (GE: 17-5176-01) using 30% Acetonitrile, 0.1% Trifluoroacetic acid as solvent with a flow rate of 0.6 ml/min at 25.degree. C. and detection at 214 nm. A more sensitive method of measurement for residual protamine in a purified virus preparation is mass spectrometry (MS). In some embodiments, the residual PS levels in a virus preparation are tested by MS or other such highly sensitive method, e.g., nuclear magnetic resonance (NMR). With this method, residual PS, as well as fragments and/or break-down products of PS, can be detected at trace amounts, such as levels as low as, for example, 10.sup.6, 10.sup.7 or 10.sup.8 molecules per typical sample load. In some embodiments, the PS levels are tested in the sucrose gradient pool. In some embodiments, the PS levels are tested in the drug product. In some embodiments, the PS levels are tested in the drug substance.

In some embodiments, the crude harvest (a) comprising the virus particles and impurities is subjected to one or more pre-purification step(s) prior to step (b). In some embodiments, the one or more pre-purification step(s) comprises digesting host cell genomic DNA in the crude harvest (a) comprising the virus particles and impurities by enzymatic treatment. In some embodiments, the one or more pre-purification step(s) comprises filtration, ultrafiltration, concentration, buffer exchange and/or diafiltration. In some embodiments, the one or more pre-purification steps is filtration using a filter having a pore size equal to or less than 1 .mu.m. In some embodiments, the filter has a pore size equal to or less than 0.2 .mu.m. In a preferred embodiment, the filter has a pore size of 0.2 .mu.m. In some embodiments, the concentration and/or ultra/diafiltration and/or buffer exchange is performed by tangential flow filtration (TFF). In some embodiments, ultra/diafiltration of the crude harvest (a) comprising the virus particles and impurities is performed using a hollow fiber membrane having a cut-off of equal to or less than 300 kDa. In a preferred embodiment, the hollow fiber membrane has a cut-off of 100 kDa.

In some embodiments, the virus particle is a live virus, a chimeric virus, an attenuated live virus, a modified live virus, or a recombinant live virus. In a further step, the virus particles of the invention may by optionally inactivated. In some embodiments, the virus particle is an attenuated form of the virus particle. For example, the virus may have reduced infectivity, virulence, and/or replication in a host, as compared to a wild-type virus. In some embodiments, the virus is a mutated or modified virus, for example the nucleic acid of the virus may contain at least one mutation relative to the wild-type virus. In some embodiments, the virus is a recombinant live virus, meaning a virus that is generated recombinantly and may contain nucleic acid from different sources.

In some embodiments, the virus particle is a live virus, an attenuated live virus, a modified live virus, or a recombinant live virus. In some embodiments, the virus belongs to a virus family selected from the group consisting of Paramyxoviridae, Orthomyxoviridae, Flaviviridae, Filoviridae, Arenaviridae, Rhabdoviridae, and Coronaviridae. In some embodiments, the virus belongs to a virus family selected from the group consisting of Togaviridae (being live or inactivated), such as alphaviruses, or Flaviviridae (being live or inactivated). In some embodiments, the virus is a virus of the family Flaviviridae, i.e. a flavivirus. In other embodiments, the virus is a Zika virus or Yellow Fever virus. In preferred embodiments, the virus is a Zika virus. In a most preferred embodiment, the Zika virus is a Zika virus from the Asian lineage.

In some embodiments, the relative reduction of impurity of the final virus preparation relative to the liquid medium (a) comprising the virus particles and impurities is in a range from 60 to 95%. In some embodiments, the residual impurity of the final virus preparation is less than 1%. We observed a decrease in the HCP peaks and the non-infectious aggregate peaks in the HPLC-SEC or SDS-PAGE. An exact quantification is difficult but one can measure the density of the SDS-PAGE bands and other methods.

In some embodiments, the filtration of step in (b)(ii) of claim 1 is performed using a filter having a pore size equal to or greater than 1 .mu.m. In some embodiments, the filter has a pore size equal to or greater than 0.2 .mu.m. In a preferred embodiment, the filter has a pore size of 0.2 .mu.m.

In some embodiments, the virus is propagated in a cell line selected from the group consisting of an EB66 cell line, a Vero cell line, a Vero-.alpha.His cell line, a HeLa cell line, a HeLa-S3 cell line, a 293 cell line, a PC12 cell line, a CHO cell line, a 3T3 cell line, a PerC6 cell line, a MDSK cell line, a chicken embryonic fibroblast cell line, a duck cell line, and a diploid avian cell line. In some embodiments, said cell line is a duck cell line. In some embodiments, said cell line is a diploid avian cell line. In some embodiments, said cell line is EB66 cell line. In a preferred embodiment, said cell line is a Vero cell line.

Aspects of the invention provide a use of any of the processes described herein for manufacturing a composition for immunization against a viral infection. In a preferred embodiment, the composition is a vaccine. In one embodiment, the composition or vaccine is directed against Chikungunya virus. In one embodiment, the composition or vaccine is directed against a flavivirus. In one embodiment, the composition or vaccine is directed against Yellow Fever virus. In one embodiment, the composition or vaccine is directed against Zika virus such as e.g. a Zika virus of the Asian lineage.

Other aspects provide compositions comprising the virus particles obtainable by any of the processes described herein for treating and/or preventing a viral infection. In one embodiment, the viral infection is caused by Chikungunya virus. In one embodiment, the viral infection is caused by a flavivirus. In one embodiment, the viral infection is caused by Yellow Fever virus. In one embodiment, the viral infection is caused by Zika virus such as e.g. a Zika virus of the Asian lineage.

In some embodiments, the attenuated form of ChikV is derived from the LR2006-OPY1 ChikV infectious clone (La Reunion isolate). In some embodiments, the attenuated form of ChikV is the .DELTA.5nsP3 mutant as described by Hallengird et al. (Novel Attenuated Chikungunya Vaccine Candidates Elicit Protective Immunity in C57BL/6 mice (2014) Journal of Virology 88(5):2858-2866) or an immunogenic variant thereof. The immunogenic variant of the .DELTA.5nsP3 ChikV mutant is herein defined as having at least 80% sequence identity to the nucleotide sequence of the .DELTA.5nsP3 mutant sequence as provided by SEQ ID NO: 77, especially at least 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88% 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 77.

In some embodiments, the Zika virus is derived from the Asian lineage. In some embodiments, the Zika virus is a Zika virus as described partially or fully in Sequence section of this application, i.e. any of sequences SEQ ID Nos 2 to 69 or 78, in particular all partly or fully described Zika viruses of the Asian lineages or an immunogenic variant thereof. The immunogenic variants of the Zika virus or Zika virus of the Asian lineages are herein defined as having at least 80% sequence identity to the nucleotide sequence of the sequences described in any of sequences SEQ ID Nos 2 to 69 or 78, especially at least 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88% 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or at least 99% sequence identity.

In some embodiments, the process of the invention results in an enrichment of infectious virus particles from the crude harvest comprising infectious virus particles and non-infectious virus particles and other virus products such that the enrichment of the infectious virus particles is at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, preferably at least 80%, especially 85% relative to the total virus particle content of the crude harvest (a) comprising the virus particles and impurities.

In some embodiments, the residual impurity of the final virus preparation with respect to all impurities in the crude harvest is less than 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, preferably less than 5% as determined by SEC-HPLC (Size Exclusion Chromatography--HPLC).

In some embodiments, the filtration step of the virus preparation (b) after contact with the solid-phase matrix is performed using a filter having a pore size equal to or greater than 1 Lm. In some embodiments, the filter has a pore size equal to or greater than 0.2 Lm. In a preferred embodiment, the filter has a pore size of about 0.2 .mu.m, such as 0.22 .mu.m.

In some embodiments, the Zika virus, or Chikungunya virus is propagated in a cell line selected from the group consisting of an EB66 cell line, a Vero cell line, a Vero-.alpha.His cell line, a HeLa cell line, a HeLa-S3 cell line, a 293 cell line, a PC12 cell line, a CHO cell line, a 3T3 cell line, a PerC6 cell line, a MDSK cell line, a chicken embryonic fibroblast cell line, a duck cell line, and a diploid avian cell line. In some embodiments, said cell line is a duck cell line. In some embodiments, said cell line is a diploid avian cell line. In some embodiments, said cell line is EB66 cell line. In a preferred embodiment, said cell line is a Vero cell line.

Aspects of the invention provide a use of any of the processes described herein for manufacturing a composition for immunization against a Zika virus, Yellow Fever, or Chikungunya virus infection. In a preferred embodiment, the composition is a vaccine. In preferred embodiments, the vaccine is administered to the subject once, twice or three or more times. In a preferred embodiment, the vaccine is administered once or twice. In a preferred embodiment, the vaccine is administered only once.

The herein disclosed in vivo data regarding immunogenicity of the inactivated Zika virus vaccine of the current invention indicates that the virus is surprisingly potently immunogenic and also highly cross-protective (very similar immunogenicity in African and Asian strains). Data indicate that immunogenicity was unexpectedly higher than the recently reported inactivated Zika virus vaccine candidate (Larocca, et. al, 2016, Nature doi:10.1038/nature18952.). Inactivated viruses are among the safest vaccines and especially preferred for delivery to populations where safety is especially concerning, such as pregnant women, children and immunocompromised individuals, which makes the herein disclosed inactivated Zika virus particularly suitable. Obtaining a high titer of inactivated virus is a challenge in the field. The herein disclosed process for purifying inactivated Zika virus results in not only a high yield, but also a very pure drug substance.

Other aspects provide compositions comprising the virus particles obtainable by any of the processes described herein for treating and/or preventing a Chikungunya virus infection.

BRIEF DESCRIPTION OF THE DRAWINGS

The accompanying drawings are not intended to be drawn to scale. The figures are illustrative only and are not required for enablement of the disclosure. For purposes of clarity, not every component may be labeled in every drawing, alignments were performed with the multi alignment package Jalview (Waterhouse et al., 2009, Bioinformatics 25 (9) 1189-1191). In the drawings:

FIG. 1: Average distance tree (by % identity, nt), complete genomes.

FIG. 2: Neighbor joining tree (by % identity, nt), complete genomes.

FIG. 3: Pairwise alignment-Jalview (% identity, nt), complete genomes.

FIG. 4: Average distance tree (by % identity, aa), E-protein.

FIG. 5: Neighbor joining tree (by % identity. aa), E-protein.

FIG. 6: Pairwise alignment-Jalview (% identity, aa), E-protein.

FIG. 7: Alignment (shading: % identity, aa), E-protein.

FIG. 8: An example of virus particle maturation in the host cell. As observed in flaviviruses, full maturation of the particles requires proteolytic cleavage of the precursor membrane glycoprotein (prM) by the host protease furin. Not all prM molecules are cleaved, resulting in the release of mature, mosaic or immature-like conformations from the cells. Mosaic and immature forms are generally not infectious-only mature virions are infective and have hemagglutinin (HA)/TCID50 activity. (Figure adapted from Plevka, et al., Maturation of flaviviruses starts from one or more icosahedrally independent nucleation centres, EMBO reports (2011) 12, 602-606).

FIG. 9: CHIKV schematic genome, including non-structural and structural proteins (labeled "CHIKV") as well as a schematic representation of the .DELTA.5nsP3 attenuated Chikungunya virus used to exemplify the purification process of the current invention (labeled ".DELTA.5nsP3"). The black triangle indicates the approximate location of the deletion in the nsP3 coding region. (Figure adapted from Hallengird et al. 2014, supra.)

FIG. 10: Flow-chart showing an exemplary downstream .DELTA.5nsP3 CHIK virus purification process from the crude harvest to formulation of the (vaccine) drug substance, a preferred embodiment of the process of the invention.

FIG. 11: Absorbance at 214 nm, 260 nm and 280 nm of individual sucrose gradient centrifugation (SGC) fractions of a representative purification run of the process of the invention (A); the SEC-HPLC analysis of the final pooled fractions containing purified infectious attenuated .DELTA.5nsP3 ChikV virus particles (B); and a silver-stained SDS-PAGE gel showing the protein content of the virus preparation following different steps of the process of the invention (defined in the table below the figure) (C). The SGC purified pool consisting of SGC fractions F7-F11 is shown in lane 12.

FIG. 12: SEC area (mAU*min; right axis) and TCID.sub.50 results (log TCID50/mL; left axis) of attenuated .DELTA.5nsP3 ChikV production harvests before and after PS treatment. The grey portions of the bars indicate large losses in SEC area following PS treatment, but no corresponding change in the total number of infectious particles (indicated by black portions of the bars) (A); SEC profile of virus preparation before and after PS addition, showing a complete removal of large size virus aggregates by PS treatment as well as a reduction in host cell proteins (HCP) and LMW impurities (B).

FIG. 13: Electron micrographs of attenuated .DELTA.5nsP3 ChikV harvest before and after PS treatment.

FIG. 14: Preparation of the sucrose gradient.

FIG. 15: Comparison of four different sucrose gradient centrifugation experiments performed to empirically determine the optimal combination of sucrose layers for CHIKV purification. The CHIKV content in the gradient fractions was determined by SEC. The sucrose content in the gradient fractions was determined by refractometry (comparing the value of the refractive index of the sucrose solution to that of sucrose standard curve the concentration of sucrose solution can be determined with good accuracy, this is also referred to as "Brix" scale that is calibrated to give the percentage (w/w) of sucrose dissolved in water, i.e. ".degree. Bx"). Protamine sulphate (PS) was determined by SEC. PS is separated within the sucrose gradient alongside host cell derived residual contaminants and was therefore used to assess the quality of CHIKV separation from residual contaminants in the tested gradients. A: CHIKV load material containing 10% sucrose was loaded on top of one 50% (w/w) sucrose layer. Determination of sucrose content in the fractions showed the formation of a linear gradient. SEC showed concentration of CHIKV within a sucrose concentration range from 40-30% (w/w) sucrose. PS SEC showed insufficient separation of PS from CHIKV. B: CHIKV load material containing 10% sucrose was loaded on top of a two layer system consisting of a 50% (w/w) sucrose bottom layer and a second 35% (w/w) sucrose layer. Determination of sucrose content in the fractions showed the formation of a linear gradient. SEC showed concentration of CHIKV within a sucrose concentration range from 40-30% (w/w) sucrose. PS SEC showed acceptable separation of PS from CHIKV, however a slight overlap is still present. C: CHIKV load material containing 10% sucrose was loaded on top of a two layer system consisting of a 50% (w/w) sucrose bottom layer and a second 25% (w/w) sucrose layer. Determination of sucrose content in the fractions showed the formation of a linear gradient. SEC showed concentration of CHIKV within a sucrose concentration range from 40-30% (w/w) sucrose. PS SEC showed a good separation of PS from CHIKV. D: CHIKV load material containing 10% sucrose was loaded on top of a three layer system consisting of a 50% (w/w) sucrose bottom layer as well as a 35% and a 15% (w/w) sucrose layer. Determination of sucrose content in the fractions showed the formation of a linear gradient and SEC showed concentration of CHIKV within a sucrose concentration range from 40-30% (w/w) sucrose. PS SEC showed a very good separation of PS and residual contaminants from CHIKV. Of the four tested sucrose layer systems the combination of 3 layers (shown in FIG. 16D) showed the best separation of the virus particles from residual contaminants and was therefore used for further DSP development.

FIG. 16: Relative amounts of attenuated .DELTA.5nsP3 ChikV particles and other components by SEC-HPLC analysis at the different steps of the process of the invention including, from top to bottom: crude harvest (a); 10.times. concentrated harvest; diafiltrated concentrated harvest; PS treated material; CC700-treated material and SGC purified pool.

FIG. 17: An exemplary downstream virus purification process from the crude harvest to formulation of the drug substance (vaccine), a preferred embodiment of the process of the invention (A). A flow-chart of an exemplary virus inactivation process is shown in (B).

FIG. 18: PS treatment results in selective removal of Zika virus aggregates and Vero HCP and LMW impurities (SEC-HPLC of 30.times. concentrated Zika Virus harvest day 5).

FIG. 19: SEC-HPLC of individual 30.times. concentrated Zika harvest prior PS treatment at different time points.

FIG. 20: SEC-HPLC of individual 30.times. concentrated Zika harvest post PS treatment at different time points.

FIG. 21: Representative SDS-PAGE from the sucrose gradient harvest of a Zika purification is shown.

FIG. 22: Correlation between JEV Antigen content in NIV analysed by ELISA and SEC-HPLC (Dionex Ultimate 3000, Superose 6 column).

FIG. 23: Comparison of JEV and ZikaV harvest schedules/yields.

FIG. 24: SEC-HPLC elution profile of ZikaV NIV. Data were processed on Dionex Ultimate 3000/Superose 6 Increase column. Both panels are from the same chromatogram. The upper graph is the complete elution profile; the lower graph is an enlargement of the ZIKAV elution peak.

FIG. 25: SEC-MALLS analysis of inactivated ZikaV.

FIG. 26: Cumulative particle size distribution of Zika NIV.

FIG. 27: Graphical representation of the neutralization of the Zika virus H/PF/2013 with pooled mouse sera. The number of plaques without serum was set to 100%. The EC50 was calculated with the 3-parameter method.

FIG. 28: Graphical representation of the neutralization of the Zika virus MR766 with pooled mouse sera. The number of plaques without serum was set to 100%. The EC50 was calculated with the 3-parameter method.

FIG. 29: Change in SEC profile of Yellow fever virus peak after PS addition according to the invention showing a complete removal of large size aggregates and LMW impurities.

DETAILED DESCRIPTION OF THE INVENTION

Disclosed herein are processes for the purification of infectious virus particles, i.e., mature, functional virus particles, e.g. flavivirus particles (Yellow Fever, Zika Virus, Japanese Encephalitis virus, Dengue virus) and/or alphavirus particles (Chikungunya virus). The processes disclosed are characterized by the removal of undesired by-products of virus production on host cells, such as non-infectious virus particles and aggregated and immature virus by-products. The processes provided herein allow the production of highly-purified virus preparations comprising mostly infectious virus particles. During the course of the invention, it was observed that protamine sulphate (PS), added to remove contaminating DNA during virus purification, resulted not only in removal of contaminating DNA, but also in the loss of a high percentage of total virus particles present in the preparation. Surprisingly, however, quantification of total infectious virus particles by TCID50 before and after PS treatment revealed that the absolute number of infectious virus particles did not change following this loss of total virus particles. This observation clearly shows that treatment with PS can facilitate selective removal of non-infectious, aggregated and immature viral by-products, leaving behind the infectious Chikungunya virus particles or other infectious virus particles. Because by-products produced during virus growth on host cells may have different (and undesirable) immunological properties or other unwanted side-effects or safety issues, a simple and robust way to remove these by-products is of high importance for the quality and safety of the final product.

Protamines are small arginine-rich nuclear proteins, present in high amounts in the sperm of fish, which have an important role in DNA packaging during spermatogenesis. Protamine sulfate (or "protamine" or "PS") can form a stable ion pair with heparin and is thus commonly used during certain surgeries when the anti-coagulation effect of heparin is no longer needed. In large doses, protamine sulfate administered alone can also have a weak anticoagulant effect ("Protamine sulfate". Wikipedia: The Free Encyclopedia. Wikimedia Foundation, Inc. 30 Sep. 2015 Web. 26 Nov. 2015<https://en.wikipedia.org/wiki/Protamine_sulfate>). Protamine Sulphate is additionally routinely used in biotechnology applications such as DNA precipitation (e.g., removal of host cell DNA from cell culture processes), purification of DNA binding proteins and retroviral-mediated gene transfer.

Protamine is obtained from salmon sperm or produced recombinantly and is used as a sulphate salt. The four major peptides, which constitute almost the entire nitrogen-containing material in salmon protamine, have been fully characterized and found to be polypeptides of 30-32 amino acids in length, of which 21-22 residues are arginine. The average molecular mass is in the range of 4250 Da for the following sequence: PRRRRSSSRP VRRRRRPRVS RRRRRRGGRR RR (SEQ ID NO: 1). Herein, protamine is also referred to as protamine salt, or preferably protamine sulphate.

The present invention relates to the use of protamine sulphate (PS) in a process of purification of a live virus, wherein the protamine sulphate facilitates the removal of impurities from a crude virus harvest, including non-infectious virus particles and aggregates. As seen in FIG. 8 using flaviviruses as an example, virus production in the host cell can result in the release of virus products which are not mature, and non-infectious particles, which can also be considered impurities according to the present invention. As such, the present invention also relates to the enrichment of infectious virus particles from a crude harvest containing a mixture of virus particles and other viral products in various stages of maturation.

The use of protamine sulphate can follow crude cell lysis or any further step after cell lysis (e.g. including after a pre-purification with filtration, chromatography etc) wherein the virus particles are further enriched or concentrated and/or other impurities are removed and/or buffer components are exchanged. The further steps may comprise filtration or concentration of the crude cell lysate.

The protamine sulphate may comprise the sequence PRRRRSSSRP VRRRRRPRVS RRRRRRGGRR RR (SEQ ID NO: 1) or a variant thereof wherein the amino acid sequence comprises from 28-35 amino acids, preferably 29-34, more preferably 30-33 amino acids, most preferably 31 or 32 amino acids. The protamine sulphate preferably comprises at least 19 arginine residues, more preferably at least 20 arginine residues, more preferably at least 21 arginine residues, even more preferably at least 22 residues, most preferably 20 or 21 arginine residues. Further, other protamine sulphate-like compounds or variants thereof may be used. Therefore, the use of the term "protamine salt" herein shall serve to encompass natural variations on SEQ ID NO: 1, preferably, but not limited to, the protamine sulphate forms.

The process according to the current invention may also comprise the use of a sucrose gradient, preferably an optimized sucrose gradient. The sucrose gradient is preferably optimized for the removal of protamine sulfate, also for the removal of immature viral particles or other viral particles which are non-infectious or host cell proteins or nucleic acids (DNA, RNA, mRNA, etc) or other host cell debris. In the current invention the optimized sucrose gradient comprises at least two, at least three, at least four layers of sucrose solutions with different densities. In one embodiment, the virus preparation to be purified is provided in a sucrose solution which has a density of about 8%, about 9%, about 10%, about 11%, about 12% sucrose (w/w), preferably about 10%. In one embodiment, one sucrose solution in the gradient has a density of about 45%, about 46%, about 47%, about 48%, about 49%, about 50%, about 51%, about 52%, about 53%, about 54%, about 55% sucrose (w/w), preferably about 50%. In one embodiment, one sucrose solution in the gradient has a density of about 30%, about 31%, about 32%, about 33%, about 34%, about 35%, about 36%, about 37%, about 38%, about 39%, about 40% sucrose (w/w), preferably about 35%. In one embodiment, one sucrose solution in the gradient has a density of about 10%, about 11%, about 12%, about 13%, about 14%, about 15%, about 16%, about 17%, about 18%, about 19%, about 20% sucrose (w/w), preferably about 15% sucrose. In a preferred embodiment, the sucrose gradient comprises three layers of sucrose solutions of about 50%, about 35% and about 15% (w/w) sucrose and the virus composition to be purified is contained in about 10% (w/w) sucrose. Because the invention provided for means to not only test for host cell DNA but also immature viral particles, the skilled person in the art is able to more precisely optimize the sucrose gradient for most efficient purification and include additional tools such as PRNT assay to monitor purification success.

The process comprising the use of protamine sulphate of the invention can be applied to purification of any virus for use in pharmaceutical compositions, for example, for a pharmaceutical composition such as a vaccine where it is important that the virus is in its infectious form. The virus to be purified may be any live virus, any live attenuated virus or any live chimeric virus, preferably a live wild type virus such as a Zika virus of the Asian lineage. In one embodiment, the virus particle is also be later inactivated. In a preferred embodiment, the virus is inactivated with formaldehyde.

In a preferred embodiment, the produced Zika virus is derived from the Asian lineage (which includes the strains found in South America and all strains derived from any Asian lineage). In some other embodiments, the produced Zika virus is a Zika virus as described in the Sequence section of this application (SEQ ID NO: 2 to 69 or 78).

In another preferred embodiment, the live attenuated Chikungunya virus is the protective ChikV-ICRES1-.DELTA.5nsP3 described by Hallengard et al. (Novel Attenuated Chikungunya Vaccine Candidates Elicit Protective Immunity in C57BL/6 mice (2014) J. Virology, 88(5):2858-2866). Briefly, the ChikV genome carries a positive-sense single-stranded RNA genome of 11 Kb containing two open reading frames encoding nonstructural proteins (nsP1 to nsP4) and structural proteins (C, E3, E2, 6K, and E1), respectively (see FIG. 9, top construct). The attenuated virus .DELTA.5nsP3, based on the La Reunion ChikV strain LR2006-OPY1, was obtained by the substitution of amino acid residues 1656 to 1717 of the P1234 polyprotein with a small linker (aa sequence AYRAAAG) in the hypervariable region of the nsP3 protein (see FIG. 9, bottom construct). The .DELTA.5nsP3 ChikV mutant was shown to be infectious, highly immunogenic and protective against challenge with wild type ChikV (Hallengard, et al., supra and Hallengard, et al., Prime-Boost Immunization Strategies against Chikungunya Virus (2014) J. Virology, 88(22):13333-13343). In one embodiment, the live attenuated Chikungunya virus may be a variant of the ChikV-ICRES1-.DELTA.5nsP3 attenuated mutant virus.

A preferred embodiment of the process of the current invention is shown in FIG. 10 (Chikungunya virus) and FIG. 17A (Zika virus).

TABLE-US-00001 TABLE 1 Overview of process buffers and stock solutions Final conductivity Buffer Composition Final pH [mS/cm] A 0.5M NaOH n.a. B 0.1M NaOH n.a. C 25 mM Tris, 150 mM NaCl 7.4 .+-. 0.2 16.5 D 1M Tris 7.4 .+-. 0.2 n.a. E 4.5M NaCl n.a. n.a. F 1M NaCl n.a. n.a. G 1% SDS n.a. n.a. H 50% (w/w) Sucrose in 25 mM 7.4 .+-. 0.2 n.a. Tris, 150 mM NaCl I 35% (w/w) Sucrose in 25 mM 7.4 .+-. 0.2 n.a. Tris, 150 mM NaCl J 15% (w/w) Sucrose in 25 mM 7.4 .+-. 0.2 n.a. Tris, 150 mM NaCl K 10.times. PBS 7.4 .+-. 0.2 n.a. L 50 mg/mL Protamine sulphate 7.4 .+-. 0.2 n.a. M Drug substance formulation 7.4 .+-. 0.2 1.3 buffer (10 mM Tris(hydroxymethyl)- aminomethan, 5% Sucrose, 1% (10 mg/mL) rHSA)

TABLE-US-00002 TABLE 2 Abbreviations Degrees Brix = sugar content .degree.Bx (w/w) of an aqueous solution BSA Bovine serum albumin CC700 Capto .TM. Core 700 ChikV Chikungunya virus CPE Cytopathic effect EtOH Ethanol EU Endotoxin units DS Drug Substance DP Drug Product DSP Downstream Process HCP Host cell protein hcDNA Host cell DNA hpi Hours post infection HPLC High Performance Liquid Chromatography ID Inner diameter JEV Japanese Encephalitis virus LAL Limulus amebocyte lysate LDS buffer Lithium dodecyl sulfate sample loading buffer LOD Limit of detection LOQ Limit of quantitation MALLS Multiangle light scattering mAU Milli absorbance units MS Mass spectroscopy NIV Neutralized inactivated virus PBS Phosphate buffered saline PD Process development PFU Plaque forming units p.i. Post-infection PS Protamine sulphate or protamine sulfate rcf Relative centrifugal force rHSA Recombinant human serum albumin Rms radius Root mean square radius rMSB Research master seed bank RSD Relative standard deviation SEC Size exclusion chromatography SGC Sucrose gradient centrifugation SGP Sucrose gradient purified SDS Sodium dodecyl sulphate TBS Tris buffered saline TFF Tangential flow filtration TCID50 Tissue culture infectious dose 50% UF/DF Ultrafiltration/diafiltration WFI Water for injection ZikaV Zika virus

Brix:

Degrees Brix (.degree. Bx) is the sugar content of an aqueous solution. One degree Brix is 1 gram of sucrose in 100 grams of solution and represents the strength of the solution as percentage by mass. .degree. Bx corresponds to the sucrose content in % (w/w), eg. 45.degree. Bx equals 45% (w/w) sucrose.

TABLE-US-00003 TABLE A Primers for Zika virus sequencing: lower case letters indicate bases not included in ZIKA but containing restriction sites for later cloning when needed (therefore, two Tms provided). Tm Primer Primer sequence (5'-3') (gene- Tm (entire Amplicon Pair Oligoname restriction sites (lower case) specific) primer) size [bp] 1 9320_Zika_PF_1F SEQ ID NO: 80 69.9 74.6 707 ttaggatccGTTGTTGATCTGTGTGAAT 9321_Zika_PF_1R SEQ ID NO: 81 69.3 75.6 taactcgagCGTACACAACCCAAGTT 2 9322_Zika_PF_2F SEQ ID NO: 82 70 73.9 704 ttaggatccTCACTAGACGTGGGAGTG 9323_Zika_PF_2R SEQ ID NO: 83 69.8 73.7 taactcgagAAGCCATGTCYGATATTGAT 3 9324_Zika_PF_3F SEQ ID NO: 84 72.3 74.5 712 ttaggatccGCATACAGCATCAGGTG 9325_Zika_PF_3R SEQ ID NO: 85 72 76.4 taactcgagTGTGGAGTTCCGGTGTCT 4 9326_Zika_PF_4F SEQ ID NO: 86 70.9 74 712 ttaggatccGAATAGAGCGAARGTTGAGATA 9327_Zika_PF_4R SEQ ID NO: 87 70.5 73.7 taactcgAGTGGTGGGTGATCTTCTTCT 5 9328_Zika_PF_5F SEQ ID NO: 88 70.3 75 704 ttaggatcCAGTCACAGTGGAGGTACAGTAC 9329_Zika_PF_5R SEQ ID NO: 89 71.5 77.3 taactcgagCRCAGATACCATCTTCCC 6 9330_Zika_PF_6F SEQ ID NO: 90 70.7 72.7 698 ttaggatCCCTTATGTGCTTGGCCTTAG 9331_Zika_PF_6R SEQ ID NO: 91 70.4 76.9 taactcgagTCTTCAGCCTCCATGTG 7 9332_Zika_PF_7F SEQ ID NO: 92 71.9 75 716 ttaggatccAATGCCCACTCAAACATAGA 9333_Zika_PF_7R SEQ ID NO: 93 71 74 taactcgagTCATTCTCTTCTTCAGCCCTT 8 9334_Zika_PF_8F SEQ ID NO: 94 70.9 75.2 703 ttaggatccAAGGGTGATCGAGGAAT 9335_Zika_PF_8R SEQ ID NO: 95 71.9 73.4 taactcgagTTCCCTTCAGAGAGAGGAGC 9 9336_Zika_PF_9F SEQ ID NO: 96 71.9 75 699 ttaggatccTCTTTTGCAAACTGCGATC 9337_Zika_PF_9R SEQ ID NO: 97 71 74.9 taactcgagTCCAGCTGCAAAGGGTAT 10 9338_Zika_PF_10F SEQ ID NO: 98 71.4 75.8 706 ttaggatccGTGTGGACATGTACATTGA 9339_Zika_PF_10R SEQ ID NO: 99 70.4 75.8 taactcgagCCCATTGCCATAAAGTC 11 9340_Zika_PF_11F SEQ ID NO: 100 71.6 78.1 692 ttaggatccTCATACTGTGGTCCATGGA 9341_Zika_PF_11R SEQ ID NO: 101 74 78 taactcgagGCCCATCTCAACCCTTG 12 9342_Zika_PF_12F SEQ ID NO: 102 70.9 74 707 ttaggatccTAGAGGGCTTCCAGTGC 9343_Zika_PF_12R SEQ ID NO: 103 70.2 72.2 taactcgAGATACTCATCTCCAGGTTTGTTG 13 9344_Zika_PF_13F SEQ ID NO: 104 70.6 75.4 726 ttaggatccGAAAACAAAACATCAAGAGTG 9345_Zika_PF_13R SEQ ID NO: 105 71.9 75.6 taactcgagGAATCTCTCTGTCATGTGTCCT 14 9346_Zika_PF_14F SEQ ID NO: 106 73.1 75.6 715 ttaggatccTTGATGGCACGACCAAC 9347_Zika_PF_14R SEQ ID NO: 107 70.8 77.9 ttaggatccGTTGTTGATCTGTGTGAAT 15 9348_Zika_PF_15F SEQ ID NO: 108 71.9 75.4 719 taactcgagCAGGTCAATGTCCATTG 9349_Zika_PF_15R SEQ ID NO: 109 73.9 77.2 ttaggatccTGTTGTGTTCCTATTGCTGGT 16 9350_Zika_PF_16F SEQ ID NO: 110 72.3 75.4 703 taactcgaGTGATCAGRGCCCCAGC 9351_Zika_PF_16R SEQ ID NO: 111 72 76.3 ttaggatccTGCTGCCCAGAAGAGAA 17 9352_Zika_PF_17F SEQ ID NO: 112 73.6 76 705 taactcgaGCACCAACAYGGGTTCTT 9353_Zika_PF_17R SEQ ID NO: 113 72 75.5 ttaggatcCTCAAGGACGGTGTGGC 18 9354_Zika_PF_18F SEQ ID NO: 114 71.7 75.8 699 taactcgagCAATGATCTTCATGTTGGG 9355_Zika_PF_18R SEQ ID NO: 115 71 74.1 ttaggatccTATGGGGGAGGACTGGT 19 9356_Zika_PF_19F SEQ ID NO: 116 73.3 75.5 711 taactcGAGCCCAGAACCTTGGATC 9357_Zika_PF_19R SEQ ID NO: 117 71.3 76.9 ttaggatcCAGACCCCCAAGAAGGC 20 9358_Zika_PF_20F SEQ ID NO: 118 71.7 75 706 taactcgagCCCCTTTGGTCTTGTCT 9359_Zika_PF_20R SEQ ID NO: 119 71.9 73.9 ttaggatccAGGAAGGATGTATGCAGATG 21 9360_Zika_PF_21F SEQ ID NO: 120 70.4 75.7 709 taactcgagACATTTGCGCATATGATTTTG 9361_Zika_PF_21R SEQ ID NO: 121 71.8 75 ttaggatccAGGAAGGACACACAAGAGT 22 9362_Zika_PF_22F SEQ ID NO: 122 70 79.1 581 taactcgagACAGGCTGCACAGCTTT 9363_Zika_PF_22R SEQ ID NO: 123 74.8 81.1 ttaggatccTCTCTCATAGGGCACAGAC

Sequences

TABLE-US-00004 A typical form of protamine SEQ ID NO: 1 PRRRRSSSRP VRRRRRPRVS RRRRRRGGRR RR

Provided below are examples of nucleic acid sequences of the genomes of Zika viruses that may be used in the methods, compositions, and/or vaccines described herein.

TABLE-US-00005 KU321639.1 Zika virus strain ZikaSPH2015, Brazil, complete genome (SEQ ID NO: 2) SEQ ID NO: 2 GTTGTTACTGTTGCTGACTCAGACTGCGACAGTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTT- TATTTGGATT TGGAAACGAGAGTTTCTGGTCATGAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATATGCTAAA- ACGCGGAG TAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGTCATGGGCCCATCAGG- ATGGTCTTG GCAATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAGATGGGGTTCAGTGGG- GAAAAAAGA GGCTATGGAAATAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGCTGAGAATAATCAATGCTAGGAAGGAGA- AGAAGAG ACGGGGCGCAGATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGCGGAGGTCACTAGAC- GTGGGAGT GCATACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAA- GTGTTATAT ACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGGTGG- AACCAGAT GACGTCGATTGTTGGTGCAACACGACGTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAAAAGGTGAAGC- ACGGAGAT CTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACTAGGAAGCTGCAAACGCGGTCGCAAACCTGGTTGGAATCA- AGAGAATAC ACAAAGCACTTGATTAGAGTCGAAAATTGGATATTCAGGAACCCTGGCTTCGCGTTAGCAGCAGCTGCCATCGC- TTGGCTTTTG GGAAGCTCAACGAGCCAAAAAGTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTG- CATAGGAGT CAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTGATATTGTCTTGGAACATGGAGGTTGTG- TCACCGTA ATGGCACAGGACAAACCGACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGTAAGATC- CTACTGCT ATGAGGCATCAATATCAGACATGGCTTCGGACAGCCGCTGCCCAACACAAGGTGAAGCCTACCTTGACAAGCAA- TCAGACACT CAATATGTCTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGTCT- GGTGACAT GCGCTAAGTTTGCATGCTCCAAGAAAATGACCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAATG- CTGTCAGT TCATGGCTCCCAGCACAGTGGGATGATCGTTAATGACACAGGACATGAAACTGATGAGAATAGAGCGAAGGTTG- AGATAACG CCCAATTCACCAAGAGCCGAAGCCACCCTGGGGGGTTTTGGAAGCCTAGGACTTGATTGTGAACCGAGGACAGG- CCTTGACTT TTCAGATTTGTATTACTTGACTATGAATAACAAGCACTGGTTGGTTCACAAGGAGTGGTTCCACGACATTCCAT- TACCTTGGCAC GCTGGGGCAGACACCGGAACTCCACACTGGAACAACAAAGAAGCACTGGTAGAGTTCAAGGACGCACATGCCAA- AAGGCAA ACTGTCGTGGTTCTAGGGAGTCAAGAAGGAGCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGGA- TGGTGCAA AGGGAAGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATAAACTTAGATTGAAGGGCGTGTCATAC- TCCTTGTGT ACCGCAGCGTTCACATTCACCAAGATCCCGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGTACAGTACGC- AGGGACAG ATGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGACATGCAAACTCTGACCCCAGTTGGGAGGTTGATAACC- GCTAACCCC GTAATCACTGAAAGCACTGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACATTGT- CATAGGAGTC GGGGAGAAGAAGATCACCCACCACTGGCACAGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGAG- AGGTGCC AAGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGTTGGAGGCGCTCTCAACTCATTGGGCAA- GGGCATCC ATCAAATTTTTGGAGCAGCTTTCAAATCATTGTTTGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGAACG- TTGCTGATGTG GTTGGGTCTGAACACAAAGAATGGATCTATTTCCCTTATGTGCTTGGCCTTAGGGGGAGTGTTGATCTTCTTAT- CCACAGCCGT CTCTGCTGATGTGGGGTGCTCGGTGGACTTCTCAAAGAAGGAGACGAGATGCGGTACAGGGGTGTTCGTCTATA- ACGACGTT GAAGCCTGGAGGGACAGGTACAAGTACCATCCTGACTCCCCCCGTAGATTGGCAGCAGCAGTCAAGCAAGCCTG- GGAAGATG GTATCTGCGGGATCTCCTCTGTTTCAAGAATGGAAAACATCATGTGGAGATCAGTAGAAGGGGAGCTCAACGCA- ATCCTGGAA GAGAATGGAGTTCAACTGACGGTCGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATTGCC- CGTGCCTG TGAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCGCACTTCGTCAGAGCAGCAAAGACAAATAACAGC- TTTGTCGT GGATGGTGACACACTGAAGGAATGCCCACTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAGGATCATGGGT- TCGGGGTA TTTCACACTAGTGTCTGGCTCAAGGTTAGAGAAGATTATTCATTAGAGTGTGATCCAGCCGTTATTGGAACAGC- TGTTAAGGGA AAGGAGGCTGTACACAGTGATCTAGGCTACTGGATTGAGAGTGAGAAGAATGACACATGGAGGCTGAAGAGGGC- CCATCTG ATCGAGATGAAAACATGTGAATGGCCAAAGTCCCACACATTGTGGACAGATGGAATAGAAGAGAGTGATCTGAT- CATACCCA AGTCTTTAGCTGGGCCACTCAGCCATCACAATACCAGAGAGGGCTACAGGACCCAAATGAAAGGGCCATGGCAC- AGTGAAGA GCTTGAAATTCGGTTTGAGGAATGCCCAGGCACTAAGGTCCACGTGGAGGAAACATGTGGAACAAGAGGACCAT- CTCTGAGA TCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGTGCTGCAGGGAGTGCACAATGCCCCCACTGTCGTTCCG- GGCTAAAG ATGGCTGTTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAAGCAACTTAGTAAGGTCAATGGTGACT- GCAGGATC AACTGATCACATGGATCACTTCTCCCTTGGAGTGCTTGTGATTCTGCTCATGGTGCAGGAAGGGCTGAAGAAGA- GAATGACCA CAAAGATCATCATAAGCACATCAATGGCAGTGCTGGTAGCTATGATCCTGGGAGGATTTTCAATGAGTGACCTG- GCTAAGCTT GCAATTTTGATGGGTGCCACCTTCGCGGAAATGAACACTGGAGGAGATGTAGCTCATCTGGCGCTGATAGCGGC- ATTCAAAGT CAGACCAGCGTTGCTGGTATCTTTCATCTTCAGAGCTAATTGGACACCCCGTGAAAGCATGCTGCTGGCCTTGG- CCTCGTGTCT TTTGCAAACTGCGATCTCCGCCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGGTTGG- CAATACGAGC GATGGTTGTTCCACGCACTGATAACATCACCTTGGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCACAC- TGCTTGTGGC GTGGAGAGCAGGCCTTGCTACTTGCGGGGGGTTTATGCTCCTCTCTCTGAAGGGAAAAGGCAGTGTGAAGAAGA- ACTTACCA TTTGTCATGGCCCTGGGACTAACCGCTGTGAGGCTGGTCGACCCCATCAACGTGGTGGGGCTGCTGTTGCTCAC- AAGGAGTGG GAAGCGGAGCTGGCCCCCTAGCGAAGTACTCACAGCTGTTGGCCTGATATGCGCATTGGCTGGAGGGTTCGCCA- AGGCAGAT ATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAGGAAAGAGTGTGGA- CATGTACA TTGAAAGAGCAGGTGACATCACATGGGAAAAAGATGCGGAAGTCACTGGAAACAGTCCCCGGCTCGATGTGGCG- CTAGATGA GAGTGGTGATTTCTCCCTGGTGGAGGATGACGGTCCCCCCATGAGAGAGATCATACTCAAGGTGGTCCTGATGA- CCATCTGTG GCATGAACCCAATAGCCATACCCTTTGCAGCTGGAGCGTGGTACGTATACGTGAAGACTGGAAAAAGGAGTGGT- GCTCTATG GGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCACAGATGGAGTGTACAGAGTAATGACTCGTAGAC- TGCTAGG TTCAACACAAGTTGGAGTGGGAGTTATGCAAGAGGGGGTCTTTCACACTATGTGGCACGTCACAAAAGGATCCG- CGCTGAGA AGCGGTGAAGGGAGACTTGATCCATACTGGGGAGATGTCAAGCAGGATCTGGTGTCATACTGTGGTCCATGGAA- GCTAGATG CCGCCTGGGACGGGCACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGAGAGCGAGGAACATCCAGACT- CTGCCCG GAATATTTAAGACAAAGGATGGGGACATTGGAGCGGTTGCGCTGGATTACCCAGCAGGAACTTCAGGATCTCCA- ATCCTAGA CAAGTGTGGGAGAGTGATAGGACTTTATGGCAATGGGGTCGTGATCAAAAATGGGAGTTATGTTAGTGCCATCA- CCCAAGGG AGGAGGGAGGAAGAGACTCCTGTTGAGTGCTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTAACTGTCTTAGA- CTTGCATC CTGGAGCTGGGAAAACCAGGAGAGTTCTTCCTGAAATAGTCCGTGAAGCCATAAAAACAAGACTCCGTACTGTG- ATCTTAGCT CCAACCAGGGTTGTCGCTGCTGAAATGGAGGAAGCCCTTAGAGGGCTTCCAGTGCGTTATATGACAACAGCAGT- CAATGTCAC CCACTCTGGAACAGAAATCGTCGACTTAATGTGCCATGCCACCTTCACTTCACGTCTACTACAGCCAATCAGAG- TCCCCAACTAT AATCTGTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAAGAGGATACATTTCAACAAG- GGTTGAGAT GGGCGAGGCGGCTGCCATCTTCATGACCGCCACGCCACCAGGAACCCGTGACGCATTTCCGGACTCCAACTCAC- CAATTATGG ACACCGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTCAGGCTTTGATTGGGTGACGGATTATTCTGGAAAAACA- GTTTGGTT TGTTCCAAGCGTGAGGAACGGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAACGGGTCATACAGCTCA- GCAGAAAG ACTTTTGAGACAGAGTTCCAGAAAACAAAACATCAAGAGTGGGACTTTGTCGTGACAACTGACATTTCAGAGAT- GGGCGCCAA CTTTAAAGCTGACCGTGTCATAGATTCCAGGAGATGCCTAAAGCCGGTCATACTTGATGGCGAGAGAGTCATTC- TGGCTGGAC CCATGCCTGTCACACATGCCAGCGCTGCCCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGGAGAT- GAGTATCT GTATGGAGGTGGGTGCGCAGAGACTGACGAAGACCATGCACACTGGCTTGAAGCAAGAATGCTCCTTGACAATA- TTTACCTCC AAGATGGCCTCATAGCCTCGCTCTATCGACCTGAGGCCGACAAAGTAGCAGCCATTGAGGGAGAGTTCAAGCTT- AGGACGGA GCAAAGGAAGACCTTTGTGGAACTCATGAAAAGAGGAGATCTTCCTGTTTGGCTGGCCTATCAGGTTGCATCTG- CCGGAATAA CCTACACAGATAGAAGATGGTGCTTTGATGGCACGACCAACAACACCATAATGGAAGACAGTGTGCCGGCAGAG- GTGTGGAC CAGACACGGAGAGAAAAGAGTGCTCAAACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCCTGA- AGTCATTC AAGGAGTTTGCCGCTGGGAAAAGAGGAGCGGCTTTTGGAGTGATGGAAGCCCTGGGAACACTGCCAGGACACAT- GACAGAG AGATTCCAGGAAGCCATTGACAACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGCCTTACAAAGCCGC- GGCGGCCC AATTGCCGGAGACCCTAGAGACCATTATGCTTTTGGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTTTTCGTC- TTGATGAGG AACAAGGGCATAGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGCGCATGGCTCATGTGGCTCTCGGA- AATTGAGC CAGCCAGAATTGCATGTGTCCTCATTGTTGTGTTCCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAA-

AGATCTCCCC AGGACAACCAAATGGCAATCATCATCATGGTAGCAGTAGGTCTTCTGGGCTTGATTACCGCCAATGAACTCGGA- TGGTTGGAG AGAACAAAGAGTGACCTAAGCCATCTAATGGGAAGGAGAGAGGAGGGGGCAACCATGGGATTCTCAATGGACAT- TGACCTG CGGCCAGCCTCAGCTTGGGCCATCTATGCTGCCTTGACAACTTTCATTACCCCAGCCGTCCAACATGCAGTGAC- CACTTCATACA ACAACTACTCCTTAATGGCGATGGCCACGCAAGCTGGAGTGTTGTTTGGTATGGGCAAAGGGATGCCATTCTAC- GCATGGGAC TTTGGAGTCCCGCTGCTAATGATAGGTTGCTACTCACAATTAACGCCCCTGACCCTAATAGTGGCCATCATTTT- GCTCGTGGCG CACTACATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCGTGCTGCCCAGAAGAGAACGGCAGCTGGCAT- CATGAAGA ACCCTGTTGTGGATGGAATAGTGGTGACTGACATTGACACAATGACAATTGACCCCCAAGTGGAGAAAAAGATG- GGACAGGT GCTACTCATGGCAGTAGCCGTCTCCAGCGCCATACTGTCGCGGACCGCCTGGGGGTGGGGGGAGGCTGGGGCCC- TGATCACA GCCGCAACTTCCACTTTGTGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCTACAGCCACTTCACTGTGTAA- CATTTTTAGG GGAAGTTACTTGGCTGGAGCTTCTCTAATCTACACAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGG- TGGAACAG GAGAGACCCTGGGAGAGAAATGGAAGGCCCGCTTGAACCAGATGTCGGCCCTGGAGTTCTACTCCTACAAAAAG- TCAGGCAT CACCGAGGTGTGCAGAGAAGAGGCCCGCCGCGCCCTCAAGGACGGTGTGGCAACGGGAGGCCATGCTGTGTCCC- GAGGAAG TGCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCTGCAGCCCTATGGAAAGGTCATTGATCTTGGATGTGGCA- GAGGGGG CTGGAGTTACTACGCCGCCACCATCCGCAAAGTTCAAGAAGTGAAAGGATACACAAAAGGAGGCCCTGGTCATG- AAGAACCC GTGTTGGTGCAAAGCTATGGGTGGAACATAGTCCGTCTTAAGAGTGGGGTGGACGTCTTTCATATGGCGGCTGA- GCCGTGTG ACACGTTGCTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAAGCACGGACGCTCAGAGTCCTC- TCCATGGTG GGGGATTGGCTTGAAAAAAGACCAGGAGCCTTTTGTATAAAAGTGTTGTGCCCATACACCAGCACTATGATGGA- AACCCTGGA GCGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGAGTGCCACTCTCCCGCAACTCTACACATGAGATGTACT- GGGTCTCT GGAGCGAAAAGCAACACCATAAAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCGCATGGACGGGCCTAG- GAGGCCA GTGAAATATGAGGAGGATGTGAATCTCGGCTCTGGCACGCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACAT- GAAGATCA TTGGTAACCGCATTGAAAGGATCCGCAGTGAGCACGCGGAAACGTGGTTCTTTGACGAGAACCACCCATATAGG- ACATGGGC TTACCATGGAAGCTATGAGGCCCCCACACAAGGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGT- CAAAACCCT GGGATGTGGTGACTGGAGTCACAGGAATAGCCATGACCGACACCACACCGTATGGTCAGCAAAGAGTTTTCAAG- GAAAAAGT GGACACTAGGGTGCCAGACCCCCAAGAAGGTACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTTGTGGAAAG- AGCTAGGC AAACACAAACGGCCACGAGTCTGTACCAAAGAAGAGTTCATCAACAAGGTTCGTAGCAATGCAGCATTAGGGGC- AATATTTGA AGAGGAAAAAGAGTGGAAGACTGCAGTGGAAGCTGTGAACGATCCAAGGTTCTGGGCTCTAGTGGACAAGGAAA- GAGAGCA CCACCTGAGAGGAGAGTGCCAGAGTTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAACAAGGGGAATTTG- GAAAGGC CAAGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAGATTTCTAGAGTTCGAAGCCCTTGGATTCTTGA- ACGAGGATC ACTGGATGGGGAGAGAGAACTCAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGATATGTCCTAGAA- GAGATGA GTCGCATACCAGGAGGAAGGATGTATGCAGATGACACTGCTGGCTGGGACACCCGCATCAGCAGGTTTGATCTG- GAGAATGA AGCTCTAATCACCAACCAAATGGAGAAAGGGCACAGGGCCTTGGCATTGGCCATAATCAAGTACACATACCAAA- ACAAAGTG GTAAAGGTCCTTAGACCAGCTGAAAAAGGGAAAACAGTTATGGACATTATTTCGAGACAAGACCAAAGGGGGAG- CGGACAA GTTGTCACTTACGCTCTTAACACATTTACCAACCTAGTGGTGCAACTCATTCGGAATATGGAGGCTGAGGAAGT- CCTAGAGATG CAAGACTTGTGGCTGCTGCGGAGGTCAGAGAAAGTGACCAACTGGTTGCAGAGCAACGGATGGGATAGGCTCAA- ACGAATG GCAGTCAGTGGAGATGATTGCGTTGTGAAGCCAATTGATGATAGGTTTGCACATGCCCTCAGGTTCTTGAATGA- TATGGGAAA AGTTAGGAAGGACACACAAGAGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAAGTTCCGTTTTGCTCCC- ACCACTTCA ACAAGCTCCATCTCAAGGACGGGAGGTCCATTGTGGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCC- CGCGTCTCT CCAGGGGCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGCAAATGTGGCAGCTCCTTTA- TTTCCACAG AAGGGACCTCCGACTGATGGCCAATGCCATTTGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGGAGAACTA- CCTGGTCAAT CCATGGAAAGGGAGAATGGATGACCACTGAAGACATGCTTGTGGTGTGGAACAGAGTGTGGATTGAGGAGAACG- ACCACAT GGAAGACAAGACCCCAGTTACGAAATGGACAGACATTCCCTATTTGGGAAAAAGGGAAGACTTGTGGTGTGGAT- CTCTCATA GGGCACAGACCGCGCACCACCTGGGCTGAGAACATTAAAAACACAGTCAACATGGTGCGCAGGATCATAGGTGA- TGAAGAAA AGTACATGGACTACCTATCCACCCAAGTTCGCTACTTGGGTGAAGAAGGGTCTACACCTGGAGTGCTGTAAGCA- CCAATCTTA ATGTTGTCAGGCCTGCTAGTCAGCCACAGCTTGGGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGAAGCTGG- GAAACCAA GCCTATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTGAGCCCCTCAGAGGACACTGAG- TCAAAAAA CCCCACGCGCTTGGAGGCGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAAC- TGGAGATC AGCTGTGGATCTCCAGAAGAGGGACTAGTGGTTAGAGGAGA KU497555.1 Zika virus isolate Brazil-ZKV2015, Brazil, complete genome SEQ ID NO: 3 CCAATCTGTGAATCAGACTGCGACAGTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTTTATTTT- GGATTTGGA AACGAGAGTTTCTGGTCATGAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATATGCTAAAACGC- GGAGTAGC CCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGTCATGGGCCCATCAGGATGG- TCTTGGCG ATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAA- AAAAGAGGC TATGGAAATAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGCTGAGAATAATCAATGCCAGGAAGGAGAAGA- AGAGACG AGGCGCAGATACTAGTGTCGGAATCGTTGGCCTCCTGCTGACCACAGCTATGGCAGCGGAGGTCACTAGACGTG- GGAGTGCA TACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAAGTG- TTATATACA GATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGGTGGAAC- CAGATGAC GTCGATTGTTGGTGCAACACGACGTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAAAAGGTGAAGCACG- GAGATCTA GAAGAGCTGTGACGCTCCCCTCCCATTCCACTAGGAAGCTGCAAACGCGGTCGCAAACCTGGTTGGAATCAAGA- GAATACACA AAGCACTTGATTAGAGTCGAAAATTGGATATTCAGGAACCCTGGCTTCGCGTTAGCAGCAGCTGCCATCGCTTG- GCTTTTGGG AAGCTCAACGAGCCAAAAAGTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCA- TAGGAGTCA GCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTGATGTTGTCTTGGAACATGGGGGTTGTGTC- ACCGTAAT GGCACAGGACAAACCGACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGTAAGATCCT- ACTGCTAT GAGGCATCAATATCAGACATGGCTTCGGACAGCCGCTGCCCAACACAAGGTGAAGCCTACCTTGACAAGCAATC- AGACACTCA ATATGTCTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGCCTGG- TGACATGC GCTAAGTTTGCATGCTCCAAGAAAATGACCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAATGCT- GTCAGTTC ATGGCTCCCAGCACAGTGGGATGATCGTTAATGACACAGGACATGAAACTGATGAGAATAGAGCGAAGGTTGAG- ATAACGCC CAATTCACCAAGAGCCGAAGCCACCCTGGGGGGTTTTGGAAGCTTAGGACTTGATTGTGAACCGAGGACAGGCC- TTGACTTTT CAGATTTGTATTACTTGACTATGAATAACAAGCACTGGTTGGTTCACAAGGAGTGGTTCCACGACATTCCATTA- CCTTGGCACG CTGGGGCAGACACCGGAACTCCACACTGGAACAACAAAGAAGCACTGGTAGAGTTCAAGGACGCACATGCCAAA- AGGCAAA CTGTCGTGGTTCTAGGGACTCAAGAAGGAGCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGGAT- GGTGCAAA GGGAAGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATAAACTTAGATTGAAGGGCGTGTCATACT- CCTTGTGTA CCGCAGCGTTCACATTCACCAAGATCCCGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGTACAGTACGCA- GGGACAGA TGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGACATGCAAACTCTGACCCCAGTTGGGAGGTTGATAACCG- CTAACCCCG TAATCACTGAAAGCACTGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACATTGTC- ATAGGAGTCG GGGAGAAGAAGATCACCCACCACTGGCACAGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGAGA- GGTGCCA AGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGTTGGAGGCGCTCTCAACTCATTGGGCAAG- GGCATCCA TCAAATTTTTGGAGCAGCTTTCAAATCATTGTTTGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGAACGT- TGCTGATGTGG TTGGGTCTGAACACAAAGAATGGATCTATTTCCCTTATGTGCTTGGCCTTAGGGGGAGTGTTGATCTTCTTATC- CACAGCCGTC TCTGCTGATGTGGGGTGCTCGGTGGACTTCTCAAAGAAGGAGACGAGATGTGGTACAGGGGTGTTCGTCTATAA- CGACGTTG AAGCCTGGAGGGACAGGTACAAGTACCATCCTGACTCTCCCCGTAGATTGGCAGCAGCAGTCAAGCAAGCCTGG- GAAGATGG TATCTGCGGGATCTCCTCTGTTTCAAGAATGGAAAACATCATGTGGAGATCAGTAGAAGGGGAGCTTAACGCAA- TCCTGGAAG AGAATGGAGTTCAACTGACGGTCGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATTGCCC- GTGCCTGT GAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCGTACTTCGTCAGAGCAGCAAAGACAAATAACAGCT- TTGTCGTG GATGGTGACACACTGAAGGAATGCCCACTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAGGATCATGGGTT- CGGGGTAT

TTCACACTAGTGTCTGGCTCAAGGTTAGAGAAGATTATTCATTAGAGTGTGATCCAGCCGTTATTGGAACAGCT- GTTAAGGGA AAGGAGGCTGTACACAGTGATCTAGGCTACTGGATTGAGAGTGAGAAGAATGACACATGGAGGCTGAAGAGGGC- CCATCTG ATCGAGATGAAAACATGTGAATGGCCAAAGTCCCACACATTGTGGACAGATGGAATAGAAGAGAGTGATCTGAT- CATACCCA AGTCTTTAGCTGGGCCACTCAGCCATCACAATACCAGAGAGGGCTACAGGACCCAAATGAAAGGGCCATGGCAC- AGTGAAGA GCTTGAAATTCGGTTTGAGGAATGCCCAGGCACTAAGGTCCACGTGGAGGAAACATGTGGAACAAGAGGACCAT- CTCTGAGA TCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGTGCTGCAGGGAGTGCACAATGCCCCCACTGTCGTTCCG- GGCTAAAG ATGGCTGTTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAAGCAACTTAGTAAGGTCAATGGTGACT- GCAGGATC AACTGATCACATGGATCACTTCTCCCTTGGAGTGCTTGTGATTCTGCTCATGGTGCAGGAAGGGCTGAAGAAGA- GAATGACCA CAAAGATCATCATAAGCACATCAATGGCAGTGCTGGTAGCTATGATCCTGGGAGGATTTTCAATGAGTGACCTG- GCTAAGCTT GCAATTTTGATGGGTGCCACCTTCGCGGAAATGAACACTGGAGGAGATGTAGCTCATCTGGCGCTGATAGCGGC- ATTCAAAGT CAGACCAGCGTTGCTGGTATCTTTCATCTTCAGAGCTAATTGGACACCCCGTGAAAGCATGCTGCTGGCCTTGG- CCTCGTGTTT TTTGCAAACTGCGATCTCCGCCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGGTTGG- CAATACGAGC GATGGTTGTTCCACGCACTGACAACATCACCTTGGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCACAC- TGCTTGTGGC GTGGAGAGCAGGCCTTGCTACTTGCGGGGGGTTTATGCTCCTCTCTCTGAAGGGAAAAGGCAGTGTGAAGAAGA- ACTTACCA TTTGTCATGGCCCTGGGACTAACCGCTGTGAGGCTGGTCGACCCCATCAACGTGGTGGGACTGCTGTTGCTCAC- AAGGAGTGG GAAGCGGAGCTGGCCCCCTAGCGAAGTACTCACAGCTGTTGGCCTGATATGCGCATTGGCTGGAGGGTTCGCCA- AGGCAGAT ATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAGGAAAGAGTGTGGA- CATGTACA TTGAAAGAGCAGGTGACATCACATGGGAAAAAGATGCGGAAGTCACTGGAAACAGTCCCCGGCTCGATGTGGCG- CTAGATGA GAGTGGTGACTTCTCCCTGGTGGAGGATGACGGTCCCCCCATGAGAGAGATCATACTCAAGGTGGTCCTGATGA- CCATCTGTG GCATGAACCCAATAGCCATACCCTTTGCAGCTGGAGCGTGGTACGTATACGTGAAGACTGGAAAAAGGAGTGGT- GCTCTATG GGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCACAGATGGAGTGTACAGAGTAATGACTCGTAGAC- TGCTAGG TTCAACACAAGTTGGAGTGGGAGTTATGCAAGAGGGGGTCTTTCACACTATGTGGCACGTCACAAAAGGATCCG- CGCTGAGA AGCGGTGAAGGGAGACTTGATCCATACTGGGGAGATGTCAAGCAGGATCTGGTGTCATACTGTGGTCCATGGAA- GCTAGATG CCGCCTGGGACGGGCACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGAGAGCGAGGAACATCCAGACT- CTGCCCG GAATATTTAAGACAAAGGATGGGGACATTGGAGCGGTTGCGCTGGATTACCCAGCAGGAACTTCAGGATCTCCA- ATCCTAGA CAAGTGTGGGAGAGTGATAGGACTTTATGGCAATGGGGTCGTGATAAAAAATGGGAGTTATGTTAGTGCCATCA- CCCAAGGG AGGAGGGAGGAAGAGACTCCTGTTGAGTGCTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTAACTGTCTTAGA- CTTGCATC CTGGAGCTGGGAAAACCAGGAGAGTTCTTCCTGAAATAGTCCGTGAAGCCATAAAAACAAGACTCCGTACTGTG- ATCTTAGCT CCAACCAGGGTTGTCGCTGCTGAAATGGAGGAAGCCCTTAGAGGGCTTCCAGTGCGTTATATGACAACAGCAGT- CAATGTCAC CCACTCTGGAACAGAAATCGTCGACTTAATGTGCCATGCCACCTTCACTTCACGTCTACTACAGCCAATCAGAG- TCCCCAACTAT AATCTGTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGCATAGCAGCAAGAGGATACATTTCAACAAG- GGTTGAGAT GGGCGAGGCGGCTGCCATCTTCATGACCGCCACGCCACCAGGAACCCGTGACGCATTTCCGGACTCCAACTCAC- CAATTATGG ACACCGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTCAGGCTTTGATTGGGTGACGGATCATTCTGGAAAAACA- GTTTGGTT TGTTCCAAGCGTGAGGAACGGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAACGGGTCATACAGCTCA- GCAGAAAG ACTTTTGAGACAGAGTTCCAGAAAACAAAACATCAAGAGTGGGACTTTGTCGTGACAACTGACATTTCAGAGAT- GGGCGCCAA CTTTAAAGCTGACCGTGTCATAGATTCCAGGAGATGCCTAAAGCCGGTCATACTTGATGGCGAGAGAGTCATTC- TGGCTGGAC CCATGCCTGTCACACATGCCAGCGCTGCCCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGGAGAT- GAGTACCT GTATGGAGGTGGGTGCGCAGAGACTGACGAAGACCATGCACACTGGCTTGAAGCAAGAATGCTCCTTGACAATA- TTTACCTCC AAGATGGCCTCATAGCCTCGCTCTATCGACCTGAGGCCGACAAAGTAGCAGCCATTGAGGGAGAGTTCAAGCTT- AGGACGGA GCAAAGGAAGACCTTTGTGGAACTCATGAAAAGAGGAGATCTTCCTGTTTGGCTGGCCTATCAGGTTGCATCTG- CCGGAATAA CCTACACAGATAGAAGATGGTGCTTTGATGGCACGACCAACAACACCATAATGGAAGACAGTGTGCCGGCAGAG- GTGTGGAC CAGACACGGAGAGAAAAGAGTGCTCAAACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCCTGA- AGTCATTC AAGGAGTTTGCCGCTGGGAAAAGAGGAGCGGCTTTTGGAGTGATGGAAGCCCTGGGAACACTGCCAGGACACAT- GACAGAG AGATTCCAGGAAGCCATTGACAACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGCCTTACAAAGCCGC- GGCGGCCC AATTGCCGGAGACCCTAGAGACCATTATGCTTTTGGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTTTTCGTC- TTGATGAGG AACAAGGGCATAGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGCGCATGGCTCATGTGGCTCTCGGA- AATTGAGC CAGCCAGAATTGCATGTGTCCTCATTGTTGTGTTCCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAA- AGATCTCCCC AGGACAACCAAATGGCAATCATCATCATGGTAGCAGTAGGTCTTCTGGGCTTGATTACCGCCAATGAACTCGGA- TGGTTGGAG AGAACAAAGAGTGACCTAAGCCATCTAATGGGAAGGAGAGAGGAGGGGGCAACCATAGGATTCTCAATGGACAT- TGACCTG CGGCCAGCCTCAGCTTGGGCCATCTATGCTGCCTTGACAACTTTCATTACCCCAGCCGTCCAACATGCAGTGAC- CACTTCATACA ACAACTACTCCTTAATGGCGATGGCCACGCAAGCTGGAGTGTTGTTTGGTATGGGCAAAGGGATGCCATTCTAC- GCATGGGAC TTTGGAGTCCCGCTGCTAATGATAGGTTGCTACTCACAATTAACACCCCTGACCCTAATAGTGGCCATCATTTT- GCTCGTGGCGC ACTACATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCGTGCTGCCCAGAAGAGAACGGCAGCTGGCATC- ATGAAGAA CCCTGTTGTGGATGGAATAGTGGTGACTGACATTGACACAATGACAATTGACCCCCAAGTGGAGAAAAAGATGG- GACAGGTG CTACTCATAGCAGTAGCCGTCTCCAGCGCCATACTGTCGCGGACCGCCTGGGGGTGGGGGGAGGCTGGGGCCCT- GATCACAG CCGCAACTTCCACTTTGTGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCTACAGCCACTTCACTGTGTAAC- ATTTTTAGGG GAAGTTACTTGGCTGGAGCTTCTCTAATCTACACAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGGT- GGAACAGG AGAGACCCTGGGAGAGAAATGGAAGGCCCGCTTGAACCAGATGTCGGCCCTGGAGTTCTACTCCTACAAAAAGT- CAGGCATC ACCGAGGTGTGCAGAGAAGAGGCCCGCCGCGCCCTCAAGGACGGTGTGGCAACGGGAGGCCATGCTGTGTCCCG- AGGAAGT GCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCTGCAGCCCTATGGAAAGGTCATTGATCTTGGATGTGGCAG- AGGGGGC TGGAGTTACTACGCCGCCACCATCCGCAAAGTTCAAGAAGTGAAAGGATACACAAAAGGAGGCCCTGGTCATGA- AGAACCCG TGTTGGTGCAAAGCTATGGGTGGAACATAGTCCGTCTTAAGAGTGGGGTGGACGTCTTTCATATGGCGGCTGAG- CCGTGTGA CACGTTGCTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAAGCACGGACGCTCAGAGTCCTCT- CCATGGTGG GGGATTGGCTTGAAAAAAGACCAGGAGCCTTTTGCATAAAAGTGTTGTGCCCATACACCAGCACTATGATGGAA- ACCCTGGAG CGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGAGTGCCACTCTCCCGCAACTCTACACATGAGATGTACTG- GGTCTCTG GAGCGAAAAGCAACACCATAAAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCGCATGGACGGGCCTAGG- AGGCCAGT GAAATATGAGGAGGATGTGAATCTCGGCTCTGGCACGCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACATGA- AGATCATT GGTAACCGCATTGAAAGGATCCGCAGTGAGCACGCGGAAACGTGGTTCTTTGACGAAAACCACCCATATAGGAC- ATGGGCTT ACCATGGAAGCTATGTGGCCCCCACACAAGGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGTCA- AAACCCTGG GATGTGGTGACTGGAGTCACAGGAATAGCCATGACCGACACCACACCGTATGGTCAGCAAAGAGTTTTCAAGGA- AAAAGTGG ACACTAGGGTGCCAGACCCCCAAGAAGGCACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTTGTGGAAAGAG- CTAGGCAA ACACAAACGACCACGAGTCTGTACCAAAGAAGAGTTCATCAACAAGGTTCGTAGCAATGCAGCATTAGGGGCAA- TATTTGAAG AGGAAAAAGAGTGGAAGACTGCAGTGGAAGCTGTGAACGATCCAAGGTTCTGGGCTCTAGTGGACAAGGAAAGA- GAGCACC ACCTGAGAGGAGAGTGCCAGAGTTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAACAAGGGGAATTTGGA- AAGGCCA AGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAGATTTCTAGAGTTCGAAGCCCTTGGATTCTTGAAC- GAGGATCAC TGGATGGGGAGAGAGAACTCAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGATATGTCCTAGAAGA- GATGAGT CGCATACCAGGAGGAAGGATGTATGCAGATGACACTGCTGGCTGGGACACCCGCATCAGCAGGTTTGATCTGGA- GAATGAAG CTCTAATCACCAACCAAATGGAGAAAGGGCACAGGGCCTTGGCATTGGCCATAATCAAGTACACATACCAAAAC- AAAGTGGTA AAGGTCCTTAGACCAGCTGAAAAAGGGAAAACAGTTATGGACATTATTTCGAGACAAGACCAAAGGGGGAGCGG- ACAAGTT GTCACTTACGCTCTTAACACATTTACCAACCTAGTGGTGCAACTCATTCGGAATATGGAGGCTGAGGAAGTTCT- AGAGATGCAA GACTTGTGGCTGCTGCGGAGGTCAGAGAAAGTGACCAACTGGTTGCAGAGCAACGGATGGGATAGGCTCAAACG- AATGGCA GTCAGTGGAGATGATTGCGTTGTGAAGCCAATTGATGATAGGTTTGCACATGCCCTCAGGTTCTTGAATGATAT- GGGAAAAGT TAGGAAGGACACACAAGAGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAAGTTCCGTTTTGCTCCCACC- ACTTCAACA AGCTCCATCTCAAGGACGGGAGGTCCATTGTGGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCCCGC-

GTCTCTCCA GGGGCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGCAAATGTGGCAGCTCCTTTATTT- CCACAGAA GGGACCTCCGACTGATGGCCAATGCCATTTGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGGAGAACTACC- TGGTCAATCC ATGGAAAGGGAGAATGGATGACCACTGAAGACATGCTTGTGGTGTGGAACAGAGTGTGGATTGAGGAGAACGAC- CACATGG AAGACAAGACCCCAGTTACGAAATGGACAGACATTCCCTATTTGGGAAAAAGGGAAGACTTGTGGTGTGGATCT- CTCATAGG GCACAGACCGCGCACCACCTGGGCTGAGAACATTAAAAATACAGTCAACATGGTGCGCAGGATCATAGGTGATG- AAGAAAAG TACATGGACTACCTATCCACCCAAGTTCGCTACTTGGGTGAAGAAGGGTCTACACCTGGAGTGCTGTGAGCACC- AATCTTAATG TTGTCAGGCCTGCTAGTCAGCCACAGCTTGGGGAAAGCTGTGCAGCCTGTGACCCCTCCAGGAGAAGCTGGGTA- ACCAAGCCT ATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTGAGCCCCTCAGAGGACACTGAGTCAA- AAAACCCC ACGCGCTTGGAGGCGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAACTGGA- GATCAGCT GTGGATCTCCAGAAGAGGGACTAGTGGTTAGAGGAGACCCCCCGGAAAACGCAAAACAGCATATTGACGCTGGG- AAAGACC AGAGACTCCATGAGTTTCCACCACGCTGGCCGCCAGGCACAGATCGCCGAATAGCGGCGGCCGGTGTGGGGAAA- TCCA KU501215.1 Zika virus strain PRVABC59, Puerto Rico, complete genome SEQ ID NO: 4 GTTGTTGATCTGTGTGAATCAGACTGCGACAGTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTT- TATTTTGGAT TTGGAAACGAGAGTTTCTGGTCATGAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATATGCTAA- AACGCGGA GTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGTCATGGGCCCATCAG- GATGGTCTT GGCGATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAGATGGGGTTCAGTGG- GGAAAAAAG AGGCTATGGAAACAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGCTGAGAATAATCAATGCTAGGAAGGAG- AAGAAGA GACGAGGCGCAGATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGCGGAGGTCACTAGA- CGTGGGAG TGCATACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATA- AGTGTTATAT ACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGGTGG- AACCAGAT GACGTCGATTGTTGGTGCAACACGACGTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAAAAGGTGAAGC- ACGGAGAT CTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACCAGGAAGCTGCAAACGCGGTCGCAAACCTGGTTGGAATCA- AGAGAATAC ACAAAGCACTTGATTAGAGTCGAAAATTGGATATTCAGGAACCCTGGCTTCGCGTTAGCAGCAGCTGCCATCGC- TTGGCTTTTG GGAAGCTCAACGAGCCAAAAAGTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTG- CATAGGAGT CAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTGATGTTGTCTTGGAACATGGAGGTTGTG- TCACCGTA ATGGCACAGGACAAACCGACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGTAAGATC- CTACTGCT ATGAGGCATCAATATCAGACATGGCTTCTGACAGCCGCTGCCCAACACAAGGTGAAGCCTACCTTGACAAGCAA- TCAGACACT CAATATGTCTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGCCT- GGTGACAT GCGCTAAGTTTGCATGCTCCAAGAAAATGACCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAATG- CTGTCAGT TCATGGCTCCCAGCACAGTGGGATGATCGTTAATGACACAGGACATGAAACTGATGAGAATAGAGCGAAAGTTG- AGATAACG CCCAATTCACCGAGAGCCGAAGCCACCCTGGGGGGTTTTGGAAGCCTAGGACTTGATTGTGAACCGAGGACAGG- CCTTGACTT TTCAGATTTGTATTACTTGACTATGAATAACAAGCACTGGTTGGTTCACAAGGAGTGGTTCCACGACATTCCAT- TACCTTGGCAC GCTGGGGCAGACACCGGAACTCCACACTGGAACAACAAAGAAGCACTGGTAGAGTTCAAGGACGCACATGCCAA- AAGGCAA ACTGTCGTGGTTCTAGGGAGTCAAGAAGGAGCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGGA- TGGTGCAA AGGGAAGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATAAACTTAGATTGAAGGGCGTGTCATAC- TCCTTGTGT ACTGCAGCGTTCACATTCACCAAGATCCCGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGTACAGTACGC- AGGGACAG ATGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGACATGCAAACTCTGACCCCAGTTGGGAGGTTGATAACC- GCTAACCCC GTAATCACTGAAAGCACTGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACATTGT- CATAGGAGTC GGGGAGAAGAAGATCACCCACCACTGGCACAGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGAG- AGGTGCC AAGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGTTGGAGGCGCTCTCAACTCATTGGGCAA- GGGCATCC ATCAAATTTTTGGAGCAGCTTTCAAATCATTGTTTGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGAACG- TTGCTGATGTG GTTGGGTCTGAACACAAAGAATGGATCTATTTCCCTTATGTGCTTGGCCTTAGGGGGAGTGTTGATCTTCTTAT- CCACAGCCGT CTCTGCTGATGTGGGGTGCTCGGTGGACTTCTCAAAGAAGGAGACGAGATGCGGTACAGGGGTGTTCGTCTATA- ACGACGTT GAAGCCTGGAGGGACAGGTACAAGTACCATCCTGACTCCCCCCGTAGATTGGCAGCAGCAGTCAAGCAAGCCTG- GGAAGATG GTATCTGCGGGATCTCCTCTGTTTCAAGAATGGAAAACATCATGTGGAGATCAGTAGAAGGGGAGCTCAACGCA- ATCCTGGAA GAGAATGGAGTTCAACTGACGGTCGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATTGCC- CGTGCCTG TGAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCGTATTTCGTCAGAGCAGCAAAGACAAATAACAGC- TTTGTCGT GGATGGTGACACACTGAAGGAATGCCCACTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAGGATCATGGGT- TCGGGGTA TTTCACACTAGTGTCTGGCTCAAGGTTAGAGAAGATTATTCATTAGAGTGTGATCCAGCCGTTATTGGAACAGC- TGTTAAGGGA AAGGAGGCTGTACACAGTGATCTAGGCTACTGGATTGAGAGTGAGAAGAATGACACATGGAGGCTGAAGAGGGC- CCATCTG ATCGAGATGAAAACATGTGAATGGCCAAAGTCCCACACATTGTGGACAGATGGAATAGAAGAGAGTGATCTGAT- CATACCCA AGTCTTTAGCTGGGCCACTCAGCCATCACAATACCAGAGAGGGCTACAGGACCCAAATGAAAGGGCCATGGCAC- AGTGAAGA GCTTGAAATTCGGTTTGAGGAATGCCCAGGCACTAAGGTCCACGTGGAGGAAACATGTGGAACAAGAGGACCAT- CTCTGAGA TCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGTGCTGCAGGGAGTGCACAATGCCCCCACTGTCGTTCCG- GGCTAAAG ATGGCTGTTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAAGCAACTTAGTAAGGTCAATGGTGACT- GCAGGATC AACTGATCACATGGACCACTTCTCCCTTGGAGTGCTTGTGATCCTGCTCATGGTGCAGGAAGGGCTGAAGAAGA- GAATGACCA CAAAGATCATCATAAGCACATCAATGGCAGTGCTGGTAGCTATGATCCTGGGAGGATTTTCAATGAGTGACCTG- GCTAAGCTT GCAATTTTGATGGGTGCCACCTTCGCGGAAATGAACACTGGAGGAGATGTAGCTCATCTGGCGCTGATAGCGGC- ATTCAAAGT CAGACCAGCGTTGCTGGTATCTTTCATCTTCAGAGCTAATTGGACACCCCGTGAAAGCATGCTGCTGGCCTTGG- CCTCGTGTCT TTTGCAAACTGCGATCTCCGCCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGGTTGG- CAATACGAGC GATGGTTGTTCCACGCACTGATAACATCACCTTGGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCACAC- TGCTTGTGGC GTGGAGAGCAGGCCTTGCTACTTGCGGGGGGTTTATGCTCCTCTCTCTGAAGGGAAAAGGCAGTGTGAAGAAGA- ACTTACCA TTTGTCATGGCCCTGGGACTAACCGCTGTGAGGCTGGTCGACCCCATCAACGTGGTGGGACTGCTGTTGCTCAC- AAGGAGTGG GAAGCGGAGCTGGCCCCCTAGCGAAGTACTCACAGCTGTTGGCCTGATATGCGCATTGGCTGGAGGGTTCGCCA- AGGCAGAT ATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAGGAAAGAGTGTGGA- CATGTACA TTGAAAGAGCAGGTGACATCACATGGGAAAAAGATGCGGAAGTCACTGGAAACAGTCCCCGGCTCGATGTGGCG- CTAGATGA GAGTGGTGATTTCTCCCTGGTGGAGGATGACGGTCCCCCCATGAGAGAGATCATACTCAAGGTGGTCCTGATGA- CCATCTGTG GCATGAACCCAATAGCCATACCCTTTGCAGCTGGAGCGTGGTACGTATACGTGAAGACTGGAAAAAGGAGTGGT- GCTCTATG GGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCACAGATGGAGTGTACAGAGTAATGACTCGTAGAC- TGCTAGG TTCAACACAAGTTGGAGTGGGAGTTATGCAAGAGGGGGTCTTTCACACTATGTGGCACGTCACAAAAGGATCCG- CGCTGAGA AGCGGTGAAGGGAGACTTGATCCATACTGGGGAGATGTCAAGCAGGATCTGGTGTCATACTGTGGTCCATGGAA- GCTAGATG CCGCCTGGGATGGGCACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGAGAGCGAGGAACATCCAGACT- CTGCCCG GAATATTTAAGACAAAGGATGGGGACATTGGAGCGGTTGCGCTGGATTACCCAGCAGGAACTTCAGGATCTCCA- ATCCTAGA CAAGTGTGGGAGAGTGATAGGACTTTATGGCAATGGGGTCGTGATCAAAAACGGGAGTTATGTTAGTGCCATCA- CCCAAGGG AGGAGGGAGGAAGAGACTCCTGTTGAGTGCTTCGAGCCCTCGATGCTGAAGAAGAAGCAGCTAACTGTCTTAGA- CTTGCATC CTGGAGCTGGGAAAACCAGGAGAGTTCTTCCTGAAATAGTCCGTGAAGCCATAAAAACAAGACTCCGTACTGTG- ATCTTAGCT CCAACCAGGGTTGTCGCTGCTGAAATGGAGGAGGCCCTTAGAGGGCTTCCAGTGCGTTATATGACAACAGCAGT- CAATGTCAC CCACTCTGGAACAGAAATCGTCGACTTAATGTGCCATGCCACCTTCACTTCACGTCTACTACAGCCAATCAGAG- TCCCCAACTAT AATCTGTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAAGAGGATACATTTCAACAAG- GGTTGAGAT GGGCGAGGCGGCTGCCATCTTCATGACCGCCACGCCACCAGGAACCCGTGACGCATTTCCGGACTCCAACTCAC- CAATTATGG ACACCGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTCAGGCTTTGATTGGGTGACGGATCATTCTGGAAAAACA- GTTTGGTT TGTTCCAAGCGTGAGGAACGGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAACGGGTCATACAGCTCA- GCAGAAAG ACTTTTGAGACAGAGTTCCAGAAAACAAAACATCAAGAGTGGGACTTTGTCGTGACAACTGACATTTCAGAGAT- GGGCGCCAA CTTTAAAGCTGACCGTGTCATAGATTCCAGGAGATGCCTAAAGCCGGTCATACTTGATGGCGAGAGAGTCATTC-

TGGCTGGAC CCATGCCTGTCACACATGCCAGCGCTGCCCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGGAGAT- GAGTATCT GTATGGAGGTGGGTGCGCAGAGACTGACGAAGACCATGCACACTGGCTTGAAGCAAGAATGCTCCTTGACAATA- TTTACCTCC AAGATGGCCTCATAGCCTCGCTCTATCGACCTGAGGCCGACAAAGTAGCAGCCATTGAGGGAGAGTTCAAGCTT- AGGACGGA GCAAAGGAAGACCTTTGTGGAACTCATGAAAAGAGGAGATCTTCCTGTTTGGCTGGCCTATCAGGTTGCATCTG- CCGGAATAA CCTACACAGATAGAAGATGGTGCTTTGATGGCACGACCAACAACACCATAATGGAAGACAGTGTGCCGGCAGAG- GTGTGGAC CAGACACGGAGAGAAAAGAGTGCTCAAACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCCTGA- AGTCATTC AAGGAGTTTGCCGCTGGGAAAAGAGGAGCGGCTTTTGGAGTGATGGAAGCCCTGGGAACACTGCCAGGACACAT- GACAGAG AGATTCCAGGAAGCCATTGACAACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGCCTTACAAAGCCGC- GGCGGCCC AATTGCCGGAGACCCTAGAGACCATAATGCTTTTGGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTCTTCGTC- TTGATGAGG AACAAGGGCATAGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGCGCATGGCTCATGTGGCTCTCGGA- AATTGAGC CAGCCAGAATTGCATGTGTCCTCATTGTTGTGTTCCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAA- AGATCTCCCC AGGACAACCAAATGGCAATCATCATCATGGTAGCAGTAGGTCTTCTGGGCTTGATTACCGCCAATGAACTCGGA- TGGTTGGAG AGAACAAAGAGTGACCTAAGCCATCTAATGGGAAGGAGAGAGGAGGGGGCAACCATAGGATTCTCAATGGACAT- TGACCTG CGGCCAGCCTCAGCTTGGGCCATCTATGCTGCCTTGACAACTTTCATTACCCCAGCCGTCCAACATGCAGTGAC- CACCTCATACA ACAACTACTCCTTAATGGCGATGGCCACGCAAGCTGGAGTGTTGTTTGGCATGGGCAAAGGGATGCCATTCTAC- GCATGGGAC TTTGGAGTCCCGCTGCTAATGATAGGTTGCTACTCACAATTAACACCCCTGACCCTAATAGTGGCCATCATTTT- GCTCGTGGCGC ACTACATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCGTGCTGCCCAGAAGAGAACGGCAGCTGGCATC- ATGAAGAA CCCTGTTGTGGATGGAATAGTGGTGACTGACATTGACACAATGACAATTGACCCCCAAGTGGAGAAAAAGATGG- GACAGGTG CTACTCATAGCAGTAGCCGTCTCCAGCGCCATACTGTCGCGGACCGCCTGGGGGTGGGGGGAGGCTGGGGCTCT- GATCACAG CCGCAACTTCCACTTTGTGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCTACAGCCACTTCACTGTGTAAC- ATTTTTAGGG GAAGTTACTTGGCTGGAGCTTCTCTAATCTACACAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGGT- GGAACAGG AGAGACCCTGGGAGAGAAATGGAAGGCCCGCTTGAACCAGATGTCGGCCCTGGAGTTCTACTCCTACAAAAAGT- CAGGCATC ACCGAGGTGTGCAGAGAAGAGGCCCGCCGCGCCCTCAAGGACGGTGTGGCAACGGGAGGCCATGCTGTGTCCCG- AGGAAGT GCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCTGCAGCCCTATGGAAAGGTCATTGATCTTGGATGTGGCAG- AGGGGGC TGGAGTTACTACGTCGCCACCATCCGCAAAGTTCAAGAAGTGAAAGGATACACAAAAGGAGGCCCTGGTCATGA- AGAACCCG TGTTGGTGCAAAGCTATGGGTGGAACATAGTCCGTCTTAAGAGTGGGGTGGACGTCTTTCATATGGCGGCTGAG- CCGTGTGA CACGTTGCTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAAGCACGGACGCTCAGAGTCCTCT- CCATGGTGG GGGATTGGCTTGAAAAAAGACCAGGAGCCTTTTGTATAAAAGTGTTGTGCCCATACACCAGCACTATGATGGAA- ACCCTGGAG CGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGAGTGCCACTCTCCCGCAACTCTACACATGAGATGTACTG- GGTCTCTG GAGCGAAAAGCAACACCATAAAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCGCATGGACGGGCCTAGG- AGGCCAGT GAAATATGAGGAGGATGTGAATCTCGGCTCTGGCACGCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACATGA- AGATCATT GGTAACCGCATTGAAAGGATCCGCAGTGAGCACGCGGAAACGTGGTTCTTTGACGAGAACCACCCATATAGGAC- ATGGGCTT ACCATGGAAGCTATGAGGCCCCCACACAAGGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGTCA- AAACCCTGG GATGTGGTGACTGGAGTCACAGGAATAGCCATGACCGACACCACACCGTATGGTCAGCAAAGAGTTTTCAAGGA- AAAAGTGG ACACTAGGGTGCCAGACCCCCAAGAAGGCACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTTGTGGAAAGAG- CTAGGCAA ACACAAACGGCCACGAGTCTGCACCAAAGAAGAGTTCATCAACAAGGTTCGTAGCAATGCAGCATTAGGGGCAA- TATTTGAA GAGGAAAAAGAGTGGAAGACTGCAGTGGAAGCTGTGAACGATCCAAGGTTCTGGGCTCTAGTGGACAAGGAAAG- AGAGCAC CACCTGAGAGGAGAGTGCCAGAGCTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAACAAGGGGAATTTGG- AAAGGCC AAGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAGATTTCTAGAGTTCGAAGCCCTTGGATTCTTGAA- CGAGGATCA CTGGATGGGGAGAGAGAACTCAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGATATGTCCTAGAAG- AGATGAG TCGTATACCAGGAGGAAGGATGTATGCAGATGACACTGCTGGCTGGGACACCCGCATTAGCAGGTTTGATCTGG- AGAATGAA GCTCTAATCACCAACCAAATGGAGAAAGGGCACAGGGCCTTGGCATTGGCCATAATCAAGTACACATACCAAAA- CAAAGTGGT AAAGGTCCTTAGACCAGCTGAAAAAGGGAAAACAGTTATGGACATTATTTCGAGACAAGACCAAAGGGGGAGCG- GACAAGTT GTCACTTACGCTCTTAACACATTTACCAACCTAGTGGTGCAACTCATTCGGAATATGGAGGCTGAGGAAGTTCT- AGAGATGCAA GACTTGTGGCTGCTGCGGAGGTCAGAGAAAGTGACCAACTGGTTGCAGAGCAACGGATGGGATAGGCTCAAACG- AATGGCA GTCAGTGGAGATGATTGCGTTGTGAAGCCAATTGATGATAGGTTTGCACATGCCCTCAGGTTCTTGAATGATAT- GGGAAAAGT TAGGAAGGACACACAAGAGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAAGTTCCGTTTTGCTCCCACC- ACTTCAACA AGCTCCATCTCAAGGACGGGAGGTCCATTGTGGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCCCGC- GTCTCTCCA GGGGCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGCAAATGTGGCAGCTCCTTTATTT- CCACAGAA GGGACCTCCGACTGATGGCCAATGCCATTTGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGGAGAACTACC- TGGTCAATCC ATGGAAAGGGAGAATGGATGACCACTGAAGACATGCTTGTGGTGTGGAACAGAGTGTGGATTGAGGAGAACGAC- CACATGG AAGACAAGACCCCAGTTACGAAATGGACAGACATTCCCTATTTGGGAAAAAGGGAAGACTTGTGGTGTGGATCT- CTCATAGG GCACAGACCGCGCACCACCTGGGCTGAGAACATTAAAAACACAGTCAACATGGTGCGCAGGATCATAGGTGATG- AAGAAAAG TACATGGACTACCTATCCACCCAAGTTCGCTACTTGGGTGAAGAAGGGTCTACACCTGGAGTGCTGTAAGCACC- AATCTTAATG TTGTCAGGCCTGCTAGTCAGCCACAGCTTGGGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGAAGCTGGGAA- ACCAAGCC TATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTGAGCCCCTCAGAGGACACTGAGTCA- AAAAACCC CACGCGCTTGGAGGCGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAACTGG- AGATCAGC TGTGGATCTCCAGAAGAGGGACTAGTGGTTAGAGGA KU509998.1 Zika virus strain Haiti/1225/2014, Haiti, complete genome SEQ ID NO: 5 GTTGTTACTGTTGCTGACTCAGACTGCGACAGTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTT- TATTTGGATT TGGAAACGAGAGTTTCTGGTCATGAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATATGCTAAA- ACGCGGAG TAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGTCATGGGCCCATCAGG- ATGGTCTTG GCAATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAGATGGGGTTCAGTGGG- GAAAAAAGA GGCTATGGAAATAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGCTGAGAATAATCAATGCTAGGAAGGAGA- AGAAGAG ACGAGGCGCAGATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGCGGAGGTCACTAGAC- GTGGGAGT GCATACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAA- GTGTTATAT ACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGGTGG- AACCAGAT GACGTCGATTGTTGGTGCAACACGACGTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAAAAGGTGAAGC- ACGGAGAT CTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACTAGGAAGCTGCAAACGCGGTCGCAAACCTGGTTGGAATCA- AGAGAATAC ACAAAGCACTTGATTAGAGTCGAAAATTGGATATTCAGGAACCCTGGCTTCGCGTTAGCAGCAGCTGCCATCGC- TTGGCTTTTG GGAAGCTCAACGAGCCAAAAAGTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTG- CATAGGAGT CAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTGATGTTGTCTTGGAACATGGAGGTTGTG- TCACCGTA ATGGCACAGGACAAACCGACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGTAAGATC- CTACTGCT ATGAGGCATCAATATCAGACATGGCTTCGGACAGCCGCTGCCCAACACAAGGTGAAGCCTACCTTGACAAGCAA- TCAGACACT CAATATGTCTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGTCT- GGTGACAT GCGCTAAGTTTGCATGCTCCAAGAAAATGACCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAATG- CTGTCAGT TCATGGCTCCCAGCACAGTGGGATGATCGTTAATGACACAGGACATGAAACTGATGAGAATAGAGCGAAGGTTG- AGATAACG CCCAATTCACCAAGAGCCGAAGCCACCCTGGGGGGTTTTGGAAGCCTAGGACTTGATTGTGAACCGAGGACAGG- CCTTGACTT TTCAGATTTGTATTACTTGACTATGAATAACAAGCACTGGTTGGTTCACAAGGAGTGGTTCCACGACATTCCAT- TACCTTGGCAC GCTGGGGCAGACACCGGAACTCCACACTGGAACAACAAAGAAGCACTGGTAGAGTTCAAGGACGCACATGCCAA- AAGGCAA ACTGTCGTGGTTCTAGGGAGTCAAGAAGGAGCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGGA- TGGTGCAA AGGGAAGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATAAACTTAGATTGAAGGGCGTGTCATAC- TCCTTGTGT ACCGCAGCGTTCACATTCACCAAGATCCCGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGTACAGTACGC- AGGGACAG ATGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGACATGCAAACTCTGACCCCAGTTGGGAGGTTGATAACC- GCTAACCCC

GTAATCACTGAAAGCACTGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACATTGT- CATAGGAGTC GGGGAGAAGAAGATCACCCACCACTGGCACAGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGAG- AGGTGCC AAGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGTTGGAGGCGCTCTCAACTCATTGGGCAA- GGGCATCC ATCAAATTTTTGGAGCAGCTTTCAAATCATTGTTTGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGAACG- TTGCTGATGTG GTTGGGTCTGAACACAAAGAATGGATCTATTTCCCTTATGTGCTTGGCCTTAGGGGGAGTGTTGATCTTCTTAT- CCACAGCCGT CTCTGCTGATGTGGGGTGCTCGGTGGACTTCTCAAAGAAGGAGACGAGATGCGGTACAGGGGTGTTCGTCTATA- ACGACGTT GAAGCCTGGAGGGACAGGTACAAGTACCATCCTGACTCCCCCCGTAGATTGGCAGCAGCAGTCAAGCAAGCCTG- GGAAGATG GTATCTGCGGGATCTCCTCTGTTTCAAGAATGGAAAACATCATGTGGAGATCAGTAGAAGGGGAGCTCAACGCA- ATCCTGGAA GAGAATGGAGTTCAACTGACGGTCGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATTGCC- CGTGCCTG TGAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCGCACTTCGTCAGAGCAGCAAAGACAAATAACAGC- TTTGTCGT GGATGGTGACACACTGAAGGAATGCCCACTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAGGATCATGGGT- TCGGGGTA TTTCACACTAGTGTCTGGCTCAAGGTTAGAGAAGATTATTCATTAGAGTGTGATCCAGCCGTTATTGGAACAGC- TGTTAAGGGA AAGGAGGCTGTACACAGTGATCTAGGCTACTGGATTGAGAGTGAGAAGAATGACACATGGAGGCTGAAGAGGGC- CCATCTG ATCGAGATGAAAACATGTGAATGGCCAAAGTCCCACACATTGTGGACAGATGGAATAGAAGAGAGTGATCTGAT- CATACCCA AGTCTTTAGCTGGGCCACTCAGCCATCACAATACCAGAGAGGGCTACAGGACCCAAATGAAAGGGCCATGGCAC- AGTGAAGA GCTTGAAATTCGGTTTGAGGAATGCCCAGGCACTAAGGTCCACGTGGAGGAAACATGTGGAACAAGAGGACCAT- CTCTGAGA TCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGTGCTGCAGGGAGTGCACAATGCCCCCACTGTCGTTCCG- GGCTAAAG ATGGCTGTTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAAGCAACTTAGTAAGGTCAATGGTGACT- GCAGGATC AACTGATCACATGGATCACTTCTCCCTTGGAGTGCTTGTGATTCTGCTCATGGTGCAGGAAGGGCTGAAGAAGA- GAATGACCA CAAAGATCATCATAAGCACATCAATGGCAGTGCTGGTAGCTATGATCCTGGGAGGATTTTCAATGAGTGACCTG- GCTAAGCTT GCAATTTTGATGGGTGCCACCTTCGCGGAAATGAACACTGGAGGAGATGTAGCTCATCTGGCGCTGATAGCGGC- ATTCAAAGT CAGACCAGCGTTGCTGGTATCTTTCATCTTCAGAGCTAATTGGACACCCCGTGAAAGCATGCTGCTGGCCTTGG- CCTCGTGTCT TTTGCAAACTGCGATCTCCGCCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGGTTGG- CAATACGAGC GATGGTTGTTCCACGCACTGATAACATCACCTTGGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCACAC- TGCTTGTGGC GTGGAGAGCAGGCCTTGCTACTTGCGGGGGGTTTATGCTCCTCTCTCTGAAGGGAAAAGGCAGTGTGAAGAAGA- ACTTACCA TTTGTCATGGCCCTGGGACTAACCGCTGTGAGGCTGGTCGACCCCATCAACGTGGTGGGGCTGCTGTTGCTCAC- AAGGAGTGG GAAGCGGAGCTGGCCCCCTAGCGAAGTACTCACAGCTGTTGGCCTGATATGCGCATTGGCTGGAGGGTTCGCCA- AGGCAGAT ATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAGGAAAGAGTGTGGA- CATGTACA TTGAAAGAGCAGGTGACATCACATGGGAAAAAGATGCGGAAGTCACTGGAAACAGTCCCCGGCTCGATGTGGCG- CTAGATGA GAGTGGTGATTTCTCCCTGGTGGAGGATGACGGTCCCCCCATGAGAGAGATCATACTCAAGGTGGTCCTGATGA- CCATCTGTG GCATGAACCCAATAGCCATACCCTTTGCAGCTGGAGCGTGGTACGTATACGTGAAGACTGGAAAAAGGAGTGGT- GCTCTATG GGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCACAGATGGAGTGTACAGAGTAATGACTCGTAGAC- TGCTAGG TTCAACACAAGTTGGAGTGGGAGTTATGCAAGAGGGGGTCTTTCACACTATGTGGCACGTCACAAAAGGATCCG- CGCTGAGA AGCGGTGAAGGGAGACTTGATCCATACTGGGGAGATGTCAAGCAGGATCTGGTGTCATACTGTGGTCCATGGAA- GCTAGATG CCGCCTGGGACGGGCACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGAGAGCGAGGAACATCCAGACT- CTGCCCG GAATATTTAAGACAAAGGATGGGGACATTGGAGCGGTTGCGCTGGATTACCCAGCAGGAACTTCAGGATCTCCA- ATCCTAGA CAAGTGTGGGAGAGTGATAGGACTTTATGGCAATGGGGTCGTGATCAAAAATGGGAGTTATGTTAGTGCCATCA- CCCAAGGG AGGAGGGAGGAAGAGACTCCTGTTGAGTGCTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTAACTGTCTTAGA- CTTGCATC CTGGAGCTGGGAAAACCAGGAGAGTTCTTCCTGAAATAGTCCGTGAAGCCATAAAAACAAGACTCCGTACTGTG- ATCTTAGCT CCAACCAGGGTTGTCGCTGCTGAAATGGAGGAAGCCCTTAGAGGGCTTCCAGTGCGTTATATGACAACAGCAGT- CAATGTCAC CCACTCTGGAACAGAAATCGTCGACTTAATGTGCCATGCCACCTTCACTTCACGTCTACTACAGCCAATCAGAG- TCCCCAACTAT AATCTGTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAAGAGGATACATTTCAACAAG- GGTTGAGAT GGGCGAGGCGGCTGCCATCTTCATGACCGCCACGCCACCAGGAACCCGTGACGCATTTCCGGACTCCAACTCAC- CAATTATGG ACACCGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTCAGGCTTTGATTGGGTGACGGATTATTCTGGAAAAACA- GTTTGGTT TGTTCCAAGCGTGAGGAACGGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAACGGGTCATACAGCTCA- GCAGAAAG ACTTTTGAGACAGAGTTCCAGAAAACAAAACATCAAGAGTGGGACTTTGTCGTGACAACTGACATTTCAGAGAT- GGGCGCCAA CTTTAAAGCTGACCGTGTCATAGATTCCAGGAGATGCCTAAAGCCGGTCATACTTGATGGCGAGAGAGTCATTC- TGGCTGGAC CCATGCCTGTCACACATGCCAGCGCTGCCCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGGAGAT- GAGTATCT GTATGGAGGTGGGTGCGCAGAGACTGACGAAGACCATGCACACTGGCTTGAAGCAAGAATGCTCCTTGACAATA- TTTACCTCC AAGATGGCCTCATAGCCTCGCTCTATCGACCTGAGGCCGACAAAGTAGCAGCCATTGAGGGAGAGTTCAAGCTT- AGGACGGA GCAAAGGAAGACCTTTGTGGAACTCATGAAAAGAGGAGATCTTCCTGTTTGGCTGGCCTATCAGGTTGCATCTG- CCGGAATAA CCTACACAGATAGAAGATGGTGCTTTGATGGCACGACCAACAACACCATAATGGAAGACAGTGTGCCGGCAGAG- GTGTGGAC CAGACACGGAGAGAAAAGAGTGCTCAAACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCCTGA- AGTCATTC AAGGAGTTTGCCGCTGGGAAAAGAGGAGCGGCTTTTGGAGTGATGGAAGCCCTGGGAACACTGCCAGGACACAT- GACAGAG AGATTCCAGGAAGCCATTGACAACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGCCTTACAAAGCCGC- GGCGGCCC AATTGCCGGAGACCCTAGAGACCATTATGCTTTTGGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTTTTCGTC- TTGATGAGG AACAAGGGCATAGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGCGCATGGCTCATGTGGCTCTCGGA- AATTGAGC CAGCCAGAATTGCATGTGTCCTCATTGTTGTGTTCCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAA- AGATCTCCCC AGGACAACCAAATGGCAATCATCATCATGGTAGCAGTAGGTCTTCTGGGCTTGATTACCGCCAATGAACTCGGA- TGGTTGGAG AGAACAAAGAGTGACCTAAGCCATCTAATGGGAAGGAGAGAGGAGGGGGCAACCATGGGATTCTCAATGGACAT- TGACCTG CGGCCAGCCTCAGCTTGGGCCATCTATGCTGCCTTGACAACTTTCATTACCCCAGCCGTCCAACATGCAGTGAC- CACTTCATACA ACAACTACTCCTTAATGGCGATGGCCACGCAAGCTGGAGTGTTGTTTGGTATGGGCAAAGGGATGCCATTCTAC- GCATGGGAC TTTGGAGTCCCGCTGCTAATGATAGGTTGCTACTCACAATTAACGCCCCTGACCCTAATAGTGGCCATCATTTT- GCTCGTGGCG CACTACATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCGTGCTGCCCAGAAGAGAACGGCAGCTGGCAT- CATGAAGA ACCCTGTTGTGGATGGAATAGTGGTGACTGACATTGACACAATGACAATTGACCCCCAAGTGGAGAAAAAGATG- GGACAGGT GCTACTCATGGCAGTAGCCGTCTCCAGCGCCATACTGTCGCGGACCGCCTGGGGGTGGGGGGAGGCTGGGGCCC- TGATCACA GCCGCAACTTCCACTTTGTGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCTACAGCCACTTCACTGTGTAA- CATTTTTAGG GGAAGTTACTTGGCTGGAGCTTCTCTAATCTACACAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGG- TGGAACAG GAGAGACCCTGGGAGAGAAATGGAAGGCCCGCTTGAACCAGATGTCGGCCCTGGAGTTCTACTCCTACAAAAAG- TCAGGCAT CACCGAGGTGTGCAGAGAAGAGGCCCGCCGCGCCCTCAAGGACGGTGTGGCAACGGGAGGCCATGCTGTGTCCC- GAGGAAG TGCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCTGCAGCCCTATGGAAAGGTCATTGATCTTGGATGTGGCA- GAGGGGG CTGGAGTTACTACGCCGCCACCATCCGCAAAGTTCAAGAAGTGAAAGGATACACAAAAGGAGGCCCTGGTCATG- AAGAACCC GTGTTGGTGCAAAGCTATGGGTGGAACATAGTCCGTCTTAAGAGTGGGGTGGACGTCTTTCATATGGCGGCTGA- GCCGTGTG ACACGTTGCTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAAGCACGGACGCTCAGAGTCCTC- TCCATGGTG GGGGATTGGCTTGAAAAAAGACCAGGAGCCTTTTGTATAAAAGTGTTGTGCCCATACACCAGCACTATGATGGA- AACCCTGGA GCGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGAGTGCCACTCTCCCGCAACTCTACACATGAGATGTACT- GGGTCTCT GGAGCGAAAAGCAACACCATAAAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCGCATGGACGGGCCTAG- GAGGCCA GTGAAATATGAGGAGGATGTGAATCTCGGCTCTGGCACGCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACAT- GAAGATCA TTGGTAACCGCATTGAAAGGATCCGCAGTGAGCACGCGGAAACGTGGTTCTTTGACGAGAACCACCCATATAGG- ACATGGGC TTACCATGGAAGCTATGAGGCCCCCACACAAGGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGT- CAAAACCCT GGGATGTGGTGACTGGAGTCACAGGAATAGCCATGACCGACACCACACCGTATGGTCAGCAAAGAGTTTTCAAG- GAAAAAGT GGACACTAGGGTGCCAGACCCCCAAGAAGGCACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTTGTGGAAAG- AGCTAGGC AAACACAAACGGCCACGAGTCTGTACCAAAGAAGAGTTCATCAACAAGGTTCGTAGCAATGCAGCATTAGGGGC- AATATTTGA AGAGGAAAAAGAGTGGAAGACTGCAGTGGAAGCTGTGAACGATCCAAGGTTCTGGGCTCTAGTGGACAAGGAAA-

GAGAGCA CCACCTGAGAGGAGAGTGCCAGAGTTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAACAAGGGGAATTTG- GAAAGGC CAAGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAGATTTCTAGAGTTCGAAGCCCTTGGATTCTTGA- ACGAGGATC ACTGGATGGGGAGAGAGAACTCAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGATATGTCCTAGAA- GAGATGA GTCGCATACCAGGAGGAAGGATGTATGCAGATGACACTGCTGGCTGGGACACCCGCATCAGCAGGTTTGATCTG- GAGAATGA AGCTCTAATCACCAACCAAATGGAGAAAGGGCACAGGGCCTTGGCATTGGCCATAATCAAGTACACATACCAAA- ACAAAGTG GTAAAGGTCCTTAGACCAGCTGAAAAAGGGAAGACAGTTATGGACATTATTTCGAGACAAGACCAAAGGGGGAG- CGGACAA GTTGTCACTTACGCTCTTAACACATTTACCAACCTAGTGGTGCAACTCATTCGGAATATGGAGGCTGAGGAAGT- TCTAGAGATG CAAGACTTGTGGCTGCTGCGGAGGTCAGAGAAAGTGACCAACTGGTTGCAGAGCAACGGATGGGATAGGCTCAA- ACGAATG GCAGTCAGTGGAGATGATTGCGTTGTGAAGCCAATTGATGATAGGTTTGCACATGCCCTCAGGTTCTTGAATGA- TATGGGAAA AGTTAGGAAGGACACACAAGAGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAAGTTCCGTTTTGCTCCC- ACCACTTCA ACAAGCTCCATCTCAAGGACGGGAGGTCCATTGTGGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCC- CGCGTCTCT CCAGGGGCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGCAAATGTGGCAGCTCCTTTA- TTTCCACAG AAGGGACCTCCGACTGATGGCCAATGCCATTTGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGGAGAACTA- CCTGGTCAAT CCATGGAAAGGGAGAATGGATGACCACTGAAGACATGCTTGTGGTGTGGAACAGAGTGTGGATTGAGGAGAACG- ACCACAT GGAAGACAAGACCCCAGTTACGAAATGGACAGACATTCCCTATTTGGGAAAAAGGGAAGACTTGTGGTGTGGAT- CTCTCATA GGGCACAGACCGCGCACCACCTGGGCTGAGAACATTAAAAACACAGTCAACATGGTGCGCAGGATCATAGGTGA- TGAAGAAA AGTACATGGACTACCTATCCACCCAAGTTCGCTACTTGGGTGAAGAAGGGTCTACACCTGGAGTGCTGTAAGCA- CCAATCTTA ATGTTGTCAGGCCTGCTAGTCAGCCACAGCTTGGGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGAAGCTGG- GAAACCAA GCCTATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTGAGCCCCTCAGAGGACACTGAG- TCAAAAAA CCCCACGCGCTTGGAGGCGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAAC- TGGAGATC AGCTGTGGATCTCCAGAAGAGGGACTAGTGGTTAGAGGAGA KU527068.1 Zika virus strain Natal RGN, Brazil: Rio Grande do Norte, Natal, complete genome SEQ ID NO: 6 AGTTGTTGATCTGTGTGAATCAGACTGCGACAGTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTT- TTATTTTGG ATTTGGAAACGAGAGTTTCTGGTCATGAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATATGCT- AAAACGCGG AGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGTCATGGGCCCATCA- GGATGGTC TTGGCAATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAGATGGGGTTCAGT- GGGGAAAAA AGAGGCTATGGAAATAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGCTGAGAATAATCAATGCTAGGAAGG- AGAAGAA GAGACGAGGCGCAGATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGCGGAGGTCACTA- GACGTGGG AGTGCATACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAA- TAAGTGTTA TATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGG- TGGAACCAG ATGACGTCGATTGTTGGTGCAACACGACGTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAAAAGGTGAA- GCACGGAG ATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACTAGGAAGCTGCAAACGCGGTCGCAAACCTGGTTGGAAT- CAAGAGAAT ACACAAAGCACTTGATTAGAGTCGAAAATTGGATATTCAGGAACCCTGGCTTCGCGTTAGCAGCAGCTGCCATC- GCTTGGCTTT TGGGAAGCTCAACGAGCCAAAAAGTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGG- TGCATAGGA GTCAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTGATGTTGTCTTGGAACATGGAGGTTG- TGTCACCG TAATGGCACAGGACAAACCGACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGTAAGA- TCCTACTG CTATGAGGCATCAATATCAGACATGGCTTCGGACAGCCGCTGCCCAACACAAGGTGAAGCCTACCTTGACAAGC- AATCAGACA CTCAATATGTCTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGC- CTGGTGAC ATGCGCTAAGTTTGCATGCTCCAAGAAAATGACCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAA- TGCTGTCA GTTCATGGCTCCCAGCACAGTGGGATGATCGTTAATGACACAGGACATGAAACTGATGAGAATAGAGCGAAGGT- TGAGATAA CGCCCAATTCACCAAGAGCCGAAGCCACCCTGGGGGGTTTTGGAAGCCTAGGACTTGATTGTGAACCGAGGACA- GGCCTTGA CTTTTCAGATTTGTATTACTTGACTATGAATAACAAGCACTGGTTGGTCCACAAGGAGTGGTTCCACGACATTC- CATTACCTTGG CACGCTGGGGCAGACACCGGAACTCCACACTGGAACAACAAAGAAGCACTGGTAGAGTTCAAGGACGCACATGC- CAAAAGG CAAACTGTCGTGGTTCTAGGGAGTCAAGAAGGAGCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGAT- GGATGGTG CAAAGGGAAGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATAAACTTAGATTGAAGGGCGTGTCA- TACTCCTTG TGTACCGCAGCGTTCACATTCACCAAGATCCCGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGTACAGTA- CGCAGGGA CAGATGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGACATGCAAACTCTGACCCCAGTTGGGAGGTTGATA- ACCGCTAAC CCCGTAATCACTGAAAGCACTGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACAT- TGTCATAGGA GTCGGGGAGAAGAAGATCACCCACCACTGGCACAGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGT- GAGAGGT GCCAAGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGTTGGAGGCGCTCTCAACTCATTGGG- CAAGGGCA TCCATCAAATTTTTGGAGCAGCTTTCAAATCATTGTTTGGAGGAATGTCCTGGTTCTCACAAATCCTCATTGGA- ACGTTGCTGAT GTGGTTGGGTCTGAACACAAAGAATGGATCTATTTCCCTTATGTGCTTGGCCTTAGGGGGAGTGTTGATCTTCT- TATCCACAGC CGTCTCTGCTGATGTGGGGTGCTCGGTGGACTTCTCAAAGAAGGAGACGAGATGCGGTACAGGGGTGTTCGTCT- ATAACGAC GTTGAAGCCTGGAGGGACAGGTACAAGTACCATCCTGACTCCCCCCGTAGATTGGCAGCAGCAGTCAAGCAAGC- CTGGGAAG ATGGTATCTGCGGGATCTCCTCTGTTTCAAGAATGGAGAACATCATGTGGAGATCAGTAGAAGGGGAGCTCAAC- GCAATCTTG GAAGAGAATGGAGTTCAACTGACGGTCGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATT- GCCCGTGC CTGTGAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCGTACTTCGTCAGAGCAGCAAAGACAAATAAC- AGCTTTGT CGTGGATGGTGACACACTGAAGGAATGCCCACTCGAACATAGAGCATGGAACAGCTTTCTTGTGGAGGATCATG- GGTTCGGG GTATTTCACACTAGTGTCTGGCTCAAGGTTAGAGAAGATTATTCATTAGAGTGTGATCCAGCCGTTATTGGAAC- AGCTGTTAAG GGGAAGGAGGCTGTACACAGTGATCTAGGCTACTGGATTGAGAGTGAGAAGAATGACACATGGAGGCTGAAGAG- GGCCCAT CTAATCGAGATGAAAACATGTGAATGGCCAAAGTCCCACACATTGTGGGCAGATGGAATAGAAGAGAGTGATCT- GATCATTCC CAAGTCTTTAGCTGGGCCACTCAGCCATCACAATACCAGAGAGGGCTACAGGACCCAAATGAAAGGGCCATGGC- ACAGTGAA GAGCTTGAAATTCGGTTTGAGGAATGCCCGGGCACTAAGGTCCACGTGGAGGAAACATGTGGAACAAGAGGACC- ATCTCTGA GATCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGTGCTGCAGGGAGTGCACAATGCCCCCACTGTCGTTC- CGGGCTAA AGATGGCTGTTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAAGCAACTTAGTAAGGTCAGTGGTGA- CTGCAGG ATCAACTGATCACATGGATCACTTCTCCCTTGGAGTGCTTGTGATTCTGCTCATGGTGCAGGAAGGGCTGAAGA- AGAGAATGA CCACAAAGATCATCATAAGCACATCAATGGCAGTGCTGGTAGCTATGATCCTGGGAGGATTTTCAATGAGTGAC- CTGGCTAAG CTTGCAATTTTGATGGGCGCCACCTTCGCGGAAATGAACACTGGAGGAGATGTAGCTCATCTGGCGCTGATAGC- GGCATTCAA AGTCAGACCAGCGTTGCTGGTATCTTTCATCTTCAGAGCTAATTGGACACCCCGTGAAAGCATGCTGCTGGCCT- TGGCCTCGTG TCTTTTGCAAACTGCGATCTCCGCCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGGT- TGGCAATACG AGCGATGGTTGTTCCACGCACTGATAACATCACCTTGGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCA- CACTGCTTGT GGCGTGGAGAGCAGGCCTTGCTACTTGCGGGGGGTTTATGCTCCTCTCTCTGAAGGGAAAAGGCAGTGTGAAGA- AGAACTTA CCATTTGTCATGGCCCTGGGACTAACCGCTGTGAGGCTGGTCGACCCCATCAACGTGGTGGGACTGCTGTTGCT- CACAAGGAG TGGGAAGCGGAGCTGGCCCCCTAGCGAAGTACTCACAGCTGTTGGCCTGATATGCGCATTGGCTGGAGGGTTCG- CCAAGGCA GATATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAGGAAAGAGTGT- GGACATGT ACATTGAAAGAGCAGGTGACATCACATGGGAAAAAGATGCGGAAGTCACTGGAAACAGTCCCCGGCTCGATGTG- GCGCTAGA TGAGAGTGGTGATTTCTCCCTGGTGGAGGATGACGGTCCCCCCATGAGAGAGATCATACTCAAGGTGGTCCTGA- TGACCATCT GTGGCATGAACCCAATAGCCATACCCTTTGCAGCTGGAGCGTGGTACGTATACGTGAAGACTGGAAAAAGGAGT- GGTGCTCT ATGGGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCACAGATGGAGTGTACAGAGTAATGACTCGTA- GACTGCTA GGTTCAACACAAGTTGGAGTGGGAGTTATGCAAGAGGGGGTCTTTCACACTATGTGGCACGTCACAAAAGGATC- CGCGCTGA GAAGCGGTGAAGGGAGACTTGATCCATACTGGGGAGATGTCAAGCAGGATCTGGTGTCATACTGTGGTCCATGG- AAGCTAGA TGCCGCCTGGGACGGGCACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGAGAGCGAGGAACATCCAGA- CTCTGCCC GGAATATTTAAGACAAAGGATGGGGACATTGGAGCGGTTGCGCTGGATTACCCAGCAGGAACTTCAGGATCTCC- AATCCTAG ACAAGTGTGGGAGAGTGATAGGACTTTATGGCAATGGGGTCGTGATCAAAAATGGGAGTTATGTTAGTGCCATC-

ACCCAAGG GAGGAGGGAGGAAGAGACTCCTGTTGAGTGCTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTAACTGTCTTAG- ACTTGCAT CCTGGAGCTGGGAAAACCAGGAGAGTTCTTCCTGAAATAGTCCGTGAAGCCATAAAAACAAGACTCCGTACTGT- GATCTTAGC TCCAACCAGGGTTGTCGCTGCTGAAATGGAGGAAGCCCTTAGAGGGCTTCCAGTGCGTTATATGACAACAGCAG- TCAATGTCA CCCACTCTGGAACAGAAATCGTCGACTTAATGTGCCATGCCACCTTCACTTCACGTCTACTACAGCCAATCAGA- GTCCCCAACTA TAATCTGTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAAGAGGATACATTTCAACAA- GGGTTGAGA TGGGCGAGGCGGCTGCCATCTTCATGACCGCCACGCCACCAGGAACCCGTGACGCATTTCCGGACTCCAACTCA- CCAATTATG GACACCGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTCAGGCTTTGATTGGGTGACGGATCATTCTGGAAAAAC- AGTTTGGT TTGTTCCAAGCGTGAGGAACGGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAACGGGTCATACAGCTC- AGCAGAAA GACTTTTGAGACAGAGTTCCAGAAAACAAAACATCAAGAGTGGGACTTTGTCGTGACAACTGACATTTCAGAGA- TGGGCGCCA ACTTTAAAGCTGACCGTGTCATAGATTCCAGGAGATGCCTAAAGCCGGTCATACTTGATGGCGAGAGAGTCATT- TTGGCTGGA CCCATGCCTGTCACACATGCCAGCGCTGCCCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGGAGA- TGAGTATC TGTATGGAGGTGGGTGCGCAGAGACTGACGAAGACCATGCACACTGGCTTGAAGCAAGAATGCTCCTTGACAAT- ATTTACCTC CAAGATGGCCTCATAGCCTCGCTCTATCGACCTGAGGCCGACAAAGTAGCAGCCATTGAGGGAGAGTTCAAGCT- TAGGACGG AGCAAAGGAAGACCTTTGTGGAACTCATGAAAAGAGGAGATCTTCCTGTTTGGCTGGCCTATCAGGTTGCATCT- GCCGGAATA ACCTACACAGATAGAAGATGGTGCTTTGATGGCACGACCAACAACACCATAATGGAAGACAGTGTGCCGGCAGA- GGTGTGGA CCAGACACGGAGAGAAAAGAGTGCTCAAACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCCTG- AAGTCATT CAAGGAGTTTGCCGCTGGGAAAAGAGGAGCGGCTTTTGGAGTGATGGAAGCCCTGGGAACACTGCCAGGACACA- TGACAGA GAGATTCCAGGAAGCCATTGACAACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGCCTTACAAAGCCG- CGGCGGCC CAATTGCCGGAGACCCTAGAGACCATTATGCTTTTGGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTTTTCGT- CTTGATGAGG AACAAGGGCATAGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGCGCATGGCTCATGTGGCTCTCGGA- AATTGAGC CAGCCAGAATTGCATGTGTCCTCATTGTTGTGTTCCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAA- AGATCTCCCC AGGACAACCAAATGGCAATCATCATCATGGTAGCAGTAGGTCTTCTGGGCTTGATTACCGCCAATGAACTCGGA- TGGTTGGAG AGAACAAAGAGTGACCTAAGCCATCTAATGGGAAGGAGAGAGGAGGGAGCAACCATAGGATTCTCAATGGACAT- TGACCTG CGGCCAGCCTCAGCTTGGGCCATCTATGCTGCCTTGACAACTTTCATTACCCCAGCCGTCCAACATGCAGTGAC- CACTTCATACA ACAACTACTCCTTAATGGCGATGGCCACGCAAGCTGGAGTGTTGTTTGGTATGGGCAAAGGGATGCCATTCTAC- GCATGGGAC TTTGGAGTCCCGCTGCTAATGATAGGTTGCTACTCACAATTAACACCCCTGACCCTAATAGTGGCCATCATTTT- GCTCGTGGCGC ACTACATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCGTGCTGCCCAGAAGAGAACGGCAGCTGGCATC- ATGAAGAA CCCTGTTGTGGATGGAATAGTGGTGACTGACATTGACACAATGACAATTGACCCCCAAGTGGAGAAAAAGATGG- GACAGGTG CTACTCATAGCAGTAGCAGTCTCCAGCGCCATACTGTCGCGGACCGCCTGGGGGTGGGGGGAGGCTGGGGCCCT- GATCACAG CCGCAACTTCCACTTTGTGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCTACAGCCACTTCACTGTGTAAC- ATTTTTAGGG GAAGTTACTTGGCTGGAGCTTCTCTAATCTACATAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGGT- GGAACAGG AGAGACCCTGGGAGAGAAATGGAAGGCCCGCTTGAACCAGATGTCGGCCCTGGAGTTCTACTCCTACAAAAAGT- CAGGCATC ACCGAGGTGTGCAGAGAAGAGGCCCGCCGCGCCCTCAAGGATGGTGTGGCAACGGGAGGCCATGCTGTGTCCCG- AGGAAGT GCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCTGCAGCCCTATGGAAAGGTCATTGATCTTGGATGTGGCAG- AGGGGGC TGGAGTTACTACGCCGCCACCATCCGCAAAGTTCAAGAAGTGAAAGGATACACAAAAGGAGGCCCTGGTCATGA- AGAACCCG TGTTGGTGCAAAGCTATGGGTGGAACATAGTCCGTCTTAAGAGTGGGGTGGACGTCTTTCATATGGCGGCTGAG- CCGTGTGA CACGTTGCTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAAGCACGGACGCTCAGAGTCCTCT- CCATGGTGG GGGATTGGCTTGAAAAAAGACCAGGAGCCTTTTGTATAAAAGTGTTGTGCCCATACACCAGCACTATGATGGAA- ACCCTGGAG CGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGAGTGCCACTCTCCCGCAACTCTACACATGAGATGTACTG- GGTCTCTG GAGCGAAAAGCAACACCATAAAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCGCATGGACGGGCCTAGG- AGGCCAGT GAAATATGAGGAGGATGTGAATCTCGGCTCTGGCACGCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACATGA- AGATCATT GGTAACCGCATTGAAAGGATCCGCAGTGAGCACGCGGAAACGTGGTTCTTTGACGAGAACCACCCATATAGGAC- ATGGGCTT ACCATGGAAGCTATGAGGCCCCCACACAAGGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGTCA- AAACCCTGG GATGTGGTGACTGGAGTCACAGGAATAGCCATGACCGACACCACACCGTATGGTCAGCAAAGAGTTTTCAAGGA- AAAAGTGG ACACTAGGGTGCCAGACCCCCAAGAAGGCACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTTGTGGAAAGAG- CTAGGCAA ACACAAACGGCCACGAGTCTGTACCAAAGAAGAGTTCATCAACAAGGTTCGTAGCAATGCAGCATTAGGGGCAA- TATTTGAA GAGGAAAAAGAGTGGAAGACTGCAGTGGAAGCTGTGAACGATCCAAGGTTCTGGGCTCTAGTGGACAAGGAAAG- AGAGCAC CACCTGAGAGGAGAGTGCCAGAGTTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAACAAGGGGAATTTGG- AAAGGCC AAGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAGATTTCTAGAGTTCGAAGCCCTTGGATTCTTGAA- CGAGGATCA CTGGATGGGGAGAGAGAACTCAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGATATGTCCTAGAAG- AGATGAG TCGCATACCAGGAGGAAGGATGTATGCAGATGACACTGCTGGCTGGGACACCCGCATCAGCAGGTTCGATCTGG- AGAATGAA GCTCTAATCACCAACCAAATGGAGAAAGGGCATAGGGCCTTGGCATTGGCCATAATCAAGTACACATACCAAAA- CAAAGTGGT AAAGGTCCTTAGACCAGCTGAAAAAGGGAAAACAGTTATGGACATTATTTCGAGACAAGACCAAAGGGGGAGCG- GACAAGTT GTCACTTACGCTCTTAACACATTTACCAACCTAGTGGTGCAACTCATTCGGAATATGGAGGCTGAGGAAGTTCT- AGAGATGCAA GACTTGTGGCTGCTGCGGAGGTCAGAGAAAGTGACCAACTGGTTGCAGAGCAACGGATGGGATAGGCTCAAACG- AATGGCA GTCAGTGGAGATGATTGCGTTGTGAAGCCAATTGATGATAGGTTTGCACATGCCCTCAGGTTCTTGAATGATAT- GGGAAAAGT TAGGAAGGACACACAAGAGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAAGTTCCGTTTTGCTCCCACC- ACTTCAACA AGCTCCATCTCAAGGACGGGAGGTCCATTGTGGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCCCGC- GTCTCTCCA GGGGCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGCAAATGTGGCAGCTCCTTTATTT- CCACAGAA GGGACCTCCGACTGATGGCCAATGCCATTTGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGGAGAACTACC- TGGTCAATCC ATGGAAAGGGAGAATGGATGACCACTGAAGACATGCTTGTGGTGTGGAACAGAGTGTGGATTGAGGAGAACGAC- CACATGG AAGACAAGACCCCAGTTACGAAATGGACAGACATTCCCTATTTGGGAAAAAGGGAAGACTTGTGGTGTGGATCT- CTCATAGG GCACAGACCGCGCACCACCTGGGCTGAGAACATTAAAAACACAGTCAACATGGTGCGCAGGATCATAGGTGATG- AAGAAAAG TACATGGACTACCTATCCACCCAAGTTCGCTACTTGGGTGAAGAAGGGTCTACACCTGGAGTGCTGTAAGCACC- AATCTTAATG TTGTCAGGCCTGCTAGTCAGCCACAGCTTGGGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGAAGCTGGGAA- ACCAAGCC TATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTGAGCCCCTCAGAGGACACTGAGTCA- AAAAACCC CATGCGCTTGGAGGCGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAACTGG- AGATCAGC TGTGGATCTCCAGAAGAGGGACTAGTGGTTAGAGGAGACCCCCCGGAAAACGCAAAACAGCATATTGACGCTGG- GAAAGAC CAGAGACTCCATGAGTTTCCACCACGCTGGCCGCCAGGCACAGATCGCCGAATAGCGGCGGCCGGTGTGGGGAA- ATCCATGG GTCTT KU681081.3 Zika virus isolate Zika virus/H. sapiens-tc/THA/2014/SV0127-14, Thailand, complete genome SEQ ID NO: 7 AGTTGTTGATCTGTGTGAATCAGACTGCGACAGTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTT- TTATTTTGG ATTTGGAAACGAGAGTTTCTGGTCATGAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATATGCT- AAAACGCGG AGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGTCATGGGCCCATCA- GGATGGTC TTGGCGATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAGATGGGGTTCAGT- GGGAAAAAA AGAGGCTATGGAAATAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGCTGAGAATAATCAATGCTAGGAAGG- AGAAGAA GAGACGAGGCACAGATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGCGGAGGTCACTA- GACGTGGG AGTGCATACTATATGTACTTGGACAGAAGCGATGCTGGGGAGGCCATATCTTTTCCAACCACACTGGGGATGAA- TAAGTGTTA TATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGG- TAGAACCAG ATGACGTCGATTGTTGGTGCAACACGACGTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAAAAGGTGAA- GCACGGAG ATCCAGAAGAGCTGTGACGCTCCCCTCCCATTCCACTAGGAAGCTGCAAACGCGGTCGCAGACCTGGTTGGAAT- CAAGAGAAT ACACAAAGCACTTGATTAGAGTCGAAAATTGGATATTCAGGAACCCTGGCTTCGCGTTAGCAGCAGCTGCCATC- GCTTGGCTTT TGGGAAGCTCAACGAGCCAAAAAGTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGG- TGCATAGGA

GTCAGTAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTGATGTTGTCTTGGAACATGGAGGTTG- TGTCACCGT AATGGCACAGGACAAACCGACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGTAAGAT- CCTACTGC TATGAGGCATCAATATCGGACATGGCTTCGGACAGCCGCTGCCCAACACAAGGTGAAGCCTACCTTGACAAGCA- ATCAGACAC TCAATATGTCTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGCC- TGGTGACA TGCGCTAAGTTTGCATGCTCCAAGAAAATGACCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAAT- GCTGTCAG TTCATGGCTCCCAGCACAGTGGGATGATCGTTAATGACACAGGACATGAAACTGATGAGAATAGAGCGAAGGTT- GAGATAAC GCCCAATTCACCAAGAGCCGAAGCCACCCTGGGGGGTTTTGGAAGCCTAGGACTTGATTGTGAACCGAGGACAG- GCCTTGAC TTTTCAGATTTGTATTACTTGACTATGAACAACAAGCACTGGTTGGTTCACAAGGAGTGGTTCCACGACATTCC- ATTACCTTGGC ACACTGGGGCAGACACCGGAACTCCACACTGGAACAACAAAGAAGCACTGGTAGAGTTCAAGGACGCACATGCC- AAAAGGC AAACTGTCGTGGTTCTAGGGAGTCAAGAAGGAGCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATG- GATGGTGC AAAGGGAAGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATAAACTTAGATTGAAGGGCGTGTCAT- ACTCCTTGT GTACCGCAGCGTTCACATTCACCAAGATCCCGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGTACAGTAC- GCAGGGAC AGATGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGACATGCAAACTCTGACCCCAGTTGGGAGGTTGATAA- CCGCTAACC CCGTAATCACTGAAGGCACTGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACATT- GTCATAGGA GTCGGGGAGAAGAAGATCACCCACCACTGGCACAGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGT- GAGAGGT GCCAAGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGTTGGAGGCGTTCTTAACTCATTGGG- CAAGGGCA TCCATCAAATTTTTGGAGCAGCTTTCAAATCATTGTTTGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGA- ACGTTGCTGAT GTGGTTGGGTCTGAATACAAAGAATGGATCTATTTCCCTTATGTGCTTGGCCTTAGGGGGAGTGTTGATCTTCT- TATCCACAGC CGTCTCCGCTGATGTGGGGTGCTCGGTGGACTTCTCAAAGAAGGAAACGAGATGCGGTACAGGGGTGTTCGTCT- ATAACGAC GTTGAAGCCTGGAGGGACAGGTACAAGTACCATCCTGACTCCCCTCGTAGATTGGCAGCAGTAGTCAAGCAAGC- CTGGGAAG ATGGTATCTGTGGGATCTCCTCTGTTTCAAGAATGGAAAACATCATGTGGAGATCAGTAGAAGGGGAGCTCAAC- GCAATCCTG GAAGAGAATGGAGTTCAACTGACGGTCGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATT- GCCCGTGC CTGTGAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCGTACTTCGTCAGAGCAGCAAAGACAAATAAC- AGCTTTGT CGTGGATGGTGACACACTGAAGGAATGCCCACTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAGGATCATG- GGTTCGGG GTATTTCACACTAGTGTCTGGCTCAAGGTTAGAGAAGATTATTCACTAGAGTGTGATCCAGCCGTCATTGGAAC- AGCTGTTAAG GGAAAGGAGGCTGTACACAGTGATCTAGGCTACTGGATTGAGAGTGAGAAGAACGACACATGGAGGCTGAGGAG- GGCCCAC CTGATCGAGATGAAAACATGTGAATGGCCAAAGTCCCACACATTGTGGACAGATGGAATAGAAGAGAGTGATCT- GATCATAC CCAAGTCTTTAGCTGGGCCACTCAGCCATCACAACACCAGAGAGGGCTACAGGACCCAAATGAAAGGGCCATGG- CACAGTGA AGAGCTTGAAATTCGGTTTGAGGAATGCCCAGGCACTAAGGTCCACGTGGAGGAAACATGTGGAACAAGAGGAC- CATCTCTG AGATCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGTGCTGCAGGGAGTGCACAATGCCCCCACTGTCGTT- CCGGGCTA AAGATGGCTGTTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAAGTAACTTAGTAAGGTCAATGGTG- ACTGCAG GATCAACTGATCACATGGATCACTTTTCCCTTGGAGTGCTTGTGATTCTGCTCATGGTGCAGGAAGGGCTGAAG- AAGAGAATG ACCACAAAGATCATCATAAGCACATCAATGGCAGTGCTGGTAGCTATGATCCTGGGAGGATTTTCAATGAGTGA- TCTGGCTAA GCTTGCAATTTTGATGGGTGCCACCTTTGCGGAAATGAACACTGGAGGAGATGTAGCTCATCTGGCGCTGGTAG- CGGCATTCA AAGTCAGACCAGCGTTGCTGGTATCTTTCATCTTCAGAGCTAATTGGACACCCCGTGAAAGCATGCTGCTGGCC- TTGGCCTCGT GTCTTTTGCAAACTGCGATCTCCGCCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGG- TTGGCAATAC GAGCGATGGTTGTTCCACGCACTGACAATATCACCTTGGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGC- ACACTGCTTG TGGCGTGGAGAGCAGGCCTTGCTACTTGCGGGGGGTTCATGCTCCTCTCTCTGAAGGGGAAAGGCAGTGTGAAG- AAGAACTT ACCATTTGTCATGGCCCTGGGACTAACCGCTGTGAGGCTGGTCGACCCCATCAACGTGGTGGGACTGCTGTTGC- TCACAAGGA GTGGGAAGCGGAGCTGGCCCCCTAGCGAAGTACTCACAGCTGTTGGCCTGATATGCGCATTGGCTGGAGGGTTC- GCCAAGGC AGATATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAGGAAAGAGTG- TGGACATG TACATTGAAAGAGCAGGTGACATCACATGGGAAAAAGATGCGGAAGTTACTGGAAACAGTCCCCGGCTCGATGT- GGCACTAG ATGAGAGTGGTGATTTCTCCCTGGTGGAGGATGACGGTCCCCCCATGAGAGAGATCATACTCAAAGTGGTCCTG- ATGACCATC TGTGGCATGAACCCAATAGCCATACCCTTTGCAGCTGGAGCGTGGTACGTATACGTGAAAACTGGAAAAAGGAG- TGGTGCTCT ATGGGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCACAGATGGAGTGTACAGAGTAATGACTCGTA- GACTGCTA GGTTCAACACAAGTTGGAGTGGGAGTTATGCAAGAGGGGGTCTTTCACACTATGTGGCATGTCACAAAAGGATC- CGCGCTGA GAAGCGGTGAAGGGAGACTTGATCCATACTGGGGAGATGTCAAGCAGGATCTGGTGTCATACTGTGGTCCATGG- AAGCTAGA TGCCGCCTGGGACGGGCACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGAGAGCGAGGAACATCCAGA- CTCTGCCC GGAATATTTAAGACAAAGGATGGGGACATTGGAGCGGTTGCGCTGGACTATCCAGCAGGAACTTCAGGATCTCC- AATCCTAG ACAAGTGTGGGAGAGTGATAGGACTCTATGGCAATGGGGTCGTGATCAAGAATGGGAGTTATGTCAGTGCCATC- ACCCAAGG GAGGAGGGAGGAAGAGACTCCTGTTGAGTGCTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTAACTGTCTTAG- ACTTGCAT CCTGGAGCTGGGAAAACCAGGAGAGTTCTTCCTGAAATAGTCCGTGAAGCCATAAAAACGAGACTCCGTACTGT- GATCTTAGC TCCAACCAGGGTTGTCGCTGCTGAAATGGAGGAAGCCCTTAGAGGGCTTCCAGTGCGTTATATGACAACAGCAG- TCAATGTCA CCCATTCTGGGACAGAAATCGTTGACTTAATGTGCCATGCCACCTTCACTTCACGTCTACTACAGCCAATCAGA- GTCCCCAACTA TAATCTGTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAAGAGGATACATTTCAACAA- GGGTTGAGA TGGGCGAGGCAGCTGCCATCTTCATGACCGCCACGCCACCAGGAACCCGTGACGCATTCCCGGACTCCAACTCA- CCAATTATG GACACCGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTCAGGCTTTGATTGGGTGACGGATCATTCTGGAAAAAC- AGTTTGGT TTGTCCCAAGCGTGAGGAACGGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAACGGGTCATACAGCTC- AGCAGAAA GACTTTTGAGACAGAGTTCCAGAAAACAAAACATCAAGAGTGGGACTTCGTCGTGACAACTGACATTTCAGAGA- TGGGCGCCA ACTTTAAAGCTGACCGTGTCATAGATTCCAGGAGATGCCTAAAGCCGGTCATACTTGATGGCGAGAGAGTCATT- CTGGCTGGA CCCATGCCTGTCACACATGCCAGCGCTGCCCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGGAGA- TGAGTATC TGTATGGAGGTGGGTGCGCAGAGACTGATGAAGACCATGCACACTGGCTTGAAGCAAGAATGCTCCTTGACAAT- ATTTACCTC CAAGATGGCCTCATAGCCTCGCTCTATCGACCTGAGGCCGACAAAGTAGCAGCCATTGAGGGAGAGTTCAAGCT- TAGGACGG AGCAAAGGAAGACCTTTGTGGAACTCATGAAAAGAGGAGATCTTCCTGTTTGGCTGGCCTATCAGGTTGCATCT- GCCGGAATA ACCTACACAGATAGAAGATGGTGCTTTGATGGCATGACCAACAACACCATAATGGAAGACAGTGTGCCGGCAGA- GGTGTGGA CCAGACACGGAGAGAAAAGAGTGCTCAAACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCCTG- AAGTCATT CAAGGAGTTTGCCGCTGGGAAAAGAGGAGCGGCTTTTGGAGTGATGGAAGCCCTGGGAACACTGCCAGGACACA- TGACGGA GAGATTCCAGGAAGCCATTGACAACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGCCTTACAAAGCCG- CGGCGGCC CAATTGCCGGAGACCCTAGAGACCATTATGCTTTTGGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTTTTCGT- CTTGATGCGG AACAAGGGCATAGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGCGCATGGCTCATGTGGCTCTCGGA- AATTGAGC CAGCCAGAATTGCATGCGTCCTCATTGTTGTGTTCCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAA- AGATCCCCCC AGGACAACCAAATGGCAATCATCATCATGGTAGCAGTAGGTCTTCTGGGCTTGATTACCGCCAATGAACTCGGA- TGGTTGGAG AGAACAAAGAGTGACCTAAGCCATCTAATGGGAAGGAGAGAGGAGGGGGCAACCATAGGATTCTCAATGGACAT- TGACCTG CGGCCAGCCTCGGCCTGGGCCATCTATGCTGCCCTGACAACTTTCATTACCCCAGCCGTCCAACATGCAGTGAC- CACTTCATAC AACAACTACTCCTTAATGGCGATGGCCACGCAAGCTGGAGTGTTGTTTGGTATGGGCAAAGGGATGCCATTCTA- CGCATGGGA CTTTGGAGTCCCGCTGCTAATGATAGGTTGCTACTCACAATTAACACCCCTGACCCTAATAGTGGCTATCATTT- TGCTCGTGGCG CACTACATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCGTGCTGCCCAGAAGAGAACGGCAGCTGGCAT- CATGAAGA ACCCTGTTGTGGATGGAATAGTGGTGACTGACATTGACACAATGACTATTGACCCCCAAGTGGAGAAAAAGATG- GGACAGGT GCTACTCATAGCAGTAGCCGTCTCCAGCGCCATACTGTCGCGGACCGCCTGGGGGTGGGGGGAAGCTGGGGCCC- TGATCACA GCTGCAACTTCCACTTTGTGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCTACAGCCACTTCACTGTGCAA- CATTTTTAGG GGAAGTTACTTGGCTGGAGCTTCTCTAATCTACACAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGG- TGGAACAG GAGAGACCCTGGGAGAGAAATGGAAGGCCCGCTTGAACCAGATGTCGGCCCTGGAGTTCTACTCCTACAAAAAG- TCAGGCAT CACCGAGGTGTGCAGAGAAGAGGCCCGCCGCGCCCTCAAGGACGGTGTGGCAACGGGAGGCCATGCTGTGTCCC- GAGGAAG TGCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCTGCAGCCCTATGGAAAGGTCATTGATCTTGGATGTGGCA- GAGGGGG

CTGGAGTTACTACGCCGCCACCATCCGCAAAGTTCAAGAAGTGAAAGGATACACAAAAGGAGGCCCTGGTCATG- AAGAACCC ATGTTGGTGCAAAGCTATGGGTGGAACATAGTCCGTCTTAAGAGTGGGGTGGACGTCTTTCATATGGCGGCTGA- GCCGTGTG ACACGTTGCTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAAGCACGGACGCTCAGAGTCCTC- TCCATGGTG GGGGATTGGCTTGAAAAAAGACCAGGAGCCTTTTGTGTAAAAGTGTTGTGCCCATACACCAGCACTATGATGGA- AACCCTGGA GCGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGAGTGCCACTCTCCCGCAACTCTACACATGAGATGTACT- GGGTCTCT GGAGCGAAAAGCAACACCATAAAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCGCATGGACGGGCCCAG- GAGGCCA GTGAAATATGAGGAGGATGTGAATCTCGGCTCTGGCACGCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACAT- GAAGATCA TTGGTAACCGCATTGAAAGGATCCGCAGTGAGCACGCGGAAACGTGGTTCTTTGACGAGAACCACCCATATAGG- ACATGGGC TTACCATGGAAGCTATGAGGCCCCTACACAAGGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGT- CAAAACCCT GGGATGTGGTGACTGGAGTCACAGGAATAGCCATGACCGACACCACACCGTATGGTCAGCAAAGAGTTTTCAAG- GAAAAAGT GGACACCAGGGTGCCAGACCCCCAAGAAGGCACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTTGTGGAAAG- AGCTAGGC AAACACAAACGGCCACGAGTCTGTACCAAAGAAGAGTTCATCAACAAGGTTCGTAGCAATGCAGCATTAGGGGC- AATATTTGA AGAGGAAAAAGAGTGGAAGACCGCAGTGGAAGCTGTGAACGATCCAAGGTTCTGGGCTCTAGTGGACAAGGAAA- GAGAGC ACCACCTGAGAGGAGAGTGCCAGAGCTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAACAAGGGGAATTT- GGAAAG GCCAAGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAGATTTCTAGAGTTCGAAGCCCTTGGATTCTT- AAATGAGGA TCACTGGATGGGGAGAGAGAACTCAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGATATGTCCTAG- AAGAGAT GAGTCGCATACCAGGAGGAAGGATGTATGCAGATGACACTGCTGGCTGGGACACCCGCATCAGCAGGTTTGATC- TGGAGAAT GAAGCTTTAATCACCAACCAAATGGAGAAAGGGCACAGGGCCTTAGCATTGGCCATAATCAAGTACACATACCA- AAACAAAGT GGTAAAGGTCCTTAGACCAGCTGAAAAAGGGAAGACAGTTATGGACATTATTTCAAGACAAGACCAAAGGGGGA- GCGGACA AGTTGTCACTTACGCTCTTAACACATTTACCAACCTAGTGGTGCAACTCATTCGGAATATGGAGGCTGAGGAAG- TTCTAGAGAT GCAAGACTTGTGGCTGCTGCGGAGGTCAGAGAAAGTGACCAACTGGTTGCAGAGCAACGGATGGGATAGGCTCA- AACGAAT GGCAGTCAGTGGAGATGATTGCGTTGTGAAGCCAATTGATGATAGGTTTGCACATGCCCTCAGGTTCTTGAATG- ATATGGGAA AAGTTAGGAAGGACACACAAGAGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAAGTTCCGTTTTGTTCC- CACCACTTC AACAAGCTCCATCTCAAGGACGGGAGGTCCATTGTGGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGC- CCGTGTCTC TCCAGGGGCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAGTCATATGCGCAAATGTGGCAGCTCCTTT- ATTTCCACA GAAGGGACCTCCGACTGATGGCCAATGCCATCTGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGGAGAACT- ACCTGGTCA ATCCATGGAAAGGGAGAATGGATGACCACTGAAGACATGCTTGTGGTGTGGAACAGAGTGTGGATTGAGGAGAA- CGACCAC ATGGAAGACAAGACCCCAGTTACGAAATGGACAGACATTCCCTATCTGGGAAAAAGGGAAGACTTGTGGTGTGG- ATCTCTCA TAGGGCACAGACCGCGCACCACCTGGGCTGAGAACATTAAAAACACAGTCAACATGGTGCGCAGGATCATAGGT- GATGAAGA AAAGTACATGGACTACCTATCCACCCAAGTTCGCTACTTGGGTGAAGAAGGGTCTACACCTGGAGTGCTATAAG- CACCAATCTT AGTGTTGTCAGGCCTGCTAGTCAGCCACAGCTTGGGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGAGGCTG- GGAAACCA AGCCCATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTGAGCCCCTCAGAGGACACTGA- GTCAAAAA ACCCCACGCGCTTGGAGGCGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAA- CTGGAGAT CAGCTGTGGATCTCCAGAAGAGGGACTAGTGGTTAGAGGAGACCCCCCGGAAAACGCAAAACAGCATATTGACG- CTGGGAA AGACCAGAGACTCCATGAGTTTCCACCACGCTGGCCGCCAGGCACAGATCGCCGAATAGCGGCGGCCGGTGTGG- GGAAATCC ATGGGTCT KU681082.3 Zika virus isolate Zika virus/H. sapiens-tc/PHL/2012/CPC-0740, Philippines, complete genome SEQ ID NO: 8 AGTTGTTGATCTGTGTGAATCAGACTGCGACAGTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTT- TTATTTTGG ATTTGGAAACGAGAGTTTCTGGTCATGAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATATGCT- AAAACGCGG AGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGCCATGGGCCCATCA- GGATGGTC TTGGCGATACTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAGATGGGGTTCAGT- GGGGAAAAA AGAGGCTATGGAAATAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGCTGAGAATAATCAATGCTAGGAAGG- AGAAGAA GAGACGAGGCGCAGATACTAGCGTCGGAATTGTTGGCCTCCTCCTGACCACAGCCATGGCAGTAGAGGTCACTA- GACGTGGG AGTGCATACTATATGTACTTGGACAGAAGCGATGCTGGGGAGGCCATATCTTTTCCAACCACACTGGGGATGAA- TAAGTGTTA CATACAAATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGAATGCCCTATGTTGGATGAGGGGG- TAGAACCAG ATGACGTCGATTGCTGGTGCAACACGACATCAACTTGGGTTGTGTATGGAACCTGCCACCACAAAAAAGGTGAA- GCACGGAG ATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACTAGGAAGCTGCAAACGCGGTCGCAGACCTGGTTGGAAT- CAAGAGAAT ACACAAAGCACCTGATTAGAGTTGAAAATTGGATATTCAGGAACCCTGGCTTCGCGTTAGCAGCAGCTGTCATC- GCTTGGCTTT TGGGAAGTTCAACGAGCCAAAAAGTCATATATCTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGG- TGCATAGGA GTCAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTGATGTTGTCTTGGAACATGGAGGTTG- TGTTACCGT AATGGCACAGGACAAACCGACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGTAAGAT- CCTACTGC TATGAGGCATCAATATCGGATATGGCTTCGGACAGCCGCTGCCCAACACAAGGTGAGGCCTACCTTGACAAGCA- GTCAGACAC TCAATATGTCTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGCC- TGGTGACA TGCGCTAAGTTTGCATGCTCCAAGAAAATGACCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAAT- GCTGTCAG TTCATGGCTCCCAGCACAGTGGGATGATCGTTAATGACACAGGACATGAAACTGATGAGAATAGAGCGAAGGTT- GAGATAAC GCCCAATTCACCAAGAGCCGAAGCCACCCTGGGGGGTTTTGGGAGCCTAGGACTTGATTGTGAACCGAGGACAG- GCCTTGAC TTTTCAGATTTGTATTACCTGACTATGAATAACAAGCACTGGTTGGTTCACAAGGAGTGGTTCCACGACATTCC- ATTACCTTGGC ATGCTGGGGCAGACACTGGAACTCCACATTGGAACAACAAAGAAGCACTGGTAGAGTTCAAGGACGCACATGCA- AAAAGGCA AACTGTCGTGGTTCTAGGGAGTCAAGAAGGAGCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGG- ATGGAGCC AAGGGAAGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATAAACTTAGATTGAAGGGCGTGTCATA- CTCCTTGTG CACTGCAGCGTTCACATTCACCAAGATCCCGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGTACAGTACG- CAGGGACA GATGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGATATGCAAACTCTGACCCCAGTTGGGAGGTTGATAAC- CGCTAACCC TGTAATCACTGAAAGCACCGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACATTG- TCATAGGAGT CGGGGAGAAGAAGATCACCCATCACTGGCACAGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGA- GAGGTGC CAAGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGTTGGGGGTGCTCTCAACTCATTGGGCA- AGGGCATC CATCAAATTTTTGGAGCAGCTTTCAAATCATTGTTCGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGAAC- GTTGCTGGTGT GGTTGGGTCTGAATACAAAGAATGGATCTATTTCCCTTACGTGCTTGGCCTTAGGGGGAGTGTTGATCTTCTTA- TCCACAGCCG TTTCTGCTGATGTGGGGTGCTCGGTGGACTTCTCAAAGAAGGAAACGAGATGCGGTACAGGGGTGTTCGTCTAT- AACGACGTT GAAGCCTGGAGGGACAGGTACAAGTACCATCCTGACTCCCCTCGTAGATTGGCAGCAGCAGTCAAGCAAGCCTG- GGAAGATG GGATCTGTGGGATCTCCTCTGTCTCAAGAATGGAAAACATCATGTGGAGATCAGTAGAAGGGGAGCTCAACGCA- ATCCTGGA AGAGAATGGAGTTCAACTGACGGTCGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATTGC- CCGTGCCT GTGAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCGTACTTCGTCAGAGCAGCAAAGACAAATAACAG- CTTTGTCG TGGATGGTGACACACTGAAGGAATGCCCACTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAGGATCATGGG- TTTGGGGT ATTTCACACTAGTGTCTGGCTCAAGGTTAGAGAAGATTATTCATTAGAGTGTGATCCAGCCGTCATTGGAACAG- CTGCTAAGG GAAAGGAGGCTGTGCACAGCGATCTAGGCTACTGGATTGAGAGTGAGAAGAACGACACATGGAGGCTGAAGAGG- GCCCACC TGATCGAGATGAAAACATGTGAATGGCCAAAGTCCCACACATTGTGGACAGATGGAGTAGAAGAAAGTGATCTG- ATCATACC CAAGTCTTTAGCTGGGCCACTCAGCCATCACAACACCAGAGAGGGCTACAGGACTCAAATGAAAGGGCCATGGC- ACAGTGAA GAGCTTGAAATTCGGTTTGAGGAATGCCCAGGCACTAAGGTCCACGTGGAGGAAACATGTGGGACAAGAGGACC- ATCCCTGA GATCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGTGCTGCAGGGAATGCACAATGCCCCCACTGTCGTTC- CGAGCTAA AGATGGCTGTTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAAGTAACTTAGTAAGGTCAATGGTGA- CTGCAGG ATCAACTGATCACATGGATCACTTCTCTCTTGGAGTGCTTGTGATTTTGCTCATGGTGCAGGAAGGGCTGAAGA- AGAGAATGA CCACAAAGATCATCATAAGCACATCAATGGCAGTGCTGGTAGCCATGATCCTGGGAGGATTTTCAATGAGTGAC- CTGGCTAAG CTTGCAATTTTGATGGGTGCCACCTTCGCGGAAATGAACACTGGAGGAGATGTAGCTCATTTGGCGCTGATAGC- GGCATTCAA AGTCAGACCTGCGTTGCTGGTATCTTTCATCTTCAGAGCTAATTGGACACCCCGTGAGAGCATGCTGCTGGCCT- TGGCCTCGTG

TCTTCTGCAAACTGCGATCTCCGCCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGGT- TGGCAATACG AGCGATGGTTGTTCCACGCACTGACAACATCACCTTGGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCA- CACTGCTTGT GGCGTGGAGAGCAGGCCTTGCTACTTGCGGGGGGTTCATGCTCCTCTCTCTGAAGGGGAAAGGCAGTGTGAAGA- AGAACCTA CCATTTGTCATGGCCTTGGGACTAACTGCTGTGAGGCTGGTCGACCCCATCAACGTGGTGGGACTGCTGTTGCT- CACAAGGAG TGGGAAGCGGAGCTGGCCCCCTAGTGAAGTACTCACAGCTGTTGGCCTGATATGCGCATTGGCTGGAGGGTTCG- CCAAGGCG GATATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAGGAAAGAGTGT- GGACATGT ACATTGAAAGAGCAGGTGACATCACATGGGAAAAAGATGCGGAAATCACTGGAAACAGTCCCCGGCTCGATGTG- GCACTAGA TGAGAGTGGTGATTTCTCCCTAGTGGAGGATGATGGTCCACCCATGAGAGAGATCATACTCAAAGTGGTCCTGA- TGACCATCT GCGGCATGAACCCAATAGCCATACCCTTTGCAGCTGGAGCGTGGTACGTGTATGTGAAGACTGGAAAAAGGAGT- GGTGCTCT ATGGGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCACAGATGGAGTGTACAGAGTAATGACTCGTA- GACTGCTT GGTTCAACACAAGTTGGAGTGGGAGTCATGCAAGAGGGGGTCTTCCACACTATGTGGCACGTCACAAAAGGATC- CGCGCTGA GAAGCGGTGAAGGGAGACTTGATCCATACTGGGGAGATGTCAAGCAGGATCTGGTGTCATACTGTGGTCCGTGG- AAGCTAGA CGCCGCCTGGGACGGGCACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGAGAGCGAGGAACATCCAGA- CTCTGCC CGGAACATTTAAGACAAAGGATGGGGACATTGGAGCAGTTGCGCTGGACTACCCAGCAGGAACTTCAGGATCTC- CAATCCTA GACAAGTGTGGGAGAGTGATAGGACTCTATGGTAATGGGGTCGTGATAAAAAATGGGAGTTATGTTAGTGCCAT- CACCCAAG GGAGGAGGGAGGAAGAGACTCCTGTTGAGTGCTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTAACTGTCTTA- GACCTGCA TCCTGGAGCCGGGAAAACCAGGAGAGTTCTTCCTGAAATAGTCCGTGAAGCCATAAAAACAAGACTCCGTACTG- TGATCTTAG CTCCAACCAGGGTCGTCGCTGCTGAAATGGAGGAAGCCCTTAGAGGGCTTCCAGTTCGTTATATGACAACAGCA- GTCAATGTC ACCCATTCTGGGACAGAAATCGTTGACTTAATGTGCCATGCTACCTTCACTTCACGCCTACTACAACCAATCAG- AGTCCCCAACT ATAATTTGTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAAGAGGATACATTTCAACA- AGGGTTGAG ATGGGCGAGGCGGCTGCCATCTTCATGACCGCCACGCCACCAGGAACCCGTGACGCATTCCCGGACTCCAACTC- ACCAATTAT GGACACCGAGGTGGAAGTCCCAGAGAGAGCCTGGAGCACAGGCTTTGATTGGGTGACGGATCATTCTGGGAAAA- CAGTCTG GTTTGTTCCAAGCGTGAGGAACGGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAACGGGTCATACAGC- TCAGCAGA AAGACTTTTGAGACAGAGTTCCAGAAAACGAAAAATCAAGAGTGGGACTTCGTCGTGACAACCGACATTTCAGA- GATGGGCG CCAACTTTAAAGCTGACCGTGTCATAGATTCCAGGAGATGCTTAAAGCCGGTCATACTTGATGGCGAGAGAGTC- ATTTTGGCT GGACCCATGCCTGTCACACATGCCAGCGCTGCTCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGG- AGATGAGT ATCTGTATGGAGGTGGGTGCGCAGAGACTGATGAAGATCACGCACACTGGCTTGAAGCAAGAATGCTTCTTGAC- AACATTTAC CTCCAAGATGGCCTCATAGCTTCGCTCTATCGACCTGAGGCCGACAAAGTAGCAGCTATTGAGGGAGAGTTCAA- GCTTAGGAC GGAGCAAAGGAAGACCTTTGTGGAACTCATGAAAAGAGGAGATCTTCCGGTTTGGTTGGCCTATCAGGTTGCAT- CTGCCGGA ATAACCTACACAGATAGAAGATGGTGCTTTGATGGCATGACCAACAACACCATAATGGAAGACAGTGTGCCGGC- AGAGGTGT GGACCAGATACGGAGAGAAAAGAGTGCTCAAACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCC- CTGAAGT CATTCAAAGAGTTTGCCGCTGGGAAAAGAGGAGCGGCCTTTGGAGTGATAGAAGCCCTGGGAACACTGCCAGGA- CACATGAC AGAGAGATTCCAGGAAGCCATTGACAACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGCCTTACAAAG- CCGCGGCG GCCCAATTGCCGGAGACCCTAGAGACCATTATGCTTTTGGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTTTT- CGTCTTGATG CGGAACAAGGGCATGGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGCGCATGGCTTATGTGGCTCTC- GGAAATTG AGCCAGCCAGAATTGCATGTGTCCTCATTGTCGTGTTCCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAG- CAAAGATCTC CTCAGGACAACCAAATGGCAATCATCATCATGGTAGCAGTGGGTCTTCTGGGCTTGATTACCGCCAATGAACTC- GGATGGTTG GAGAGAACAAAAAGTGACCTAAGCCATCTAATGGGAAGGAGAGAGGAGGGGGCAACCACAGGATTCTCAATGGA- CATTGAC CTGCGGCCAGCCTCAGCTTGGGCTATCTATGCTGCTCTGACAACTTTCATCACCCCAGCCGTCCAACATGCGGT- GACCACTTCAT ACAACAACTACTCCTTAATGGCGATGGCCACGCAAGCTGGGGTGTTGTTTGGTATGGGCAAAGGGATGCCATTC- TACGCATGG GACTTTGGAGTCCCGCTGCTAATGATGGGTTGCTACTCACAATTAACACCTCTGACCCTAATAGTGGCCATCAT- TTTGCTCGTG GCGCACTACATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCGGGCTGCCCAGAAGAGAACGGCAGCTGG- CATCATGA AGAACCCTGTTGTGGATGGAATAGTGGTGACTGACATTGACACAATGACAATTGACCCCCAAGTGGAAAAAAAG- ATGGGGCA GGTGCTACTCATAGCAGTAGCCGTCTCCAGCGCCATACTGTCGCGGACCGCCTGGGGGTGGGGGGAGGCTGGGG- CCCTGATC ACAGCTGCAACTTCCACCTTGTGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCCACAGCCACTTCACTGTG- TAACATTTTTA GGGGAAGTTACTTGGCTGGAGCTTCTCTAATCTACACAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGG- GGTGGAAC GGGAGAGACCCTGGGAGAGAAATGGAAGGCCCGCCTGAACCAGATGTCGGCCCTGGAGTTCTACTCCTACAAAA- AGTCAGGC ATCACCGAGGTGTGCAGAGAAGAGGCCCGCCGTGCCCTCAAGGACGGTGTGGCAACAGGAGGCCATGCTGTGTC- CCGAGGA AGTGCAAAGCTTAGATGGCTGGTGGAGAGAGGATACCTGCAGCCCTATGGAAAGGTCATTGATCTTGGATGTGG- CAGAGGG GGCTGGAGTTACTATGCCGCCACCATCCGCAAAGTTCAGGAAGTGAAAGGATACACAAAAGGAGGCCCTGGTCA- TGAAGAAC CCATGTTGGTGCAAAGCTATGGGTGGAACATAGTCCGTCTTAAGAGTGGGGTGGACGTCTTTCACATGGCGGCT- GAGCCGTG TGACACTTTGCTGTGTGATATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAAGCACGGACGCTCAGAGTCC- TCTCCATGG TGGGGGATTGGCTTGAAAAAAGACCAGGAGCCTTTTGTATAAAAGTGTTGTGCCCATACACCAGCACTATGATG- GAAACCCTG GAGCGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGGGTGCCACTCTCCCGCAACTCTACACATGAGATGTA- CTGGGTCT CTGGAGCGAAAAGCAACACCATAAAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCGCATGGACGGGCCC- AGGAGGCC AGTGAAATATGAGGAGGATGTGAATCTCGGCTCTGGCACGCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACA- TGAAGATC ATTGGTAACCGCATTGAGAGGATCCGCAGTGAGCACGCGGAAACGTGGTTCTTTGACGAGAACCACCCATATAG- GACATGGG CTTACCATGGAAGCTATGAGGCCCCTACACAAGGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTG- TCAAAACCCT GGGATGTGGTGACTGGAGTCACAGGAATAGCCATGACTGACACCACACCGTATGGTCAGCAAAGAGTTTTCAAG- GAAAAAGT GGACACTAGGGTGCCAGACCCCCAAGAAGGCACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTTATGGAAGG- AGCTAGGC AAACACAAACGGCCACGAGTCTGTACCAAAGAAGAGTTCATCAACAAGGTTCGTAGCAATGCAGCATTAGGGGC- AATATTTGA AGAGGAAAAAGAGTGGAAGACTGCAGTGGAAGCTGTGAATGATCCAAGGTTCTGGGCTCTAGTGGACAAGGAAA- GAGAGCA TCACCTGAGAGGAGAGTGTCAGAGCTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAACAAGGGGAATTTG- GAAAGGC CAAGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAGATTCCTAGAGTTCGAAGCCCTTGGATTCTTGA- ATGAGGATC ATTGGATGGGGAGAGAGAATTCAGGAGGTGGTGTTGAAGGACTGGGATTACAAAGACTCGGATATGTCCTAGAA- GAGATGA GTCGCATACCAGGAGGAAGGATGTATGCAGATGATACTGCTGGCTGGGACACCCGCATCAGCAGGTTTGATCTG- GAGAATGA AGCTCTAATCACCAACCAAATGGAGAAAGGGCACAGGGCCTTGGCATTGGCCATAATCAAGTACACATACCAAA- ACAAAGTG GTAAAGGTCCTTAGACCAGCTGAAAAAGGGAAGACAGTTATGGACATTATTTCAAGACAAGACCAAAGGGGGAG- CGGACAA GTTGTCACTTACGCTCTTAATACATTCACCAACCTGGTGGTGCAGCTCATTCGGAATATGGAGGCTGAGGAAGT- TCTAGAGATG CAAGACTTGTGGCTGCTGCGGAGGCCAGAGAAAGTGACCAACTGGTTGCAAAGCAACGGATGGGATAGGCTCAA- AAGAATG GCAGTCAGTGGAGATGATTGCGTTGTGAAACCAATTGATGATAGGTTTGCACATGCCCTCAGGTTCTTGAATGA- TATGGGAAA AGTTAGGAAGGACACACAAGAGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAAGTTCCGTTTTGCTCCC- ACCACTTCA ACAAACTCCATCTTAAGGACGGGAGGTCCATTGTGGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGAGCC- CGCGTATCA CCAGGGGCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGCAAATGTGGCAGCTCCTTTA- TTTCCACAG AAGGGACCTCCGACTGATGGCCAATGCCATTTGTTCATCTGTGCCAGTTGATTGGGTTCCAACTGGGAGAACTA- CCTGGTCAAT CCATGGAAAGGGAGAATGGATGACCACTGAAGACATGCTTGTGGTATGGAACAGAGTGTGGATTGAGGAAAACG- ACCACAT GGAAGACAAGACCCCAGTTACAAAATGGACAGACATTCCCTATTTGGGAAAAAGAGAAGACTTGTGGTGTGGAT- CTCTCATAG GGCACAGACCGCGTACTACCTGGGCTGAGAACATCAAAAATACAGTCAACATGATGCGCAGGATCATAGGTGAT- GAAGAAAA GTACATGGACTACCTATCCACCCAGGTTCGCTACTTGGGTGAAGAAGGGTCCACACCTGGAGTGCTGTAAGCAC- CAATCTTAG TGTTGTCAGGCCTGCTAGTCAGCCACAGCTTGGGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGAAGCTGGG- AAACCAAG CCTATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTGAGCCCCTCAGAGGACACTGAGT- CAAAAAAC CCCACGCGCTTGGAGGCGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAACT- GGAGATCA GCTGTGGATCTCCAGAAGAGGGACTAGTGGTTAGAGGAGACCCCCCGGAAAACGCAAAACAGCATATTGACGCT- GGGAAAG ACCAGAGACTCCATGAGTTTCCACCACGCTGGCCGCCAGGCACAGATCGCCGAATAGCGGCGGCCGGTGTGGGG-

AAATCCAT GGGTCT KU707826.1 Zika virus isolate SSABR1, Brazil, complete genome SEQ ID NO: 9 GACAGTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTTTATTTGGATTTGGAAACGAGAGTTTCT- GGTCATGAA AAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATATGCTAAAACGCGGAGTAGCCCGTGTGAGCCCCT- TTGGGGGC TTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGTCATGGGCCCATCAGGATGGTCTTGGCGATTCTAGCCTTTTT- GAGATTCAC GGCAATCAAGCCATCACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAATAATAA- AGAAGTTC AAGAAAGATCTGGCTGCCATGCTGAGAATAATCAATGCTAGGAAGGAGAAGAAGAGACGAGGCGCAGATACTAG- TGTCGGA ATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGCGGAGGTCACTAGACGTGGGAGTGCATACTATATGTACTT- GGACAGAAA CGATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAAGTGTTATATACAGATCATGGATCTTG- GACACATGT GTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGC- AACACGAC GTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAAAAGGTGAAGCACGGAGATCTAGAAGAGCTGTGACGC- TCCCCTCCC ATTCCACTAGGAAGCTGCAAACGCGGTCGCAAACCTGGTTGGAATCAAGAGAATACACAAAGCACTTGATTAGA- GTCGAAAAT TGGATATTCAGGAACCCTGGCTTCGCGTTAGCAGCAGCTGCCATCGCTTGGCTTTTGGGAAGCTCAACGAGCCA- AAAAGTCAT ATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTCAGCAATAGGGACTTTG- TGGAAGGTA TGTCAGGTGGGACCTGGGTTGATGTTGTCTTGGAACATGGAGGTTGTGTCACCGTAATGGCACAGGACAAACCG- ACTGTCGA CATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGTAAGATCCTACTGCTATGAGGCATCAATATCAG- ACATGGCTT CGGACAGCCGCTGCCCAACACAAGGTGAAGCCTACCTTGACAAGCAATCAGACACTCAATATGTCTGCAAAAGA- ACGTTAGTG GACAGAGGCTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGCCTGGTGACATGCGCTAAGTTTGCATGCTC- CAAGAAAA TGACCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAATGCTGTCAGTTCATGGCTCCCAGCACAGT- GGGATGAT TGTTAATGACACAGGACATGAAACTGATGAGAATAGAGCGAAAGTTGAGATAACGCCCAATTCACCAAGAGCCG- AAGCCACC CTGGGGGGTTTTGGAAGCCTAGGACTTGATTGTGAACCGAGGACAGGCCTTGACTTTTCAGATTTGTATTACTT- GACTATGAAT AACAAGCACTGGTTGGTTCACAAGGAGTGGTTCCACGACATTCCATTACCTTGGCACGCTGGGGCAGACACCGG- AACTCCACA CTGGAACAACAAAGAAGCACTGGTAGAGTTCAAGGACGCACATGCCAAAAGGCAAACTGTCGTGGTTCTAGGGA- GTCAAGAA GGAGCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGGATGGTGCAAAGGGAAGGCTGTCCTCTGG- CCACTTGA AATGTCGCCTGAAAATGGATAAACTTAGATTGAAGGGCGTGTCATACTCCTTGTGTACTGCAGCGTTCACATTC- ACCAAGATCC CGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGTACAGTACGCAGGGACAGATGGACCTTGCAAGGTTCCA- GCTCAGAT GGCGGTGGACATGCAAACTCTGACCCCAGTTGGGAGGTTGATAACCGCTAACCCCGTAATCACTGAAAGCACTG- AGAACTCTA AGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACATTGTCATAGGAGTCGGGGAGAAGAAGATCACC- CACCACTGG CACAGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGAGAGGTGCCAAGAGAATGGCAGTCTTGGG- AGACACA GCCTGGGACTTTGGATCAGTTGGAGGCGCTCTCAACTCATTGGGCAAGGGCATCCATCAAATTTTTGGAGCAGC- TTTCAAATCA TTGTTTGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGAACGTTGCTGATGTGGTTGGGTCTGAACACAAA- GAATGGATCT ATTTCCCTTATGTGCTTGGCCTTAGGGGGAGTGTTGATCTTCTTATCCACAGCCGTCTCTGCTGATGTGGGGTG- CTCGGTGGAC TTCTCAAAGAAGGAGACGAGATGCGGTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTA- CAAGTACC ATCCTGACTCCCCCCGTAGATTGGCAGCAGCAGTCAAGCAAGCCTGGGAAGATGGTATCTGCGGGATCTCCTCT- GTTTCAAGA ATGGAAAACATCATGTGGAGATCAGTAGAAGGGGAGCTCAACGCAATCCTGGAAGAGAATGGAGTTCAACTGAC- GGTCGTTG TGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATTGCCCGTGCCTGTGAACGAGCTGCCCCACGGC- TGGAAGGC TTGGGGGAAATCGTACTTCGTCAGAGCAGCAAAGACAAATAACAGCTTTGTCGTGGATGGTGACACACTGAAGG- AATGCCCA CTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAGGATCATGGGTTCGGGGTATTTCACACTAGTGTCTGGCT- CAAGGTTAG AGAAGATTATTCATTAGAGTGTGATCCAGCCGTTATTGGAACAGCTGTTAAGGGAAAGGAGGCTGTACACAGTG- ATCTAGGCT ACTGGATTGAGAGTGAGAAGAATGACACATGGAGGCTGAAGAGGGCCCATCTGATCGAGATGAAAACATGTGAA- TGGCCAA AGTCCCACACATTGTGGACAGATGGAATAGAAGAGAGTGATCTGATCATACCCAAGTCTTTAGCTGGGCCACTC- AGCCATCAC AATACCAGAGAGGGCTACAGGACCCAAATGAAAGGGCCATGGCACAGTGAAGAGCTTGAAATTCGGTTTGAGGA- ATGCCCA GGCACTAAGGTCCACGTGGAGGAAACATGTGGAACAAGAGGACCATCTCTGAGATCAACCACTGCAAGCGGAAG- GGTGATC GAGGAATGGTGCTGCAGGGAGTGCACAATGCCCCCACTGTCGTTCCGGGCTAAAGATGGCTGTTGGTATGGAAT- GGAGATAA GGCCCAGGAAAGAACCAGAAAGCAACTTAGTAAGGTCAATGGTGACTGCAGGATCAACTGATCACATGGACCAC- TTCTCCCTT GGAGTGCTTGTGATTCTGCTCATGGTGCAGGAAGGGCTGAAGAAGAGAATGACCACAAAGATCATCATAAGCAC- ATCAATGG CAGTGCTGGTAGCTATGATCCTGGGAGGATTTTCAATGAGTGACCTGGCTAAGCTTGCAATTTTGATGGGTGCC- ACCTTCGCG GAAATGAACACTGGAGGAGATGTAGCTCATCTGGCGCTGATAGCGGCATTCAAAGTCAGACCAGCGTTGCTGGT- ATCTTTCAT CTTCAGAGCTAATTGGACACCCCGTGAAAGCATGCTGCTGGCCTTGGCCTCGTGTCTTTTGCAAACTGCGATCT- CCGCCTTGGA AGGCGACCTGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGGTTGGCAATACGAGCGATGGTTGTTCCACGCA- CTGATAACAT CACCTTGGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCACACTGCTTGTGGCGTGGAGAGCAGGCCTTG- CTACTTGCG GGGGGTTTATGCTCCTCTCTCTGAAGGGAAAAGGCAGTGTGAAGAAGAACTTACCATTTGTCATGGCCCTGGGA- CTAACCGCT GTGAGGCTGGTCGACCCCATCAACGTGGTGGGACTGCTGTTGCTCACAAGGAGTGGGAAGCGGAGCTGGCCCCC- TAGCGAA GTACTCACAGCTGTTGGCCTGATATGCGCATTGGCTGGAGGGTTCGCCAAGGCAGATATAGAGATGGCTGGGCC- CATGGCCG CGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAGGAAAGAGTGTGGACATGTACATTGAAAGAGCAGGTGAC- ATCACATG GGAAAAAGATGCGGAAGTCACTGGAAACAGTCCCCGGCTCGATGTGGCGCTAGATGAGAGTGGTGATTTCTCCC- TGGTGGAG GATGACGGTCCCCCCATGAGAGAGATCATACTCAAGGTGGTCCTGATGACCATCTGTGGCATGAACCCAATAGC- CATACCCTTT GCAGCTGGAGCGTGGTACGTATACGTGAAGACTGGAAAAAGGAGTGGTGCTCTATGGGATGTGCCTGCTCCCAA- GGAAGTAA AAAAGGGGGAGACCACAGATGGAGTGTACAGAGTAATGACTCGTAGACTGCTAGGTTCAACACAAGTTGGAGTG- GGAGTTA TGCAAGAGGGGGTCTTTCACACTATGTGGCACGTCACAAAAGGATCCGCGCTGAGAAGCGGTGAAGGGAGACTT- GATCCATA CTGGGGAGATGTCAAGCAGGATCTGGTGTCATACTGTGGTCCATGGAAGCTAGATGCCGCCTGGGACGGGCACA- GCGAGGT GCAGCTCTTGGCCGTGCCCCCCGGAGAGAGAGCGAGGAACATCCAGACTCTGCCCGGAATATTTAAGACAAAGG- ATGGGGAC ATTGGAGCGGTTGCGCTGGATTACCCAGCAGGAACTTCAGGATCTCCAATCCTAGACAAGTGTGGGAGAGTGAT- AGGACTTTA TGGCAATGGGGTCGTGATCAAAAATGGGAGTTATGTTAGTGCCATCACCCAAGGGAGGAGGGAGGAAGAGACTC- CTGTTGA GTGCTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTAACTGTCTTAGACTTGCATCCTGGAGCTGGGAAAACCA- GGAGAGTTC TTCCTGAAATAGTCCGTGAAGCCATAAAAACAAGACTCCGTACTGTGATCTTAGCTCCAACCAGGGTTGTCGCT- GCTGAAATGG AGGAGGCCCTTAGAGGGCTTCCAGTGCGTTATATGACAACAGCAGTCAATGTCACCCACTCTGGAACAGAAATC- GTCGACTTA ATGTGCCATGCCACCTTCACTTCACGTCTACTACAGCCAATCAGAGTCCCCAACTATAATCTGTATATTATGGA- TGAGGCCCACT TCACAGATCCCTCAAGTATAGCAGCAAGAGGATACATTTCAACAAGGGTTGAGATGGGCGAGGCGGCTGCCATC- TTCATGACC GCCACGCCACCAGGAACCCGTGACGCATTTCCGGACTCCAACTCACCAATTATGGACACCGAAGTGGAAGTCCC- AGAGAGAGC CTGGAGCTCAGGCTTTGATTGGGTGACGGATCATTCTGGAAAAACAGTTTGGTTTGTTCCAAGCGTGAGGAACG- GCAATGAG ATCGCAGCTTGTCTGACAAAGGCTGGAAAACGGGTCATACAGCTCAGCAGAAAGACTTTTGAGACAGAGTTCCA- GAAAACAA AACATCAAGAGTGGGACTTTGTCGTGACAACTGACATTTCAGAGATGGGCGCCAACTTTAAAGCTGACCGTGTC- ATAGATTCC AGGAGATGCCTAAAGCCGGTCATACTTGATGGCGAGAGAGTCATTCTGGCTGGACCCATGCCTGTCACACATGC- CAGCGCTGC CCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGGAGATGAGTATCTGTATGGAGGTGGGTGCGCAG- AGACTGA CGAAGACCATGCACACTGGCTTGAAGCAAGAATGCTCCTTGACAATATTTACCTCCAAGATGGCCTCATAGCCT- CGCTCTATCG ACCTGAGGCCGACAAAGTAGCAGCCATTGAGGGAGAGTTCAAGCTTAGGACGGAGCAAAGGAAGACCTTTGTGG- AACTCATG AAAAGAGGAGATCTTCCTGTTTGGCTGGCCTATCAGGTTGCATCTGCCGGAATAACCTACACAGATAGAAGATG- GTGCTTTGA TGGCACGACCAACAACACCATAATGGAAGACAGTGTGCCGGCAGAGGTGTGGACCAGACACGGAGAGAAAAGAG- TGCTCAA ACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCCTGAAGTCATTCAAGGAGTTTGCCGCTGGGA- AAAGAGGA GCGGCTTTTGGAGTGATGGAAGCCCTGGGAACACTGCCAGGACACATGACAGAGAGATTCCAGGAAGCCATTGA- CAACCTCG CTGTGCTCATGCGGGCAGAGACTGGAAGCAGGCCTTACAAAGCCGCGGCGGCCCAATTGCCGGAGACCCTAGAG- ACCATTAT GCTTTTGGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTCTTCGTCTTGATGAGGAACAAGGGCATAGGGAAGA- TGGGCTTT GGAATGGTGACTCTTGGGGCCAGCGCATGGCTCATGTGGCTCTCGGAAATTGAGCCAGCCAGAATTGCATGTGT- CCTCATTGT

TGTGTTTCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAAAGATCTCCCCAGGACAACCAAATGGCAA- TCATCATCAT GGTAGCAGTAGGTCTTCTGGGCTTGATTACCGCCAATGAACTCGGATGGTTGGAGAGAACAAAGAGTGACCTAA- GCCATCTA ATGGGAAGGAGAGAGGAGGGGGCAACCATAGGATTCTCAATGGACATTGACCTGCGGCCAGCCTCAGCTTGGGC- CATCTATG CTGCCTTGACAACTTTCATTACCCCAGCCGTCCAACATGCAGTGACCACTTCATACAACAACTACTCCTTAATG- GCGATGGCCAC GCAAGCTGGAGTGTTGTTTGGTATGGGCAAAGGGATGCCATTCTACGCATGGGACTTTGGAGTCCCGCTGCTAA- TGATAGGTT GCTACTCACAATTAACACCCCTGACCCTAATAGTGGCCATCATTTTGCTCGTGGCGCACTACATGTACTTGATC- CCAGGGCTGCA GGCAGCAGCTGCGCGTGCTGCCCAGAAGAGAACGGCAGCTGGCATCATGAAGAACCCTGTTGTGGATGGAATAG- TGGTGACT GACATTGACACAATGACAATTGACCCCCAAGTGGAGAAAAAGATGGGACAGGTGCTACTCATAGCAGTAGCCGT- CTCCAGCG CCATACTGTCGCGGACCGCCTGGGGGTGGGGGGAGGCTGGGGCCCTGATCACAGCCGCAACTTCCACTTTGTGG- GAAGGCTC TCCGAACAAGTACTGGAACTCCTCTACAGCCACTTCACTGTGTAACATTTTTAGGGGAAGTTACTTGGCTGGAG- CTTCTCTAATC TACACAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGGTGGAACAGGAGAGACCCTGGGAGAGAAATG- GAAGGC CCGCTTGAACCAGATGTCGGCCCTGGAGTTCTACTCCTACAAAAAGTCAGGCATCACCGAGGTGTGCAGAGAAG- AGGCCCGC CGCGCCCTCAAGGACGGTGTGGCAACGGGAGGCCATGCTGTGTCCCGAGGAAGTGCAAAGCTGAGATGGTTGGT- GGAGCGG GGATACCTGCAGCCCTATGGAAAGGTCATTGATCTTGGATGTGGCAGAGGGGGCTGGAGTTACTACGCCGCCAC- CATCCGCA AAGTTCAAGAAGTGAAAGGATACACAAAAGGAGGCCCTGGTCATGAAGAACCCGTGTTGGTGCAAAGCTATGGG- TGGAACAT AGTCCGTCTTAAGAGTGGGGTGGACGTCTTTCATATGGCGGCTGAGCCGTGTGACACGTTGCTGTGTGACATAG- GTGAGTCAT CATCTAGTCCTGAAGTGGAAGAAGCACGGACGCTCAGAGTCCTCTCCATGGTGGGGGATTGGCTTGAAAAAAGA- CCAGGAGC CTTTTGTATAAAGGTGTTGTGCCCATACACCAGCACTATGATGGAAACCCTGGAGCGACTGCAGCGTAGGTATG- GGGGAGGA CTGGTCAGAGTGCCACTCTCCCGCAACTCTACACATGAGATGTATTGGGTCTCTGGAGCGAAAAGCAACACCAT- AAAAAGTGT GTCCACCACGAGCCAGCTCCTCTTGGGGCGCATGGACGGGCCTAGGAGGCCAGTGAAATATGAGGAGGATGTGA- ATCTCGGC TCTGGCACGCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACATGAAGATCATTGGTAACCGCATTGAAAGGAT- CCGCAGTG AGCACGCGGAAACGTGGTTCTTTGACGAGAACCACCCATATAGGACATGGGCTTACCATGGAAGCTATGAGGCC- CCCACACAA GGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGTCAAAACCCTGGGATGTGGTGACTGGAGTCAC- AGGAATAGC CATGACCGACACCACACCGTATGGTCAGCAAAGAGTTTTCAAGGAAAAAGTGGACACTAGGGTGCCAGACCCCC- AAGAAGGC ACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTTGTGGAAAGAGCTAGGCAAACACAAACGGCCACGAGTCTG- TACCAAAGA AGAGTTCATCAACAAGGTTCGTAGCAATGCAGCATTAGGGGCAATATTTGAAGAGGAAAAAGAGTGGAAGACTG- CAGTGGA AGCTGTGAACGATCCAAGGTTCTGGGCTCTAGTGGATAAGGAAAGAGAGCACCACCTGAGAGGAGAGTGCCAGA- GTTGTGT GTACAACATGATGGGAAAAAGAGAAAAGAAACAAGGGGAATTTGGAAAGGCCAAGGGCAGCCGCGCCATCTGGT- ATATGTG GCTAGGGGCTAGATTTCTAGAGTTCGAAGCCCTTGGATTCTTGAACGAGGATCACTGGATGGGGAGAGAGAACT- CAGGAGGT GGTGTTGAAGGGCTGGGATTACAAAGACTCGGATATGTCCTAGAAGAGATGAGTCGTATACCAGGAGGAAGGAT- GTATGCA GATGACACTGCTGGCTGGGACACCCGCATCAGCAGGTTTGATCTGGAGAATGAAGCTCTAATCACCAACCAAAT- GGAAAAAG GGCACAGGGCCTTGGCATTGGCCATAATCAAGTACACATACCAAAACAAAGTGGTAAAGGTCCTTAGACCAGCT- GAAAAAGG GAAAACAGTTATGGACATTATTTCGAGACAAGACCAAAGGGGGAGCGGACAAGTTGTCACTTACGCTCTTAACA- CATTTACCA ACCTAGTGGTGCAACTCATTCGGAATATGGAGGCTGAGGAAGTTCTAGAGATGCAAGACTTGTGGCTGCTGCGG- AGGTCAGA GAAAGTGACCAACTGGTTGCAGAGCAACGGATGGGATAGGCTCAAACGAATGGCAGTCAGTGGAGATGATTGCG- TTGTGAA GCCAATTGATGATAGGTTTGCACATGCCCTCAGGTTCTTGAATGATATGGGAAAAGTTAGGAAGGACACACAAG- AGTGGAAA CCCTCAACTGGATGGGACAACTGGGAAGAAGTTCCGTTTTGCTCCCACCACTTCAACAAGCTCCATCTCAAGGA- CGGGAGGTC CATTGTGGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCCCGCGTCTCTCCAGGGGCGGGATGGAGCA- TCCGGGAG ACTGCTTGCCTAGCAAAATCATATGCGCAAATGTGGCAGCTCCTTTATTTCCACAGAAGGGACCTCCGACTGAT- GGCCAATGCC ATTTGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGGAGAACTACCTGGTCAATCCATGGAAAGGGAGAATG- GATGACCAC TGAAGACATGCTTGTGGTGTGGAACAGAGTGTGGATTGAGGAGAACGACCACATGGAAGACAAGACCCCAGTTA- CGAAATG GACAGACATCCCCTATTTGGGAAAAAGGGAAGACTTGTGGTGTGGATCTCTCATAGGGCACAGACCGCGCACCA- CCTGGGCT GAGAACATTAAAAACACAGTCAACATGGTGCGCAGGATCATAGGTGATGAAGAAAAGTACATGGACTACCTATC- CACCCAAG TTCGCTACTTGGGTGAAGAAGGGTCTACACCTGGAGTGCTGTAAGCACCAGTCTTAATGTTGTCAGGCCTGCTA- GTCAGCCAC AGCTTGGGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGAAGCTGGGAAACCAAGCCTATAGTCAGGCCGAGA- ACGCCATG GCACGGAAGAAGCCATGCTGCCTGTGAGCCCCTCAGAGGACACTGAGTCAAAAAACCCCACGCGCTTGGAGGCG- CAGGATG GGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAACTGGAGATCAGCTGTGGATCTCCAGAA- GAGGGACT AGTGGTTAGAGGAG KU744693.1 Zika virus isolate VE_Ganxian, China, complete genome SEQ ID NO: 10 GTTGTTACTGTTGCTGACTCAGACTGCGACAGTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTT- TATTTGGATT TGGAAACGAGAGTTTCTGGTCATGAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATATGCTAAA- ACGCGGAG TAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGTCATGGGCCCATCAGG- ATGGTCTTG GCAATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAGATGGGGTTCAGTGGG- GAAAAAAGA TGCTATGGAAATAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGCTGAGAATAATCAATGCTAGGAAGGAGA- AGAAGAGA CGAGGCGCAGATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGCGGAGGTCACTAGACG- TGGGAGTG CATACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAAG- TGTTATATAC AGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGGTGGAA- CCAGATGA CGTCGATTGTTGGTGCAACACGACGTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAAAAGGTGAAGCAC- GGAGATCTA GAAGAGCTGTGACGCTCCCTTCCCATTCCACTAGGAAGCTGCAAACGCGGTCGCAAACCTGGTTGGAATCAAGA- GAATACACA AAGCACTTGATTAGAGTCGAAAATTGGATATTCAGGAACCCTGGCTTCGCGTTAGCAGCAGCTGCCATCGCTTG- GCTTTTGGG AAGCTCAACGAGCCAAAAAGTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCA- TAGGAGTCA GCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTGATGTTGTCTTGGAACATGGAGGTTGTGTC- ACCGCAAT GGCACAGGACAAACCGACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGTAAGATCCT- ACTGCTAT GAGGCATCAATATCAGACATGGCTTCGGACAGCCGCTGCCCAACACAAGGTGAAGCCTACCTTGACAAGCAATC- AGACACTCA ATATGTTTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGTCTGG- TGACATGC GCTAAGTTTGCATGCTCCAAGAAAATGACCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAATGCT- GTCAGTTC ATGGCTCCCAGCACAGTGGGATGCTCGTTAATGACACAGGACATGAAACTGATGAGAATAGAGCGAAGGTTGAG- ATAACGCC CAATTCACCAAGAGCCGAAGCCACCCTGGGGGGTTTTGGAAGCCTAGGACTTGATTGTGAACCGAGGACAGGCC- TTGACTTTT CAGATTTGTATTACTTGACTATGAATAACAAGCACTGGTTGGCTCACAAGGAGTGGTTCCACGACATTCCATTA- CCTTGGCACG CTGGGGCAGCCACCGGAACTCCACACTGGAACAACAAAGAAGCACTGGTAGAGTTCAAGGACGCACATGCCAAA- AGGCAAAC TGTCGTGGTTCTAGGGAGTCAAGAAGGAGCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGGATG- GTGCAAAG GGAAGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATAAACTTAGATTGAAGGGCGTGTCATACTC- CTTGTGTAC CGCAGCGTTCACATTCACCAAGATCCCGGCTGAAACAGTGGACGGGACAGTCACAGTGGAGGGACAGTACGGAG- GGACAGA TGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGACATGCAGACTCTGACCCCAGTTGGGAGGTTGATAACCG- CTAACCCCG TAATCACTGAAAGCACTGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACATTGTC- ATAGGAGTCG GGGAGAAGAAGATCACCCACCACTGGCACAGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGAGA- GGTGCCA AGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGTTGGAGGCGCTCTCAACTCATTGGGCAAG- GGCATCCA TCAAATTATTGGAGCAGCTTTCAAATCATTGTTTGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGGACGT- TGCTGATGTG GTTGGGTCTGAACACAAAGAATGGATCTATTTCCCTTATGTGCTTGGCCTTAGGGGGAGTGTTGATCTTCTTAT- CCACAGCCGT CTCAGGTGGTGTGGGGTGCTCGGTGGACTTCTCAAAGAAGGAGACGAGATGCGGTACAGGGGTGTTCGTCTATA- ACGATGTT GAAGCCTGGAGGGACAGGTACAAGTACCATCCTGACTCCCCCCGTAGATTGGCAGCAGCAGTCAAGCAAGCCTG- GGAAGATG GTATCTGCGGGATCTCCTCTGTTTCAAGAATGGAAAACATCATGTGGAGATCAGTAGAAGGGGAGCTCAACGCA- ATCCTGGAA GAGAATGGAGTTCAACTGACGGTCGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATTGCC- CGTGCCTG TGAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCGTACTTCGTCAGAGCAGCAAAGACAAATAACAGC- TTTGTCGT

GGATGGTGACACACTGAAGGAATGCCCACTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAGGATCATGGGT- TCGGGGTA TTTCACACTAGTGTCTGGCTCAAGGTTAGAGAAGACTATTGGTTAGAGTGTGATCCAGCCGTTATTGGAACAGC- TGTTAAGGG AAAGGAGGCTGTACACAGTGATCTAGGCTACTGGATTGAGAGTGAGAAGAATGACACATGGTGGCTGAAGAGGG- CCCATCT GATCGAGATGAAAACATGTGAATGGCCAAAGTCCCACACATTGTGGACAGATGGAATAGAAGAGAGTGATCTGA- TCATACCC AAGTCTTTAGCTGGGCCACTCAGCCATCACAATGCCAGAGAGGGCTACAGGACCCAAATGAAAGGGCCATGGCA- CAGTGAAG AGCTTGAAATTCGGTTTGAGGAATGCCCAGGCACTAAGGTCCACGTGGAGGAAACATGTGGAACAAGAGGACCA- TCTCTGAG ATCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGTGCTCCAGGGAGTGCACAATGCCCCCACTGTCCTTCC- AGGCTAAA GATGGCTGTTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAAGCAACTTAGTAAGGTCAATGGTGAC- TGCAGGA TCAACTGATCACATGGATCACTTCTCCCTTGGAGTGCTTGTGATTCTGCTCATGGTGCAGGAAGGGCTGAAGAA- GAGAATGAC CACAAAGATCATCATAAGCACATCAATGGCAGTGCTGGTAGCTATGATCCTGGGAGGATTTTCAATGAGTGACC- TGGCTAAGC TTGCAATTTTGATGGGTGCCACCTTCGCGGAAATGAACACTGGAGGAGATGTAGCTCATCTGGCGCTGATAGCG- GCATTCAAA GTCAGACCAGCGTTGCTGGTATCTTTCATCTTCAGAGCTAATTGGACACCCCGTGAAAGCATGCTGCTGGCCTT- GGCCTCGTGT CTTTTGCAAACTGCGATCTCCGCCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGGTT- GGCAATACGA GCGATGGTTGTTCCACGCACTGATAACATCACCTTAGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCAC- ACTGCTTGTG GCGTGGAGAGCAGGCCTTGCTACTTGCGGGGGGTTTATGCTCCTCTCTCTGAAGGGAAAAGGCAGTGTGAAGAA- GAACTTAC CATTTGTCATGGCCCTGGGACTAACCGCTGTGAGGCTGGTCGACCCCATCAACGTGGTGGGACTGCTGTTGCTC- ACAAGGAGT GGGAAGCGGAGCTGGCCCCCTAGCGAAGTACTCACAGCTGTTGGCCTGATATGCGCATTGGCTGGAGGGTTCGC- CAAGGCAG ATATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAGGAAAGAGTGTG- GACATGTA CATTGAAAGAGCAGGTGACATCACATGGGAAAAAGATGCGGAAGTCACTGGAAACAGTCCCCGGCTCGATGTGG- CGCTAGAT GAGAGTGGTGATTTCTCCCTGGTGGAGGATGACGGTCCCCCCATGAGAGAGATCATACTCAAGGTGGTCCTGAT- GACCATCTG TGGCATGAACCCAATAGCCATACCCTTTGCAGCTGGAGCGTGGTACGTATACGTGAAGACTGGAAAAAGGAGTG- GTGCTCTAT GGGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCACAGATGGAGTGTACAGAGTAATGACTCGCAGA- CTGCTAG GTTCAACACAAGTTGGAGTGGGAGTTATGCAAGAGGGGGTCTTTCACACTATGTGGCACGTCACAAAAGGATCC- GCGCTGAG AAGCGGTGAAGGGAGACTTGATCCATACTGGGGAGATGTCAAGCAGGATCTGGTGTCATACTGTGGTCCATGGA- AGCTAGAT GCCGCCTGGGACGGGCACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGAGAGCGAGGAACATCCAGAC- TCTGCCC GGAATATTTAAGACAAAGGATGGGGACATTGGAGCGGTTGCACTGGATTACCCAGCAGGAACTTCAGGATCTCC- AATCCTAG ACAAGTGTGGGAGAGTGATAGGACTTTATGGCAATGGGGTCGTGATCAAAAATGGGAGTTATGTTAGTGCCATC- ACCCAAGG GAGGAGGGAGGAAGAGACTCCTGTTGAGTGCTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTAACTGTCTTAG- ACTTGCAT CCTGGAGCTGGGAAAACCAGGAGAGTTCTTCCTGAAATAGTCCGTGAAGCCATAAAAACAAGACTCCGTACTGT- GATCTTGGC TCCAACCAGGGTTGTCGCTGCTGAAATGGAGGAGGCCCTTAGAGGGCTTCCAGTGCGTTATATGACAACAGCAG- TCAATGTCA CCCACTCTGGAACAGAAATCGTCGACTTAATGTGCCATGCCACCTTCACTTCACGTCTACTACAGCCAATTAGA- GTCCCCAACTA TAATCTGTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAAGAGGATACATTTCAACAA- GGGTTGAGA TGGGCGAGGCGGCTGCCATCTTCATGACCGCCACGCCACCAGGAACCCGTGACGCATTTCCGGACTCCAACTCA- CCAATTATG GACACCGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTCAGGCTTTGATTGGGTGACGGAGTATTCTGGAAAAAC- AGTTTGGT TTGTTCCACGCGTGAGGAACGGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAACGGGTCATACAGCTC- AGCAGAAA GACTTTTGAGACAGAGTTCCAGAAAACAAAACATCAAGAGTGGGACTTTGTCGTGACAACTGACATTTCAGAGA- TGGGCGCCA ACTTTAAAGCTGACCGTGTCATAGATTCCAGGAGATGCCTAAAGCCGGTCATACTTGGTGGCGAGAGAGTCATT- CTGGCTGGA CCCATGCCTGTCACACATGCCAGCGCTGCCCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGGAGA- TGAGTATC TGTATGGAGGTGGGTGCGCAGAGACTGACGAAGACCATGCACACTGGCTTGAAGCAAGAATGCTCCTTGACAAT- ATTTACCTC CAAGATGGCCTCATAGCCTCGCTCTATCGACCTGAGGCCGACAAAGTAGCAGCCATTGAGGGAGAGTTCAAGCT- TAGGACGG AGCAAAGGAAGACCTTTGTGGAACTCATGAAAAGAGGAGATCTTCCTGTTTGGCTGGCCTATCAGGTTGCATCT- GCCGGAATA ACCTACACAGATAGAAGATGGTGCTTTGATGGCACGACCAACAACACCATAATGGAAGACAGTGTGCCGGCAGA- GGTGTGGA CCAGACACGGAGAGAAAAGAGTGCTCAAACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCCTG- AAGTCATT CAAGGAGTTTGCCGCTGGGAAAAGAGGAGCGGCTTTTGGAGTGATGGAAGCCCTGGGAACACTGCCAGGACACA- TGACAGA GAGATTCCAGGAAGCCATTGACAACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGCCTTACAAAGCCG- CGGCGGCC CAATTGCCGGAGACCCTAGAGACCATTATGCTTTTGGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTTTTCGT- CTTGATGAGG AACAAGGGCATAGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGCGCATGGCTCATGTGGCTCTCGGA- AATTGAGC CAGCCAGAATTGCATGTGTCCTCATTGTTGTGTTCCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAA- AGATCTCCCC AGGACAACCAAATGGCCATCATCATCATGGTAGCAGTAGGTCTTCTGGGCTTGATTACCGCCAATGAACTCGGA- TGGTTGGAG AGAACAAAGAGTGACCTAAGCCATCTAATGGGAAGGAGAGAGGAGGGGGCAACCATGGGATTCTCAATGGACAT- TGACCTG CGGCCAGCCTCAGCTTGGGCCATCTATCCTGCCTTGACATCTTTCATTACCCCAGCCGTCCAACATGCAGTGAC- CACTTCATACA ACAACTACTCCTTAATGGCGATGGCCACGCAAGCTGGAGTGTTGTTTGGTATGGGCAAAGGGATGCCATTCTAC- GCATGGGAC TTTGGAGTCCCGCTGCTAATGATAGGTTGCTACTCACAATTAACGCCCCTGACCCTAATAGTGGCCATCATTTT- GCTCGTGGCG CACTACATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCGTGCTGCCCAGAAGAGAACGGCAGCTGGCAT- CATGAAGA ACCCTGTTGTGGAGGGAATAGTGGTGACTGACATTGACACAATGACAATTGACCCCCAAGTGGAGAAAAAGATG- GGACAGGT GCTACTCATGGCAGTAGCCGTCTCCAGCGCCATACTGTCGAGGACCGCCTGGGGGTGGGGGGAGGCTGGGGCCC- TGATCACA GCCGCAACTTCCACTTTGTGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCTACAGCCACCTCACTGTGTAA- CATTTTTAGG GGAAGTTACTTGGCTGGAGCTTCTCTAATCTACACAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGG- TGGAACAG GAGAGACCCTGGGAGAGAAATGGAAGGCCCGCTTGAACCAGATGTCGGCCCTGGAGTTCTACTCCTACAAAAAG- TCAGGCAT CACCGAGGTGTGCAGAGAAGAGGCCCGCCGCGCCCTCAAGGACGGTGTGGCAACGGGAGGCCATGCTGTGTCCC- GAGGAAG TGCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCTGCAGCCCTATGGAAAGGTCATTGATCTTGGATGTGGCA- GAGGGGG CTGGAGTTACTACGCCGCCACCATCCGCAAAGTTCAAGAAGTGAAAGGATACACAAAAGGAGGCCCTGGTCATG- AAGAACCC GTGTTGGTGCAAAGCTATGGGTGGAACATAGTCCGTCTTAAGAGTGGGGTGGACGTCTTTCATATGGCGGCTGA- GCCGTGTG ACACGTTGCTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAAGCACGGACGCTCAGAGTCCTC- TCCATGGTG GGGGATTGGCTTGAAAAAAGACCAGGAGCCTTTTGTATAAAAGTGTTGTGCCCATACACCAGCACTATGATGGA- AACCCTGGA GCGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGAGTGCCACTCTCCCGCAACTCTACACATGAGATGTACT- GGGTCTCT GGAGCGAAAAGCAACACCATAAAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCGCATGGACGGGCCTAG- GAGGCCA GTGAAATATGAGGAGGATGTGAATCTCGGCTCTGGCACGCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACAT- GAAGATCA TTGGTAACCGCATTGAAAGGATCCGCGCTGAGAAAGCGGAAACGTGGTTCTTTGACGAGAACCACCCATATAGG- ACATGGGC TTACCATGGAAGCTATGATGCCGCCACACAAGGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGT- CAAAACCCT GGGATGTGGTGACTGGAGTCACAGGAATAGCCATGACCGACACCACACCGTATGGTCAGCAAAGAGTTTTCAAG- GAAAAAGT GGACACTAGGGTGCCAGACCCCCAAGAAGGCACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTTGTGGAAAG- AGCTAGGC AAACACAAACGGCCACGAGTCTGTACCAAAGAAGAGTTCATCAACAAGGTTCGTAGCAATGCAGCATTAGGGGC- AATATTTGA AGAGGAAAAAGAGTGGAAGACTGCAGTGGAAGCTGTGAACGATCCAAGGTTCTGGGCTCTAGTGGACAAGGAAA- GAGAGCA CCACCTGAGAGGAGAGTGCCAGAGTTGTGTGTACATCACAATGGGAAAAAGAGAAAAGAAACAAGGGGAATTTG- GAAAGGC CAAGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAGATTTCTAGAGTTCGAAGCCCTTGGATTCTTGA- ACGAGGATC ACTGGATGGGGAGAGAGAACTCAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGATATGTCCTAGAA- GAGATGA GTCGCATACCAGGAGGAAGGATGTATGCAGATGACACTGCTGGCTGGGACACCCGCATCAGCAGGTTTGATCTG- GAGAATGA AGCTCTAATCACCAACCAAATGGAGAAAGGGCACAGGGCCTTGGCATTGGCCATAATCAAGTACACATACCAAA- ACAAAGTG GTAAAGGTCCTTAGACCAGCTGAAAAAGGGAAGACAGTTATGGACATTATTTCGAGACAAGACCAAAGGGGGAG- CGGACAA GTTGTCACTTACGCTCTCAACACATTTACCAACCTAGTGGTGCAACTCATTCGGAATATGGAGGCTGAGGAAGT- TCTAGAGATG CAAGACTTGTGGCTGCTGCGGAGGTCAGAGAAAGTGACCAACTGGTTGCAGAGCAACGGATGGGATAGGCTCAA- ACGAATG GCGGTCAGTGGAGATGATTGCGTTGTGAAACCAATTGATGATAGGTTTGCACATGCCCTCAGGTTCTTGAATGA- TATGGGAAA AGTTAGGAAGGACACACAAGAGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAAGTTCCCTTCTGCTCCC- ACCACTTCA

ACAAGCTCCATCTCAAGGACGGGAGGTCCATTGTGGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCC- CGCGTCTCT CCAGGGGCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGCAAATGTGGCAGCTCCTTTA- TTTCCACAG AAGGGACCTCCGACTGATGGCCAATGCCATTTGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGGAGAACTA- CCTGGTCAAT CCATGGAAAGGGAGAATGGATGACCACTGAAGACATGCTTGTGGCGTGGAACAGAGTGTGGATTGAGGAGAACG- ACCACAT GGAAGACAAGACCCCAGTCACGAAATGGACAGACATTCCCTATTTGGGAAAAAGGGAAGACTTGTGGTGTGGAT- CTCTCATA GGGCACAGACCGCGCACCACCTGGGCTGAGAACATTAAAAACACAGTCAACATGGTGCGCAGGATCATAGGTGA- TGAAGAAA AGTACATGGACTACCTATCCACCCAAGTTCGCTACTTGGGTGAAGAAGGGTCTACACCTGGAGTGCTGTAAGCA- CCAATCTTA ATGTTGTCAGGCCTGCTAGTCAGCCACAGCTTGGGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGAAGCTGG- GAAACCAA GCCTATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTGAGCCCCTCAGAGGACACTGAG- TCAAAAAA CCCCACGCGCTTGGAGGCGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAAC- TGGAGATC AGCTGTGGATCTCCAGAAGAGGGACTAGTGGTTAGAGGAGA LC002520.1 Zika virus genomic RNA, strain: MR766-NIID, Uganda, complete genome SEQ ID NO: 11 AGTTGTTGATCTGTGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTT- TAATTTGGA TTTGGAAACGAGAGTTTCTGGTCATGAAAAACCCAAAGAAGAAATCCGGAGGATTCCGGATTGTCAATATGCTA- AAACGCGG AGTAGCCCGTGTAAACCCCTTGGGAGGTTTGAAGAGGTTGCCAGCCGGACTTCTGCTGGGTCATGGACCCATCA- GAATGGTTT TGGCGATACTAGCCTTTTTGAGATTTACAGCAATCAAGCCATCACTGGGCCTTATCAACAGATGGGGTTCCGTG- GGGAAAAAA GAGGCTATGGAAATAATAAAGAAGTTCAAGAAAGATCTTGCTGCCATGTTGAGAATAATCAATGCTAGGAAAGA- GAGGAAGA GACGTGGCGCAGACACCAGCATCGGAATCATTGGCCTCCTGCTGACTACAGCCATGGCAGCAGAGATCACTAGA- CGCGGGAG TGCATACTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAGTGAACA- AGTGCCACG TACAGATCATGGACCTCGGGCACATGTGTGACGCCACCATGAGTTATGAGTGCCCTATGCTGGATGAGGGAGTG- GAACCAGA TGATGTCGATTGCTGGTGCAACACGACATCAACTTGGGTTGTGTACGGAACCTGTCATCACAAAAAAGGTGAGG- CACGGCGAT CTAGAAGAGCCGTGACGCTCCCTTCTCACTCTACAAGGAAGTTGCAAACGCGGTCGCAGACCTGGTTAGAATCA- AGAGAATAC ACGAAGCACTTGATCAAGGTTGAAAACTGGATATTCAGGAACCCCGGGTTTGCGCTAGTGGCCGTTGCCATTGC- CTGGCTTTT GGGAAGCTCGACGAGCCAAAAAGTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGTATCAGGT- GCATTGGAG TCAGCAATAGAGACTTCGTGGAGGGCATGTCAGGTGGGACCTGGGTTGATGTTGTCTTGGAACATGGAGGCTGC- GTTACCGT GATGGCACAGGACAAGCCAACAGTTGACATAGAGTTGGTCACGACGACGGTTAGTAACATGGCCGAGGTAAGAT- CCTATTGC TACGAGGCATCGATATCGGACATGGCTTCGGACAGTCGTTGCCCAACACAAGGTGAAGCCTACCTTGACAAGCA- ATCAGACAC TCAATATGTCTGCAAAAGAACATTAGTGGACAGAGGTTGGGGAAACGGTTGTGGACTTTTTGGCAAAGGGAGCT- TGGTGACA TGTGCCAAGTTTACGTGTTCTAAGAAGATGACCGGGAAGAGCATTCAACCGGAAAATCTGGAGTATCGGATAAT- GCTATCAGT GCATGGCTCCCAGCATAGCGGGATGACTGTCAATGATATAGGATATGAAACTGACGAAAATAGAGCGAAAGTCG- AGGTTACG CCTAATTCACCAAGAGCGGAAGCAACCTTGGGAGGCTTTGGAAGCTTAGGACTTGACTGTGAACCAAGGACAGG- CCTTGACTT TTCAGATCTGTATTACCTGACCATGAACAATAAGCATTGGTTGGTGCACAAAGAGTGGTTTCATGACATCCCAT- TGCCTTGGCA TGCTGGGGCAGACACTGGAACTCCACACTGGAACAACAAAGAGGCATTGGTAGAATTCAAGGATGCCCACGCCA- AGAGGCAA ACCGTCGTCGTTCTGGGGAGCCAGGAAGGAGCCGTTCACACGGCTCTCGCTGGAGCTCTAGAGGCTGAGATGGA- TGGTGCAA AGGGAAAGCTGTTCTCTGGCCATTTGAAATGCCGCCTAAAAATGGACAAGCTTAGATTGAAGGGCGTGTCATAT- TCCTTGTGC ACTGCGGCATTCACATTCACCAAGGTCCCAGCTGAAACACTGCATGGAACAGTCACAGTGGAGGTGCAGTATGC- AGGGACAG ATGGACCCTGCAAGATCCCAGTCCAGATGGCGGTGGACATGCAGACCCTGACCCCAGTTGGAAGGCTGATAACC- GCCAACCC CGTGATTACTGAAAGCACTGAGAACTCAAAGATGATGTTGGAGCTTGACCCACCATTTGGGGATTCTTACATTG- TCATAGGAG TTGGGGACAAGAAAATCACCCACCACTGGCATAGGAGTGGTAGCACCATCGGAAAGGCATTTGAGGCCACTGTG- AGAGGCGC CAAGAGAATGGCAGTCCTGGGGGATACAGCCTGGGACTTCGGATCAGTCGGGGGTGTGTTCAACTCACTGGGTA- AGGGCATT CACCAGATTTTTGGAGCAGCCTTCAAATCACTGTTTGGAGGAATGTCCTGGTTCTCACAGATCCTCATAGGCAC- GCTGCTAGTG TGGTTAGGTTTGAACACAAAGAATGGATCTATCTCCCTCACATGCTTGGCCCTGGGGGGAGTGATGATCTTCCT- CTCCACGGCT GTTTCTGCTGACGTGGGGTGCTCAGTGGACTTCTCAAAAAAGGAAACGAGATGTGGCACGGGGGTATTCATCTA- TAATGATGT TGAAGCCTGGAGGGACCGGTACAAGTACCATCCTGACTCCCCCCGCAGATTGGCAGCAGCAGTCAAGCAGGCCT- GGGAAGAG GGGATCTGTGGGATCTCATCCGTTTCAAGAATGGAAAACATCATGTGGAAATCAGTAGAAGGGGAGCTCAATGC- TATCCTAGA GGAGAATGGAGTTCAACTGACAGTTGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAAAGATTGC- CAGTGCCT GTGAATGAGCTGCCCCATGGCTGGAAAGCCTGGGGGAAATCGTATTTTGTTAGGGCGGCAAAGACCAACAACAG- TTTTGTTGT CGACGGTGACACACTGAAGGAATGTCCGCTTGAGCACAGAGCATGGAATAGTTTTCTTGTGGAGGATCACGGGT- TTGGAGTC TTCCACACCAGTGTCTGGCTTAAGGTCAGAGAAGATTACTCATTAGAATGTGACCCAGCCGTCATAGGAACAGC- TGTTAAGGG AAGGGAGGCCGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACACATGGAGGCTGAAGAGGG- CCCACCT GATTGAGATGAAAACATGTGAATGGCCAAAGTCTCACACATTGTGGACAGATGGAGTAGAAGAAAGTGATCTTA- TCATACCCA AGTCTTTAGCTGGTCCACTCAGCCACCACAACACCAGAGAGGGTTACAGAACCCAAGTGAAAGGGCCATGGCAC- AGTGAAGA GCTTGAAATCCGGTTTGAGGAATGTCCAGGCACCAAGGTTTACGTGGAGGAGACATGCGGAACTAGAGGACCAT- CTCTGAGA TCAACTACTGCAAGTGGAAGGGTCATTGAGGAATGGTGCTGTAGGGAATGCACAATGCCCCCACTATCGTTTCG- AGCAAAAG ACGGCTGCTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAGAGCAACTTAGTGAGGTCAATGGTGACA- GCGGGGT CAACCGATCATATGGACCACTTCTCTCTTGGAGTGCTTGTGATTCTACTCATGGTGCAGGAGGGGTTGAAGAAG- AGAATGACC ACAAAGATCATCATGAGCACATCAATGGCAGTGCTGGTAGTCATGATCTTGGGAGGATTTTCAATGAGTGACCT- GGCCAAGCT TGTGATCCTGATGGGTGCTACTTTCGCAGAAATGAACACTGGAGGAGATGTAGCTCACTTGGCATTGGTAGCGG- CATTTAAAG TCAGACCAGCCTTGCTGGTCTCCTTCATTTTCAGAGCCAATTGGACACCCCGTGAGAGCATGCTGCTAGCCCTG- GCTTCGTGTC TTCTGCAAACTGCGATCTCTGCTCTTGAAGGTGACTTGATGGTCCTCATTAATGGATTTGCTTTGGCCTGGTTG- GCAATTCGAGC AATGGCCGTGCCACGCACTGACAACATCGCTCTACCAATCTTGGCTGCTCTAACACCACTAGCTCGAGGCACAC- TGCTCGTGGC ATGGAGAGCGGGCCTGGCTACTTGTGGAGGGATCATGCTCCTCTCCCTGAAAGGGAAAGGTAGTGTGAAGAAGA- ACCTGCCA TTTGTCATGGCCCTGGGATTGACAGCTGTGAGGGTAGTAGACCCTATTAATGTGGTAGGACTACTGTTACTCAC- AAGGAGTGG GAAGCGGAGCTGGCCCCCTAGTGAAGTTCTCACAGCCGTTGGCCTGATATGTGCACTGGCCGGAGGGTTTGCCA- AGGCAGAC ATTGAGATGGCTGGACCCATGGCTGCAGTAGGCTTGCTAATTGTCAGCTATGTGGTCTCGGGAAAGAGTGTGGA- CATGTACAT TGAAAGAGCAGGTGACATCACATGGGAAAAGGACGCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCAC- TGGATGA GAGTGGTGATTTCTCCTTGGTAGAGGAAGATGGTCCACCCATGAGAGAGATCATACTTAAGGTGGTCCTGATGG- CCATCTGTG GCATGAACCCAATAGCTATACCTTTTGCTGCAGGAGCGTGGTATGTGTATGTGAAGACTGGGAAAAGGAGTGGC- GCCCTCTG GGACGTGCCTGCTCCCAAAGAAGTGAAGAAAGGAGAGACCACAGATGGAGTGTACAGAGTGATGACTCGCAGAC- TGCTAGG TTCAACACAGGTTGGAGTGGGAGTCATGCAAGAGGGAGTCTTCCACACCATGTGGCACGTTACAAAAGGAGCCG- CACTGAGG AGCGGTGAGGGAAGACTTGATCCATACTGGGGGGATGTCAAGCAGGACTTGGTGTCATACTGTGGGCCTTGGAA- GTTGGATG CAGCTTGGGATGGACTCAGCGAGGTACAGCTTTTGGCCGTACCTCCCGGAGAGAGGGCCAGAAACATTCAGACC- CTGCCTGG AATATTCAAGACAAAGGACGGGGACATCGGAGCAGTTGCTCTGGACTACCCTGCAGGGACCTCAGGATCTCCGA- TCCTAGAC AAATGTGGAAGAGTGATAGGACTCTATGGCAATGGGGTTGTGATCAAGAATGGAAGCTATGTTAGTGCTATAAC- CCAGGGAA AGAGGGAGGAGGAGACTCCGGTTGAATGTTTCGAACCCTCGATGCTGAAGAAGAAGCAGCTAACTGTCTTGGAT- CTGCATCC AGGAGCCGGAAAAACCAGGAGAGTTCTTCCTGAAATAGTCCGTGAAGCCATAAAAAAGAGACTCCGGACAGTGA- TCTTGGCA CCAACTAGGGTTGTCGCTGCTGAGATGGAGGAGGCCTTGAGAGGACTTCCGGTGCGTTACATGACAACAGCAGT- CAACGTCA CCCATTCTGGGACAGAAATCGTTGATTTGATGTGCCATGCCACTTTCACTTCACGCTTACTACAACCCATCAGA- GTCCCTAATTA CAATCTCTACATCATGGATGAAGCCCACTTCACAGACCCCTCAAGTATAGCTGCAAGAGGATATATATCAACAA- GGGTTGAAAT GGGCGAGGCGGCTGCCATTTTTATGACTGCCACACCACCAGGAACCCGTGATGCGTTTCCTGACTCTAACTCAC- CAATCATGGA CACAGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTCAGGCTTTGATTGGGTGACAGACCATTCTGGGAAAACAG- TTTGGTTC GTTCCAAGCGTGAGAAACGGAAATGAAATCGCAGCCTGTCTGACAAAGGCTGGAAAGCGGGTCATACAGCTCAG- CAGGAAG ACTTTTGAGACAGAATTTCAGAAAACAAAAAATCAAGAGTGGGACTTTGTCATAACAACTGACATCTCAGAGAT- GGGCGCCAA CTTCAAGGCTGACCGGGTCATAGACTCTAGGAGATGCCTAAAACCAGTCATACTTGATGGTGAGAGAGTCATCT- TGGCTGGGC

CCATGCCTGTCACGCATGCTAGTGCTGCTCAGAGGAGAGGACGTATAGGCAGGAACCCTAACAAACCTGGAGAT- GAGTACAT GTATGGAGGTGGGTGTGCAGAGACTGATGAAGGCCATGCACACTGGCTTGAAGCAAGAATGCTTCTTGACAACA- TCTACCTCC AGGATGGCCTCATAGCCTCGCTCTATCGGCCTGAGGCCGATAAGGTAGCCGCCATTGAGGGAGAGTTTAAGCTG- AGGACAGA GCAAAGGAAGACCTTCGTGGAACTCATGAAGAGAGGAGACCTTCCCGTCTGGCTAGCCTATCAGGTTGCATCTG- CCGGAATAA CTTACACAGACAGAAGATGGTGCTTTGATGGCACAACCAACAACACCATAATGGAAGACAGCGTACCAGCAGAG- GTGTGGAC AAAGTATGGAGAGAAGAGAGTGCTCAAACCGAGATGGATGGATGCTAGGGTCTGTTCAGACCATGCGGCCCTGA- AGTCGTTC AAAGAATTCGCCGCTGGAAAAAGAGGAGCGGCTTTGGGAGTAATGGAGGCCCTGGGAACACTGCCAGGACACAT- GACAGAG AGGTTTCAGGAAGCCATTGACAACCTCGCCGTGCTCATGCGAGCAGAGACTGGAAGCAGGCCTTATAAGGCAGC- GGCAGCCC AACTGCCGGAGACCCTAGAGACCATTATGCTCTTAGGTTTGCTGGGAACAGTTTCACTGGGGATCTTCTTCGTC- TTGATGCGGA ATAAGGGCATCGGGAAGATGGGCTTTGGAATGGTAACCCTTGGGGCCAGTGCATGGCTCATGTGGCTTTCGGAA- ATTGAACC AGCCAGAATTGCATGTGTCCTCATTGTTGTGTTTTTATTACTGGTGGTGCTCATACCCGAGCCAGAGAAGCAAA- GATCTCCCCA AGATAACCAGATGGCAATTATCATCATGGTGGCAGTGGGCCTTCTAGGTTTGATAACTGCAAACGAACTTGGAT- GGCTGGAAA GAACAAAAAATGACATAGCTCATCTAATGGGAAGGAGAGAAGAAGGAGCAACCATGGGATTCTCAATGGACATT- GATCTGCG GCCAGCCTCCGCCTGGGCTATCTATGCCGCATTGACAACTCTCATCACCCCAGCTGTCCAACATGCGGTAACCA- CTTCATACAAC AACTACTCCTTAATGGCGATGGCCACACAAGCTGGAGTGCTGTTTGGCATGGGCAAAGGGATGCCATTTTATGC- ATGGGACCT TGGAGTCCCGCTGCTAATGATGGGTTGCTATTCACAATTAACACCCCTGACTCTGATAGTAGCTATCATTCTGC- TTGTGGCGCA CTACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCCAGAAAAGGACAGCAGCTGGCATCA- TGAAGAAT CCCGTTGTGGATGGAATAGTGGTAACTGACATTGACACAATGACAATAGACCCCCAGGTGGAGAAGAAGATGGG- ACAAGTGT TACTCATAGCAGTAGCCATCTCCAGTGCTGTGCTGCTGCGGACCGCCTGGGGATGGGGGGAGGCTGGAGCTCTG- ATCACAGC AGCGACCTCCACCTTGTGGGAAGGCTCTCCAAACAAATACTGGAACTCCTCTACAGCCACCTCACTGTGCAACA- TCTTCAGAGG AAGCTATCTGGCAGGAGCTTCCCTTATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTG- GGACGGGA GAGACTCTGGGAGAGAAGTGGAAAGCTCGTCTGAATCAGATGTCGGCCCTGGAGTTCTACTCTTATAAAAAGTC- AGGTATCAC TGAAGTGTGTAGAGAGGAGGCTCGCCGTGCCCTCAAGGATGGAGTGGCCACAGGAGGACATGCCGTATCCCGGG- GAAGTGC AAAGCTCAGATGGTTGGTGGAGAGAGGATATCTGCAGCCCTATGGGAAGGTTGTTGACCTCGGATGTGGCAGAG- GGGGCTG GAGCTATTATGCCGCCACCATCCGCAAAGTGCAGGAGGTGAGAGGATACACAAAGGGAGGTCCCGGTCATGAAG- AACCCATG CTGGTGCAAAGCTATGGGTGGAACATAGTTCGTCTCAAGAGTGGAGTGGACGTCTTCCACATGGCGGCTGAGCC- GTGTGACA CTCTGCTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAGACACGAACACTCAGAGTGCTCTCT- ATGGTGGGG GACTGGCTTGAAAAAAGACCAGGGGCCTTCTGTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAAC- CATGGAGC GACTGCAACGTAGGCATGGGGGAGGATTAGTCAGAGTGCCATTGTCTCGCAACTCCACACATGAGATGTACTGG- GTCTCTGG GGCAAAGAGCAACATCATAAAAAGTGTGTCCACCACAAGTCAGCTCCTCCTGGGACGCATGGATGGCCCCAGGA- GGCCAGTG AAATATGAGGAGGATGTGAACCTCGGCTCGGGTACACGAGCTGTGGCAAGCTGTGCTGAGGCTCCTAACATGAA- AATCATCG GCAGGCGCATTGAGAGAATCCGCAATGAACATGCAGAAACATGGTTTCTTGATGAAAACCACCCATACAGGACA- TGGGCCTAC CATGGGAGCTACGAAGCCCCCACGCAAGGATCAGCGTCTTCCCTCGTGAACGGGGTTGTTAGACTCCTGTCAAA- GCCTTGGGA CGTGGTGACTGGAGTTACAGGAATAGCCATGACTGACACCACACCATACGGCCAACAAAGAGTCTTCAAAGAAA- AAGTGGAC ACCAGGGTGCCAGATCCCCAAGAAGGCACTCGCCAGGTAATGAACATAGTCTCTTCCTGGCTGTGGAAGGAGCT- GGGGAAAC GCAAGCGGCCACGCGTCTGCACCAAAGAAGAGTTTATCAACAAGGTGCGCAGCAATGCAGCACTGGGAGCAATA- TTTGAAGA GGAAAAAGAATGGAAGACGGCTGTGGAAGCTGTGAATGATCCAAGGTTTTGGGCCCTAGTGGATAGGGAGAGAG- AACACCA CCTGAGAGGAGAGTGTCACAGCTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAGCAAGGAGAGTTCGGGA- AAGCAA AAGGTAGCCGCGCCATCTGGTACATGTGGTTGGGAGCCAGATTCTTGGAGTTTGAAGCCCTTGGATTCTTGAAC- GAGGACCAT TGGATGGGAAGAGAAAACTCAGGAGGTGGAGTCGAAGGGTTAGGATTGCAAAGACTTGGATACATTCTAGAAGA- AATGAAT CGGGCACCAGGAGGAAAGATGTACGCAGATGACACTGCTGGCTGGGACACCCGCATTAGTAAGTTTGATCTGGA- GAATGAAG CTCTGATTACCAACCAAATGGAGGAAGGGCACAGAACTCTGGCGTTGGCCGTGATTAAATACACATACCAAAAC- AAAGTGGTG AAGGTTCTCAGACCAGCTGAAGGAGGAAAAACAGTTATGGACATCATTTCAAGACAAGACCAGAGAGGGAGTGG- ACAAGTT GTCACTTATGCTCTCAACACATTCACCAACTTGGTGGTGCAGCTTATCCGGAACATGGAAGCTGAGGAAGTGTT- AGAGATGCA AGACTTATGGTTGTTGAGGAAGCCAGAGAAAGTGACCAGATGGTTGCAGAGCAATGGATGGGATAGACTCAAAC- GAATGGC GGTCAGTGGAGATGACTGCGTTGTGAAGCCAATCGATGATAGGTTTGCACATGCCCTCAGGTTCTTGAATGACA- TGGGAAAA GTTAGGAAAGACACACAGGAGTGGAAACCCTCGACTGGATGGAGCAATTGGGAAGAAGTCCCGTTCTGCTCCCA- CCACTTCA ACAAGCTGTACCTCAAGGATGGGAGATCCATTGTGGTCCCTTGCCGCCACCAAGATGAACTGATTGGCCGAGCT- CGCGTCTCA CCAGGGGCAGGATGGAGCATCCGGGAGACTGCCTGTCTTGCAAAATCATATGCGCAGATGTGGCAGCTCCTTTA- TTTCCACAG AAGAGACCTTCGACTGATGGCTAATGCCATTTGCTCGGCTGTGCCAGTTGACTGGGTACCAACTGGGAGAACCA- CCTGGTCAA TCCATGGAAAGGGAGAATGGATGACCACTGAGGACATGCTCATGGTGTGGAATAGAGTGTGGATTGAGGAGAAC- GACCATA TGGAGGACAAGACTCCTGTAACAAAATGGACAGACATTCCCTATCTAGGAAAAAGGGAGGACTTATGGTGTGGA- TCCCTTATA GGGCACAGACCCCGCACCACTTGGGCTGAAAACATCAAAGACACAGTCAACATGGTGCGCAGGATCATAGGTGA- TGAAGAAA AGTACATGGACTATCTATCCACCCAAGTCCGCTACTTGGGTGAGGAAGGGTCCACACCCGGAGTGTTGTAAGCA- CCAATTTTA GTGTTGTCAGGCCTGCTAGTCAGCCACAGTTTGGGGAAAGCTGTGCAGCCTGTAACCCCCCCAGGAGAAGCTGG- GAAACCAA GCTCATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTGAGCCCCTCAGAGGACACTGAG- TCAAAAAA CCCCACGCGCTTGGAAGCGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAAC- TGGAGACT AGCTGTGAATCTCCAGCAGAGGGACTAGTGGTTAGAGGAGACCCCCCGGAAAACGCAAAACAGCATATTGACGC- TGGGAAA GACCAGAGACTCCATGAGTTTCCACCACGCTGGCCGCCAGGCACAGATCGCCGAACAGCGGCGGCCGGTGTGGG- GAAATCCA TGGTTTCT AY632535.2 NC_012532.1 Zika virus strain MR 766, Uganda, complete genome SEQ ID NO: 12 AGTTGTTGATCTGTGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTT- TAATTTGGA TTTGGAAACGAGAGTTTCTGGTCATGAAAAACCCCAAAGAAGAAATCCGGAGGATCCGGATTGTCAATATGCTA- AAACGCGG AGTAGCCCGTGTAAACCCCTTGGGAGGTTTGAAGAGGTTGCCAGCCGGACTTCTGCTGGGTCATGGACCCATCA- GAATGGTTT TGGCGATACTAGCCTTTTTGAGATTTACAGCAATCAAGCCATCACTGGGCCTTATCAACAGATGGGGTTCCGTG- GGGAAAAAA GAGGCTATGGAAATAATAAAGAAGTTCAAGAAAGATCTTGCTGCCATGTTGAGAATAATCAATGCTAGGAAAGA- GAGGAAGA GACGTGGCGCAGACACCAGCATCGGAATCATTGGCCTCCTGCTGACTACAGCCATGGCAGCAGAGATCACTAGA- CGCGGGAG TGCATACTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAGTGAACA- AGTGCCACG TACAGATCATGGACCTCGGGCACATGTGTGACGCCACCATGAGTTATGAGTGCCCTATGCTGGATGAGGGAGTG- GAACCAGA TGATGTCGATTGCTGGTGCAACACGACATCAACTTGGGTTGTGTACGGAACCTGTCATCACAAAAAAGGTGAGG- CACGGCGAT CTAGAAGAGCCGTGACGCTCCCTTCTCACTCTACAAGGAAGTTGCAAACGCGGTCGCAGACCTGGTTAGAATCA- AGAGAATAC ACGAAGCACTTGATCAAGGTTGAAAACTGGATATTCAGGAACCCCGGGTTTGCGCTAGTGGCCGTTGCCATTGC- CTGGCTTTT GGGAAGCTCGACGAGCCAAAAAGTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGTATCAGGT- GCATTGGAG TCAGCAATAGAGACTTCGTGGAGGGCATGTCAGGTGGGACCTGGGTTGATGTTGTCTTGGAACATGGAGGCTGC- GTTACCGT GATGGCACAGGACAAGCCAACAGTCGACATAGAGTTGGTCACGACGACGGTTAGTAACATGGCCGAGGTAAGAT- CCTATTGC TACGAGGCATCGATATCGGACATGGCTTCGGACAGTCGTTGCCCAACACAAGGTGAAGCCTACCTTGACAAGCA- ATCAGACAC TCAATATGTCTGCAAAAGAACATTAGTGGACAGAGGTTGGGGAAACGGTTGTGGACTTTTTGGCAAAGGGAGCT- TGGTGACA TGTGCCAAGTTTACGTGTTCTAAGAAGATGACCGGGAAGAGCATTCAACCGGAAAATCTGGAGTATCGGATAAT- GCTATCAGT GCATGGCTCCCAGCATAGCGGGATGATTGGATATGAAACTGACGAAGATAGAGCGAAAGTCGAGGTTACGCCTA- ATTCACCA AGAGCGGAAGCAACCTTGGGAGGCTTTGGAAGCTTAGGACTTGACTGTGAACCAAGGACAGGCCTTGACTTTTC- AGATCTGTA TTACCTGACCATGAACAATAAGCATTGGTTGGTGCACAAAGAGTGGTTTCATGACATCCCATTGCCTTGGCATG- CTGGGGCAG ACACCGGAACTCCACACTGGAACAACAAAGAGGCATTGGTAGAATTCAAGGATGCCCACGCCAAGAGGCAAACC- GTCGTCGT TCTGGGGAGCCAGGAAGGAGCCGTTCACACGGCTCTCGCTGGAGCTCTAGAGGCTGAGATGGATGGTGCAAAGG- GAAGGCT GTTCTCTGGCCATTTGAAATGCCGCCTAAAAATGGACAAGCTTAGATTGAAGGGCGTGTCATATTCCTTGTGCA- CTGCGGCATT

CACATTCACCAAGGTCCCAGCTGAAACACTGCATGGAACAGTCACAGTGGAGGTGCAGTATGCAGGGACAGATG- GACCCTGC AAGATCCCAGTCCAGATGGCGGTGGACATGCAGACCCTGACCCCAGTTGGAAGGCTGATAACCGCCAACCCCGT- GATTACTGA AAGCACTGAGAACTCAAAGATGATGTTGGAGCTTGACCCACCATTTGGGGATTCTTACATTGTCATAGGAGTTG- GGGACAAGA AAATCACCCACCACTGGCATAGGAGTGGTAGCACCATCGGAAAGGCATTTGAGGCCACTGTGAGAGGCGCCAAG- AGAATGGC AGTCCTGGGGGATACAGCCTGGGACTTCGGATCAGTCGGGGGTGTGTTCAACTCACTGGGTAAGGGCATTCACC- AGATTTTTG GAGCAGCCTTCAAATCACTGTTTGGAGGAATGTCCTGGTTCTCACAGATCCTCATAGGCACGCTGCTAGTGTGG- TTAGGTTTGA ACACAAAGAATGGATCTATCTCCCTCACATGCTTGGCCCTGGGGGGAGTGATGATCTTCCTCTCCACGGCTGTT- TCTGCTGACG TGGGGTGCTCAGTGGACTTCTCAAAAAAGGAAACGAGATGTGGCACGGGGGTATTCATCTATAATGATGTTGAA- GCCTGGAG GGACCGGTACAAGTACCATCCTGACTCCCCCCGCAGATTGGCAGCAGCAGTCAAGCAGGCCTGGGAAGAGGGGA- TCTGTGGG ATCTCATCCGTTTCAAGAATGGAAAACATCATGTGGAAATCAGTAGAAGGGGAGCTCAATGCTATCCTAGAGGA- GAATGGAG TTCAACTGACAGTTGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAAAGATTGCCAGTGCCTGTG- AATGAGCTG CCCCATGGCTGGAAAGCCTGGGGGAAATCGTATTTTGTTAGGGCGGCAAAGACCAACAACAGTTTTGTTGTCGA- CGGTGACAC ACTGAAGGAATGTCCGCTTGAGCACAGAGCATGGAATAGTTTTCTTGTGGAGGATCACGGGTTTGGAGTCTTCC- ACACCAGTG TCTGGCTTAAGGTCAGAGAAGATTACTCATTAGAATGTGACCCAGCCGTCATAGGAACAGCTGTTAAGGGAAGG- GAGGCCGC GCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACACATGGAGGCTGAAGAGGGCCCACCTGATTG- AGATGAAA ACATGTGAATGGCCAAAGTCTCACACATTGTGGACAGATGGAGTAGAAGAAAGTGATCTTATCATACCCAAGTC- TTTAGCTGG TCCACTCAGCCACCACAACACCAGAGAGGGTTACAGAACCCAAGTGAAAGGGCCATGGCACAGTGAAGAGCTTG- AAATCCGG TTTGAGGAATGTCCAGGCACCAAGGTTTACGTGGAGGAGACATGCGGAACTAGAGGACCATCTCTGAGATCAAC- TACTGCAA GTGGAAGGGTCATTGAGGAATGGTGCTGTAGGGAATGCACAATGCCCCCACTATCGTTTCGAGCAAAAGACGGC- TGCTGGTA TGGAATGGAGATAAGGCCCAGGAAAGAACCAGAGAGCAACTTAGTGAGGTCAATGGTGACAGCGGGGTCAACCG- ATCATAT GGACCACTTCTCTCTTGGAGTGCTTGTGATTCTACTCATGGTGCAGGAGGGGTTGAAGAAGAGAATGACCACAA- AGATCATCA TGAGCACATCAATGGCAGTGCTGGTAGTCATGATCTTGGGAGGATTTTCAATGAGTGACCTGGCCAAGCTTGTG- ATCCTGATG GGTGCTACTTTCGCAGAAATGAACACTGGAGGAGATGTAGCTCACTTGGCATTGGTAGCGGCATTTAAAGTCAG- ACCAGCCTT GCTGGTCTCCTTCATTTTCAGAGCCAATTGGACACCCCGTGAGAGCATGCTGCTAGCCCTGGCTTCGTGTCTTC- TGCAAACTGC GATCTCTGCTCTTGAAGGTGACTTGATGGTCCTCATTAATGGATTTGCTTTGGCCTGGTTGGCAATTCGAGCAA- TGGCCGTGCC ACGCACTGACAACATCGCTCTACCAATCTTGGCTGCTCTAACACCACTAGCTCGAGGCACACTGCTCGTGGCAT- GGAGAGCGG GCCTGGCTACTTGTGGAGGGATCATGCTCCTCTCCCTGAAAGGGAAAGGTAGTGTGAAGAAGAACCTGCCATTT- GTCATGGCC CTGGGATTGACAGCTGTGAGGGTAGTAGACCCTATTAATGTGGTAGGACTACTGTTACTCACAAGGAGTGGGAA- GCGGAGCT GGCCCCCTAGTGAAGTTCTCACAGCCGTTGGCCTGATATGTGCACTGGCCGGAGGGTTTGCCAAGGCAGACATT- GAGATGGCT GGACCCATGGCTGCAGTAGGCTTGCTAATTGTCAGCTATGTGGTCTCGGGAAAGAGTGTGGACATGTACATTGA- AAGAGCAG GTGACATCACATGGGAAAAGGACGCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGAGT- GGTGACTT CTCCTTGGTAGAGGAAGATGGTCCACCCATGAGAGAGATCATACTCAAGGTGGTCCTGATGGCCATCTGTGGCA- TGAACCCAA TAGCTATACCTTTTGCTGCAGGAGCGTGGTATGTGTATGTGAAGACTGGGAAAAGGAGTGGCGCCCTCTGGGAC- GTGCCTGC TCCCAAAGAAGTGAAGAAAGGAGAGACCACAGATGGAGTGTACAGAGTGATGACTCGCAGACTGCTAGGTTCAA- CACAGGTT GGAGTGGGAGTCATGCAAGAGGGAGTCTTCCACACCATGTGGCACGTTACAAAAGGAGCCGCACTGAGGAGCGG- TGAGGGA AGACTTGATCCATACTGGGGGGATGTCAAGCAGGACTTGGTGTCATACTGTGGGCCTTGGAAGTTGGATGCAGC- TTGGGATG GACTCAGCGAGGTACAGCTTTTGGCCGTACCTCCCGGAGAGAGGGCCAGAAACATTCAGACCCTGCCTGGAATA- TTCAAGACA AAGGACGGGGACATCGGAGCAGTTGCTCTGGACTACCCTGCAGGGACCTCAGGATCTCCGATCCTAGACAAATG- TGGAAGAG TGATAGGACTCTATGGCAATGGGGTTGTGATCAAGAATGGAAGCTATGTTAGTGCTATAACCCAGGGAAAGAGG- GAGGAGG AGACTCCGGTTGAATGTTTCGAACCCTCGATGCTGAAGAAGAAGCAGCTAACTGTCTTGGATCTGCATCCAGGA- GCCGGAAAA ACCAGGAGAGTTCTTCCTGAAATAGTCCGTGAAGCCATAAAAAAGAGACTCCGGACAGTGATCTTGGCACCAAC- TAGGGTTGT CGCTGCTGAGATGGAGGAGGCCTTGAGAGGACTTCCGGTGCGTTACATGACAACAGCAGTCAACGTCACCCATT- CTGGGACA GAAATCGTTGATTTGATGTGCCATGCCACTTTCACTTCACGCTTACTACAACCCATCAGAGTCCCTAATTACAA- TCTCAACATCAT GGATGAAGCCCACTTCACAGACCCCTCAAGTATAGCTGCAAGAGGATACATATCAACAAGGGTTGAAATGGGCG- AGGCGGCT GCCATTTTTATGACTGCCACACCACCAGGAACCCGTGATGCGTTTCCTGACTCTAACTCACCAATCATGGACAC- AGAAGTGGAA GTCCCAGAGAGAGCCTGGAGCTCAGGCTTTGATTGGGTGACAGACCATTCTGGGAAAACAGTTTGGTTCGTTCC- AAGCGTGA GAAACGGAAATGAAATCGCAGCCTGTCTGACAAAGGCTGGAAAGCGGGTCATACAGCTCAGCAGGAAGACTTTT- GAGACAGA ATTTCAGAAAACAAAAAATCAAGAGTGGGACTTTGTCATAACAACTGACATCTCAGAGATGGGCGCCAACTTCA- AGGCTGACC GGGTCATAGACTCTAGGAGATGCCTAAAACCAGTCATACTTGATGGTGAGAGAGTCATCTTGGCTGGGCCCATG- CCTGTCACG CATGCTAGTGCTGCTCAGAGGAGAGGACGTATAGGCAGGAACCCTAACAAACCTGGAGATGAGTACATGTATGG- AGGTGGG TGTGCAGAGACTGATGAAGGCCATGCACACTGGCTTGAAGCAAGAATGCTTCTTGACAACATCTACCTCCAGGA- TGGCCTCAT AGCCTCGCTCTATCGGCCTGAGGCCGATAAGGTAGCCGCCATTGAGGGAGAGTTTAAGCTGAGGACAGAGCAAA- GGAAGAC CTTCGTGGAACTCATGAAGAGAGGAGACCTTCCCGTCTGGCTAGCCTATCAGGTTGCATCTGCCGGAATAACTT- ACACAGACA GAAGATGGTGCTTTGATGGCACAACCAACAACACCATAATGGAAGACAGTGTACCAGCAGAGGTTTGGACAAAG- TATGGAGA GAAGAGAGTGCTCAAACCGAGATGGATGGATGCTAGGGTCTGTTCAGACCATGCGGCCCTGAAGTCGTTCAAAG- AATTCGCC GCTGGAAAAAGAGGAGCGGCTTTGGGAGTAATGGAGGCCCTGGGAACACTGCCAGGACACATGACAGAGAGGTT- TCAGGAA GCCATTGACAACCTCGCCGTGCTCATGCGAGCAGAGACTGGAAGCAGGCCTTATAAGGCAGCGGCAGCCCAACT- GCCGGAGA CCCTAGAGACCATTATGCTCTTAGGTTTGCTGGGAACAGTTTCACTGGGGATCTTCTTCGTCTTGATGCGGAAT- AAGGGCATCG GGAAGATGGGCTTTGGAATGGTAACCCTTGGGGCCAGTGCATGGCTCATGTGGCTTTCGGAAATTGAACCAGCC- AGAATTGC ATGTGTCCTCATTGTTGTGTTTTTATTACTGGTGGTGCTCATACCCGAGCCAGAGAAGCAAAGATCTCCCCAAG- ATAACCAGAT GGCAATTATCATCATGGTGGCAGTGGGCCTTCTAGGTTTGATAACTGCAAACGAACTTGGATGGCTGGAAAGAA- CAAAAAAT GACATAGCTCATCTAATGGGAAGGAGAGAAGAAGGAGCAACCATGGGATTCTCAATGGACATTGATCTGCGGCC- AGCCTCCG CCTGGGCTATCTATGCCGCATTGACAACTCTCATCACCCCAGCTGTCCAACATGCGGTAACCACTTCATACAAC- AACTACTCCTT AATGGCGATGGCCACACAAGCTGGAGTGCTGTTTGGCATGGGCAAAGGGATGCCATTTATGCATGGGGACCTTG- GAGTCCCG CTGCTAATGATGGGTTGCTATTCACAATTAACACCCCTGACTCTGATAGTAGCTATCATTCTGCTTGTGGCGCA- CTACATGTACT TGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCCAGAAAAGGACAGCAGCTGGCATCATGAAGAATCCC- GTTGTGGA TGGAATAGTGGTAACTGACATTGACACAATGACAATAGACCCCCAGGTGGAGAAGAAGATGGGACAAGTGTTAC- TCATAGCA GTAGCCATCTCCAGTGCTGTGCTGCTGCGGACCGCCTGGGGATGGGGGGAGGCTGGAGCTCTGATCACAGCAGC- GACCTCCA CCTTGTGGGAAGGCTCTCCAAACAAATACTGGAACTCCTCTACAGCCACCTCACTGTGCAACATCTTCAGAGGA- AGCTATCTGG CAGGAGCTTCCCTTATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTGGGACGGGAGAG- ACTCTGG GAGAGAAGTGGAAAGCTCGTCTGAATCAGATGTCGGCCCTGGAGTTCTACTCTTATAAAAAGTCAGGTATCACT- GAAGTGTGT AGAGAGGAGGCTCGCCGTGCCCTCAAGGATGGAGTGGCCACAGGAGGACATGCCGTATCCCGGGGAAGTGCAAA- GATCAGA TGGTTGGAGGAGAGAGGATATCTGCAGCCCTATGGGAAGGTTGTTGACCTCGGATGTGGCAGAGGGGGCTGGAG- CTATTAT GCCGCCACCATCCGCAAAGTGCAGGAGGTGAGAGGATACACAAAGGGAGGTCCCGGTCATGAAGAACCCATGCT- GGTGCAA AGCTATGGGTGGAACATAGTTCGTCTCAAGAGTGGAGTGGACGTCTTCCACATGGCGGCTGAGCCGTGTGACAC- TCTGCTGTG TGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAGACACGAACACTCAGAGTGCTCTCTATGGTGGGGG- ACTGGCTT GAAAAAAGACCAGGGGCCTTCTGTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAACCATGGAGCG- ACTGCAAC GTAGGCATGGGGGAGGATTAGTCAGAGTGCCATTGTGTCGCAACTCCACACATGAGATGTACTGGGTCTCTGGG- GCAAAGAG CAACATCATAAAAAGTGTGTCCACCACAAGTCAGCTCCTCCTGGGACGCATGGATGGCCCCAGGAGGCCAGTGA- AATATGAG GAGGATGTGAACCTCGGCTCGGGTACACGAGCTGTGGCAAGCTGTGCTGAGGCTCCTAACATGAAAATCATCGG- CAGGCGCA TTGAGAGAATCCGCAATGAACATGCAGAAACATGGTTTCTTGATGAAAACCACCCATACAGGACATGGGCCTAC- CATGGGAGC TACGAAGCCCCCACGCAAGGATCAGCGTCTTCCCTCGTGAACGGGGTTGTTAGACTCCTGTCAAAGCCTTGGGA- CGTGGTGAC TGGAGTTACAGGAATAGCCATGACTGACACCACACCATACGGCCAACAAAGAGTCTTCAAAGAAAAAGTGGACA- CCAGGGTG CCAGATCCCCAAGAAGGCACTCGCCAGGTAATGAACATAGTCTCTTCCTGGCTGTGGAAGGAGCTGGGGAAACG-

CAAGCGGC CACGCGTCTGCACCAAAGAAGAGTTTATCAACAAGGTGCGCAGCAATGCAGCACTGGGAGCAATATTTGAAGAG- GAAAAAGA ATGGAAGACGGCTGTGGAAGCTGTGAATGATCCAAGGTTTTGGGCCCTAGTGGATAGGGAGAGAGAACACCACC- TGAGAGG AGAGTGTCACAGCTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAGCAAGGAGAGTTCGGGAAAGCAAAAG- GTAGCC GCGCCATCTGGTACATGTGGTTGGGAGCCAGATTCTTGGAGTTTGAAGCCCTTGGATTCTTGAACGAGGACCAT- TGGATGGGA AGAGAAAACTCAGGAGGTGGAGTCGAAGGGTTAGGATTGCAAAGACTTGGATACATTCTAGAAGAAATGAATCG- GGCACCA GGAGGAAAGATGTACGCAGATGACACTGCTGGCTGGGACACCCGCATTAGTAAGTTTGATCTGGAGAATGAAGC- TCTGATTA CCAACCAAATGGAGGAAGGGCACAGAACTCTGGCGTTGGCCGTGATTAAATACACATACCAAAACAAAGTGGTG- AAGGTTCT CAGACCAGCTGAAGGAGGAAAAACAGTTATGGACATCATTTCAAGACAAGACCAGAGAGGGAGTGGACAAGTTG- TCACTTAT GCTCTCAACACATTCACCAACTTGGTGGTGCAGCTTATCCGGAACATGGAAGCTGAGGAAGTGTTAGAGATGCA- AGACTTATG GTTGTTGAGGAAGCCAGAGAAAGTGACCAGATGGTTGCAGAGCAATGGATGGGATAGACTCAAACGAATGGCGG- TCAGTGG AGATGACTGCGTTGTGAAGCCAATCGATGATAGGTTTGCACATGCCCTCAGGTTCTTGAATGACATGGGAAAAG- TTAGGAAAG ACACACAGGAGTGGAAACCCTCGACTGGATGGAGCAATTGGGAAGAAGTCCCGTTCTGCTCCCACCACTTCAAC- AAGCTGTAC CTCAAGGATGGGAGATCCATTGTGGTCCCTTGCCGCCACCAAGATGAACTGATTGGCCGAGCTCGCGTCTCACC- AGGGGCAG GATGGAGCATCCGGGAGACTGCCTGTCTTGCAAAATCATATGCGCAGATGTGGCAGCTCCTTTATTTCCACAGA- AGAGACCTT CGACTGATGGCTAATGCCATTTGCTCGGCTGTGCCAGTTGACTGGGTACCAACTGGGAGAACCACCTGGTCAAT- CCATGGAAA GGGAGAATGGATGACCACTGAGGACATGCTCATGGTGTGGAATAGAGTGTGGATTGAGGAGAACGACCATATGG- AGGACAA GACTCCTGTAACAAAATGGACAGACATTCCCTATCTAGGAAAAAGGGAGGACTTATGGTGTGGATCCCTTATAG- GGCACAGAC CCCGCACCACTTGGGCTGAAAACATCAAAGACACAGTCAACATGGTGCGCAGGATCATAGGTGATGAAGAAAAG- TACATGGA CTATCTATCCACCCAAGTCCGCTACTTGGGTGAGGAAGGGTCCACACCCGGAGTGTTGTAAGCACCAATTTTAG- TGTTGTCAGG CCTGCTAGTCAGCCACAGTTTGGGGAAAGCTGTGCAGCCTGTAACCCCCCCAGGAGAAGCTGGGAAACCAAGCT- CATAGTCA GGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTGAGCCCCTCAGAGGACACTGAGTCAAAAAACCC- CACGCGCT TGGAAGCGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAACTGGAGACTAGC- TGTGAATC TCCAGCAGAGGGACTAGTGGTTAGAGGAGACCCCCCGGAAAACGCAAAACAGCATATTGACGTGGGAAAGACCA- GAGACTC CATGAGTTTCCACCACGCTGGCCGCCAGGCACAGATCGCCGAACTTCGGCGGCCGGTGTGGGGAAATCCATGGT- TTCT KJ776791.1, Zika virus strain H/PF/2013 polyprotein gene, complete cds SEQ ID NO: 13 AGTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCATGAAAAACCCAAAAAAGAAATCCGGA- GGATTCCGG ATTGTCAATATGCTAAAACGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACT- TCTGCTGG GTCATGGGCCCATCAGGATGGTCTTGGCGATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGT- CTCATCAATA GATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAATAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGCTG- AGAATAA TCAATGCTAGGAAGGAGAAGAAGAGACGAGGCGCAGATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACA- GCTATGGC AGCGGAGGTCACTAGACGTGGGAGTGCATACTATATGTACTTGGACAGAAACGACGCTGGGGAGGCCATATCTT- TTCCAACC ACATTGGGGATGAATAAGTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGA- ATGCCCTAT GCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACACGACGTCAACTTGGGTTGTGTACGGAA- CCTGCCAT CACAAAAAAGGTGAAGCACGGAGATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACTAGGAAGCTGCAAAC- GCGGTCGC AAACCTGGTTGGAATCAAGAGAATACACAAAGCACTTGATTAGAGTCGAAAATTGGATATTCAGGAACCCTGGC- TTCGCGTTA GCAGCAGCTGCCATCGCTTGGCTTTTGGGAAGCTCAACGAGCCAAAAAGTCATATACTTGGTCATGATACTGCT- GATTGCCCCG GCATACAGCATCAGGTGCATAGGAGTCAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTGA- TGTTGTCT TGGAACATGGAGGTTGTGTCACCGTAATGGCACAGGACAAACCGACTGTCGACATAGAGCTGGTTACAACAACA- GTCAGCAA CATGGCGGAGGTAAGATCCTACTGCTATGAGGCATCAATATCGGACATGGCTTCGGACAGCCGCTGCCCAACAC- AAGGTGAA GCCTACCTTGACAAGCAATCAGACACTCAATATGTCTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGG- ATGTGGAC TTTTTGGCAAAGGGAGCCTGGTGACATGCGCTAAGTTTGCATGCTCCAAGAAAATGACCGGGAAGAGCATCCAG- CCAGAGAA TCTGGAGTACCGGATAATGCTGTCAGTTCATGGCTCCCAGCACAGTGGGATGATCGTTAATGACACAGGACATG- AAACTGATG AGAATAGAGCGAAGGTTGAGATAACGCCCAATTCACCAAGAGCCGAAGCCACCCTGGGGGGTTTTGGAAGCCTA- GGACTTGA TTGTGAACCGAGGACAGGCCTTGACTTTTCAGATTTGTATTACTTGACTATGAATAACAAGCACTGGTTGGTTC- ACAAGGAGTG GTTCCACGACATTCCATTACCTTGGCACGCTGGGGCAGACACCGGAACTCCACACTGGAACAACAAAGAAGCAC- TGGTAGAGT TCAAGGACGCACATGCCAAAAGGCAAACTGTCGTGGTTCTAGGGAGTCAAGAAGGAGCAGTTCACACGGCCCTT- GCTGGAGC TCTGGAGGCTGAGATGGATGGTGCAAAGGGAAGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATA- AACTTAGA TTGAAGGGCGTGTCATACTCCTTGTGTACCGCAGCGTTCACATTCACCAAGATCCCGGCTGAAACACTGCACGG- GACAGTCAC AGTGGAGGTACAGTACGCAGGGACAGATGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGACATGCAAACTC- TGACCCCA GTTGGGAGGTTGATAACCGCTAACCCCGTAATCACTGAAAGCACTGAGAACTCTAAGATGATGCTGGAACTTGA- TCCACCATT TGGGGACTCTTACATTGTCATAGGAGTCGGGGAGAAGAAGATCACCCACCACTGGCACAGGAGTGGCAGCACCA- TTGGAAAA GCATTTGAAGCCACTGTGAGAGGTGCCAAGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGT- TGGAGGCG CTCTCAACTCATTGGGCAAGGGCATCCATCAAATTTTTGGAGCAGCTTTCAAATCATTGTTTGGAGGAATGTCC- TGGTTCTCACA AATTCTCATTGGAACGTTGCTGATGTGGTTGGGTCTGAACACAAAGAATGGATCTATTTCCCTTATGTGCTTGG- CCTTAGGGGG AGTGTTGATCTTCTTATCCACAGCTGTCTCTGCTGATGTGGGGTGCTCGGTGGACTTCTCAAAGAAGGAGACGA- GATGCGGTA CAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTACAAGTACCATCCTGACTCCCCCCGTAGA- TTGGCAGCA GCAGTCAAGCAAGCCTGGGAAGATGGTATCTGTGGGATCTCCTCTGTTTCAAGAATGGAAAACATCATGTGGAG- ATCAGTAG AAGGGGAGCTCAACGCAATCCTGGAAGAGAATGGAGTTCAACTGACGGTCGTTGTGGGATCTGTAAAAAACCCC- ATGTGGAG AGGTCCACAGAGATTGCCCGTGCCTGTGAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCGTACTTCG- TCAGAGCA GCAAAGACAAATAACAGCTTTGTCGTGGATGGTGACACACTGAAGGAATGCCCACTCAAACATAGAGCATGGAA- CAGCTTTCT TGTGGAGGATCATGGGTTCGGGGTATTTCACACTAGTGTCTGGCTCAAGGTTAGAGAAGATTATTCATTAGAGT- GTGATCCAG CCGTTATTGGAACAGCTGTTAAGGGAAAGGAGGCTGTACACAGTGATCTAGGCTACTGGATTGAGAGTGAGAAG- AATGACAC ATGGAGGCTGAAGAGGGCCCATCTGATCGAGATGAAAACATGTGAATGGCCAAAGTCCCACACATTGTGGACAG- ATGGAATA GAAGAGAGTGATCTGATCATACCCAAGTCTTTAGCTGGGCCACTCAGCCATCACAATACCAGAGAGGGCTACAG- GACCCAAAT GAAAGGGCCATGGCACAGTGAAGAGCTTGAAATTCGGTTTGAGGAATGCCCAGGCACTAAGGTCCACGTGGAGG- AAACATG TGGAACAAGAGGACCATCTCTGAGATCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGTGCTGCAGGGAGT- GCACAAT GCCCCCACTGTCGTTCCGGGCTAAAGATGGCTGTTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAA- GTAACTTA GTAAGGTCAATGGTGACTGCAGGATCAACTGATCACATGGATCACTTCTCCCTTGGAGTGCTTGTGATTCTGCT- CATGGTGCAG GAAGGGCTGAAGAAGAGAATGACCACAAAGATCATCATAAGCACATCGATGGCAGTGCTGGTAGCTATGATCCT- GGGAGGAT TTTCAATGAGTGACCTGGCTAAGCTTGCAATTTTGATGGGTGCCACCTTCGCGGAAATGAACACTGGAGGAGAT- GTAGCTCAT CTGGCGCTGATAGCGGCATTCAAAGTCAGACCAGCGTTGCTGGTATCTTTCATCTTCAGAGCTAATTGGACACC- CCGTGAAAGC ATGCTGCTGGCCTTGGCCTCGTGTCTTTTGCAAACTGCGATCTCCGCCTTGGAAGGCGACCTGATGGTTCTCAT- CAATGGTTTT GCTTTGGCCTGGTTGGCAATACGAGCGATGGTTGTTCCACGCACTGATAACATCACCTTGGCAATCCTGGCTGC- TCTGACACCA CTGGCCCGGGGCACACTGCTTGTGGCGTGGAGAGCAGGCCTTGCTACTTGCGGGGGGTTTATGCTCCTCTCTCT- GAAGGGAA AAGGCAGTGTGAAGAAGAACTTACCATTTGTCATGGCCCTGGGACTAACCGCTGTGAGGCTGGTCGACCCCATC- AACGTGGTG GGACTGCTGTTGCTCACAAGGAGTGGGAAGCGGAGCTGGCCCCCTAGCGAAGTACTCACAGCTGTTGGCCTGAT- ATGCGCAT TGGCTGGAGGGTTCGCCAAGGCAGATATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAGT- TACGTGGT CTCAGGAAAGAGTGTGGACATGTACATTGAAAGAGCAGGTGACATCACATGGGAAAAAGATGCGGAAGTCACTG- GAAACAG TCCCCGGCTCGATGTGGCGCTAGATGAGAGTGGTGATTTCTCCCTGGTGGAGGATGACGGTCCCCCCATGAGAG- AGATCATAC TCAAGGTGGTCCTGATGACCATCTGTGGCATGAACCCAATAGCCATACCCTTTGCAGCTGGAGCGTGGTACGTA- TACGTGAAG ACTGGAAAAAGGAGTGGTGCTCTATGGGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCACAGATGG- AGTGTAC AGAGTAATGACTCGTAGACTGCTAGGTTCAACACAAGTTGGAGTGGGAGTTATGCAAGAGGGGGTCTTTCACAC- TATGTGGC ACGTCACAAAAGGATCCGCGCTGAGAAGCGGTGAAGGGAGACTTGATCCATACTGGGGAGATGTCAAGCAGGAT- CTGGTGT CATACTGTGGTCCATGGAAGCTAGATGCCGCCTGGGACGGGCACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCC-

GGAGAGAG AGCGAGGAACATCCAGACTCTGCCCGGAATATTTAAGACAAAGGATGGGGACATTGGAGCGGTTGCGCTGGATT- ACCCAGCA GGAACTTCAGGATCTCCAATCCTAGACAAGTGTGGGAGAGTGATAGGACTTTATGGCAATGGGGTCGTGATCAA- AAATGGGA GTTATGTTAGTGCCATCACCCAAGGGAGGAGGGAGGAAGAGACTCCTGTTGAGTGCTTCGAGCCTTCGATGCTG- AAGAAGAA GCAGCTAACTGTCTTAGACTTGCATCCTGGAGCTGGGAAAACCAGGAGAGTTCTTCCTGAAATAGTCCGTGAAG- CCATAAAAA CAAGACTCCGTACTGTGATCTTAGCTCCAACCAGGGTTGTCGCTGCTGAAATGGAGGAAGCCCTTAGAGGGCTT- CCAGTGCGT TATATGACAACAGCAGTCAATGTCACCCACTCTGGAACAGAAATCGTCGACTTAATGTGCCATGCCACCTTCAC- TTCACGTCTAC TACAGCCAATCAGAGTCCCCAACTATAATCTGTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATA- GCAGCAAGAG GATACATTTCAACAAGGGTTGAGATGGGCGAGGCGGCTGCCATCTTCATGACCGCCACGCCACCAGGAACCCGT- GACGCATTT CCGGACTCCAACTCACCAATTATGGACACCGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTCAGGCTTTGATTG- GGTGACGG ATCATTCTGGAAAAACAGTTTGGTTTGTTCCAAGCGTGAGGAACGGCAATGAGATCGCAGCTTGTCTGACAAAG- GCTGGAAAA CGGGTCATACAGCTCAGCAGAAAGACTTTTGAGACAGAGTTCCAGAAAACAAAACATCAAGAGTGGGACTTTGT- CGTGACAA CTGACATTTCAGAGATGGGCGCCAACTTTAAAGCTGACCGTGTCATAGATTCCAGGAGATGCCTAAAGCCGGTC- ATACTTGAT GGCGAGAGAGTCATTCTGGCTGGACCCATGCCTGTCACACATGCCAGCGCTGCCCAGAGGAGGGGGCGCATAGG- CAGGAAT CCCAACAAACCTGGAGATGAGTATCTGTATGGAGGTGGGTGCGCAGAGACTGACGAAGACCATGCACACTGGCT- TGAAGCAA GAATGCTCCTTGACAATATTTACCTCCAAGATGGCCTCATAGCCTCGCTCTATCGACCTGAGGCCGACAAAGTA- GCAGCCATTG AGGGAGAGTTCAAGCTTAGGACGGAGCAAAGGAAGACCTTTGTGGAACTCATGAAAAGAGGAGATCTTCCTGTT- TGGCTGGC CTATCAGGTTGCATCTGCCGGAATAACCTACACAGATAGAAGATGGTGCTTTGATGGCACGACCAACAACACCA- TAATGGAAG ACAGTGTGCCGGCAGAGGTGTGGACCAGACACGGAGAGAAAAGAGTGCTCAAACCGAGGTGGATGGACGCCAGA- GTTTGTT CAGATCATGCGGCCCTGAAGTCATTCAAGGAGTTTGCCGCTGGGAAAAGAGGAGCGGCTTTTGGAGTGATGGAA- GCCCTGGG AACACTGCCAGGACACATGACAGAGAGATTCCAGGAAGCCATTGACAACCTCGCTGTGCTCATGCGGGCAGAGA- CTGGAAGC AGGCCTTACAAAGCCGCGGCGGCCCAATTGCCGGAGACCCTAGAGACCATTATGCTTTTGGGGTTGCTGGGAAC- AGTCTCGCT GGGAATCTTTTTCGTCTTGATGAGGAACAAGGGCATAGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCA- GCGCATGG CTCATGTGGCTCTCGGAAATTGAGCCAGCCAGAATTGCATGTGTCCTCATTGTTGTGTTCCTATTGCTGGTGGT- GCTCATACCTG AGCCAGAAAAGCAAAGATCTCCCCAGGACAACCAAATGGCAATCATCATCATGGTAGCAGTAGGTCTTCTGGGC- TTGATTACC GCCAATGAACTCGGATGGTTGGAGAGAACAAAGAGTGACCTAAGCCATCTAATGGGAAGGAGAGAGGAGGGGGC- AACCATA GGATTCTCAATGGACATTGACCTGCGGCCAGCCTCAGCTTGGGCCATCTATGCTGCCTTGACAACTTTCATTAC- CCCAGCCGTCC AACATGCAGTGACCACTTCATACAACAACTACTCCTTAATGGCGATGGCCACGCAAGCTGGAGTGTTGTTTGGT- ATGGGCAAA GGGATGCCATTCTACGCATGGGACTTTGGAGTCCCGCTGCTAATGATAGGTTGCTACTCACAATTAACACCCCT- GACCCTAATA GTGGCCATCATTTTGCTCGTGGCGCACTACATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCGTGCTGC- CCAGAAGAG AACGGCAGCTGGCATCATGAAGAACCCTGTTGTGGATGGAATAGTGGTGACTGACATTGACACAATGACAATTG- ACCCCCAA GTGGAGAAAAAGATGGGACAGGTGCTACTCATAGCAGTAGCCGTCTCCAGCGCCATACTGTCGCGGACCGCCTG- GGGGTGG GGGGAGGCTGGGGCCCTGATCACAGCGGCAACTTCCACTTTGTGGGAAGGCTCTCCGAACAAGTACTGGAACTC- CTCTACAGC CACTTCACTGTGTAACATTTTTAGGGGAAGTTACTTGGCTGGAGCTTCTCTAATCTACACAGTAACAAGAAACG- CTGGCTTGGT CAAGAGACGTGGGGGTGGAACAGGAGAGACCCTGGGAGAGAAATGGAAGGCCCGCTTGAACCAGATGTCGGCCC- TGGAGT TCTACTCCTACAAAAAGTCAGGCATCACCGAGGTGTGCAGAGAAGAGGCCCGCCGCGCCCTCAAGGACGGTGTG- GCAACGGG AGGCCATGCTGTGTCCCGAGGAAGTGCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCTGCAGCCCTATGGAA- AGGTCATT GATCTTGGATGTGGCAGAGGGGGCTGGAGTTACTACGCCGCCACCATCCGCAAAGTTCAAGAAGTGAAAGGATA- CACAAAAG GAGGCCCTGGTCATGAAGAACCCATGTTGGTGCAAAGCTATGGGTGGAACATAGTCCGTCTTAAGAGTGGGGTG- GACGTCTT TCATATGGCGGCTGAGCCGTGTGACACGTTGCTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAG- AAGCACGG ACGCTCAGAGTCCTCTCCATGGTGGGGGATTGGCTTGAAAAAAGACCAGGAGCCTTTTGTATAAAAGTGTTGTG- CCCATACAC CAGCACTATGATGGAAACCCTGGAGCGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGAGTGCCACTCTCCC- GCAACTCT ACACATGAGATGTACTGGGTCTCTGGAGCGAAAAGCAACACCATAAAAAGTGTGTCCACCACGAGCCAGCTCCT- CTTGGGGC GCATGGACGGGCCCAGGAGGCCAGTGAAATATGAGGAGGATGTGAATCTCGGCTCTGGCACGCGGGCTGTGGTA- AGCTGCG CTGAAGCTCCCAACATGAAGATCATTGGTAACCGCATTGAAAGGATCCGCAGTGAGCACGCGGAAACGTGGTTC- TTTGACGA GAACCACCCATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGTCAGCGTCCTCTCTAA- TAAACGGGG TTGTCAGGCTCCTGTCAAAACCCTGGGATGTGGTGACTGGAGTCACAGGAATAGCCATGACCGACACCACACCG- TATGGTCAG CAAAGAGTTTTCAAGGAAAAAGTGGACACTAGGGTGCCAGACCCCCAAGAAGGCACTCGTCAGGTTATGAGCAT- GGTCTCTTC CTGGTTGTGGAAAGAGCTAGGCAAACACAAACGGCCACGAGTCTGTACCAAAGAAGAGTTCATCAACAAGGTTC- GTAGCAAT GCAGCATTAGGGGCAATATTTGAAGAGGAAAAAGAGTGGAAGACTGCAGTGGAAGCTGTGAACGATCCAAGGTT- CTGGGCT CTAGTGGACAAGGAAAGAGAGCACCACCTGAGAGGAGAGTGCCAGAGTTGTGTGTACAACATGATGGGAAAAAG- AGAAAAG AAACAAGGGGAATTTGGAAAGGCCAAGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAGATTTCTAGA- GTTCGAAG CCCTTGGATTCTTGAACGAGGATCACTGGATGGGGAGAGAGAACTCAGGAGGTGGTGTTGAAGGGCTGGGATTA- CAAAGAC TCGGATATGTCCTAGAAGAGATGAGTCGCATACCAGGAGGAAGGATGTATGCAGATGACACTGCTGGCTGGGAC- ACCCGCAT CAGCAGGTTTGATCTGGAGAATGAAGCTCTAATCACCAACCAAATGGAGAAAGGGCACAGGGCCTTGGCATTGG- CCATAATC AAGTACACATACCAAAACAAAGTGGTAAAGGTCCTTAGACCAGCTGAAAAAGGGAAGACAGTTATGGACATTAT- TTCGAGAC AAGACCAAAGGGGGAGCGGACAAGTTGTCACTTACGCTCTTAACACATTTACCAACCTAGTGGTGCAACTCATT- CGGAATATG GAGGCTGAGGAAGTTCTAGAGATGCAAGACTTGTGGCTGCTGCGGAGGTCAGAGAAAGTGACCAACTGGTTGCA- GAGCAAC GGATGGGATAGGCTCAAACGAATGGCAGTCAGTGGAGATGATTGCGTTGTGAAGCCAATTGATGATAGGTTTGC- ACATGCCC TCAGGTTCTTGAATGATATGGGAAAAGTTAGGAAGGACACACAAGAGTGGAAACCCTCAACTGGATGGGACAAC- TGGGAAG AAGTTCCGTTTTGCTCCCACCACTTCAACAAGCTCCATCTCAAGGACGGGAGGTCCATTGTGGTTCCCTGCCGC- CACCAAGATG AACTGATTGGCCGGGCCCGCGTCTCTCCAGGGGCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCA- TATGCGCA AATGTGGCAGCTCCTTTATTTCCACAGAAGGGACCTCCGACTGATGGCCAATGCCATTTGTTCATCTGTGCCAG- TTGACTGGGT TCCAACTGGGAGAACTACCTGGTCAATCCATGGAAAGGGAGAATGGATGACCACTGAAGACATGCTTGTGGTGT- GGAACAGA GTGTGGATTGAGGAGAACGACCACATGGAAGACAAGACCCCAGTTACGAAATGGACAGACATTCCCTATTTGGG- AAAAAGGG AAGACTTGTGGTGTGGATCTCTCATAGGGCACAGACCGCGCACCACCTGGGCTGAGAACATTAAAAACACAGTC- AACATGGT GCGCAGGATCATAGGTGATGAAGAAAAGTACATGGACTACCTATCCACCCAAGTTCGCTACTTGGGTGAAGAAG- GGTCTACA CCTGGAGTGCTGTAAGCACCAATCTTAGTGTTGTCAGGCCTGCTAGTCAGCCACAGCTTGGGGAAAGCTGTGCA- GCCTGTGAC CCCCCCAGGAGAAGCTGGGAAACCAAGCCTATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGC- CTGTGAGC CCCTCAGAGGACACTGAGTCAAAAAACCCCACGCGCTTGGAGGCGCAGGATGGGAAAAGAAGGTGGCGACCTTC- CCCACCCT TCAATCTGGGGCCTGAACTGGAGATCAGCTGTGGATCTCCAGAAGAGGGACTAGTGGTTAGAGGAG

In some embodiments, the Zika virus has a RNA genome corresponding to the DNA sequence provided by the nucleic acid sequence of any one of SEQ ID NOs: 2-13 or 73. In some embodiments, the Zika virus has a variant genome that is at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%. 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% identical to any one of SEQ ID NOs: 2-13 or 78.

Provided below are amino acid sequences of the E-proteins of Zika strains that may be used in the methods, compositions, and/or vaccines described herein.

TABLE-US-00006 isol-ARB15076.AHF49784.1.Central_African_Republic/291-788 Flavivirus envelope glycoprotein E. SEQ ID NO: 14 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDENRA- KVEVTPNSPRA EATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHA- KRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTVEVQYAG- TDGPCKVPAQM AVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAK- RMAVLGDTAWDF GSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFLSTAVSA isol-IbH_30656.AEN75265.1.Nigeria/291-788 Flavivirus envelope glycoprotein E. SEQ ID NO: 15 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDENRA- KVEVTPNSPRA EATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHSGADTETPHWNNKEALVEFKDAHA- KRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTVEVQYAG- RDGPCKVPAQM AVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSIIGKAFEATVRGAK- RMAVLGDTAWDF GSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFLSTAVSA ArB1362.AHL43500.1.-/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 16 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDXXXX- XXXNRAEVEVT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLG DTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFLS- TAVSA ArD128000.AHL43502.1.-/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 17 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMXXXXXGHE- TDENRAKVEVT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHRLVRKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWLKKGSSIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGVFNSLGKGVHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFLST- AVSA ArD158095.AHL43505.1.-/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 18 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDIGHE- TDENRAKVEVT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLG DTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFLS- TAVSA ArD158084.AHL43504.1.-/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 19 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDIGHE- TDENRAKVEVT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLG DTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFLS- TAVSA isol-ARB13565.AHF49783.1.Central_African_Republic/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 20 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDIGHE- TDENRAKVEVT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLG DTAWDFGSVGGVFNSLGKGVHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFLS- TAVSA isol-ARB7701.AHF49785.1.Central_African_Republic/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 21 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDIGHE- TDENRAKVEVT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLG DTAWDFGSVGGVFNSLGKGVHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFLS- TAVSA isol-ArD_41519.AEN75266.1.Senegal/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 22 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEVT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLG DTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFLS- TAVSA MR766-NIID.BAP47441.1.Uganda/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 23 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMTVNDIGYE- TDENRAKVEVT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGKLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTV- EVQYAGTDGPCK IPVQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFLST- AVSA LC002520.1/326-829 Zika virus genomic RNA, strain: MR766-NIID, Uganda, Flavivirus envelope glycoprotein E. SEQ ID NO: 24 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMTVNDIGYE- TDENRAKVEVT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGKLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTV- EVQYAGTDGPCK IPVQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFLST- AVSA isol-MR_766.AEN75263.1.Uganda/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 25 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGYE- TDENRAKVEVT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGKLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTV- EVQYAGTDGPCK IPVQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFLST- AVSA ArD7117.AHL43501.1.-/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 26 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDIGHE-

TDENRAKVEVT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAVCTAAKVPAETLHGTVTV- EVQYAGTDGPC KVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEA- TVRGAKRMAVL GDTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFL- STAVSA AY632535.2/326-825 NC_012532.1 Zika virus strain MR 766, Uganda, Flavivirus envelope glycoprotein E. SEQ ID NO: 27 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIGYETDED- RAKVEVTPNSPR AEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAH- AKRQTVVVL GSQEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTVEVQY- AGTDGPCKIPVQ MAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGA- KRMAVLGDTAW DFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFLSTAVS- A MR_766.AAV34151.1.Uganda/291-790 Flavivirus envelope glycoprotein E. |Q32ZE1|Q32ZE1_9FL SEQ ID NO: 28 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIGYETDED- RAKVEVTPNSPR AEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAH- AKRQTVVVL GSQEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTVEVQY- AGTDGPCKIPVQ MAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGA- KRMAVLGDTAW DFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFLSTAVS- A MR_766.YP_009227198.1.Uganda/1-500 envelope protein E [Zika virus] SEQ ID NO: 29 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIGYETDED- RAKVEVTPNSPR AEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAH- AKRQTVVVL GSQEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTVEVQY- AGTDGPCKIPVQ MAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGA- KRMAVLGDTAW DFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFLSTAVS- A KU681081.3/308-811 Zika virus isolate Zika virus/H. sapiens-tc/THA/2014/ SV0127-14, Thailand, Flavivirus envelope glycoprotein E. SEQ ID NO: 30 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHTGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITEGTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLG DTAWDFGSVGGVLNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLS- TAVSA isol-Zika_virus % H. sapiens-tc % THA % 2014 % SV0127-_14.AMD61710.1.Thailand/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 31 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHTGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITEGTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLG DTAWDFGSVGGVLNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLS- TAVSA CK-ISL_2014.AIC06934.1.Cook_Islands/1-504 Flavivirus envelope glycoprotein E. (Fragment) OS = Zika virus GN = E PE = 4 SV = 1 SEQ ID NO: 32 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA Natal_RGN.AMB18850.1.Brazil:_Rio_Grande_do_Norte,_Natal/291-794 Flavivirus envelope glycoprotein E.] SEQ ID NO: 33 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA isol-Si323.AMC37200.1.Colombia/1-504 Flavivirus envelope glycoprotein E. SEQ ID NO: 34 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA KU707826.1/317-820 Zika virus isolate SSABR1, Brazil, Flavivirus envelope glycoprotein E. SEQ ID NO: 35 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA KU509998.1/326-829 Zika virus strain Haiti/1225/2014, Haiti, Flavivirus envelope glycoprotein E. SEQ ID NO: 36 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA isol-GDZ16001.AML82110.1.China/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 37 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA BeH819015.AMA12085.1.Brazil/291-794 Flavivirus envelope glycoprotein E.] SEQ ID NO: 38 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ

SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA MRS_OPY_Martinique_PaRi_2015.AMC33116.1.Martinique/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 39 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA KU501215.1/308-811 Zika virus strain PRVABC59, Puerto Rico, Flavivirus envelope glycoprotein E. SEQ ID NO: 40 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA Haiti % 1225%2014.AMB37295.1.Haiti/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 41 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA KU527068.1/308-811 Zika virus strain Natal RGN, Brazil: Rio Grande do Norte, Natal, Flavivirus envelope glycoprotein E. SEQ ID NO: 42 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA isol-Z1106027.ALX35662.1.Suriname/5-508 Flavivirus envelope glycoprotein E. SEQ ID NO: 43 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA isol-FLR.AMM39804.1.Colombia:_Barranquilla/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 44 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA PLCal_ZV_isol-From_Vero_E6_cells.AHL37808.1.Canada/254-757 Flavivirus envelope glycoprotein E. SEQ ID NO: 45 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA BeH818995.AMA12084.1.Brazil/291-794 Flavivirus envelope glycoprotein E. [Zika virus]. SEQ ID NO: 46 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA H/PF/2013.AHZ13508.1.French_Polynesia/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 47 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA PRVABC59.AMC13911.1.Puerto_Rico/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 48 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA KU321639.1/326-829 Zika virus strain ZikaSPH2015, Brazil, Flavivirus envelope glycoprotein E. SEQ ID NO: 49 IRCIGVSNRDFVEGMSGGTWVDIVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA ZikaSPH2015.ALU33341.1.Brazil/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 50 IRCIGVSNRDFVEGMSGGTWVDIVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ

SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA 103344.AMC13912.1.Guatemala/291-794 polyprotein [Zika virus]. 103344.AMC13912.1.Guatemala Flavivirus envelope glycoprotein E. SEQ ID NO: 51 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEIRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA isol-Brazil-ZKV2015.AMD16557.1.Brazil/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 52 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGTQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA KU497555.1/308-811 Zika virus isolate Brazil-ZKV2015, Flavivirus envelope glycoprotein E. SEQ ID NO: 53 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGTQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA isol-ZJ03.AMM39806.1.China/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 54 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGARRMAVLG DTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLS- TAVSA isol-FSS13025.AFD30972.1.Cambodia/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 55 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLMCLALGGVLIFLST- AVSA isol-Z1106032.ALX35660.1.Suriname/291-794 Flavivirus envelope glycoprotein E. [Zika virus] SEQ ID NO: 56 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNAKNGSISLMCLALGGVLIFLST- AVSA isol-Z1106033.ALX35659.1.Suriname/291-794 Flavivirus envelope glycoprotein E. [Zika virus] SEQ ID NO: 57 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNAKNGSISLMCLALGGVLIFLST- AVSA isol-BeH828305.AMK49165.1.Brazil/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 58 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDTQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA isol-GD01.AMK79468.1.China/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 59 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNGTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA isol-Z1106031.ALX35661.1.Suriname/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 60 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VLAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA ACD75819.1.Micronesia/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 61 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPAVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVLIFLST- AVSA KU681082.3/308-811 Zika virus isolate Zika virus/H. sapiens-tc/PHL/2012/CPC-0740, Philippines, Flavivirus envelope glycoprotein E. SEQ ID NO: 62 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC-

PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVLIFLST- AVSA isol-Zika_virus % H. sapiens-tc % PHL % 2012 % CPC-0740.AMD61711.1.Philippines/ 291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 63 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVLIFLST- AVSA isol-BeH823339.AMK49164.2.Brazil/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 64 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVSTTVSNMAEVRSYCYEATISDIASDSRC- PTQGEAYLDKQS DTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHET- DENRAKVEITP NSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEF- KDAHAKRQT AVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSA isol-P6-740.AEN75264.1.Malaysia/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 65 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDXGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTV- EVQYAGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWXRSGSTIGKAFEAT- VRGAKRMAVLG DTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVLIFLS- TAVSA KU744693.1/326-829 Zika virus isolate VE_Ganxian, China, Flavivirus envelope glycoprotein E. SEQ ID NO: 66 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTAMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMLVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLAHKEWFHDIPLPWHAGAATGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETVDGTVTV- EGQYGGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIIGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSG isol-VE_Ganxian.AMK79469.1.China/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 67 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTAMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMLVNDTGHE- TDENRAKVEIT PNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLAHKEWFHDIPLPWHAGAATGTPHWNNKEALVE- FKDAHAKRQT VVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETVDGTVTV- EGQYGGTDGPCK VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEAT- VRGAKRMAVLGD TAWDFGSVGGALNSLGKGIHQIIGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLST- AVSG ArD157995.AHL43503.1.-/291-794 Flavivirus envelope glycoprotein E. SEQ ID NO: 68 ISCIGVSNRDLVEGMSGGTWVDVVLEHGGCVTEMAQDKPTVDIELVTMTVSNMAEVRSYCYEASLSDMASASRC- PTQGEPSLDK QSDTQSVCKRTLGDRGWGNGCGIFGKGSLVTCSKFTCCKKMPGKSIQPENLEYRIMLPVHGSQHSGMIVNDIGH- ETDENRAKVEV TPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALV- EFKDAHAKRQ TVVVLGSQEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVT- VEVQSAGTDGPC KVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEA- TVRGAKRMAVL GDTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFL- STAVSA MR_766.ABI54475.1.Uganda/291-788 Flavivirus envelope glycoprotein E. SEQ ID NO: 69 IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRC- PTQGEAYLDKQ SDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDENRA- KVEVTPNSPRA EATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHA- KRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTVEVQYAG- TDGPCKVPAQM AVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAK- RMAVLGDTAWDF GSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGGVMIFLSTAVSA 5'-(dldC).sub.13-3' SEQ ID NO: 70 dldC dldC dldC dldC dldC dldC dldC dldC dldC dldC dldC dldC dldC KLK peptide SEQ ID NO: 71 KLKLLLLLKLK

Provided below are examples of nucleic acid sequences of the genomes of Chikungunya, Japanese Encephalitis and yellow fever viruses that may be used in the methods, compositions, and/or vaccines described herein.

TABLE-US-00007 Chikungunya virus strain LR2006_OPY1, complete genome ACCESSION: DQ443544 SEQ ID NO: 72 ATGGCTGCGTGAGACACACGTAGCCTACCAGTTTCTTACTGCTCTACTCTGCAAAGCAAGAGATTAATAACCCA- TCATGGATC CTGTGTACGTGGACATAGACGCTGACAGCGCCTTTTTGAAGGCCCTGCAACGTGCGTACCCCATGTTTGAGGTG- GAACCAA GGCAGGTCACACCGAATGACCATGCTAATGCTAGAGCGTTCTCGCATCTAGCTATAAAACTAATAGAGCAGGAA- ATTGACCC CGACTCAACCATCCTGGATATCGGCAGTGCGCCAGCAAGGAGGATGATGTCGGACAGGAAGTACCACTGCGTCT- GCCCGAT GCGCAGTGCGGAAGATCCCGAGAGACTCGCCAATTATGCGAGAAAGCTAGCATCTGCCGCAGGAAAAGTCCTGG- ACAGAA ACATCTCTGGAAAGATCGGGGACTTACAAGCAGTAATGGCCGTGCCAGACACGGAGACGCCAACATTCTGCTTA- CACACAG ACGTCTCATGTAGACAGAGAGCAGACGTCGCTATATACCAAGACGTCTATGCTGTACACGCACCCACGTCGCTA- TACCACCA GGCGATTAAAGGGGTCCGAGTGGCGTACTGGGTTGGGTTCGACACAACCCCGTTCATGTACAATGCCATGGCGG- GTGCCTA CCCCTCATACTCGACAAACTGGGCAGATGAGCAGGTACTGAAGGCTAAGAACATAGGATTATGTTCAACAGACC- TGACGGA AGGTAGACGAGGCAAGTTGTCTATTATGAGAGGGAAAAAGCTAAAACCGTGCGACCGTGTGCTGTTCTCAGTAG- GGTCAAC GCTCTACCCGGAAAGCCGCAAGCTACTTAAGAGCTGGCACCTGCCATCGGTGTTCCATTTAAAGGGCAAACTCA- GCTTCACA TGCCGCTGTGATACAGTGGTTTCGTGTGAGGGCTACGTCGTTAAGAGAATAACGATGAGCCCAGGCCTTTATGG- AAAAACC ACAGGGTATGCGGTAACCCACCACGCAGACGGATTCCTGATGTGCAAGACTACCGACACGGTTGACGGCGAAAG- ARTGTCA TTCTCGGTGTGCACATACGTGCCGGCGACCATTTGTGATCAAATGACCGGCATCCTTGCTACAGAAGTCACGCC- GGAGGATG CACAGAAGCTGTTGGTGGGGCTGAACCAGAGAATAGTGGTTAACGGCAGAACGCAACGGAATACGAACACCATG- AAAAAT TATCTGCTTCCCGTGGTCGCCCAAGCCTTCAGTAAGTGGGCAAAGGAGTGCCGGAAAGACATGGAAGATGAAAA- ACTCCTG GGGGTCAGAGAAAGAACACTGACCTGCTGCTGTCTATGGGCATTCAAGAAGCAGAAAACACACACGGTCTACAA- GAGGCCT GATACCCAGTCAATTCAGAAGGTTCAGGCCGAGTTTGACAGCTTTGTGGTACCGAGTCTGTGGTCGTCCGGGTT- GTCAATCC CTTTGAGGACTAGAATCAAATGGTTGTTAAGCAAGGTGCCAAAAACCGACCTGATCCCATACAGCGGAGACGCC- CGAGAAG CCCGGGACGCAGAAAAAGAAGCAGAGGAAGAACGAGAAGCAGAACTGACTCGCGAAGCCCTACCACCTCTACAG- GCAGCA CAGGAAGATGTTCAGGTCGAAATCGACGTGGAACAGCTTGAGGACAGAGCGGGCGCAGGAATAATAGAGACTCC- GAGAG GAGCTATCAAAGTTACTGCCCAACCAACAGACCACGTCGTGGGAGAGTACCTGGTACTCTCCCCGCAGACCGTA- CTACGTAG CCAGAAGCTCAGTCTGATTCACGCTTTGGCGGAGCAAGTGAAGACGTGCACGCACAACGGACGAGCAGGGAGGT- ATGCGG TCGAAGCGTACGACGGCCGAGTCCTAGTGCCCTCAGGCTATGCAATCTCGCCTGAAGACTTCCAGAGTCTAAGC- GAAAGCG CAACGATGGTGTATAACGAAAGAGAGTTCGTAAACAGAAAGCTACACCATATTGCGATGCACGGACCAGCCCTG- AACACCG ACGAAGAGTCGTATGAGCTGGTGAGGGCAGAGAGGACAGAACACGAGTACGTCTACGACGTGGATCAGAGAAGA- TGCTG TAAGAAGGAAGAAGCCGCAGGACTGGTACTGGTGGGCGACTTGACTAATCCGCCCTACCACGAATTCGCATATG- AAGGGCT AAAAATCCGCCCTGCCTGCCCATACAAAATTGCAGTCATAGGAGTCTTCGGAGTACCGGGATCTGGCAAGTCAG- CTATTATC AAGAACCTAGTTACCAGGCAGGACCTGGTGACTAGCGGAAAGAAAGAAAACTGCCAAGAAATCACCACCGACGT- GATGAG ACAGAGAGGTCTAGAGATATCTGCACGTACGGTTGACTCGCTGCTCTTGAATGGATGCAACAGACCAGTCGACG- TGTTGTA CGTAGACGAGGCGTTTGCGTGCCACTCTGGAACGCTACTTGCTTTGATCGCCTTGGTGAGACCAAGGCAGAAAG- TTGTACTT TGTGGTGACCCGAAGCAGTGCGGCTTCTTCAATATGATGCAGATGAAAGTCAACTATAATCACAACATCTGCAC- CCAAGTGT ACCACAAAAGTATCTCCAGGCGGTGTACACTGCCTGTGACCGCCATTGTGTCATCGTTGCATTACGAAGGCAAA- ATGCGCAC TACGAATGAGTACAACAAGCCGATTGTAGTGGACACTACAGGCTCAACAAAACCTGACCCTGGAGACCTCGTGT- TAACGTG CTTCAGAGGGTGGGTTAAACAACTGCAAATTGACTATCGTGGATACGAGGTCATGACAGCAGCCGCATCCCAAG- GGTTAAC CAGAAAAGGAGTTTACGCAGTTAGACAAAAAGTTAATGAAAACCCGCTCTATGCATCAACGTCAGAGCACGTCA- ACGTACTC CTAACGCGTACGGAAGGTAAACTGGTATGGAAGACACTTTCCGGCGACCCGTGGATAAAGACGCTGCAGAACCC- ACCGAAA GGAAACTTCAAAGCAACTATTAAGGAGTGGGAGGTGGAGCATGCATCAATAATGGCGGGCATCTGCAGTCACCA- AATGAC CTTCGATACATTCCAAAATAAAGCCAACGTTTGTTGGGCTAAGAGCTTGGTCCCTATCCTCGAAACAGCGGGGA- TAAAACTA AATGATAGGCAGTGGTCTCAGATAATTCAAGCCTTCAAAGAAGACAAAGCATACTCACCTGAAGTAGCCCTGAA- TGAAATAT GTACGCGCATGTATGGGGTGGATCTAGACAGCGGGCTATTTTCTAAACCGTTGGTGTCTGTGTATTACGCGGAT- AACCACTG GGATAATAGGCCTGGAGGGAAAATGTTCGGATTTAACCCCGAGGCAGCATCCATTCTAGAAAGAAAGTATCCAT- TCACAAA AGGGAAGTGGAACATCAACAAGCAGATCTGCGTGACTACCAGGAGGATAGAAGACTTTAACCCTACCACCAACA- TCATACC GGCCAACAGGAGACTACCACACTCATTAGTGGCCGAACACCGCCCAGTAAAAGGGGAAAGAATGGAATGGCTGG- TTAACA AGATAAACGGCCACCACGTGCTCCTGGTCAGTGGCTATAACCTTGCACTGCCTACTAAGAGAGTCACTTGGGTA- GCGCCGTT AGGTGTCCGCGGAGCGGACTACACATACAACCTAGAGTTGGGTCTGCCAGCAACGCTTGGTAGGTATGACCTAG- TGGTCAT AAACATCCACACACCTTTTCGCATACACCATTACCAACAGTGCGTCGACCACGCAATGAAACTGCAAATGCTCG- GGGGTGAC TCATTGAGACTGCTCAAACCGGGCGGCTCTCTATTGATCAGAGCATATGGTTACGCAGATAGAACCAGTGAACG- AGTCATCT GCGTATTGGGACGCAAGTTTAGATCGTCTAGAGCGTTGAAACCACCATGTGTCACCAGCAACACTGAGATGTTT- TTCCTATTC AGCAACTTTGACAATGGCAGAAGGAATTTCACAACTCATGTCATGAACAATCAACTGAATGCAGCCTTCGTAGG- ACAGGTCA CCCGAGCAGGATGTGCACCGTCGTACCGGGTAAAACGCATGGACATCGCGAAGAACGATGAAGAGTGCGTAGTC- AACGCC GCTAACCCTCGCGGGTTACCGGGTGRCGGTGTTTGCAAGGCAGTATACAAAAAATGGCCGGAGTCCTTTAAGAA- CAGTGCA ACACCAGTGGGAACCGCAAAAACAGTTATGTGCGGTACGTATCCAGTAATCCACGCTGTTGGACCAAACTTCTC- TAATTATT CGGAGTCTGAAGGGGACCGGGAATTGGCAGCTGCCTATCGAGAAGTCGCAAAGGAAGTAACTAGGCTGGGAGTA- AATAGT GTAGCTATACCTCTCCTCTCCACAGGTGTATACTCAGGAGGGAAAGACAGGCTGACCCAGTCACTGAACCACCT- CTTTACAG CCATGGACTCGACGGATGCAGACGTGGTCATCTACTGCCGCGACAAAGAATGGGAGAAGAAAATATCTGAGGCC- ATACAG ATGCGGACCCAAGTAGAGCTGCTGGATGAGCACATCTCCATAGACTGCGATATTGTTCGCGTGCACCCTGACAG- CAGCTTG GCAGGCAGAAAAGGATACAGCACCACGGAAGGCGCACTGTACTCATATCTAGAAGGGACCCGTTTTCATCAGAC- GGCTGTG GATATGGCGGAGATACATACTATGTGGCCAAAGCAAACAGAGGCCAATGAGCAAGTCTGCCTATATGCCCTGGG- GGAAAG TATTGAATCGATCAGGCAGAAATGCCCGGTGGATGATGCAGACGCATCATCTCCCCCCAAAACTGTCCCGTGCC- TTTGCCGT TACGCTATGACTCCAGAACGCGTCACCCGGCTTCGCATGAACCACGTCACAAGCATAATTGTGTGTTCTTCGTT- TCCCCTCCC AAAGTACAAAATAGAAGGAGTGCAAAAAGTCAAATGCTCTAAGGTAATGCTATTTGACCACAACGTGCCATCGC- GCGTAAG TCCAAGGGAATATAKATCTTCCCAGGAGTCTGCACAGGAGGCGAGTACAATCACGTCACTGACGCATAGTCAAT- TCGACCTA AGCGTTGATGGCGAGATACTGCCCGTCCCGTCAGACCTGGATGCTGACGCCCCAGCCCTAGAACCAGCACTAGA- CGACGGG GCGACACACACGCTGCCATCCACAACCGGAAACCTTGCGGCCGTGTCTGATTGGGTAATGAGCACCGTACCTGT- CGCGCCG CCCAGAAGAAGGCGAGGGAGAAACCTGACTGTGACATGTGACGAGAGAGAAGGGAATATAACACCCATGGCTAG- CGTCCG ATTCTTTAGGGCAGAGCTGTGTCCGGTCGTACAAGAAACAGCGGAGACGCGTGACACAGCAATGTCTCTTCAGG- CACCACC GAGTACCGCCACGGAACCGAATCATCCGCCGATCTCCTTCGGAGCATCAAGCGAGACGTTCCCCATTACATTTG- GGGACTTC AACGAAGGAGAAATCGAAAGCTTGTCTTCTGAGCTACTAACTTTCGGAGACTTCTTACCAGGAGAAGTGGATGA- CTTGACA GACAGCGACTGGTCCACGTGCTCAGACACGGACGACGAGTTATGACTAGACAGGGCAGGTGGGTATATATTCTC- GTCGGAC ACCGGTCCAGGTCATTTACAACAGAAGTCAGTACGCCAGTCAGTGCTGCCGGTGAACACCCTGGAGGAAGTCCA- CGAGGAG AAGTGTTACCCACCTAAGCTGGATGAAGCAAAGGAGCAACTATTACTTAAGAAACTCCAGGAGAGTGCATCCAT- GGCCAAC AGAAGCAGGTATCAGTCGCGCAAAGTAGAAAACATGAAAGCAGCAATCATCCAGAGACTAAAGAGAGGCTGTAG- ACTATA CTTAATGTCAGAGACCCCAAAAGTCCCTACTTACCGGACTACATATCCGGCGCCTGTGTACTCGCCTCCGATCA- ACGTCCGAT TGTCCAATCCCGAGTCCGCAGTGGCAGCATGCAATGAGTTCTTAGCTAGAAACTATCCAACTGTCTCATCATAC- CAAATTACC GACGAGTATGATGCATATCTAGACATGGTGGACGGGTCGGAGAGTTGCCTGGACCGAGCGACATTCAATCCGTC- AAAACTC AGGAGCTACCCGAAACAGCACGCTTACCACGCGCCCTCCATCAGAAGCGCTGTACCGTCCCCATTCCAGAACAC- ACTACAGA ATGTACTGGCAGCAGCCACGAAAAGAAACTGCAACGTCACACAGATGAGGGAATTACCCACTTTGGACTCAGCA- GTATTCA ACGTGGAGTGTTTCAAAAAATTCGCATGCAACCAAGAATACTGGGAAGAATTTGCTGCCAGCCCTATTAGGATA- ACAACTGA GAATTTAGCAACCTATGTTACTAAACTAAAAGGGCCAAAAGCAGCAGCGCTATTCGCAAAAACCCATAATCTAC- TGCCACTA CAGGAAGTACCAATGGATAGGTTCACAGTAGATATGAAAAGGGACGTAAAGGTGACTCCTGGTACAAAGCATAC- AGAGGA AAGACCTAAGGTGCAGGTTATACAGGCGGCTGAACCCTTGGCGACAGCATACCTATGTGGGATTCACAGAGAGC- TGGTTAG GAGGCTGAACGCCGTCCTCCTACCCAATGTACATACACTATTTGACATGTCTGCCGAGGATTTCGATGCCATCA-

TAGCCGCAC ACTTTAAGCCAGGAGACACTGTTTTGGAAACGGACATAGCCTCCTTTGATAAGAGCCAAGATGATTCACTTGCG- CTTACTGC TTTGATGCTGTTAGAGGATTTAGGGGTGGATCACTCCCTGCTGGACTTGATAGAGGCTGCTTTCGGAGAGATTT- CCAGCTGT CACCTACCGACAGGTACGCGCTTCAAGTTCGGCGCCATGATGAAATCAGGTATGTTCCTAACTCTGTTCGTCAA- CACATTGTT AAACATCACCATCGCCAGCCGAGTGCTGGAAGATCGTCTGACAAAATCCGCGTGCGCGGCCTTCATCGGCGACG- ACAACAT AATACATGGAGTCGTCTCCGATGAATTGATGGCAGCCAGATGTGCCACTTGGATGAACATGGAAGTGAAGATCA- TAGATGC AGTTGTATCCTTGAAAGCCCCTTACTTTTGTGGAGGGTTTATACTGCACGATACTGTGACAGGAACAGCTTGCA- GAGTGGCA GACCCGCTAAAAAGGCTTTTTAAACTGGGCAAACCGCTAGCGGCAGGTGACGAACAAGATGAAGATAGAAGACG- AGCGCT GGCTGACGAAGTGATCAGATGGCAACGAACAGGGCTAATTGATGAGCTGGAGAAAGCGGTATACTCTAGGTACG- AAGTGC AGGGTATATCAGTTGTGGTAATGTCCATGGCCACCTTTGCAAGCTCCAGATCCAACTTCGAGAAGCTCAGAGGA- CCCGTCAT AACTTTGTACGGCGGTCCTAAATAGGTACGCACTACAGCTACCTATTTTGCAGAAGCCGACAGCAAGTATCTAA- ACACTAAT CAGCTACAATGGAGTTCATCCCAACCCAAACTTTTTACAATAGGAGGTACCAGCCTCGACCCTGGACTCCGCGC- CCTACTATC CAAGTCATCAGGCCCAGACCGCGCCCTCAGAGGCAAGCTGGGCAACTTGCCCAGCTGATCTCAGCAGTTAATAA- ACTGACA ATGCGCGCGGTACCCCAACAGAAGCCACGCAGGAATCGGAAGAATAAGAAGCAAAAGCAAAAACAACAGGCGCC- ACAAAA CAACACAAATCAAAAGAAGCAGCCACCTAAAAAGAAACCGGCTCAAAAGAAAAAGAAGCCGGGCCGCAGAGAGA- GGATG TGCATGAAAATCGAAAATGATTGTATTTTCGAAGTCAAGCACGAAGGTAAGGTAACAGGTTACGCGTGCCTGGT- GGGGGAC AAAGTAATGAAACCAGCACACGTAAAGGGGACCATCGATAACGCGGACCTGGCCAAACTGGCCTTTAAGCGGTC- ATCTAAG TATGACCTTGAATGCGCGCAGATACCCGTGCACATGAAGTCCGACGCTTCGAAGTTCACCCATGAGAAACCGGA- GGGGTAC TACAACTGGCACCACGGAGCAGTACAGTACTCAGGAGGCCGGTTCACCATCCCTACAGGTGCTGGCAAACCAGG- GGACAGC GGCAGACCGATCTTCGACAACAAGGGACGCGTGGTGGCCATAGTCTTAGGAGGAGCTAATGAAGGAGCCCGTAC- AGCCCT CTCGGTGGTGACCTGGAATAAAGACATTGTCACTAAAATCACCCCCGAGGGGGCCGAAGAGTGGAGTCTTGCCA- TCCCAGT TATGTGCCTGTTGGCAAACACCACGTTCCCCTGCTCCCAGCCCCCTTGCACGCCCTGCTGCTACGAAAAGGAAC- CGGAGGAA ACCCTACGCATGCTTGAGGACAACGTCATGAGACCTGGGTACTATCAGCTGCTACAAGCATCCTTAACATGTTC- TCCCCACCG CCAGCGACGCAGCACCAAGGACAACTTCAATGTCTATAAAGCCACAAGACCATACTTAGCTCACTGTCCCGACT- GTGGAGAA GGGCACTCGTGCCATAGTCCCGTAGCACTAGAACGCATCAGAAATGAAGCGACAGACGGGACGCTGAAAATCCA- GGTCTCC TTGCAAATCGGAATAAAGACGGATGACAGCCACGATTGGACCAAGCTGCGTTATATGGACAACCACATGCCAGC- AGACGCA GAGAGGGCGGGGCTATTTGTAAGAACATCAGCACCGTGTACGATTACTGGAACAATGGGACACTTCATCCTGGC- CCGATGT CCAAAAGGGGAAACTCTGACGGTGGGATTCACTGACAGTAGGAAGATTAGTCACTCATGTACGCACCCATTTCA- CCACGAC CCTCCTGTGATAGGTCGGGAAAAATTCCATTCCCGACCGCAGCACGGTAAAGAGCTACCTTGCAGCACGTACGT- GCAGAGC ACCGCCGCAACTACCGAGGAGATAGAGGTACACATGCCCCCAGACACCCCTGATCGCACATTAATGTCACAACA- GTCCGGC AACGTAAAGATCACAGTCAATGGCCAGACGGTGCGGTACAAGTGTAATTGCGGTGGCTCAAATGAAGGACTAAC- AACTACA GACAAAGTGATTAATAACTGCAAGGTTGATCAATGTCATGCCGCGGTCACCAATCACAAAAAGTGGCAGTATAA- CTCCCCTC TGGTCCCGCGTAATGCTGAACTTGGGGACCGAAAAGGAAAAATTCACATCCCGTTTCCGCTGGCAAATGTAACA- TGCAGGG TGCCTAAAGCAAGGAACCCCACCGTGACGTACGGGAAAAACCAAGTCATCATGCTACTGTATCCTGACCACCCA- ACACTCCT GTCCTACCGGAATATGGGAGAAGAACCAAACTATCAAGAAGAGTGGGTGATGCATAAGAAGGAAGTCGTGCTAA- CCGTGC CGACTGAAGGGCTCGAGGTCACGTGGGGCAACAACGAGCCGTATAAGTATTGGCCGCAGTTATCTACAAACGGT- ACAGCCC ATGGCCACCCGCATGAGATAATTCTGTATTATTATGAGCTGTACCCCACTATGACTGTAGTAGTTGTGTCAGTG- GCCACGTTC ATACTCCTGTCGATGGTGGGTATGGCAGCGGGGATGTGCATGTGTGCACGACGCAGATGCATCACACCGTATGA- ACTGACA CCAGGAGCTACCGTCCCTTTCCTGCTTAGCCTAATATGCTGCATCAGAACAGCTAAAGCGGCCACATACCAAGA- GGCTGCGA TATACCTGTGGAACGAGCAGCAACCTTTGTTTTGGCTACAAGCCCTTATTCCGCTGGCAGCCCTGATTGTTCTA- TGCAACTGT CTGAGACTCTTACCATGCTGCTGTAAAACGTTGGCTTTTTTAGCCGTAATGAGCGTCGGTGCCCACACTGTGAG- CGCGTACG AACACGTAACAGTGATCCCGAACACGGTGGGAGTACCGTATAAGACTCTAGTCAATAGACCTGGCTACAGCCCC- ATGGTATT GGAGATGGAACTACTGTCAGTCACTTTGGAGCCAACACTATCGCTTGATTACATCACGTGCGAGTACAAAACCG- TCATCCCG TCTCCGTACGTGAAGTGCTGCGGTACAGCAGAGTGCAAGGACAAAAACCTACCTGACTACAGCTGTAAGGTCTT- CACCGGC GTCTACCCATTTATGTGGGGCGGCGCCTACTGCTTCTGCGACGCTGAAAACACGCAGTTGAGCGAAGCACACGT- GGAGAAG TCCGAATCATGCAAAACAGAATTTGCATCAGCATACAGGGCTCATACCGCATCTGCATCAGCTAAGCTCCGCGT- CCTTTACCA AGGAAATAACATCACTGTAACTGCCTATGCAAACGGCGACCATGCCGTCACAGTTAAGGACGCCAAATTCATTG- TGGGGCC AATGTCTTCAGCCTGGACACCTTTCGACAACAAAATTGTGGTGTACAAAGGTGACGTCTATAACATGGACTACC- CGCCCTTTG GCGCAGGAAGACCAGGACAATTTGGCGATATCCAAAGTCGCACACCTGAGAGTAAAGACGTCTATGCTAATACA- CAACTGG TACTGCAGAGACCGGCTGTGGGTACGGTACACGTGCCATACTCTCAGGCACCATCTGGCTTTAAGTATTGGCTA- AAAGAACG CGGGGCGTCGCTGCAGCACACAGCACCATTTGGCTGCCAAATAGCAACAAACCCGGTAAGAGCGGTGAACTGCG- CCGTAG GGAACATGCCCATCTCCATCGACATACCGGAAGCGGCCTTCACTAGGGTCGTCGACGCGCCCTCTTTAACGGAC- ATGTCGTG CGAGGTACCAGCCTGCACCCATTCCTCAGACTTTGGGGGCGTCGCCATTATTAAATATGCAGCCAGCAAGAAAG- GCAAGTG TGCGGTGCATTCGATGACTAACGCCGTCACTATTCGGGAAGCTGAGATAGAAGTTGAAGGGAATTCTCAGCTGC- AAATCTCT TTCTCGACGGCCTTAGCCAGCGCCGAATTCCGCGTACAAGTCTGTTCTACACAAGTACACTGTGCAGCCGAGTG- CCACCCCC CGAAGGACCACATAGTCAACTACCCGGCGTCACATACCACCCTCGGGGTCCAGGACATCTCCGCTACGGCGATG- TCATGGG TGCAGAAGATCACGGGAGGTGTGGGACTGGTTGTTGCTGTTGCCGCACTGATTCTAATCGTGGTGCTATGCGTG- TCGTTCA GCAGGCACTAACTTGACAATTAAGTATGAAGGTATATGTGTCCCCTAAGAGACACACTGTACATAGCAAATAAT- CTATAGAT CAAAGGGCTACGCAACCCCTGAATAGTAACAAAATACAAAATCACTAAAAATTATAAAAACAGAAAAATACATA- AATAGGT ATACGTGTCCCCTAAGAGACACATTGTATGTAGGTGATAAGTATAGATCAAAGGGCCGAATAACCCCTGAATAG- TAACAAA ATATGAAAATCAATAAAAATCATAAAATAGAAAAACCATAAACAGAAGTAGTTCAAAGGGCTATAAAACCCCTG- AATAGTA ACAAAACATAAAATTAATAAAAATCAAATGAATACCATAATTGGCAAACGGAAGAGATGTAGGTACTTAAGCTT- CCTAAAAG CAGCCGAACTCACTTTGAGAAGTAGGCATAGCATACCGAACTCTTCCACGATTCTCCGAACCCACAGGGACGTA- GGAGATGT TATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA Japanese encephalitis virus strain SA14-14-2, complete genome, ACCESSION: KC517497 SEQ ID NO: 73 TTTAAACAGTTTTTTAGAACGGAAGATAACCATGACTAAAAAACCAGGAGGGCCCGGTAAAAACCGGGCTATCA- ATATGCT GAAACGCGGCCTACCCCGCGTATTCCCACTAGTGGGAGTGAAGAGGGTAGTAATGAGCTTGTTGGACGGCAGAG- GGCCAG TACGTTTCGTGCTGGCTCTTATCACGTTCTTCAAGTTTACAGCATTAGCCCCGACCAAGGCGCTTTTAGGCCGA- TGGAAAGCA GTGGAAAAGAGTGTGGCAATGAAACATCTTACTAGTTTCAAACGAGAACTTGGAACACTCATTGACGCCGTGAA- CAAGCGG GGCAGAAAGCAAAACAAAAGAGGAGGAAATGAAGGCTCAATCATGTGGCTCGCGAGCTTGGCAGTTGTCATAGC- TTGTGC AGGAGCCATGAAGTTGTCGAATTTCCAGGGGAAGCTTTTGATGACCATCAACAACACGGACATTGCAGACGTTA- TCGTGATT CCCACCTCAAAAGGAGAGAACAGATGCTGGGTCCGGGCAATCGACGTCGGCTACATGTGTGAGGACACTATCAC- GTACGAA TGTCCTAAGCTTACCATGGGCAATGATCCAGAGGATGTGGATTGCTGGTGTGACAACCAAGAAGTCTACGTCCA- ATATGGA CGGTGCACGCGGACCAGGCATTCCAAGCGAAGCAGGAGATCCGTGTCGGTCCAAACACATGGGGAGAGTTCACT- AGTGAA TAAAAAAGAGGCTTGGCTGGATTCAACGAAAGCCACACGATATCTCATGAAAACTGAGAACTGGATCATAAGGA- ATCCTGG CTATGCTTTCCTGGCGGCGGTACTTGGCTGGATGCTTGGCAGTAACAACGGTCAACGCGTGGTATTTACCATCC- TCCTGCTGT TGGTCGCTCCGGCTTACAGTTTTAATTGTCTGGGAATGGGCAATCGTGACTTCATAGAAGGAGCCAGTGGAGCC- ACTTGGG TGGACTTGGTGCTAGAAGGAGATAGCTGCTTGACAATCATGGCAAACGACAAACCAACATTGGACGTCCGCATG- ATTAACA TCGAAGCTAGCCAACTTGCTGAGGTCAGAAGTTACTGCTATCATGCTTCAGTCACTGACATCTCGACGGTGGCT- CGGTGCCC CACGACTGGAGAAGCCCACAACGAGAAGCGAGCTGATAGTAGCTATGTGTGCAAACAAGGCTTCACTGACCGTG- GGTGGG GCAACGGATGTGGACTTTTCGGGAAGGGAAGCATTGACACATGTGCAAAATTCTCCTGCACCAGTAAAGCGATT- GGGAGAA CAATCCAGCCAGAAAACATCAAATACGAAGTTGGCATTTTTGTGCATGGAACCACCACTTCGGAAAACCATGGG- AATTATTC AGCGCAAGTTGGGGCGTCCCAGGCGGCAAAGTTTACAGTAACACCCAATGCTCCTTCGATAACCCTCAAACTTG- GTGACTAC GGAGAAGTCACACTGGACTGTGAGCCAAGGAGTGGACTGAACACTGAAGCGTTTTACGTCATGACCGTGGGGTC- AAAGTC ATTTCTGGTCCATAGGGAGTGGTTTCATGACCTCGCTCTCCCCTGGACGTCCCCTTCGAGCACAGCGTGGAGAA-

ACAGAGAA CTCCTCATGGAATTTGAAGGGGCGCACGCCACAAAACAGTCCGTTGTTGCTCTTGGGTCACAGGAAGGAGGCCT- CCATCAG GCGTTGGCAGGAGCCATCGTGGTGGAGTACTCAAGCTCAGTGAAGTTAACATCAGGCCACCTGAAATGTAGGCT- GAAAATG GACAAACTGGCTCTGAAAGGCACAACCTATGGCATGTGTACAGAAAAATTCTCGTTCGCGAAAAATCCGGCGGA- CACTGGT CACGGAACAGTTGTCATTGAACTCTCCTACTCTGGGAGTGATGGCCCCTGCAAAATTCCGATTGTTTCCGTTGC- GAGCCTCAA TGACATGACCCCCGTTGGGCGGCTGGTGACAGTGAACCCCTTCGTCGCGACTTCCAGTGCCAACTCAAAGGTGC- TGGTCGA GATGGAACCCCCCTTCGGAGACTCCTACATCGTAGTTGGAAGGGGAGACAAGCAGATCAACCACCATTGGCACA- AAGCTGG AAGCACGCTGGGCAAGGCCTTTTCAACAACTTTGAAGGGAGCTCAAAGACTGGCAGCGTTGGGCGACACAGCCT- GGGACTT TGGCTCTATTGGAGGGGTCTTCAACTCCATAGGAAAAGCCGTTCACCAAGTGTTTGGTGGTGCCTTCAGAACAC- TCTTTGGG GGAATGTCTTGGATCACACAAGGGCTAATGGGTGCCCTACTGCTCTGGATGGGCGTCAACGCACGAGACCGATC- AATTGCT TTGGCCTTCTTAGCCACAGGGGGTGTGCTCGTGTTCTTAGCGACCAATGTGCATGCTGACACTGGATGTGCCAT- TGACATCA CAAGAAAAGAGATGAGATGTGGAAGTGGCATCTTCGTGCACAACGACGTGGAAGCCTGGGTGGATAGGTATAAA- TATTTG CCAGAAACGCCCAGATCCCTAGCGAAGATCGTCCACAAAGCGCACAAGGAAGGCGTGTGCGGAGTCAGATCTGT- CACTAGA CTGGAGCACCAAATGTGGGAAGCCGTACGGGACGAATTGAACGTCCTGCTCAAAGAGAATGCAGTGGACCTCAG- TGTGGT TGTGAACAAGCCCGTGGGAAGATATCGCTCAGCCCCTAAACGCCTATCCATGACGCAAGAGAAGTTTGAAATGG- GCTGGAA AGCATGGGGAAAAAGCATTCTCTTTGCCCCGGAATTGGCTAACTCCACATTTGTCGTAGATGGACCTGAGACAA- AGGAATG CCCTGATGAGCACAGAGCTTGGAACAGCATGCAAATCGAAGACTTCGGCTTTGGCATCACATCAACCCGTGTGT- GGCTGAA AATTAGAGAGGAGAGCACTGACGAGTGTGATGGAGCGATCATAGGCACGGCTGTCAAAGGACATGTGGCAGTCC- ATAGTG ACTTGTCGTACTGGATTGAGAGTCGCTACAACGACACATGGAAACTTGAGAGGGCAGTCTTTGGAGAGGTCAAA- TCTTGCA CTTGGCCAGAGACACACACCCTTTGGGGAGATGATGTTGAGGAAAGTGAACTCATCATTCCGCACACCATAGCC- GGACCAA AAAGCAAGCACAATCGGAGGGAAGGGTATAAGACACAAAACCAGGGACCTTGGGATGAGAATGGCATAGTCTTG- GACTTT GATTATTGCCCAGGGACAAAAGTCACCATTACAGAGGATTGTGGCAAGAGAGGCCCTTCGGTCAGAACCACTAC- TGACAGT GGAAAGTTGATCACTGACTGGTGCTGTCGCAGTTGCTCCCTTCCGCCCCTACGATTCCGGACAGAAAATGGCTG- CTGGTACG GAATGGAAATCAGACCTGTTAGGCATGATGAAACAACACTCGTCAGATCACAGGTTGATGCTTTCAATGGTGAA- ATGGTTG ACCCTTTTCAGCTGGGCCTTCTGGTGATGTTTCTGGCCACCCAGGAGGTCCTTCGCAAGAGGTGGACGGCCAGA- TTGACCAT TCCTGCGGTTTTGGGGGCCCTACTTGTGCTGATGCTTGGGGGCATCACTTACACTGATTTGGCGAGGTATGTGG- TGCTAGTC GCTGCTGCTTTCGCAGAGGCCAACAGTGGAGGAGACGTCCTGCACCTTGCTTTGATTGCCGTTTTTAAGATCCA- ACCAGCAT TTCTAGTGATGAACATGCTTAGCACGAGATGGACGAACCAAGAAAACGTGGTTCTGGTCCTAGGGGCTGCCTTT- TTCCAATT GGCCTCAGTAGATCTGCAAATAGGAGTCCACGGAATCCTGAATGCCGCCGCTATAGCATGGATGATTGTCCGAG- CGATCAC CTTCCCCACAACCTCCTCCGTCACCATGCCAGTCTTAGCGCTTCTAACTCCGGGGATGAGGGCTCTATACCTAG- ACACTTACA GAATCATCCTCCTCGTCATAGGGATTTGCTCCCTGCTGCACGAGAGGAAAAAGACCATGGCAAAAAAGAAAGGA- GCTGTAC TCTTGGGCTTAGCGCTCACATCCACTGGATGGTTCTCGCCCACCACTATAGCTGCCGGACTAATGGTCTGCAAC- CCAAACAA GAAGAGAGGGTGGCCAGCTACTGAGTTTTTGTCGGCAGTTGGATTGATGTTTGCCATCGTAGGTGGTTTGGCCG- AGTTGGA TATTGAATCCATGTCAATACCCTTCATGCTGGCAGGTCTCATGGCAGTGTCCTACGTGGTGTCAGGAAAAGCAA- CAGATATG TGGCTTGAACGGGCCGCCGACATCAGCTGGGAGATGGATGCTGCAATCACAGGAAGCAGTCGGAGGCTGGATGT- GAAACT GGATGATGACGGAGATTTTCACTTGATTGATGATCCCGGTGTTCCATGGAAGGTCTGGGTCCTGCGCATGTCTT- GCATTGGC TTAGCCGCCCTCACGCCTTGGGCCATCGTTCCCGCCGCTTTCGGTTATTGGCTCACTTTAAAAACAACAAAAAG- AGGGGGCG TGTTTTGGGACACGCCATCCCCAAAACCTTGCTCAAAAGGAGACACCACTACAGGAGTCTACCGAATTATGGCT- AGAGGGAT TCTTGGCACTTACCAGGCCGGCGTCGGAGTCATGTACGAGAATGTTTTCCACACACTATGGCACACAACTAGAG- GAGCAGCC ATTATGAGTGGAGAAGGAAAATTGACGCCATACTGGGGTAGTGTGAGAGAAGACCGCATAGCTTACGGAGGCCC- ATGGAG GTTTGACCGAAAATGGAATGGAACAGATGACGTGCAAGTGATCGTGGTAGAACCGGGGAAGGCTGCAGTAAACA- TCCAGA CAAAACCAGGAGTGTTTCGGACTCCCTTCGGGGAGGTTGGGGCTGTTAGTCTGGATTACCCGCGAGGAACATCC- GGCTCAC CCATTCTGGATTCCAATGGAGACATTATAGGCCTATACGGCAATGGAGTTGAGCTTGGCGATGGCTCATACGTC- AGCGCCAT CGTGCAGGGTGACCGTCAGGAGGAACCAGTCCCAGAAGCTTACACCCCAAACATGTTGAGAAAGAGACAGATGA- CTGTGC TAGATTTGCACCCTGGTTCAGGGAAAACCAGGAAAATTCTGCCACAAATAATTAAGGACGCTATCCAGCAGCGC- CTAAGAAC AGCTGTGTTGGCACCGACGCGGGTGGTAGCAGCAGAAATGGCAGAAGCTTTGAGAGGGCTCCCAGTACGATATC- AAACTTC AGCAGTGCAGAGAGAGCACCAAGGGAATGAAATAGTGGATGTGATGTGCCACGCCACTCTGACCCATAGACTGA- TGTCACC GAACAGAGTGCCCAACTACAACCTATTTGTCATGGATGAAGCTCATTTCACCGACCCAGCCAGTATAGCCGCAC- GAGGATAC ATTGCTACCAAGGTGGAATTAGGGGAGGCAGCAGCCATCTTTATGACAGCGACCCCGCCTGGAACCACGGATCC- TTTTCCTG ACTCAAATGCCCCAATCCATGATTTGCAAGATGAGATACCAGACAGGGCATGGAGCAGTGGATACGAATGGATC- ACAGAAT ATGCGGGTAAAACCGTGTGGTTTGTGGCGAGCGTAAAAATGGGGAATGAGATTGCAATGTGCCTCCAAAGAGCG- GGGAAA AAGGTCATCCAACTCAACCGCAAGTCCTATGACACAGAATACCCAAAATGTAAGAATGGAGACTGGGATTTTGT- CATTACCA CCGACATCTCTGAAATGGGGGCCAACTTCGGTGCGAGCAGGGTCATCGACTGTAGAAAGAGCGTGAAACCCACC- ATCTTAG AAGAGGGAGAAGGCAGAGTCATCCTCGGAAACCCATCTCCCATAACCAGTGCAAGCGCAGCTCAACGGAGGGGC- AGAGTA GGCAGAAACCCCAACCAAGTTGGAGATGAATACCACTATGGGGGGGCTACCAGTGAAGATGACAGTAACCTAGC- CCATTG GACAGAGGCAAAGATCATGTTAGACAACATACACATGCCCAATGGACTGGTGGCCCAGCTCTATGGACCAGAGA- GGGAAA AGGCTTTCACAATGGATGGCGAATACCGTCTCAGAGGTGAAGAAAAGAAAAACTTCTTAGAGCTGCTTAGGACG- GCTGACC TCCCGGTGTGGCTGGCCTACAAGGTGGCGTCCAATGGCATTCAGTACACCGACAGAAAGTGGTGTTTTGATGGG- CCGCGTA CGAATGCCATACTGGAGGACAACACCGAGGTAGAGATAGTCACCCGGATGGGTGAGAGGAAAATCCTCAAGCCG- AGATGG CTTGATGCAAGAGTTTATGCAGATCACCAAGCCCTCAAGTGGTTCAAAGACTTTGCAGCAGGGAAGAGATCAGC- CGTTAGC TTCATAGAGGTGCTCGGTCGCATGCCTGAGCATTTCATGGGAAAGACGCGGGAAGCTTTAGACACCATGTACTT- GGTTGCA ACGGCTGAGAAAGGTGGGAAAGCACACCGAATGGCTCTCGAAGAGCTGCCAGATGCACTGGAAACCATCACACT- TATTGTC GCCATTACTGTGATGACAGGAGGATTCTTCCTACTAATGATGCAGCGAAAGGGTATAGGGAAGATGGGTCTTGG- AGCTCTA GTGCTCACGCTAGCTACCTTCTTCCTGTGGGCGGCAGAGGTTCCTGGAACCAAAATAGCAGGGACCCTGCTGAT- CGCCCTGC TGCTGATGGTGGTTCTCATCCCAGAACCGGAAAAACAGAGGTCACAGACAGATAACCAACTGGCGGTGTTTCTC- ATCTGTGT CTTGACCGTGGTTGGAGTGGTGGCAGCAAACGAGTACGGGATGCTAGAAAAAACCAAAGCAGATCTCAAGAGCA- TGTTTG GCGGAAAGACGCAGGCATCAGGACTGACTGGATTGCCAAGCATGGCACTGGACCTGCGTCCAGCCACAGCCTGG- GCACTG TATGGGGGGAGCACAGTCGTGCTAACCCCTCTTCTGAAGCACCTGATCACGTCGGAATACGTCACCACATCGCT- AGCCTCAA TTAACTCACAAGCTGGCTCATTATTCGTCTTGCCACGAGGCGTGCCTTTTACCGACCTAGACTTGACCGTTGGC- CTCGTCTTCC TTGGCTGTTGGGGTCAAATCACCCTCACAACGTTTCTGACAGCCATGGTTCTGGCGACACTTCACTATGGGTAC- ATGCTCCCT GGATGGCAAGCAGAAGCACTCAGGGCTGCCCAGAGAAGGACAGCGGCTGGAATAATGAAGAATGCCGTTGTTGA- CGGAA TGGTCGCCACTGATGTGCCTGAACTGGAAAGGACTACTCCTCTGATGCAAAAGAAAGTCGGACAGGTGCTCCTC- ATAGGGG TAAGCGTGGCAGCGTTCCTCGTCAACCCTAATGTCACCACTGTGAGAGAAGCAGGGGTGTTGGTGACGGCGGCT- ACGCTTA CTTTGTGGGACAATGGAGCCAGTGCCGTTTGGAATTCCACCACAGCCACGGGACTCTGCCATGTCATGCGAGGT- AGCTACCT GGCTGGAGGCTCCATTGCTTGGACTCTCATCAAGAACGCTGATAAGCCCTCCTTGAAAAGGGGAAGGCCTGGGG- GCAGGA CGCTAGGGGAGCAGTGGAAGGAAAAACTAAATGCCATGAGCAGAGAAGAGTTTTTTAAATACCGGAGAGAGGCC- ATAATC GAGGTGGACCGCACTGAAGCACGCAGGGCCAGACGTGAAAATAACATAGTGGGAGGACATCCGGTTTCGCGAGG- CTCAGC AAAACTCCGTTGGCTCGTGGAGAAAGGATTTGTCTCGCCAATAGGAAAAGTCATTGATCTAGGGTGTGGGCGTG- GAGGATG GAGCTACTACGCAGCAACCCTGAAGAAGGTCCAGGAAGTCAGAGGATACACGAAAGGTGGGGCGGGACATGAAG- AACCG ATGCTCATGCAGAGCTACGGCTGGAACCTGGTCTCCCTGAAGAGTGGAGTGGACGTGTTTTACAAACCTTCAGA- GCCCAGT GACACCCTGTTCTGTGACATAGGGGAATCCTCCCCAAGTCCAGAAGTAGAAGAACAACGCACACTACGCGTCCT- AGAGATG ACATCTGACTGGTTGCACCGAGGACCTAGAGAGTTCTGCATTAAAGTTCTCTGCCCTTACATGCCCAAGGTTAT- AGAAAAAA TGGAAGTTCTGCAGCGCCGCTTCGGAGGTGGGCTAGTGCGTCTCCCCCTGTCCCGAAACTCCAATCACGAGATG- TATTGGGT TAGTGGAGCCGCTGGCAATGTGGTGCACGCTGTGAACATGACCAGCCAGGTACTACTGGGGCGAATGGATCGCA- CAGTGT

GGAGAGGGCCAAAGTATGAGGAAGATGTCAACCTAGGGAGCGGAACAAGAGCCGTGGGAAAGGGAGAAGTCCAT- AGCA ATCAGGAGAAAATCAAGAAGAGAATCCAGAAGCTTAAAGAAGAATTCGCCACAACGTGGCACAAAGACCCTGAG- CATCCAT ACCGCACTTGGACATACCACGGAAGCTATGAAGTGAAGGCTACTGGCTCAGCCAGCTCTCTCGTCAACGGAGTG- GTGAAGC TCATGAGCAAACCTTGGGACGCCATTGCCAACGTCACCACCATGGCCATGACTGACACCACCCCTTTTGGACAG- CAAAGAGT TTTCAAGGAGAAAGTTGACACGAAGGCTCCTGAGCCACCAGCTGGAGCCAAGGAAGTGCTCAACGAGACCACCA- ACTGGCT GTGGGCCCACTTGTCACGGGAAAAAAGACCCCGCTTGTGCACCAAGGAAGAATTCATAAAGAAAGTCAACAGCA- ACGCGG CTCTTGGAGCAGTGTTCGCTGAACAGAATCAATGGAGCACGGCGCGTGAGGCTGTGGATGACCCGCGGTTTTGG- GAGATG GTTGATGAAGAGAGGGAAAACCATCTGCGAGGAGAGTGTCACACATGTATCTACAACATGATGGGAAAAAGAGA- GAAGAA GCCTGGAGAGTTTGGAAAAGCTAAAGGAAGCAGGGCCATTTGGTTCATGTGGCTTGGAGCACGGTATCTAGAGT- TTGAAGC TTTGGGGTTCCTGAATGAAGACCATTGGCTGAGCCGAGAGAATTCAGGAGGTGGAGTGGAAGGCTCAGGCGTCC- AAAAGC TGGGATACATCCTCCGTGACATAGCAGGAAAGCAAGGAGGGAAAATGTACGCTGATGATACCGCCGGGTGGGAC- ACTAGA ATTACCAGAACTGATTTAGAAAATGAAGCTAAGGTACTGGAGCTCCTAGACGGTGAACACCGCATGCTCGCCCG- AGCCATA ATTGAACTGACTTACAGGCACAAAGTGGTCAAGGTCATGAGACCTGCAGCAGAAGGAAAGACCGTGATGGACGT- GATATC AAGAGAAGATCAAAGGGGGAGTGGACAGGTGGTCACTTATGCTCTTAACACTTTCACGAACATCGCTGTCCAGC- TCGTCAG GCTGATGGAGGCTGAGGGGGTCATTGGACCACAACACTTGGAACAGCTACCTAGGAAAAACAAGATAGCTGTCA- GGACCT GGCTCTTTGAGAATGGAGAGGAGAGAGTGACCAGGATGGCGATCAGCGGAGACGACTGTGTCGTCAAGCCGCTG- GACGA CAGATTCGCCACAGCCCTCCACTTCCTCAACGCAATGTCAAAGGTCAGAAAAGACATCCAGGAATGGAAGCCTT- CGCATGGC TGGCACGATTGGCAGCAAGTTCCCTTCTGCTCTAACCATTTTCAGGAGATTGTGATGAAAGATGGAAGGAGTAT- AGTTGTCC CGTGCAGAGGACAGGATGAGCTGATAGGCAGGGCTCGCATCTCTCCAGGAGCTGGATGGAATGTGAAGGACACA- GCTTGC CTGGCCAAAGCATATGCACAGATGTGGCTACTCCTATACTTCCATCGCAGGGACTTGCGTCTCATGGCAAATGC- GATTTGCTC AGCAGTGCCAGTGGATTGGGTGCCCACAGGCAGGACATCCTGGTCAATACACTCGAAAGGAGAGTGGATGACCA- CGGAAG ACATGCTGCAGGTCTGGAACAGAGTCTGGATTGAAGAAAATGAATGGATGATGGACAAGACTCCAATCACAAGC- TGGACA GACGTTCCGTATGTGGGAAAGCGTGAGGACATCTGGTGTGGCAGCCTCATCGGAACGCGATCCAGAGCAACCTG- GGCTGA GAACATCTATGCGGCGATAAACCAGGTTAGAGCTGTCATTGGGAAAGAAAATTATGTTGACTACATGACCTCAC- TCAGGAG ATACGAAGACGTCTTGATCCAGGAAGACAGGGTCATCTAGTGTGATTTAAGGTAGAAAAGTAGACTATGTAAAT- AATGTAA ATGAGAAAATGCATGCATATGGAGTCAGGCCAGCAAAAGCTGCCACCGGATACTGGGTAGACGGTGCTGCCTGC- GTCTCAG TCCCAGGAGGACTGGGTTAACAAATCTGACAACAGAAAGTGAGAAAGCCCTCAGAACCGTCTCGGAAGTAGGTC- CCTGCTC ACTGGAAGTTGAAAGACCAACGTCAGGCCACAAATTTGTGCCACTCCGCTAGGGAGTGCGGCCTGCGCAGCCCC- AGGAGG ACTGGGTTACCAAAGCCGTTGAGGCCCCCACGGCCCAAGCCTCGTCTAGGATGCAATAGACGAGGTGTAAGGAC- TAGAGGT TAGAGGAGACCCCGTGGAAACAACAACATGCGGCCCAAGCCCCCTCGAAGCTGTAGAGGAGGTGGAAGGACTAG- AGGTTA GAGGAGACCCCGCATTTGCATCAAACAGCATATTGACACCTGGGAATAGACTGGGAGATCTTCTGCTCTATCTC- AACATCAG CTACTAG Japanese encephalitis virus strain SA14-14-2, complete genome, ACCESSION: JN604986 SEQ ID NO: 74 AGAAGTTTATCTGTGTGAACTTCTTGGCTTAGTATCGTAGAGAAGAATCGAGAGATTAGTGCAGTTTAAACAGT- TTTTTAGA ACGGAAGATAACCATGACTAAAAAACCAGGAGGGCCCGGTAAAAACCGGGCTATCAATATGCTGAAACGCGGCC- TACCCC GCGTATTCCCACTAGTGGGAGTGAAGAGGGTAGTAATGAGCTTGTTGGACGGCAGAGGGCCAGTACGTTTCGTG- CTGGCTC TTATCACGTTCTTCAAGTTTACAGCATTAGCCCCGACCAAGGCGCTTTCAGGCCGATGGAAAGCAGTGGAAAAG- AGTGTGG CAATGAAACATCTTACTAGTTTCAAACGAGAACTTGGAACACTCATTGACGCCGTGAACAAGCGGGGCAGAAAG- CAAAACA AAAGAGGAGGAAATGAAGGCTCAATCATGTGGCTCGCGAGCTTGGCAGTTGTCATAGCTTGTGCAGGAGCCATG- AAGTTGT CGAATTTCCAGGGGAAGCTTTTGATGACCATCAACAACACGGACATTGCAGACGTTATCGTGATTCCCACCTCA- AAAGGAGA GAACAGATGCTGGGTCCGGGCAATCGACGTCGGCTACATGTGTGAGGACACTATCACGTACGAATGTCCTAAGC- TTACCAT GGGCAATGATCCAGAGGATGTGGATTGCTGGTGTGACAACCAAGAAGTCTACGTCCAATATGGACGGTGCACGC- GGACCA GGCATTCCAAGCGAAGCAGGAGATCCGTGTCGGTCCAAACACATGGGGAGAGTTCACTAGTGAATAAAAAAGAG- GCTTGG CTGGATTCAACGAAAGCCACACGATATCTCATGAAAACTGAGAACTGGATCATAAGGAATCCTGGCTATGCTTT- CCTGGCGG CGGTACTTGGCTGGATGCTTGGCAGTAACAACGGTCAACGCGTGGTATTTACCATCCTCCTGCTGTTGGTCGCT- CCGGCTTA CAGTTTTAATTGTCTGGGAATGGGCAATCGTGACTTCATAGAAGGAGCCAGTGGAGCCACTTGGGTGGACTTGG- TGCTAGA AGGAGACAGCTGCTTGACAATCATGGCAAACGACAAACCAACATTGGACGTCCGCATGATTAACATCGAAGCTA- GCCAACT TGCTGAGGTCAGAAGTTACTGCTATCATGCTTCAGTCACTGACATCTCGACGGTGGCTCGGTGCCCCACGACTG- GAGAAGCC CACAACGAGAAGCGAGCTGATAGTAGCTATGTGTGCAAACAAGGCTTCACTGACCGTGGGTGGGGCAACGGATG- TGGATT TTTCGGGAAGGGAAGCATTGACACATGTGCAAAATTCTCCTGCACCAGTAAAGCGATTGGGAGAACAATCCAGC- CAGAAAA CATCAAATACAAAGTTGGCATTTTTGTGCATGGAACCACCACTTCGGAAAACCATGGGAATTATTCAGCGCAAG- TTGGGGCG TCCCAGGCGGCAAAGTTTACAGTAACACCCAATGCTCCTTCGGTAGCCCTCAAACTTGGTGACTACGGAGAAGT- CACACTGG ACTGTGAGCCAAGGAGTGGACTGAACACTGAAGCGTTTTACGTCATGACCGTGGGGTCAAAGTCATTTCTGGTC- CATAGGG AGTGGTTTCATGACCTCGCTCTCCCCTGGACGTCCCCTTCGAGCACAGCGTGGAGAAACAGAGAACTCCTCATG- GAATTTGA AGGGGCGCACGCCACAAAACAGTCCGTTGTTGCTCTTGGGTCACAGGAAGGAGGCCTCCATCATGCGTTGGCAG- GAGCCAT CGTGGTGGAGTACTCAAGCTCAGTGATGTTAACATCAGGCCACCTGAAATGTAGGCTGAAAATGGACAAACTGG- CTCTGAA AGGCACAACCTATGGCATGTGTACAGAAAAATTCTCGTTCGCGAAAAATCCGGTGGACACTGGTCACGGAACAG- TTGTCATT GAACTCTCCTACTCTGGGAGTGATGGCCCCTGCAAAATTCCGATTGTTTCCGTTGCGAGCCTCAATGACATGAC- CCCCGTTGG GCGGCTGGTGACAGTGAACCCCTTCGTCGCGACTTCCAGTGCCAACTCAAAGGTGCTGGTCGAGATGGAACCCC- CCTTCGG AGACTCCTACATCGTAGTTGGAAGGGGAGACAAGCAGATCAACCACCATTGGCACAAAGCTGGAAGCACGCTGG- GCAAGG CCTTTTCAACAACTTTGAAGGGAGCTCAAAGACTGGCAGCGTTGGGCGACACAGCCTGGGACTTTGGCTCTATT- GGAGGGG TCTTCAACTCCATAGGAAGAGCCGTTCACCAAGTGTTTGGTGGTGCCTTCAGAACACTCTTTGGGGGAATGTCT- TGGATCAC ACAAGGGCTAATGGGTGCCCTACTGCTCTGGATGGGCGTCAACGCACGAGACCGATCAATTGCTTTGGCCTTCT- TAGCCACA GGAGGTGTGCTCGTGTTCTTAGCGACCAATGTGCATGCTGACACTGGATGTGCCATTGACATCACAAGAAAAGA- GATGAGA TGTGGAAGTGGCATCTTCGTGCACAACGACGTGGAAGCCTGGGTGGATAGGTATAAATATTTGCCAGAAACGCC- CAGATCC CTAGCGAAGATCGTCCACAAAGCGCACAAGGAAGGCGTGTGCGGAGTCAGATCTGTCACTAGACTGGAGCACCA- AATGTG GGAAGCCGTAAGGGACGAATTGAACGTCCTGCTCAAAGAGAATGCAGTGGACCTCAGTGTGGTTGTGAACAAGC- CCGTGG GAAGATATCGCTCAGCCCCTAAACGCCTATCCATGACGCAAGAGAAGTTTGAAATGGGCTGGAAAGCATGGGGA- AAAAGC ATCCTCTTTGCCCCGGAATTGGCTAACTCCACATTTGTCGTAGATGGACCTGAGACAAAGGAATGCCCTGATGA- GCACAGAG CTTGGAACAGCATGCAAATCGAAGACTTCGGCTTTGGCATCACATCAACCCGTGTGTGGCTGAAAATTAGAGAG- GAGAGCA CTGACGAGTGTGATGGAGCGATCATAGGCACGGCTGTCAAAGGACATGTGGCAGTCCATAGTGACTTGTCGTAC- TGGATTG AGAGTCGCTACAACGACACATGGAAACTTGAGAGGGCAGTCTTTGGAGAGGTCAAATCTTGCACTTGGCCAGAG- ACACACA CCCTTTGGGGAGATGATGTTGAGGAAAGTGAACTCATCATTCCGCACACCATAGCCGGACCAAAAAGCAAGCAC- AATCGGA GGGAAGGGTATAAGACACAAAACCAGGGACCTTGGGATGAGAATGGCATAGTCTTGGACTTTGATTATTGCCCA- GGGACA AAAGTCACCATTACAGAGGATTGTAGCAAGAGAGGCCCTTCGGTCAGAACCACTACTGACAGTGGAAAGTTGAT- CACTGAC TGGTGCTGTCGCAGTTGCTCCCTTCCGCCCCTACGATTCCGGACAGAAAATGGCTGCTGGTACGGAATGGAAAT- CAGACCTG TTATGCATGATGAAACAACACTCGTCAGATCACAGGTTCATGCTTTCAAAGGTGAAATGGTTGACCCTTTTCAG- CTGGGCCTT CTGGTGATGTTTCTGGCCACCCAGGAAGTCCTTCGCAAGAGGTGGACGGCCAGATTGACCATTCCTGCGGTTTT- GGGGGTC CTACTTGTGCTGATGCTTGGGGGTATCACTTACACTGATTTGGCGAGGTATGTGGTGCTAGTCGCTGCTGCTTT- CGCAGAGG CCAACAGTGGAGGAGACGTCCTGCACCTTGCTTTGATTGCTGTTTTTAAGATCCAACCAGCATTTTTAGTGATG- AACATGCTT AGCACGAGATGGACGAACCAAGAAAACGTGGTTCTGGTCCTAGGGGCTGCCTTTTTCCAATTGGCCTCAGTAGA- TCTGCAA ATAGGAGTCCACGGAATCCTGAATGCCGCCGCTATAGCATGGATGATTGTCCGAGCGATCACCTTCCCCACAAC- CTCCTCCG TCACCATGCCAGTCTTAGCGCTTCTAACTCCGGGGATGAGGGCTCTATACCTAGACACTTACAGAATCATCCTC- CTCGTCATA GGGATTTGCTCCCTGCTGCACGAGAGGAAAAAGACCATGGCGAAAAAGAAAGGAGCTGTACTCTTGGGCTTAGC- GCTCACA

TCCACTGGATGGTTCTCGCCCACCACTATAGCTGCCGGACTAATGGTCTGCAACCCAAACAAGAAGAGAGGGTG- GCCAGCT ACTGAGTTTTTGTCGGCAGTTGGATTGATGTTTGCCATCGTAGGTGGTTTGGCCGAGTTGGATATTGAATCCAT- GTCAATACC CTTCATGCTGGCAGGTCTCATGGCAGTGTCCTACGTGGTGTCAGGAAAAGCAACAGATATGTGGCTTGAACGGG- CCGCCGA CATCAGCTGGGATATGGGTGCTGCAATCACAGGAAGCAGTCGGAGGCTGGATGTGAAACTGGATGATGACGGAG- ATTTTC ACTTGATTGATGATCCCGGTGTTCCATGGAAGGTCTGGGTCCTGCGCATGTCTTGCATTGGCTTAGCCGCCCTC- ACGCCTTGG GCCATCGTTCCCGCCGCTTTCGGTTATTGGCTCACTTTAAAAACAACAAAAAGAGGGGGCGTGTTTTGGGACAC- GCCATCCC CAAAACCTTGCTCAAAAGGAGACACCACTACAGGAGTCTACCGAATTATGGCTAGAGGGATTCTTGGCACTTAC- CAGGCCG GCGTCGGAGTCATGTACGAGAATGTTTTCCACACACTATGGCACACAACTAGAGGAGCAGCCATTGTGAGTGGA- GAAGGAA AATTGACGCCATACTGGGGTAGTGTGAAAGAAGACCGCATAGCTTACGGAGGCCCATGGAGGTTTGACCGAAAA- TGGAAT GGAACAGATGACGTGCAAGTGATCGTGGTAGAACCGGGGAAGGGCGCAGTAAACATCCAGACAAAACCAGGAGT- GTTTCG GACTCCCTTCGGGGAGGTTGGGGCTGTTAGTCTGGATTACCCGCGAGGAACATCCGGCTCACCCATTCTGGATT- CCAATGGA GACATTATAGGCCTATACGGCAATGGAGTTGAGCTTGGCGATGGCTCATACGTCAGCGCCATCGTGCAGGGTGA- CCGTCAG GAGGAACCAGTCCCAGAAGCTTACACCCCAAACATGTTGAGAAAGAGACAGATGACTGTGCTAGATTTGCACCC- TGGTTCA GGGAAAACCAGGAAAATTCTGCCACAAATAATTAAGGACGCTATCCAGCAGCGCCTAAGAACAGCTGTGTTGGC- ACCGACG CGGGTGGTAGCAGCAGAAATGGCAGAAGCTTTGAGAGGGCTCCCAGTACGATATCAAACTTCAGCAGTGCAGAG- AGAGCA CCAAGGGAATGAAATAGTGGATGTGATGTGCCACGCCACTCTGACCCATAGACTGATGTCACCGAACAGAGTGC- CCAACTA CAACCTATTTGTCATGGATGAAGCTCATTTCACCGACCCAGCCAGTATAGCCGCACGAGGATACATTGCTACCA- AGGTGGAA TTAGGGGAGGCAGCAGCCATCTTTATGACAGCGACCCCGCCTGGAACCACGGATCCTTTTCCTGACTCAAATGC- CCCAATCC ATGATTTGCAAGATGAGATACCAGACAGGGCATGGAGCAGTGGATACGAATGGATCACAGAATATGCGGGTAAA- ACCGTG TGGTTTGTGGCGAGCGTAAAAATGGGGAATGAGATTGCAATGTGCCTCCAAAGAGCGGGGAAAAAGGTCATCCA- ACTCAA CCGCAAGTCCTATGACACAGAATACCCAAAATGTAAGAATGGAGACTGGGATTTTGTCATTACCACCGACATCT- CTGAAATG GGGGCCAACTTCGGTGCGAGCAGGGTCATCGACTGTAGAAAGAGCGTGAAACCCACCATCTTAGAAGAGGGAGA- AGGCA GAGTCATCCTCGGAAACCCATCTCCCATAACCAGTGCAAGCGCAGCTCAACGGAGGGGCAGAGTAGGCAGAAAC- CCCAATC AAGTTGGAGATGAATACCACTATGGGGGGGCTACCAGTGAAGATGACAGTAACCTAGCCCATTGGACAGAGGCA- AAGATC ATGTTAGACAACATACACATGCCCAATGGACTGGTGGCCCAGCTCTATGGACCAGAGAGGGAAAAGGCTTTCAC- AATGGAT GGCGAATACCGTCTCAGAGGTGAAGAAAAGAAAAACTTCTTAGAGCTGCTTAGGACGGCTGACCTCCCGGTGTG- GCTGGCC TACAAGGTGGCGTCCAATGGCATTCAGTACACCGACAGAAAGTGGTGTTTTGATGGGCCGCGTACGAATGCCAT- ACTGGAG GACAACACCGAGGTAGAGATAGTCACCCGGATGGGTGAGAGGAAAATCCTCAAGCCGAGATGGCTTGATGCAAG- AGTTTA TGCAGATCACCAGGCCCTCAAGTGGTTCAAAGACTTTGCAGCAGGGAAGAGATCAGCCGTTAGCTTCATAGAGG- TGCTCGG TCGCATGCCTGAGCATTTCATGGGAAAGACGCGGGAAGCTTTAGACACCATGTACTTGGTTGCAACGGCTGAGA- AAGGTGG GAAAGCACACCGAATGGCTCTCGAAGAGCTGCCAGATGCACTGGAAACCATCACACTTATTGTCGCCATTACTG- TGATGACA GGAGGATTCTTCCTACTAATGATGCAGCGAAAGGGTATAGGGAAGATGGGTCTTGGAGCTCTAGTGCTCACACT- AGCTACC TTCTTCCTGTGGGCGGCAGAGGTTCCTGGAACCAAAATAGCAGGGACCCTGCTGATCGCCCTGCTGCTGATGGT- GGTTCTCA TCCCAGAACCGGAAAAACAGAGGTCACAGACAGATAACCAACTGGCGGTGTTTCTCATCTGTGTCTTGACCGTG- GTTGGAG TGGTGGCAGCAAACGAGTACGGGATGCTAGAAAAAACCAAAGCGGATCTCAAGAGCATGTTTGGCGGAAAGACG- CAGGCA TCAGGACTGACTGGATTGCCAAGCATGGCACTGGACCTGCGTCCAGCCACAGCCTGGGCACTGTATGGGGGGAG- CACAGTC GTGCTAACCCCTCTTCTGAAGCACCTGATCACGTCGGAATACGTCACCACATCGCTAGCTTCAATTAACTCACA- AGCTGGCTC ATTATTCGTCTTGCCACGAGGCGTGCCTTTTACCGACCTAGACTTGACTGTTGGCCTCGTCTTCCTTGGCTGTT- GGGGTCAAG TCACCCTCACAACGTTTCTGACAGCCATGGTTCTGGCGACACTTCACTATGGGTACATGCTCCCTGGATGGCAA- GCAGAAGC ACTCAGGGCTGCCCAGAGAAGGACAGCGGCTGGAATAATGAAGAATGCCGTTGTTGACGGAATGGTCGCCACTG- ATGTGC CTGAACTGGAAAGGACTACTCCTCTGATGCAAAAGAAAGTCGGACAGGTGCTCCTCATAGGGGTAAGCGTGGCA- GCGTTCC TCGTCAACCCTAATGTCACCACTGTGAGAGAAGCAGGGGTGTTGGTGACGGCGGCTACGCTTACTTTGTGGGAC- AATGGAG CCAGTGCCGTTTGGAATTCCACCACAGCCACGGGACTCTGCCATGTCATGCGAGGTAGCTACCTGGCTGGAGGC- TCCATTGC TTGGACTCTCATCAAGAACGCTGATAAGCCCTCCTTGAAAAGGGGAAGGCCTGGGGGCAGGACGCTAGGGGAGC- AGTGGA AGGAAAAACTAAATGCCATGAGTAGAGAAGAGTTTTTTAAATACCGGAGAGAGGCCATAATCGAGGTGGACCGC- ACTGAA GCACGCAGGGCCAGACGTGAAAATAACATAGTGGGAGGACATCCGGTTTCGCGAGGCTCAGCAAAACTCCGTTG- GCTCGT GGAGAAAGGATTTGTCTCGCCAATAGGAAAAGTCATTGATCTAGGGTGTGGGCGTGGAGGATGGAGCTACTACG- CAGCAA CCCTGAAGAAGGTCCAGGAAGTCAGAGGATACACGAAAGGTGGGGCGGGACATGAAGAACCGATGCTCATGCAG- AGCTA CGGCTGGAACCTGGTCTCCCTGAAGAGTGGAGTGGACGTGTTTTACAAACCTTCAGAGCCCAGTGATACCCTGT- TCTGTGAC ATAGGGGAATCCTCCCCAAGTCCAGAAGTAGAAGAACAACGCACACTACGCGTCCTAGAGATGACATCTGACTG- GTTGCAC CGAGGACCTAGAGAGTTCTGCATTAAAGTTCTCTGCCCTTACATGCCCAAGGTTATAGAAAAAATGGAAGTTCT- GCAGCGTC GCTTCGGAGGTGGGCTAGTGCGTCTCCCCCTGTCCCGAAACTCCAATCACGAGATGTATTGGGTTAGTGGAGCC- GCTGGCA ATGTGGTGCACGCTGTGAACATGACCAGCCAGGTATTACTGGGGCGAATGGATCGCACAGTGTGGAGAGGGCCA- AAGTAT GAGGAAGATGTCAACCTAGGGAGCGGAACAAGAGCCGTGGGAAAGGGAGAAGTCCATAGCAATCAGGAGAAAAT- CAAGA AGAGAATCCAGAAGCTTAAAGAAGAATTCGCCACAACGTGGCACAAAGACCCTGAGCATCCATACCGCACTTGG- ACATACC ACGGAAGCTATGAAGTGAAGGCTACTGGCTCAGCCAGCTCTCTCGTCAACGGAGTGGTGAAGCTCATGAGCAAA- CCTTGGG ACGCCATTGCCAACGTCACCACCATGGCCATGACTGACACCACCCCTTTTGGACAGCAAAGAGTTTTCAAGGAG- AAAGTTGA CACGAAGGCTCCTGAGCCACCAGCTGGAGCCAAGGAAGTGCTCAACGAGACCACCAACTGGCTGTGGGCCTACT- TGTCACG GGAAAAAAGACCCCGCTTGTGCACCAAGGAAGAATTCATTAAGAAAGTTAACAGCAACGCGGCTCTTGGAGCAG- TGTTCGC TGAACAGAATCAATGGAGCACGGCGCGTGAGGCTGTGGATGACCCGCGGTTTTGGGAGATGGTTGATGAAGAGA- GGGAA AACCATCTGCGAGGAGAGTGTCACACATGTATCTACAACATGATGGGAAAAAGAGAGAAGAAGCCTGGAGAGTT- TGGAAA AGCTAAAGGAAGCAGGGCCATTTGGTTCATGTGGCTTGGAGCACGGTATCTAGAGTTTGAAGCTTTGGGGTTCC- TGAATGA AGACCATTGGCTGAGCCGAGAGAATTCAGGAGGTGGAGTGGAAGGCTCAGGCGTCCAAAAGCTGGGATACATCC- TCCGTG ACATAGCAGGAAAGCAAGGAGGGAAAATGTACGCTGATGATACCGCCGGGTGGGACACTAGAATTACCAGAACT- GATTTA GAAAATGAAGCTAAGGTACTGGAGCTCCTAGACGGTGAACACCGCATGCTCGCCCGAGCCATAATTGAACTGAC- TTACAGG CACAAAGTGGTCAAGGTCATGAGACCTGCAGCAGAAGGAAAGACCGTGATGGACGTGATATCAAGAGAAGATCA- AAGGG GGAGTGGACAGGTGGTCACTTATGCTCTTAACACTTTCACGAACATCGCTGTCCAGCTCGTCAGGCTGATGGAG- GCTGAGG GGGTCATTGGACCACAACACTTGGAACATCTACCTAGGAAAAACAAGATAGCTGTCAGGACCTGGCTCTTTGAG- AATGGAG AGGAGAGAGTGACCAGGATGGCGATCAGCGGAGACGACTGTGCCGTCAAACCGCTGGACGACAGATTCGCCACA- GCCCTC CACTTCCTCAACGCAATGTCAAAGGTCAGAAAAGACATCCAGGAATGGAAGCCTTCGCATGGCTGGCACGATTG- GCAGCAA GTTCCCTTCTGTTCTAACCATTTTCAGGAGATTGTGATGAAAGATGGAAGGAGTATAGTTGTCCCGTGCAGAGG- ACAGGATG AGCTGATAGGCAGGGCTCGCATCTCTCCTGGAGCTGGATGGAATGTGAAGGACACAGCTTGCCTGGCCAAAGCA- TATGCAC AGATGTGGCTACTCCTATACTTCCATCGCAGGGACTTGCGTCTCATGGCAAATGCGATTTGCTCAGCAGTGCCA- GTAGATTG GGTGCCCACAGGCAGGACATCCTGGTCAATACACTCGAAAGGAGAGTGGATGACCACGGAAGACATGCTGCAGG- TCTGGA ACAGAGTTTGGATTGAAGAAAATGAATGGATGATGGACAAGACTCCAATCACAAGCTGGACAGACGTTCCGTAT- GTGGGA AAGCGCGAGGACATCTGGTGTGGCAGCCTCATCGGAACGCGATCCAGAGCAACCTGGGCTGAGAACATCTATGC- GGCGAT AAACCAGGTTAGAGCTGTCATTGGGAAAGAAAATTATGTTGACTACATGACCTCACTCAGGAGATACGAAGACG- TCTTGATC CAGGAAGACAGGGTCATCTAGTGTGATTTAAGGTAGAAAAGTAGACTATGTAAACAATGTAAATGAGAAAATGC- ATGCATA TGGAGTCAGGCCAGCAAAAGCTGCCACCGGATACTGGGTAGACGGTGCTGCCTGCGTCTCAGTCCCAGGAGGAC- TGGGTT AACAAATCTGACAACAGAAAGTGAGAAAGCCCTCAGAACCGTCTCGGAAGTAGGTCCCTGCTCACTGGAAGTTG- AAAGACC AACGTCAGGCCACAAATTTGTGCCACTCCGCTAGGGAGTGCGGCCTGCGCAGCCCCAGGAGGACTGGGTTACCA- AAGCCGT TGAGGCCCCCACGGCCCAAGCCTCGTCTAGGATGCAATAGACGAGGTGTAAGGACTAGAGGTTAGAGGAGACCC- CGTGGA AACAACAACATGCGGCCCAAGCCCCCTCGAAGCTGTAGAGGAGGTGGAAGGACTAGAGGTTAGAGGAGACCCCG- CATTTG CATCAAACAGCATATTGACACCTGGGAATAGACTGGGAGATCTTCTGCTCTATCTCAACATCAGCTACTAGGCA- CAGAGCGC

CGAAGTATGTAGCTGGTGGTGAGGAAGAACACAGGATCT Japanese encephalitis virus strain SA14-14-2, complete genome, ACCESSION: AF315119 SEQ ID NO: 75 AGAAGTTTATCTGTGTGAACTTCTTGGCTTAGTATCGTAGAGAAGAATCGAGAGATTAGTGCAGTTTAAACAGT- TTTTTAGA ACGGAAGATAACCATGACTAAAAAACCAGGAGGGCCCGGTAAAAACCGGGCTATCAATATGCTGAAACGCGGCC- TACCCC GCGTATTCCCACTAGTGGGAGTGAAGAGGGTAGTAATGAGCTTGTTGGACGGCAGAGGGCCAGTACGTTTCGTG- CTGGCTC TTATCACGTTCTTCAAGTTTACAGCATTAGCCCCGACCAAGGCGCTTTCAGGCCGATGGAAAGCAGTGGAAAAG- AGTGTGG CAATGAAACATCTTACTAGTTTCAAACGAGAACTTGGAACACTCATTGACGCCGTGAACAAGCGGGGCAGAAAG- CAAAACA AAAGAGGAGGAAATGAAGGCTCAATCATGTGGCTCGCGAGCTTGGCAGTTGTCATAGCTTGTGCAGGAGCCATG- AAGTTGT CGAATTTCCAGGGGAAGCTTTTGATGACCATCAACAACACGGACATTGCAGACGTTATCGTGATTCCCACCTCA- AAAGGAGA GAACAGATGCTGGGTCCGGGCAATCGACGTCGGCTACATGTGTGAGGACACTATCACGTACGAATGTCCTAAGC- TTACCAT GGGCAATGATCCAGAGGATGTGGATTGCTGGTGTGACAACCAAGAAGTCTACGTCCAATATGGACGGTGCACGC- GGACCA GGCATTCCAAGCGAAGCAGGAGATCCGTGTCGGTCCAAACACATGGGGAGAGTTCACTAGTGAATAAAAAAGAG- GCTTGG CTGGATTCAACGAAAGCCACACGATATCTCATGAAAACTGAGAACTGGATCATAAGGAATCCTGGCTATGCTTT- CCTGGCGG CGGTACTTGGCTGGATGCTTGGCAGTAACAACGGTCAACGCGTGGTATTTACCATCCTCCTGCTGTTGGTCGCT- CCGGCTTA CAGTTTTAATTGTCTGGGAATGGGCAATCGTGACTTCATAGAAGGAGCCAGTGGAGCCACTTGGGTGGACTTGG- TGCTAGA AGGAGACAGCTGCTTGACAATCATGGCAAACGACAAACCAACATTGGACGTCCGCATGATTAACATCGAAGCTA- GCCAACT TGCTGAGGTCAGAAGTTACTGCTATCATGCTTCAGTCACTGACATCTCGACGGTGGCTCGGTGCCCCACGACTG- GAGAAGCC CACAACGAGAAGCGAGCTGATAGTAGCTATGTGTGCAAACAAGGCTTCACTGACCGTGGGTGGGGCAACGGATG- TGGATT TTTCGGGAAGGGAAGCATTGACACATGTGCAAAATTCTCCTGCACCAGTAAAGCGATTGGGAGAACAATCCAGC- CAGAAAA CATCAAATACAAAGTTGGCATTTTTGTGCATGGAACCACCACTTCGGAAAACCATGGGAATTATTCAGCGCAAG- TTGGGGCG TCCCAGGCGGCAAAGTTTACAGTAACACCCAATGCTCCTTCGGTAGCCCTCAAACTTGGTGACTACGGAGAAGT- CACACTGG ACTGTGAGCCAAGGAGTGGACTGAACACTGAAGCGTTTTACGTCATGACCGTGGGGTCAAAGTCATTTCTGGTC- CATAGGG AGTGGTTTCATGACCTCGCTCTCCCCTGGACGTCCCCTTCGAGCACAGCGTGGAGAAACAGAGAACTCCTCATG- GAATTTGA AGGGGCGCACGCCACAAAACAGTCCGTTGTTGCTCTTGGGTCACAGGAAGGAGGCCTCCATCATGCGTTGGCAG- GAGCCAT CGTGGTGGAGTACTCAAGCTCAGTGATGTTAACATCAGGCCACCTGAAATGTAGGCTGAAAATGGACAAACTGG- CTCTGAA AGGCACAACCTATGGCATGTGTACAGAAAAATTCTCGTTCGCGAAAAATCCGGTGGACACTGGTCACGGAACAG- TTGTCATT GAACTCTCCTACTCTGGGAGTGATGGCCCCTGCAAAATTCCGATTGTTTCCGTTGCGAGCCTCAATGACATGAC- CCCCGTTGG GCGGCTGGTGACAGTGAACCCCTTCGTCGCGACTTCCAGTGCCAACTCAAAGGTGCTGGTCGAGATGGAACCCC- CCTTCGG AGACTCCTACATCGTAGTTGGAAGGGGAGACAAGCAGATCAACCACCATTGGCACAAAGCTGGAAGCACGCTGG- GCAAGG CCTTTTCAACAACTTTGAAGGGAGCTCAAAGACTGGCAGCGTTGGGCGACACAGCCTGGGACTTTGGCTCTATT- GGAGGGG TCTTCAACTCCATAGGAAGAGCCGTTCACCAAGTGTTTGGTGATGCCTTCAGAACACTCTTTGGGGGAATGTCT- TGGATCAC ACAAGGGCTAATGGGTGCCCTACTGCTCTGGATGGGCGTCAACGCACGAGACCGATCAATTGCTTTGGCCTTCT- TAGCCACA GGAGGTGTGCTCGTGTTCTTAGCGACCAATGTGCATGCTGACACTGGATGTGCCATTGACATCACAAGAAAAGA- GATGAGA TGTGGAAGTGGCATCTTCGTGCACAACGACGTGGAAGCCTGGGTGGATAGGTATAAATATTTGCCAGAAACGCC- CAGATCC CTAGCGAAGATCGTCCACAAAGCGCACAAGGAAGGCGTGTGCGGAGTCAGATCTGTCACTAGACTGGAGCACCA- AATGTG GGAAGCCGTAAGGGACGAATTGAACGTCCTGCTCAAAGAGAATGCAGTGGACCTCAGTGTGGTTGTGAACAAGC- CCGTGG GAAGATATCGCTCAGCCCCTAAACGCCTATCCATGACGCAAGAGAAGTTTGAAATGGGCTGGAAAGCATGGGGA- AAAAGC ATCCTCTTTGCCCCGGAATTGGCTAACTCCACATTTGTCGTAGATGGACCTGAGACAAAGGAATGCCCTGATGA- GCACAGAG CTTGGAACAGCATGCAAATCGAAGACTTCGGCTTTGGCATCACATCAACCCGTGTGTGGCTGAAAATTAGAGAG- GAGAGCA CTGACGAGTGTGATGGAGCGATCATAGGCACGGCTGTCAAAGGACATGTGGCAGTCCATAGTGACTTGTCGTAC- TGGATTG AGAGTCGCTACAACGACACATGGAAACTTGAGAGGGCAGTCTTTGGAGAGGTCAAATCTTGCACTTGGCCAGAG- ACACACA CCCTTTGGGGAGATGATGTTGAGGAAAGTGAACTCATCATTCCGCACACCATAGCCGGACCAAAAAGCAAGCAC- AATCGGA GGGAAGGGTATAAGACACAAAACCAGGGACCTTGGGATGAGAATGGCATAGTCTTGGACTTTGATTATTGCCCA- GGGACA AAAGTCACCATTACAGAGGATTGTAGCAAGAGAGGCCCTTCGGTCAGAACCACTACTGACAGTGGAAAGTTGAT- CACTGAC TGGTGCTGTCGCAGTTGCTCCCTTCCGCCCCTACGATTCCGGACAGAAAATGGCTGCTGGTACGGAATGGAAAT- CAGACCTG TTATGCATGATGAAACAACACTCGTCAGATCACAGGTTCATGCTTTCAAAGGTGAAATGGTTGACCCTTTTCAG- CTGGGCCTT CTGGTGATGTTTCTGGCCACCCAGGAAGTCCTTCGCAAGAGGTGGACGGCCAGATTGACCATTCCTGCGGTTTT- GGGGGTC CTACTTGTGCTGATGCTTGGGGGTATCACTTACACTGATTTGGCGAGGTATGTGGTGCTAGTCGCTGCTGCTTT- CGCAGAGG CCAACAGTGGAGGAGACGTCCTGCACCTTGCTTTGATTGCTGTTTTTAAGATCCAACCAGCATTTTTAGTGATG- AACATGCTT AGCACGAGATGGACGAACCAAGAAAACGTGGTTCTGGTCCTAGGGGCTGCCTTTTTCCAATTGGCCTCAGTAGA- TCTGCAA ATAGGAGTCCACGGAATCCTGAATGCCGCCGCTATAGCATGGATGATTGTCCGAGCGATCACCTTCCCCACAAC- CTCCTCCG TCACCATGCCAGTCTTAGCGCTTCTAACTCCGGGGATGAGGGCTCTATACCTAGACACTTACAGAATCATCCTC- CTCGTCATA GGGATTTGCTCCCTGCTGCACGAGAGGAAAAAGACCATGGCGAAAAAGAAAGGAGCTGTACTCTTGGGCTTAGC- GCTCACA TCCACTGGATGGTTCTCGCCCACCACTATAGCTGCCGGACTAATGGTCTGCAACCCAAACAAGAAGAGAGGGTG- GCCAGCT ACTGAGTTTTTGTCGGCAGTTGGATTGATGTTTGCCATCGTAGGTGGTTTGGCCGAGTTGGATATTGAATCCAT- GTCAATACC CTTCATGCTGGCAGGTCTCATGGCAGTGTCCTACGTGGTGTCAGGAAAAGCAACAGATATGTGGCTTGAACGGG- CCGCCGA CATCAGCTGGGATATGGGTGCTGCAATCACAGGAAGCAGTCGGAGGCTGGATGTGAAACTGGATGATGACGGAG- ATTTTC ACTTCATTGATGATCCCGGTGTTCCATGGAAGGTCTGGGTCCTGCGCATGTCTTGCATTGGCTTAGCCGCCCTC- ACGCCTTGG GCCATCGTTCCCGCCGCTTTCGGTTATTGGCTCACTTTAAAAACAACAAAAAGAGGGGGCGTGTTTTGGGACAC- GCCATCCC CAAAACCTTGCTCAAAAGGAGACACCACTACAGGAGTCTACCGAATTATGGCTAGAGGGATTCTTGGCACTTAC- CAGGCCG GCGTCGGAGTCATGTACGAGAATGTTTTCCACACACTATGGCACACAACTAGAGGAGCAGCCATTGTGAGTGGA- GAAGGAA AATTGACGCCATACTGGGGTAGTGTGAAAGAAGACCGCATAGCTTACGGAGGCCCATGGAGGTTTGACCGAAAA- TGGAAT GGAACAGATGACGTGCAAGTGATCGTGGTAGAACCGGGGAAGGGCGCAGTAAACATCCAGACAAAACCAGGAGT- GTTTCG GACTCCCTTCGGGGAGGTTGGGGCTGTTAGTCTGGATTACCCGCGAGGAACATCCGGCTCACCCATTCTGGATT- CCAATGGA GACATTATAGGCCTATACGGCAATGGAGTTGAGCTTGGCGATGGCTCATACGTCAGCGCCATCGTGCAGGGTGA- CCGTCAG GAGGAACCAGTCCCAGAAGCTTACACCCCAAACATGTTGAGAAAGAGACAGATGACTGTGCTAGATTTGCACCC- TGGTTCA GGGAAAACCAGGAAAATTCTGCCACAAATAATTAAGGACGCTATCCAGCAGCGCCTAAGAACAGCTGTGTTGGC- ACCGACG CGGGTGGTAGCAGCAGAAATGGCAGAAGTTTTGAGAGGGCTCCCAGTACGATATCAAACTTCAGCAGTGCAGAG- AGAGCA CCAAGGGAATGAAATAGTGGATGTGATGTGCCACGCCACTCTGACCCATAGACTGATGTCACCGAACAGAGTGC- CCAACTA CAACCTATTTGTCATGGATGAAGCTCATTTCACCGACCCAGCCAGTATAGCCGCACGAGGATACATTGCTACCA- AGGTGGAA TTAGGGGAGGCAGCAGCCATCTTTATGACAGCGACCCCGCCTGGAACCACGGATCCTTTTCCTGACTCAAATGC- CCCAATCC ATGATTTGCAAGATGAGATACCAGACAGGGCATGGAGCAGTGGATACGAATGGATCACAGAATATGCGGGTAAA- ACCGTG TGGTTTGTGGCGAGCGTAAAAATGGGGAATGAGATTGCAATGTGCCTCCAAAGAGCGGGGAAAAAGGTCATCCA- ACTCAA CCGCAAGTCCTATGACACAGAATACCCAAAATGTAAGAATGGAGACTGGGATTTTGTCATTACCACCGACATCT- CTGAAATG GGGGCCAACTTCGGTGCGAGCAGGGTCATCGACTGTAGAAAGAGCGTGAAACCCACCATCTTAGAAGAGGGAGA- AGGCA GAGTCATCCTCGGAAACCCATCTCCCATAACCAGTGCAAGCGCAGCTCAACGGAGGGGCAGAGTAGGCAGAAAC- CCCAATC AAGTTGGAGATGAATACCACTATGGGGGGGCTACCAGTGAAGATGACAGTAACCTAGCCCATTGGACAGAGGCA- AAGATC ATGTTAGACAACATACACATGCCCAATGGACTGGTGGCCCAGCTCTATGGACCAGAGAGGGAAAAGGCTTTCAC- AATGGAT GGCGAATACCGTCTCAGAGGTGAAGAAAAGAAAAACTTCTTAGAGCTGCTTAGGACGGCTGACCTCCCGGTGTG- GCTGGCC TACAAGGTGGCGTCCAATGGCATTCAGTACACCGACAGAAAGTGGTGTTTTGATGGGCCGCGTACGAATGCCAT- ACTGGAG GACAACACCGAGGTAGAGATAGTCACCCGGATGGGTGAGAGGAAAATCCTCAAGCCGAGATGGCTTGATGCAAG- AGTTTA TGCAGATCACCAGGCCCTCAAGTGGTTCAAAGACTTTGCAGCAGGGAAGAGATCAGCCGTTAGCTTCATAGAGG- TGCTCGG TCGCATGCCTGAGCATTTCATGGGAAAGACGCGGGAAGCTTTAGACACCATGTACTTGGTTGCAACGGCTGAGA- AAGGTGG GAAAGCACACCGAATGGCTCTCGAAGAGCTGCCAGATGCACTGGAAACCATCACACTTATTGTCGCCATTACTG- TGATGACA

GGAGGATTCTTCCTACTAATGATGCAGCGAAAGGGTATAGGGAAGATGGGTCTTGGAGCTCTAGTGCTCACACT- AGCTACC TTCTTCCTGTGGGCGGCAGAGGTTCCTGGAACCAAAATAGCAGGGACCCTGCTGATCGCCCTGCTGCTGATGGT- GGTTCTCA TCCCAGAACCGGAAAAACAGAGGTCACAGACAGATAACCAACTGGCGGTGTTTCTCATCTGTGTCTTGACCGTG- GTTGGAG TGGTGGCAGCAAACGAGTACGGGATGCTAGAAAAAACCAAAGCGGATCTCAAGAGCATGTTTGGCGGAAAGACG- CAGGCA TCAGGACTGACTGGATTGCCAAGCATGGCACTGGACCTGCGTCCAGCCACAGCCTGGGCACTGTATGGGGGGAG- CACAGTC GTGCTAACCCCTCTTCTGAAGCACCTGATCACGTCGGAATACGTCACCACATCGCTAGCTTCAATTAACTCACA- AGCTGGCTC ATTATTCGTCTTGCCACGAGGCGTGCCTTTTACCGACCTAGACTTGACTGTTGGCCTCGTCTTCCTTGGCTGTT- GGGGTCAAG TCACCCTCACAACGTTTCTGACAGCCATGGTTCTGGCGACACTTCACTATGGGTACATGCTCCCTGGATGGCAA- GCAGAAGC ACTCAGGGCTGCCCAGAGAAGGACAGCGGCTGGAATAATGAAGAATGCCGTTGTTGACGGAATGGTCGCCACTG- ATGTGC CTGAACTGGAAAGGACTACTCCTCTGATGCAAAAGAAAGTCGGACAGGTGCTCCTCATAGGGGTAAGCGTGGCA- GCGTTCC TCGTCAACCCTAATGTCACCACTGTGAGAGAAGCAGGGGTGTTGGTGACGGCGGCTACGCTTACTTTGTGGGAC- AATGGAG CCAGTGCCGTTTGGAATTCCACCACAGCCACGGGACTCTGCCATGTCATGCGAGGTAGCTACCTGGCTGGAGGC- TCCATTGC TTGGACTCTCATCAAGAACGCTGATAAGCCCTCCTTGAAAAGGGGAAGGCCTGGGGGCAGGACGCTAGGGGAGC- AGTGGA AGGAAAAACTAAATGCCATGAGTAGAGAAGAGTTTTTTAAATACCGGAGAGAGGGCATAATCGAGGTGGACCGC- ACTGAA GCACGCAGGGCCAGAAGTGAAAATAACATAGTGGGAGGACATCCGGTTTCGCGAGGCTCAGCAAAACTCCGTTG- GCTTGT GGAGAAAGGATTTGTCTCGCCAATAGGAAAAGTCATTGATCTAGGGTGTGGGCGTGGAGGATGGAGCTACTACG- CAGCAA CCCTGAAGAAGGTCCAGGAAGTCAGAGGATACACGAAAGGTGGGGCGGGACATGAAGAACCGATGCTCATGCAG- AGCTA CGGCTGGAACCTGGTCTCCCTGAAGAGTGGAGTGGACGTGTTTTACAAACCTTCAGAGCCCAGTGATACCCTGT- TCTGTGAC ATAGGGGAATCCTCCCCAAGTCCAGAAGTAGAAGAACAACGCACACTACGCGTCCTAGAGATGACATCTGACTG- GTTGCAC CGAGGACCTAGAGAGTTCTGCATTAAAGTTCTCTGCCCTTACATGCCCAAGGTTATAGAAAAAATTGAAGTTCT- GCAGCGCC GCTTCGGAGGTGGGCTAGTGCGTCTCCCCCTGTCCCGAAACTCCAATCACGAGATGTATTGGGTTAGTGGAGCC- GCTGGCA ATGTGGTGCACGCTGTGAACATGACCAGCCAGGTATTACTGGGGCGAATGGATCGCACAGTGTGGAGAGGGCCA- AAGTAT GAGGAAGATGTCAACCTAGGGAGCGGAACAAGAGCCGTGGGAAAGGGAGAAGTCCATAGCAATCAGGAGAAAAT- CAAGA AGAGAATCCAGAAGCTTAAAGAAGAATTCGCCACAACGTGGCACAAAGACCCTGAGCATCCATACCGCACTTGG- ACATACC ACGGAAGCTATGAAGTGAAGGCTACTGGCTCAGCCAGCTCTCTCGTCAACGGAGTGGTGAAGCTCATGAGCAAA- CCTTGGG ACGCCATTGCCAACGTCACCACCATGGCCATGACTGACACCACCCCTTTTGGACAGCAAAGAGTTTTCAAGGAG- AAAGTTGA CACGAAGGCTCCTGAGCCACCAGCTGGAGCCAAGGAAGTGCTCAACGAGACCACCAACTGGCTGTGGGCCTACT- TGTCACG GGAAAAAAGACCCCGCTTGTGCACCAAGGAAGAATTCATTAAGAAAGTTAACAGCAACGCGGCTCTTGGAGCAG- TGTTCGC TGAACAGAATCAATGGAGCACGGCGCGTGAGGCTGTGGATGACCCGCGGTTTTGGGAGATGGTTGATGAAGAGA- GGGAA AACCATCTGCGAGGAGAGTGTCACACATGTATCTACAACATGATGGGAAAAAGAGAGAAGAAGCCTGGAGAGTT- TGGAAA AGCTAAAGGAAGCAGGGCCATTTGGTTCATGTGGCTTGGAGCACGGTATCTAGAGTTTGAAGCTTTGGGGTTCC- TGAATGA AGACCATTGGCTGAGCCGAGAGAATTCAGGAGGTGGAGTGGAAGGCTCAGGCGTCCAAAAGCTGGGATACATCC- TCCGTG ACATAGCAGGAAAGCAAGGAGGGAAAATGTACGCTGATGATACCGCCGGGTGGGACACTAGAATTACCAGAACT- GATTTA GAAAATGAAGCTAAGGTACTGGAGCTCCTAGACGGTGAACACCGCATGCTCGCCCGAGCCATAATTGAACTGAC- TTACAGG CACAAAGTGGTCAAGGTCATGAGACCTGCAGCAGAAGGAAAGACCGTGATGGACGTGATATCAAGAGAAGATCA- AAGGG GGAGTGGACAGGTGGTCACTTATGCTCTTAACACTTTCACGAACATCGCTGTCCAGCTCGTCAGGCTGATGGAG- GCTGAGG GGGTCATTGGACCACAACACTTGGAACATCTACCTAGGAAAAACAAGATAGCTGTCAGGACCTGGCTCTTTGAG- AATGGAG AGGAGAGAGTGACCAGGATGGCGATCAGCGGAGACGACTGTGCCGTCAAACCGCTGGACGACAGATTCGCCACA- GCCCTC CACTTCCTCAACGCAATGTCAAAGGTCAGAAAAGACATCCAGGAATGGAAGCCTTCGCATGGCTGGCACGATTG- GCAGCAA GTTCCCTTCTGTTCTAACCATTTTCAGGAGATTGTGATGAAAGATGGAAGGAGTATAGTTGTCCCGTGCAGAGG- ACAGGATG AGCTGATAGGCAGGGCTCGCATCTCTCCAGGAGCTGGATGGAATGTGAAGGACACAGCTTGCCTGCCCAAAGCA- TATGCAC AAATGTGGGTACTCCTATACTTCCACCGCAGGGACTTGCGTCTCATGGCAAATGCGATTTGCTCAGCAGTGCCA- GTAGATTG GGTGCCCACAGGCAGGACATCCTGGTCAATACACTCGAAAGGAGAGTGGATGACCACGGAAGACATGCTGCAGG- TCTGGA ACAGAGTTTGGATTGAAGAAAATGAATGGATGATGGACAAGACTCCAATCACAAGCTGGACAGACGTTCCGTAT- GTGGGA AAGCGCGAGGACATCTGGTGTGGCAGCCTCATCGGAACGCGATCCAGAGCAACCTGGGCTGAGAACATCTATGC- GGCGAT AAACCAGGTTAGAGCTGTCATTGGGAAAGAAAATTATGTTGACTACATGACCTCACTCAGGAGATACGAAGACG- TCTTGATC CAGGAAGACAGGGTCATCTAGTGTGATTTAAGGTAGAAAAGTAGACTATGTAAACAATGTAAATGAGAAAATGC- ATGCATA TGGAGTCAGGCCAGCAAAAGCTGCCACCGGATACTGGGTAGACGGTGCTGCCTGCGTCTCAGTCCCAGGAGGAC- TGGGTT AACAAATCTGACAACAGAAAGTGAGAAAGCCCTCAGAACTGTCTCGGAAGTAGGTCCCTGCTCACTGGAAGTTG- AAAGACC AACGTCAGGCCACAAATTTGTGCCACTCCGCTAGGGAGTGCGGCCTGCGCAGCCCCAGGAGGACTGGGTTACCA- AAGCCGT TGAGCCCCCACGGCCCAAGCCTCGTCTAGGATGCAATAGACGAGGTGTAAGGACTAGAGGTTAGAGGAGACCCC- GTGGAA ACAACAACATGCGGCCCAAGCCCCCTCGAAGCTGTAGAGGAGGTGGAAGGACTAGAGGTTAGAGGAGACCCCGC- ATTTGC ATCAAACAGCATATTGACACCTGGGAATAGACTGGGAGATCTTCTGCTCTATCTCAACATCAGCTACTAGGCAC- AGAGCGCC GAAGTATGTACGTGGTGGTGAGGAAGAACACAGGATCT >gi|564014614|gb|KF769015.1| Yellow fever virus strain 17D-204, complete genome SEQ ID NO: 76 GTGCTAATTGAGGTGCATTGGTCTGCAAATCGAGTTGCTAGGCAATAAACACATTTGGATTAATTTTAATCGTT- CGTTGAGCGA TTAGCAGAGAACTGACCAGAACATGTCTGGTCGTAAAGCTCAGGGAAAAACCCTGGGCGTCAATATGGTACGAC- GAGGAGTT CGCTCCTTGTCAAACAAAATAAAACAAAAAACAAAACAAATTGGAAACAGACCTGGACCTTCAAGAGGTGTTCA- AGGATTTAT CTTTTTCTTTTTGTTCAACATTTTGACTGGAAAAAAGATCACAGCCCACCTAAAGAGGTTGTGGAAAATGCTGG- ACCCAAGACA AGGCTTGGCTGTTCTAAGGAAAGTCAAGAGAGTGGTGGCCAGTTTGATGAGAGGATTGTCCTCAAGGAAACGCC- GTTCCCAT GATGTTCTGACTGTGCAATTCCTAATTTTGGGAATGCTGTTGATGACGGGTGGAGTGACCTTGGTGCGGAAAAA- CAGATGGTT GCTCCTAAATGTGACATCTGAGGACCTCGGGAAAACATTCTCTGTGGGCACAGGCAACTGCACAACAAACATTT- TGGAAGCCA AGTACTGGTGCCCAGACTCAATGGAATACAACTGTCCCAATCTCAGTCCAAGAGAGGAGCCAGATGACATTGAT- TGCTGGTGC TATGGGGTGGAAAACGTTAGAGTCGCATATGGTAAGTGTGACTCAGCAGGCAGGTCTAGGAGGTCAAGAAGGGC- CATTGACT TGCCTACGCATGAAAACCATGGTTTGAAGACCCGGCAAGAAAAATGGATGACTGGAAGAATGGGTGAAAGGCAA- CTCCAAAA GATTGAGAGATGGTTCGTGAGGAACCCCTTTTTTGCAGTGACGGCTCTGACCATTGCCTACCTTGTGGGAAGCA- ACATGACGC AACGAGTCGTGATTGCCCTACTGGTCTTGGCTGTTGGTCCGGCCTACTCAGCTCACTGCATTGGAATTACTGAC- AGGGATTTCA TTGAGGGGGTGCATGGAGGAACTTGGGTTTCAGCTACCCTGGAGCAAGACAAGTGTGTCACTGTTATGGCCCCT- GACAAGCCT TCATTGGACATCTCACTAGAGACAGTAGCCATTGATAGACCTGCTGAGGTGAGGAAAGTGTGTTACAATGCAGT- TCTCACTCAT GTGAAGATTAATGACAAGTGCCCCAGCACTGGAGAGGCCCACCTAGCTGAAGAGAACGAAGGGGACAATGCGTG- CAAGCGC ACTTATTCTGATAGAGGCTGGGGCAATGGCTGTGGCCTATTTGGGAAAGGGAGCATTGTGGCATGCGCCAAATT- CACTTGTGC CAAATCCATGAGTTTGTTTGAGGTTGATCAGACCAAAATTCAGTATGTCATCAGAGCACAATTGCATGTAGGGG- CCAAGCAGG AAAATTGGACTACCGACATTAAGACTCTCAAGTTTGATGCCCTGTCAGGCTCCCAGGAAGTCGAGTTCATTGGG- TATGGAAAA GCTACACTGGAATGCCAGGTGCAAACTGCGGTGGACTTTGGTAACAGTTACATCGCTGAGATGGAAACAGAGAG- CTGGATAG TGGACAGACAGTGGGCCCAGGACTTGACCCTGCCATGGCAGAGTGGAAGTGGCGGGGTGTGGAGAGAGATGCAT- CATCTTG TCGAATTTGAACCTCCGCATGCCGCCACTATCAGAGTACTGGCCCTGGGAAACCAGGAAGGCTCCTTGAAAACA- GCTCTTACT GGCGCAATGAGGGTTACAAAGGACACAAATGACAACAACCTTTACAAACTACATGGTGGACATGTTTCTTGCAG- AGTGAAATT GTCAGCTTTGACACTCAAGGGGACATCCTACAAAATATGCACTGACAAAATGTTTTTTGTCAAGAACCCAACTG- ACACTGGCCA TGGCACTGTTGTGATGCAGGTGAAAGTGTCAAAAGGAGCCCCCTGCAGGATTCCAGTGATAGTAGCTGATGATC- TTACAGCG GCAATCAATAAAGGCATTTTGGTTACAGTTAACCCCATCGCCTCAACCAATGATGATGAAGTGCTGATTGAGGT- GAACCCACCT TTTGGAGACAGCTACATTATCGTTGGGAGAGGAGATTCACGTCTCACTTACCAGTGGCACAAAGAGGGAAGCTC- AATAGGAA AGTTGTTCACTCAGACCATGAAAGGCGTGGAACGCCTGGCCGTCATGGGAGACACCGCCTGGGATTTCAGCTCC- GCTGGAGG GTTCTTCACTTCGGTTGGGAAAGGAATTCATACGGTGTTTGGCTCTGCCTTTCAGGGGCTATTTGGCGGCTTGA- ACTGGATAAC AAAGGTCATCATGGGGGCGGTACTTATATGGGTTGGCATCAACACAAGAAACATGACAATGTCCATGAGCATGA- TCTTGGTAG

GAGTGATCATGATGTTTTTGTCTCTAGGAGTTGGGGCGGATCAAGGATGCGCCATCAACTTTGGCAAGAGAGAG- CTCAAGTGC GGAGATGGTATCTTCATATTTAGAGACTCTGATGACTGGCTGAACAAGTACTCATACTATCCAGAAGATCCTGT- GAAGCTTGCA TCAATAGTGAAAGCCTCTTTTGAAGAAGGGAAGTGTGGCCTAAATTCAGTTGACTCCCTTGAGCATGAGATGTG- GAGAAGCAG GGCAGATGAGATCAATGCCATTTTTGAGGAAAACGAGGTGGACATTTCTGTTGTCGTGCAGGATCCAAAGAATG- TTTACCAGA GAGGAACTCATCCATTTTCCAGAATTCGGGATGGTCTGCAGTATGGTTGGAAGACTTGGGGTAAGAACCTTGTG- TTCTCCCCA GGGAGGAAGAATGGAAGCTTCATCATAGATGGAAAGTCCAGGAAAGAATGCCCGTTTTCAAACCGGGTCTGGAA- TTCTTTCC AGATAGAGGAGTTTGGGACGGGAGTGTTCACCACACGCGTGTACATGGACGCAGTCTTTGAATACACCATAGAC- TGCGATGG ATCTATCTTGGGTGCAGCGGTGAACGGAAAAAAGAGTGCCCATGGCTCTCCAACATTTTGGATGGGAAGTCATG- AAGTAAAT GGGACATGGATGATCCACACCTTGGAGGCATTAGATTACAAGGAGTGTGAGTGGCCACTGACACATACGATTGG- AACATCAG TTGAAGAGAGTGAAATGTTCATGCCGAGATCAATCGGAGGCCCAGTTAGCTCTCACAATCATATCCCTGGATAC- AAGGTTCAG ACGAACGGACCTTGGATGCAGGTACCACTAGAAGTGAAGAGAGAAGCTTGCCCAGGGACTAGCGTGATCATTGA- TGGCAACT GTGATGGACGGGGAAAATCAACCAGATCCACCACGGATAGCGGGAAAGTTATTCCTGAATGGTGTTGCCGCTCC- TGCACAAT GCCGCCTGTGAGCTTCCATGGTAGTGATGGGTGTTGGTATCCCATGGAAATTAGGCCAAGGAAAACGCATGAAA- GCCATCTG GTGCGCTCCTGGGTTACAGCTGGAGAAATACATGCTGTCCCTTTTGGTTTGGTGAGCATGATGATAGCAATGGA- AGTGGTCCT AAGGAAAAGACAGGGACCAAAGCAAATGTTGGTTGGAGGAGTAGTGCTCTTGGGAGCAATGCTGGTCGGGCAAG- TAACTCT CCTTGATTTGCTGAAACTCACAGTGGCTGTGGGATTGCATTTCCATGAGATGAACAATGGAGGAGACGCCATGT- ATATGGCGT TGATTGCTGCCTTTTCAATCAGACCAGGGCTGCTCATCGGCTTTGGGCTCAGGACCCTATGGAGCCCTCGGGAA- CGCCTTGTGC TGACCCTAGGAGCAGCCATGGTGGAGATTGCCTTGGGTGGCGTGATGGGCGGCCTGTGGAAGTATCTAAATGCA- GTTTCTCTC TGCATCCTGACAATAAATGCTGTTGCTTCTAGGAAAGCATCAAATACCATCTTGCCCCTCATGGCTCTGTTGAC- ACCTGTCACTA TGGCTGAGGTGAGACTTGCCGCAATGTTCTTTTGTGCCGTGGTTATCATAGGGGTCCTTCACCAGAATTTCAAG- GACACCTCCA TGCAGAAGACTATACCTCTGGTGGCCCTCACACTCACATCTTACCTGGGCTTGACACAACCTTTTTTGGGCCTG- TGTGCATTTCT GGCAACCCGCATATTTGGGCGAAGGAGTATCCCAGTGAATGAGGCACTCGCAGCAGCTGGTCTAGTGGGAGTGC- TGGCAGG ACTGGCTTTTCAGGAGATGGAGAACTTCCTTGGTCCGATTGCAGTTGGAGGACTCCTGATGATGCTGGTTAGCG- TGGCTGGGA GGGTGGATGGGCTAGAGCTCAAGAAGCTTGGTGAAGTTTCATGGGAAGAGGAGGCGGAGATCAGCGGGAGTTCC- GCCCGCT ATGATGTGGCACTCAGTGAACAAGGGGAGTTCAAGCTGCTTTCTGAAGAGAAAGTGCCATGGGACCAGGTTGTG- ATGACCTC GCTGGCCTTGGTTGGGGCTGCCCTCCATCCATTTGCTCTTCTGCTGGTCCTTGCTGGGTGGCTGTTTCATGTCA- GGGGAGCTAG GAGAAGTGGGGATGTCTTGTGGGATATTCCCACTCCTAAGATCATCGAGGAATGTGAACATCTGGAGGATGGGA- TTTATGGC ATATTCCAGTCAACCTTCTTGGGGGCCTCCCAGCGAGGAGTGGGAGTGGCACAGGGAGGGGTGTTCCACACAAT- GTGGCATG TCACAAGAGGAGCTTTCCTTGTCAGGAATGGCAAGAAGTTGATTCCATCTTGGGCTTCAGTAAAGGAAGACCTT- GTCGCCTAT GGTGGCTCATGGAAGTTGGAAGGCAGATGGGATGGAGAGGAAGAGGTCCAGTTGATCGCGGCTGTTCCAGGAAA- GAACGTG GTCAACGTCCAGACAAAACCGAGCTTGTTCAAAGTGAGGAATGGGGGAGAAATCGGGGCTGTCGCTCTTGACTA- TCCGAGTG GCACTTCAGGATCTCCTATTGTTAACAGGAACGGAGAGGTGATTGGGCTGTACGGCAATGGCATCCTTGTCGGT- GACAACTCC TTCGTGTCCGCCATATCCCAGACTGAGGTGAAGGAAGAAGGAAAGGAGGAGCTCCAAGAGATCCCGACAATGCT- AAAGAAA GGAATGACAACTGTCCTTGATTTTCATCCTGGAGCTGGGAAGACAAGACGTTTCCTCCCACAGATCTTGGCCGA- GTGCGCACG GAGACGCTTGCGCACTCTTGTGTTGGCCCCCACCAGGGTTGTTCTTTCTGAAATGAAGGAGGCTTTTCACGGCC- TGGACGTGA AATTCCACACACAGGCTTTTTCCGCTCACGGCAGCGGGAGAGAAGTCATTGATGCTATGTGCCATGCCACCCTA- ACTTACAGGA TGTTGGAACCAACTAGGGTTGTTAACTGGGAAGTGATCATTATGGATGAAGCCCATTTTTTGGATCCAGCTAGC- ATAGCCGCTA GAGGTTGGGCAGCGCACAGAGCTAGGGCAAATGAAAGTGCAACAATCTTGATGACAGCCACACCGCCTGGGACT- AGTGATG AATTTCCACATTCAAATGGTGAAATAGAAGATGTTCAAACGGACATACCCAGTGAGCCCTGGAACACAGGGCAT- GACTGGATC CTGGCTGACAAAAGGCCCACGGCATGGTTCCTTCCATCCATCAGAGCTGCAAATGTCATGGCTGCCTCTTTGCG- TAAGGCTGG AAAGAGTGTGGTGGTCCTGAACAGGAAAACCTTTGAGAGAGAATACCCCACGATAAAGCAGAAGAAACCTGACT- TTATATTG GCCACTGACATAGCTGAAATGGGAGCCAACCTTTGCGTGGAGCGAGTGCTGGATTGCAGGACGGCTTTTAAGCC- TGTGCTTGT GGATGAAGGGAGGAAGGTGGCAATAAAAGGGCCACTTCGTATCTCCGCATCCTCTGCTGCTCAAAGGAGGGGGC- GCATTGG GAGAAATCCCAACAGAGATGGAGACTCATACTACTATTCTGAGCCTACAAGTGAAAATAATGCCCACCACGTCT- GCTGGTTGG AGGCCTCAATGCTCTTGGACAACATGGAGGTGAGGGGTGGAATGGTCGCCCCACTCTATGGCGTTGAAGGAACT- AAAACACC AGTTTCCCCTGGTGAAATGAGACTGAGGGATGACCAGAGGAAAGTCTTCAGAGAACTAGTGAGGAATTGTGACC- TGCCCGTT TGGCTTTCGTGGCAAGTGGCCAAGGCTGGTTTGAAGACGAATGATCGTAAGTGGTGTTTTGAAGGCCCTGAGGA- ACATGAGA TCTTGAATGACAGCGGTGAAACAGTGAAGTGCAGGGCTCCTGGAGGAGCAAAGAAGCCTCTGCGCCCAAGGTGG- TGTGATG AAAGGGTGTCATCTGACCAGAGTGCGCTGTCTGAATTTATTAAGTTTGCTGAAGGTAGGAGGGGAGCTGCTGAA- GTGCTAGTT GTGCTGAGTGAACTCCCTGATTTCCTGGCTAAAAAAGGTGGAGAGGCAATGGATACCATCAGTGTGTTTCTCCA- CTCTGAGGA AGGCTCTAGGGCTTACCGCAATGCACTATCAATGATGCCTGAGGCAATGACAATAGTCATGCTGTTTATACTGG- CTGGACTACT GACATCGGGAATGGTCATCTTTTTCATGTCTCCCAAAGGCATCAGTAGAATGTCTATGGCGATGGGCACAATGG- CCGGCTGTG GATATCTCATGTTCCTTGGAGGCGTCAAACCCACTCACATCTCCTATATCATGCTCATATTCTTTGTCCTGATG- GTGGTTGTGATC CCCGAGCCAGGGCAACAAAGGTCCATCCAAGACAACCAAGTGGCATACCTCATTATTGGCATCCTGACGCTGGT- TTCAGCGGT GGCAGCCAACGAGCTAGGCATGCTGGAGAAAACCAAAGAGGACCTCTTTGGGAAGAAGAACTTAATTCCATCTA- GTGCTTCAC CCTGGAGTTGGCCGGATCTTGACCTGAAGCCAGGAGCTGCCTGGACAGTGTACGTTGGCATTGTTACAATGCTC- TCTCCAATG TTGCACCACTGGATCAAAGTCGAATATGGCAACCTGTCTCTGTCTGGAATAGCCCAGTCAGCCTCAGTCCTTTC- TTTCATGGACA AGGGGATACCATTCATGAAGATGAATATCTCGGTCATAATGCTGCTGGTCAGTGGCTGGAATTCAATAACAGTG- ATGCCTCTG CTCTGTGGCATAGGGTGCGCCATGCTCCACTGGTCTCTCATTTTACCTGGAATCAAAGCGCAGCAGTCAAAGCT- TGCACAGAG AAGGGTGTTCCATGGCGTTGCCAAGAACCCTGTGGTTGATGGGAATCCAACAGTTGACATTGAGGAAGCTCCTG- AAATGCCTG CCCTTTATGAGAAGAAACTGGCTCTATATCTCCTTCTTGCTCTCAGCCTAGCTTCTGTTGCCATGTGCAGAACG- CCCTTTTCATTG GCTGAAGGCATTGTCCTAGCATCAGCTGCCCTAGGGCCGCTCATAGAGGGAAACACCAGCCTTCTTTGGAATGG- ACCCATGGC TGTCTCCATGACAGGAGTCATGAGGGGGAATCACTATGCTTTTGTGGGAGTCATGTACAATCTATGGAAGATGA- AAACTGGAC GCCGGGGGAGCGCGAATGGAAAAACTTTGGGTGAAGTCTGGAAGAGGGAACTGAATCTGTTGGACAAGCGACAG- TTTGAGT TGTATAAAAGGACCGACATTGTGGAGGTGGATCGTGATACGGCACGCAGGCATTTGGCCGAAGGGAAGGTGGAC- ACCGGGG TGGCGGTCTCCAGGGGGACCGCAAAGTTAAGGTGGTTCCATGAGCGTGGCTATGTCAAGCTGGAAGGTAGGGTG- ATTGACCT GGGGTGTGGCCGCGGAGGCTGGTGTTACTACGCTGCTGCGCAAAAGGAAGTGAGTGGGGTCAAAGGATTTACTC- TTGGAAG AGACGGCCATGAGAAACCCATGAATGTGCAAAGTCTGGGATGGAACATCATCACCTTCAAGGACAAAACTGATA- TCCACCGCC TAGAACCAGTGAAATGTGACACCCTTTTGTGTGACATTGGAGAGTCATCATCGTCATCGGTCACAGAGGGGGAA- AGGACCGT GAGAGTTCTTGATACTGTAGAAAAATGGCTGGCTTGTGGGGTTGACAACTTCTGTGTGAAGGTGTTAGCTCCAT- ACATGCCAG ATGTTCTCGAGAAACTGGAATTGCTCCAAAGGAGGTTTGGCGGAACAGTGATCAGGAACCCTCTCTCCAGGAAT- TCCACTCAT GAAATGTACTACGTGTCTGGAGCCCGCAGCAATGTCACATTTACTGTGAACCAAACATCCCGCCTCCTGATGAG- GAGAATGAG GCGTCCAACTGGAAAAGTGACCCTGGAGGCTGACGTCATCCTCCCAATTGGGACACGCAGTGTTGAGACAGACA- AGGGACCC CTGGACAAAGAGGCCATAGAAGAAAGGGTTGAGAGGATAAAATCTGAGTACATGACCTCTTGGTTTTATGACAA- TGACAACC CCTACAGGACCTGGCACTACTGTGGCTCCTATGTCACAAAAACCTCAGGAAGTGCGGCGAGCATGGTAAATGGT- GTTATTAAA ATTCTGACATATCCATGGGACAGGATAGAGGAGGTCACAAGAATGGCAATGACTGACACAACCCCTTTTGGACA- GCAAAGAG TGTTTAAAGAAAAAGTTGACACCAGAGCAAAGGATCCACCAGCGGGAACTAGGAAGATCATGAAAGTTGTCAAC- AGGTGGCT GTTCCGCCACCTGGCCAGAGAAAAGAACCCCAGACTGTGCACAAAGGAAGAATTTATTGCAAAAGTCCGAAGTC- ATGCAGCC ATTGGAGCTTACCTGGAAGAACAAGAACAGTGGAAGACTGCCAATGAGGCTGTCCAAGACCCAAAGTTCTGGGA- ACTGGTGG ATGAAGAAAGGAAGCTGCACCAACAAGGCAGGTGTCGGACTTGTGTGTACAACATGATGGGGAAAAGAGAGAAG- AAGCTGT CAGAGTTTGGGAAAGCAAAGGGAAGCCGTGCCATATGGTATATGTGGCTGGGAGCGCGGTATCTTGAGTTTGAG- GCCCTGGG ATTCCTGAATGAGGACCATTGGGCTTCCAGGGAAAACTCAGGAGGAGGAGTGGAAGGCATTGGCTTACAATACC- TAGGATAT GTGATCAGAGACCTGGCTGCAATGGATGGTGGTGGATTCTACGCGGATGACACCGCTGGATGGGACACGCGCAT- CACAGAG GCAGACCTTGATGATGAACAGGAGATCTTGAACTACATGAGCCCACATCACAAAAAACTGGCACAAGCAGTGAT-

GGAAATGA CATACAAGAACAAAGTGGTGAAAGTGTTGAGACCAGCCCCAGGAGGGAAAGCCTACATGGATGTCATAAGTCGA- CGAGACCA GAGAGGATCCGGGCAGGTAGTGACTTATGCTCTGAACACCATCACCAACTTGAAAGTCCAATTGATCAGAATGG- CAGAAGCA GAGATGGTGATACATCACCAACATGTTCAAGATTGTGATGAATCAGTTCTGACCAGGCTGGAGGCATGGCTCAC- TGAGCACGG ATGTAACAGACTGAAGAGGATGGCGGTGAGTGGAGACGACTGTGTGGTCCGGCCCATCGATGACAGGTTCGGCC- TGGCCCTG TCCCATCTCAACGCCATGTCCAAGGTTAGAAAGGACATATCTGAATGGCAGCCATCAAAAGGGTGGAATGATTG- GGAGAATG TGCCCTTCTGTTCCCACCACTTCCATGAACTACAGCTGAAGGATGGCAGGAGGATTGTGGTGCCTTGCCGAGAA- CAGGACGAG CTCATTGGGAGAGGAAGGGTGTCTCCAGGAAACGGCTGGATGATCAAGGAAACAGCTTGCCTCAGCAAAGCCTA- TGCCAACA TGTGGTCACTGATGTATTTTCACAAAAGGGACATGAGGCTACTGTCATTGGCTGTTTCCTCAGCTGTTCCCACC- TCATGGGTTCC ACAAGGACGCACAACATGGTCGATTCATGGGAAAGGGGAGTGGATGACCACGGAAGACATGCTTGAGGTGTGGA- ACAGAGT ATGGATAACCAACAACCCACACATGCAGGACAAGACAATGGTGAAAAAATGGAGAGATGTCCCTTATCTAACCA- AGAGACAA GACAAGCTGTGCGGATCACTGATTGGAATGACCAATAGGGCCACCTGGGCCTCCCACATCCATTTGGTCATCCA- TCGTATCCGA ACGCTGATTGGACAGGAGAAATACACTGACTACCTAACAGTCATGGACAGGTATTCTGTGGATGCTGACCTGCA- ACTGGGTGA GCTTATCTGAAACACCATCTAACAGGAATAACCGGGATACAAACCACGGGTGGAGAACCGGACTCCCCACAACC- TGAAACCG GGATATAAACCACGGCTGGAGAACCGGACTCCGCACTTAAAATGAAACAGAAACCGGGATAAAAACTACGGATG- GAGAACC GGACTCCACACATTGAGACAGAAGAAGTTGTCAGCCCAGAACCCCACACGAGTTTTGCCACTGCTAAGCTGTGA- GGCAGTGCA GGCTGGGACAGCCGACCTCCAGGTTGCGAAAAACCTGGTTTCTGGGACCTCCCACCCCAGAGTAAAAAGAACGG- AGCCTCCG CTACCACCCTCCCACGTGGTGGTAGAAAGACGGGGTCTAGAGGTTAGAGGAGACCCTCCAGGGAACAAATAGTG- GGACCATA TTGACGCCAGGGAAAGACCGGAGTGGTTCTCTGCTTTTCCTCCAGAGGTCTGTGAGCACAGTTTGCTCAAGAAT- AAGCAGACC TTTGGATGACAAA Attenuated Chikungunya "Delta5nsP3" sequence SEQ ID NO: 77 GATGGCTGCGTGAGACACACGTAGCCTACCAGTTTCTTACTGCTCTACTCTGCAAAGCAAGAGATTAATAACCC- ATCATGGATC CTGTGTACGTGGACATAGACGCTGACAGCGCCTTTTTGAAGGCCCTGCAACGTGCGTACCCCATGTTTGAGGTG- GAACCAAGG CAGGTCACACCGAATGACCATGCTAATGCTAGAGCGTTCTCGCATCTAGCTATAAAACTAATAGAGCAGGAAAT- TGACCCCGA CTCAACCATCCTGGATATCGGCAGTGCGCCAGCAAGGAGGATGATGTCGGACAGGAAGTACCACTGCGTCTGCC- CGATGCGC AGTGCGGAAGATCCCGAGAGACTCGCCAATTATGCGAGAAAGCTAGCATCTGCCGCAGGAAAAGTCCTGGACAG- AAACATCT CTGGAAAGATCGGGGACTTACAAGCAGTAATGGCCGTGCCAGACACGGAGACGCCAACATTCTGCTTACACACA- GACGTCTCA TGTAGACAGAGAGCAGACGTCGCTATATACCAAGACGTCTATGCTGTACACGCACCCACGTCGCTATACCACCA- GGCGATTAA AGGGGTCCGAGTGGCGTACTGGGTTGGGTTCGACACAACCCCGTTCATGTACAATGCCATGGCGGGTGCCTACC- CCTCATACT CGACAAACTGGGCAGATGAGCAGGTACTGAAGGCTAAGAACATAGGATTATGTTCAACAGACCTGACGGAAGGT- AGACGAG GCAAGTTGTCTATTATGAGAGGGAAAAAGCTAAAACCGTGCGACCGTGTGCTGTTCTCAGTAGGGTCAACGCTC- TACCCGGAA AGCCGCAAGCTACTTAAGAGCTGGCACCTGCCATCGGTGTTCCATTTAAAGGGCAAACTCAGCTTCACATGCCG- CTGTGATACA GTGGTTTCGTGTGAGGGCTACGTCGTTAAGAGAATAACGATGAGCCCAGGCCTTTATGGAAAAACCACAGGGTA- TGCGGTAA CCCACCACGCAGACGGATTCCTGATGTGCAAGACTACCGACACGGTTGACGGCGAAAGAATGTCATTCTCGGTG- TGCACATAC GTGCCGGCGACCATTTGTGATCAAATGACCGGCATCCTTGCTACAGAAGTCACGCCGGAGGATGCACAGAAGCT- GTTGGTGG GGCTGAACCAGAGAATAGTGGTTAACGGCAGAACGCAACGGAATACGAACACCATGAAAAATTATCTGCTTCCC- GTGGTCGC CCAAGCCTTCAGTAAGTGGGCAAAGGAGTGCCGGAAAGACATGGAAGATGAAAAACTCCTGGGGGTCAGAGAAA- GAACACT GACCTGCTGCTGTCTATGGGCATTCAAGAAGCAGAAAACACACACGGTCTACAAGAGGCCTGATACCCAGTCAA- TTCAGAAGG TTCAGGCCGAGTTTGACAGCTTTGTGGTACCGAGTCTGTGGTCGTCCGGGTTGTCAATCCCTTTGAGGACTAGA- ATCAAATGGT TGTTAAGCAAGGTGCCAAAAACCGACCTGATCCCATACAGCGGAGACGCCCGAGAAGCCCGGGACGCAGAAAAA- GAAGCAG AGGAAGAACGAGAAGCAGAACTGACTCGCGAAGCCCTACCACCTCTACAGGCAGCACAGGAAGATGTTCAGGTC- GAAATCGA CGTGGAACAGCTTGAGGACAGAGCGGGCGCAGGAATAATAGAGACTCCGAGAGGAGCTATCAAAGTTACTGCCC- AACCAAC AGACCACGTCGTGGGAGAGTACCTGGTACTCTCCCCGCAGACCGTACTACGTAGCCAGAAGCTCAGTCTGATTC- ACGCTTTGG CGGAGCAAGTGAAGACGTGCACGCACAACGGACGAGCAGGGAGGTATGCGGTCGAAGCGTACGACGGCCGAGTC- CTAGTGC CCTCAGGCTATGCAATCTCGCCTGAAGACTTCCAGAGTCTAAGCGAAAGCGCAACGATGGTGTATAACGAAAGA- GAGTTCGTA AACAGAAAGCTACACCATATTGCGATGCACGGACCAGCCCTGAACACCGACGAAGAGTCGTATGAGCTGGTGAG- GGCAGAGA GGACAGAACACGAGTACGTCTACGACGTGGATCAGAGAAGATGCTGTAAGAAGGAAGAAGCCGCAGGACTGGTA- CTGGTGG GCGACTTGACTAATCCGCCCTACCACGAATTCGCATATGAAGGGCTAAAAATCCGCCCTGCCTGCCCATACAAA- ATTGCAGTCA TAGGAGTCTTCGGAGTACCGGGATCTGGCAAGTCAGCTATTATCAAGAACCTAGTTACCAGGCAGGACCTGGTG- ACTAGCGG AAAGAAAGAAAACTGCCAAGAAATCACCACCGACGTGATGAGACAGAGAGGTCTAGAGATATCTGCACGTACGG- TTGACTCG CTGCTCTTGAATGGATGCAACAGACCAGTCGACGTGTTGTACGTAGACGAGGCGTTTGCGTGCCACTCTGGAAC- GCTACTTGC TTTGATCGCCTTGGTGAGACCAAGGCAGAAAGTTGTACTTTGTGGTGACCCGAAGCAGTGCGGCTTCTTCAATA- TGATGCAGA TGAAAGTCAACTATAATCACAACATCTGCACCCAAGTGTACCACAAAAGTATCTCCAGGCGGTGTACACTGCCT- GTGACCGCCA TTGTGTCATCGTTGCATTACGAAGGCAAAATGCGCACTACGAATGAGTACAACAAGCCGATTGTAGTGGACACT- ACAGGCTCA ACAAAACCTGACCCTGGAGACCTCGTGTTAACGTGCTTCAGAGGGTGGGTTAAACAACTGCAAATTGACTATCG- TGGATACGA GGTCATGACAGCAGCCGCATCCCAAGGGTTAACCAGAAAAGGAGTTTACGCAGTTAGACAAAAAGTTAATGAAA- ACCCGCTCT ATGCATCAACGTCAGAGCACGTCAACGTACTCCTAACGCGTACGGAAGGTAAACTGGTATGGAAGACACTTTCC- GGCGACCCG TGGATAAAGACGCTGCAGAACCCACCGAAAGGAAACTTCAAAGCAACTATTAAGGAGTGGGAGGTGGAGCATGC- ATCAATAA TGGCGGGCATCTGCAGTCACCAAATGACCTTCGATACATTCCAAAATAAAGCCAACGTTTGTTGGGCTAAGAGC- TTGGTCCCTA TCCTCGAAACAGCGGGGATAAAACTAAATGATAGGCAGTGGTCTCAGATAATTCAAGCCTTCAAAGAAGACAAA- GCATACTCA CCTGAAGTAGCCCTGAATGAAATATGTACGCGCATGTATGGGGTGGATCTAGACAGCGGGCTATTTTCTAAACC- GTTGGTGTC TGTGTATTACGCGGATAACCACTGGGATAATAGGCCTGGAGGGAAAATGTTCGGATTTAACCCCGAGGCAGCAT- CCATTCTAG AAAGAAAGTATCCATTCACAAAAGGGAAGTGGAACATCAACAAGCAGATCTGCGTGACTACCAGGAGGATAGAA- GACTTTAA CCCTACCACCAACATCATACCGGCCAACAGGAGACTACCACACTCATTAGTGGCCGAACACCGCCCAGTAAAAG- GGGAAAGAA TGGAATGGCTGGTTAACAAGATAAACGGCCACCACGTGCTCCTGGTCAGTGGCTATAACCTTGCACTGCCTACT- AAGAGAGTC ACTTGGGTAGCGCCGTTAGGTGTCCGCGGAGCGGACTACACATACAACCTAGAGTTGGGTCTGCCAGCAACGCT- TGGTAGGT ATGACCTAGTGGTCATAAACATCCACACACCTTTTCGCATACACCATTACCAACAGTGCGTCGACCACGCAATG- AAACTGCAAA TGCTCGGGGGTGACTCATTGAGACTGCTCAAACCGGGCGGCTCTCTATTGATCAGAGCATATGGTTACGCAGAT- AGAACCAGT GAACGAGTCATCTGCGTATTGGGACGCAAGTTTAGATCGTCTAGAGCGTTGAAACCACCATGTGTCACCAGCAA- CACTGAGAT GTTTTTCCTATTCAGCAACTTTGACAATGGCAGAAGGAATTTCACAACTCATGTCATGAACAATCAACTGAATG- CAGCCTTCGTA GGACAGGTCACCCGAGCAGGATGTGCACCGTCGTACCGGGTAAAACGCATGGACATCGCGAAGAACGATGAAGA- GTGCGTA GTCAACGCCGCTAACCCTCGCGGGTTACCGGGTGGCGGTGTTTGCAAGGCAGTATACAAAAAATGGCCGGAGTC- CTTTAAGA ACAGTGCAACACCAGTGGGAACCGCAAAAACAGTTATGTGCGGTACGTATCCAGTAATCCACGCTGTTGGACCA- AACTTCTCT AATTATTCGGAGTCTGAAGGGGACCGGGAATTGGCAGCTGCCTATCGAGAAGTCGCAAAGGAAGTAACTAGGCT- GGGAGTA AATAGTGTAGCTATACCTCTCCTCTCCACAGGTGTATACTCAGGAGGGAAAGACAGGCTGACCCAGTCACTGAA- CCACCTCTTT ACAGCCATGGACTCGACGGATGCAGACGTGGTCATCTACTGCCGCGACAAAGAATGGGAGAAGAAAATATCTGA- GGCCATAC AGATGCGGACCCAAGTAGAGCTGCTGGATGAGCACATCTCCATAGACTGCGATATTGTTCGCGTGCACCCTGAC- AGCAGCTTG GCAGGCAGAAAAGGATACAGCACCACGGAAGGCGCACTGTACTCATATCTAGAAGGGACCCGTTTTCATCAGAC- GGCTGTGG ATATGGCGGAGATACATACTATGTGGCCAAAGCAAACAGAGGCCAATGAGCAAGTCTGCCTATATGCCCTGGGG- GAAAGTAT TGAATCGATCAGGCAGAAATGCCCGGTGGATGATGCAGACGCATCATCTCCCCCCAAAACTGTCCCGTGCCTTT- GCCGTTACGC TATGACTCCAGAACGCGTCACCCGGCTTCGCATGAACCACGTCACAAGCATAATTGTGTGTTCTTCGTTTCCCC- TCCCAAAGTAC AAAATAGAAGGAGTGCAAAAAGTCAAATGCTCTAAGGTAATGCTATTTGACCACAACGTGCCATCGCGCGTAAG- TCCAAGGG CTTATAGAGGTGCCGCTGCCGGTAACCTTGCGGCCGTGTCTGATTGGGTAATGAGCACCGTACCTGTCGCGCCG- CCCAGAAGA AGGCGAGGGAGAAACCTGACTGTGACATGTGACGAGAGAGAAGGGAATATAACACCCATGGCTAGCGTCCGATT- CTTTAGG GCAGAGCTGTGTCCGGTCGTACAAGAAACAGCGGAGACGCGTGACACAGCAATGTCTCTTCAGGCACCACCGAG- TACCGCCA

CGGAACCGAATCATCCGCCGATCTCCTTCGGAGCATCAAGCGAGACGTTCCCCATTACATTTGGGGACTTCAAC- GAAGGAGAA ATCGAAAGCTTGTCTTCTGAGCTACTAACTTTCGGAGACTTCTTACCAGGAGAAGTGGATGACTTGACAGACAG- CGACTGGTC CACGTGCTCAGACACGGACGACGAGTTAAGACTAGACAGGGCAGGTGGGTATATATTCTCGTCGGACACCGGTC- CAGGTCAT TTACAACAGAAGTCAGTACGCCAGTCAGTGCTGCCGGTGAACACCCTGGAGGAAGTCCACGAGGAGAAGTGTTA- CCCACCTA AGCTGGATGAAGCAAAGGAGCAACTATTACTTAAGAAACTCCAGGAGAGTGCATCCATGGCCAACAGAAGCAGG- TATCAGTC GCGCAAAGTAGAAAACATGAAAGCAGCAATCATCCAGAGACTAAAGAGAGGCTGTAGACTATACTTAATGTCAG- AGACCCCA AAAGTCCCTACTTACCGGACTACATATCCGGCGCCTGTGTACTCGCCTCCGATCAACGTCCGATTGTCCAATCC- CGAGTCCGCA GTGGCAGCATGCAATGAGTTCTTAGCTAGAAACTATCCAACTGTCTCATCATACCAAATTACCGACGAGTATGA- TGCATATCTA GACATGGTGGACGGGTCGGAGAGTTGCCTGGACCGAGCGACATTCAATCCGTCAAAACTCAGGAGCTACCCGAA- ACAGCACG CTTACCACGCGCCCTCCATCAGAAGCGCTGTACCGTCCCCATTCCAGAACACACTACAGAATGTACTGGCAGCA- GCCACGAAAA GAAACTGCAACGTCACACAGATGAGGGAATTACCCACTTTGGACTCAGCAGTATTCAACGTGGAGTGTTTCAAA- AAATTCGCA TGCAACCAAGAATACTGGGAAGAATTTGCTGCCAGCCCTATTAGGATAACAACTGAGAATTTAGCAACCTATGT- TACTAAACTA AAAGGGCCAAAAGCAGCAGCGCTATTCGCAAAAACCCATAATCTACTGCCACTACAGGAAGTACCAATGGATAG- GTTCACAGT AGATATGAAAAGGGACGTAAAGGTGACTCCTGGTACAAAGCATACAGAGGAAAGACCTAAGGTGCAGGTTATAC- AGGCGGC TGAACCCTTGGCGACAGCATACCTATGTGGGATTCACAGAGAGCTGGTTAGGAGGCTGAACGCCGTCCTCCTAC- CCAATGTAC ATACACTATTTGACATGTCTGCCGAGGATTTCGATGCCATCATAGCCGCACACTTTAAGCCAGGAGACACTGTT- TTGGAAACGG ACATAGCCTCCTTTGATAAGAGCCAAGATGATTCACTTGCGCTTACTGCTTTGATGCTGTTAGAGGATTTAGGG- GTGGATCACT CCCTGCTGGACTTGATAGAGGCTGCTTTCGGAGAGATTTCCAGCTGTCACCTACCGACAGGTACGCGCTTCAAG- TTCGGCGCC ATGATGAAATCAGGTATGTTCCTAACTCTGTTCGTCAACACATTGTTAAACATCACCATCGCCAGCCGAGTGCT- GGAAGATCGT CTGACAAAATCCGCGTGCGCGGCCTTCATCGGCGACGACAACATAATACATGGAGTCGTCTCCGATGAATTGAT- GGCAGCCAG ATGTGCCACTTGGATGAACATGGAAGTGAAGATCATAGATGCAGTTGTATCCTTGAAAGCCCCTTACTTTTGTG- GAGGGTTTAT ACTGCACGATACTGTGACAGGAACAGCTTGCAGAGTGGCAGACCCGCTAAAAAGGCTTTTTAAACTGGGCAAAC- CGCTAGCG GCAGGTGACGAACAAGATGAAGATAGAAGACGAGCGCTGGCTGACGAAGTGATCAGATGGCAACGAACAGGGCT- AATTGAT GAGCTGGAGAAAGCGGTATACTCTAGGTACGAAGTGCAGGGTATATCAGTTGTGGTAATGTCCATGGCCACCTT- TGCAAGCTC CAGATCCAACTTCGAGAAGCTCAGAGGACCCGTCATAACTTTGTACGGCGGTCCTAAATAGGTACGCACTACAG- CTACCTATTT TGCAGAAGCCGACAGCAAGTATCTAAACACTAATCAGCTACAATGGAGTTCATCCCAACCCAAACTTTTTACAA- TAGGAGGTAC CAGCCTCGACCCTGGACTCCGCGCCCTACTATCCAAGTCATCAGGCCCAGACCGCGCCCTCAGAGGCAAGCTGG- GCAACTTGC CCAGCTGATCTCAGCAGTTAATAAACTGACAATGCGCGCGGTACCACAACAGAAGCCACGCAGGAATCGGAAGA- ATAAGAAG CAAAAGCAAAAACAACAGGCGCCACAAAACAACACAAATCAAAAGAAGCAGCCACCTAAAAAGAAACCGGCTCA- AAAGAAAA AGAAGCCGGGCCGCAGAGAGAGGATGTGCATGAAAATCGAAAATGATTGTATTTTCGAAGTCAAGCACGAAGGT- AAGGTAA CAGGTTACGCGTGCCTGGTGGGGGACAAAGTAATGAAACCAGCACACGTAAAGGGGACCATCGATAACGCGGAC- CTGGCCA AACTGGCCTTTAAGCGGTCATCTAAGTATGACCTTGAATGCGCGCAGATACCCGTGCACATGAAGTCCGACGCT- TCGAAGTTC ACCCATGAGAAACCGGAGGGGTACTACAACTGGCACCACGGAGCAGTACAGTACTCAGGAGGCCGGTTCACCAT- CCCTACAG GTGCTGGCAAACCAGGGGACAGCGGCAGACCGATCTTCGACAACAAGGGACGCGTGGTGGCCATAGTCTTAGGA- GGAGCTA ATGAAGGAGCCCGTACAGCCCTCTCGGTGGTGACCTGGAATAAAGACATTGTCACTAAAATCACCCCCGAGGGG- GCCGAAGA GTGGAGTCTTGCCATCCCAGTTATGTGCCTGTTGGCAAACACCACGTTCCCCTGCTCCCAGCCCCCTTGCACGC- CCTGCTGCTAC GAAAAGGAACCGGAGGAAACCCTACGCATGCTTGAGGACAACGTCATGAGACCTGGGTACTATCAGCTGCTACA- AGCATCCTT AACATGTTCTCCCCACCGCCAGCGACGCAGCACCAAGGACAACTTCAATGTCTATAAAGCCACAAGACCATACT- TAGCTCACTG TCCCGACTGTGGAGAAGGGCACTCGTGCCATAGTCCCGTAGCACTAGAACGCATCAGAAATGAAGCGACAGACG- GGACGCTG AAAATCCAGGTCTCCTTGCAAATCGGAATAAAGACGGATGACAGCCACGATTGGACCAAGCTGCGTTATATGGA- CAACCACAT GCCAGCAGACGCAGAGAGGGCGGGGCTATTTGTAAGAACATCAGCACCGTGTACGATTACTGGAACAATGGGAC- ACTTCATC CTGGCCCGATGTCCAAAAGGGGAAACTCTGACGGTGGGATTCACTGACAGTAGGAAGATTAGTCACTCATGTAC- GCACCCATT TCACCACGACCCTCCTGTGATAGGTCGGGAAAAATTCCATTCCCGACCGCAGCACGGTAAAGAGCTACCTTGCA- GCACGTACG TGCAGAGCACCGCCGCAACTACCGAGGAGATAGAGGTACACATGCCCCCAGACACCCCTGATCGCACATTAATG- TCACAACAG TCCGGCAACGTAAAGATCACAGTCAATGGCCAGACGGTGCGGTACAAGTGTAATTGCGGTGGCTCAAATGAAGG- ACTAACAA CTACAGACAAAGTGATTAATAACTGCAAGGTTGATCAATGTCATGCCGCGGTCACCAATCACAAAAAGTGGCAG- TATAACTCC CCTCTGGTCCCGCGTAATGCTGAACTTGGGGACCGAAAAGGAAAAATTCACATCCCGTTTCCGCTGGCAAATGT- AACATGCAG GGTGCCTAAAGCAAGGAACCCCACCGTGACGTACGGGAAAAACCAAGTCATCATGCTACTGTATCCTGACCACC- CAACACTCC TGTCCTACCGGAATATGGGAGAAGAACCAAACTATCAAGAAGAGTGGGTGATGCATAAGAAGGAAGTCGTGCTA- ACCGTGCC GACTGAAGGGCTCGAGGTCACGTGGGGCAACAACGAGCCGTATAAGTATTGGCCGCAGTTATCTACAAACGGTA- CAGCCCAT GGCCACCCGCATGAGATAATTCTGTATTATTATGAGCTGTACCCCACTATGACTGTAGTAGTTGTGTCAGTGGC- CACGTTCATA CTCCTGTCGATGGTGGGTATGGCAGCGGGGATGTGCATGTGTGCACGACGCAGATGCATCACACCGTATGAACT- GACACCAG GAGCTACCGTCCCTTTCCTGCTTAGCCTAATATGCTGCATCAGAACAGCTAAAGCGGCCACATACCAAGAGGCT- GCGATATACC TGTGGAACGAGCAGCAACCTTTGTTTTGGCTACAAGCCCTTATTCCGCTGGCAGCCCTGATTGTTCTATGCAAC- TGTCTGAGAC TCTTACCATGCTGCTGTAAAACGTTGGCTTTTTTAGCCGTAATGAGCGTCGGTGCCCACACTGTGAGCGCGTAC- GAACACGTAA CAGTGATCCCGAACACGGTGGGAGTACCGTATAAGACTCTAGTCAATAGACCTGGCTACAGCCCCATGGTATTG- GAGATGGA ACTACTGTCAGTCACTTTGGAGCCAACACTATCGCTTGATTACATCACGTGCGAGTACAAAACCGTCATCCCGT- CTCCGTACGT GAAGTGCTGCGGTACAGCAGAGTGCAAGGACAAAAACCTACCTGACTACAGCTGTAAGGTCTTCACCGGCGTCT- ACCCATTTA TGTGGGGCGGCGCCTACTGCTTCTGCGACGCTGAAAACACGCAGTTGAGCGAAGCACACGTGGAGAAGTCCGAA- TCATGCAA AACAGAATTTGCATCAGCATACAGGGCTCATACCGCATCTGCATCAGCTAAGCTCCGCGTCCTTTACCAAGGAA- ATAACATCAC TGTAACTGCCTATGCAAACGGCGACCATGCCGTCACAGTTAAGGACGCCAAATTCATTGTGGGGCCAATGTCTT- CAGCCTGGA CACCTTTCGACAACAAAATTGTGGTGTACAAAGGTGACGTCTATAACATGGACTACCCGCCCTTTGGCGCAGGA- AGACCAGGA CAATTTGGCGATATCCAAAGTCGCACACCTGAGAGTAAAGACGTCTATGCTAATACACAACTGGTACTGCAGAG- ACCGGCTGT GGGTACGGTACACGTGCCATACTCTCAGGCACCATCTGGCTTTAAGTATTGGCTAAAAGAACGCGGGGCGTCGC- TGCAGCACA CAGCACCATTTGGCTGCCAAATAGCAACAAACCCGGTAAGAGCGGTGAACTGCGCCGTAGGGAACATGCCCATC- TCCATCGAC ATACCGGAAGCGGCCTTCACTAGGGTCGTCGACGCGCCCTCTTTAACGGACATGTCGTGCGAGGTACCAGCCTG- CACCCATTC CTCAGACTTTGGGGGCGTCGCCATTATTAAATATGCAGCCAGCAAGAAAGGCAAGTGTGCGGTGCATTCGATGA- CTAACGCCG TCACTATTCGGGAAGCTGAGATAGAAGTTGAAGGGAATTCTCAGCTGCAAATCTCTTTCTCGACGGCCTTAGCC- AGCGCCGAA TTCCGCGTACAAGTCTGTTCTACACAAGTACACTGTGCAGCCGAGTGCCACCCCCCGAAGGACCACATAGTCAA- CTACCCGGC GTCACATACCACCCTCGGGGTCCAGGACATCTCCGCTACGGCGATGTCATGGGTGCAGAAGATCACGGGAGGTG- TGGGACTG GTTGTTGCTGTTGCCGCACTGATTCTAATCGTGGTGCTATGCGTGTCGTTCAGCAGGCACTAACTTGACAATTA- AGTATGAAGG TATATGTGTCCCCTAAGAGACACACTGTACATAGCAAATAATCTATAGATCAAAGGGCTACGCAACCCCTGAAT- AGTAACAAAA TACAAAATCACTAAAAATTATAAAAACAGAAAAATACATAAATAGGTATACGTGTCCCCTAAGAGACACATTGT- ATGTAGGTG ATAAGTATAGATCAAAGGGCCGAATAACCCCTGAATAGTAACAAAATATGAAAATCAATAAAAATCATAAAATA- GAAAAACCA TAAACAGAAGTAGTTCAAAGGGCTATAAAACCCCTGAATAGTAACAAAACATAAAATTAATAAAAATCAAATGA- ATACCATAA TTGGCAAACGGAAGAGATGTAGGTACTTAAGCTTCCTAAAAGCAGCCGAACTCACTTTGAGAAGTAGGCATAGC- ATACCGAAC TCTTCCACGATTCTCCGAACCCACAGGGACGTAGGAGATGTTATTTTGTTTTTAATATTTCAAAAAAAAAAAAA- AAAAAAAAAAA ZIKV Sequence H/PF/2013 as sequenced SEQ ID NO: 78 CAGACTGCGACAGTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTTTATTTTGGATTTGGAAACG- AGAGTTTCT GGTCATGAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATATGCTAAAACGCGGAGTAGCCCGTG- TGAGCCCC TTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGTCATGGGCCCATCAGGATGGTCTTGGCGATTCT- AGCCTTTTT GAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGG- AAATAATA AAGAAGTTCAAGAAAGATCTGGCTGCCATGCTGAGAATAATCAATGCTAGGAAGGAGAAGAAGAGACGAGGCGC- AGATACT AGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGCGGAGGTCACTAGACGTGGGAGTGCATACTA- TATGTACTT

GGACAGAAACGACGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAAGTGTTATATACAGATCA- TGGATCTTG GACACATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGAT- TGTTGGTG CAACACGACGTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAAAAGGTGAAGCACGGAGATCTAGAAGAG- CTGTGACG CTCCCCTCCCATTCCACTAGGAAGCTGCAAACGCGGTCGCAAACCTGGTTGGAATCAAGAGAATACACAAAGCA- CTTGATTAG AGTCGAAAATTGGATATTCAGGAACCCTGGCTTCGCGTTAGCAGCAGCTGCCATCGCTTGGCTTTTGGGAAGCT- CAACGAGCC AAAAAGTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTCAGCAAT- AGGGACTTT GTGGAAGGTATGTCAGGTGGGACTTGGGTTGATGTTGTCTTGGAACATGGAGGTTGTGTCACCGTAATGGCACA- GGACAAAC CGACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGTAAGATCCTACTGCTATGAGGCA- TCAATATCG GACATGGCTTCGGACAGCCGCTGCCCAACACAAGGTGAAGCCTACCTTGACAAGCAATCAGACACTCAATATGT- CTGCAAAAG AACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGCCTGGTGACATGCGCTAAGT- TTGCATGC TCCAAGAAAATGACCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAATGCTGTCAGTTCATGGCTC- CCAGCACA GTGGGATGATCGTTAATGACACAGGACATGAAACTGATGAGAATAGAGCGAAGGTTGAGATAACGCCCAATTCA- CCAAGAGC CGAAGCCACCCTGGGGGGTTTTGGAAGCCTAGGACTTGATTGTGAACCGAGGACAGGCCTTGACTTTTCAGATT- TGTATTACT TGACTATGAATAACAAGCACTGGTTGGTTCACAAGGAGTGGTTCCACGACATTCCATTACCTTGGCACGCTGGG- GCAGACACC GGAACTCCACACTGGAACAACAAAGAAGCACTGGTAGAGTTCAAGGACGCACATGCCAAAAGGCAAACTGTCGT- GGTTCTAG GGAGTCAAGAAGGAGCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGGATGGTGCAAAGGGAAGG- CTGTCCT CTGGCCACTTGAAATGTCGCCTGAAAATGGATAAACTTAGATTGAAGGGCGTGTCATACTCCTTGTGTACCGCA- GCGTTCACAT TCACCAAGATCCCGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGTACAGTACGCAGGGACAGATGGACCT- TGCAAGGT TCCAGCTCAGATGGCGGTGGACATGCAAACTCTGACCCCAGTTGGGAGGTTGATAACCGCTAACCCCGTAATCA- CTGAAAGCA CTGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACATTGTCATAGGAGTCGGGGAG- AAGAAGATC ACCCACCACTGGCACAGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGAGAGGTGCCAAGAGAAT- GGCAGTCT TGGGAGACACAGCCTGGGACTTTGGATCAGTTGGAGGCGCTCTCAACTCATTGGGCAAGGGCATCCATCAAATT- TTTGGAGCA GCTTTCAAATCATTGTTTGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGAACGTTGCTGATGTGGTTGGG- TCTGAACACA AAGAATGGATCTATTTCCCTTATGTGCTTGGCCTTAGGGGGAGTGTTGATCTTCTTATCCACAGCTGTCTCTGC- TGATGTGGGG TGCTCGGTGGACTTCTCAAAGAAGGAGACGAGATGCGGTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTG- GAGGGACA GGTACAAGTACCATCCTGACTCCCCCCGTAGATTGGCAGCAGCAGTCAAGCAAGCCTGGGAAGATGGTATCTGT- GGGATCTCC TCTGTTTCAAGAATGGAAAACATCATGTGGAGATCAGTAGAAGGGGAGCTCAACGCAATCCTGGAAGAGAATGG- AGTTCAAC TGACGGTCGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATTGCCCGTGCCTGTGAACGAG- CTGCCCCAC GGCTGGAAGGCTTGGGGGAAATCGTACTTCGTCAGAGCAGCAAAGACAAATAACAGCTTTGTCGTGGATGGTGA- CACACTGA AGGAATGCCCACTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAGGATCATGGGTTCGGGGTATTTCACACT- AGTGTCTGG CTCAAGGTTAGAGAAGATTATTCATTAGAGTGTGATCCAGCCGTTATTGGAACAGCTGTTAAGGGAAAGGAGGC- TGTACACA GTGATCTAGGCTACTGGATTGAGAGTGAGAAGAATGACACATGGAGGCTGAAGAGGGCCCATCTGATCGAGATG- AAAACAT GTGAATGGCCAAAGTCCCACACATTGTGGACAGATGGAATAGAAGAGAGTGATCTGATCATACCCAAGTCTTTA- GCTGGGCCA CTCAGCCATCACAATACCAGAGAGGGCTACAGGACCCAAATGAAAGGGCCATGGCACAGTGAAGAGCTTGAAAT- TCGGTTTG AGGAATGCCCAGGCACTAAGGTCCACGTGGAGGAAACATGTGGAACAAGAGGACCATCTCTGAGATCAACCACT- GCAAGCGG AAGGGTGATCGAGGAATGGTGCTGCAGGGAGTGCACAATGCCCCCACTGTCGTTCCGGGCTAAAGATGGCTGTT- GGTATGGA ATGGAGATAAGGCCCAGGAAAGAACCAGAAAGTAACTTAGTAAGGTCAATGGTGACTGCAGGATCAACTGATCA- CATGGATC ACTTCTCCCTTGGAGTGCTTGTGATTCTGCTCATGGTGCAGGAAGGGCTGAAGAAGAGAATGACCACAAAGATC- ATCATAAGC ACATCGATGGCAGTGCTGGTAGCTATGATCCTGGGAGGATTTTCAATGAGTGACCTGGCTAAGCTTGCAATTTT- GATGGGTGC CACCTTCGCGGAAATGAACACTGGAGGAGATGTAGCTCATCTGGCGCTGATAGCGGCATTCAAAGTCAGACCAG- CGTTGCTG GTATCTTTCATCTTCAGAGCTAATTGGACACCCCGTGAAAGCATGCTGCTGGCCTTGGCCTCGTGTCTTTTGCA- AACTGCGATCT CCGCCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGGTTGGCAATACGAGCGATGGTT- GTTCCACGCA CTGATAACATCACCTTGGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCACACTGCTTGTGGCGTGGAGA- GCAGGCCTT GCTACTTGCGGGGGGTTTATGCTCCTCTCTCTGAAGGGAAAAGGCAGTGTGAAGAAGAACTTACCATTTGTCAT- GGCCCTGGG ACTAACCGCTGTGAGGCTGGTCGACCCCATCAACGTGGTGGGACTGCTGTTGCTCACAAGGAGTGGGAAGCGGA- GCTGGCCC CCTAGCGAAGTACTCACAGCTGTTGGCCTGATATGCGCATTGGCTGGAGGGTTCGCCAAGGCAGATATAGAGAT- GGCTGGGC CCATGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAGGAAAGAGTGTGGACATGTACATTGAAAGA- GCAGGTGA CATCACATGGGAAAAAGATGCGGAAGTCACTGGAAACAGTCCCCGGCTCGATGTGGCGCTAGATGAGAGTGGTG- ATTTCTCC CTGGTGGAGGATGACGGTCCCCCCATGAGAGAGATCATACTCAAGGTGGTCCTGATGACCATCTGTGGCATGAA- CCCAATAGC CATACCCTTTGCAGCTGGAGCGTGGTACGTATACGTGAAGACTGGAAAAAGGAGTGGTGCTCTATGGGATGTGC- CTGCTCCCA AGGAAGTAAAAAAGGGGGAGACCACAGATGGAGTGTACAGAGTAATGACTCGTAGACTGCTAGGTTCAACACAA- GTTGGAG TGGGAGTTATGCAAGAGGGGGTCTTTCACACTATGTGGCACGTCACAAAAGGATCCGCGCTGAGAAGCGGTGAA- GGGAGAC TTGATCCATACTGGGGAGATGTCAAGCAGGATCTGGTGTCATACTGTGGTCCATGGAAGCTAGATGCCGCCTGG- GACGGGCA CAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGAGAGCGAGGAACATCCAGACTCTGCCCGGAATATTTA- AGACAAAG GATGGGGACATTGGAGCGGTTGCGCTGGATTACCCAGCAGGAACTTCAGGATCTCCAATCCTAGACAAGTGTGG- GAGAGTGA TAGGACTTTATGGCAATGGGGTCGTGATCAAAAATGGGAGTTATGTTAGTGCCATCACCCAAGGGAGGAGGGAG- GAAGAGA CTCCTGTTGAGTGCTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTAACTGTCTTAGACTTGCATCCTGGAGCT- GGGAAAACC AGGAGAGTTCTTCCTGAAATAGTCCGTGAAGCCATAAAAACAAGACTCCGTACTGTGATCTTAGCTCCAACCAG- GGTTGTCGCT GCTGAAATGGAGGAAGCCCTTAGAGGGCTTCCAGTGCGTTATATGACAACAGCAGTCAATGTCACCCACTCTGG- AACAGAAAT CGTCGACTTAATGTGCCATGCCACCTTCACTTCACGTCTACTACAGCCAATCAGAGTCCCCAACTATAATCTGT- ATATTATGGAT GAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAAGAGGATACATTTCAACAAGGGTTGAGATGGGCGAGGC- GGCTGCCA TCTTCATGACCGCCACGCCACCAGGAACCCGTGACGCATTTCCGGACTCCAACTCACCAATTATGGACACCGAA- GTGGAAGTCC CAGAGAGAGCCTGGAGCTCAGGCTTTGATTGGGTGACGGATCATTCTGGAAAAACAGTTTGGTTTGTTCCAAGC- GTGAGGAA CGGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAACGGGTCATACAGCTCAGCAGAAAGACTTTTGAGA- CAGAGTTC CAGAAAACAAAACATCAAGAGTGGGACTTTGTCGTGACAACTGACATTTCAGAGATGGGCGCCAACTTTAAAGC- TGACCGTGT CATAGATTCCAGGAGATGCCTAAAGCCGGTCATACTTGATGGCGAGAGAGTCATTCTGGCTGGACCCATGCCTG- TCACACATG CCAGCGCTGCCCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGGAGATGAGTATCTGTATGGAGGT- GGGTGCG CAGAGACTGACGAAGACCATGCACACTGGCTTGAAGCAAGAATGCTCCTTGACAATATTTACCTCCAAGATGGC- CTCATAGCC TCGCTCTATCGACCTGAGGCCGACAAAGTAGCAGCCATTGAGGGAGAGTTCAAGCTTAGGACGGAGCAAAGGAA- GACCTTTG TGGAACTCATGAAAAGAGGAGATCTTCCTGTTTGGCTGGCCTATCAGGTTGCATCTGCCGGAATAACCTACACA- GATAGAAGA TGGTGCTTTGATGGCACGACCAACAACACCATAATGGAAGACAGTGTGCCGGCAGAGGTGTGGACCAGACACGG- AGAGAAA AGAGTGCTCAAACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCCTGAAGTCATTCAAGGAGTT- TGCCGCTG GGAAAAGAGGAGCGGCTTTTGGAGTGATGGAAGCCCTGGGAACACTGCCAGGACACATGACAGAGAGATTCCAG- GAAGCCA TTGACAACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGCCTTACAAAGCCGCGGCGGCCCAATTGCCG- GAGACCCT AGAGACCATTATGCTTTTGGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTTTTCGTCTTGATGAGGAACAAGG- GCATAGGGA AGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGCGCATGGCTCATGTGGCTCTCGGAAATTGAGCCAGCCAGA- ATTGCATGT GTCCTCATTGTTGTGTTCCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAAAGATCTCCCCAGGACAA- CCAAATGGCA ATCATCATCATGGTAGCAGTAGGTCTTCTGGGCTTGATTACCGCCAATGAACTCGGATGGTTGGAGAGAACAAA- GAGTGACCT AAGCCATCTAATGGGAAGGAGAGAGGAGGGGGCAACCATAGGATTCTCAATGGACATTGACCTGCGGCCAGCCT- CAGCTTGG GCCATCTATGCTGCCTTGACAACTTTCATTACCCCAGCCGTCCAACATGCAGTGACCACTTCATACAACAACTA- CTCCTTAATGG CGATGGCCACGCAAGCTGGAGTGTTGTTTGGTATGGGCAAAGGGATGCCATTCTACGCATGGGACTTTGGAGTC- CCGCTGCTA ATGATAGGTTGCTACTCACAATTAACACCCCTGACCCTAATAGTGGCCATCATTTTGCTCGTGGCGCACTACAT- GTACTTGATCC CAGGGCTGCAGGCAGCAGCTGCGCGTGCTGCCCAGAAGAGAACGGCAGCTGGCATCATGAAGAACCCTGTTGTG- GATGGAA TAGTGGTGACTGACATTGACACAATGACAATTGACCCCCAAGTGGAGAAAAAGATGGGACAGGTGCTACTCATA-

GCAGTAGC CGTCTCCAGCGCCATACTGTCGCGGACCGCCTGGGGGTGGGGGGAGGCTGGGGCCCTGATCACAGCGGCAACTT- CCACTTTG TGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCTACAGCCACTTCACTGTGTAACATTTTTAGGGGAAGTTA- CTTGGCTGGA GCTTCTCTAATCTACACAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGGTGGAACAGGAGAGACCCT- GGGAGAG AAATGGAAGGCCCGCTTGAACCAGATGTCGGCCCTGGAGTTCTACTCCTACAAAAAGTCAGGCATCACCGAGGT- GTGCAGAG AAGAGGCCCGCCGCGCCCTCAAGGACGGTGTGGCAACGGGAGGCCATGCTGTGTCCCGAGGAAGTGCAAAGCTG- AGATGGT TGGTGGAGCGGGGATACCTGCAGCCCTATGGAAAGGTCATTGATCTTGGATGTGGCAGAGGGGGCTGGAGTTAC- TACGCCGC CACCATCCGCAAAGTTCAAGAAGTGAAAGGATACACAAAAGGAGGCCCTGGTCATGAAGAACCCATGTTGGTGC- AAAGCTAT GGGTGGAACATAGTCCGTCTTAAGAGTGGGGTGGACGTCTTTCATATGGCGGCTGAGCCGTGTGACACGTTGCT- GTGTGACA TAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAAGCACGGACGCTCAGAGTCCTCTCCATGGTGGGGGATTGG- CTTGAAAA AAGACCAGGAGCCTTTTGTATAAAAGTGTTGTGCCCATACACCAGCACTATGATGGAAACCCTGGAGCGACTGC- AGCGTAGGT ATGGGGGAGGACTGGTCAGAGTGCCACTCTCCCGCAACTCTACACATGAGATGTACTGGGTCTCTGGAGCGAAA- AGCAACAC CATAAAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCGCATGGACGGGCCCAGGAGGCCAGTGAAATATG- AGGAGGAT GTGAATCTCGGCTCTGGCACGCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACATGAAGATCATTGGTAACCG- CATTGAAAG GATCCGCAGTGAGCACGCGGAAACGTGGTTCTTTGACGAGAACCACCCATATAGGACATGGGCTTACCATGGAA- GCTATGAG GCCCCCACACAAGGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGTCAAAACCCTGGGATGTGGT- GACTGGAGT CACAGGAATAGCCATGACCGACACCACACCGTATGGTCAGCAAAGAGTTTTCAAGGAAAAAGTGGACACTAGGG- TGCCAGAC CCCCAAGAAGGCACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTTGTGGAAAGAGCTAGGCAAACACAAACG- GCCACGAG TCTGTACCAAAGAAGAGTTCATCAACAAGGTTCGTAGCAATGCAGCATTAGGGGCAATATTTGAAGAGGAAAAA- GAGTGGAA GACTGCAGTGGAAGCTGTGAACGATCCAAGGTTCTGGGCTCTAGTGGACAAGGAAAGAGAGCACCACCTGAGAG- GAGAGTG CCAGAGTTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAACAAGGGGAATTTGGAAAGGCCAAGGGCAGCC- GCGCCAT CTGGTATATGTGGCTAGGGGCTAGATTTCTAGAGTTCGAAGCCCTTGGATTCTTGAACGAGGATCACTGGATGG- GGAGAGAG AACTCAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGATATGTCCTAGAAGAGATGAGTCGCATACC- AGGAGGA AGGATGTATGCAGATGACACTGCTGGCTGGGACACCCGCATCAGCAGGTTTGATCTGGAGAATGAAGCTCTAAT- CACCAACCA AATGGAGAAAGGGCACAGGGCCTTGGCATTGGCCATAATCAAGTACACATACCAAAACAAAGTGGTAAAGGTCC- TTAGACCA GCTGAAAAAGGGAAGACAGTTATGGACATTATTTCGAGACAAGACCAAAGGGGGAGCGGACAAGTTGTCACTTA- CGCTCTTA ACACATTTACCAACCTAGTGGTGCAACTCATTCGGAATATGGAGGCTGAGGAAGTTCTAGAGATGCAAGACTTG- TGGCTGCTG CGGAGGTCAGAGAAAGTGACCAACTGGTTGCAGAGCAACGGATGGGATAGGCTCAAACGAATGGCAGTCAGTGG- AGATGAT TGCGTTGTGAAGCCAATTGATGATAGGTTTGCACATGCCCTCAGGTTCTTGAATGATATGGGAAAAGTTAGGAA- GGACACACA AGAGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAAGTTCCGTTTTGCTCCCACCACTTCAACAAGCTCC- ATCTCAAGG ACGGGAGGTCCATTGTGGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCCCGCGTCTCTCCAGGGGCG- GGATGGAG CATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGCAAATGTGGCAGCTCCTTTATTTCCACAGAAGGGACC- TCCGACTGAT GGCCAATGCCATTTGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGGAGAACTACCTGGTCAATCCATGGAA- AGGGAGAAT GGATGACCACTGAAGACATGCTTGTGGTGTGGAACAGAGTGTGGATTGAGGAGAACGACCACATGGAAGACAAG- ACCCCAG TTACGAAATGGACAGACATTCCCTATTTGGGAAAAAGGGAAGACTTGTGGTGTGGATCTCTCATAGGGCACAGA- CCGCGCACC ACCTGGGCTGAGAACATTAAAAACACAGTCAACATGGTGCGCAGGATCATAGGTGATGAAGAAAAGTACATGGA- CTACCTAT CCACCCAAGTTCGCTACTTGGGTGAAGAAGGGTCTACACCTGGAGTGCTGTAAGCACCAATCTTAGTGTTGTCA- GGCCTGCTA GTCAGCCACAGCTTGGGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGAAGCTGGGAAACCAAGCCTATAGTC- AGGCCGAG AACGCCATGGCACGGAAGAAGCCATGCTGCCTGTGAGCCCCTCAGAGGACACTGAGTCAAAAAACCCCACGCGC- TTGGAGGC GCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAACTGGAGATCAGCTGTGGAT- CTCCAGAA GAGGGACTAGTGGTTAGAGGAGACCCCCCGGAAAACGCAAAACAGCATATTGACGCTGGGAAAGACCAGAGACT- CCATGAG TTTCCACCACGCTGGCCGCCAGGCACAGATCGCCGAATAGCGGCGGCCGGTGTGGGG AHZ13508.1, Zika virus polyprotein from Polynesian outbreak (H/PF/2013) SEQ ID NO: 79 MKNPKKKSGGFRIVNMLKRGVARVSPFGGLKRLPAGLLLGHGPIRMVLAILAFLRFTAIKPSLGLINRWGSVGK- KEAMEIIKKFKKDL AAMLRIINARKEKKRRGADTSVGIVGLLLTTAMAAEVTRRGSAYYMYLDRNDAGEAISFPTTLGMNKCYIQIMD- LGHMCDATMSYE CPMLDEGVEPDDVDCWCNTTSTWVVYGTCHHKKGEARRSRRAVTLPSHSTRKLQTRSQTWLESREYTKHLIRVE- NWIFRNPGFAL AAAAIAWLLGSSTSQKVIYLVMILLIAPAYSIRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDI- ELVTTTVSNMAE VRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKS- IQPENLEYRIML SVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHK- EWFHDIPLPWH AGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRL- KGVSYSLCTA AFTFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGD- SYIVIGVGEKKIT HHWHRSGSTIGKAFEATVRGAKRMAVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTL- LMWLGLNTKN GSISLMCLALGGVLIFLSTAVSADVGCSVDFSKKETRCGTGVFVYNDVEAWRDRYKYHPDSPRRLAAAVKQAWE- DGICGISSVSRME NIMWRSVEGELNAILEENGVQLTVVVGSVKNPMWRGPQRLPVPVNELPHGWKAWGKSYFVRAAKTNNSFVVDGD- TLKECPLKH RAWNSFLVEDHGFGVFHTSVWLKVREDYSLECDPAVIGTAVKGKEAVHSDLGYWIESEKNDTWRLKRAHLIEMK- TCEWPKSHTLW TDGIEESDLIIPKSLAGPLSHHNTREGYRTQMKGPWHSEELEIRFEECPGTKVHVEETCGTRGPSLRSTTASGR- VIEEWCCRECTMPPL SFRAKDGCWYGMEIRPRKEPESNLVRSMVTAGSTDHMDHFSLGVLVILLMVQEGLKKRMTTKIIISTSMAVLVA- MILGGFSMSDLA KLAILMGATFAEMNTGGDVAHLALIAAFKVRPALLVSFIFRANWTPRESMLLALASCLLQTAISALEGDLMVLI- NGFALAWLAIRAM VVPRTDNITLAILAALTPLARGTLLVAWRAGLATCGGFMLLSLKGKGSVKKNLPFVMALGLTAVRLVDPINVVG- LLLLTRSGKRSWPP SEVLTAVGLICALAGGFAKADIEMAGPMAAVGLLIVSYVVSGKSVDMYIERAGDITWEKDAEVTGNSPRLDVAL- DESGDFSLVEDD GPPMREIILKVVLMTICGMNPIAIPFAAGAWYVYVKTGKRSGALWDVPAPKEVKKGETTDGVYRVMTRRLLGST- QVGVGVMQEG VFHTMWHVTKGSALRSGEGRLDPYWGDVKQDLVSYCGPWKLDAAWDGHSEVQLLAVPPGERARNIQTLPGIFKT- KDGDIGAVAL DYPAGTSGSPILDKCGRVIGLYGNGVVIKNGSYVSAITQGRREEETPVECFEPSMLKKKQLTVLDLHPGAGKTR- RVLPEIVREAIKTRLR TVILAPTRVVAAEMEEALRGLPVRYMTTAVNVTHSGTEIVDLMCHATFTSRLLQPIRVPNYNLYIMDEAHFTDP- SSIAARGYISTRVE MGEAAAIFMTATPPGTRDAFPDSNSPIMDTEVEVPERAWSSGFDWVTDHSGKTVWFVPSVRNGNEIAACLTKAG- KRVIQLSRKTF ETEFQKTKHQEWDFVVTTDISEMGANFKADRVIDSRRCLKPVILDGERVILAGPMPVTHASAAQRRGRIGRNPN- KPGDEYLYGGGC AETDEDHAHWLEARMLLDNIYLQDGLIASLYRPEADKVAAIEGEFKLRTEQRKTFVELMKRGDLPVWLAYQVAS- AGITYTDRRWCF DGTTNNTIMEDSVPAEVWTRHGEKRVLKPRWMDARVCSDHAALKSFKEFAAGKRGAAFGVMEALGTLPGHMTER- FQEAIDNLA VLMRAETGSRPYKAAAAQLPETLETIMLLGLLGTVSLGIFFVLMRNKGIGKMGFGMVTLGASAWLMWLSEIEPA- RIACVLIVVFLLLV VLIPEPEKQRSPQDNQMAIIIMVAVGLLGLITANELGWLERTKSDLSHLMGRREEGATIGFSMDIDLRPASAWA- IYAALTTFITPAVQ HAVTTSYNNYSLMAMATQAGVLFGMGKGMPFYAWDFGVPLLMIGCYSQLTPLTLIVAIILLVAHYMYLIPGLQA- AAARAAQKRTA AGIMKNPVVDGIVVTDIDTMTIDPQVEKKMGQVLLIAVAVSSAILSRTAWGWGEAGALITAATSTLWEGSPNKY- WNSSTATSLCNI FRGSYLAGASLIYTVTRNAGLVKRRGGGTGETLGEKWKARLNQMSALEFYSYKKSGITEVCREEARRALKDGVA- TGGHAVSRGSAKL RWLVERGYLQPYGKVIDLGCGRGGWSYYAATIRKVQEVKGYTKGGPGHEEPMLVQSYGWNIVRLKSGVDVFHMA- AEPCDTLLCDI GESSSSPEVEEARTLRVLSMVGDWLEKRPGAFCIKVLCPYTSTMMETLERLQRRYGGGLVRVPLSRNSTHEMYW- VSGAKSNTIKSVS TTSQLLLGRMDGPRRPVKYEEDVNLGSGTRAVVSCAEAPNMKIIGNRIERIRSEHAETWFFDENHPYRTWAYHG- SYEAPTQGSASS LINGVVRLLSKPWDVVTGVTGIAMTDTTPYGQQRVFKEKVDTRVPDPQEGTRQVMSMVSSWLWKELGKHKRPRV- CTKEEFINKV RSNAALGAIFEEEKEWKTAVEAVNDPRFWALVDKEREHHLRGECQSCVYNMMGKREKKQGEFGKAKGSRAIWYM- WLGARFLEF EALGFLNEDHWMGRENSGGGVEGLGLQRLGYVLEEMSRIPGGRMYADDTAGWDTRISRFDLENEALITNQMEKG- HRALALAIIKY TYQNKVVKVLRPAEKGKTVMDIISRQDQRGSGQVVTYALNTFTNLVVQLIRNMEAEEVLEMQDLWLLRRSEKVT- NWLQSNGWDR LKRMAVSGDDCVVKPIDDRFAHALRFLNDMGKVRKDTQEWKPSTGWDNWEEVPFCSHHFNKLHLKDGRSIVVPC- RHQDELIGRA RVSPGAGWSIRETACLAKSYAQMWQLLYFHRRDLRLMANAICSSVPVDWVPTGRTTWSIHGKGEWMTTEDMLVV- WNRVWIEE NDHMEDKTPVTKWTDIPYLGKREDLWCGSLIGHRPRTTWAENIKNTVNMVRRIIGDEEKYMDYLSTQVRYLGEE- GSTPGVL 9320_Zika_PF_1F SEQ ID NO: 80 ttaggatccGTTGTTGATCTGTGTGAAT 9321_Zika_PF_1R

SEQ ID NO: 81 taactcgagCGTACACAACCCAAGTT 9322_Zika_PF_2F SEQ ID NO: 82 ttaggatccTCACTAGACGTGGGAGTG 9323_Zika_PF_2R SEQ ID NO: 83 taactcgagAAGCCATGTCYGATATTGAT 9324_Zika_PF_3F SEQ ID NO: 84 ttaggatccGCATACAGCATCAGGTG 9325_Zika_PF_3R SEQ ID NO: 85 taactcgagTGTGGAGTTCCGGTGTCT 9326_Zika_PF_4F SEQ ID NO: 86 ttaggatccGAATAGAGCGAARGTTGAGATA 9327_Zika_PF_4R SEQ ID NO: 87 taactcgAGTGGTGGGTGATCTTCTTCT 9328_Zika_PF_5F SEQ ID NO: 88 ttaggatcCAGTCACAGTGGAGGTACAGTAC 9329_Zika_PF_5R SEQ ID NO: 89 taactcgagCRCAGATACCATCTTCCC 9330_Zika_PF_6F SEQ ID NO: 90 ttaggatCCCTTATGTGCTTGGCCTTAG 9331_Zika_PF_6R SEQ ID NO: 91 taactcgagTCTTCAGCCTCCATGTG 9332_Zika_PF_7F SEQ ID NO: 92 ttaggatccAATGCCCACTCAAACATAGA 9333_Zika_PF_7R SEQ ID NO: 93 taactcgagTCATTCTCTTCTTCAGCCCTT 9334_Zika_PF_8F SEQ ID NO: 94 ttaggatccAAGGGTGATCGAGGAAT 9335_Zika_PF_8R SEQ ID NO: 95 taactcgagTTCCCTTCAGAGAGAGGAGC 9336_Zika_PF_9F SEQ ID NO: 96 ttaggatccTCTTTTGCAAACTGCGATC 9337_Zika_PF_9R SEQ ID NO: 97 taactcgagTCCAGCTGCAAAGGGTAT 9338_Zika_PF_10F SEQ ID NO: 98 ttaggatccGTGTGGACATGTACATTGA 9339_Zika_PF_10R SEQ ID NO: 99 taactcgagCCCATTGCCATAAAGTC 9340_Zika_PF_11F SEQ ID NO: 100 ttaggatccTCATACTGTGGTCCATGGA 9341_Zika_PF_11R SEQ ID NO: 101 taactcgagGCCCATCTCAACCCTTG 9342_Zika_PF_12F SEQ ID NO: 102 ttaggatccTAGAGGGCTTCCAGTGC 9343_Zika_PF_12R SEQ ID NO: 103 taactcgAGATACTCATCTCCAGGTTTGTTG 9344_Zika_PF_13F SEQ ID NO: 104 ttaggatccGAAAACAAAACATCAAGAGTG 9345_Zika_PF_13R SEQ ID NO: 105 taactcgagGAATCTCTCTGTCATGTGTCCT 9346_Zika_PF_14F SEQ ID NO: 106 ttaggatccTTGATGGCACGACCAAC 9347_Zika_PF_14R SEQ ID NO: 107 ttaggatccGTTGTTGATCTGTGTGAAT 9348_Zika_PF_15F SEQ ID NO: 108 taactcgagCAGGTCAATGTCCATTG 9349_Zika_PF_15R SEQ ID NO: 109 ttaggatccTGTTGTGTTCCTATTGCTGGT 9350_Zika_PF_16F SEQ ID NO: 110 taactcgaGTGATCAGRGCCCCAGC 9351_Zika_PF_16R SEQ ID NO: 111 ttaggatccTGCTGCCCAGAAGAGAA 9352_Zika_PF_17F SEQ ID NO: 112 taactcgaGCACCAACAYGGGTTCTT 9353_Zika_PF_17R SEQ ID NO: 113 ttaggatcCTCAAGGACGGTGTGGC 9354_Zika_PF_18F SEQ ID NO: 114 taactcgagCAATGATCTTCATGTTGGG 9355_Zika_PF_18R SEQ ID NO: 115 ttaggatccTATGGGGGAGGACTGGT 9356_Zika_PF_19F SEQ ID NO: 116 taactcGAGCCCAGAACCTTGGATC 9357_Zika_PF_19R SEQ ID NO: 117 ttaggatcCAGACCCCCAAGAAGGC 9358_Zika_PF_20F SEQ ID NO: 118 taactcgagCCCCTTTGGTCTTGTCT 9359_Zika_PF_20R SEQ ID NO: 119 ttaggatccAGGAAGGATGTATGCAGATG 9360_Zika_PF_21F SEQ ID NO: 120 taactcgagACATTTGCGCATATGATTTTG 9361_Zika_PF_21R SEQ ID NO: 121 ttaggatccAGGAAGGACACACAAGAGT 9362_Zika_PF_22F SEQ ID NO: 122 taactcgagACAGGCTGCACAGCTTT 9363_Zika_PF_22R SEQ ID NO: 123 ttaggatccTCTCTCATAGGGCACAGAC

In some embodiments, the Zika virus has a polyprotein, including an envelope (E) protein, with an amino acid sequence provided by any one of SEQ ID NO: 14-69 or 78. In some embodiments, the polyprotein or E protein sequence is at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%. 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% identical to any one of SEQ ID NOs: 2-69 or 78.

The terms "identical" or percent "identity" in the context of two or more nucleic acids or amino acid sequences refer to two or more sequences or subsequences that are the same. Two sequences are "substantially identical" if two sequences have a specified percentage of amino acid residues or nucleotides that are the same (e.g., at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% identity) over a specified region or over the entire sequence, when compared and aligned for maximum correspondence over a comparison window, or designated region as measured using one of the following sequence comparison algorithms or by manual alignment and visual inspection. Optionally, the identity exists over a region that is at least about 50 nucleotides (or 10 amino acids) in length, or more preferably over a region that is 100 to 500 or 1000 or more nucleotides (or 20, 50, 200 or more amino acids) in length. In some embodiments, the identity exists over the length of a protein, such as the E protein.

For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. Methods of alignment of sequences for comparison are well known in the art. See, e.g., by the local homology algorithm of Smith and Waterman (1970) Adv. Appl. Math. 2:482c, by the homology alignment algorithm of Needleman and Wunsch, J. Mol. Biol. 48:443, 1970, by the search for similarity method of Pearson and Lipman. Proc. Natl. Acad. Sci. USA 85:2444, 1988, by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, Jalview and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group. 575 Science Dr., Madison. Wis.), by multi sequence alignment implementation using e.g. CLUSTALW (Larkin et al., (2007). Bioinformatics, 23, 2947-2948.) or MAFFT (Katoh & Toh 2008 Briefings in Bioinformatics 9:286-298), or by manual alignment and visual inspection (see. e.g., Brent et al., Current Protocols in Molecular Biology, John Wiley & Sons, Inc. (Ringbou ed., 2003)). Two examples of algorithms that are suitable for determining percent sequence identity and sequence similarity are the BLAST and BLAST 2.0 algorithms, which are described in Altschul et al., Nuc. Acids Res. 25:3389-3402, 1977 and Altschul et al., J. Mol. Biol. 215:403-410, 1990, respectively.

EXAMPLES

Example 1: Development of a Purification Process for Live Attenuated Chikungunya Virus Vaccine Produced in Vero Cells

A downstream process was developed for the purification of infectious Chikungunya virus particles whereby non-infectious virus particles and aggregates are removed by the addition of protamine sulphate. The unexpected and novel purification properties of protamine sulphate (PS) were evaluated in purification processes for Chikungunya Virus (ChikV) as follows:

A downstream purification process for the attenuated Chikungunya virus mutant ".DELTA.5nsP3" (as described by Hallengird et al., 2014, supra and provided by SEQ ID NO: 77) produced under standard cell culture conditions in Vero cells was developed. The attenuated .DELTA.5nsP3 Chikungunya virus was derived from the strain LR2006-OPY1, the complete genome of which is provided herein as SEQ ID NO: 72. Briefly, the downstream process consists of crude harvest filtration followed by concentration and diafiltration on a tangential flow filtration (TFF) system. Host cell DNA and host cell proteins were reduced by precipitation with protamine sulphate and by batch adsorption, respectively. Sucrose density gradient centrifugation was done as a final polishing step. Out of 16 roller bottles 1.times.10.sup.12 total PFU were purified with an overall DSP process yield of 10-15% (.about.1 log 10 TCID50 loss). Sucrose gradient pool samples were characterized with regard to product-related impurities, such as hcDNA, HCP and endotoxins and met safety criteria.

Harvest of Vero Cell Culture Medium Containing ChikV .DELTA.5nsP3

ChikV .DELTA.5nsP3 was grown on Vero cells in roller bottles. A first harvest was performed after 24 hours post infection (hpi; day 1 harvest) and stored at 2-8.degree. C. until further processing. After the first harvest, fresh medium was added and the roller bottles were returned to the incubator. A second harvest was done after 48 hours post infection (day 2 harvest) and stored at 2-8.degree. C.

Filtration of Crude Cell Culture Harvest

At both harvest timepoints, the crude harvest was immediately filtered using a 0.2 .mu.m filter capsule (GE ULTA.TM. CG, 2 inch). The filtered harvest after 48 hpi was pooled together with the 24 hpi harvest and the pooled filtered harvest material was immediately further processed by ultrafiltration.

Purification of ChikV .DELTA.5nsP3 by Tangential Flow Filtration (TFF)

The pooled filtered harvest material was further processed by tangential flow filtration (TFF) in order to concentrate the harvest, reduce host cell proteins and replace the depleted cell culture medium with a defined buffer system (buffer exchange). A Millipore TFF system (Millipore Pellicon II mini membrane holder) equipped with a 100 kDa cutoff PES membrane module (Pellicon2 Biomax, 1000 cm.sup.2) was used for concentration and buffer exchange. A Pellicon2 Biomax membrane module was mounted on the Pellicon II mini filter holder and the device was connected to a peristaltic pump. The system was first rinsed with ultra-pure water and then sanitized by recirculation of 0.1 M NaOH for 60 min. In case the system was not used immediately, it was stored in 0.1 M NaOH until use. Prior to use the system was rinsed with 1 L of RO-water followed by buffer A until the permeate pH value was constant at pH 7.4.+-.0.2.

Adjustment of the ChikV .DELTA.5nsP3 Harvest (pH, Salt)

The pooled filtered harvest material was adjusted to a final concentration of 25 mM Tris and 150 mM NaCl using stock solutions of both components (see Table 1). This adjustment was done to increase buffering capacity and to reduce unspecific adsorption to the membrane. The necessary volumes of stock solutions D (1 M Tris, pH 7.4) and E (4.5 M NaCl) were calculated as follows: Volume of stock solution D (1 M Tris, pH 7.4) added to pooled harvest=Volume of pooled filtered harvest/40 Volume of stock solution E (4.5 M NaCl) added to pooled harvest=Volume of pooled filtered harvest/30 Example: 4 L harvest obtained from 20 RB (850 cm.sup.2) would require addition of 100 mL stock solution D (1 M Tris, pH 7.4) and 133 mL stock solution E (4.5 M NaCl).

The calculated volumes of stock solution D and Buffer E were added to the pooled filtered harvest under gentle stirring. The adjusted harvest was then stirred using a magnetic stirrer for 5 minutes at room temperature.

Concentration and Diafiltration of the ChikV .DELTA.5nsP3 Harvest by TFF

In a first step, the adjusted harvest material was concentrated approximately 10 fold. The feed flowrate was approximately 220 mL/min. The transmembrane flux at a transmembrane pressure of approximately 0.6 bar was in the range of 90.+-.5 mL/min per 1000 cm.sup.2 membrane. After concentration, the cell culture medium was exchanged against 25 mM Tris, 150 mM NaCl, pH 7.5, by continuous diafiltration with 6 volume exchanges. The diafiltration buffer was supplied to the feed vessel from a measuring cylinder by a second peristaltic pump set to a flowrate of approximately 90 mL/min. Minor flowrate adjustments of the second peristaltic pump in the range of +10 mL/min were done manually to ensure a constant volume of harvest in the feed vessel. After 6 volume exchanges, diafiltration was stopped. The liquid remaining in the membrane module was recovered by pumping the module empty with air.

Sucrose Addition to Diafiltrated ChikV .DELTA.5nsP3 Material

After diafiltration, sucrose stock solution H (50% (w/w) sucrose solution) was added to the diafiltrated material to achieve a final sucrose concentration of 10% (w/w). The volume of buffer H was calculated as follows: Volume of stock solution H added (mL)=Volume (mL) of diafiltrated ChikV material.times.0.25 (dilution factor=1:4) (i.e., final sucrose concentration is 10%) Example: 400 mL diafiltrated ChikV solution would require addition of 100 mL stock solution H (50% sucrose).

The calculated volume of solution H was added to the diafiltrated ChikV .DELTA.5nsP3 material under gentle stirring and the solution was then stirred using a magnetic stirrer for a further 5 minutes at room temperature. (At this stage of the process the material can be either immediately further processed or stored frozen (<-65.degree. C., hold step).)

DNA Reduction by Protamine Sulphate Precipitation

A DNA precipitation step using protamine sulphate (PS) was performed to reduce hcDNA. Protamine sulphate stock solution L (50 mg/mL PS in PBS) was added to the diafiltrated ChikV .DELTA.5nsP3 material to a final nominal concentration of .about.1.6 mg/mL. The necessary volume of stock solution L was calculated as follows: Volume of stock solution L (50 mg/mL PS) added=Volume of diafiltrated ChikV .DELTA.5nsP3 material in 10% sucrose/31 Example: 500 mL diafiltrated ChikV .DELTA.5nsP3 solution in 10% sucrose would require addition of 16 mL stock solution L (50 mg/mL PS in PBS).

The protamine sulphate stock solution was added while stirring the ChikV .DELTA.5nsP3 material using a magnetic stirrer followed by incubation at 2-8.degree. C. for 30 minutes. After incubation, the precipitate was not removed. The material was immediately further processed by batch adsorption with Capto.TM. Core 700 chromatography media.

Batch Adsorption with Capto.TM. Core 700

To reduce HCPs, a batch adsorption step with Capto.TM. Core 700 (CC700) chromatography medium was performed after DNA precipitation. CC700 slurry (50% slurry in buffer A) was added directly to the protamine sulphate treated material. The required slurry volume was determined based on the volume of .DELTA.5nsP3 ChikV harvest material (d1+d2) and was calculated as follows: Volume of CC700 slurry added to PS-treated concentrated harvest (mL)=Volume of .DELTA.5nsP3 ChikV harvest material (mL).times.0.02 (dilution factor=1:50) (i.e., final concentration of CC700 is 1%) After slurry addition, the material was incubated at 4.degree. C. for 15 minutes under constant agitation using a magnetic stirrer. After incubation, the CC700 solid matter was allowed to settle by gravity for 10 minutes. The .DELTA.5nsP3 ChikV material was then removed from the top of the solution in order to avoid blocking of the filter by the CaptoCore particles. The remaining CaptoCore particles and the DNA precipitate were then removed from the solution by filtration using a 0.2 .mu.m Mini Kleenpak EKV filter capsule (Pall). The resulting filtrate was further processed by sucrose density gradient centrifugation. Sucrose Density Gradient Centrifugation

Sucrose density gradient centrifugation (SGC) was used for final concentration and polishing of the .DELTA.5nsP3 ChikV material. The .DELTA.5nsP3 ChikV material was loaded on top of a solution consisting of three layers of sucrose with different densities. The three sucrose layers were selected based on a preliminary study which showed the formation of a linear sucrose gradient and good separation of the virus particles from residual contaminants. The optimal volumes of the sucrose solutions were determined empirically. The volumes of individual layers for a centrifugation at 500 mL scale are shown in Table 3.

TABLE-US-00008 TABLE 3 Sucrose concentrations and volumes (500 mL scale). Volume Solution (mL) Harvest with 10% sucrose 360 15% sucrose 40 35% sucrose 40 50% sucrose 60 Total volume 500

Preparation of the Sucrose Gradient

The sucrose gradient bottles (500 mL) were prepared by underlaying the individual sucrose layers. A 3.5 mm ID plastic tube was attached to 60 cm of peristaltic pump tubing. The plastic tube was mounted on a laboratory stand using a clamp and placed into the centrifuge bottle. The nozzle of the plastic tube was placed at the bottom of the bottle. Using a peristaltic pump set to a flow rate of 25 mL per minute, the .DELTA.5nsP3 ChikV material and the sucrose solutions were pumped into the cylinder. A measuring cylinder was used as a feed vessel. The first solution pumped was the .DELTA.5nsP3 ChikV material as it had the lowest density (10% sucrose (w/w)). Following the addition of the .DELTA.5nsP3 ChikV material, the sucrose solutions were pumped in ascending order starting with the lowest (15%), followed by the 35% sucrose solution and finishing with the highest density sucrose solution (50%). After all sucrose solutions were transferred, the plastic tubing was carefully removed in order not to disturb the layers. An illustration of a completed gradient is shown in FIG. 14.

Centrifugation

Prior to centrifugation a Beckman Avanti JXN-26 centrifuge equipped with rotor Beckman 10.500 was pre-cooled to 4.degree. C. The prepared SG bottles were carefully transferred into the pre-cooled (4.degree. C.) rotor so as to not to disturb the sucrose layers. The bottles were centrifuged at 10,000 rpm (.about.18,500 rcf) at 4.degree. C. for 17-20 hours. (In case a different centrifuge system with a different rotor would be used, the necessary speed and centrifugation times would need to be calculated based on the k-factor in order to achieve comparable centrifugation efficiency.)

Sucrose Gradient Harvest Harvesting of the sucrose gradient following centrifugation was done manually using a peristaltic pump.

A 3.5 mm ID plastic tube attached to 60 cm of peristaltic pump tubing was used for harvesting the sucrose gradient. The 500 mL bottle containing the centrifuged gradient was mounted onto a laboratory stand in a tilted position (.about.12.degree.) using a clamp. The plastic tubing was then placed into the bottle touching the bottom edge of the bottle and was fastened in position using a clamp. This resulted in a small gap of 1-2 mm between the tubing inlet and the bottom of the bottle (see FIG. 14). Using a peristaltic pump set to a flow rate of 60 mL per minute, the gradient was harvested and manually split into 5 mL fractions. A third of the bottle volume was harvested and the rest was discarded. The fractions were immediately tested by measuring UV absorbance in a plate reader as described below.

Analysis of fractions by UV absorbance and SEC-HPLC

UV absorbance measurement was used as primary method for analysis of the sucrose gradient fractions. Absorbance at 214, 280 and 260 nm was tested immediately after fractionation was completed. Briefly, a 100 .mu.L sample of each fraction was transferred into a 96 well plate and absorbance at 214, 260 and 280 nm was measured using a plate reader. The absorbance values were plotted against the fraction number. A representative profile is shown in FIG. 11A. The .DELTA.5nsP3 ChikV containing fractions were indicated by a peak in all three measured wavelengths (FIG. 11A, grey shaded area). The presence of impurities was indicated by an increase of the UV214 signal after the main peak. The fractions comprising the main peak were pooled from the peak start to the valley of the 214 nm curve. This method can be used as single method for pooling .DELTA.5nsP3 ChikV fractions.

After identification of the virus containing fractions, the respective fractions were pooled. Pooling criteria for SGC fractions were based on UV 260 nm data, e.g. start of pooling at .about.10% of peak maximum, end of pooling at .about.30% of peak maximum. (Final pooling criteria at a manufacturing scale may need to be determined empirically.) The sucrose gradient pool was either stored at <-65.degree. C. or immediately further formulated to drug substance (DS).

Size Exclusion Chromatography

The final pooled SGC fractions containing purified infectious .DELTA.5nsP3 ChikV particles were analyzed for purity by SEC-HPLC. In brief, SEC was performed as follows: a Superose 6 10/300 Increase column (GE Healthcare) equilibrated with PBS+250 mM NaCl, pH 7.4 at 1 ml/min and 25.degree. C., was used to detect ChikV particles at 214 nm detection wavelength in the pooled samples. SEC-HPLC is a semi-quantitative (relative yield) and qualitative (purity) method that separates intact virus particles from virus aggregates and host cell proteins (HCPs). The method cannot distinguish between infectious and non-infectious virus particles due to their identical retention time.

As shown in FIG. 11B, there were two defined peaks identified by SEC: the .DELTA.5nsP3 ChikV peak and a peak corresponding to buffer components. The SGC step yield based on SEC-HPLC data for pooled fractions F6-F11 was estimated at .about.70%. The final purity of the .DELTA.5nsP3 ChikV SGC pool, based on SEC-HPLC analysis, was estimated at >95%.

SDS-PAGE and Silver Stain

SDS-PAGE silver stain was performed in order to qualitatively assess sample purity throughout the purification process from the first crude harvest through SGC. Briefly, ChikV process samples analyzed by SDS-PAGE/silver stain were diluted 1:1.33 with LDS buffer and were heated to 70.degree. C. for 5 minutes. The samples were loaded onto 4-12% Bis-Tris Gels (NuPAGE). Silver staining was done using the Silver Express staining kit (Invitrogen).

A silver-stained gel of a representative ChikV .DELTA.5nsP3 purification is shown in FIG. 11C. The viral proteins E1, E2 and C are marked on the right-hand side of the gel. The final SGC pool (fraction 7-fraction 11) is shown in lane 12. Note that a defined HCP band migrating between ChikV protein E2 and C still appears after CaptoCore700 treatment that has been identified as a single band in SDS-PAGE. This impurity is removed by sucrose gradient centrifugation, but can still be seen in fractions 13 and 14 (corresponding to lanes 14 and 15 of FIG. 11C).

Enrichment of Infectious .DELTA.5nsP3 ChikV particles by PS Treatment

Although generally used as a method of removing contaminating hcDNA, it was observed in the course of the present invention that PS treatment also removes virus aggregates and HCPs. Size exclusion chromatography (SEC-HPLC, as described above) was used throughout the purification process to determine the purity of the ChikV virus relative to impurities which also generate UV absorption.

As can be seen in FIG. 12B, treatment with PS reduces not only host cell proteins and low molecular weight contaminants of the .DELTA.5nsP3 ChikV preparation, but also reduces the SEC area corresponding to virus products, including aggregates as indicated. A surprising finding, however, was that even a reduction of the total SEC area by 86% (in a representative experiment shown in FIG. 12A, grey portion of bars) did not result in a concomitant reduction in infectious virus particles as measured by TCID50 (FIG. 12A, left axis). Instead, even though a large percentage of virus particles were removed by PS treatment, the majority of infectious particles remained. This observation indicates that PS treatment selectively enriches infectious virus particles from a larger pool of total virus particles present in the crude harvest.

TCID50 was performed to quantify infectious virus particles during the course of the purification process and to assign an active virus titer to final drug substance and drug product samples. Briefly, Vero cells were seeded at 2.times.10.sup.4 cells per well in 100 .mu.L medium (EMEM with 2 mM L-Glutamine+5% FBS+1% antibiotic/antimycotic) in 96-well TC-treated flat-bottom plates and incubated overnight at 35.degree. C./5% CO.sub.2. On day two, Vero cell monolayers were infected by adding 100 .mu.L of 1:10 serial dilutions of test samples to each of quintuplicate wells seeded with Vero cells and incubated at 35.degree. C./5% CO.sub.2. On day seven, plaques were counted by visualization under a microscope. The TCID50 was calculated according to the Reed & Munch endpoint calculation method (Reed, L. J.; Muench, H. (1938) A simple method of estimating fifty percent endpoints, The American Journal of Hygiene 27: 493-497).

Furthermore, electron microscopy of .DELTA.5nsP3 ChikV samples before and after PS treatment showed that not only large aggregates but also smaller non-infectious virus-like particles (essentially not fully assembled particles lacking the RNA genome) were effectively removed by PS (FIG. 13).

This enrichment of infectious virus particles was also observed when analyzing day one and day two crude harvests separately. As presented in Table 4, the SEC area (total virus particles) of the day 1 harvest remains roughly the same after PS treatment; whereas a large decrease in virus peak area is seen for the day 2 harvest after PS treatment. This observation was confirmed by MALLS analysis of the virus preparation, wherein it was seen that a higher percentage of virus particles were of the correct size following PS treatment. Similarly to the results shown in FIG. 12, day 1 and day 2 harvests showed no reduction in infectious particles as measured by TCID50 following PS treatment, indicating that mainly non-infectious, immature and/or aggregated virus particles are removed during the PS treatment and infectious particles are enriched in the preparation.

The PS-treated samples were further purified by sucrose gradient centrifugation (see FIG. 14 for a schematic preparation of an optimized sucrose gradient). An optimal sucrose gradient was determined experimentally as shown in FIG. 15. Results of the further purification of PS-treated ChikV on the optimized sucrose gradient of the invention are shown in FIG. 15D.

TABLE-US-00009 TABLE 4 Overview of the process of .DELTA.5nsP3 ChikV purification as described in Example 1. SEC-MALLS analysis of harvests before and after PS treatment shows the removal of larger virus particles (aggregates), an effect that is particularly pronounced for day 2 harvests. MALLS Infectious SEC % correct particles Area Total size TCID50 [mAU*min] particles/mL (20-40 nm) log 10 Harvest 1 (H1) 57 1.17E+11 49% 10.2 H1 + protamine 53 1.33E+11 81% 10.0 sulphate Harvest 2 (H2) 36 4.60E+09 3% 7.9 H2 + protamine 2 8.80E+09 59% 7.9 sulphate Combined 67 2.60E+10 14% 9.9 Harvests (C) C + protamine 24 8.00E+10 72% 10.1 sulphate

Finally, an overview of the relative amounts of .DELTA.5nsP3 ChikV particles and other components as measured by SEC-HPLC at various steps throughout the entire virus purification process from crude harvest (a) to the final SGC purified pool is presented in FIG. 16. In sum, not only are the vast majority of contaminants and undesired products removed by the process, infectious ChikV particles are highly purified. As shown by the previously presented data, the final preparation is a highly enriched preparation of infectious ChikV particles.

Drug Substance (DS) Formulation

The pooled SGC fractions are diluted with DS formulation buffer M (10 mM Tris, 5% Sucrose (w/w), 1% (10 mg/mL) rHSA, pH 7.4.+-.0.2). The final target volume of DS should be in the range of approximately 2 L. Based on current data the estimated range of the dilution factor might be 1:20 to 1:50.

Final DS Sterile Filtration

The final DS was filtered under aseptic conditions in a laminar flow hood using a sterility grade 0.2 .mu.m syringe filter (e.g. 0.2 .mu.m Mini Kleenpak EKV filter capsule with 220 cm.sup.2 filter surface, Pall).

Quantification of Host Cell DNA (hcDNA) Host Cell Protein (HCP) and Endotoxin

The residual host cell DNA content of the sucrose gradient pool samples was determined by using the qPCR based assay. The DNA content in SGC pool was determined to be <0.002 ng/mL. The presence of residual host cell protein (HCP) from Vero cells was determined by ELISA. Residual host cell proteins present in the sucrose gradient pool samples were quantified using the Vero Cell HCP ELISA kit (Cygnus, F500). The residual host cell protein content in SGC pool was determined to be <200 ng/mL.

Endotoxin content of the SGC pool and DS was measured by Endosafe.RTM.-PTS.TM. system (Charles River). The system uses Limulus Amembocyte Lysate (LAL) reagents by a kinetic chromogenic methodology to measure color intensity directly related to the endotoxin content in a sample. Each cartridge contains precise amounts of a licensed LAL reagent, chromogenic substrate and an endotoxin control standard. Samples were diluted 1:100 in WFI. The SGC Pool F7-F11 was determined to be <5.00 EU/mL; likewise, the Drug Substance was also determined to have <5.00 EU/mL.

The following specifications for impurities in final Drug product were proposed: hcDNA <10 ng/dose; Endotoxins <50 EU/dose; HCP <200 ng/dose. These residual specifications would already be met in the highly concentrated SGC pool (.about.10 log TCID50/mL), which provides a high margin of safety considering the high dilution factor of SGC pool to final DP of >1:1000.

Example 2: Production of a Zika Drug Substance Suitable for Application as a Vaccine in Humans and Animals

Materials and Methods:

For the production of ZikaV the JEV process platform (Srivastava et al., Vaccine 19 (2001) 4557-4565; U.S. Pat. No. 6,309,650B1) was used as a basis. Small changes of certain process steps were adapted to ZikaV properties and to improve purity. A short summary of the process steps is outlined below (see also FIGS. 17A and B). Briefly, the unexpected and novel purification properties of protamine sulphate (PS) were evaluated in purification processes for Zika Virus similarly as found above. Again non-infectious virus particle aggregates, HCP and other LMW impurities were removed by PS precipitation as shown by removal of aggregate shoulder in SEC-HPLC and no loss of infectious virus titer by PS treatment (FIG. 18). Further optimization of the Zika purification protocol is provided below.

Upstream:

Roller Bottle based Vero cell expansion (25.times.850 cm2 CellBind): 5% CO.sub.2, 35.degree. C., MEM+2 mM L-Glutamine+10% FBS Infection with ZikaV research Master Seed Bank (rMSB) at MOI 0.01 Virus Production without serum 5% CO.sub.2, 35.degree. C., MEM+2 mM L-Glutamine Multiple harvests (days 2, 3, 5 and 7) with re-feed Sterile filtration of harvests and storage at 2-8.degree. C. until further processing Downstream: Pooling of harvests and concentration by ultrafiltration (100 kDa) Stabilization of concentrated harvest (Tris/10% sucrose) for storage if required (-80.degree. C.) Removal of hcDNA by Protamine Sulphate (2 mg/mL) Sucrose Gradient Purification (optimized three layered gradient) Formaldehyde Inactivation (0.02%, 22.degree. C., 10 days), neutralization with Na-metabisulfite Dilution to DS antigen target content and formulation with Aluminium hydroxide (0.5 mg Al/mL)

Zika Virus Strain H/PF/2013 was originally isolated from a 51-year-old woman (accession number KJ776791.1, also SEQ ID NO: 13 herein) from French Polynesia. A sample was obtained from the European Virus Archive (EVAg; Ref-SKU: 001v-EVA1545). Based on this material, a research master seed bank (rMSB) was prepared on Vero cells as the cell substrate and the genomic sequence was checked by sequencing. Because the genomic sequence at the 5' and 3' flanking sequences of Zika virus strain H/PF/2013 was unknown, primers for sequencing were designed in those regions based on other Zika virus strains whereas the internal primers were designed from the published sequence (SEQ ID NOs: 80 to 123, see also Table A). The sequence obtained from the rMSB by use of these primers is provided by SEQ ID NO: 78. There was 100% overlap of the sequence with the published sequence of Zika Virus Strain H/PF/2013 (SEQ ID NO: 13). However, we sequenced additional regions 5' (an additional 40 bp) and 3 (an additional 160 bp) represented in SEQ ID NO: 78. In a preferred embodiment, the Zika virus of the invention comprises SEQ ID NO: 78. The genomic RNA is somewhat longer than the sequence according to SEQ ID NO: 78 (perhaps an additional 200 bp). Additionally, a Zika virus adapted to a host cell such as e.g. Vero cells may be expected to contain one or more mutations. For these reasons, the Zika virus of the current invention comprises the sequence of SEQ ID NO: 78 or, preferably, a sequence with at least 95%, 96%, 97%, 98%, or at least 99% sequence identity to the sequence provided by SEQ ID NO: 78. Furthermore, because the viral genome is likely to contain even further flanking regions to SEQ ID NO: 78; in one embodiment, the Zika virus of the invention contains the sequence of SEQ ID NO: 78 and optionally further comprises extensions at the 5' and/or 3' ends of at least 10, at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 110, at least 120 or at least 130 nucleotides. In a preferred embodiment, the Zika virus comprises at least the coding sequence for the entire polyprotein of Zika Virus Strain H/PF/2013 of the invention i.e. the amino acid sequence of SEQ ID NO: 79 or a polyprotein with at least 95%, 96%, 97%, 98%, or at least 99% sequence identity to the sequence provided by SEQ ID NO: 79. Furthermore, the Zika virus comprises at least the coding sequence for the E-protein of Zika Virus Strain H/PF/2013 of the invention SEQ ID NO: 47 or an E-protein thereof with at least 95%, 96%, 97%, 98%, or at least 99% sequence identity to the sequence provided by SEQ ID NO: 47.

Virus Growth on Vero Cells

Vero cells were grown in Eagle's minimal essential medium (EMEM) containing 10% fetal bovine serum (FBS). Roller bottle cultures of Vero cell monolayers were infected with Zika Virus Strain H/PF/2013 at a multiplicity of infection (moi) of 0.01 plaque forming units (pfu) per cell. After 2 hours of virus adsorption, the cultures were washed 3 times with PBS and fed with EMEM without FBS and incubated at +35.degree. C. with 5% CO.sub.2. Infected Vero cell cultures were incubated until the virus titer reaches a desired level.

The culture medium was harvested at days 2, 3, 5 and 7 and were pooled from those harvest days and then centrifuged in a standard centrifuge. The supernatants were then filtered. Virus culture supernatants were concentrated by TFF ultrafiltration to remove cell culture media components and to reduce batch volume.

Evaluation of Harvest Procedure

The current JEV harvest process has scheduled harvests on days 3, 5, 7 and 9 post infection. To mimic the JEV process roller bottles were infected with ZIKV bank P4-FBS at an MOI of 0.01 in infection medium (MEM with 2% FBS+2 mM L-glutamine) for 2 hours. After removing the inoculum the cells were washed twice with PBS and 200 mL production medium (MEM+2 mM L-glutamine) was added.

After taking a sample on day 2 the first virus harvest was conducted on day 3 after infection. At this point significantly higher CPE could be observed compared to cells where virus was removed on day 2. Plaque assay analysis showed that the viral titers on day 2 were in the same range as for the standard harvesting schedule. However, starting with the day 3 harvest, the observed titers were significantly lower correlating with the increased CPE observed compared to the standard harvest schedule. On day 5 post infection no more living cells could be observed at all and the experiment was terminated with a final day 5 harvest.

TABLE-US-00010 TABLE 5 The calculated titers per plaque assay are summarized in the list below. Log 10 PFU/mL sample day 2 7.02 harvest day 3 6.66 harvest day 5 6.26

This finding led to an optimized harvest schedule to better control of CPE and allow additional harvest day 5 and 7, see FIG. 23. For both harvest days the optimized ZikaV protocol yield significant higher virus titers compared to the modified protocol showing that the time of the first harvest is crucial for production yields. Additionally first harvesting at day 3 results in maximum 2 harvest points whereas first harvesting at day 2 allows for 4 harvest points further increasing the yield gain.

Downstream Purification of Zika virus

The purification process was carried out at room temperature (18-22.degree. C.) unless stated otherwise. Virus purification started with concentration of filtered combined harvest using 100 kDa cut-off TFF ultrafiltration modules to remove cell culture media components and reduce batch volume. After concentration, the pooled filtered harvest material was adjusted to a final concentration of 25 mM Tris pH 7.5 and 10% sucrose (w/w) using stock solution of both components (see FIG. 19 for SEC-HPLC of different harvests prior to PS treatment). This allowed for freezing the concentrated harvest at <-65.degree. C. if required.

Host cell DNA and protein reduction as well reduction of non-infectious virus aggregates in the concentrated material was achieved by precipitation with protamine sulphate (2 mg/mL) followed by sucrose density centrifugation (2-8.degree. C.) as final polishing step (see FIG. 20 for SEC-HPLC of different harvests post PS treatment). The purification process was designed to be completed within 2 working days with SGC starting on end of day 1 followed by fractionation and SDS-PAGE analysis on day 2. The sucrose gradient fractions were stored at 2-8.degree. C. during the SDS-PAGE analysis (Silver staining) to identify the pure fractions containing ZikaV (see FIG. 21). After pooling the relevant fractions, the pool was diluted and inactivated by Formalin. After pooling the relevant fractions of sucrose gradient centrifugation, the pool was diluted 1:3 in PBS and inactivated by Formalin (0.02% v/v, 200 ppm). Fractions were subjected to analysis by SDS-PAGE.

Effect of PS Treatment on Virus Recovery

Samples of individual 30.times. concentrated harvests days 2, 3, 5 and 7 were analysed before (FIG. 19) and after PS (FIG. 20) treatment by SEC-HPLC and plaque assay. SEC-HPLC was used for determination of relative total ZikaV content (active+inactive) expressed as peak area, whereas the rel. ZikaV peak purity is given as relative content of virus monomer population to total virus peak. Plaque assay states the content of total active virus particles in each sample. Experimental results are summarized in Table 1. The virus peak recovery by SEC-HPLC was only between 12 to 36% with peak purity after PS treatment in the range of >90% (no virus aggregates detected). The recovery of active virus particles by plaque assay was all >100% (130-700%, range within the variability of the assay) showing that no active virus particles were lost during PS treatment. These results show that during PS treatment only non-infective (immature and/or aggregated virus) particles were removed.

TABLE-US-00011 TABLE 6 ZikaV recovery by SEC-HPLC and plaque assay before and after PS treatment. SEC-HPLC Peak area mAU*min SEC rel. virus monomer Harvest 30x 30x + Recovery content after PS day conc PS (%) (%) Day 2 101.36 18.63 18 89% Day 3 144.51 17.48 12 90% Day 5 19.97 5.92 30 96% Day 7 68.80 24.43 36 99% Plaque Assay PFU/mL Plaque Harvest 30x 30x + Recovery day conc PS (%) Day 2 3E+08 5E+08 179 Day 3 2E+08 4E+08 193 Day 5 1E+08 9E+08 700 Day 7 3E+08 4E+08 132

Sucrose Gradient Centrifugation

The PS treated harvest was split in two parts and loaded on two centrifuge bottles.

Sucrose density gradient centrifugation (SGC) was used for final concentration and polishing of the ZikaV material. The ZikaV PS treated concentrated harvest was loaded on top of a solution consisting of three layers of sucrose with different densities. The three sucrose layers were selected based on a preliminary study which showed the formation of a linear sucrose gradient and complete separation of the virus particles from residual contaminants as demonstrated for ChikV (FIG. 15D). The optimal volumes of the sucrose solutions were determined empirically. The volumes of individual layers for a centrifugation in 100 mL bottle scale are shown in Table 2.

TABLE-US-00012 TABLE 7 Individual layers/volumes for a centrifugation in bottle. Volume Solution (mL) PS treated harvest in 10% sucrose (L) 40 15% sucrose (J) 15 35% sucrose (I) 15 50% sucrose (H) 20 Total volume 90

The sucrose gradient bottles were prepared by stratifying the individual sucrose layers. A plastic tube was attached to peristaltic pump tubing. The plastic tube was mounted on a laboratory stand using a clamp and placed into the centrifuge bottle. The nozzle of the plastic tube was touching the bottom of the bottle. Using a peristaltic pump the ZikaV material and the sucrose solutions were pumped into the cylinder. A measuring cylinder was used as feed vessel. The first solution pumped was the ZikaV material as it represented the solution of lowest density (10% sucrose (w/w)). After the ZikaV material the sucrose solutions were pumped in ascending order starting with the 15% (w/w) solution J, followed by 35% sucrose solution I and finishing with the highest density sucrose solution H (50% (w/w)). The described setup is shown in FIG. 14. After all sucrose solutions were transferred the plastic tubing was carefully removed in order not to disturb the layers.

Prior to centrifugation the centrifuge was pre-cooled to 4.degree. C. The prepared SG bottles were carefully transferred into the pre-cooled rotor. (Note: Sudden movement of the bottles during transfer to the rotor must be avoided in order not to disturb the sucrose layers.) The bottles were centrifuged at .about.11.000 RCF max at 4.degree. C. for at least 20 hours, no brake/deceleration activated. In case a different centrifuge system with a different rotor is used the necessary speed and centrifugation times need to be calculated based on the k-factor in order to achieve comparable centrifugation efficiency.

Harvesting of the sucrose gradient was done manually using a peristaltic pump. A plastic tube attached to peristaltic pump tubing was used for harvesting the sucrose gradient. The bottle containing the gradient was mounted onto a laboratory stand in a tilted position (.about.12.degree.) using a clamp. The plastic tubing was then placed into the bottle touching the bottom edge of the bottle and was fastened in position using a clamp.

This resulted in a small gap of 1-2 mm between the tubing inlet and the bottom of the bottle (see FIG. 14).

Using a peristaltic pump set to a flow rate of 30 mL per minute the gradient was harvested and manually split into 2 mL fractions. A total number of 32 fractions per bottle were harvested (.about.64 mL) and the remaining volume was discarded. The fractions were immediately tested by SDS-PAGE/silver stain to identify the virus containing fractions with sufficient high purity. Representative SDS-PAGE is shown in FIG. 21. Fraction 10-14 were pooled and further processed.

The purified viral solution was inactivated by incubation with 0.02% formaldehyde over a period of ten days in a 22.degree. C. controlled-temperature incubator. The formaldehyde is neutralized by addition of sodium metabisulphite on the tenth day.

The sucrose gradient pool (.about.17 mL after sampling) was further diluted 3-fold with PBS to a final volume of 51 mL in a PETG container. A volume of 1% formaldehyde (10,000 ppm) solution equivalent to 1/50 of the final volume of the pre-formaldehyde pool was added to this pool resulting in an effective concentration of 200 ppm. The formaldehyde-treated solution was mixed on a magnetic stirrer for 10 minutes. After sampling, the formaldehyde-treated viral solution was placed within a cooled incubator at 22.degree. C..+-.2.degree. C. On Day 5 post addition of formaldehyde, the formaldehyde-treated viral solution was filtered through a 0.2 m filter and then placed in the incubator at 22.degree. C..+-.2.degree. C. again. On Day 10, after removing the 10-Day inactivation final sample, a volume of 1% (of the weight of the final formaldehyde-treated viral solution) of 200 mM-sodium metabisulphite solution (2 mM final concentration) was aseptically transferred into the PETG container containing the formaldehyde-treated viral solution. After mixing for 5 minutes on a magnetic stirrer, the neutralized inactivated viral solution is held at room temperature (20 to 25.degree. C.) for a minimum of 30 minutes. After sampling, the neutralized inactivated viral solution is stored at 5.degree. C..+-.3.degree. C. until further processing.

Inactivation by Formaldehyde

Critical parameters for this step are final formalin concentration, temperature, mixing and transfer into a new container. A preliminary acceptance criterion for maximum pfu/mL (determined by plaque assay) has been set on the diluted pool pre formaldehyde treatment.

The quality of the neutralized inactivated viral solution was monitored by the following parameters: Plaque assay on Day 10, SEC-HPLC, SDS-PAGE/Western Blot.

Interestingly, SEC-HPLC analysis of samples taken during the inactivation period followed by neutralization with bisulfite showed more or less constant peak area throughout the inactivation period. This is in contrast to JEV where losses of viral particles up to 60% are observed using the process disclosed by Srivastava et al. Vaccine 19 (2001) 4557-4565. In a scale-down model the viral losses were even much higher due to surface/area ratio at smaller scale and high losses due to unspecific adsorption. Differences of the ZikaV inactivation experiment and JEV inactivation were noticed as follows: A) Much higher purity of ZikaV SGP pool with regard to residual PS (<2 .mu.g/mL) compared to JEV. The 3-fold ZikaV inactivated sample contained therefore <<1 .mu.g/mL of residual PS. Commercial JEV SGP pool contains on average .about.120 g/mL (up to 152 .mu.g/mL possible). The average dilution to inactivation solution of .about.14-fold results in a residual PS content up to .about.11 .mu.g/mL. It may be that higher amount of residual PS could cause virus precipitation due to cross-linking/reaction with formalin. B) ZikaV inactivation sample contained .about.10% sucrose (3-fold dilution of SGP pool containing .about.30-35% sucrose). Sucrose might have stabilizing effect of viral ZikaV particles during treatment with formalin. Dilution to DS and Formulation with Aluminium Hydroxide (DP)

For preparation of ZikaV drug substance used in mouse potency assay an antigen content (expressed as total viral particles or SEC peak area) of 5 times higher compared to Ixiaro was targeted. The basis for determination of antigen content was SEC-HPLC. Briefly, a Superose 6 10/300 Increase column (GE Healthcare) equilibrated with PBS+250 mM NaCl, pH 7.4 at 1 ml/min and 25.degree. C., was used to detect ZikaV at 214 nm detection wavelength in harvest samples and throughout the downstream process. In the current JEV process the antigen content in NIV is determined by a specific ELISA. A good correlation was observed between antigen content determined by ELISA and SEC-HPLC. On average, the antigen content in commercial NIV samples is in the range of 33 AU/mL corresponding to .about.5.2 mAU JEV peak area, see FIG. 22.

ZikaV NIV day 10 (Zika peak .about.36 mAU, analysed on Waters HPLC/Superose6 Increase column) was diluted with PBS to a target of 6.3 (.about.5.7.times. dilution). Aluminium hydroxide was added to a final concentration of 0.5 mg/mL Aluminium (1/20 v/v Alum 2% stock solution added) to prepare ZikaV Drug Product (DP). The DP was gently mixed for 5 min. An aliquot of the DP was removed, Alum sedimented by centrifugation and the clear supernatant analysed by SEC-HPLC. No ZikaV peak was detected in the supernatant indicating complete adsorption (estimated as >95%) of viral particles on the mineral adjuvant. Formulated ZikaV DP was stored at 2-8.degree. C.

The impurity profile of the inactivated Zika virus DS is comparable to the profile of JEV DS with the exception of a lower PS content (Table 8).

TABLE-US-00013 TABLE 8 Determination of impurity profile in Zika and JEV DS samples: Specification (JEV DS) JEV Zika HCP (ng/mL) <100 <LOQ <LOQ LOQ 12 ng/mL DNA (pg/mL) <200 <40 <40 LOQ 40 pg/mL Aggregates Not specified, <LOQ <LOQ by SEC- part of characterization MALLS (%) LOQ 5% PS (.mu.g/mL) Specification only at SGP pool to ~4* <<LOQ demonstrate consistent process performance (19-152 .mu.g/mL), *PS content in DS calculated based on PS content in SGP pool (~100 .mu.g/mL) and average dilution factor (~28.times.) to DS; LOQ 2 .mu.g/mL *Typical PS impurity in a JEV sample produced in accordance with protocol disclosed in Srivastava et al. Vaccine 19 (2001) 4557-4565.

SEC-MALLS Results

A representative SEC-HPLC elution profile of ZikaV NIV at 214 nm detection wave length is shown in FIG. 24. Note that BSA (50 g/mL) was added to the sample to minimize losses in HPLC glass vial due to unspecific surface adsorption. ZikaV monomer content was estimated as .about.98% with a multimer content of .about.2%.

SEC-MALLS analysis (FIG. 25) of the sample confirmed the radius Rz of the monomer ZikaV population peak 1 as 21.6 nm and .about.49 nm for the multimer peak 2. Cumulative particle size distribution showed that 89% of all viral particles are within a radius range between 18 to 25 nm (FIG. 26).

Results confirm purity and homogeneity of ZikaV NIV.

Viral Titer by Plaque Assay

TABLE-US-00014 TABLE 9 Active ZikaV pfus were quantified by plaque assay throughout the process. Sample Pfu/mL Harvest day 2 (filtered) 6.4 .times. 10.sup.7 Harvest day 3 (filtered) 1.0 .times. 10.sup.8 Harvest day 5 (filtered) 1.5 .times. 10.sup.8 Harvest day 7 (filtered) 1.1 .times. 10.sup.8 PS treated harvest 300.times. concentrate (=SGP load) 9.0 .times. 10.sup.8 SGP pool 8.9 .times. 10.sup.8 Inactivation start (SGP pool 1:3 diluted) 3.4 .times. 10.sup.8 Inactivation day 5 <LOD Inactivation day 10 <LOD

Comparison of PS and Benzonase on Process Performance

A direct comparison of DNA removal method of concentrated ZikaV harvest pool was done. One aliquot was treated with PS (2 mg/mL, 15 min at room temperature), the other aliquot was treated with Benzonase (50 U/mL, 2 mM MgCl2, 4 h RT, 48 h 2-8.degree. C.). Both samples were further purified by sucrose gradient as described in this report. Interestingly, the Benzonase treated samples did not yield any pure fractions after sucrose gradient centrifugation of the treated ZikaV harvest. In those fractions where the specific virus bands were detected, a high amount of host cell protein was detected throughout the collected fractions. The PS treated material resulted in pure ZikaV containing fractions as expected. This finding may suggest that PS is not only effective for DNA removal by precipitation; in addition it improves the recovery of virus particles in the gradient by disrupting interaction of DNA (fragments) and virus particles. Benzonase treatment does not remove DNA, it only results in its fragmentation. Residual DNA fragments might still interact with virus particles and residual HCPs resulting in cross-contamination and co-purification in the sucrose gradient. Pooled SGP fractions were also analysed by SEC-HPLC. Although a large peak was detected, SDS-PAGE confirmed that this sample was highly contaminated with HCPs. A large peak might be detected at UV214 and 280 nm after SEC-HPLC analysis due to possible interaction of HCPs with large virus particles, changing the UV absorbance.

Immunogenicity of Vero Grown Zika Virus

Immunization of Mice

Prior to immunization, groups of ten 6-week-old female CD1 mice were bled via vena facialis and pre-immune sera were prepared. One intraperitoneal immunizations of 200 .mu.L were administered. A dose titration (12 .mu.g, 3 .mu.g, 1 .mu.g, 0.33 .mu.g, 0.11 .mu.g, 0.037 .mu.g and 0.012 .mu.g, equivalent to the protein amount in IXIARO) of inactivated Zika virus formulated with aluminium hydroxide (Al(OH)3) at a final concentration of 0.7%. Three weeks after immunization, blood was collected and immune sera were prepared. All animal experiments were conducted in accordance with Austrian law (BGB1 Nr. 501/1989) and approved by "Magistratsabteilung 58".

Plaque Reduction Neutralization Test (PRNT)

Twelve well plates were used for PRNT. Each well was seeded with 1 mL medium containing 4.times.10.sup.5 Vero cells and incubated 35.degree. C. with 5% CO2 overnight. Pools of heat inactivated sera from each dose group were tested in triplicate. The target viruses (H/PF/2013 (SEQ ID NO: 13) or MR766 (SEQ ID NO: 11)) were diluted to 100 pfu/165 .mu.L. Equal volumes of target virus and serum dilution were incubated at 35.degree. C. with 5% CO.sub.2 for 1 hour. The cell culture medium was aspirated from the Vero cells and 330 .mu.L of the mixture target virus/serum dilution were added to each well and the plates were rocked back and forth 5 times before incubating for 2 hours at 35.degree. C. with 5% CO.sub.2. To each well 1 mL of a 2% methylcellulose solution containing EMEM and nutrients was added, the plates were then incubated for 5 days at 35.degree. C. with 5% CO.sub.2 before staining the cells for 1 hour with crystal violet/5% formaldehyde and subsequently washed 3 times with deionized water. The plates were air dried and the numbers of plaques in each well were manually counted.

Results

Neutralization was observed with serum pools from mice immunized with inactivated Zika virus vaccine (H/PF/2013) down to 37 ng (dosing equivalent to the amount protein in IXIARO.RTM.) against Zika viruses of both the Asian (H/PF/2013) and African (MR766) lineages (FIGS. 27 and 28, respectively). Complete inhibition was seen at the 1:20 serum dilution with an immunization dose down to 110 ng (dosing equivalent to the amount protein in IXIARO.RTM.). The neutralization of both the Asian (H/PF/2013) and African (MR766) lineages of the Zika virus was equivalent, which indicates high cross-neutralization between different Zika virus strains of the inactivated Zika virus vaccine (H/PF/2013).

Another neutralization assay was performed using the microneutralization assay as described by Larocca, et al. (2016, Nature doi:10.1038/nature18952). It was found that the inactivated Zika virus of the current invention had an MN50 (microneutralization) titer of 90 at 1 .mu.g of inactivated purified virus.

Further methods: The immunogenicity of inactivated Zika virus preparations is assessed using a mouse model of Zika infection. Groups of adult mice are immunized subcutaneously (s.c.) with 500, 50, or 5 ng of inactivated Zika virus with adjuvant (e.g. aluminium hydroxide with or without IC31.RTM.), or without adjuvant. An additional group of mice receive PBS as a negative control. Each group is administered the indicated inoculum at t=0 and in some cases also at three to four weeks later (t=3/4). Beginning approximately three weeks after administration of the last immunization, serum samples are obtained from each of the mice at regular intervals. The serum samples are tested for the presence of neutralizing antibodies using PRNT.

The in vivo protective efficacy of the inactivated Zika virus preparations is also assessed using a mouse model of Zika infection, i.e. IFN-alpha/beta receptor knock-out mice (A129) (see e.g. Dowall et al., 4. Mar. 2016, http://dx.doi.org/10.1101/042358) or blocking of the IFN-alpha/beta receptor by administration of anti-IFN-alpha/beta receptor monoclonal antibodies to C57BL/6 or BALB/c mice (see e.g. Pinto et al., 7. Dec. 2011, DOI: 10.1371/journal.ppat.1002407). For protection assays, groups of 10 three- to eight-weeks-old A129, C57BL/6 of BALB/c mice are inoculated subcutaneously in the hindquarters with inactivated Zika virus with adjuvant (aluminium hydroxide) or without adjuvant at t=0. Age-matched controls are inoculated with PBS or non-specific antigens in alum. Mice are optionally boosted with a second administration of the indicated inoculation three to four weeks later. The mice are then challenged subcutaneously at three to eight weeks post immunization by inoculation with a deadly dose of live Zika virus. One day prior to challenge of C57BL/6 and BALB/c mice, they are passively administered (intraperitoneally) anti-IFN-alpha/beta receptor monoclonal antibodies. Challenged mice are monitored daily for morbidity and mortality for up to twenty-one days. Another alternative is to challenge intracranially adult vaccinated/non-vaccinated adult mice and observe protection.

It is expected that the Zika virus produced by the process of the invention will provide very similar functional read-outs in in vitro, in vivo and finally human trials as the currently licensed JEV vaccine in the EU and US and elsewhere, IXIARO.RTM.. The dosage may alter but due to the very similar impurity profile and almost identical manufacture, a very similar efficacy and safety result will be expected as was determined for the currently licensed JEV vaccine (licensed in the EU and US and elsewhere).

Discussion & Conclusion

The existing manufacturing platform for production of inactivated JEV vaccine IXIARO.RTM. was used as a basis for a manufacturing feasibility study of inactivated ZikaV vaccine candidate (Asian strain H/PF/2013). The virus was produced on Vero cells cultivated in roller bottles. The virus was purified by PS treatment followed by an optimized sucrose gradient. Inactivation was done by formalin treat (0.02%, 10 days at 22.degree. C.). For exploratory immunization studies in mice, a DP formulated with Alum was prepared with an estimated 5-fold higher virus particle content compared to IXIARO.RTM., the commercial JEV Vaccine. The impurity profile of the DS met all criteria as defined in the specification for IXIARO.RTM., the commercial JEV vaccine. The neutralization of both the Asian (H/PF/2013) and African (MR766) lineages of the Zika virus was equivalent, which indicates high cross-neutralization between different Zika virus strains of the inactivated Zika virus vaccine (H/PF/2013).

The in vivo data regarding immunogenicity of the inactivated Zika virus vaccine of the current invention indicates that the virus is surprisingly potently immunogenic and also highly cross-protective (very similar immunogenicity in African and Asian strains). Data indicate that immunogenicity was higher than the recently reported inactivated Zika virus vaccine candidate (Larocca, et. al, 2016, supra.). Inactivated viruses are among the safest vaccines and especially preferred for deliver to populations where safety is especially concerning, such as pregnant women, children and immunocompromised individuals, which makes the herein disclosed inactivated Zika virus particularly suitable. Obtaining a high titer of inactivated virus is a challenge in the field. The herein disclosed process for purifying inactivated Zika virus results in not only a high yield, but also a very pure drug substance.

Example 3: Development of a Purification Process for Yellow Fever Virus Vaccine Produced in Vero Cells

A downstream process was developed for the purification of infectious yellow fever virus particles whereby host cell nucleic acids, non-infectious virus particles and aggregates are removed by the addition of protamine sulphate as described in Examples 1 and 2. The unexpected and novel purification properties of protamine sulphate (PS) were evaluated in purification processes for yellow fever (YF) as follows:

As before the treatment of YF-harvest with PS significantly reduces the amount of aggregates as seen with SEC for two vaccine strains currently in development (FIG. 29).

Further more detailed aspects of the invention:

A1. A process of purification of infectious alphavirus particles, preferably Chikungunya virus particles, comprising the steps of: a) providing a crude harvest (a) comprising virus particles and impurities, wherein the impurities are generated from growing said virus particles on a cell substrate; b) reducing impurities from the crude harvest (a) by precipitation with an agent comprising a protamine salt, preferably a protamine sulphate, even more preferably a recombinant protamine sulphate, to obtain a virus preparation (b); c) contacting the virus preparation (b) with (i) a solid-phase matrix packed in a column comprising a ligand-activated core and an inactive shell comprising pores, wherein the molecular weight cut off of the pores excludes the virus particles from entering the ligand-activated core, and wherein a molecule smaller than the molecular weight cut-off of the pores can enter the ligand-activated core and collecting the virus particles to obtain a virus preparation (d), or (ii) a solid-phase matrix comprising a ligand-activated core and an inactive shell comprising pores, wherein the molecular weight cut off of the pores excludes the virus particles from entering the ligand-activated core, and wherein a molecule smaller than the molecular weight cut-off of the pores can enter the ligand-activated core and separating the solid-phase matrix from the virus particles by filtration to produce a virus preparation (c); and d) further purifying the virus preparation (c) by sucrose density gradient centrifugation to obtain a virus preparation (d) comprising the infectious virus particles, wherein the residual host cell DNA of the virus preparation (d) is less than 100 ng/mL and the residual host cell protein of the final virus preparation (d) is less than 1 .mu.g/mL.

A2. The process of A1, wherein the residual host cell DNA of the virus preparation (d) is less than 10 ng/mL and the residual host cell protein of the final virus preparation (d) is less than 100 ng/mL.

A3. The process of A1 or A2, wherein the crude harvest (a) comprising virus particles and impurities is subjected to one or more pre-purification step(s) prior to step (b).

A4. The process of any one of A1 to A3, wherein the one or more pre-purification step(s) comprises a) filtration using a filter having a pore size equal to or less than 0.2 .mu.m; and/or b) digestion of host cell genomic DNA by enzymatic treatment; and/or c) ultra/diafiltration using a hollow fiber membrane having a pore size equal to or greater than 300 kDa, preferably equal to or greater than 100 kDa.

A5. The process of any one of A1 to A4, wherein the concentration of protamine sulphate is 1 to 2 mg/ml, more preferably 1.2 to 1.8 mg/ml, more preferably 1.4 to 1.6 mg/ml, most preferably 1.6 mg/ml.

A6. The process of any one of A1 to A5, wherein the molecule entering the core of the solid-phase matrix has a molecular weight less than 700 kDa.

A7. The process of any one of A1 to A6, wherein the ligand of the ligand-activated core of the solid-phase matrix is capable of binding the molecule that enters the ligand-activated core via cationic-, anionic-, hydrophobic- or mixed interactions.

A8. The process of any one of A1 to A7, wherein the ligand of the ligand-activated core of the solid-phase matrix is octylamine.

A9. The process of any one of A1 to A8, wherein the solid-phase matrix is used as a slurry and at a final concentration between 0.5% (v/v) and 10% (v/v), preferably 0.6%, 0.7%, 0.9%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, most preferably 1%.

A10. The process of any one of A1 to A9, wherein the solid-phase matrix is incubated with the protamine-treated virus preparation (b) at refrigerated temperatures (2.degree. C. to 8.degree. C.) with a stirring for at least 10 minutes, preferably 15 minutes, 30 minutes or 1 hour, most preferably 15 minutes.

A11. The process of any one of A1 to A10, wherein the enrichment of infectious virus particles in the final virus preparation relative to total virus products in the crude harvest (a) is in the range from at least 50% to 95%, preferably at least 80%.

A12. The process of any one A1 to A11, wherein the filtration of step (c) of preferred aspect 1 is performed using a filter having a pore size equal to or less than 1 .mu.m, preferably 0.2 .mu.m.

A13. The process of any one of A1 to A12, wherein the residual impurity of the final virus preparation is less than 10%.

A14. The process of any one of A1 to A13, wherein the virus is propagated in a cell line selected from the group consisting of an EB66 cell line, a Vero cell line, a Vero-.alpha.His cell line, a HeLa cell line, a HeLa-S3 cell line, a 293 cell line, a PC12 cell line, a CHO cell line, a 3T3 cell line, a PerC6 cell line, a MDSK cell line, a chicken embryonic fibroblast cell line, a duck cell line, and a diploid avian cell line.

A15. The process of A14, wherein said cell line is a Vero cell line.

A16. The process of any one of A1 to A15, wherein the Chikungunya virus is a live virus, an attenuated live virus, a chimeric virus, a modified live virus, or a recombinant live virus.

A17. The process of any one of A1 to A16, wherein the Chikungunya virus is the .DELTA.5nsP3 attenuated mutant or an immunogenic variant thereof.

A18. The process of any one of A1 to A17, wherein said process resulting in final virus preparation (d) is followed by an inactivation step, wherein the virus is inactivated preferably by formaldehyde.

A19. Use of the process according to any one of A1 to A18 for manufacturing a composition for immunization against a Chikungunya virus infection.

A20. The use according to A19, wherein the composition for immunization against a Chikungunya virus infection is a vaccine.

A21. A composition comprising the virus particles obtainable by the process of any one of A1 to A18 for treating and/or preventing a Chikungunya virus infection.

N1. A process of purification of infectious alphavirus particles, preferably Chikungunya virus particles, comprising the steps of: (a) providing a crude harvest (a) comprising virus particles and impurities, wherein the impurities are generated from growing said virus particles on a cell substrate; (b) reducing impurities from the crude harvest (a) by precipitation with an agent comprising a protamine salt, preferably a protamine sulphate, even more preferably a recombinant protamine sulphate, to obtain a virus preparation (b); (c) contacting the virus preparation (b) with (i) a solid-phase matrix packed in a column comprising a ligand-activated core and an inactive shell comprising pores, wherein the molecular weight cut off of the pores excludes the virus particles from entering the ligand-activated core, and wherein a molecule smaller than the molecular weight cut-off of the pores can enter the ligand-activated core and collecting the virus particles to obtain a virus preparation (d), or (ii) a solid-phase matrix comprising a ligand-activated core and an inactive shell comprising pores, wherein the molecular weight cut off of the pores excludes the virus particles from entering the ligand-activated core, and wherein a molecule smaller than the molecular weight cut-off of the pores can enter the ligand-activated core and separating the solid-phase matrix from the virus particles by filtration to produce a virus preparation (c); and (d) further purifying the virus preparation (c) by sucrose density gradient centrifugation to obtain a virus preparation (d) comprising the infectious virus particles, wherein the residual host cell DNA of the virus preparation (d) is less than 100 ng/mL and the residual host cell protein of the final virus preparation (d) is less than 1 .mu.g/mL.

N2. The process of N1, wherein the residual host cell DNA of the virus preparation (d) is less than 10 ng/mL and the residual host cell protein of the final virus preparation (d) is less than 100 ng/mL.

N3. The process of N1 or 2, wherein the crude harvest (a) comprising virus particles and impurities is subjected to one or more pre-purification step(s) prior to step (b).

N4. The process of any one of N1 to 3, wherein the one or more pre-purification step(s) comprises

(a) filtration using a filter having a pore size equal to or less than 0.2 .mu.m; and/or

(b) digestion of host cell genomic DNA by enzymatic treatment; and/or

(c) ultra/diafiltration using a hollow fiber membrane having a pore size equal to or greater than 300 kDa, preferably equal to or greater than 100 kDa.

N5. The process of any one of N1 to 4, wherein the concentration of protamine sulphate is 1 to 2 mg/ml, more preferably 1.2 to 1.8 mg/ml, more preferably 1.4 to 1.6 mg/ml, most preferably 1.6 mg/ml.

N6. The process of any one of N1 to 5, wherein the molecule entering the core of the solid-phase matrix has a molecular weight less than 700 kDa.

N7. The process of any one of N1 to 6, wherein the ligand of the ligand-activated core of the solid-phase matrix is capable of binding the molecule that enters the ligand-activated core via cationic-, anionic-, hydrophobic- or mixed interactions.

N8. The process of any one of N1 to 7, wherein the ligand of the ligand-activated core of the solid-phase matrix is octylamine.

N9. The process of any one of N1 to 8, wherein the solid-phase matrix is used as a slurry and at a final concentration between 0.5% (v/v) and 10% (v/v), preferably 0.6%, 0.7%, 0.9%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, most preferably 1%.

N10. The process of any one of N1 to 9, wherein the solid-phase matrix is incubated with the protamine-treated virus preparation (b) at refrigerated temperatures (2.degree. C. to 8.degree. C.) with a stirring for at least 10 minutes, preferably 15 minutes, 30 minutes or 1 hour, most preferably 15 minutes.

N11. The process of any one of N1 to 10, wherein the enrichment of infectious virus particles in the final virus preparation relative to total virus products in the crude harvest (a) is in the range from at least 50% to 95%, preferably at least 80%.

N12. The process of any one of N1 to 11, wherein the filtration of step (c) of N1 is performed using a filter having a pore size equal to or less than 1 .mu.m, preferably 0.2 .mu.m.

N13. The process of any one of N1 to 12, wherein the residual impurity of the final virus preparation is less than 10%.

N14. The process of any one of N1 to 13, wherein the virus is propagated in a cell line selected from the group consisting of an EB66 cell line, a Vero cell line, a Vero-.alpha.His cell line, a HeLa cell line, a HeLa-S3 cell line, a 293 cell line, a PC12 cell line, a CHO cell line, a 3T3 cell line, a PerC6 cell line, a MDSK cell line, a chicken embryonic fibroblast cell line, a duck cell line, and a diploid avian cell line.

N15. The process of N14, wherein said cell line is a Vero cell line.

N16. The process of any one of N1 to 15, wherein the Chikungunya virus is a live virus, an attenuated live virus, a chimeric virus, a modified live virus, or a recombinant live virus.

N17. The process of any one of N1 to 16, wherein the Chikungunya virus is the .DELTA.5nsP3 attenuated mutant or an immunogenic variant thereof.

N18. The process of any one of N1 to 17, wherein said process resulting in final virus preparation (d) is followed by an inactivation step, wherein the virus is inactivated preferably by formaldehyde.

N19. Use of the process according to any one of N1 to 18 for manufacturing a composition for immunization against a Chikungunya virus infection.

N20. The use according to N19, wherein the composition for immunization against a Chikungunya virus infection is a vaccine.

N21. A composition comprising the virus particles obtainable by the process of any one of N1 to 18 for treating and/or preventing a Chikungunya virus infection.

P1. A Zika virus vaccine comprising an optimally inactivated Zika virus particle, wherein the Zika virus particle is able to seroconvert a subject that is administered the Zika virus vaccine with at least a 70% probability.

P2. The Zika virus vaccine of P1, wherein the Zika virus particle is able to seroconvert the subject that is administered the Zika virus vaccine with at least a 80%, 85%, 90%, or 95% probability, preferably a 80% probability.

P3. The vaccine of P1 or 2, wherein the Zika virus particle has a RNA genome corresponding to the DNA sequence provided by any one of the nucleic acid sequences of SEQ ID NOs: 2-13, or a variant nucleic acid sequence that is at least 88% identical to any one of SEQ ID NOs: 2-13 and able to pack a virulent Zika virus.

P4. The vaccine of any one of P1-3, wherein the Zika virus particle has an E protein selected from the amino acid sequences provided by any one of SEQ ID NOs: 14-69, or a variant amino acid sequence that is at least 95% identical to any one of SEQ ID NOs: 14-69 and able to pack a virulent Zika virus.

P5. The vaccine of any one of P1-4, wherein the Zika virus is inactivated by chemical inactivation, thermal inactivation, pH inactivation, or UV inactivation.

P6. The vaccine of P5, wherein the chemical inactivation comprises contacting the Zika virus with a chemical inactivation agent for longer than is required to completely inactivate the Zika virus as measured by plaque assay.

P7. The vaccine of P6, wherein the chemical inactivation comprises contacting the Zika virus with formaldehyde.

P8. The vaccine of P7, wherein the formaldehyde inactivation comprises contacting the Zika virus with formaldehyde for between 2-10 days.

P9. The vaccine of any one of P5-8, wherein the chemical activation is performed at about +4.degree. C. or about +22.degree. C.

P10. The vaccine of any one of P1-9, further comprising an adjuvant.

P11. The vaccine of P10, wherein the adjuvant is an aluminum salt adjuvant.

P12. The vaccine of P11, wherein the aluminum salt adjuvant is aluminium hydroxide or aluminium phosphate salt.

P13. The vaccine of any one of P10-12, wherein the vaccine comprises or further comprises an adjuvant comprising a peptide and a deoxyinosine-containing immunostimulatory oligodeoxynucleic acid molecule (I-ODN).

P14. The vaccine of P13, wherein the peptide comprises the sequence KLKL5KLK (SEQ ID NO: 71) and the I-ODN comprises oligo-d(IC)13 (SEQ ID NO: 70).

P15. The vaccine of any one of P1-14, further comprising one or more pharmaceutically acceptable excipient.

Q1. A process of purification of infectious virus particles, comprising the steps of:

(a) providing a crude harvest (a) comprising virus particles and impurities, wherein the impurities are generated from growing said virus particles on a cell substrate;

(b) reducing impurities from the crude harvest (a) by precipitation with an agent comprising protamine, preferably a protamine salt, more preferably a protamine sulphate, even more preferably a recombinant protamine sulphate, to obtain a virus preparation (b);

(c) further purifying the virus preparation (b) by an optimized sucrose density gradient centrifugation, wherein the optimized sucrose gradient is provided such that the protamine can be completely or almost completely separated from the virus fraction; and wherein the protamine concentration is reduced by this step to the extent that the protamine concentration in the final drug substance is below 1 .mu.g/ml, preferably below 0.5 .mu.g/mL, more preferably below 0.1 .mu.g/mL, most preferably below 0.05 .mu.g/mL.

Q2. The process of Q2, wherein the virus particles are selected from the group consisting of flaviviruses, e.g. yellow fever virus or Zika virus and alphaviruses, e.g. Chikungunya.

Q3. The process of Q1 or Q2, additionally comprising the step of:

(d) a solid-phase matrix packed in a column comprising a ligand-activated core and an inactive shell comprising pores, wherein the molecular weight cut off of the pores excludes the virus particles from entering the ligand-activated core, and wherein a molecule smaller than the molecular weight cutoff of the pores can enter the ligand-activated core and collecting the virus particles.

Q4. The process of any of Q1 to 3, wherein the residual host cell DNA of the virus preparation (c) is less than 10 ng/mL and the residual host cell protein of the final virus preparation (c) is less than 100 ng/mL.

Q5. The process of any of Q1 to 4, wherein the crude harvest (a) comprising virus particles and impurities is subjected to one or more pre-purification step(s) prior to step (b).

Q6. The process of Q5, wherein the one or more pre-purification step(s) comprises

(a) filtration using a filter having a pore size equal to or less than 0.2 .mu.m; and/or

(b) digestion of host cell genomic DNA by enzymatic treatment; and/or

(c) ultra/diafiltration using a hollow fiber membrane having a pore size equal to or greater than 300 kDa, preferably equal to or greater than 100 kDa.

Q7. The process of any one of Q1 to 6, wherein the concentration of protamine sulphate is 0.5 to 3 mg/ml, more preferably 1 to 2 mg/ml, more preferably 1.2 to 1.8 mg/ml, more preferably 1.4 to 1.6 mg/ml, most preferably 1.6 mg/ml or 2 mg/ml.

Q8. The process of any one of Q1 to 7, wherein the enrichment of infectious virus particles in the virus preparation (c) or any final virus preparation relative to total virus products in the crude harvest (a) is in the range from at least 50% to 95%, preferably at least 80%.

Q9. The process of any one of Q5 to 8, wherein the one or more pre-purification step(s) prior to step (b) of any of Q5 to 8 is performed using a filter having a pore size equal to or less than 1 .mu.m, preferably 0.2 .mu.m.

Q10. The process of any one of Q1 to 9, wherein the residual impurity of the virus preparation

(c) is less than 10%.

Q11. The process of any one of Q1 to 10, wherein the virus is propagated in a cell line selected from the group consisting of an EB66 cell line, a Vero cell line, a Vero-.alpha.His cell line, a HeLa cell line, a HeLa-S3 cell line, a 293 cell line, a PC12 cell line, a CHO cell line, a 3T3 cell line, a PerC6 cell line, a MDSK cell line, a chicken embryonic fibroblast cell line, a duck cell line, and a diploid avian cell line.

Q12. The process of Q11, wherein said cell line is a Vero cell line.

Q13. The process of any one of Q1 to 12, wherein the infectious virus particles is an infectious Zika virus particle that is a live virus, an attenuated live virus, a chimeric virus, a modified live virus, or a recombinant live virus.

Q14. The process of any one of Q1 to 13, wherein the Zika virus is a Zika virus strain of the Asian lineage or an immunogenic variant thereof.

Q15. The process of any one of Q1 to 14, wherein said process resulting in final virus preparation (c) or (d) is followed by an inactivation step, wherein the virus is inactivated preferably by formaldehyde.

Q16. Use of the process according to any one of Q1 to 15 for manufacturing a composition for immunization against a virus infection.

Q17. The use according to Q16, wherein the composition for immunization against a virus infection is an infection caused by a group of viruses consisting of yellow fever virus, Chikungunya virus and Zika virus.

Q18. A composition comprising the virus particles obtainable or obtained by the process of any one of Q1 to 17 for treating and/or preventing an infection, such as e.g. a Zika virus infection.

Q19. A Zika virus vaccine comprising an inactivated Zika virus particle grown on vero cells, wherein the Zika virus particle is able to seroconvert a subject that is administered the Zika virus vaccine with at least a 70% probability and comprises minor amounts of protamine sulphate, preferably below the detection limit.

Q20. The Zika virus vaccine of Q19, wherein the Zika virus particle is able to seroconvert the subject that is administered the Zika virus vaccine with at least a 80%, 85%, 90%, or 95% probability, preferably a 80% probability.

Q21. The vaccine of Q19 or 20, wherein the Zika virus particle has a RNA genome corresponding to the DNA sequence provided by any one of the nucleic acid sequences of SEQ ID NOs: 2-13, or a variant nucleic acid sequence that is at least 88% identical to any one of SEQ ID NOs: 2-13 and able to pack a virulent Zika virus.

Q22. The vaccine of any one of Q19, 20 and 21, wherein the Zika virus particle has an E protein selected from the amino acid sequences provided by any one of SEQ ID NOs: 14-69, or a variant amino acid sequence that is at least 95% identical to any one of SEQ ID NOs: 14-69 and able to pack a virulent Zika virus.

Q23. The vaccine of any one of Q19, 20 to 22, wherein the Zika virus obtained by culturing on Vero cells is purified by protamine sulfate precipitation and sucrose gradient centrifugation.

Q24. The vaccine of Q23, wherein the sucrose gradient centrifugation is an optimized sucrose gradient centrifugation.

Q25. The vaccine of Q24, wherein the optimized sucrose gradient centrifugation comprises a virus comprising fraction in a 10% (w/w) sucrose solution and three layers of sucrose with different densities, i.e. a first sucrose solution with 15% (w/w) sucrose solution, a second sucrose solution with 35% (w/w) sucrose solution, and a third sucrose solution with a 50% (w/w) sucrose solution.

Q26. The vaccine of any one of Q19, 20 to 25, wherein the Zika virus is inactivated by chemical inactivation, thermal inactivation, pH inactivation, or UV inactivation.

Q27. The vaccine of Q26, wherein the chemical inactivation comprises contacting the Zika virus with a chemical inactivation agent for longer than is required to completely inactivate the Zika virus as measured by plaque assay.

Q28. The vaccine of Q27, wherein the chemical inactivation comprises contacting the Zika virus with formaldehyde.

Q29. The vaccine of Q28, wherein the formaldehyde inactivation comprises contacting the Zika virus with formaldehyde for between 2-10 days.

Q30. The vaccine of any one of Q27-29, wherein the chemical activation is performed at about +4.degree. C. or about +22.degree. C.

Q31. The vaccine of any one of Q19 to 30, further comprising an adjuvant.

Q32. The vaccine of Q31, wherein the adjuvant is an aluminum salt adjuvant.

Q33. The vaccine of Q32, wherein the aluminum salt adjuvant is aluminium hydroxide or aluminium phosphate salt.

Q34. The vaccine of Q32, wherein the aluminum salt adjuvant is aluminium hydroxide with less than 1.25 ppb Cu based on the final pharmaceutical composition comprising the Zika virus, preferably the inactivated Zika virus.

Q35. The vaccine of any one of Q19 to 34, further comprising one or more pharmaceutically acceptable excipient.

R1. Use of protamine, preferably a protamine salt, to separate infectious and non-infectious virus particles, host cell proteins and/or undefined low molecular weight materials.

R2. A process of purification of infectious virus particles, comprising the steps of:

(a) providing a crude harvest (a) comprising virus particles and impurities, wherein the impurities are generated from growing said virus particles on a cell substrate;

(b) reducing impurities from the crude harvest (a) by precipitation with an agent comprising protamine, preferably a protamine salt, more preferably a protamine sulphate, even more preferably a recombinant protamine sulphate, to obtain a virus preparation (b), wherein the enrichment of infectious virus particles in the virus preparation (b) relative to total virus products in the crude harvest (a) is in the range from at least 50% to 95%, preferably at least 80%.

R3. The use of R1 or the process of R2, wherein the virus particles are selected from the group consisting of flaviviruses, e.g. yellow fever virus or Zika virus and alphaviruses, e.g.

Chikungunya.

R4. A process of purification of infectious virus particles, comprising the steps of:

(a) providing a crude harvest (a) comprising virus particles and impurities, wherein the impurities are generated from growing said virus particles on a cell substrate;

(b) reducing impurities from the crude harvest (a) by precipitation with an agent comprising protamine, preferably a protamine salt, more preferably a protamine sulphate, even more preferably a recombinant protamine sulphate, to obtain a virus preparation (b);

(c) further purifying the virus preparation (b) by one or more size exclusion methods such as (i) a sucrose density gradient centrifugation, (ii) a solid-phase matrix packed in a column comprising a ligand-activated core and an inactive shell comprising pores, wherein the molecular weight cut off of the pores excludes the virus particles from entering the ligand-activated core, and wherein a molecule smaller than the molecular weight cutoff of the pores can enter the ligand-activated core and collecting the virus particles, and/or (iii) size exclusion chromatography to obtain a virus preparation (c) comprising the infectious virus particles, wherein the residual host cell DNA of the virus preparation (c) is less than 100 ng/mL and the residual host cell protein and the residual aggregates of infectious virus particles of the final virus preparation (c) is less than 1 .mu.g/mL.

R5. The process of R4, wherein the residual host cell DNA of the virus preparation (c) is less than 10 ng/mL and the residual host cell protein of the final virus preparation (c) is less than 100 ng/mL.

R6. The process of any of R2 to 5, wherein the crude harvest (a) comprising virus particles and impurities is subjected to one or more pre-purification step(s) prior to step (b).

R7. The process of R6, wherein the one or more pre-purification step(s) comprises

(a) filtration using a filter having a pore size equal to or less than 0.2 .mu.m; and/or

(b) digestion of host cell genomic DNA by enzymatic treatment; and/or

(c) ultra/diafiltration using a hollow fiber membrane having a pore size equal to or greater than 300 kDa, preferably equal to or greater than 100 kDa.

R8. The process of any one of R2 to 7, wherein the concentration of protamine sulphate is 0.5 to 3 mg/ml, more preferably 1 to 2 mg/ml, more preferably 1.2 to 1.8 mg/ml, more preferably 1.4 to 1.6 mg/ml, most preferably 1.6 mg/ml.

R9. The process of any one of R2 to 8, wherein the enrichment of infectious virus particles in the virus preparation (c) or any final virus preparation relative to total virus products in the crude harvest (a) is in the range from at least 50% to 95%, preferably at least 80%.

R10. The process of any one of R6 to 9, wherein the one or more pre-purification step(s) prior to step (b) of any of R6 to 9 is performed using a filter having a pore size equal to or less than 1 .mu.m, preferably 0.2 .mu.m.

R11. The process of any one of R2 to 10, wherein the residual impurity of the virus preparation (c) is less than 10%.

R12. The process of any one of R2 to 11, wherein the virus is propagated in a cell line selected from the group consisting of an EB66 cell line, a Vero cell line, a Vero-.alpha.His cell line, a HeLa cell line, a HeLa-S3 cell line, a 293 cell line, a PC12 cell line, a CHO cell line, a 3T3 cell line, a PerC6 cell line, a MDSK cell line, a chicken embryonic fibroblast cell line, a duck cell line, and a diploid avian cell line.

R13. The process of R12, wherein said cell line is a Vero cell line.

R14. The process of any one of R2 to 13, wherein the Zika virus is a live virus, an attenuated live virus, a chimeric virus, a modified live virus, or a recombinant live virus.

R15. The process of any one of R2 to 14, wherein the Zika virus is a Zika virus strain of the Asian lineage or an immunogenic variant thereof.

R16. The process of any one of R2 to 15, wherein said process resulting in final virus preparation (c) is followed by an inactivation step, wherein the virus is inactivated preferably by formaldehyde.

R17. Use of the process according to any one of R1 to 16 for manufacturing a composition for immunization against a virus infection.

R18. The use according to R17, wherein the composition for immunization against a virus infection is an infection caused by a group of viruses consisting of yellow fever virus, Chikungunya virus and Zika virus.

R19. A composition comprising the virus particles obtainable or obtained by the process of any one of R2 to 16 for treating and/or preventing an infection.

SEQUENCE LISTINGS

1

123132PRTArtificial Sequencesynthetic peptide 1Pro Arg Arg Arg Arg Ser Ser Ser Arg Pro Val Arg Arg Arg Arg Arg1 5 10 15Pro Arg Val Ser Arg Arg Arg Arg Arg Arg Gly Gly Arg Arg Arg Arg 20 25 30210676DNAZika virus 2gttgttactg ttgctgactc agactgcgac agttcgagtt tgaagcgaaa gctagcaaca 60gtatcaacag gttttatttg gatttggaaa cgagagtttc tggtcatgaa aaacccaaaa 120aagaaatccg gaggattccg gattgtcaat atgctaaaac gcggagtagc ccgtgtgagc 180ccctttgggg gcttgaagag gctgccagcc ggacttctgc tgggtcatgg gcccatcagg 240atggtcttgg caattctagc ctttttgaga ttcacggcaa tcaagccatc actgggtctc 300atcaatagat ggggttcagt ggggaaaaaa gaggctatgg aaataataaa gaagttcaag 360aaagatctgg ctgccatgct gagaataatc aatgctagga aggagaagaa gagacggggc 420gcagatacta gtgtcggaat tgttggcctc ctgctgacca cagctatggc agcggaggtc 480actagacgtg ggagtgcata ctatatgtac ttggacagaa acgatgctgg ggaggccata 540tcttttccaa ccacattggg gatgaataag tgttatatac agatcatgga tcttggacac 600atgtgtgatg ccaccatgag ctatgaatgc cctatgctgg atgagggggt ggaaccagat 660gacgtcgatt gttggtgcaa cacgacgtca acttgggttg tgtacggaac ctgccatcac 720aaaaaaggtg aagcacggag atctagaaga gctgtgacgc tcccctccca ttccactagg 780aagctgcaaa cgcggtcgca aacctggttg gaatcaagag aatacacaaa gcacttgatt 840agagtcgaaa attggatatt caggaaccct ggcttcgcgt tagcagcagc tgccatcgct 900tggcttttgg gaagctcaac gagccaaaaa gtcatatact tggtcatgat actgctgatt 960gccccggcat acagcatcag gtgcatagga gtcagcaata gggactttgt ggaaggtatg 1020tcaggtggga cttgggttga tattgtcttg gaacatggag gttgtgtcac cgtaatggca 1080caggacaaac cgactgtcga catagagctg gttacaacaa cagtcagcaa catggcggag 1140gtaagatcct actgctatga ggcatcaata tcagacatgg cttcggacag ccgctgccca 1200acacaaggtg aagcctacct tgacaagcaa tcagacactc aatatgtctg caaaagaacg 1260ttagtggaca gaggctgggg aaatggatgt ggactttttg gcaaagggag tctggtgaca 1320tgcgctaagt ttgcatgctc caagaaaatg accgggaaga gcatccagcc agagaatctg 1380gagtaccgga taatgctgtc agttcatggc tcccagcaca gtgggatgat cgttaatgac 1440acaggacatg aaactgatga gaatagagcg aaggttgaga taacgcccaa ttcaccaaga 1500gccgaagcca ccctgggggg ttttggaagc ctaggacttg attgtgaacc gaggacaggc 1560cttgactttt cagatttgta ttacttgact atgaataaca agcactggtt ggttcacaag 1620gagtggttcc acgacattcc attaccttgg cacgctgggg cagacaccgg aactccacac 1680tggaacaaca aagaagcact ggtagagttc aaggacgcac atgccaaaag gcaaactgtc 1740gtggttctag ggagtcaaga aggagcagtt cacacggccc ttgctggagc tctggaggct 1800gagatggatg gtgcaaaggg aaggctgtcc tctggccact tgaaatgtcg cctgaaaatg 1860gataaactta gattgaaggg cgtgtcatac tccttgtgta ccgcagcgtt cacattcacc 1920aagatcccgg ctgaaacact gcacgggaca gtcacagtgg aggtacagta cgcagggaca 1980gatggacctt gcaaggttcc agctcagatg gcggtggaca tgcaaactct gaccccagtt 2040gggaggttga taaccgctaa ccccgtaatc actgaaagca ctgagaactc taagatgatg 2100ctggaacttg atccaccatt tggggactct tacattgtca taggagtcgg ggagaagaag 2160atcacccacc actggcacag gagtggcagc accattggaa aagcatttga agccactgtg 2220agaggtgcca agagaatggc agtcttggga gacacagcct gggactttgg atcagttgga 2280ggcgctctca actcattggg caagggcatc catcaaattt ttggagcagc tttcaaatca 2340ttgtttggag gaatgtcctg gttctcacaa attctcattg gaacgttgct gatgtggttg 2400ggtctgaaca caaagaatgg atctatttcc cttatgtgct tggccttagg gggagtgttg 2460atcttcttat ccacagccgt ctctgctgat gtggggtgct cggtggactt ctcaaagaag 2520gagacgagat gcggtacagg ggtgttcgtc tataacgacg ttgaagcctg gagggacagg 2580tacaagtacc atcctgactc cccccgtaga ttggcagcag cagtcaagca agcctgggaa 2640gatggtatct gcgggatctc ctctgtttca agaatggaaa acatcatgtg gagatcagta 2700gaaggggagc tcaacgcaat cctggaagag aatggagttc aactgacggt cgttgtggga 2760tctgtaaaaa accccatgtg gagaggtcca cagagattgc ccgtgcctgt gaacgagctg 2820ccccacggct ggaaggcttg ggggaaatcg cacttcgtca gagcagcaaa gacaaataac 2880agctttgtcg tggatggtga cacactgaag gaatgcccac tcaaacatag agcatggaac 2940agctttcttg tggaggatca tgggttcggg gtatttcaca ctagtgtctg gctcaaggtt 3000agagaagatt attcattaga gtgtgatcca gccgttattg gaacagctgt taagggaaag 3060gaggctgtac acagtgatct aggctactgg attgagagtg agaagaatga cacatggagg 3120ctgaagaggg cccatctgat cgagatgaaa acatgtgaat ggccaaagtc ccacacattg 3180tggacagatg gaatagaaga gagtgatctg atcataccca agtctttagc tgggccactc 3240agccatcaca ataccagaga gggctacagg acccaaatga aagggccatg gcacagtgaa 3300gagcttgaaa ttcggtttga ggaatgccca ggcactaagg tccacgtgga ggaaacatgt 3360ggaacaagag gaccatctct gagatcaacc actgcaagcg gaagggtgat cgaggaatgg 3420tgctgcaggg agtgcacaat gcccccactg tcgttccggg ctaaagatgg ctgttggtat 3480ggaatggaga taaggcccag gaaagaacca gaaagcaact tagtaaggtc aatggtgact 3540gcaggatcaa ctgatcacat ggatcacttc tcccttggag tgcttgtgat tctgctcatg 3600gtgcaggaag ggctgaagaa gagaatgacc acaaagatca tcataagcac atcaatggca 3660gtgctggtag ctatgatcct gggaggattt tcaatgagtg acctggctaa gcttgcaatt 3720ttgatgggtg ccaccttcgc ggaaatgaac actggaggag atgtagctca tctggcgctg 3780atagcggcat tcaaagtcag accagcgttg ctggtatctt tcatcttcag agctaattgg 3840acaccccgtg aaagcatgct gctggccttg gcctcgtgtc ttttgcaaac tgcgatctcc 3900gccttggaag gcgacctgat ggttctcatc aatggttttg ctttggcctg gttggcaata 3960cgagcgatgg ttgttccacg cactgataac atcaccttgg caatcctggc tgctctgaca 4020ccactggccc ggggcacact gcttgtggcg tggagagcag gccttgctac ttgcgggggg 4080tttatgctcc tctctctgaa gggaaaaggc agtgtgaaga agaacttacc atttgtcatg 4140gccctgggac taaccgctgt gaggctggtc gaccccatca acgtggtggg gctgctgttg 4200ctcacaagga gtgggaagcg gagctggccc cctagcgaag tactcacagc tgttggcctg 4260atatgcgcat tggctggagg gttcgccaag gcagatatag agatggctgg gcccatggcc 4320gcggtcggtc tgctaattgt cagttacgtg gtctcaggaa agagtgtgga catgtacatt 4380gaaagagcag gtgacatcac atgggaaaaa gatgcggaag tcactggaaa cagtccccgg 4440ctcgatgtgg cgctagatga gagtggtgat ttctccctgg tggaggatga cggtcccccc 4500atgagagaga tcatactcaa ggtggtcctg atgaccatct gtggcatgaa cccaatagcc 4560ataccctttg cagctggagc gtggtacgta tacgtgaaga ctggaaaaag gagtggtgct 4620ctatgggatg tgcctgctcc caaggaagta aaaaaggggg agaccacaga tggagtgtac 4680agagtaatga ctcgtagact gctaggttca acacaagttg gagtgggagt tatgcaagag 4740ggggtctttc acactatgtg gcacgtcaca aaaggatccg cgctgagaag cggtgaaggg 4800agacttgatc catactgggg agatgtcaag caggatctgg tgtcatactg tggtccatgg 4860aagctagatg ccgcctggga cgggcacagc gaggtgcagc tcttggccgt gccccccgga 4920gagagagcga ggaacatcca gactctgccc ggaatattta agacaaagga tggggacatt 4980ggagcggttg cgctggatta cccagcagga acttcaggat ctccaatcct agacaagtgt 5040gggagagtga taggacttta tggcaatggg gtcgtgatca aaaatgggag ttatgttagt 5100gccatcaccc aagggaggag ggaggaagag actcctgttg agtgcttcga gccttcgatg 5160ctgaagaaga agcagctaac tgtcttagac ttgcatcctg gagctgggaa aaccaggaga 5220gttcttcctg aaatagtccg tgaagccata aaaacaagac tccgtactgt gatcttagct 5280ccaaccaggg ttgtcgctgc tgaaatggag gaagccctta gagggcttcc agtgcgttat 5340atgacaacag cagtcaatgt cacccactct ggaacagaaa tcgtcgactt aatgtgccat 5400gccaccttca cttcacgtct actacagcca atcagagtcc ccaactataa tctgtatatt 5460atggatgagg cccacttcac agatccctca agtatagcag caagaggata catttcaaca 5520agggttgaga tgggcgaggc ggctgccatc ttcatgaccg ccacgccacc aggaacccgt 5580gacgcatttc cggactccaa ctcaccaatt atggacaccg aagtggaagt cccagagaga 5640gcctggagct caggctttga ttgggtgacg gattattctg gaaaaacagt ttggtttgtt 5700ccaagcgtga ggaacggcaa tgagatcgca gcttgtctga caaaggctgg aaaacgggtc 5760atacagctca gcagaaagac ttttgagaca gagttccaga aaacaaaaca tcaagagtgg 5820gactttgtcg tgacaactga catttcagag atgggcgcca actttaaagc tgaccgtgtc 5880atagattcca ggagatgcct aaagccggtc atacttgatg gcgagagagt cattctggct 5940ggacccatgc ctgtcacaca tgccagcgct gcccagagga gggggcgcat aggcaggaat 6000cccaacaaac ctggagatga gtatctgtat ggaggtgggt gcgcagagac tgacgaagac 6060catgcacact ggcttgaagc aagaatgctc cttgacaata tttacctcca agatggcctc 6120atagcctcgc tctatcgacc tgaggccgac aaagtagcag ccattgaggg agagttcaag 6180cttaggacgg agcaaaggaa gacctttgtg gaactcatga aaagaggaga tcttcctgtt 6240tggctggcct atcaggttgc atctgccgga ataacctaca cagatagaag atggtgcttt 6300gatggcacga ccaacaacac cataatggaa gacagtgtgc cggcagaggt gtggaccaga 6360cacggagaga aaagagtgct caaaccgagg tggatggacg ccagagtttg ttcagatcat 6420gcggccctga agtcattcaa ggagtttgcc gctgggaaaa gaggagcggc ttttggagtg 6480atggaagccc tgggaacact gccaggacac atgacagaga gattccagga agccattgac 6540aacctcgctg tgctcatgcg ggcagagact ggaagcaggc cttacaaagc cgcggcggcc 6600caattgccgg agaccctaga gaccattatg cttttggggt tgctgggaac agtctcgctg 6660ggaatctttt tcgtcttgat gaggaacaag ggcataggga agatgggctt tggaatggtg 6720actcttgggg ccagcgcatg gctcatgtgg ctctcggaaa ttgagccagc cagaattgca 6780tgtgtcctca ttgttgtgtt cctattgctg gtggtgctca tacctgagcc agaaaagcaa 6840agatctcccc aggacaacca aatggcaatc atcatcatgg tagcagtagg tcttctgggc 6900ttgattaccg ccaatgaact cggatggttg gagagaacaa agagtgacct aagccatcta 6960atgggaagga gagaggaggg ggcaaccatg ggattctcaa tggacattga cctgcggcca 7020gcctcagctt gggccatcta tgctgccttg acaactttca ttaccccagc cgtccaacat 7080gcagtgacca cttcatacaa caactactcc ttaatggcga tggccacgca agctggagtg 7140ttgtttggta tgggcaaagg gatgccattc tacgcatggg actttggagt cccgctgcta 7200atgataggtt gctactcaca attaacgccc ctgaccctaa tagtggccat cattttgctc 7260gtggcgcact acatgtactt gatcccaggg ctgcaggcag cagctgcgcg tgctgcccag 7320aagagaacgg cagctggcat catgaagaac cctgttgtgg atggaatagt ggtgactgac 7380attgacacaa tgacaattga cccccaagtg gagaaaaaga tgggacaggt gctactcatg 7440gcagtagccg tctccagcgc catactgtcg cggaccgcct gggggtgggg ggaggctggg 7500gccctgatca cagccgcaac ttccactttg tgggaaggct ctccgaacaa gtactggaac 7560tcctctacag ccacttcact gtgtaacatt tttaggggaa gttacttggc tggagcttct 7620ctaatctaca cagtaacaag aaacgctggc ttggtcaaga gacgtggggg tggaacagga 7680gagaccctgg gagagaaatg gaaggcccgc ttgaaccaga tgtcggccct ggagttctac 7740tcctacaaaa agtcaggcat caccgaggtg tgcagagaag aggcccgccg cgccctcaag 7800gacggtgtgg caacgggagg ccatgctgtg tcccgaggaa gtgcaaagct gagatggttg 7860gtggagcggg gatacctgca gccctatgga aaggtcattg atcttggatg tggcagaggg 7920ggctggagtt actacgccgc caccatccgc aaagttcaag aagtgaaagg atacacaaaa 7980ggaggccctg gtcatgaaga acccgtgttg gtgcaaagct atgggtggaa catagtccgt 8040cttaagagtg gggtggacgt ctttcatatg gcggctgagc cgtgtgacac gttgctgtgt 8100gacataggtg agtcatcatc tagtcctgaa gtggaagaag cacggacgct cagagtcctc 8160tccatggtgg gggattggct tgaaaaaaga ccaggagcct tttgtataaa agtgttgtgc 8220ccatacacca gcactatgat ggaaaccctg gagcgactgc agcgtaggta tgggggagga 8280ctggtcagag tgccactctc ccgcaactct acacatgaga tgtactgggt ctctggagcg 8340aaaagcaaca ccataaaaag tgtgtccacc acgagccagc tcctcttggg gcgcatggac 8400gggcctagga ggccagtgaa atatgaggag gatgtgaatc tcggctctgg cacgcgggct 8460gtggtaagct gcgctgaagc tcccaacatg aagatcattg gtaaccgcat tgaaaggatc 8520cgcagtgagc acgcggaaac gtggttcttt gacgagaacc acccatatag gacatgggct 8580taccatggaa gctatgaggc ccccacacaa gggtcagcgt cctctctaat aaacggggtt 8640gtcaggctcc tgtcaaaacc ctgggatgtg gtgactggag tcacaggaat agccatgacc 8700gacaccacac cgtatggtca gcaaagagtt ttcaaggaaa aagtggacac tagggtgcca 8760gacccccaag aaggtactcg tcaggttatg agcatggtct cttcctggtt gtggaaagag 8820ctaggcaaac acaaacggcc acgagtctgt accaaagaag agttcatcaa caaggttcgt 8880agcaatgcag cattaggggc aatatttgaa gaggaaaaag agtggaagac tgcagtggaa 8940gctgtgaacg atccaaggtt ctgggctcta gtggacaagg aaagagagca ccacctgaga 9000ggagagtgcc agagttgtgt gtacaacatg atgggaaaaa gagaaaagaa acaaggggaa 9060tttggaaagg ccaagggcag ccgcgccatc tggtatatgt ggctaggggc tagatttcta 9120gagttcgaag cccttggatt cttgaacgag gatcactgga tggggagaga gaactcagga 9180ggtggtgttg aagggctggg attacaaaga ctcggatatg tcctagaaga gatgagtcgc 9240ataccaggag gaaggatgta tgcagatgac actgctggct gggacacccg catcagcagg 9300tttgatctgg agaatgaagc tctaatcacc aaccaaatgg agaaagggca cagggccttg 9360gcattggcca taatcaagta cacataccaa aacaaagtgg taaaggtcct tagaccagct 9420gaaaaaggga aaacagttat ggacattatt tcgagacaag accaaagggg gagcggacaa 9480gttgtcactt acgctcttaa cacatttacc aacctagtgg tgcaactcat tcggaatatg 9540gaggctgagg aagtcctaga gatgcaagac ttgtggctgc tgcggaggtc agagaaagtg 9600accaactggt tgcagagcaa cggatgggat aggctcaaac gaatggcagt cagtggagat 9660gattgcgttg tgaagccaat tgatgatagg tttgcacatg ccctcaggtt cttgaatgat 9720atgggaaaag ttaggaagga cacacaagag tggaaaccct caactggatg ggacaactgg 9780gaagaagttc cgttttgctc ccaccacttc aacaagctcc atctcaagga cgggaggtcc 9840attgtggttc cctgccgcca ccaagatgaa ctgattggcc gggcccgcgt ctctccaggg 9900gcgggatgga gcatccggga gactgcttgc ctagcaaaat catatgcgca aatgtggcag 9960ctcctttatt tccacagaag ggacctccga ctgatggcca atgccatttg ttcatctgtg 10020ccagttgact gggttccaac tgggagaact acctggtcaa tccatggaaa gggagaatgg 10080atgaccactg aagacatgct tgtggtgtgg aacagagtgt ggattgagga gaacgaccac 10140atggaagaca agaccccagt tacgaaatgg acagacattc cctatttggg aaaaagggaa 10200gacttgtggt gtggatctct catagggcac agaccgcgca ccacctgggc tgagaacatt 10260aaaaacacag tcaacatggt gcgcaggatc ataggtgatg aagaaaagta catggactac 10320ctatccaccc aagttcgcta cttgggtgaa gaagggtcta cacctggagt gctgtaagca 10380ccaatcttaa tgttgtcagg cctgctagtc agccacagct tggggaaagc tgtgcagcct 10440gtgacccccc caggagaagc tgggaaacca agcctatagt caggccgaga acgccatggc 10500acggaagaag ccatgctgcc tgtgagcccc tcagaggaca ctgagtcaaa aaaccccacg 10560cgcttggagg cgcaggatgg gaaaagaagg tggcgacctt ccccaccctt caatctgggg 10620cctgaactgg agatcagctg tggatctcca gaagagggac tagtggttag aggaga 10676310793DNAZika virus 3ccaatctgtg aatcagactg cgacagttcg agtttgaagc gaaagctagc aacagtatca 60acaggtttta ttttggattt ggaaacgaga gtttctggtc atgaaaaacc caaaaaagaa 120atccggagga ttccggattg tcaatatgct aaaacgcgga gtagcccgtg tgagcccctt 180tgggggcttg aagaggctgc cagccggact tctgctgggt catgggccca tcaggatggt 240cttggcgatt ctagcctttt tgagattcac ggcaatcaag ccatcactgg gtctcatcaa 300tagatggggt tcagtgggga aaaaagaggc tatggaaata ataaagaagt tcaagaaaga 360tctggctgcc atgctgagaa taatcaatgc caggaaggag aagaagagac gaggcgcaga 420tactagtgtc ggaatcgttg gcctcctgct gaccacagct atggcagcgg aggtcactag 480acgtgggagt gcatactata tgtacttgga cagaaacgat gctggggagg ccatatcttt 540tccaaccaca ttggggatga ataagtgtta tatacagatc atggatcttg gacacatgtg 600tgatgccacc atgagctatg aatgccctat gctggatgag ggggtggaac cagatgacgt 660cgattgttgg tgcaacacga cgtcaacttg ggttgtgtac ggaacctgcc atcacaaaaa 720aggtgaagca cggagatcta gaagagctgt gacgctcccc tcccattcca ctaggaagct 780gcaaacgcgg tcgcaaacct ggttggaatc aagagaatac acaaagcact tgattagagt 840cgaaaattgg atattcagga accctggctt cgcgttagca gcagctgcca tcgcttggct 900tttgggaagc tcaacgagcc aaaaagtcat atacttggtc atgatactgc tgattgcccc 960ggcatacagc atcaggtgca taggagtcag caatagggac tttgtggaag gtatgtcagg 1020tgggacttgg gttgatgttg tcttggaaca tgggggttgt gtcaccgtaa tggcacagga 1080caaaccgact gtcgacatag agctggttac aacaacagtc agcaacatgg cggaggtaag 1140atcctactgc tatgaggcat caatatcaga catggcttcg gacagccgct gcccaacaca 1200aggtgaagcc taccttgaca agcaatcaga cactcaatat gtctgcaaaa gaacgttagt 1260ggacagaggc tggggaaatg gatgtggact ttttggcaaa gggagcctgg tgacatgcgc 1320taagtttgca tgctccaaga aaatgaccgg gaagagcatc cagccagaga atctggagta 1380ccggataatg ctgtcagttc atggctccca gcacagtggg atgatcgtta atgacacagg 1440acatgaaact gatgagaata gagcgaaggt tgagataacg cccaattcac caagagccga 1500agccaccctg gggggttttg gaagcttagg acttgattgt gaaccgagga caggccttga 1560cttttcagat ttgtattact tgactatgaa taacaagcac tggttggttc acaaggagtg 1620gttccacgac attccattac cttggcacgc tggggcagac accggaactc cacactggaa 1680caacaaagaa gcactggtag agttcaagga cgcacatgcc aaaaggcaaa ctgtcgtggt 1740tctagggact caagaaggag cagttcacac ggcccttgct ggagctctgg aggctgagat 1800ggatggtgca aagggaaggc tgtcctctgg ccacttgaaa tgtcgcctga aaatggataa 1860acttagattg aagggcgtgt catactcctt gtgtaccgca gcgttcacat tcaccaagat 1920cccggctgaa acactgcacg ggacagtcac agtggaggta cagtacgcag ggacagatgg 1980accttgcaag gttccagctc agatggcggt ggacatgcaa actctgaccc cagttgggag 2040gttgataacc gctaaccccg taatcactga aagcactgag aactctaaga tgatgctgga 2100acttgatcca ccatttgggg actcttacat tgtcatagga gtcggggaga agaagatcac 2160ccaccactgg cacaggagtg gcagcaccat tggaaaagca tttgaagcca ctgtgagagg 2220tgccaagaga atggcagtct tgggagacac agcctgggac tttggatcag ttggaggcgc 2280tctcaactca ttgggcaagg gcatccatca aatttttgga gcagctttca aatcattgtt 2340tggaggaatg tcctggttct cacaaattct cattggaacg ttgctgatgt ggttgggtct 2400gaacacaaag aatggatcta tttcccttat gtgcttggcc ttagggggag tgttgatctt 2460cttatccaca gccgtctctg ctgatgtggg gtgctcggtg gacttctcaa agaaggagac 2520gagatgtggt acaggggtgt tcgtctataa cgacgttgaa gcctggaggg acaggtacaa 2580gtaccatcct gactctcccc gtagattggc agcagcagtc aagcaagcct gggaagatgg 2640tatctgcggg atctcctctg tttcaagaat ggaaaacatc atgtggagat cagtagaagg 2700ggagcttaac gcaatcctgg aagagaatgg agttcaactg acggtcgttg tgggatctgt 2760aaaaaacccc atgtggagag gtccacagag attgcccgtg cctgtgaacg agctgcccca 2820cggctggaag gcttggggga aatcgtactt cgtcagagca gcaaagacaa ataacagctt 2880tgtcgtggat ggtgacacac tgaaggaatg cccactcaaa catagagcat ggaacagctt 2940tcttgtggag gatcatgggt tcggggtatt tcacactagt gtctggctca aggttagaga 3000agattattca ttagagtgtg atccagccgt tattggaaca gctgttaagg gaaaggaggc 3060tgtacacagt gatctaggct actggattga gagtgagaag aatgacacat ggaggctgaa 3120gagggcccat ctgatcgaga tgaaaacatg tgaatggcca aagtcccaca cattgtggac 3180agatggaata gaagagagtg atctgatcat acccaagtct ttagctgggc cactcagcca 3240tcacaatacc agagagggct acaggaccca aatgaaaggg ccatggcaca gtgaagagct 3300tgaaattcgg tttgaggaat gcccaggcac taaggtccac gtggaggaaa catgtggaac 3360aagaggacca tctctgagat caaccactgc aagcggaagg gtgatcgagg aatggtgctg 3420cagggagtgc acaatgcccc cactgtcgtt ccgggctaaa gatggctgtt ggtatggaat 3480ggagataagg cccaggaaag aaccagaaag caacttagta aggtcaatgg tgactgcagg 3540atcaactgat cacatggatc acttctccct tggagtgctt gtgattctgc tcatggtgca 3600ggaagggctg aagaagagaa tgaccacaaa gatcatcata agcacatcaa tggcagtgct 3660ggtagctatg atcctgggag gattttcaat gagtgacctg gctaagcttg caattttgat 3720gggtgccacc ttcgcggaaa tgaacactgg aggagatgta gctcatctgg cgctgatagc 3780ggcattcaaa gtcagaccag cgttgctggt atctttcatc ttcagagcta attggacacc 3840ccgtgaaagc atgctgctgg ccttggcctc gtgttttttg caaactgcga tctccgcctt 3900ggaaggcgac ctgatggttc tcatcaatgg ttttgctttg gcctggttgg caatacgagc 3960gatggttgtt ccacgcactg acaacatcac cttggcaatc ctggctgctc tgacaccact 4020ggcccggggc acactgcttg tggcgtggag agcaggcctt gctacttgcg gggggtttat 4080gctcctctct ctgaagggaa

aaggcagtgt gaagaagaac ttaccatttg tcatggccct 4140gggactaacc gctgtgaggc tggtcgaccc catcaacgtg gtgggactgc tgttgctcac 4200aaggagtggg aagcggagct ggccccctag cgaagtactc acagctgttg gcctgatatg 4260cgcattggct ggagggttcg ccaaggcaga tatagagatg gctgggccca tggccgcggt 4320cggtctgcta attgtcagtt acgtggtctc aggaaagagt gtggacatgt acattgaaag 4380agcaggtgac atcacatggg aaaaagatgc ggaagtcact ggaaacagtc cccggctcga 4440tgtggcgcta gatgagagtg gtgacttctc cctggtggag gatgacggtc cccccatgag 4500agagatcata ctcaaggtgg tcctgatgac catctgtggc atgaacccaa tagccatacc 4560ctttgcagct ggagcgtggt acgtatacgt gaagactgga aaaaggagtg gtgctctatg 4620ggatgtgcct gctcccaagg aagtaaaaaa gggggagacc acagatggag tgtacagagt 4680aatgactcgt agactgctag gttcaacaca agttggagtg ggagttatgc aagagggggt 4740ctttcacact atgtggcacg tcacaaaagg atccgcgctg agaagcggtg aagggagact 4800tgatccatac tggggagatg tcaagcagga tctggtgtca tactgtggtc catggaagct 4860agatgccgcc tgggacgggc acagcgaggt gcagctcttg gccgtgcccc ccggagagag 4920agcgaggaac atccagactc tgcccggaat atttaagaca aaggatgggg acattggagc 4980ggttgcgctg gattacccag caggaacttc aggatctcca atcctagaca agtgtgggag 5040agtgatagga ctttatggca atggggtcgt gataaaaaat gggagttatg ttagtgccat 5100cacccaaggg aggagggagg aagagactcc tgttgagtgc ttcgagcctt cgatgctgaa 5160gaagaagcag ctaactgtct tagacttgca tcctggagct gggaaaacca ggagagttct 5220tcctgaaata gtccgtgaag ccataaaaac aagactccgt actgtgatct tagctccaac 5280cagggttgtc gctgctgaaa tggaggaagc ccttagaggg cttccagtgc gttatatgac 5340aacagcagtc aatgtcaccc actctggaac agaaatcgtc gacttaatgt gccatgccac 5400cttcacttca cgtctactac agccaatcag agtccccaac tataatctgt atattatgga 5460tgaggcccac ttcacagatc cctcaagcat agcagcaaga ggatacattt caacaagggt 5520tgagatgggc gaggcggctg ccatcttcat gaccgccacg ccaccaggaa cccgtgacgc 5580atttccggac tccaactcac caattatgga caccgaagtg gaagtcccag agagagcctg 5640gagctcaggc tttgattggg tgacggatca ttctggaaaa acagtttggt ttgttccaag 5700cgtgaggaac ggcaatgaga tcgcagcttg tctgacaaag gctggaaaac gggtcataca 5760gctcagcaga aagacttttg agacagagtt ccagaaaaca aaacatcaag agtgggactt 5820tgtcgtgaca actgacattt cagagatggg cgccaacttt aaagctgacc gtgtcataga 5880ttccaggaga tgcctaaagc cggtcatact tgatggcgag agagtcattc tggctggacc 5940catgcctgtc acacatgcca gcgctgccca gaggaggggg cgcataggca ggaatcccaa 6000caaacctgga gatgagtacc tgtatggagg tgggtgcgca gagactgacg aagaccatgc 6060acactggctt gaagcaagaa tgctccttga caatatttac ctccaagatg gcctcatagc 6120ctcgctctat cgacctgagg ccgacaaagt agcagccatt gagggagagt tcaagcttag 6180gacggagcaa aggaagacct ttgtggaact catgaaaaga ggagatcttc ctgtttggct 6240ggcctatcag gttgcatctg ccggaataac ctacacagat agaagatggt gctttgatgg 6300cacgaccaac aacaccataa tggaagacag tgtgccggca gaggtgtgga ccagacacgg 6360agagaaaaga gtgctcaaac cgaggtggat ggacgccaga gtttgttcag atcatgcggc 6420cctgaagtca ttcaaggagt ttgccgctgg gaaaagagga gcggcttttg gagtgatgga 6480agccctggga acactgccag gacacatgac agagagattc caggaagcca ttgacaacct 6540cgctgtgctc atgcgggcag agactggaag caggccttac aaagccgcgg cggcccaatt 6600gccggagacc ctagagacca ttatgctttt ggggttgctg ggaacagtct cgctgggaat 6660ctttttcgtc ttgatgagga acaagggcat agggaagatg ggctttggaa tggtgactct 6720tggggccagc gcatggctca tgtggctctc ggaaattgag ccagccagaa ttgcatgtgt 6780cctcattgtt gtgttcctat tgctggtggt gctcatacct gagccagaaa agcaaagatc 6840tccccaggac aaccaaatgg caatcatcat catggtagca gtaggtcttc tgggcttgat 6900taccgccaat gaactcggat ggttggagag aacaaagagt gacctaagcc atctaatggg 6960aaggagagag gagggggcaa ccataggatt ctcaatggac attgacctgc ggccagcctc 7020agcttgggcc atctatgctg ccttgacaac tttcattacc ccagccgtcc aacatgcagt 7080gaccacttca tacaacaact actccttaat ggcgatggcc acgcaagctg gagtgttgtt 7140tggtatgggc aaagggatgc cattctacgc atgggacttt ggagtcccgc tgctaatgat 7200aggttgctac tcacaattaa cacccctgac cctaatagtg gccatcattt tgctcgtggc 7260gcactacatg tacttgatcc cagggctgca ggcagcagct gcgcgtgctg cccagaagag 7320aacggcagct ggcatcatga agaaccctgt tgtggatgga atagtggtga ctgacattga 7380cacaatgaca attgaccccc aagtggagaa aaagatggga caggtgctac tcatagcagt 7440agccgtctcc agcgccatac tgtcgcggac cgcctggggg tggggggagg ctggggccct 7500gatcacagcc gcaacttcca ctttgtggga aggctctccg aacaagtact ggaactcctc 7560tacagccact tcactgtgta acatttttag gggaagttac ttggctggag cttctctaat 7620ctacacagta acaagaaacg ctggcttggt caagagacgt gggggtggaa caggagagac 7680cctgggagag aaatggaagg cccgcttgaa ccagatgtcg gccctggagt tctactccta 7740caaaaagtca ggcatcaccg aggtgtgcag agaagaggcc cgccgcgccc tcaaggacgg 7800tgtggcaacg ggaggccatg ctgtgtcccg aggaagtgca aagctgagat ggttggtgga 7860gcggggatac ctgcagccct atggaaaggt cattgatctt ggatgtggca gagggggctg 7920gagttactac gccgccacca tccgcaaagt tcaagaagtg aaaggataca caaaaggagg 7980ccctggtcat gaagaacccg tgttggtgca aagctatggg tggaacatag tccgtcttaa 8040gagtggggtg gacgtctttc atatggcggc tgagccgtgt gacacgttgc tgtgtgacat 8100aggtgagtca tcatctagtc ctgaagtgga agaagcacgg acgctcagag tcctctccat 8160ggtgggggat tggcttgaaa aaagaccagg agccttttgc ataaaagtgt tgtgcccata 8220caccagcact atgatggaaa ccctggagcg actgcagcgt aggtatgggg gaggactggt 8280cagagtgcca ctctcccgca actctacaca tgagatgtac tgggtctctg gagcgaaaag 8340caacaccata aaaagtgtgt ccaccacgag ccagctcctc ttggggcgca tggacgggcc 8400taggaggcca gtgaaatatg aggaggatgt gaatctcggc tctggcacgc gggctgtggt 8460aagctgcgct gaagctccca acatgaagat cattggtaac cgcattgaaa ggatccgcag 8520tgagcacgcg gaaacgtggt tctttgacga aaaccaccca tataggacat gggcttacca 8580tggaagctat gtggccccca cacaagggtc agcgtcctct ctaataaacg gggttgtcag 8640gctcctgtca aaaccctggg atgtggtgac tggagtcaca ggaatagcca tgaccgacac 8700cacaccgtat ggtcagcaaa gagttttcaa ggaaaaagtg gacactaggg tgccagaccc 8760ccaagaaggc actcgtcagg ttatgagcat ggtctcttcc tggttgtgga aagagctagg 8820caaacacaaa cgaccacgag tctgtaccaa agaagagttc atcaacaagg ttcgtagcaa 8880tgcagcatta ggggcaatat ttgaagagga aaaagagtgg aagactgcag tggaagctgt 8940gaacgatcca aggttctggg ctctagtgga caaggaaaga gagcaccacc tgagaggaga 9000gtgccagagt tgtgtgtaca acatgatggg aaaaagagaa aagaaacaag gggaatttgg 9060aaaggccaag ggcagccgcg ccatctggta tatgtggcta ggggctagat ttctagagtt 9120cgaagccctt ggattcttga acgaggatca ctggatgggg agagagaact caggaggtgg 9180tgttgaaggg ctgggattac aaagactcgg atatgtccta gaagagatga gtcgcatacc 9240aggaggaagg atgtatgcag atgacactgc tggctgggac acccgcatca gcaggtttga 9300tctggagaat gaagctctaa tcaccaacca aatggagaaa gggcacaggg ccttggcatt 9360ggccataatc aagtacacat accaaaacaa agtggtaaag gtccttagac cagctgaaaa 9420agggaaaaca gttatggaca ttatttcgag acaagaccaa agggggagcg gacaagttgt 9480cacttacgct cttaacacat ttaccaacct agtggtgcaa ctcattcgga atatggaggc 9540tgaggaagtt ctagagatgc aagacttgtg gctgctgcgg aggtcagaga aagtgaccaa 9600ctggttgcag agcaacggat gggataggct caaacgaatg gcagtcagtg gagatgattg 9660cgttgtgaag ccaattgatg ataggtttgc acatgccctc aggttcttga atgatatggg 9720aaaagttagg aaggacacac aagagtggaa accctcaact ggatgggaca actgggaaga 9780agttccgttt tgctcccacc acttcaacaa gctccatctc aaggacggga ggtccattgt 9840ggttccctgc cgccaccaag atgaactgat tggccgggcc cgcgtctctc caggggcggg 9900atggagcatc cgggagactg cttgcctagc aaaatcatat gcgcaaatgt ggcagctcct 9960ttatttccac agaagggacc tccgactgat ggccaatgcc atttgttcat ctgtgccagt 10020tgactgggtt ccaactggga gaactacctg gtcaatccat ggaaagggag aatggatgac 10080cactgaagac atgcttgtgg tgtggaacag agtgtggatt gaggagaacg accacatgga 10140agacaagacc ccagttacga aatggacaga cattccctat ttgggaaaaa gggaagactt 10200gtggtgtgga tctctcatag ggcacagacc gcgcaccacc tgggctgaga acattaaaaa 10260tacagtcaac atggtgcgca ggatcatagg tgatgaagaa aagtacatgg actacctatc 10320cacccaagtt cgctacttgg gtgaagaagg gtctacacct ggagtgctgt gagcaccaat 10380cttaatgttg tcaggcctgc tagtcagcca cagcttgggg aaagctgtgc agcctgtgac 10440ccctccagga gaagctgggt aaccaagcct atagtcaggc cgagaacgcc atggcacgga 10500agaagccatg ctgcctgtga gcccctcaga ggacactgag tcaaaaaacc ccacgcgctt 10560ggaggcgcag gatgggaaaa gaaggtggcg accttcccca cccttcaatc tggggcctga 10620actggagatc agctgtggat ctccagaaga gggactagtg gttagaggag accccccgga 10680aaacgcaaaa cagcatattg acgctgggaa agaccagaga ctccatgagt ttccaccacg 10740ctggccgcca ggcacagatc gccgaatagc ggcggccggt gtggggaaat cca 10793410675DNAZika virus 4gttgttgatc tgtgtgaatc agactgcgac agttcgagtt tgaagcgaaa gctagcaaca 60gtatcaacag gttttatttt ggatttggaa acgagagttt ctggtcatga aaaacccaaa 120aaagaaatcc ggaggattcc ggattgtcaa tatgctaaaa cgcggagtag cccgtgtgag 180cccctttggg ggcttgaaga ggctgccagc cggacttctg ctgggtcatg ggcccatcag 240gatggtcttg gcgattctag cctttttgag attcacggca atcaagccat cactgggtct 300catcaataga tggggttcag tggggaaaaa agaggctatg gaaacaataa agaagttcaa 360gaaagatctg gctgccatgc tgagaataat caatgctagg aaggagaaga agagacgagg 420cgcagatact agtgtcggaa ttgttggcct cctgctgacc acagctatgg cagcggaggt 480cactagacgt gggagtgcat actatatgta cttggacaga aacgatgctg gggaggccat 540atcttttcca accacattgg ggatgaataa gtgttatata cagatcatgg atcttggaca 600catgtgtgat gccaccatga gctatgaatg ccctatgctg gatgaggggg tggaaccaga 660tgacgtcgat tgttggtgca acacgacgtc aacttgggtt gtgtacggaa cctgccatca 720caaaaaaggt gaagcacgga gatctagaag agctgtgacg ctcccctccc attccaccag 780gaagctgcaa acgcggtcgc aaacctggtt ggaatcaaga gaatacacaa agcacttgat 840tagagtcgaa aattggatat tcaggaaccc tggcttcgcg ttagcagcag ctgccatcgc 900ttggcttttg ggaagctcaa cgagccaaaa agtcatatac ttggtcatga tactgctgat 960tgccccggca tacagcatca ggtgcatagg agtcagcaat agggactttg tggaaggtat 1020gtcaggtggg acttgggttg atgttgtctt ggaacatgga ggttgtgtca ccgtaatggc 1080acaggacaaa ccgactgtcg acatagagct ggttacaaca acagtcagca acatggcgga 1140ggtaagatcc tactgctatg aggcatcaat atcagacatg gcttctgaca gccgctgccc 1200aacacaaggt gaagcctacc ttgacaagca atcagacact caatatgtct gcaaaagaac 1260gttagtggac agaggctggg gaaatggatg tggacttttt ggcaaaggga gcctggtgac 1320atgcgctaag tttgcatgct ccaagaaaat gaccgggaag agcatccagc cagagaatct 1380ggagtaccgg ataatgctgt cagttcatgg ctcccagcac agtgggatga tcgttaatga 1440cacaggacat gaaactgatg agaatagagc gaaagttgag ataacgccca attcaccgag 1500agccgaagcc accctggggg gttttggaag cctaggactt gattgtgaac cgaggacagg 1560ccttgacttt tcagatttgt attacttgac tatgaataac aagcactggt tggttcacaa 1620ggagtggttc cacgacattc cattaccttg gcacgctggg gcagacaccg gaactccaca 1680ctggaacaac aaagaagcac tggtagagtt caaggacgca catgccaaaa ggcaaactgt 1740cgtggttcta gggagtcaag aaggagcagt tcacacggcc cttgctggag ctctggaggc 1800tgagatggat ggtgcaaagg gaaggctgtc ctctggccac ttgaaatgtc gcctgaaaat 1860ggataaactt agattgaagg gcgtgtcata ctccttgtgt actgcagcgt tcacattcac 1920caagatcccg gctgaaacac tgcacgggac agtcacagtg gaggtacagt acgcagggac 1980agatggacct tgcaaggttc cagctcagat ggcggtggac atgcaaactc tgaccccagt 2040tgggaggttg ataaccgcta accccgtaat cactgaaagc actgagaact ctaagatgat 2100gctggaactt gatccaccat ttggggactc ttacattgtc ataggagtcg gggagaagaa 2160gatcacccac cactggcaca ggagtggcag caccattgga aaagcatttg aagccactgt 2220gagaggtgcc aagagaatgg cagtcttggg agacacagcc tgggactttg gatcagttgg 2280aggcgctctc aactcattgg gcaagggcat ccatcaaatt tttggagcag ctttcaaatc 2340attgtttgga ggaatgtcct ggttctcaca aattctcatt ggaacgttgc tgatgtggtt 2400gggtctgaac acaaagaatg gatctatttc ccttatgtgc ttggccttag ggggagtgtt 2460gatcttctta tccacagccg tctctgctga tgtggggtgc tcggtggact tctcaaagaa 2520ggagacgaga tgcggtacag gggtgttcgt ctataacgac gttgaagcct ggagggacag 2580gtacaagtac catcctgact ccccccgtag attggcagca gcagtcaagc aagcctggga 2640agatggtatc tgcgggatct cctctgtttc aagaatggaa aacatcatgt ggagatcagt 2700agaaggggag ctcaacgcaa tcctggaaga gaatggagtt caactgacgg tcgttgtggg 2760atctgtaaaa aaccccatgt ggagaggtcc acagagattg cccgtgcctg tgaacgagct 2820gccccacggc tggaaggctt gggggaaatc gtatttcgtc agagcagcaa agacaaataa 2880cagctttgtc gtggatggtg acacactgaa ggaatgccca ctcaaacata gagcatggaa 2940cagctttctt gtggaggatc atgggttcgg ggtatttcac actagtgtct ggctcaaggt 3000tagagaagat tattcattag agtgtgatcc agccgttatt ggaacagctg ttaagggaaa 3060ggaggctgta cacagtgatc taggctactg gattgagagt gagaagaatg acacatggag 3120gctgaagagg gcccatctga tcgagatgaa aacatgtgaa tggccaaagt cccacacatt 3180gtggacagat ggaatagaag agagtgatct gatcataccc aagtctttag ctgggccact 3240cagccatcac aataccagag agggctacag gacccaaatg aaagggccat ggcacagtga 3300agagcttgaa attcggtttg aggaatgccc aggcactaag gtccacgtgg aggaaacatg 3360tggaacaaga ggaccatctc tgagatcaac cactgcaagc ggaagggtga tcgaggaatg 3420gtgctgcagg gagtgcacaa tgcccccact gtcgttccgg gctaaagatg gctgttggta 3480tggaatggag ataaggccca ggaaagaacc agaaagcaac ttagtaaggt caatggtgac 3540tgcaggatca actgatcaca tggaccactt ctcccttgga gtgcttgtga tcctgctcat 3600ggtgcaggaa gggctgaaga agagaatgac cacaaagatc atcataagca catcaatggc 3660agtgctggta gctatgatcc tgggaggatt ttcaatgagt gacctggcta agcttgcaat 3720tttgatgggt gccaccttcg cggaaatgaa cactggagga gatgtagctc atctggcgct 3780gatagcggca ttcaaagtca gaccagcgtt gctggtatct ttcatcttca gagctaattg 3840gacaccccgt gaaagcatgc tgctggcctt ggcctcgtgt cttttgcaaa ctgcgatctc 3900cgccttggaa ggcgacctga tggttctcat caatggtttt gctttggcct ggttggcaat 3960acgagcgatg gttgttccac gcactgataa catcaccttg gcaatcctgg ctgctctgac 4020accactggcc cggggcacac tgcttgtggc gtggagagca ggccttgcta cttgcggggg 4080gtttatgctc ctctctctga agggaaaagg cagtgtgaag aagaacttac catttgtcat 4140ggccctggga ctaaccgctg tgaggctggt cgaccccatc aacgtggtgg gactgctgtt 4200gctcacaagg agtgggaagc ggagctggcc ccctagcgaa gtactcacag ctgttggcct 4260gatatgcgca ttggctggag ggttcgccaa ggcagatata gagatggctg ggcccatggc 4320cgcggtcggt ctgctaattg tcagttacgt ggtctcagga aagagtgtgg acatgtacat 4380tgaaagagca ggtgacatca catgggaaaa agatgcggaa gtcactggaa acagtccccg 4440gctcgatgtg gcgctagatg agagtggtga tttctccctg gtggaggatg acggtccccc 4500catgagagag atcatactca aggtggtcct gatgaccatc tgtggcatga acccaatagc 4560catacccttt gcagctggag cgtggtacgt atacgtgaag actggaaaaa ggagtggtgc 4620tctatgggat gtgcctgctc ccaaggaagt aaaaaagggg gagaccacag atggagtgta 4680cagagtaatg actcgtagac tgctaggttc aacacaagtt ggagtgggag ttatgcaaga 4740gggggtcttt cacactatgt ggcacgtcac aaaaggatcc gcgctgagaa gcggtgaagg 4800gagacttgat ccatactggg gagatgtcaa gcaggatctg gtgtcatact gtggtccatg 4860gaagctagat gccgcctggg atgggcacag cgaggtgcag ctcttggccg tgccccccgg 4920agagagagcg aggaacatcc agactctgcc cggaatattt aagacaaagg atggggacat 4980tggagcggtt gcgctggatt acccagcagg aacttcagga tctccaatcc tagacaagtg 5040tgggagagtg ataggacttt atggcaatgg ggtcgtgatc aaaaacggga gttatgttag 5100tgccatcacc caagggagga gggaggaaga gactcctgtt gagtgcttcg agccctcgat 5160gctgaagaag aagcagctaa ctgtcttaga cttgcatcct ggagctggga aaaccaggag 5220agttcttcct gaaatagtcc gtgaagccat aaaaacaaga ctccgtactg tgatcttagc 5280tccaaccagg gttgtcgctg ctgaaatgga ggaggccctt agagggcttc cagtgcgtta 5340tatgacaaca gcagtcaatg tcacccactc tggaacagaa atcgtcgact taatgtgcca 5400tgccaccttc acttcacgtc tactacagcc aatcagagtc cccaactata atctgtatat 5460tatggatgag gcccacttca cagatccctc aagtatagca gcaagaggat acatttcaac 5520aagggttgag atgggcgagg cggctgccat cttcatgacc gccacgccac caggaacccg 5580tgacgcattt ccggactcca actcaccaat tatggacacc gaagtggaag tcccagagag 5640agcctggagc tcaggctttg attgggtgac ggatcattct ggaaaaacag tttggtttgt 5700tccaagcgtg aggaacggca atgagatcgc agcttgtctg acaaaggctg gaaaacgggt 5760catacagctc agcagaaaga cttttgagac agagttccag aaaacaaaac atcaagagtg 5820ggactttgtc gtgacaactg acatttcaga gatgggcgcc aactttaaag ctgaccgtgt 5880catagattcc aggagatgcc taaagccggt catacttgat ggcgagagag tcattctggc 5940tggacccatg cctgtcacac atgccagcgc tgcccagagg agggggcgca taggcaggaa 6000tcccaacaaa cctggagatg agtatctgta tggaggtggg tgcgcagaga ctgacgaaga 6060ccatgcacac tggcttgaag caagaatgct ccttgacaat atttacctcc aagatggcct 6120catagcctcg ctctatcgac ctgaggccga caaagtagca gccattgagg gagagttcaa 6180gcttaggacg gagcaaagga agacctttgt ggaactcatg aaaagaggag atcttcctgt 6240ttggctggcc tatcaggttg catctgccgg aataacctac acagatagaa gatggtgctt 6300tgatggcacg accaacaaca ccataatgga agacagtgtg ccggcagagg tgtggaccag 6360acacggagag aaaagagtgc tcaaaccgag gtggatggac gccagagttt gttcagatca 6420tgcggccctg aagtcattca aggagtttgc cgctgggaaa agaggagcgg cttttggagt 6480gatggaagcc ctgggaacac tgccaggaca catgacagag agattccagg aagccattga 6540caacctcgct gtgctcatgc gggcagagac tggaagcagg ccttacaaag ccgcggcggc 6600ccaattgccg gagaccctag agaccataat gcttttgggg ttgctgggaa cagtctcgct 6660gggaatcttc ttcgtcttga tgaggaacaa gggcataggg aagatgggct ttggaatggt 6720gactcttggg gccagcgcat ggctcatgtg gctctcggaa attgagccag ccagaattgc 6780atgtgtcctc attgttgtgt tcctattgct ggtggtgctc atacctgagc cagaaaagca 6840aagatctccc caggacaacc aaatggcaat catcatcatg gtagcagtag gtcttctggg 6900cttgattacc gccaatgaac tcggatggtt ggagagaaca aagagtgacc taagccatct 6960aatgggaagg agagaggagg gggcaaccat aggattctca atggacattg acctgcggcc 7020agcctcagct tgggccatct atgctgcctt gacaactttc attaccccag ccgtccaaca 7080tgcagtgacc acctcataca acaactactc cttaatggcg atggccacgc aagctggagt 7140gttgtttggc atgggcaaag ggatgccatt ctacgcatgg gactttggag tcccgctgct 7200aatgataggt tgctactcac aattaacacc cctgacccta atagtggcca tcattttgct 7260cgtggcgcac tacatgtact tgatcccagg gctgcaggca gcagctgcgc gtgctgccca 7320gaagagaacg gcagctggca tcatgaagaa ccctgttgtg gatggaatag tggtgactga 7380cattgacaca atgacaattg acccccaagt ggagaaaaag atgggacagg tgctactcat 7440agcagtagcc gtctccagcg ccatactgtc gcggaccgcc tgggggtggg gggaggctgg 7500ggctctgatc acagccgcaa cttccacttt gtgggaaggc tctccgaaca agtactggaa 7560ctcctctaca gccacttcac tgtgtaacat ttttagggga agttacttgg ctggagcttc 7620tctaatctac acagtaacaa gaaacgctgg cttggtcaag agacgtgggg gtggaacagg 7680agagaccctg ggagagaaat ggaaggcccg cttgaaccag atgtcggccc tggagttcta 7740ctcctacaaa aagtcaggca tcaccgaggt gtgcagagaa gaggcccgcc gcgccctcaa 7800ggacggtgtg gcaacgggag gccatgctgt gtcccgagga agtgcaaagc tgagatggtt 7860ggtggagcgg ggatacctgc agccctatgg aaaggtcatt gatcttggat gtggcagagg 7920gggctggagt tactacgtcg ccaccatccg caaagttcaa gaagtgaaag gatacacaaa 7980aggaggccct ggtcatgaag aacccgtgtt ggtgcaaagc tatgggtgga acatagtccg 8040tcttaagagt ggggtggacg tctttcatat ggcggctgag ccgtgtgaca cgttgctgtg 8100tgacataggt gagtcatcat ctagtcctga agtggaagaa gcacggacgc tcagagtcct 8160ctccatggtg ggggattggc ttgaaaaaag accaggagcc ttttgtataa aagtgttgtg 8220cccatacacc agcactatga tggaaaccct ggagcgactg cagcgtaggt atgggggagg 8280actggtcaga gtgccactct cccgcaactc tacacatgag atgtactggg tctctggagc

8340gaaaagcaac accataaaaa gtgtgtccac cacgagccag ctcctcttgg ggcgcatgga 8400cgggcctagg aggccagtga aatatgagga ggatgtgaat ctcggctctg gcacgcgggc 8460tgtggtaagc tgcgctgaag ctcccaacat gaagatcatt ggtaaccgca ttgaaaggat 8520ccgcagtgag cacgcggaaa cgtggttctt tgacgagaac cacccatata ggacatgggc 8580ttaccatgga agctatgagg cccccacaca agggtcagcg tcctctctaa taaacggggt 8640tgtcaggctc ctgtcaaaac cctgggatgt ggtgactgga gtcacaggaa tagccatgac 8700cgacaccaca ccgtatggtc agcaaagagt tttcaaggaa aaagtggaca ctagggtgcc 8760agacccccaa gaaggcactc gtcaggttat gagcatggtc tcttcctggt tgtggaaaga 8820gctaggcaaa cacaaacggc cacgagtctg caccaaagaa gagttcatca acaaggttcg 8880tagcaatgca gcattagggg caatatttga agaggaaaaa gagtggaaga ctgcagtgga 8940agctgtgaac gatccaaggt tctgggctct agtggacaag gaaagagagc accacctgag 9000aggagagtgc cagagctgtg tgtacaacat gatgggaaaa agagaaaaga aacaagggga 9060atttggaaag gccaagggca gccgcgccat ctggtatatg tggctagggg ctagatttct 9120agagttcgaa gcccttggat tcttgaacga ggatcactgg atggggagag agaactcagg 9180aggtggtgtt gaagggctgg gattacaaag actcggatat gtcctagaag agatgagtcg 9240tataccagga ggaaggatgt atgcagatga cactgctggc tgggacaccc gcattagcag 9300gtttgatctg gagaatgaag ctctaatcac caaccaaatg gagaaagggc acagggcctt 9360ggcattggcc ataatcaagt acacatacca aaacaaagtg gtaaaggtcc ttagaccagc 9420tgaaaaaggg aaaacagtta tggacattat ttcgagacaa gaccaaaggg ggagcggaca 9480agttgtcact tacgctctta acacatttac caacctagtg gtgcaactca ttcggaatat 9540ggaggctgag gaagttctag agatgcaaga cttgtggctg ctgcggaggt cagagaaagt 9600gaccaactgg ttgcagagca acggatggga taggctcaaa cgaatggcag tcagtggaga 9660tgattgcgtt gtgaagccaa ttgatgatag gtttgcacat gccctcaggt tcttgaatga 9720tatgggaaaa gttaggaagg acacacaaga gtggaaaccc tcaactggat gggacaactg 9780ggaagaagtt ccgttttgct cccaccactt caacaagctc catctcaagg acgggaggtc 9840cattgtggtt ccctgccgcc accaagatga actgattggc cgggcccgcg tctctccagg 9900ggcgggatgg agcatccggg agactgcttg cctagcaaaa tcatatgcgc aaatgtggca 9960gctcctttat ttccacagaa gggacctccg actgatggcc aatgccattt gttcatctgt 10020gccagttgac tgggttccaa ctgggagaac tacctggtca atccatggaa agggagaatg 10080gatgaccact gaagacatgc ttgtggtgtg gaacagagtg tggattgagg agaacgacca 10140catggaagac aagaccccag ttacgaaatg gacagacatt ccctatttgg gaaaaaggga 10200agacttgtgg tgtggatctc tcatagggca cagaccgcgc accacctggg ctgagaacat 10260taaaaacaca gtcaacatgg tgcgcaggat cataggtgat gaagaaaagt acatggacta 10320cctatccacc caagttcgct acttgggtga agaagggtct acacctggag tgctgtaagc 10380accaatctta atgttgtcag gcctgctagt cagccacagc ttggggaaag ctgtgcagcc 10440tgtgaccccc ccaggagaag ctgggaaacc aagcctatag tcaggccgag aacgccatgg 10500cacggaagaa gccatgctgc ctgtgagccc ctcagaggac actgagtcaa aaaaccccac 10560gcgcttggag gcgcaggatg ggaaaagaag gtggcgacct tccccaccct tcaatctggg 10620gcctgaactg gagatcagct gtggatctcc agaagaggga ctagtggtta gagga 10675510676DNAZika virus 5gttgttactg ttgctgactc agactgcgac agttcgagtt tgaagcgaaa gctagcaaca 60gtatcaacag gttttatttg gatttggaaa cgagagtttc tggtcatgaa aaacccaaaa 120aagaaatccg gaggattccg gattgtcaat atgctaaaac gcggagtagc ccgtgtgagc 180ccctttgggg gcttgaagag gctgccagcc ggacttctgc tgggtcatgg gcccatcagg 240atggtcttgg caattctagc ctttttgaga ttcacggcaa tcaagccatc actgggtctc 300atcaatagat ggggttcagt ggggaaaaaa gaggctatgg aaataataaa gaagttcaag 360aaagatctgg ctgccatgct gagaataatc aatgctagga aggagaagaa gagacgaggc 420gcagatacta gtgtcggaat tgttggcctc ctgctgacca cagctatggc agcggaggtc 480actagacgtg ggagtgcata ctatatgtac ttggacagaa acgatgctgg ggaggccata 540tcttttccaa ccacattggg gatgaataag tgttatatac agatcatgga tcttggacac 600atgtgtgatg ccaccatgag ctatgaatgc cctatgctgg atgagggggt ggaaccagat 660gacgtcgatt gttggtgcaa cacgacgtca acttgggttg tgtacggaac ctgccatcac 720aaaaaaggtg aagcacggag atctagaaga gctgtgacgc tcccctccca ttccactagg 780aagctgcaaa cgcggtcgca aacctggttg gaatcaagag aatacacaaa gcacttgatt 840agagtcgaaa attggatatt caggaaccct ggcttcgcgt tagcagcagc tgccatcgct 900tggcttttgg gaagctcaac gagccaaaaa gtcatatact tggtcatgat actgctgatt 960gccccggcat acagcatcag gtgcatagga gtcagcaata gggactttgt ggaaggtatg 1020tcaggtggga cttgggttga tgttgtcttg gaacatggag gttgtgtcac cgtaatggca 1080caggacaaac cgactgtcga catagagctg gttacaacaa cagtcagcaa catggcggag 1140gtaagatcct actgctatga ggcatcaata tcagacatgg cttcggacag ccgctgccca 1200acacaaggtg aagcctacct tgacaagcaa tcagacactc aatatgtctg caaaagaacg 1260ttagtggaca gaggctgggg aaatggatgt ggactttttg gcaaagggag tctggtgaca 1320tgcgctaagt ttgcatgctc caagaaaatg accgggaaga gcatccagcc agagaatctg 1380gagtaccgga taatgctgtc agttcatggc tcccagcaca gtgggatgat cgttaatgac 1440acaggacatg aaactgatga gaatagagcg aaggttgaga taacgcccaa ttcaccaaga 1500gccgaagcca ccctgggggg ttttggaagc ctaggacttg attgtgaacc gaggacaggc 1560cttgactttt cagatttgta ttacttgact atgaataaca agcactggtt ggttcacaag 1620gagtggttcc acgacattcc attaccttgg cacgctgggg cagacaccgg aactccacac 1680tggaacaaca aagaagcact ggtagagttc aaggacgcac atgccaaaag gcaaactgtc 1740gtggttctag ggagtcaaga aggagcagtt cacacggccc ttgctggagc tctggaggct 1800gagatggatg gtgcaaaggg aaggctgtcc tctggccact tgaaatgtcg cctgaaaatg 1860gataaactta gattgaaggg cgtgtcatac tccttgtgta ccgcagcgtt cacattcacc 1920aagatcccgg ctgaaacact gcacgggaca gtcacagtgg aggtacagta cgcagggaca 1980gatggacctt gcaaggttcc agctcagatg gcggtggaca tgcaaactct gaccccagtt 2040gggaggttga taaccgctaa ccccgtaatc actgaaagca ctgagaactc taagatgatg 2100ctggaacttg atccaccatt tggggactct tacattgtca taggagtcgg ggagaagaag 2160atcacccacc actggcacag gagtggcagc accattggaa aagcatttga agccactgtg 2220agaggtgcca agagaatggc agtcttggga gacacagcct gggactttgg atcagttgga 2280ggcgctctca actcattggg caagggcatc catcaaattt ttggagcagc tttcaaatca 2340ttgtttggag gaatgtcctg gttctcacaa attctcattg gaacgttgct gatgtggttg 2400ggtctgaaca caaagaatgg atctatttcc cttatgtgct tggccttagg gggagtgttg 2460atcttcttat ccacagccgt ctctgctgat gtggggtgct cggtggactt ctcaaagaag 2520gagacgagat gcggtacagg ggtgttcgtc tataacgacg ttgaagcctg gagggacagg 2580tacaagtacc atcctgactc cccccgtaga ttggcagcag cagtcaagca agcctgggaa 2640gatggtatct gcgggatctc ctctgtttca agaatggaaa acatcatgtg gagatcagta 2700gaaggggagc tcaacgcaat cctggaagag aatggagttc aactgacggt cgttgtggga 2760tctgtaaaaa accccatgtg gagaggtcca cagagattgc ccgtgcctgt gaacgagctg 2820ccccacggct ggaaggcttg ggggaaatcg cacttcgtca gagcagcaaa gacaaataac 2880agctttgtcg tggatggtga cacactgaag gaatgcccac tcaaacatag agcatggaac 2940agctttcttg tggaggatca tgggttcggg gtatttcaca ctagtgtctg gctcaaggtt 3000agagaagatt attcattaga gtgtgatcca gccgttattg gaacagctgt taagggaaag 3060gaggctgtac acagtgatct aggctactgg attgagagtg agaagaatga cacatggagg 3120ctgaagaggg cccatctgat cgagatgaaa acatgtgaat ggccaaagtc ccacacattg 3180tggacagatg gaatagaaga gagtgatctg atcataccca agtctttagc tgggccactc 3240agccatcaca ataccagaga gggctacagg acccaaatga aagggccatg gcacagtgaa 3300gagcttgaaa ttcggtttga ggaatgccca ggcactaagg tccacgtgga ggaaacatgt 3360ggaacaagag gaccatctct gagatcaacc actgcaagcg gaagggtgat cgaggaatgg 3420tgctgcaggg agtgcacaat gcccccactg tcgttccggg ctaaagatgg ctgttggtat 3480ggaatggaga taaggcccag gaaagaacca gaaagcaact tagtaaggtc aatggtgact 3540gcaggatcaa ctgatcacat ggatcacttc tcccttggag tgcttgtgat tctgctcatg 3600gtgcaggaag ggctgaagaa gagaatgacc acaaagatca tcataagcac atcaatggca 3660gtgctggtag ctatgatcct gggaggattt tcaatgagtg acctggctaa gcttgcaatt 3720ttgatgggtg ccaccttcgc ggaaatgaac actggaggag atgtagctca tctggcgctg 3780atagcggcat tcaaagtcag accagcgttg ctggtatctt tcatcttcag agctaattgg 3840acaccccgtg aaagcatgct gctggccttg gcctcgtgtc ttttgcaaac tgcgatctcc 3900gccttggaag gcgacctgat ggttctcatc aatggttttg ctttggcctg gttggcaata 3960cgagcgatgg ttgttccacg cactgataac atcaccttgg caatcctggc tgctctgaca 4020ccactggccc ggggcacact gcttgtggcg tggagagcag gccttgctac ttgcgggggg 4080tttatgctcc tctctctgaa gggaaaaggc agtgtgaaga agaacttacc atttgtcatg 4140gccctgggac taaccgctgt gaggctggtc gaccccatca acgtggtggg gctgctgttg 4200ctcacaagga gtgggaagcg gagctggccc cctagcgaag tactcacagc tgttggcctg 4260atatgcgcat tggctggagg gttcgccaag gcagatatag agatggctgg gcccatggcc 4320gcggtcggtc tgctaattgt cagttacgtg gtctcaggaa agagtgtgga catgtacatt 4380gaaagagcag gtgacatcac atgggaaaaa gatgcggaag tcactggaaa cagtccccgg 4440ctcgatgtgg cgctagatga gagtggtgat ttctccctgg tggaggatga cggtcccccc 4500atgagagaga tcatactcaa ggtggtcctg atgaccatct gtggcatgaa cccaatagcc 4560ataccctttg cagctggagc gtggtacgta tacgtgaaga ctggaaaaag gagtggtgct 4620ctatgggatg tgcctgctcc caaggaagta aaaaaggggg agaccacaga tggagtgtac 4680agagtaatga ctcgtagact gctaggttca acacaagttg gagtgggagt tatgcaagag 4740ggggtctttc acactatgtg gcacgtcaca aaaggatccg cgctgagaag cggtgaaggg 4800agacttgatc catactgggg agatgtcaag caggatctgg tgtcatactg tggtccatgg 4860aagctagatg ccgcctggga cgggcacagc gaggtgcagc tcttggccgt gccccccgga 4920gagagagcga ggaacatcca gactctgccc ggaatattta agacaaagga tggggacatt 4980ggagcggttg cgctggatta cccagcagga acttcaggat ctccaatcct agacaagtgt 5040gggagagtga taggacttta tggcaatggg gtcgtgatca aaaatgggag ttatgttagt 5100gccatcaccc aagggaggag ggaggaagag actcctgttg agtgcttcga gccttcgatg 5160ctgaagaaga agcagctaac tgtcttagac ttgcatcctg gagctgggaa aaccaggaga 5220gttcttcctg aaatagtccg tgaagccata aaaacaagac tccgtactgt gatcttagct 5280ccaaccaggg ttgtcgctgc tgaaatggag gaagccctta gagggcttcc agtgcgttat 5340atgacaacag cagtcaatgt cacccactct ggaacagaaa tcgtcgactt aatgtgccat 5400gccaccttca cttcacgtct actacagcca atcagagtcc ccaactataa tctgtatatt 5460atggatgagg cccacttcac agatccctca agtatagcag caagaggata catttcaaca 5520agggttgaga tgggcgaggc ggctgccatc ttcatgaccg ccacgccacc aggaacccgt 5580gacgcatttc cggactccaa ctcaccaatt atggacaccg aagtggaagt cccagagaga 5640gcctggagct caggctttga ttgggtgacg gattattctg gaaaaacagt ttggtttgtt 5700ccaagcgtga ggaacggcaa tgagatcgca gcttgtctga caaaggctgg aaaacgggtc 5760atacagctca gcagaaagac ttttgagaca gagttccaga aaacaaaaca tcaagagtgg 5820gactttgtcg tgacaactga catttcagag atgggcgcca actttaaagc tgaccgtgtc 5880atagattcca ggagatgcct aaagccggtc atacttgatg gcgagagagt cattctggct 5940ggacccatgc ctgtcacaca tgccagcgct gcccagagga gggggcgcat aggcaggaat 6000cccaacaaac ctggagatga gtatctgtat ggaggtgggt gcgcagagac tgacgaagac 6060catgcacact ggcttgaagc aagaatgctc cttgacaata tttacctcca agatggcctc 6120atagcctcgc tctatcgacc tgaggccgac aaagtagcag ccattgaggg agagttcaag 6180cttaggacgg agcaaaggaa gacctttgtg gaactcatga aaagaggaga tcttcctgtt 6240tggctggcct atcaggttgc atctgccgga ataacctaca cagatagaag atggtgcttt 6300gatggcacga ccaacaacac cataatggaa gacagtgtgc cggcagaggt gtggaccaga 6360cacggagaga aaagagtgct caaaccgagg tggatggacg ccagagtttg ttcagatcat 6420gcggccctga agtcattcaa ggagtttgcc gctgggaaaa gaggagcggc ttttggagtg 6480atggaagccc tgggaacact gccaggacac atgacagaga gattccagga agccattgac 6540aacctcgctg tgctcatgcg ggcagagact ggaagcaggc cttacaaagc cgcggcggcc 6600caattgccgg agaccctaga gaccattatg cttttggggt tgctgggaac agtctcgctg 6660ggaatctttt tcgtcttgat gaggaacaag ggcataggga agatgggctt tggaatggtg 6720actcttgggg ccagcgcatg gctcatgtgg ctctcggaaa ttgagccagc cagaattgca 6780tgtgtcctca ttgttgtgtt cctattgctg gtggtgctca tacctgagcc agaaaagcaa 6840agatctcccc aggacaacca aatggcaatc atcatcatgg tagcagtagg tcttctgggc 6900ttgattaccg ccaatgaact cggatggttg gagagaacaa agagtgacct aagccatcta 6960atgggaagga gagaggaggg ggcaaccatg ggattctcaa tggacattga cctgcggcca 7020gcctcagctt gggccatcta tgctgccttg acaactttca ttaccccagc cgtccaacat 7080gcagtgacca cttcatacaa caactactcc ttaatggcga tggccacgca agctggagtg 7140ttgtttggta tgggcaaagg gatgccattc tacgcatggg actttggagt cccgctgcta 7200atgataggtt gctactcaca attaacgccc ctgaccctaa tagtggccat cattttgctc 7260gtggcgcact acatgtactt gatcccaggg ctgcaggcag cagctgcgcg tgctgcccag 7320aagagaacgg cagctggcat catgaagaac cctgttgtgg atggaatagt ggtgactgac 7380attgacacaa tgacaattga cccccaagtg gagaaaaaga tgggacaggt gctactcatg 7440gcagtagccg tctccagcgc catactgtcg cggaccgcct gggggtgggg ggaggctggg 7500gccctgatca cagccgcaac ttccactttg tgggaaggct ctccgaacaa gtactggaac 7560tcctctacag ccacttcact gtgtaacatt tttaggggaa gttacttggc tggagcttct 7620ctaatctaca cagtaacaag aaacgctggc ttggtcaaga gacgtggggg tggaacagga 7680gagaccctgg gagagaaatg gaaggcccgc ttgaaccaga tgtcggccct ggagttctac 7740tcctacaaaa agtcaggcat caccgaggtg tgcagagaag aggcccgccg cgccctcaag 7800gacggtgtgg caacgggagg ccatgctgtg tcccgaggaa gtgcaaagct gagatggttg 7860gtggagcggg gatacctgca gccctatgga aaggtcattg atcttggatg tggcagaggg 7920ggctggagtt actacgccgc caccatccgc aaagttcaag aagtgaaagg atacacaaaa 7980ggaggccctg gtcatgaaga acccgtgttg gtgcaaagct atgggtggaa catagtccgt 8040cttaagagtg gggtggacgt ctttcatatg gcggctgagc cgtgtgacac gttgctgtgt 8100gacataggtg agtcatcatc tagtcctgaa gtggaagaag cacggacgct cagagtcctc 8160tccatggtgg gggattggct tgaaaaaaga ccaggagcct tttgtataaa agtgttgtgc 8220ccatacacca gcactatgat ggaaaccctg gagcgactgc agcgtaggta tgggggagga 8280ctggtcagag tgccactctc ccgcaactct acacatgaga tgtactgggt ctctggagcg 8340aaaagcaaca ccataaaaag tgtgtccacc acgagccagc tcctcttggg gcgcatggac 8400gggcctagga ggccagtgaa atatgaggag gatgtgaatc tcggctctgg cacgcgggct 8460gtggtaagct gcgctgaagc tcccaacatg aagatcattg gtaaccgcat tgaaaggatc 8520cgcagtgagc acgcggaaac gtggttcttt gacgagaacc acccatatag gacatgggct 8580taccatggaa gctatgaggc ccccacacaa gggtcagcgt cctctctaat aaacggggtt 8640gtcaggctcc tgtcaaaacc ctgggatgtg gtgactggag tcacaggaat agccatgacc 8700gacaccacac cgtatggtca gcaaagagtt ttcaaggaaa aagtggacac tagggtgcca 8760gacccccaag aaggcactcg tcaggttatg agcatggtct cttcctggtt gtggaaagag 8820ctaggcaaac acaaacggcc acgagtctgt accaaagaag agttcatcaa caaggttcgt 8880agcaatgcag cattaggggc aatatttgaa gaggaaaaag agtggaagac tgcagtggaa 8940gctgtgaacg atccaaggtt ctgggctcta gtggacaagg aaagagagca ccacctgaga 9000ggagagtgcc agagttgtgt gtacaacatg atgggaaaaa gagaaaagaa acaaggggaa 9060tttggaaagg ccaagggcag ccgcgccatc tggtatatgt ggctaggggc tagatttcta 9120gagttcgaag cccttggatt cttgaacgag gatcactgga tggggagaga gaactcagga 9180ggtggtgttg aagggctggg attacaaaga ctcggatatg tcctagaaga gatgagtcgc 9240ataccaggag gaaggatgta tgcagatgac actgctggct gggacacccg catcagcagg 9300tttgatctgg agaatgaagc tctaatcacc aaccaaatgg agaaagggca cagggccttg 9360gcattggcca taatcaagta cacataccaa aacaaagtgg taaaggtcct tagaccagct 9420gaaaaaggga agacagttat ggacattatt tcgagacaag accaaagggg gagcggacaa 9480gttgtcactt acgctcttaa cacatttacc aacctagtgg tgcaactcat tcggaatatg 9540gaggctgagg aagttctaga gatgcaagac ttgtggctgc tgcggaggtc agagaaagtg 9600accaactggt tgcagagcaa cggatgggat aggctcaaac gaatggcagt cagtggagat 9660gattgcgttg tgaagccaat tgatgatagg tttgcacatg ccctcaggtt cttgaatgat 9720atgggaaaag ttaggaagga cacacaagag tggaaaccct caactggatg ggacaactgg 9780gaagaagttc cgttttgctc ccaccacttc aacaagctcc atctcaagga cgggaggtcc 9840attgtggttc cctgccgcca ccaagatgaa ctgattggcc gggcccgcgt ctctccaggg 9900gcgggatgga gcatccggga gactgcttgc ctagcaaaat catatgcgca aatgtggcag 9960ctcctttatt tccacagaag ggacctccga ctgatggcca atgccatttg ttcatctgtg 10020ccagttgact gggttccaac tgggagaact acctggtcaa tccatggaaa gggagaatgg 10080atgaccactg aagacatgct tgtggtgtgg aacagagtgt ggattgagga gaacgaccac 10140atggaagaca agaccccagt tacgaaatgg acagacattc cctatttggg aaaaagggaa 10200gacttgtggt gtggatctct catagggcac agaccgcgca ccacctgggc tgagaacatt 10260aaaaacacag tcaacatggt gcgcaggatc ataggtgatg aagaaaagta catggactac 10320ctatccaccc aagttcgcta cttgggtgaa gaagggtcta cacctggagt gctgtaagca 10380ccaatcttaa tgttgtcagg cctgctagtc agccacagct tggggaaagc tgtgcagcct 10440gtgacccccc caggagaagc tgggaaacca agcctatagt caggccgaga acgccatggc 10500acggaagaag ccatgctgcc tgtgagcccc tcagaggaca ctgagtcaaa aaaccccacg 10560cgcttggagg cgcaggatgg gaaaagaagg tggcgacctt ccccaccctt caatctgggg 10620cctgaactgg agatcagctg tggatctcca gaagagggac tagtggttag aggaga 10676610808DNAZika virus 6agttgttgat ctgtgtgaat cagactgcga cagttcgagt ttgaagcgaa agctagcaac 60agtatcaaca ggttttattt tggatttgga aacgagagtt tctggtcatg aaaaacccaa 120aaaagaaatc cggaggattc cggattgtca atatgctaaa acgcggagta gcccgtgtga 180gcccctttgg gggcttgaag aggctgccag ccggacttct gctgggtcat gggcccatca 240ggatggtctt ggcaattcta gcctttttga gattcacggc aatcaagcca tcactgggtc 300tcatcaatag atggggttca gtggggaaaa aagaggctat ggaaataata aagaagttca 360agaaagatct ggctgccatg ctgagaataa tcaatgctag gaaggagaag aagagacgag 420gcgcagatac tagtgtcgga attgttggcc tcctgctgac cacagctatg gcagcggagg 480tcactagacg tgggagtgca tactatatgt acttggacag aaacgatgct ggggaggcca 540tatcttttcc aaccacattg gggatgaata agtgttatat acagatcatg gatcttggac 600acatgtgtga tgccaccatg agctatgaat gccctatgct ggatgagggg gtggaaccag 660atgacgtcga ttgttggtgc aacacgacgt caacttgggt tgtgtacgga acctgccatc 720acaaaaaagg tgaagcacgg agatctagaa gagctgtgac gctcccctcc cattccacta 780ggaagctgca aacgcggtcg caaacctggt tggaatcaag agaatacaca aagcacttga 840ttagagtcga aaattggata ttcaggaacc ctggcttcgc gttagcagca gctgccatcg 900cttggctttt gggaagctca acgagccaaa aagtcatata cttggtcatg atactgctga 960ttgccccggc atacagcatc aggtgcatag gagtcagcaa tagggacttt gtggaaggta 1020tgtcaggtgg gacttgggtt gatgttgtct tggaacatgg aggttgtgtc accgtaatgg 1080cacaggacaa accgactgtc gacatagagc tggttacaac aacagtcagc aacatggcgg 1140aggtaagatc ctactgctat gaggcatcaa tatcagacat ggcttcggac agccgctgcc 1200caacacaagg tgaagcctac cttgacaagc aatcagacac tcaatatgtc tgcaaaagaa 1260cgttagtgga cagaggctgg ggaaatggat gtggactttt tggcaaaggg agcctggtga 1320catgcgctaa gtttgcatgc tccaagaaaa tgaccgggaa gagcatccag ccagagaatc 1380tggagtaccg gataatgctg tcagttcatg gctcccagca cagtgggatg atcgttaatg 1440acacaggaca tgaaactgat gagaatagag cgaaggttga gataacgccc aattcaccaa 1500gagccgaagc caccctgggg ggttttggaa gcctaggact tgattgtgaa ccgaggacag 1560gccttgactt ttcagatttg tattacttga ctatgaataa caagcactgg ttggtccaca 1620aggagtggtt ccacgacatt ccattacctt ggcacgctgg ggcagacacc ggaactccac 1680actggaacaa caaagaagca ctggtagagt tcaaggacgc acatgccaaa aggcaaactg 1740tcgtggttct agggagtcaa gaaggagcag ttcacacggc ccttgctgga gctctggagg 1800ctgagatgga tggtgcaaag ggaaggctgt cctctggcca cttgaaatgt cgcctgaaaa 1860tggataaact tagattgaag ggcgtgtcat actccttgtg taccgcagcg ttcacattca 1920ccaagatccc ggctgaaaca ctgcacggga cagtcacagt ggaggtacag tacgcaggga 1980cagatggacc ttgcaaggtt

ccagctcaga tggcggtgga catgcaaact ctgaccccag 2040ttgggaggtt gataaccgct aaccccgtaa tcactgaaag cactgagaac tctaagatga 2100tgctggaact tgatccacca tttggggact cttacattgt cataggagtc ggggagaaga 2160agatcaccca ccactggcac aggagtggca gcaccattgg aaaagcattt gaagccactg 2220tgagaggtgc caagagaatg gcagtcttgg gagacacagc ctgggacttt ggatcagttg 2280gaggcgctct caactcattg ggcaagggca tccatcaaat ttttggagca gctttcaaat 2340cattgtttgg aggaatgtcc tggttctcac aaatcctcat tggaacgttg ctgatgtggt 2400tgggtctgaa cacaaagaat ggatctattt cccttatgtg cttggcctta gggggagtgt 2460tgatcttctt atccacagcc gtctctgctg atgtggggtg ctcggtggac ttctcaaaga 2520aggagacgag atgcggtaca ggggtgttcg tctataacga cgttgaagcc tggagggaca 2580ggtacaagta ccatcctgac tccccccgta gattggcagc agcagtcaag caagcctggg 2640aagatggtat ctgcgggatc tcctctgttt caagaatgga gaacatcatg tggagatcag 2700tagaagggga gctcaacgca atcttggaag agaatggagt tcaactgacg gtcgttgtgg 2760gatctgtaaa aaaccccatg tggagaggtc cacagagatt gcccgtgcct gtgaacgagc 2820tgccccacgg ctggaaggct tgggggaaat cgtacttcgt cagagcagca aagacaaata 2880acagctttgt cgtggatggt gacacactga aggaatgccc actcgaacat agagcatgga 2940acagctttct tgtggaggat catgggttcg gggtatttca cactagtgtc tggctcaagg 3000ttagagaaga ttattcatta gagtgtgatc cagccgttat tggaacagct gttaagggga 3060aggaggctgt acacagtgat ctaggctact ggattgagag tgagaagaat gacacatgga 3120ggctgaagag ggcccatcta atcgagatga aaacatgtga atggccaaag tcccacacat 3180tgtgggcaga tggaatagaa gagagtgatc tgatcattcc caagtcttta gctgggccac 3240tcagccatca caataccaga gagggctaca ggacccaaat gaaagggcca tggcacagtg 3300aagagcttga aattcggttt gaggaatgcc cgggcactaa ggtccacgtg gaggaaacat 3360gtggaacaag aggaccatct ctgagatcaa ccactgcaag cggaagggtg atcgaggaat 3420ggtgctgcag ggagtgcaca atgcccccac tgtcgttccg ggctaaagat ggctgttggt 3480atggaatgga gataaggccc aggaaagaac cagaaagcaa cttagtaagg tcagtggtga 3540ctgcaggatc aactgatcac atggatcact tctcccttgg agtgcttgtg attctgctca 3600tggtgcagga agggctgaag aagagaatga ccacaaagat catcataagc acatcaatgg 3660cagtgctggt agctatgatc ctgggaggat tttcaatgag tgacctggct aagcttgcaa 3720ttttgatggg cgccaccttc gcggaaatga acactggagg agatgtagct catctggcgc 3780tgatagcggc attcaaagtc agaccagcgt tgctggtatc tttcatcttc agagctaatt 3840ggacaccccg tgaaagcatg ctgctggcct tggcctcgtg tcttttgcaa actgcgatct 3900ccgccttgga aggcgacctg atggttctca tcaatggttt tgctttggcc tggttggcaa 3960tacgagcgat ggttgttcca cgcactgata acatcacctt ggcaatcctg gctgctctga 4020caccactggc ccggggcaca ctgcttgtgg cgtggagagc aggccttgct acttgcgggg 4080ggtttatgct cctctctctg aagggaaaag gcagtgtgaa gaagaactta ccatttgtca 4140tggccctggg actaaccgct gtgaggctgg tcgaccccat caacgtggtg ggactgctgt 4200tgctcacaag gagtgggaag cggagctggc cccctagcga agtactcaca gctgttggcc 4260tgatatgcgc attggctgga gggttcgcca aggcagatat agagatggct gggcccatgg 4320ccgcggtcgg tctgctaatt gtcagttacg tggtctcagg aaagagtgtg gacatgtaca 4380ttgaaagagc aggtgacatc acatgggaaa aagatgcgga agtcactgga aacagtcccc 4440ggctcgatgt ggcgctagat gagagtggtg atttctccct ggtggaggat gacggtcccc 4500ccatgagaga gatcatactc aaggtggtcc tgatgaccat ctgtggcatg aacccaatag 4560ccataccctt tgcagctgga gcgtggtacg tatacgtgaa gactggaaaa aggagtggtg 4620ctctatggga tgtgcctgct cccaaggaag taaaaaaggg ggagaccaca gatggagtgt 4680acagagtaat gactcgtaga ctgctaggtt caacacaagt tggagtggga gttatgcaag 4740agggggtctt tcacactatg tggcacgtca caaaaggatc cgcgctgaga agcggtgaag 4800ggagacttga tccatactgg ggagatgtca agcaggatct ggtgtcatac tgtggtccat 4860ggaagctaga tgccgcctgg gacgggcaca gcgaggtgca gctcttggcc gtgccccccg 4920gagagagagc gaggaacatc cagactctgc ccggaatatt taagacaaag gatggggaca 4980ttggagcggt tgcgctggat tacccagcag gaacttcagg atctccaatc ctagacaagt 5040gtgggagagt gataggactt tatggcaatg gggtcgtgat caaaaatggg agttatgtta 5100gtgccatcac ccaagggagg agggaggaag agactcctgt tgagtgcttc gagccttcga 5160tgctgaagaa gaagcagcta actgtcttag acttgcatcc tggagctggg aaaaccagga 5220gagttcttcc tgaaatagtc cgtgaagcca taaaaacaag actccgtact gtgatcttag 5280ctccaaccag ggttgtcgct gctgaaatgg aggaagccct tagagggctt ccagtgcgtt 5340atatgacaac agcagtcaat gtcacccact ctggaacaga aatcgtcgac ttaatgtgcc 5400atgccacctt cacttcacgt ctactacagc caatcagagt ccccaactat aatctgtata 5460ttatggatga ggcccacttc acagatccct caagtatagc agcaagagga tacatttcaa 5520caagggttga gatgggcgag gcggctgcca tcttcatgac cgccacgcca ccaggaaccc 5580gtgacgcatt tccggactcc aactcaccaa ttatggacac cgaagtggaa gtcccagaga 5640gagcctggag ctcaggcttt gattgggtga cggatcattc tggaaaaaca gtttggtttg 5700ttccaagcgt gaggaacggc aatgagatcg cagcttgtct gacaaaggct ggaaaacggg 5760tcatacagct cagcagaaag acttttgaga cagagttcca gaaaacaaaa catcaagagt 5820gggactttgt cgtgacaact gacatttcag agatgggcgc caactttaaa gctgaccgtg 5880tcatagattc caggagatgc ctaaagccgg tcatacttga tggcgagaga gtcattttgg 5940ctggacccat gcctgtcaca catgccagcg ctgcccagag gagggggcgc ataggcagga 6000atcccaacaa acctggagat gagtatctgt atggaggtgg gtgcgcagag actgacgaag 6060accatgcaca ctggcttgaa gcaagaatgc tccttgacaa tatttacctc caagatggcc 6120tcatagcctc gctctatcga cctgaggccg acaaagtagc agccattgag ggagagttca 6180agcttaggac ggagcaaagg aagacctttg tggaactcat gaaaagagga gatcttcctg 6240tttggctggc ctatcaggtt gcatctgccg gaataaccta cacagataga agatggtgct 6300ttgatggcac gaccaacaac accataatgg aagacagtgt gccggcagag gtgtggacca 6360gacacggaga gaaaagagtg ctcaaaccga ggtggatgga cgccagagtt tgttcagatc 6420atgcggccct gaagtcattc aaggagtttg ccgctgggaa aagaggagcg gcttttggag 6480tgatggaagc cctgggaaca ctgccaggac acatgacaga gagattccag gaagccattg 6540acaacctcgc tgtgctcatg cgggcagaga ctggaagcag gccttacaaa gccgcggcgg 6600cccaattgcc ggagacccta gagaccatta tgcttttggg gttgctggga acagtctcgc 6660tgggaatctt tttcgtcttg atgaggaaca agggcatagg gaagatgggc tttggaatgg 6720tgactcttgg ggccagcgca tggctcatgt ggctctcgga aattgagcca gccagaattg 6780catgtgtcct cattgttgtg ttcctattgc tggtggtgct catacctgag ccagaaaagc 6840aaagatctcc ccaggacaac caaatggcaa tcatcatcat ggtagcagta ggtcttctgg 6900gcttgattac cgccaatgaa ctcggatggt tggagagaac aaagagtgac ctaagccatc 6960taatgggaag gagagaggag ggagcaacca taggattctc aatggacatt gacctgcggc 7020cagcctcagc ttgggccatc tatgctgcct tgacaacttt cattacccca gccgtccaac 7080atgcagtgac cacttcatac aacaactact ccttaatggc gatggccacg caagctggag 7140tgttgtttgg tatgggcaaa gggatgccat tctacgcatg ggactttgga gtcccgctgc 7200taatgatagg ttgctactca caattaacac ccctgaccct aatagtggcc atcattttgc 7260tcgtggcgca ctacatgtac ttgatcccag ggctgcaggc agcagctgcg cgtgctgccc 7320agaagagaac ggcagctggc atcatgaaga accctgttgt ggatggaata gtggtgactg 7380acattgacac aatgacaatt gacccccaag tggagaaaaa gatgggacag gtgctactca 7440tagcagtagc agtctccagc gccatactgt cgcggaccgc ctgggggtgg ggggaggctg 7500gggccctgat cacagccgca acttccactt tgtgggaagg ctctccgaac aagtactgga 7560actcctctac agccacttca ctgtgtaaca tttttagggg aagttacttg gctggagctt 7620ctctaatcta catagtaaca agaaacgctg gcttggtcaa gagacgtggg ggtggaacag 7680gagagaccct gggagagaaa tggaaggccc gcttgaacca gatgtcggcc ctggagttct 7740actcctacaa aaagtcaggc atcaccgagg tgtgcagaga agaggcccgc cgcgccctca 7800aggatggtgt ggcaacggga ggccatgctg tgtcccgagg aagtgcaaag ctgagatggt 7860tggtggagcg gggatacctg cagccctatg gaaaggtcat tgatcttgga tgtggcagag 7920ggggctggag ttactacgcc gccaccatcc gcaaagttca agaagtgaaa ggatacacaa 7980aaggaggccc tggtcatgaa gaacccgtgt tggtgcaaag ctatgggtgg aacatagtcc 8040gtcttaagag tggggtggac gtctttcata tggcggctga gccgtgtgac acgttgctgt 8100gtgacatagg tgagtcatca tctagtcctg aagtggaaga agcacggacg ctcagagtcc 8160tctccatggt gggggattgg cttgaaaaaa gaccaggagc cttttgtata aaagtgttgt 8220gcccatacac cagcactatg atggaaaccc tggagcgact gcagcgtagg tatgggggag 8280gactggtcag agtgccactc tcccgcaact ctacacatga gatgtactgg gtctctggag 8340cgaaaagcaa caccataaaa agtgtgtcca ccacgagcca gctcctcttg gggcgcatgg 8400acgggcctag gaggccagtg aaatatgagg aggatgtgaa tctcggctct ggcacgcggg 8460ctgtggtaag ctgcgctgaa gctcccaaca tgaagatcat tggtaaccgc attgaaagga 8520tccgcagtga gcacgcggaa acgtggttct ttgacgagaa ccacccatat aggacatggg 8580cttaccatgg aagctatgag gcccccacac aagggtcagc gtcctctcta ataaacgggg 8640ttgtcaggct cctgtcaaaa ccctgggatg tggtgactgg agtcacagga atagccatga 8700ccgacaccac accgtatggt cagcaaagag ttttcaagga aaaagtggac actagggtgc 8760cagaccccca agaaggcact cgtcaggtta tgagcatggt ctcttcctgg ttgtggaaag 8820agctaggcaa acacaaacgg ccacgagtct gtaccaaaga agagttcatc aacaaggttc 8880gtagcaatgc agcattaggg gcaatatttg aagaggaaaa agagtggaag actgcagtgg 8940aagctgtgaa cgatccaagg ttctgggctc tagtggacaa ggaaagagag caccacctga 9000gaggagagtg ccagagttgt gtgtacaaca tgatgggaaa aagagaaaag aaacaagggg 9060aatttggaaa ggccaagggc agccgcgcca tctggtatat gtggctaggg gctagatttc 9120tagagttcga agcccttgga ttcttgaacg aggatcactg gatggggaga gagaactcag 9180gaggtggtgt tgaagggctg ggattacaaa gactcggata tgtcctagaa gagatgagtc 9240gcataccagg aggaaggatg tatgcagatg acactgctgg ctgggacacc cgcatcagca 9300ggttcgatct ggagaatgaa gctctaatca ccaaccaaat ggagaaaggg catagggcct 9360tggcattggc cataatcaag tacacatacc aaaacaaagt ggtaaaggtc cttagaccag 9420ctgaaaaagg gaaaacagtt atggacatta tttcgagaca agaccaaagg gggagcggac 9480aagttgtcac ttacgctctt aacacattta ccaacctagt ggtgcaactc attcggaata 9540tggaggctga ggaagttcta gagatgcaag acttgtggct gctgcggagg tcagagaaag 9600tgaccaactg gttgcagagc aacggatggg ataggctcaa acgaatggca gtcagtggag 9660atgattgcgt tgtgaagcca attgatgata ggtttgcaca tgccctcagg ttcttgaatg 9720atatgggaaa agttaggaag gacacacaag agtggaaacc ctcaactgga tgggacaact 9780gggaagaagt tccgttttgc tcccaccact tcaacaagct ccatctcaag gacgggaggt 9840ccattgtggt tccctgccgc caccaagatg aactgattgg ccgggcccgc gtctctccag 9900gggcgggatg gagcatccgg gagactgctt gcctagcaaa atcatatgcg caaatgtggc 9960agctccttta tttccacaga agggacctcc gactgatggc caatgccatt tgttcatctg 10020tgccagttga ctgggttcca actgggagaa ctacctggtc aatccatgga aagggagaat 10080ggatgaccac tgaagacatg cttgtggtgt ggaacagagt gtggattgag gagaacgacc 10140acatggaaga caagacccca gttacgaaat ggacagacat tccctatttg ggaaaaaggg 10200aagacttgtg gtgtggatct ctcatagggc acagaccgcg caccacctgg gctgagaaca 10260ttaaaaacac agtcaacatg gtgcgcagga tcataggtga tgaagaaaag tacatggact 10320acctatccac ccaagttcgc tacttgggtg aagaagggtc tacacctgga gtgctgtaag 10380caccaatctt aatgttgtca ggcctgctag tcagccacag cttggggaaa gctgtgcagc 10440ctgtgacccc cccaggagaa gctgggaaac caagcctata gtcaggccga gaacgccatg 10500gcacggaaga agccatgctg cctgtgagcc cctcagagga cactgagtca aaaaacccca 10560tgcgcttgga ggcgcaggat gggaaaagaa ggtggcgacc ttccccaccc ttcaatctgg 10620ggcctgaact ggagatcagc tgtggatctc cagaagaggg actagtggtt agaggagacc 10680ccccggaaaa cgcaaaacag catattgacg ctgggaaaga ccagagactc catgagtttc 10740caccacgctg gccgccaggc acagatcgcc gaatagcggc ggccggtgtg gggaaatcca 10800tgggtctt 10808710807DNAZika virus 7agttgttgat ctgtgtgaat cagactgcga cagttcgagt ttgaagcgaa agctagcaac 60agtatcaaca ggttttattt tggatttgga aacgagagtt tctggtcatg aaaaacccaa 120aaaagaaatc cggaggattc cggattgtca atatgctaaa acgcggagta gcccgtgtga 180gcccctttgg gggcttgaag aggctgccag ccggacttct gctgggtcat gggcccatca 240ggatggtctt ggcgattcta gcctttttga gattcacggc aatcaagcca tcactgggtc 300tcatcaatag atggggttca gtgggaaaaa aagaggctat ggaaataata aagaagttca 360agaaagatct ggctgccatg ctgagaataa tcaatgctag gaaggagaag aagagacgag 420gcacagatac tagtgtcgga attgttggcc tcctgctgac cacagctatg gcagcggagg 480tcactagacg tgggagtgca tactatatgt acttggacag aagcgatgct ggggaggcca 540tatcttttcc aaccacactg gggatgaata agtgttatat acagatcatg gatcttggac 600acatgtgtga tgccaccatg agctatgaat gccctatgct ggatgagggg gtagaaccag 660atgacgtcga ttgttggtgc aacacgacgt caacttgggt tgtgtacgga acctgccatc 720acaaaaaagg tgaagcacgg agatccagaa gagctgtgac gctcccctcc cattccacta 780ggaagctgca aacgcggtcg cagacctggt tggaatcaag agaatacaca aagcacttga 840ttagagtcga aaattggata ttcaggaacc ctggcttcgc gttagcagca gctgccatcg 900cttggctttt gggaagctca acgagccaaa aagtcatata cttggtcatg atactgctga 960ttgccccggc atacagcatc aggtgcatag gagtcagtaa tagggacttt gtggaaggta 1020tgtcaggtgg gacttgggtt gatgttgtct tggaacatgg aggttgtgtc accgtaatgg 1080cacaggacaa accgactgtc gacatagagc tggttacaac aacagtcagc aacatggcgg 1140aggtaagatc ctactgctat gaggcatcaa tatcggacat ggcttcggac agccgctgcc 1200caacacaagg tgaagcctac cttgacaagc aatcagacac tcaatatgtc tgcaaaagaa 1260cgttagtgga cagaggctgg ggaaatggat gtggactttt tggcaaaggg agcctggtga 1320catgcgctaa gtttgcatgc tccaagaaaa tgaccgggaa gagcatccag ccagagaatc 1380tggagtaccg gataatgctg tcagttcatg gctcccagca cagtgggatg atcgttaatg 1440acacaggaca tgaaactgat gagaatagag cgaaggttga gataacgccc aattcaccaa 1500gagccgaagc caccctgggg ggttttggaa gcctaggact tgattgtgaa ccgaggacag 1560gccttgactt ttcagatttg tattacttga ctatgaacaa caagcactgg ttggttcaca 1620aggagtggtt ccacgacatt ccattacctt ggcacactgg ggcagacacc ggaactccac 1680actggaacaa caaagaagca ctggtagagt tcaaggacgc acatgccaaa aggcaaactg 1740tcgtggttct agggagtcaa gaaggagcag ttcacacggc ccttgctgga gctctggagg 1800ctgagatgga tggtgcaaag ggaaggctgt cctctggcca cttgaaatgt cgcctgaaaa 1860tggataaact tagattgaag ggcgtgtcat actccttgtg taccgcagcg ttcacattca 1920ccaagatccc ggctgaaaca ctgcacggga cagtcacagt ggaggtacag tacgcaggga 1980cagatggacc ttgcaaggtt ccagctcaga tggcggtgga catgcaaact ctgaccccag 2040ttgggaggtt gataaccgct aaccccgtaa tcactgaagg cactgagaac tctaagatga 2100tgctggaact tgatccacca tttggggact cttacattgt cataggagtc ggggagaaga 2160agatcaccca ccactggcac aggagtggca gcaccattgg aaaagcattt gaagccactg 2220tgagaggtgc caagagaatg gcagtcttgg gagacacagc ctgggacttt ggatcagttg 2280gaggcgttct taactcattg ggcaagggca tccatcaaat ttttggagca gctttcaaat 2340cattgtttgg aggaatgtcc tggttctcac aaattctcat tggaacgttg ctgatgtggt 2400tgggtctgaa tacaaagaat ggatctattt cccttatgtg cttggcctta gggggagtgt 2460tgatcttctt atccacagcc gtctccgctg atgtggggtg ctcggtggac ttctcaaaga 2520aggaaacgag atgcggtaca ggggtgttcg tctataacga cgttgaagcc tggagggaca 2580ggtacaagta ccatcctgac tcccctcgta gattggcagc agtagtcaag caagcctggg 2640aagatggtat ctgtgggatc tcctctgttt caagaatgga aaacatcatg tggagatcag 2700tagaagggga gctcaacgca atcctggaag agaatggagt tcaactgacg gtcgttgtgg 2760gatctgtaaa aaaccccatg tggagaggtc cacagagatt gcccgtgcct gtgaacgagc 2820tgccccacgg ctggaaggct tgggggaaat cgtacttcgt cagagcagca aagacaaata 2880acagctttgt cgtggatggt gacacactga aggaatgccc actcaaacat agagcatgga 2940acagctttct tgtggaggat catgggttcg gggtatttca cactagtgtc tggctcaagg 3000ttagagaaga ttattcacta gagtgtgatc cagccgtcat tggaacagct gttaagggaa 3060aggaggctgt acacagtgat ctaggctact ggattgagag tgagaagaac gacacatgga 3120ggctgaggag ggcccacctg atcgagatga aaacatgtga atggccaaag tcccacacat 3180tgtggacaga tggaatagaa gagagtgatc tgatcatacc caagtcttta gctgggccac 3240tcagccatca caacaccaga gagggctaca ggacccaaat gaaagggcca tggcacagtg 3300aagagcttga aattcggttt gaggaatgcc caggcactaa ggtccacgtg gaggaaacat 3360gtggaacaag aggaccatct ctgagatcaa ccactgcaag cggaagggtg atcgaggaat 3420ggtgctgcag ggagtgcaca atgcccccac tgtcgttccg ggctaaagat ggctgttggt 3480atggaatgga gataaggccc aggaaagaac cagaaagtaa cttagtaagg tcaatggtga 3540ctgcaggatc aactgatcac atggatcact tttcccttgg agtgcttgtg attctgctca 3600tggtgcagga agggctgaag aagagaatga ccacaaagat catcataagc acatcaatgg 3660cagtgctggt agctatgatc ctgggaggat tttcaatgag tgatctggct aagcttgcaa 3720ttttgatggg tgccaccttt gcggaaatga acactggagg agatgtagct catctggcgc 3780tggtagcggc attcaaagtc agaccagcgt tgctggtatc tttcatcttc agagctaatt 3840ggacaccccg tgaaagcatg ctgctggcct tggcctcgtg tcttttgcaa actgcgatct 3900ccgccttgga aggcgacctg atggttctca tcaatggttt tgctttggcc tggttggcaa 3960tacgagcgat ggttgttcca cgcactgaca atatcacctt ggcaatcctg gctgctctga 4020caccactggc ccggggcaca ctgcttgtgg cgtggagagc aggccttgct acttgcgggg 4080ggttcatgct cctctctctg aaggggaaag gcagtgtgaa gaagaactta ccatttgtca 4140tggccctggg actaaccgct gtgaggctgg tcgaccccat caacgtggtg ggactgctgt 4200tgctcacaag gagtgggaag cggagctggc cccctagcga agtactcaca gctgttggcc 4260tgatatgcgc attggctgga gggttcgcca aggcagatat agagatggct gggcccatgg 4320ccgcggtcgg tctgctaatt gtcagttacg tggtctcagg aaagagtgtg gacatgtaca 4380ttgaaagagc aggtgacatc acatgggaaa aagatgcgga agttactgga aacagtcccc 4440ggctcgatgt ggcactagat gagagtggtg atttctccct ggtggaggat gacggtcccc 4500ccatgagaga gatcatactc aaagtggtcc tgatgaccat ctgtggcatg aacccaatag 4560ccataccctt tgcagctgga gcgtggtacg tatacgtgaa aactggaaaa aggagtggtg 4620ctctatggga tgtgcctgct cccaaggaag taaaaaaggg ggagaccaca gatggagtgt 4680acagagtaat gactcgtaga ctgctaggtt caacacaagt tggagtggga gttatgcaag 4740agggggtctt tcacactatg tggcatgtca caaaaggatc cgcgctgaga agcggtgaag 4800ggagacttga tccatactgg ggagatgtca agcaggatct ggtgtcatac tgtggtccat 4860ggaagctaga tgccgcctgg gacgggcaca gcgaggtgca gctcttggcc gtgccccccg 4920gagagagagc gaggaacatc cagactctgc ccggaatatt taagacaaag gatggggaca 4980ttggagcggt tgcgctggac tatccagcag gaacttcagg atctccaatc ctagacaagt 5040gtgggagagt gataggactc tatggcaatg gggtcgtgat caagaatggg agttatgtca 5100gtgccatcac ccaagggagg agggaggaag agactcctgt tgagtgcttc gagccttcga 5160tgctgaagaa gaagcagcta actgtcttag acttgcatcc tggagctggg aaaaccagga 5220gagttcttcc tgaaatagtc cgtgaagcca taaaaacgag actccgtact gtgatcttag 5280ctccaaccag ggttgtcgct gctgaaatgg aggaagccct tagagggctt ccagtgcgtt 5340atatgacaac agcagtcaat gtcacccatt ctgggacaga aatcgttgac ttaatgtgcc 5400atgccacctt cacttcacgt ctactacagc caatcagagt ccccaactat aatctgtata 5460ttatggatga ggcccacttc acagatccct caagtatagc agcaagagga tacatttcaa 5520caagggttga gatgggcgag gcagctgcca tcttcatgac cgccacgcca ccaggaaccc 5580gtgacgcatt cccggactcc aactcaccaa ttatggacac cgaagtggaa gtcccagaga 5640gagcctggag ctcaggcttt gattgggtga cggatcattc tggaaaaaca gtttggtttg 5700tcccaagcgt gaggaacggc aatgagatcg cagcttgtct gacaaaggct ggaaaacggg 5760tcatacagct cagcagaaag acttttgaga cagagttcca gaaaacaaaa catcaagagt 5820gggacttcgt cgtgacaact gacatttcag agatgggcgc caactttaaa gctgaccgtg 5880tcatagattc caggagatgc ctaaagccgg tcatacttga tggcgagaga gtcattctgg 5940ctggacccat gcctgtcaca catgccagcg ctgcccagag gagggggcgc ataggcagga 6000atcccaacaa acctggagat gagtatctgt atggaggtgg gtgcgcagag actgatgaag 6060accatgcaca ctggcttgaa gcaagaatgc tccttgacaa tatttacctc caagatggcc 6120tcatagcctc gctctatcga cctgaggccg acaaagtagc agccattgag ggagagttca

6180agcttaggac ggagcaaagg aagacctttg tggaactcat gaaaagagga gatcttcctg 6240tttggctggc ctatcaggtt gcatctgccg gaataaccta cacagataga agatggtgct 6300ttgatggcat gaccaacaac accataatgg aagacagtgt gccggcagag gtgtggacca 6360gacacggaga gaaaagagtg ctcaaaccga ggtggatgga cgccagagtt tgttcagatc 6420atgcggccct gaagtcattc aaggagtttg ccgctgggaa aagaggagcg gcttttggag 6480tgatggaagc cctgggaaca ctgccaggac acatgacgga gagattccag gaagccattg 6540acaacctcgc tgtgctcatg cgggcagaga ctggaagcag gccttacaaa gccgcggcgg 6600cccaattgcc ggagacccta gagaccatta tgcttttggg gttgctggga acagtctcgc 6660tgggaatctt tttcgtcttg atgcggaaca agggcatagg gaagatgggc tttggaatgg 6720tgactcttgg ggccagcgca tggctcatgt ggctctcgga aattgagcca gccagaattg 6780catgcgtcct cattgttgtg ttcctattgc tggtggtgct catacctgag ccagaaaagc 6840aaagatcccc ccaggacaac caaatggcaa tcatcatcat ggtagcagta ggtcttctgg 6900gcttgattac cgccaatgaa ctcggatggt tggagagaac aaagagtgac ctaagccatc 6960taatgggaag gagagaggag ggggcaacca taggattctc aatggacatt gacctgcggc 7020cagcctcggc ctgggccatc tatgctgccc tgacaacttt cattacccca gccgtccaac 7080atgcagtgac cacttcatac aacaactact ccttaatggc gatggccacg caagctggag 7140tgttgtttgg tatgggcaaa gggatgccat tctacgcatg ggactttgga gtcccgctgc 7200taatgatagg ttgctactca caattaacac ccctgaccct aatagtggct atcattttgc 7260tcgtggcgca ctacatgtac ttgatcccag ggctgcaggc agcagctgcg cgtgctgccc 7320agaagagaac ggcagctggc atcatgaaga accctgttgt ggatggaata gtggtgactg 7380acattgacac aatgactatt gacccccaag tggagaaaaa gatgggacag gtgctactca 7440tagcagtagc cgtctccagc gccatactgt cgcggaccgc ctgggggtgg ggggaagctg 7500gggccctgat cacagctgca acttccactt tgtgggaagg ctctccgaac aagtactgga 7560actcctctac agccacttca ctgtgcaaca tttttagggg aagttacttg gctggagctt 7620ctctaatcta cacagtaaca agaaacgctg gcttggtcaa gagacgtggg ggtggaacag 7680gagagaccct gggagagaaa tggaaggccc gcttgaacca gatgtcggcc ctggagttct 7740actcctacaa aaagtcaggc atcaccgagg tgtgcagaga agaggcccgc cgcgccctca 7800aggacggtgt ggcaacggga ggccatgctg tgtcccgagg aagtgcaaag ctgagatggt 7860tggtggagcg gggatacctg cagccctatg gaaaggtcat tgatcttgga tgtggcagag 7920ggggctggag ttactacgcc gccaccatcc gcaaagttca agaagtgaaa ggatacacaa 7980aaggaggccc tggtcatgaa gaacccatgt tggtgcaaag ctatgggtgg aacatagtcc 8040gtcttaagag tggggtggac gtctttcata tggcggctga gccgtgtgac acgttgctgt 8100gtgacatagg tgagtcatca tctagtcctg aagtggaaga agcacggacg ctcagagtcc 8160tctccatggt gggggattgg cttgaaaaaa gaccaggagc cttttgtgta aaagtgttgt 8220gcccatacac cagcactatg atggaaaccc tggagcgact gcagcgtagg tatgggggag 8280gactggtcag agtgccactc tcccgcaact ctacacatga gatgtactgg gtctctggag 8340cgaaaagcaa caccataaaa agtgtgtcca ccacgagcca gctcctcttg gggcgcatgg 8400acgggcccag gaggccagtg aaatatgagg aggatgtgaa tctcggctct ggcacgcggg 8460ctgtggtaag ctgcgctgaa gctcccaaca tgaagatcat tggtaaccgc attgaaagga 8520tccgcagtga gcacgcggaa acgtggttct ttgacgagaa ccacccatat aggacatggg 8580cttaccatgg aagctatgag gcccctacac aagggtcagc gtcctctcta ataaacgggg 8640ttgtcaggct cctgtcaaaa ccctgggatg tggtgactgg agtcacagga atagccatga 8700ccgacaccac accgtatggt cagcaaagag ttttcaagga aaaagtggac accagggtgc 8760cagaccccca agaaggcact cgtcaggtta tgagcatggt ctcttcctgg ttgtggaaag 8820agctaggcaa acacaaacgg ccacgagtct gtaccaaaga agagttcatc aacaaggttc 8880gtagcaatgc agcattaggg gcaatatttg aagaggaaaa agagtggaag accgcagtgg 8940aagctgtgaa cgatccaagg ttctgggctc tagtggacaa ggaaagagag caccacctga 9000gaggagagtg ccagagctgt gtgtacaaca tgatgggaaa aagagaaaag aaacaagggg 9060aatttggaaa ggccaagggc agccgcgcca tctggtatat gtggctaggg gctagatttc 9120tagagttcga agcccttgga ttcttaaatg aggatcactg gatggggaga gagaactcag 9180gaggtggtgt tgaagggctg ggattacaaa gactcggata tgtcctagaa gagatgagtc 9240gcataccagg aggaaggatg tatgcagatg acactgctgg ctgggacacc cgcatcagca 9300ggtttgatct ggagaatgaa gctttaatca ccaaccaaat ggagaaaggg cacagggcct 9360tagcattggc cataatcaag tacacatacc aaaacaaagt ggtaaaggtc cttagaccag 9420ctgaaaaagg gaagacagtt atggacatta tttcaagaca agaccaaagg gggagcggac 9480aagttgtcac ttacgctctt aacacattta ccaacctagt ggtgcaactc attcggaata 9540tggaggctga ggaagttcta gagatgcaag acttgtggct gctgcggagg tcagagaaag 9600tgaccaactg gttgcagagc aacggatggg ataggctcaa acgaatggca gtcagtggag 9660atgattgcgt tgtgaagcca attgatgata ggtttgcaca tgccctcagg ttcttgaatg 9720atatgggaaa agttaggaag gacacacaag agtggaaacc ctcaactgga tgggacaact 9780gggaagaagt tccgttttgt tcccaccact tcaacaagct ccatctcaag gacgggaggt 9840ccattgtggt tccctgccgc caccaagatg aactgattgg ccgggcccgt gtctctccag 9900gggcgggatg gagcatccgg gagactgctt gcctagcaaa gtcatatgcg caaatgtggc 9960agctccttta tttccacaga agggacctcc gactgatggc caatgccatc tgttcatctg 10020tgccagttga ctgggttcca actgggagaa ctacctggtc aatccatgga aagggagaat 10080ggatgaccac tgaagacatg cttgtggtgt ggaacagagt gtggattgag gagaacgacc 10140acatggaaga caagacccca gttacgaaat ggacagacat tccctatctg ggaaaaaggg 10200aagacttgtg gtgtggatct ctcatagggc acagaccgcg caccacctgg gctgagaaca 10260ttaaaaacac agtcaacatg gtgcgcagga tcataggtga tgaagaaaag tacatggact 10320acctatccac ccaagttcgc tacttgggtg aagaagggtc tacacctgga gtgctataag 10380caccaatctt agtgttgtca ggcctgctag tcagccacag cttggggaaa gctgtgcagc 10440ctgtgacccc cccaggagag gctgggaaac caagcccata gtcaggccga gaacgccatg 10500gcacggaaga agccatgctg cctgtgagcc cctcagagga cactgagtca aaaaacccca 10560cgcgcttgga ggcgcaggat gggaaaagaa ggtggcgacc ttccccaccc ttcaatctgg 10620ggcctgaact ggagatcagc tgtggatctc cagaagaggg actagtggtt agaggagacc 10680ccccggaaaa cgcaaaacag catattgacg ctgggaaaga ccagagactc catgagtttc 10740caccacgctg gccgccaggc acagatcgcc gaatagcggc ggccggtgtg gggaaatcca 10800tgggtct 10807810807DNAZika virus 8agttgttgat ctgtgtgaat cagactgcga cagttcgagt ttgaagcgaa agctagcaac 60agtatcaaca ggttttattt tggatttgga aacgagagtt tctggtcatg aaaaacccaa 120aaaagaaatc cggaggattc cggattgtca atatgctaaa acgcggagta gcccgtgtga 180gcccctttgg gggcttgaag aggctgccag ccggacttct gctgggccat gggcccatca 240ggatggtctt ggcgatacta gcctttttga gattcacggc aatcaagcca tcactgggtc 300tcatcaatag atggggttca gtggggaaaa aagaggctat ggaaataata aagaagttca 360agaaagatct ggctgccatg ctgagaataa tcaatgctag gaaggagaag aagagacgag 420gcgcagatac tagcgtcgga attgttggcc tcctcctgac cacagccatg gcagtagagg 480tcactagacg tgggagtgca tactatatgt acttggacag aagcgatgct ggggaggcca 540tatcttttcc aaccacactg gggatgaata agtgttacat acaaatcatg gatcttggac 600acatgtgtga tgccaccatg agctatgaat gccctatgtt ggatgagggg gtagaaccag 660atgacgtcga ttgctggtgc aacacgacat caacttgggt tgtgtatgga acctgccacc 720acaaaaaagg tgaagcacgg agatctagaa gagctgtgac gctcccctcc cattccacta 780ggaagctgca aacgcggtcg cagacctggt tggaatcaag agaatacaca aagcacctga 840ttagagttga aaattggata ttcaggaacc ctggcttcgc gttagcagca gctgtcatcg 900cttggctttt gggaagttca acgagccaaa aagtcatata tctggtcatg atactgctga 960ttgccccggc atacagcatc aggtgcatag gagtcagcaa tagggacttt gtggaaggta 1020tgtcaggtgg gacttgggtt gatgttgtct tggaacatgg aggttgtgtt accgtaatgg 1080cacaggacaa accgactgtc gacatagagc tggttacaac aacagtcagc aacatggcgg 1140aggtaagatc ctactgctat gaggcatcaa tatcggatat ggcttcggac agccgctgcc 1200caacacaagg tgaggcctac cttgacaagc agtcagacac tcaatatgtc tgcaaaagaa 1260cgttagtgga cagaggctgg ggaaatggat gtggactttt tggcaaaggg agcctggtga 1320catgcgctaa gtttgcatgc tccaagaaaa tgaccgggaa gagcatccag ccagagaatc 1380tggagtaccg gataatgctg tcagttcatg gctcccagca cagtgggatg atcgttaatg 1440acacaggaca tgaaactgat gagaatagag cgaaggttga gataacgccc aattcaccaa 1500gagccgaagc caccctgggg ggttttggga gcctaggact tgattgtgaa ccgaggacag 1560gccttgactt ttcagatttg tattacctga ctatgaataa caagcactgg ttggttcaca 1620aggagtggtt ccacgacatt ccattacctt ggcatgctgg ggcagacact ggaactccac 1680attggaacaa caaagaagca ctggtagagt tcaaggacgc acatgcaaaa aggcaaactg 1740tcgtggttct agggagtcaa gaaggagcag ttcacacggc ccttgctgga gctctggagg 1800ctgagatgga tggagccaag ggaaggctgt cctctggcca cttgaaatgt cgcctgaaaa 1860tggataaact tagattgaag ggcgtgtcat actccttgtg cactgcagcg ttcacattca 1920ccaagatccc ggctgaaaca ctgcacggga cagtcacagt ggaggtacag tacgcaggga 1980cagatggacc ttgcaaggtt ccagctcaga tggcggtgga tatgcaaact ctgaccccag 2040ttgggaggtt gataaccgct aaccctgtaa tcactgaaag caccgagaac tctaagatga 2100tgctggaact tgatccacca tttggggact cttacattgt cataggagtc ggggagaaga 2160agatcaccca tcactggcac aggagtggca gcaccattgg aaaagcattt gaagccactg 2220tgagaggtgc caagagaatg gcagtcttgg gagacacagc ctgggacttt ggatcagttg 2280ggggtgctct caactcattg ggcaagggca tccatcaaat ttttggagca gctttcaaat 2340cattgttcgg aggaatgtcc tggttctcac aaattctcat tggaacgttg ctggtgtggt 2400tgggtctgaa tacaaagaat ggatctattt cccttacgtg cttggcctta gggggagtgt 2460tgatcttctt atccacagcc gtttctgctg atgtggggtg ctcggtggac ttctcaaaga 2520aggaaacgag atgcggtaca ggggtgttcg tctataacga cgttgaagcc tggagggaca 2580ggtacaagta ccatcctgac tcccctcgta gattggcagc agcagtcaag caagcctggg 2640aagatgggat ctgtgggatc tcctctgtct caagaatgga aaacatcatg tggagatcag 2700tagaagggga gctcaacgca atcctggaag agaatggagt tcaactgacg gtcgttgtgg 2760gatctgtaaa aaaccccatg tggagaggtc cacagagatt gcccgtgcct gtgaacgagc 2820tgccccacgg ctggaaggct tgggggaaat cgtacttcgt cagagcagca aagacaaata 2880acagctttgt cgtggatggt gacacactga aggaatgccc actcaaacat agagcatgga 2940acagctttct tgtggaggat catgggtttg gggtatttca cactagtgtc tggctcaagg 3000ttagagaaga ttattcatta gagtgtgatc cagccgtcat tggaacagct gctaagggaa 3060aggaggctgt gcacagcgat ctaggctact ggattgagag tgagaagaac gacacatgga 3120ggctgaagag ggcccacctg atcgagatga aaacatgtga atggccaaag tcccacacat 3180tgtggacaga tggagtagaa gaaagtgatc tgatcatacc caagtcttta gctgggccac 3240tcagccatca caacaccaga gagggctaca ggactcaaat gaaagggcca tggcacagtg 3300aagagcttga aattcggttt gaggaatgcc caggcactaa ggtccacgtg gaggaaacat 3360gtgggacaag aggaccatcc ctgagatcaa ccactgcaag cggaagggtg atcgaggaat 3420ggtgctgcag ggaatgcaca atgcccccac tgtcgttccg agctaaagat ggctgttggt 3480atggaatgga gataaggccc aggaaagaac cagaaagtaa cttagtaagg tcaatggtga 3540ctgcaggatc aactgatcac atggatcact tctctcttgg agtgcttgtg attttgctca 3600tggtgcagga agggctgaag aagagaatga ccacaaagat catcataagc acatcaatgg 3660cagtgctggt agccatgatc ctgggaggat tttcaatgag tgacctggct aagcttgcaa 3720ttttgatggg tgccaccttc gcggaaatga acactggagg agatgtagct catttggcgc 3780tgatagcggc attcaaagtc agacctgcgt tgctggtatc tttcatcttc agagctaatt 3840ggacaccccg tgagagcatg ctgctggcct tggcctcgtg tcttctgcaa actgcgatct 3900ccgccttgga aggcgacctg atggttctca tcaatggttt tgctttggcc tggttggcaa 3960tacgagcgat ggttgttcca cgcactgaca acatcacctt ggcaatcctg gctgctctga 4020caccactggc ccggggcaca ctgcttgtgg cgtggagagc aggccttgct acttgcgggg 4080ggttcatgct cctctctctg aaggggaaag gcagtgtgaa gaagaaccta ccatttgtca 4140tggccttggg actaactgct gtgaggctgg tcgaccccat caacgtggtg ggactgctgt 4200tgctcacaag gagtgggaag cggagctggc cccctagtga agtactcaca gctgttggcc 4260tgatatgcgc attggctgga gggttcgcca aggcggatat agagatggct gggcccatgg 4320ccgcggtcgg tctgctaatt gtcagttacg tggtctcagg aaagagtgtg gacatgtaca 4380ttgaaagagc aggtgacatc acatgggaaa aagatgcgga aatcactgga aacagtcccc 4440ggctcgatgt ggcactagat gagagtggtg atttctccct agtggaggat gatggtccac 4500ccatgagaga gatcatactc aaagtggtcc tgatgaccat ctgcggcatg aacccaatag 4560ccataccctt tgcagctgga gcgtggtacg tgtatgtgaa gactggaaaa aggagtggtg 4620ctctatggga tgtgcctgct cccaaggaag taaaaaaggg ggagaccaca gatggagtgt 4680acagagtaat gactcgtaga ctgcttggtt caacacaagt tggagtggga gtcatgcaag 4740agggggtctt ccacactatg tggcacgtca caaaaggatc cgcgctgaga agcggtgaag 4800ggagacttga tccatactgg ggagatgtca agcaggatct ggtgtcatac tgtggtccgt 4860ggaagctaga cgccgcctgg gacgggcaca gcgaggtgca gctcttggcc gtgccccccg 4920gagagagagc gaggaacatc cagactctgc ccggaacatt taagacaaag gatggggaca 4980ttggagcagt tgcgctggac tacccagcag gaacttcagg atctccaatc ctagacaagt 5040gtgggagagt gataggactc tatggtaatg gggtcgtgat aaaaaatggg agttatgtta 5100gtgccatcac ccaagggagg agggaggaag agactcctgt tgagtgcttc gagccttcga 5160tgctgaagaa gaagcagcta actgtcttag acctgcatcc tggagccggg aaaaccagga 5220gagttcttcc tgaaatagtc cgtgaagcca taaaaacaag actccgtact gtgatcttag 5280ctccaaccag ggtcgtcgct gctgaaatgg aggaagccct tagagggctt ccagttcgtt 5340atatgacaac agcagtcaat gtcacccatt ctgggacaga aatcgttgac ttaatgtgcc 5400atgctacctt cacttcacgc ctactacaac caatcagagt ccccaactat aatttgtata 5460ttatggatga ggcccacttc acagatccct caagtatagc agcaagagga tacatttcaa 5520caagggttga gatgggcgag gcggctgcca tcttcatgac cgccacgcca ccaggaaccc 5580gtgacgcatt cccggactcc aactcaccaa ttatggacac cgaggtggaa gtcccagaga 5640gagcctggag cacaggcttt gattgggtga cggatcattc tgggaaaaca gtctggtttg 5700ttccaagcgt gaggaacggc aatgagatcg cagcttgtct gacaaaggct ggaaaacggg 5760tcatacagct cagcagaaag acttttgaga cagagttcca gaaaacgaaa aatcaagagt 5820gggacttcgt cgtgacaacc gacatttcag agatgggcgc caactttaaa gctgaccgtg 5880tcatagattc caggagatgc ttaaagccgg tcatacttga tggcgagaga gtcattttgg 5940ctggacccat gcctgtcaca catgccagcg ctgctcagag gagggggcgc ataggcagga 6000atcccaacaa acctggagat gagtatctgt atggaggtgg gtgcgcagag actgatgaag 6060atcacgcaca ctggcttgaa gcaagaatgc ttcttgacaa catttacctc caagatggcc 6120tcatagcttc gctctatcga cctgaggccg acaaagtagc agctattgag ggagagttca 6180agcttaggac ggagcaaagg aagacctttg tggaactcat gaaaagagga gatcttccgg 6240tttggttggc ctatcaggtt gcatctgccg gaataaccta cacagataga agatggtgct 6300ttgatggcat gaccaacaac accataatgg aagacagtgt gccggcagag gtgtggacca 6360gatacggaga gaaaagagtg ctcaaaccga ggtggatgga cgccagagtt tgttcagatc 6420atgcggccct gaagtcattc aaagagtttg ccgctgggaa aagaggagcg gcctttggag 6480tgatagaagc cctgggaaca ctgccaggac acatgacaga gagattccag gaagccattg 6540acaacctcgc tgtgctcatg cgggcagaga ctggaagcag gccttacaaa gccgcggcgg 6600cccaattgcc ggagacccta gagaccatta tgcttttggg gttgctggga acagtctcgc 6660tgggaatctt tttcgtcttg atgcggaaca agggcatggg gaagatgggc tttggaatgg 6720tgactcttgg ggccagcgca tggcttatgt ggctctcgga aattgagcca gccagaattg 6780catgtgtcct cattgtcgtg ttcctattgc tggtggtgct catacctgag ccagaaaagc 6840aaagatctcc tcaggacaac caaatggcaa tcatcatcat ggtagcagtg ggtcttctgg 6900gcttgattac cgccaatgaa ctcggatggt tggagagaac aaaaagtgac ctaagccatc 6960taatgggaag gagagaggag ggggcaacca caggattctc aatggacatt gacctgcggc 7020cagcctcagc ttgggctatc tatgctgctc tgacaacttt catcacccca gccgtccaac 7080atgcggtgac cacttcatac aacaactact ccttaatggc gatggccacg caagctgggg 7140tgttgtttgg tatgggcaaa gggatgccat tctacgcatg ggactttgga gtcccgctgc 7200taatgatggg ttgctactca caattaacac ctctgaccct aatagtggcc atcattttgc 7260tcgtggcgca ctacatgtac ttgatcccag ggctgcaggc agcagctgcg cgggctgccc 7320agaagagaac ggcagctggc atcatgaaga accctgttgt ggatggaata gtggtgactg 7380acattgacac aatgacaatt gacccccaag tggaaaaaaa gatggggcag gtgctactca 7440tagcagtagc cgtctccagc gccatactgt cgcggaccgc ctgggggtgg ggggaggctg 7500gggccctgat cacagctgca acttccacct tgtgggaagg ctctccgaac aagtactgga 7560actcctccac agccacttca ctgtgtaaca tttttagggg aagttacttg gctggagctt 7620ctctaatcta cacagtaaca agaaacgctg gcttggtcaa gagacgtggg ggtggaacgg 7680gagagaccct gggagagaaa tggaaggccc gcctgaacca gatgtcggcc ctggagttct 7740actcctacaa aaagtcaggc atcaccgagg tgtgcagaga agaggcccgc cgtgccctca 7800aggacggtgt ggcaacagga ggccatgctg tgtcccgagg aagtgcaaag cttagatggc 7860tggtggagag aggatacctg cagccctatg gaaaggtcat tgatcttgga tgtggcagag 7920ggggctggag ttactatgcc gccaccatcc gcaaagttca ggaagtgaaa ggatacacaa 7980aaggaggccc tggtcatgaa gaacccatgt tggtgcaaag ctatgggtgg aacatagtcc 8040gtcttaagag tggggtggac gtctttcaca tggcggctga gccgtgtgac actttgctgt 8100gtgatatagg tgagtcatca tctagtcctg aagtggaaga agcacggacg ctcagagtcc 8160tctccatggt gggggattgg cttgaaaaaa gaccaggagc cttttgtata aaagtgttgt 8220gcccatacac cagcactatg atggaaaccc tggagcgact gcagcgtagg tatgggggag 8280gactggtcag ggtgccactc tcccgcaact ctacacatga gatgtactgg gtctctggag 8340cgaaaagcaa caccataaaa agtgtgtcca ccacgagcca gctcctcttg gggcgcatgg 8400acgggcccag gaggccagtg aaatatgagg aggatgtgaa tctcggctct ggcacgcggg 8460ctgtggtaag ctgcgctgaa gctcccaaca tgaagatcat tggtaaccgc attgagagga 8520tccgcagtga gcacgcggaa acgtggttct ttgacgagaa ccacccatat aggacatggg 8580cttaccatgg aagctatgag gcccctacac aagggtcagc gtcctctcta ataaacgggg 8640ttgtcaggct cctgtcaaaa ccctgggatg tggtgactgg agtcacagga atagccatga 8700ctgacaccac accgtatggt cagcaaagag ttttcaagga aaaagtggac actagggtgc 8760cagaccccca agaaggcact cgtcaggtta tgagcatggt ctcttcctgg ttatggaagg 8820agctaggcaa acacaaacgg ccacgagtct gtaccaaaga agagttcatc aacaaggttc 8880gtagcaatgc agcattaggg gcaatatttg aagaggaaaa agagtggaag actgcagtgg 8940aagctgtgaa tgatccaagg ttctgggctc tagtggacaa ggaaagagag catcacctga 9000gaggagagtg tcagagctgt gtgtacaaca tgatgggaaa aagagaaaag aaacaagggg 9060aatttggaaa ggccaagggc agccgcgcca tctggtatat gtggctaggg gctagattcc 9120tagagttcga agcccttgga ttcttgaatg aggatcattg gatggggaga gagaattcag 9180gaggtggtgt tgaaggactg ggattacaaa gactcggata tgtcctagaa gagatgagtc 9240gcataccagg aggaaggatg tatgcagatg atactgctgg ctgggacacc cgcatcagca 9300ggtttgatct ggagaatgaa gctctaatca ccaaccaaat ggagaaaggg cacagggcct 9360tggcattggc cataatcaag tacacatacc aaaacaaagt ggtaaaggtc cttagaccag 9420ctgaaaaagg gaagacagtt atggacatta tttcaagaca agaccaaagg gggagcggac 9480aagttgtcac ttacgctctt aatacattca ccaacctggt ggtgcagctc attcggaata 9540tggaggctga ggaagttcta gagatgcaag acttgtggct gctgcggagg ccagagaaag 9600tgaccaactg gttgcaaagc aacggatggg ataggctcaa aagaatggca gtcagtggag 9660atgattgcgt tgtgaaacca attgatgata ggtttgcaca tgccctcagg ttcttgaatg 9720atatgggaaa agttaggaag gacacacaag agtggaaacc ctcaactgga tgggacaact 9780gggaagaagt tccgttttgc tcccaccact tcaacaaact ccatcttaag gacgggaggt 9840ccattgtggt tccctgccgc caccaagatg aactgattgg ccgagcccgc gtatcaccag 9900gggcgggatg gagcatccgg gagactgctt gcctagcaaa atcatatgcg caaatgtggc 9960agctccttta tttccacaga agggacctcc gactgatggc caatgccatt tgttcatctg 10020tgccagttga ttgggttcca actgggagaa ctacctggtc aatccatgga aagggagaat 10080ggatgaccac tgaagacatg cttgtggtat ggaacagagt gtggattgag gaaaacgacc 10140acatggaaga caagacccca gttacaaaat ggacagacat tccctatttg ggaaaaagag 10200aagacttgtg gtgtggatct ctcatagggc acagaccgcg tactacctgg gctgagaaca 10260tcaaaaatac agtcaacatg atgcgcagga tcataggtga tgaagaaaag tacatggact 10320acctatccac ccaggttcgc tacttgggtg aagaagggtc

cacacctgga gtgctgtaag 10380caccaatctt agtgttgtca ggcctgctag tcagccacag cttggggaaa gctgtgcagc 10440ctgtgacccc cccaggagaa gctgggaaac caagcctata gtcaggccga gaacgccatg 10500gcacggaaga agccatgctg cctgtgagcc cctcagagga cactgagtca aaaaacccca 10560cgcgcttgga ggcgcaggat gggaaaagaa ggtggcgacc ttccccaccc ttcaatctgg 10620ggcctgaact ggagatcagc tgtggatctc cagaagaggg actagtggtt agaggagacc 10680ccccggaaaa cgcaaaacag catattgacg ctgggaaaga ccagagactc catgagtttc 10740caccacgctg gccgccaggc acagatcgcc gaatagcggc ggccggtgtg gggaaatcca 10800tgggtct 10807910648DNAZika virus 9gacagttcga gtttgaagcg aaagctagca acagtatcaa caggttttat ttggatttgg 60aaacgagagt ttctggtcat gaaaaaccca aaaaagaaat ccggaggatt ccggattgtc 120aatatgctaa aacgcggagt agcccgtgtg agcccctttg ggggcttgaa gaggctgcca 180gccggacttc tgctgggtca tgggcccatc aggatggtct tggcgattct agcctttttg 240agattcacgg caatcaagcc atcactgggt ctcatcaata gatggggttc agtggggaaa 300aaagaggcta tggaaataat aaagaagttc aagaaagatc tggctgccat gctgagaata 360atcaatgcta ggaaggagaa gaagagacga ggcgcagata ctagtgtcgg aattgttggc 420ctcctgctga ccacagctat ggcagcggag gtcactagac gtgggagtgc atactatatg 480tacttggaca gaaacgatgc tggggaggcc atatcttttc caaccacatt ggggatgaat 540aagtgttata tacagatcat ggatcttgga cacatgtgtg atgccaccat gagctatgaa 600tgccctatgc tggatgaggg ggtggaacca gatgacgtcg attgttggtg caacacgacg 660tcaacttggg ttgtgtacgg aacctgccat cacaaaaaag gtgaagcacg gagatctaga 720agagctgtga cgctcccctc ccattccact aggaagctgc aaacgcggtc gcaaacctgg 780ttggaatcaa gagaatacac aaagcacttg attagagtcg aaaattggat attcaggaac 840cctggcttcg cgttagcagc agctgccatc gcttggcttt tgggaagctc aacgagccaa 900aaagtcatat acttggtcat gatactgctg attgccccgg catacagcat caggtgcata 960ggagtcagca atagggactt tgtggaaggt atgtcaggtg ggacctgggt tgatgttgtc 1020ttggaacatg gaggttgtgt caccgtaatg gcacaggaca aaccgactgt cgacatagag 1080ctggttacaa caacagtcag caacatggcg gaggtaagat cctactgcta tgaggcatca 1140atatcagaca tggcttcgga cagccgctgc ccaacacaag gtgaagccta ccttgacaag 1200caatcagaca ctcaatatgt ctgcaaaaga acgttagtgg acagaggctg gggaaatgga 1260tgtggacttt ttggcaaagg gagcctggtg acatgcgcta agtttgcatg ctccaagaaa 1320atgaccggga agagcatcca gccagagaat ctggagtacc ggataatgct gtcagttcat 1380ggctcccagc acagtgggat gattgttaat gacacaggac atgaaactga tgagaataga 1440gcgaaagttg agataacgcc caattcacca agagccgaag ccaccctggg gggttttgga 1500agcctaggac ttgattgtga accgaggaca ggccttgact tttcagattt gtattacttg 1560actatgaata acaagcactg gttggttcac aaggagtggt tccacgacat tccattacct 1620tggcacgctg gggcagacac cggaactcca cactggaaca acaaagaagc actggtagag 1680ttcaaggacg cacatgccaa aaggcaaact gtcgtggttc tagggagtca agaaggagca 1740gttcacacgg cccttgctgg agctctggag gctgagatgg atggtgcaaa gggaaggctg 1800tcctctggcc acttgaaatg tcgcctgaaa atggataaac ttagattgaa gggcgtgtca 1860tactccttgt gtactgcagc gttcacattc accaagatcc cggctgaaac actgcacggg 1920acagtcacag tggaggtaca gtacgcaggg acagatggac cttgcaaggt tccagctcag 1980atggcggtgg acatgcaaac tctgacccca gttgggaggt tgataaccgc taaccccgta 2040atcactgaaa gcactgagaa ctctaagatg atgctggaac ttgatccacc atttggggac 2100tcttacattg tcataggagt cggggagaag aagatcaccc accactggca caggagtggc 2160agcaccattg gaaaagcatt tgaagccact gtgagaggtg ccaagagaat ggcagtcttg 2220ggagacacag cctgggactt tggatcagtt ggaggcgctc tcaactcatt gggcaagggc 2280atccatcaaa tttttggagc agctttcaaa tcattgtttg gaggaatgtc ctggttctca 2340caaattctca ttggaacgtt gctgatgtgg ttgggtctga acacaaagaa tggatctatt 2400tcccttatgt gcttggcctt agggggagtg ttgatcttct tatccacagc cgtctctgct 2460gatgtggggt gctcggtgga cttctcaaag aaggagacga gatgcggtac aggggtgttc 2520gtctataacg acgttgaagc ctggagggac aggtacaagt accatcctga ctccccccgt 2580agattggcag cagcagtcaa gcaagcctgg gaagatggta tctgcgggat ctcctctgtt 2640tcaagaatgg aaaacatcat gtggagatca gtagaagggg agctcaacgc aatcctggaa 2700gagaatggag ttcaactgac ggtcgttgtg ggatctgtaa aaaaccccat gtggagaggt 2760ccacagagat tgcccgtgcc tgtgaacgag ctgccccacg gctggaaggc ttgggggaaa 2820tcgtacttcg tcagagcagc aaagacaaat aacagctttg tcgtggatgg tgacacactg 2880aaggaatgcc cactcaaaca tagagcatgg aacagctttc ttgtggagga tcatgggttc 2940ggggtatttc acactagtgt ctggctcaag gttagagaag attattcatt agagtgtgat 3000ccagccgtta ttggaacagc tgttaaggga aaggaggctg tacacagtga tctaggctac 3060tggattgaga gtgagaagaa tgacacatgg aggctgaaga gggcccatct gatcgagatg 3120aaaacatgtg aatggccaaa gtcccacaca ttgtggacag atggaataga agagagtgat 3180ctgatcatac ccaagtcttt agctgggcca ctcagccatc acaataccag agagggctac 3240aggacccaaa tgaaagggcc atggcacagt gaagagcttg aaattcggtt tgaggaatgc 3300ccaggcacta aggtccacgt ggaggaaaca tgtggaacaa gaggaccatc tctgagatca 3360accactgcaa gcggaagggt gatcgaggaa tggtgctgca gggagtgcac aatgccccca 3420ctgtcgttcc gggctaaaga tggctgttgg tatggaatgg agataaggcc caggaaagaa 3480ccagaaagca acttagtaag gtcaatggtg actgcaggat caactgatca catggaccac 3540ttctcccttg gagtgcttgt gattctgctc atggtgcagg aagggctgaa gaagagaatg 3600accacaaaga tcatcataag cacatcaatg gcagtgctgg tagctatgat cctgggagga 3660ttttcaatga gtgacctggc taagcttgca attttgatgg gtgccacctt cgcggaaatg 3720aacactggag gagatgtagc tcatctggcg ctgatagcgg cattcaaagt cagaccagcg 3780ttgctggtat ctttcatctt cagagctaat tggacacccc gtgaaagcat gctgctggcc 3840ttggcctcgt gtcttttgca aactgcgatc tccgccttgg aaggcgacct gatggttctc 3900atcaatggtt ttgctttggc ctggttggca atacgagcga tggttgttcc acgcactgat 3960aacatcacct tggcaatcct ggctgctctg acaccactgg cccggggcac actgcttgtg 4020gcgtggagag caggccttgc tacttgcggg gggtttatgc tcctctctct gaagggaaaa 4080ggcagtgtga agaagaactt accatttgtc atggccctgg gactaaccgc tgtgaggctg 4140gtcgacccca tcaacgtggt gggactgctg ttgctcacaa ggagtgggaa gcggagctgg 4200ccccctagcg aagtactcac agctgttggc ctgatatgcg cattggctgg agggttcgcc 4260aaggcagata tagagatggc tgggcccatg gccgcggtcg gtctgctaat tgtcagttac 4320gtggtctcag gaaagagtgt ggacatgtac attgaaagag caggtgacat cacatgggaa 4380aaagatgcgg aagtcactgg aaacagtccc cggctcgatg tggcgctaga tgagagtggt 4440gatttctccc tggtggagga tgacggtccc cccatgagag agatcatact caaggtggtc 4500ctgatgacca tctgtggcat gaacccaata gccataccct ttgcagctgg agcgtggtac 4560gtatacgtga agactggaaa aaggagtggt gctctatggg atgtgcctgc tcccaaggaa 4620gtaaaaaagg gggagaccac agatggagtg tacagagtaa tgactcgtag actgctaggt 4680tcaacacaag ttggagtggg agttatgcaa gagggggtct ttcacactat gtggcacgtc 4740acaaaaggat ccgcgctgag aagcggtgaa gggagacttg atccatactg gggagatgtc 4800aagcaggatc tggtgtcata ctgtggtcca tggaagctag atgccgcctg ggacgggcac 4860agcgaggtgc agctcttggc cgtgcccccc ggagagagag cgaggaacat ccagactctg 4920cccggaatat ttaagacaaa ggatggggac attggagcgg ttgcgctgga ttacccagca 4980ggaacttcag gatctccaat cctagacaag tgtgggagag tgataggact ttatggcaat 5040ggggtcgtga tcaaaaatgg gagttatgtt agtgccatca cccaagggag gagggaggaa 5100gagactcctg ttgagtgctt cgagccttcg atgctgaaga agaagcagct aactgtctta 5160gacttgcatc ctggagctgg gaaaaccagg agagttcttc ctgaaatagt ccgtgaagcc 5220ataaaaacaa gactccgtac tgtgatctta gctccaacca gggttgtcgc tgctgaaatg 5280gaggaggccc ttagagggct tccagtgcgt tatatgacaa cagcagtcaa tgtcacccac 5340tctggaacag aaatcgtcga cttaatgtgc catgccacct tcacttcacg tctactacag 5400ccaatcagag tccccaacta taatctgtat attatggatg aggcccactt cacagatccc 5460tcaagtatag cagcaagagg atacatttca acaagggttg agatgggcga ggcggctgcc 5520atcttcatga ccgccacgcc accaggaacc cgtgacgcat ttccggactc caactcacca 5580attatggaca ccgaagtgga agtcccagag agagcctgga gctcaggctt tgattgggtg 5640acggatcatt ctggaaaaac agtttggttt gttccaagcg tgaggaacgg caatgagatc 5700gcagcttgtc tgacaaaggc tggaaaacgg gtcatacagc tcagcagaaa gacttttgag 5760acagagttcc agaaaacaaa acatcaagag tgggactttg tcgtgacaac tgacatttca 5820gagatgggcg ccaactttaa agctgaccgt gtcatagatt ccaggagatg cctaaagccg 5880gtcatacttg atggcgagag agtcattctg gctggaccca tgcctgtcac acatgccagc 5940gctgcccaga ggagggggcg cataggcagg aatcccaaca aacctggaga tgagtatctg 6000tatggaggtg ggtgcgcaga gactgacgaa gaccatgcac actggcttga agcaagaatg 6060ctccttgaca atatttacct ccaagatggc ctcatagcct cgctctatcg acctgaggcc 6120gacaaagtag cagccattga gggagagttc aagcttagga cggagcaaag gaagaccttt 6180gtggaactca tgaaaagagg agatcttcct gtttggctgg cctatcaggt tgcatctgcc 6240ggaataacct acacagatag aagatggtgc tttgatggca cgaccaacaa caccataatg 6300gaagacagtg tgccggcaga ggtgtggacc agacacggag agaaaagagt gctcaaaccg 6360aggtggatgg acgccagagt ttgttcagat catgcggccc tgaagtcatt caaggagttt 6420gccgctggga aaagaggagc ggcttttgga gtgatggaag ccctgggaac actgccagga 6480cacatgacag agagattcca ggaagccatt gacaacctcg ctgtgctcat gcgggcagag 6540actggaagca ggccttacaa agccgcggcg gcccaattgc cggagaccct agagaccatt 6600atgcttttgg ggttgctggg aacagtctcg ctgggaatct tcttcgtctt gatgaggaac 6660aagggcatag ggaagatggg ctttggaatg gtgactcttg gggccagcgc atggctcatg 6720tggctctcgg aaattgagcc agccagaatt gcatgtgtcc tcattgttgt gtttctattg 6780ctggtggtgc tcatacctga gccagaaaag caaagatctc cccaggacaa ccaaatggca 6840atcatcatca tggtagcagt aggtcttctg ggcttgatta ccgccaatga actcggatgg 6900ttggagagaa caaagagtga cctaagccat ctaatgggaa ggagagagga gggggcaacc 6960ataggattct caatggacat tgacctgcgg ccagcctcag cttgggccat ctatgctgcc 7020ttgacaactt tcattacccc agccgtccaa catgcagtga ccacttcata caacaactac 7080tccttaatgg cgatggccac gcaagctgga gtgttgtttg gtatgggcaa agggatgcca 7140ttctacgcat gggactttgg agtcccgctg ctaatgatag gttgctactc acaattaaca 7200cccctgaccc taatagtggc catcattttg ctcgtggcgc actacatgta cttgatccca 7260gggctgcagg cagcagctgc gcgtgctgcc cagaagagaa cggcagctgg catcatgaag 7320aaccctgttg tggatggaat agtggtgact gacattgaca caatgacaat tgacccccaa 7380gtggagaaaa agatgggaca ggtgctactc atagcagtag ccgtctccag cgccatactg 7440tcgcggaccg cctgggggtg gggggaggct ggggccctga tcacagccgc aacttccact 7500ttgtgggaag gctctccgaa caagtactgg aactcctcta cagccacttc actgtgtaac 7560atttttaggg gaagttactt ggctggagct tctctaatct acacagtaac aagaaacgct 7620ggcttggtca agagacgtgg gggtggaaca ggagagaccc tgggagagaa atggaaggcc 7680cgcttgaacc agatgtcggc cctggagttc tactcctaca aaaagtcagg catcaccgag 7740gtgtgcagag aagaggcccg ccgcgccctc aaggacggtg tggcaacggg aggccatgct 7800gtgtcccgag gaagtgcaaa gctgagatgg ttggtggagc ggggatacct gcagccctat 7860ggaaaggtca ttgatcttgg atgtggcaga gggggctgga gttactacgc cgccaccatc 7920cgcaaagttc aagaagtgaa aggatacaca aaaggaggcc ctggtcatga agaacccgtg 7980ttggtgcaaa gctatgggtg gaacatagtc cgtcttaaga gtggggtgga cgtctttcat 8040atggcggctg agccgtgtga cacgttgctg tgtgacatag gtgagtcatc atctagtcct 8100gaagtggaag aagcacggac gctcagagtc ctctccatgg tgggggattg gcttgaaaaa 8160agaccaggag ccttttgtat aaaggtgttg tgcccataca ccagcactat gatggaaacc 8220ctggagcgac tgcagcgtag gtatggggga ggactggtca gagtgccact ctcccgcaac 8280tctacacatg agatgtattg ggtctctgga gcgaaaagca acaccataaa aagtgtgtcc 8340accacgagcc agctcctctt ggggcgcatg gacgggccta ggaggccagt gaaatatgag 8400gaggatgtga atctcggctc tggcacgcgg gctgtggtaa gctgcgctga agctcccaac 8460atgaagatca ttggtaaccg cattgaaagg atccgcagtg agcacgcgga aacgtggttc 8520tttgacgaga accacccata taggacatgg gcttaccatg gaagctatga ggcccccaca 8580caagggtcag cgtcctctct aataaacggg gttgtcaggc tcctgtcaaa accctgggat 8640gtggtgactg gagtcacagg aatagccatg accgacacca caccgtatgg tcagcaaaga 8700gttttcaagg aaaaagtgga cactagggtg ccagaccccc aagaaggcac tcgtcaggtt 8760atgagcatgg tctcttcctg gttgtggaaa gagctaggca aacacaaacg gccacgagtc 8820tgtaccaaag aagagttcat caacaaggtt cgtagcaatg cagcattagg ggcaatattt 8880gaagaggaaa aagagtggaa gactgcagtg gaagctgtga acgatccaag gttctgggct 8940ctagtggata aggaaagaga gcaccacctg agaggagagt gccagagttg tgtgtacaac 9000atgatgggaa aaagagaaaa gaaacaaggg gaatttggaa aggccaaggg cagccgcgcc 9060atctggtata tgtggctagg ggctagattt ctagagttcg aagcccttgg attcttgaac 9120gaggatcact ggatggggag agagaactca ggaggtggtg ttgaagggct gggattacaa 9180agactcggat atgtcctaga agagatgagt cgtataccag gaggaaggat gtatgcagat 9240gacactgctg gctgggacac ccgcatcagc aggtttgatc tggagaatga agctctaatc 9300accaaccaaa tggaaaaagg gcacagggcc ttggcattgg ccataatcaa gtacacatac 9360caaaacaaag tggtaaaggt ccttagacca gctgaaaaag ggaaaacagt tatggacatt 9420atttcgagac aagaccaaag ggggagcgga caagttgtca cttacgctct taacacattt 9480accaacctag tggtgcaact cattcggaat atggaggctg aggaagttct agagatgcaa 9540gacttgtggc tgctgcggag gtcagagaaa gtgaccaact ggttgcagag caacggatgg 9600gataggctca aacgaatggc agtcagtgga gatgattgcg ttgtgaagcc aattgatgat 9660aggtttgcac atgccctcag gttcttgaat gatatgggaa aagttaggaa ggacacacaa 9720gagtggaaac cctcaactgg atgggacaac tgggaagaag ttccgttttg ctcccaccac 9780ttcaacaagc tccatctcaa ggacgggagg tccattgtgg ttccctgccg ccaccaagat 9840gaactgattg gccgggcccg cgtctctcca ggggcgggat ggagcatccg ggagactgct 9900tgcctagcaa aatcatatgc gcaaatgtgg cagctccttt atttccacag aagggacctc 9960cgactgatgg ccaatgccat ttgttcatct gtgccagttg actgggttcc aactgggaga 10020actacctggt caatccatgg aaagggagaa tggatgacca ctgaagacat gcttgtggtg 10080tggaacagag tgtggattga ggagaacgac cacatggaag acaagacccc agttacgaaa 10140tggacagaca tcccctattt gggaaaaagg gaagacttgt ggtgtggatc tctcataggg 10200cacagaccgc gcaccacctg ggctgagaac attaaaaaca cagtcaacat ggtgcgcagg 10260atcataggtg atgaagaaaa gtacatggac tacctatcca cccaagttcg ctacttgggt 10320gaagaagggt ctacacctgg agtgctgtaa gcaccagtct taatgttgtc aggcctgcta 10380gtcagccaca gcttggggaa agctgtgcag cctgtgaccc ccccaggaga agctgggaaa 10440ccaagcctat agtcaggccg agaacgccat ggcacggaag aagccatgct gcctgtgagc 10500ccctcagagg acactgagtc aaaaaacccc acgcgcttgg aggcgcagga tgggaaaaga 10560aggtggcgac cttccccacc cttcaatctg gggcctgaac tggagatcag ctgtggatct 10620ccagaagagg gactagtggt tagaggag 106481010676DNAZika virus 10gttgttactg ttgctgactc agactgcgac agttcgagtt tgaagcgaaa gctagcaaca 60gtatcaacag gttttatttg gatttggaaa cgagagtttc tggtcatgaa aaacccaaaa 120aagaaatccg gaggattccg gattgtcaat atgctaaaac gcggagtagc ccgtgtgagc 180ccctttgggg gcttgaagag gctgccagcc ggacttctgc tgggtcatgg gcccatcagg 240atggtcttgg caattctagc ctttttgaga ttcacggcaa tcaagccatc actgggtctc 300atcaatagat ggggttcagt ggggaaaaaa gatgctatgg aaataataaa gaagttcaag 360aaagatctgg ctgccatgct gagaataatc aatgctagga aggagaagaa gagacgaggc 420gcagatacta gtgtcggaat tgttggcctc ctgctgacca cagctatggc agcggaggtc 480actagacgtg ggagtgcata ctatatgtac ttggacagaa acgatgctgg ggaggccata 540tcttttccaa ccacattggg gatgaataag tgttatatac agatcatgga tcttggacac 600atgtgtgatg ccaccatgag ctatgaatgc cctatgctgg atgagggggt ggaaccagat 660gacgtcgatt gttggtgcaa cacgacgtca acttgggttg tgtacggaac ctgccatcac 720aaaaaaggtg aagcacggag atctagaaga gctgtgacgc tcccttccca ttccactagg 780aagctgcaaa cgcggtcgca aacctggttg gaatcaagag aatacacaaa gcacttgatt 840agagtcgaaa attggatatt caggaaccct ggcttcgcgt tagcagcagc tgccatcgct 900tggcttttgg gaagctcaac gagccaaaaa gtcatatact tggtcatgat actgctgatt 960gccccggcat acagcatcag gtgcatagga gtcagcaata gggactttgt ggaaggtatg 1020tcaggtggga cttgggttga tgttgtcttg gaacatggag gttgtgtcac cgcaatggca 1080caggacaaac cgactgtcga catagagctg gttacaacaa cagtcagcaa catggcggag 1140gtaagatcct actgctatga ggcatcaata tcagacatgg cttcggacag ccgctgccca 1200acacaaggtg aagcctacct tgacaagcaa tcagacactc aatatgtttg caaaagaacg 1260ttagtggaca gaggctgggg aaatggatgt ggactttttg gcaaagggag tctggtgaca 1320tgcgctaagt ttgcatgctc caagaaaatg accgggaaga gcatccagcc agagaatctg 1380gagtaccgga taatgctgtc agttcatggc tcccagcaca gtgggatgct cgttaatgac 1440acaggacatg aaactgatga gaatagagcg aaggttgaga taacgcccaa ttcaccaaga 1500gccgaagcca ccctgggggg ttttggaagc ctaggacttg attgtgaacc gaggacaggc 1560cttgactttt cagatttgta ttacttgact atgaataaca agcactggtt ggctcacaag 1620gagtggttcc acgacattcc attaccttgg cacgctgggg cagccaccgg aactccacac 1680tggaacaaca aagaagcact ggtagagttc aaggacgcac atgccaaaag gcaaactgtc 1740gtggttctag ggagtcaaga aggagcagtt cacacggccc ttgctggagc tctggaggct 1800gagatggatg gtgcaaaggg aaggctgtcc tctggccact tgaaatgtcg cctgaaaatg 1860gataaactta gattgaaggg cgtgtcatac tccttgtgta ccgcagcgtt cacattcacc 1920aagatcccgg ctgaaacagt ggacgggaca gtcacagtgg agggacagta cggagggaca 1980gatggacctt gcaaggttcc agctcagatg gcggtggaca tgcagactct gaccccagtt 2040gggaggttga taaccgctaa ccccgtaatc actgaaagca ctgagaactc taagatgatg 2100ctggaacttg atccaccatt tggggactct tacattgtca taggagtcgg ggagaagaag 2160atcacccacc actggcacag gagtggcagc accattggaa aagcatttga agccactgtg 2220agaggtgcca agagaatggc agtcttggga gacacagcct gggactttgg atcagttgga 2280ggcgctctca actcattggg caagggcatc catcaaatta ttggagcagc tttcaaatca 2340ttgtttggag gaatgtcctg gttctcacaa attctcattg ggacgttgct gatgtggttg 2400ggtctgaaca caaagaatgg atctatttcc cttatgtgct tggccttagg gggagtgttg 2460atcttcttat ccacagccgt ctcaggtggt gtggggtgct cggtggactt ctcaaagaag 2520gagacgagat gcggtacagg ggtgttcgtc tataacgatg ttgaagcctg gagggacagg 2580tacaagtacc atcctgactc cccccgtaga ttggcagcag cagtcaagca agcctgggaa 2640gatggtatct gcgggatctc ctctgtttca agaatggaaa acatcatgtg gagatcagta 2700gaaggggagc tcaacgcaat cctggaagag aatggagttc aactgacggt cgttgtggga 2760tctgtaaaaa accccatgtg gagaggtcca cagagattgc ccgtgcctgt gaacgagctg 2820ccccacggct ggaaggcttg ggggaaatcg tacttcgtca gagcagcaaa gacaaataac 2880agctttgtcg tggatggtga cacactgaag gaatgcccac tcaaacatag agcatggaac 2940agctttcttg tggaggatca tgggttcggg gtatttcaca ctagtgtctg gctcaaggtt 3000agagaagact attggttaga gtgtgatcca gccgttattg gaacagctgt taagggaaag 3060gaggctgtac acagtgatct aggctactgg attgagagtg agaagaatga cacatggtgg 3120ctgaagaggg cccatctgat cgagatgaaa acatgtgaat ggccaaagtc ccacacattg 3180tggacagatg gaatagaaga gagtgatctg atcataccca agtctttagc tgggccactc 3240agccatcaca atgccagaga gggctacagg acccaaatga aagggccatg gcacagtgaa 3300gagcttgaaa ttcggtttga ggaatgccca ggcactaagg tccacgtgga ggaaacatgt 3360ggaacaagag gaccatctct gagatcaacc actgcaagcg gaagggtgat cgaggaatgg 3420tgctccaggg agtgcacaat gcccccactg tccttccagg ctaaagatgg ctgttggtat 3480ggaatggaga taaggcccag gaaagaacca gaaagcaact tagtaaggtc aatggtgact 3540gcaggatcaa ctgatcacat ggatcacttc tcccttggag tgcttgtgat tctgctcatg 3600gtgcaggaag ggctgaagaa gagaatgacc acaaagatca tcataagcac atcaatggca 3660gtgctggtag ctatgatcct gggaggattt tcaatgagtg acctggctaa gcttgcaatt 3720ttgatgggtg ccaccttcgc ggaaatgaac actggaggag atgtagctca tctggcgctg 3780atagcggcat tcaaagtcag accagcgttg ctggtatctt tcatcttcag agctaattgg

3840acaccccgtg aaagcatgct gctggccttg gcctcgtgtc ttttgcaaac tgcgatctcc 3900gccttggaag gcgacctgat ggttctcatc aatggttttg ctttggcctg gttggcaata 3960cgagcgatgg ttgttccacg cactgataac atcaccttag caatcctggc tgctctgaca 4020ccactggccc ggggcacact gcttgtggcg tggagagcag gccttgctac ttgcgggggg 4080tttatgctcc tctctctgaa gggaaaaggc agtgtgaaga agaacttacc atttgtcatg 4140gccctgggac taaccgctgt gaggctggtc gaccccatca acgtggtggg actgctgttg 4200ctcacaagga gtgggaagcg gagctggccc cctagcgaag tactcacagc tgttggcctg 4260atatgcgcat tggctggagg gttcgccaag gcagatatag agatggctgg gcccatggcc 4320gcggtcggtc tgctaattgt cagttacgtg gtctcaggaa agagtgtgga catgtacatt 4380gaaagagcag gtgacatcac atgggaaaaa gatgcggaag tcactggaaa cagtccccgg 4440ctcgatgtgg cgctagatga gagtggtgat ttctccctgg tggaggatga cggtcccccc 4500atgagagaga tcatactcaa ggtggtcctg atgaccatct gtggcatgaa cccaatagcc 4560ataccctttg cagctggagc gtggtacgta tacgtgaaga ctggaaaaag gagtggtgct 4620ctatgggatg tgcctgctcc caaggaagta aaaaaggggg agaccacaga tggagtgtac 4680agagtaatga ctcgcagact gctaggttca acacaagttg gagtgggagt tatgcaagag 4740ggggtctttc acactatgtg gcacgtcaca aaaggatccg cgctgagaag cggtgaaggg 4800agacttgatc catactgggg agatgtcaag caggatctgg tgtcatactg tggtccatgg 4860aagctagatg ccgcctggga cgggcacagc gaggtgcagc tcttggccgt gccccccgga 4920gagagagcga ggaacatcca gactctgccc ggaatattta agacaaagga tggggacatt 4980ggagcggttg cactggatta cccagcagga acttcaggat ctccaatcct agacaagtgt 5040gggagagtga taggacttta tggcaatggg gtcgtgatca aaaatgggag ttatgttagt 5100gccatcaccc aagggaggag ggaggaagag actcctgttg agtgcttcga gccttcgatg 5160ctgaagaaga agcagctaac tgtcttagac ttgcatcctg gagctgggaa aaccaggaga 5220gttcttcctg aaatagtccg tgaagccata aaaacaagac tccgtactgt gatcttggct 5280ccaaccaggg ttgtcgctgc tgaaatggag gaggccctta gagggcttcc agtgcgttat 5340atgacaacag cagtcaatgt cacccactct ggaacagaaa tcgtcgactt aatgtgccat 5400gccaccttca cttcacgtct actacagcca attagagtcc ccaactataa tctgtatatt 5460atggatgagg cccacttcac agatccctca agtatagcag caagaggata catttcaaca 5520agggttgaga tgggcgaggc ggctgccatc ttcatgaccg ccacgccacc aggaacccgt 5580gacgcatttc cggactccaa ctcaccaatt atggacaccg aagtggaagt cccagagaga 5640gcctggagct caggctttga ttgggtgacg gagtattctg gaaaaacagt ttggtttgtt 5700ccacgcgtga ggaacggcaa tgagatcgca gcttgtctga caaaggctgg aaaacgggtc 5760atacagctca gcagaaagac ttttgagaca gagttccaga aaacaaaaca tcaagagtgg 5820gactttgtcg tgacaactga catttcagag atgggcgcca actttaaagc tgaccgtgtc 5880atagattcca ggagatgcct aaagccggtc atacttggtg gcgagagagt cattctggct 5940ggacccatgc ctgtcacaca tgccagcgct gcccagagga gggggcgcat aggcaggaat 6000cccaacaaac ctggagatga gtatctgtat ggaggtgggt gcgcagagac tgacgaagac 6060catgcacact ggcttgaagc aagaatgctc cttgacaata tttacctcca agatggcctc 6120atagcctcgc tctatcgacc tgaggccgac aaagtagcag ccattgaggg agagttcaag 6180cttaggacgg agcaaaggaa gacctttgtg gaactcatga aaagaggaga tcttcctgtt 6240tggctggcct atcaggttgc atctgccgga ataacctaca cagatagaag atggtgcttt 6300gatggcacga ccaacaacac cataatggaa gacagtgtgc cggcagaggt gtggaccaga 6360cacggagaga aaagagtgct caaaccgagg tggatggacg ccagagtttg ttcagatcat 6420gcggccctga agtcattcaa ggagtttgcc gctgggaaaa gaggagcggc ttttggagtg 6480atggaagccc tgggaacact gccaggacac atgacagaga gattccagga agccattgac 6540aacctcgctg tgctcatgcg ggcagagact ggaagcaggc cttacaaagc cgcggcggcc 6600caattgccgg agaccctaga gaccattatg cttttggggt tgctgggaac agtctcgctg 6660ggaatctttt tcgtcttgat gaggaacaag ggcataggga agatgggctt tggaatggtg 6720actcttgggg ccagcgcatg gctcatgtgg ctctcggaaa ttgagccagc cagaattgca 6780tgtgtcctca ttgttgtgtt cctattgctg gtggtgctca tacctgagcc agaaaagcaa 6840agatctcccc aggacaacca aatggccatc atcatcatgg tagcagtagg tcttctgggc 6900ttgattaccg ccaatgaact cggatggttg gagagaacaa agagtgacct aagccatcta 6960atgggaagga gagaggaggg ggcaaccatg ggattctcaa tggacattga cctgcggcca 7020gcctcagctt gggccatcta tcctgccttg acatctttca ttaccccagc cgtccaacat 7080gcagtgacca cttcatacaa caactactcc ttaatggcga tggccacgca agctggagtg 7140ttgtttggta tgggcaaagg gatgccattc tacgcatggg actttggagt cccgctgcta 7200atgataggtt gctactcaca attaacgccc ctgaccctaa tagtggccat cattttgctc 7260gtggcgcact acatgtactt gatcccaggg ctgcaggcag cagctgcgcg tgctgcccag 7320aagagaacgg cagctggcat catgaagaac cctgttgtgg agggaatagt ggtgactgac 7380attgacacaa tgacaattga cccccaagtg gagaaaaaga tgggacaggt gctactcatg 7440gcagtagccg tctccagcgc catactgtcg aggaccgcct gggggtgggg ggaggctggg 7500gccctgatca cagccgcaac ttccactttg tgggaaggct ctccgaacaa gtactggaac 7560tcctctacag ccacctcact gtgtaacatt tttaggggaa gttacttggc tggagcttct 7620ctaatctaca cagtaacaag aaacgctggc ttggtcaaga gacgtggggg tggaacagga 7680gagaccctgg gagagaaatg gaaggcccgc ttgaaccaga tgtcggccct ggagttctac 7740tcctacaaaa agtcaggcat caccgaggtg tgcagagaag aggcccgccg cgccctcaag 7800gacggtgtgg caacgggagg ccatgctgtg tcccgaggaa gtgcaaagct gagatggttg 7860gtggagcggg gatacctgca gccctatgga aaggtcattg atcttggatg tggcagaggg 7920ggctggagtt actacgccgc caccatccgc aaagttcaag aagtgaaagg atacacaaaa 7980ggaggccctg gtcatgaaga acccgtgttg gtgcaaagct atgggtggaa catagtccgt 8040cttaagagtg gggtggacgt ctttcatatg gcggctgagc cgtgtgacac gttgctgtgt 8100gacataggtg agtcatcatc tagtcctgaa gtggaagaag cacggacgct cagagtcctc 8160tccatggtgg gggattggct tgaaaaaaga ccaggagcct tttgtataaa agtgttgtgc 8220ccatacacca gcactatgat ggaaaccctg gagcgactgc agcgtaggta tgggggagga 8280ctggtcagag tgccactctc ccgcaactct acacatgaga tgtactgggt ctctggagcg 8340aaaagcaaca ccataaaaag tgtgtccacc acgagccagc tcctcttggg gcgcatggac 8400gggcctagga ggccagtgaa atatgaggag gatgtgaatc tcggctctgg cacgcgggct 8460gtggtaagct gcgctgaagc tcccaacatg aagatcattg gtaaccgcat tgaaaggatc 8520cgcgctgaga aagcggaaac gtggttcttt gacgagaacc acccatatag gacatgggct 8580taccatggaa gctatgatgc cgccacacaa gggtcagcgt cctctctaat aaacggggtt 8640gtcaggctcc tgtcaaaacc ctgggatgtg gtgactggag tcacaggaat agccatgacc 8700gacaccacac cgtatggtca gcaaagagtt ttcaaggaaa aagtggacac tagggtgcca 8760gacccccaag aaggcactcg tcaggttatg agcatggtct cttcctggtt gtggaaagag 8820ctaggcaaac acaaacggcc acgagtctgt accaaagaag agttcatcaa caaggttcgt 8880agcaatgcag cattaggggc aatatttgaa gaggaaaaag agtggaagac tgcagtggaa 8940gctgtgaacg atccaaggtt ctgggctcta gtggacaagg aaagagagca ccacctgaga 9000ggagagtgcc agagttgtgt gtacatcaca atgggaaaaa gagaaaagaa acaaggggaa 9060tttggaaagg ccaagggcag ccgcgccatc tggtatatgt ggctaggggc tagatttcta 9120gagttcgaag cccttggatt cttgaacgag gatcactgga tggggagaga gaactcagga 9180ggtggtgttg aagggctggg attacaaaga ctcggatatg tcctagaaga gatgagtcgc 9240ataccaggag gaaggatgta tgcagatgac actgctggct gggacacccg catcagcagg 9300tttgatctgg agaatgaagc tctaatcacc aaccaaatgg agaaagggca cagggccttg 9360gcattggcca taatcaagta cacataccaa aacaaagtgg taaaggtcct tagaccagct 9420gaaaaaggga agacagttat ggacattatt tcgagacaag accaaagggg gagcggacaa 9480gttgtcactt acgctctcaa cacatttacc aacctagtgg tgcaactcat tcggaatatg 9540gaggctgagg aagttctaga gatgcaagac ttgtggctgc tgcggaggtc agagaaagtg 9600accaactggt tgcagagcaa cggatgggat aggctcaaac gaatggcggt cagtggagat 9660gattgcgttg tgaaaccaat tgatgatagg tttgcacatg ccctcaggtt cttgaatgat 9720atgggaaaag ttaggaagga cacacaagag tggaaaccct caactggatg ggacaactgg 9780gaagaagttc ccttctgctc ccaccacttc aacaagctcc atctcaagga cgggaggtcc 9840attgtggttc cctgccgcca ccaagatgaa ctgattggcc gggcccgcgt ctctccaggg 9900gcgggatgga gcatccggga gactgcttgc ctagcaaaat catatgcgca aatgtggcag 9960ctcctttatt tccacagaag ggacctccga ctgatggcca atgccatttg ttcatctgtg 10020ccagttgact gggttccaac tgggagaact acctggtcaa tccatggaaa gggagaatgg 10080atgaccactg aagacatgct tgtggcgtgg aacagagtgt ggattgagga gaacgaccac 10140atggaagaca agaccccagt cacgaaatgg acagacattc cctatttggg aaaaagggaa 10200gacttgtggt gtggatctct catagggcac agaccgcgca ccacctgggc tgagaacatt 10260aaaaacacag tcaacatggt gcgcaggatc ataggtgatg aagaaaagta catggactac 10320ctatccaccc aagttcgcta cttgggtgaa gaagggtcta cacctggagt gctgtaagca 10380ccaatcttaa tgttgtcagg cctgctagtc agccacagct tggggaaagc tgtgcagcct 10440gtgacccccc caggagaagc tgggaaacca agcctatagt caggccgaga acgccatggc 10500acggaagaag ccatgctgcc tgtgagcccc tcagaggaca ctgagtcaaa aaaccccacg 10560cgcttggagg cgcaggatgg gaaaagaagg tggcgacctt ccccaccctt caatctgggg 10620cctgaactgg agatcagctg tggatctcca gaagagggac tagtggttag aggaga 106761110807DNAZika virus 11agttgttgat ctgtgtgagt cagactgcga cagttcgagt ctgaagcgag agctaacaac 60agtatcaaca ggtttaattt ggatttggaa acgagagttt ctggtcatga aaaacccaaa 120gaagaaatcc ggaggattcc ggattgtcaa tatgctaaaa cgcggagtag cccgtgtaaa 180ccccttggga ggtttgaaga ggttgccagc cggacttctg ctgggtcatg gacccatcag 240aatggttttg gcgatactag cctttttgag atttacagca atcaagccat cactgggcct 300tatcaacaga tggggttccg tggggaaaaa agaggctatg gaaataataa agaagttcaa 360gaaagatctt gctgccatgt tgagaataat caatgctagg aaagagagga agagacgtgg 420cgcagacacc agcatcggaa tcattggcct cctgctgact acagccatgg cagcagagat 480cactagacgc gggagtgcat actacatgta cttggatagg agcgatgccg ggaaggccat 540ttcgtttgct accacattgg gagtgaacaa gtgccacgta cagatcatgg acctcgggca 600catgtgtgac gccaccatga gttatgagtg ccctatgctg gatgagggag tggaaccaga 660tgatgtcgat tgctggtgca acacgacatc aacttgggtt gtgtacggaa cctgtcatca 720caaaaaaggt gaggcacggc gatctagaag agccgtgacg ctcccttctc actctacaag 780gaagttgcaa acgcggtcgc agacctggtt agaatcaaga gaatacacga agcacttgat 840caaggttgaa aactggatat tcaggaaccc cgggtttgcg ctagtggccg ttgccattgc 900ctggcttttg ggaagctcga cgagccaaaa agtcatatac ttggtcatga tactgctgat 960tgccccggca tacagtatca ggtgcattgg agtcagcaat agagacttcg tggagggcat 1020gtcaggtggg acctgggttg atgttgtctt ggaacatgga ggctgcgtta ccgtgatggc 1080acaggacaag ccaacagttg acatagagtt ggtcacgacg acggttagta acatggccga 1140ggtaagatcc tattgctacg aggcatcgat atcggacatg gcttcggaca gtcgttgccc 1200aacacaaggt gaagcctacc ttgacaagca atcagacact caatatgtct gcaaaagaac 1260attagtggac agaggttggg gaaacggttg tggacttttt ggcaaaggga gcttggtgac 1320atgtgccaag tttacgtgtt ctaagaagat gaccgggaag agcattcaac cggaaaatct 1380ggagtatcgg ataatgctat cagtgcatgg ctcccagcat agcgggatga ctgtcaatga 1440tataggatat gaaactgacg aaaatagagc gaaagtcgag gttacgccta attcaccaag 1500agcggaagca accttgggag gctttggaag cttaggactt gactgtgaac caaggacagg 1560ccttgacttt tcagatctgt attacctgac catgaacaat aagcattggt tggtgcacaa 1620agagtggttt catgacatcc cattgccttg gcatgctggg gcagacactg gaactccaca 1680ctggaacaac aaagaggcat tggtagaatt caaggatgcc cacgccaaga ggcaaaccgt 1740cgtcgttctg gggagccagg aaggagccgt tcacacggct ctcgctggag ctctagaggc 1800tgagatggat ggtgcaaagg gaaagctgtt ctctggccat ttgaaatgcc gcctaaaaat 1860ggacaagctt agattgaagg gcgtgtcata ttccttgtgc actgcggcat tcacattcac 1920caaggtccca gctgaaacac tgcatggaac agtcacagtg gaggtgcagt atgcagggac 1980agatggaccc tgcaagatcc cagtccagat ggcggtggac atgcagaccc tgaccccagt 2040tggaaggctg ataaccgcca accccgtgat tactgaaagc actgagaact caaagatgat 2100gttggagctt gacccaccat ttggggattc ttacattgtc ataggagttg gggacaagaa 2160aatcacccac cactggcata ggagtggtag caccatcgga aaggcatttg aggccactgt 2220gagaggcgcc aagagaatgg cagtcctggg ggatacagcc tgggacttcg gatcagtcgg 2280gggtgtgttc aactcactgg gtaagggcat tcaccagatt tttggagcag ccttcaaatc 2340actgtttgga ggaatgtcct ggttctcaca gatcctcata ggcacgctgc tagtgtggtt 2400aggtttgaac acaaagaatg gatctatctc cctcacatgc ttggccctgg ggggagtgat 2460gatcttcctc tccacggctg tttctgctga cgtggggtgc tcagtggact tctcaaaaaa 2520ggaaacgaga tgtggcacgg gggtattcat ctataatgat gttgaagcct ggagggaccg 2580gtacaagtac catcctgact ccccccgcag attggcagca gcagtcaagc aggcctggga 2640agaggggatc tgtgggatct catccgtttc aagaatggaa aacatcatgt ggaaatcagt 2700agaaggggag ctcaatgcta tcctagagga gaatggagtt caactgacag ttgttgtggg 2760atctgtaaaa aaccccatgt ggagaggtcc acaaagattg ccagtgcctg tgaatgagct 2820gccccatggc tggaaagcct gggggaaatc gtattttgtt agggcggcaa agaccaacaa 2880cagttttgtt gtcgacggtg acacactgaa ggaatgtccg cttgagcaca gagcatggaa 2940tagttttctt gtggaggatc acgggtttgg agtcttccac accagtgtct ggcttaaggt 3000cagagaagat tactcattag aatgtgaccc agccgtcata ggaacagctg ttaagggaag 3060ggaggccgcg cacagtgatc tgggctattg gattgaaagt gaaaagaatg acacatggag 3120gctgaagagg gcccacctga ttgagatgaa aacatgtgaa tggccaaagt ctcacacatt 3180gtggacagat ggagtagaag aaagtgatct tatcataccc aagtctttag ctggtccact 3240cagccaccac aacaccagag agggttacag aacccaagtg aaagggccat ggcacagtga 3300agagcttgaa atccggtttg aggaatgtcc aggcaccaag gtttacgtgg aggagacatg 3360cggaactaga ggaccatctc tgagatcaac tactgcaagt ggaagggtca ttgaggaatg 3420gtgctgtagg gaatgcacaa tgcccccact atcgtttcga gcaaaagacg gctgctggta 3480tggaatggag ataaggccca ggaaagaacc agagagcaac ttagtgaggt caatggtgac 3540agcggggtca accgatcata tggaccactt ctctcttgga gtgcttgtga ttctactcat 3600ggtgcaggag gggttgaaga agagaatgac cacaaagatc atcatgagca catcaatggc 3660agtgctggta gtcatgatct tgggaggatt ttcaatgagt gacctggcca agcttgtgat 3720cctgatgggt gctactttcg cagaaatgaa cactggagga gatgtagctc acttggcatt 3780ggtagcggca tttaaagtca gaccagcctt gctggtctcc ttcattttca gagccaattg 3840gacaccccgt gagagcatgc tgctagccct ggcttcgtgt cttctgcaaa ctgcgatctc 3900tgctcttgaa ggtgacttga tggtcctcat taatggattt gctttggcct ggttggcaat 3960tcgagcaatg gccgtgccac gcactgacaa catcgctcta ccaatcttgg ctgctctaac 4020accactagct cgaggcacac tgctcgtggc atggagagcg ggcctggcta cttgtggagg 4080gatcatgctc ctctccctga aagggaaagg tagtgtgaag aagaacctgc catttgtcat 4140ggccctggga ttgacagctg tgagggtagt agaccctatt aatgtggtag gactactgtt 4200actcacaagg agtgggaagc ggagctggcc ccctagtgaa gttctcacag ccgttggcct 4260gatatgtgca ctggccggag ggtttgccaa ggcagacatt gagatggctg gacccatggc 4320tgcagtaggc ttgctaattg tcagctatgt ggtctcggga aagagtgtgg acatgtacat 4380tgaaagagca ggtgacatca catgggaaaa ggacgcggaa gtcactggaa acagtcctcg 4440gcttgacgtg gcactggatg agagtggtga tttctccttg gtagaggaag atggtccacc 4500catgagagag atcatactta aggtggtcct gatggccatc tgtggcatga acccaatagc 4560tatacctttt gctgcaggag cgtggtatgt gtatgtgaag actgggaaaa ggagtggcgc 4620cctctgggac gtgcctgctc ccaaagaagt gaagaaagga gagaccacag atggagtgta 4680cagagtgatg actcgcagac tgctaggttc aacacaggtt ggagtgggag tcatgcaaga 4740gggagtcttc cacaccatgt ggcacgttac aaaaggagcc gcactgagga gcggtgaggg 4800aagacttgat ccatactggg gggatgtcaa gcaggacttg gtgtcatact gtgggccttg 4860gaagttggat gcagcttggg atggactcag cgaggtacag cttttggccg tacctcccgg 4920agagagggcc agaaacattc agaccctgcc tggaatattc aagacaaagg acggggacat 4980cggagcagtt gctctggact accctgcagg gacctcagga tctccgatcc tagacaaatg 5040tggaagagtg ataggactct atggcaatgg ggttgtgatc aagaatggaa gctatgttag 5100tgctataacc cagggaaaga gggaggagga gactccggtt gaatgtttcg aaccctcgat 5160gctgaagaag aagcagctaa ctgtcttgga tctgcatcca ggagccggaa aaaccaggag 5220agttcttcct gaaatagtcc gtgaagccat aaaaaagaga ctccggacag tgatcttggc 5280accaactagg gttgtcgctg ctgagatgga ggaggccttg agaggacttc cggtgcgtta 5340catgacaaca gcagtcaacg tcacccattc tgggacagaa atcgttgatt tgatgtgcca 5400tgccactttc acttcacgct tactacaacc catcagagtc cctaattaca atctctacat 5460catggatgaa gcccacttca cagacccctc aagtatagct gcaagaggat atatatcaac 5520aagggttgaa atgggcgagg cggctgccat ttttatgact gccacaccac caggaacccg 5580tgatgcgttt cctgactcta actcaccaat catggacaca gaagtggaag tcccagagag 5640agcctggagc tcaggctttg attgggtgac agaccattct gggaaaacag tttggttcgt 5700tccaagcgtg agaaacggaa atgaaatcgc agcctgtctg acaaaggctg gaaagcgggt 5760catacagctc agcaggaaga cttttgagac agaatttcag aaaacaaaaa atcaagagtg 5820ggactttgtc ataacaactg acatctcaga gatgggcgcc aacttcaagg ctgaccgggt 5880catagactct aggagatgcc taaaaccagt catacttgat ggtgagagag tcatcttggc 5940tgggcccatg cctgtcacgc atgctagtgc tgctcagagg agaggacgta taggcaggaa 6000ccctaacaaa cctggagatg agtacatgta tggaggtggg tgtgcagaga ctgatgaagg 6060ccatgcacac tggcttgaag caagaatgct tcttgacaac atctacctcc aggatggcct 6120catagcctcg ctctatcggc ctgaggccga taaggtagcc gccattgagg gagagtttaa 6180gctgaggaca gagcaaagga agaccttcgt ggaactcatg aagagaggag accttcccgt 6240ctggctagcc tatcaggttg catctgccgg aataacttac acagacagaa gatggtgctt 6300tgatggcaca accaacaaca ccataatgga agacagcgta ccagcagagg tgtggacaaa 6360gtatggagag aagagagtgc tcaaaccgag atggatggat gctagggtct gttcagacca 6420tgcggccctg aagtcgttca aagaattcgc cgctggaaaa agaggagcgg ctttgggagt 6480aatggaggcc ctgggaacac tgccaggaca catgacagag aggtttcagg aagccattga 6540caacctcgcc gtgctcatgc gagcagagac tggaagcagg ccttataagg cagcggcagc 6600ccaactgccg gagaccctag agaccattat gctcttaggt ttgctgggaa cagtttcact 6660ggggatcttc ttcgtcttga tgcggaataa gggcatcggg aagatgggct ttggaatggt 6720aacccttggg gccagtgcat ggctcatgtg gctttcggaa attgaaccag ccagaattgc 6780atgtgtcctc attgttgtgt ttttattact ggtggtgctc atacccgagc cagagaagca 6840aagatctccc caagataacc agatggcaat tatcatcatg gtggcagtgg gccttctagg 6900tttgataact gcaaacgaac ttggatggct ggaaagaaca aaaaatgaca tagctcatct 6960aatgggaagg agagaagaag gagcaaccat gggattctca atggacattg atctgcggcc 7020agcctccgcc tgggctatct atgccgcatt gacaactctc atcaccccag ctgtccaaca 7080tgcggtaacc acttcataca acaactactc cttaatggcg atggccacac aagctggagt 7140gctgtttggc atgggcaaag ggatgccatt ttatgcatgg gaccttggag tcccgctgct 7200aatgatgggt tgctattcac aattaacacc cctgactctg atagtagcta tcattctgct 7260tgtggcgcac tacatgtact tgatcccagg cctacaagcg gcagcagcgc gtgctgccca 7320gaaaaggaca gcagctggca tcatgaagaa tcccgttgtg gatggaatag tggtaactga 7380cattgacaca atgacaatag acccccaggt ggagaagaag atgggacaag tgttactcat 7440agcagtagcc atctccagtg ctgtgctgct gcggaccgcc tggggatggg gggaggctgg 7500agctctgatc acagcagcga cctccacctt gtgggaaggc tctccaaaca aatactggaa 7560ctcctctaca gccacctcac tgtgcaacat cttcagagga agctatctgg caggagcttc 7620ccttatctat acagtgacga gaaacgctgg cctggttaag agacgtggag gtgggacggg 7680agagactctg ggagagaagt ggaaagctcg tctgaatcag atgtcggccc tggagttcta 7740ctcttataaa aagtcaggta tcactgaagt gtgtagagag gaggctcgcc gtgccctcaa 7800ggatggagtg gccacaggag gacatgccgt atcccgggga agtgcaaagc tcagatggtt 7860ggtggagaga ggatatctgc agccctatgg gaaggttgtt gacctcggat gtggcagagg 7920gggctggagc tattatgccg ccaccatccg caaagtgcag gaggtgagag gatacacaaa 7980gggaggtccc ggtcatgaag aacccatgct ggtgcaaagc tatgggtgga acatagttcg 8040tctcaagagt ggagtggacg tcttccacat ggcggctgag ccgtgtgaca ctctgctgtg 8100tgacataggt gagtcatcat ctagtcctga agtggaagag acacgaacac tcagagtgct 8160ctctatggtg ggggactggc ttgaaaaaag accaggggcc

ttctgtataa aggtgctgtg 8220cccatacacc agcactatga tggaaaccat ggagcgactg caacgtaggc atgggggagg 8280attagtcaga gtgccattgt ctcgcaactc cacacatgag atgtactggg tctctggggc 8340aaagagcaac atcataaaaa gtgtgtccac cacaagtcag ctcctcctgg gacgcatgga 8400tggccccagg aggccagtga aatatgagga ggatgtgaac ctcggctcgg gtacacgagc 8460tgtggcaagc tgtgctgagg ctcctaacat gaaaatcatc ggcaggcgca ttgagagaat 8520ccgcaatgaa catgcagaaa catggtttct tgatgaaaac cacccataca ggacatgggc 8580ctaccatggg agctacgaag cccccacgca aggatcagcg tcttccctcg tgaacggggt 8640tgttagactc ctgtcaaagc cttgggacgt ggtgactgga gttacaggaa tagccatgac 8700tgacaccaca ccatacggcc aacaaagagt cttcaaagaa aaagtggaca ccagggtgcc 8760agatccccaa gaaggcactc gccaggtaat gaacatagtc tcttcctggc tgtggaagga 8820gctggggaaa cgcaagcggc cacgcgtctg caccaaagaa gagtttatca acaaggtgcg 8880cagcaatgca gcactgggag caatatttga agaggaaaaa gaatggaaga cggctgtgga 8940agctgtgaat gatccaaggt tttgggccct agtggatagg gagagagaac accacctgag 9000aggagagtgt cacagctgtg tgtacaacat gatgggaaaa agagaaaaga agcaaggaga 9060gttcgggaaa gcaaaaggta gccgcgccat ctggtacatg tggttgggag ccagattctt 9120ggagtttgaa gcccttggat tcttgaacga ggaccattgg atgggaagag aaaactcagg 9180aggtggagtc gaagggttag gattgcaaag acttggatac attctagaag aaatgaatcg 9240ggcaccagga ggaaagatgt acgcagatga cactgctggc tgggacaccc gcattagtaa 9300gtttgatctg gagaatgaag ctctgattac caaccaaatg gaggaagggc acagaactct 9360ggcgttggcc gtgattaaat acacatacca aaacaaagtg gtgaaggttc tcagaccagc 9420tgaaggagga aaaacagtta tggacatcat ttcaagacaa gaccagagag ggagtggaca 9480agttgtcact tatgctctca acacattcac caacttggtg gtgcagctta tccggaacat 9540ggaagctgag gaagtgttag agatgcaaga cttatggttg ttgaggaagc cagagaaagt 9600gaccagatgg ttgcagagca atggatggga tagactcaaa cgaatggcgg tcagtggaga 9660tgactgcgtt gtgaagccaa tcgatgatag gtttgcacat gccctcaggt tcttgaatga 9720catgggaaaa gttaggaaag acacacagga gtggaaaccc tcgactggat ggagcaattg 9780ggaagaagtc ccgttctgct cccaccactt caacaagctg tacctcaagg atgggagatc 9840cattgtggtc ccttgccgcc accaagatga actgattggc cgagctcgcg tctcaccagg 9900ggcaggatgg agcatccggg agactgcctg tcttgcaaaa tcatatgcgc agatgtggca 9960gctcctttat ttccacagaa gagaccttcg actgatggct aatgccattt gctcggctgt 10020gccagttgac tgggtaccaa ctgggagaac cacctggtca atccatggaa agggagaatg 10080gatgaccact gaggacatgc tcatggtgtg gaatagagtg tggattgagg agaacgacca 10140tatggaggac aagactcctg taacaaaatg gacagacatt ccctatctag gaaaaaggga 10200ggacttatgg tgtggatccc ttatagggca cagaccccgc accacttggg ctgaaaacat 10260caaagacaca gtcaacatgg tgcgcaggat cataggtgat gaagaaaagt acatggacta 10320tctatccacc caagtccgct acttgggtga ggaagggtcc acacccggag tgttgtaagc 10380accaatttta gtgttgtcag gcctgctagt cagccacagt ttggggaaag ctgtgcagcc 10440tgtaaccccc ccaggagaag ctgggaaacc aagctcatag tcaggccgag aacgccatgg 10500cacggaagaa gccatgctgc ctgtgagccc ctcagaggac actgagtcaa aaaaccccac 10560gcgcttggaa gcgcaggatg ggaaaagaag gtggcgacct tccccaccct tcaatctggg 10620gcctgaactg gagactagct gtgaatctcc agcagaggga ctagtggtta gaggagaccc 10680cccggaaaac gcaaaacagc atattgacgc tgggaaagac cagagactcc atgagtttcc 10740accacgctgg ccgccaggca cagatcgccg aacagcggcg gccggtgtgg ggaaatccat 10800ggtttct 108071210794DNAZika virus 12agttgttgat ctgtgtgagt cagactgcga cagttcgagt ctgaagcgag agctaacaac 60agtatcaaca ggtttaattt ggatttggaa acgagagttt ctggtcatga aaaaccccaa 120agaagaaatc cggaggatcc ggattgtcaa tatgctaaaa cgcggagtag cccgtgtaaa 180ccccttggga ggtttgaaga ggttgccagc cggacttctg ctgggtcatg gacccatcag 240aatggttttg gcgatactag cctttttgag atttacagca atcaagccat cactgggcct 300tatcaacaga tggggttccg tggggaaaaa agaggctatg gaaataataa agaagttcaa 360gaaagatctt gctgccatgt tgagaataat caatgctagg aaagagagga agagacgtgg 420cgcagacacc agcatcggaa tcattggcct cctgctgact acagccatgg cagcagagat 480cactagacgc gggagtgcat actacatgta cttggatagg agcgatgccg ggaaggccat 540ttcgtttgct accacattgg gagtgaacaa gtgccacgta cagatcatgg acctcgggca 600catgtgtgac gccaccatga gttatgagtg ccctatgctg gatgagggag tggaaccaga 660tgatgtcgat tgctggtgca acacgacatc aacttgggtt gtgtacggaa cctgtcatca 720caaaaaaggt gaggcacggc gatctagaag agccgtgacg ctcccttctc actctacaag 780gaagttgcaa acgcggtcgc agacctggtt agaatcaaga gaatacacga agcacttgat 840caaggttgaa aactggatat tcaggaaccc cgggtttgcg ctagtggccg ttgccattgc 900ctggcttttg ggaagctcga cgagccaaaa agtcatatac ttggtcatga tactgctgat 960tgccccggca tacagtatca ggtgcattgg agtcagcaat agagacttcg tggagggcat 1020gtcaggtggg acctgggttg atgttgtctt ggaacatgga ggctgcgtta ccgtgatggc 1080acaggacaag ccaacagtcg acatagagtt ggtcacgacg acggttagta acatggccga 1140ggtaagatcc tattgctacg aggcatcgat atcggacatg gcttcggaca gtcgttgccc 1200aacacaaggt gaagcctacc ttgacaagca atcagacact caatatgtct gcaaaagaac 1260attagtggac agaggttggg gaaacggttg tggacttttt ggcaaaggga gcttggtgac 1320atgtgccaag tttacgtgtt ctaagaagat gaccgggaag agcattcaac cggaaaatct 1380ggagtatcgg ataatgctat cagtgcatgg ctcccagcat agcgggatga ttggatatga 1440aactgacgaa gatagagcga aagtcgaggt tacgcctaat tcaccaagag cggaagcaac 1500cttgggaggc tttggaagct taggacttga ctgtgaacca aggacaggcc ttgacttttc 1560agatctgtat tacctgacca tgaacaataa gcattggttg gtgcacaaag agtggtttca 1620tgacatccca ttgccttggc atgctggggc agacaccgga actccacact ggaacaacaa 1680agaggcattg gtagaattca aggatgccca cgccaagagg caaaccgtcg tcgttctggg 1740gagccaggaa ggagccgttc acacggctct cgctggagct ctagaggctg agatggatgg 1800tgcaaaggga aggctgttct ctggccattt gaaatgccgc ctaaaaatgg acaagcttag 1860attgaagggc gtgtcatatt ccttgtgcac tgcggcattc acattcacca aggtcccagc 1920tgaaacactg catggaacag tcacagtgga ggtgcagtat gcagggacag atggaccctg 1980caagatccca gtccagatgg cggtggacat gcagaccctg accccagttg gaaggctgat 2040aaccgccaac cccgtgatta ctgaaagcac tgagaactca aagatgatgt tggagcttga 2100cccaccattt ggggattctt acattgtcat aggagttggg gacaagaaaa tcacccacca 2160ctggcatagg agtggtagca ccatcggaaa ggcatttgag gccactgtga gaggcgccaa 2220gagaatggca gtcctggggg atacagcctg ggacttcgga tcagtcgggg gtgtgttcaa 2280ctcactgggt aagggcattc accagatttt tggagcagcc ttcaaatcac tgtttggagg 2340aatgtcctgg ttctcacaga tcctcatagg cacgctgcta gtgtggttag gtttgaacac 2400aaagaatgga tctatctccc tcacatgctt ggccctgggg ggagtgatga tcttcctctc 2460cacggctgtt tctgctgacg tggggtgctc agtggacttc tcaaaaaagg aaacgagatg 2520tggcacgggg gtattcatct ataatgatgt tgaagcctgg agggaccggt acaagtacca 2580tcctgactcc ccccgcagat tggcagcagc agtcaagcag gcctgggaag aggggatctg 2640tgggatctca tccgtttcaa gaatggaaaa catcatgtgg aaatcagtag aaggggagct 2700caatgctatc ctagaggaga atggagttca actgacagtt gttgtgggat ctgtaaaaaa 2760ccccatgtgg agaggtccac aaagattgcc agtgcctgtg aatgagctgc cccatggctg 2820gaaagcctgg gggaaatcgt attttgttag ggcggcaaag accaacaaca gttttgttgt 2880cgacggtgac acactgaagg aatgtccgct tgagcacaga gcatggaata gttttcttgt 2940ggaggatcac gggtttggag tcttccacac cagtgtctgg cttaaggtca gagaagatta 3000ctcattagaa tgtgacccag ccgtcatagg aacagctgtt aagggaaggg aggccgcgca 3060cagtgatctg ggctattgga ttgaaagtga aaagaatgac acatggaggc tgaagagggc 3120ccacctgatt gagatgaaaa catgtgaatg gccaaagtct cacacattgt ggacagatgg 3180agtagaagaa agtgatctta tcatacccaa gtctttagct ggtccactca gccaccacaa 3240caccagagag ggttacagaa cccaagtgaa agggccatgg cacagtgaag agcttgaaat 3300ccggtttgag gaatgtccag gcaccaaggt ttacgtggag gagacatgcg gaactagagg 3360accatctctg agatcaacta ctgcaagtgg aagggtcatt gaggaatggt gctgtaggga 3420atgcacaatg cccccactat cgtttcgagc aaaagacggc tgctggtatg gaatggagat 3480aaggcccagg aaagaaccag agagcaactt agtgaggtca atggtgacag cggggtcaac 3540cgatcatatg gaccacttct ctcttggagt gcttgtgatt ctactcatgg tgcaggaggg 3600gttgaagaag agaatgacca caaagatcat catgagcaca tcaatggcag tgctggtagt 3660catgatcttg ggaggatttt caatgagtga cctggccaag cttgtgatcc tgatgggtgc 3720tactttcgca gaaatgaaca ctggaggaga tgtagctcac ttggcattgg tagcggcatt 3780taaagtcaga ccagccttgc tggtctcctt cattttcaga gccaattgga caccccgtga 3840gagcatgctg ctagccctgg cttcgtgtct tctgcaaact gcgatctctg ctcttgaagg 3900tgacttgatg gtcctcatta atggatttgc tttggcctgg ttggcaattc gagcaatggc 3960cgtgccacgc actgacaaca tcgctctacc aatcttggct gctctaacac cactagctcg 4020aggcacactg ctcgtggcat ggagagcggg cctggctact tgtggaggga tcatgctcct 4080ctccctgaaa gggaaaggta gtgtgaagaa gaacctgcca tttgtcatgg ccctgggatt 4140gacagctgtg agggtagtag accctattaa tgtggtagga ctactgttac tcacaaggag 4200tgggaagcgg agctggcccc ctagtgaagt tctcacagcc gttggcctga tatgtgcact 4260ggccggaggg tttgccaagg cagacattga gatggctgga cccatggctg cagtaggctt 4320gctaattgtc agctatgtgg tctcgggaaa gagtgtggac atgtacattg aaagagcagg 4380tgacatcaca tgggaaaagg acgcggaagt cactggaaac agtcctcggc ttgacgtggc 4440actggatgag agtggtgact tctccttggt agaggaagat ggtccaccca tgagagagat 4500catactcaag gtggtcctga tggccatctg tggcatgaac ccaatagcta taccttttgc 4560tgcaggagcg tggtatgtgt atgtgaagac tgggaaaagg agtggcgccc tctgggacgt 4620gcctgctccc aaagaagtga agaaaggaga gaccacagat ggagtgtaca gagtgatgac 4680tcgcagactg ctaggttcaa cacaggttgg agtgggagtc atgcaagagg gagtcttcca 4740caccatgtgg cacgttacaa aaggagccgc actgaggagc ggtgagggaa gacttgatcc 4800atactggggg gatgtcaagc aggacttggt gtcatactgt gggccttgga agttggatgc 4860agcttgggat ggactcagcg aggtacagct tttggccgta cctcccggag agagggccag 4920aaacattcag accctgcctg gaatattcaa gacaaaggac ggggacatcg gagcagttgc 4980tctggactac cctgcaggga cctcaggatc tccgatccta gacaaatgtg gaagagtgat 5040aggactctat ggcaatgggg ttgtgatcaa gaatggaagc tatgttagtg ctataaccca 5100gggaaagagg gaggaggaga ctccggttga atgtttcgaa ccctcgatgc tgaagaagaa 5160gcagctaact gtcttggatc tgcatccagg agccggaaaa accaggagag ttcttcctga 5220aatagtccgt gaagccataa aaaagagact ccggacagtg atcttggcac caactagggt 5280tgtcgctgct gagatggagg aggccttgag aggacttccg gtgcgttaca tgacaacagc 5340agtcaacgtc acccattctg ggacagaaat cgttgatttg atgtgccatg ccactttcac 5400ttcacgctta ctacaaccca tcagagtccc taattacaat ctcaacatca tggatgaagc 5460ccacttcaca gacccctcaa gtatagctgc aagaggatac atatcaacaa gggttgaaat 5520gggcgaggcg gctgccattt ttatgactgc cacaccacca ggaacccgtg atgcgtttcc 5580tgactctaac tcaccaatca tggacacaga agtggaagtc ccagagagag cctggagctc 5640aggctttgat tgggtgacag accattctgg gaaaacagtt tggttcgttc caagcgtgag 5700aaacggaaat gaaatcgcag cctgtctgac aaaggctgga aagcgggtca tacagctcag 5760caggaagact tttgagacag aatttcagaa aacaaaaaat caagagtggg actttgtcat 5820aacaactgac atctcagaga tgggcgccaa cttcaaggct gaccgggtca tagactctag 5880gagatgccta aaaccagtca tacttgatgg tgagagagtc atcttggctg ggcccatgcc 5940tgtcacgcat gctagtgctg ctcagaggag aggacgtata ggcaggaacc ctaacaaacc 6000tggagatgag tacatgtatg gaggtgggtg tgcagagact gatgaaggcc atgcacactg 6060gcttgaagca agaatgcttc ttgacaacat ctacctccag gatggcctca tagcctcgct 6120ctatcggcct gaggccgata aggtagccgc cattgaggga gagtttaagc tgaggacaga 6180gcaaaggaag accttcgtgg aactcatgaa gagaggagac cttcccgtct ggctagccta 6240tcaggttgca tctgccggaa taacttacac agacagaaga tggtgctttg atggcacaac 6300caacaacacc ataatggaag acagtgtacc agcagaggtt tggacaaagt atggagagaa 6360gagagtgctc aaaccgagat ggatggatgc tagggtctgt tcagaccatg cggccctgaa 6420gtcgttcaaa gaattcgccg ctggaaaaag aggagcggct ttgggagtaa tggaggccct 6480gggaacactg ccaggacaca tgacagagag gtttcaggaa gccattgaca acctcgccgt 6540gctcatgcga gcagagactg gaagcaggcc ttataaggca gcggcagccc aactgccgga 6600gaccctagag accattatgc tcttaggttt gctgggaaca gtttcactgg ggatcttctt 6660cgtcttgatg cggaataagg gcatcgggaa gatgggcttt ggaatggtaa cccttggggc 6720cagtgcatgg ctcatgtggc tttcggaaat tgaaccagcc agaattgcat gtgtcctcat 6780tgttgtgttt ttattactgg tggtgctcat acccgagcca gagaagcaaa gatctcccca 6840agataaccag atggcaatta tcatcatggt ggcagtgggc cttctaggtt tgataactgc 6900aaacgaactt ggatggctgg aaagaacaaa aaatgacata gctcatctaa tgggaaggag 6960agaagaagga gcaaccatgg gattctcaat ggacattgat ctgcggccag cctccgcctg 7020ggctatctat gccgcattga caactctcat caccccagct gtccaacatg cggtaaccac 7080ttcatacaac aactactcct taatggcgat ggccacacaa gctggagtgc tgtttggcat 7140gggcaaaggg atgccattta tgcatgggga ccttggagtc ccgctgctaa tgatgggttg 7200ctattcacaa ttaacacccc tgactctgat agtagctatc attctgcttg tggcgcacta 7260catgtacttg atcccaggcc tacaagcggc agcagcgcgt gctgcccaga aaaggacagc 7320agctggcatc atgaagaatc ccgttgtgga tggaatagtg gtaactgaca ttgacacaat 7380gacaatagac ccccaggtgg agaagaagat gggacaagtg ttactcatag cagtagccat 7440ctccagtgct gtgctgctgc ggaccgcctg gggatggggg gaggctggag ctctgatcac 7500agcagcgacc tccaccttgt gggaaggctc tccaaacaaa tactggaact cctctacagc 7560cacctcactg tgcaacatct tcagaggaag ctatctggca ggagcttccc ttatctatac 7620agtgacgaga aacgctggcc tggttaagag acgtggaggt gggacgggag agactctggg 7680agagaagtgg aaagctcgtc tgaatcagat gtcggccctg gagttctact cttataaaaa 7740gtcaggtatc actgaagtgt gtagagagga ggctcgccgt gccctcaagg atggagtggc 7800cacaggagga catgccgtat cccggggaag tgcaaagatc agatggttgg aggagagagg 7860atatctgcag ccctatggga aggttgttga cctcggatgt ggcagagggg gctggagcta 7920ttatgccgcc accatccgca aagtgcagga ggtgagagga tacacaaagg gaggtcccgg 7980tcatgaagaa cccatgctgg tgcaaagcta tgggtggaac atagttcgtc tcaagagtgg 8040agtggacgtc ttccacatgg cggctgagcc gtgtgacact ctgctgtgtg acataggtga 8100gtcatcatct agtcctgaag tggaagagac acgaacactc agagtgctct ctatggtggg 8160ggactggctt gaaaaaagac caggggcctt ctgtataaag gtgctgtgcc catacaccag 8220cactatgatg gaaaccatgg agcgactgca acgtaggcat gggggaggat tagtcagagt 8280gccattgtgt cgcaactcca cacatgagat gtactgggtc tctggggcaa agagcaacat 8340cataaaaagt gtgtccacca caagtcagct cctcctggga cgcatggatg gccccaggag 8400gccagtgaaa tatgaggagg atgtgaacct cggctcgggt acacgagctg tggcaagctg 8460tgctgaggct cctaacatga aaatcatcgg caggcgcatt gagagaatcc gcaatgaaca 8520tgcagaaaca tggtttcttg atgaaaacca cccatacagg acatgggcct accatgggag 8580ctacgaagcc cccacgcaag gatcagcgtc ttccctcgtg aacggggttg ttagactcct 8640gtcaaagcct tgggacgtgg tgactggagt tacaggaata gccatgactg acaccacacc 8700atacggccaa caaagagtct tcaaagaaaa agtggacacc agggtgccag atccccaaga 8760aggcactcgc caggtaatga acatagtctc ttcctggctg tggaaggagc tggggaaacg 8820caagcggcca cgcgtctgca ccaaagaaga gtttatcaac aaggtgcgca gcaatgcagc 8880actgggagca atatttgaag aggaaaaaga atggaagacg gctgtggaag ctgtgaatga 8940tccaaggttt tgggccctag tggataggga gagagaacac cacctgagag gagagtgtca 9000cagctgtgtg tacaacatga tgggaaaaag agaaaagaag caaggagagt tcgggaaagc 9060aaaaggtagc cgcgccatct ggtacatgtg gttgggagcc agattcttgg agtttgaagc 9120ccttggattc ttgaacgagg accattggat gggaagagaa aactcaggag gtggagtcga 9180agggttagga ttgcaaagac ttggatacat tctagaagaa atgaatcggg caccaggagg 9240aaagatgtac gcagatgaca ctgctggctg ggacacccgc attagtaagt ttgatctgga 9300gaatgaagct ctgattacca accaaatgga ggaagggcac agaactctgg cgttggccgt 9360gattaaatac acataccaaa acaaagtggt gaaggttctc agaccagctg aaggaggaaa 9420aacagttatg gacatcattt caagacaaga ccagagaggg agtggacaag ttgtcactta 9480tgctctcaac acattcacca acttggtggt gcagcttatc cggaacatgg aagctgagga 9540agtgttagag atgcaagact tatggttgtt gaggaagcca gagaaagtga ccagatggtt 9600gcagagcaat ggatgggata gactcaaacg aatggcggtc agtggagatg actgcgttgt 9660gaagccaatc gatgataggt ttgcacatgc cctcaggttc ttgaatgaca tgggaaaagt 9720taggaaagac acacaggagt ggaaaccctc gactggatgg agcaattggg aagaagtccc 9780gttctgctcc caccacttca acaagctgta cctcaaggat gggagatcca ttgtggtccc 9840ttgccgccac caagatgaac tgattggccg agctcgcgtc tcaccagggg caggatggag 9900catccgggag actgcctgtc ttgcaaaatc atatgcgcag atgtggcagc tcctttattt 9960ccacagaaga gaccttcgac tgatggctaa tgccatttgc tcggctgtgc cagttgactg 10020ggtaccaact gggagaacca cctggtcaat ccatggaaag ggagaatgga tgaccactga 10080ggacatgctc atggtgtgga atagagtgtg gattgaggag aacgaccata tggaggacaa 10140gactcctgta acaaaatgga cagacattcc ctatctagga aaaagggagg acttatggtg 10200tggatccctt atagggcaca gaccccgcac cacttgggct gaaaacatca aagacacagt 10260caacatggtg cgcaggatca taggtgatga agaaaagtac atggactatc tatccaccca 10320agtccgctac ttgggtgagg aagggtccac acccggagtg ttgtaagcac caattttagt 10380gttgtcaggc ctgctagtca gccacagttt ggggaaagct gtgcagcctg taaccccccc 10440aggagaagct gggaaaccaa gctcatagtc aggccgagaa cgccatggca cggaagaagc 10500catgctgcct gtgagcccct cagaggacac tgagtcaaaa aaccccacgc gcttggaagc 10560gcaggatggg aaaagaaggt ggcgaccttc cccacccttc aatctggggc ctgaactgga 10620gactagctgt gaatctccag cagagggact agtggttaga ggagaccccc cggaaaacgc 10680aaaacagcat attgacgtgg gaaagaccag agactccatg agtttccacc acgctggccg 10740ccaggcacag atcgccgaac ttcggcggcc ggtgtgggga aatccatggt ttct 107941310617DNAZika virus 13agtatcaaca ggttttattt tggatttgga aacgagagtt tctggtcatg aaaaacccaa 60aaaagaaatc cggaggattc cggattgtca atatgctaaa acgcggagta gcccgtgtga 120gcccctttgg gggcttgaag aggctgccag ccggacttct gctgggtcat gggcccatca 180ggatggtctt ggcgattcta gcctttttga gattcacggc aatcaagcca tcactgggtc 240tcatcaatag atggggttca gtggggaaaa aagaggctat ggaaataata aagaagttca 300agaaagatct ggctgccatg ctgagaataa tcaatgctag gaaggagaag aagagacgag 360gcgcagatac tagtgtcgga attgttggcc tcctgctgac cacagctatg gcagcggagg 420tcactagacg tgggagtgca tactatatgt acttggacag aaacgacgct ggggaggcca 480tatcttttcc aaccacattg gggatgaata agtgttatat acagatcatg gatcttggac 540acatgtgtga tgccaccatg agctatgaat gccctatgct ggatgagggg gtggaaccag 600atgacgtcga ttgttggtgc aacacgacgt caacttgggt tgtgtacgga acctgccatc 660acaaaaaagg tgaagcacgg agatctagaa gagctgtgac gctcccctcc cattccacta 720ggaagctgca aacgcggtcg caaacctggt tggaatcaag agaatacaca aagcacttga 780ttagagtcga aaattggata ttcaggaacc ctggcttcgc gttagcagca gctgccatcg 840cttggctttt gggaagctca acgagccaaa aagtcatata cttggtcatg atactgctga 900ttgccccggc atacagcatc aggtgcatag gagtcagcaa tagggacttt gtggaaggta 960tgtcaggtgg gacttgggtt gatgttgtct tggaacatgg aggttgtgtc accgtaatgg 1020cacaggacaa accgactgtc gacatagagc tggttacaac aacagtcagc aacatggcgg 1080aggtaagatc ctactgctat gaggcatcaa tatcggacat ggcttcggac agccgctgcc 1140caacacaagg tgaagcctac cttgacaagc aatcagacac tcaatatgtc tgcaaaagaa 1200cgttagtgga cagaggctgg ggaaatggat gtggactttt tggcaaaggg agcctggtga 1260catgcgctaa gtttgcatgc tccaagaaaa tgaccgggaa gagcatccag ccagagaatc 1320tggagtaccg gataatgctg tcagttcatg gctcccagca cagtgggatg atcgttaatg 1380acacaggaca tgaaactgat gagaatagag cgaaggttga gataacgccc aattcaccaa 1440gagccgaagc caccctgggg ggttttggaa gcctaggact tgattgtgaa ccgaggacag 1500gccttgactt ttcagatttg tattacttga ctatgaataa caagcactgg ttggttcaca

1560aggagtggtt ccacgacatt ccattacctt ggcacgctgg ggcagacacc ggaactccac 1620actggaacaa caaagaagca ctggtagagt tcaaggacgc acatgccaaa aggcaaactg 1680tcgtggttct agggagtcaa gaaggagcag ttcacacggc ccttgctgga gctctggagg 1740ctgagatgga tggtgcaaag ggaaggctgt cctctggcca cttgaaatgt cgcctgaaaa 1800tggataaact tagattgaag ggcgtgtcat actccttgtg taccgcagcg ttcacattca 1860ccaagatccc ggctgaaaca ctgcacggga cagtcacagt ggaggtacag tacgcaggga 1920cagatggacc ttgcaaggtt ccagctcaga tggcggtgga catgcaaact ctgaccccag 1980ttgggaggtt gataaccgct aaccccgtaa tcactgaaag cactgagaac tctaagatga 2040tgctggaact tgatccacca tttggggact cttacattgt cataggagtc ggggagaaga 2100agatcaccca ccactggcac aggagtggca gcaccattgg aaaagcattt gaagccactg 2160tgagaggtgc caagagaatg gcagtcttgg gagacacagc ctgggacttt ggatcagttg 2220gaggcgctct caactcattg ggcaagggca tccatcaaat ttttggagca gctttcaaat 2280cattgtttgg aggaatgtcc tggttctcac aaattctcat tggaacgttg ctgatgtggt 2340tgggtctgaa cacaaagaat ggatctattt cccttatgtg cttggcctta gggggagtgt 2400tgatcttctt atccacagct gtctctgctg atgtggggtg ctcggtggac ttctcaaaga 2460aggagacgag atgcggtaca ggggtgttcg tctataacga cgttgaagcc tggagggaca 2520ggtacaagta ccatcctgac tccccccgta gattggcagc agcagtcaag caagcctggg 2580aagatggtat ctgtgggatc tcctctgttt caagaatgga aaacatcatg tggagatcag 2640tagaagggga gctcaacgca atcctggaag agaatggagt tcaactgacg gtcgttgtgg 2700gatctgtaaa aaaccccatg tggagaggtc cacagagatt gcccgtgcct gtgaacgagc 2760tgccccacgg ctggaaggct tgggggaaat cgtacttcgt cagagcagca aagacaaata 2820acagctttgt cgtggatggt gacacactga aggaatgccc actcaaacat agagcatgga 2880acagctttct tgtggaggat catgggttcg gggtatttca cactagtgtc tggctcaagg 2940ttagagaaga ttattcatta gagtgtgatc cagccgttat tggaacagct gttaagggaa 3000aggaggctgt acacagtgat ctaggctact ggattgagag tgagaagaat gacacatgga 3060ggctgaagag ggcccatctg atcgagatga aaacatgtga atggccaaag tcccacacat 3120tgtggacaga tggaatagaa gagagtgatc tgatcatacc caagtcttta gctgggccac 3180tcagccatca caataccaga gagggctaca ggacccaaat gaaagggcca tggcacagtg 3240aagagcttga aattcggttt gaggaatgcc caggcactaa ggtccacgtg gaggaaacat 3300gtggaacaag aggaccatct ctgagatcaa ccactgcaag cggaagggtg atcgaggaat 3360ggtgctgcag ggagtgcaca atgcccccac tgtcgttccg ggctaaagat ggctgttggt 3420atggaatgga gataaggccc aggaaagaac cagaaagtaa cttagtaagg tcaatggtga 3480ctgcaggatc aactgatcac atggatcact tctcccttgg agtgcttgtg attctgctca 3540tggtgcagga agggctgaag aagagaatga ccacaaagat catcataagc acatcgatgg 3600cagtgctggt agctatgatc ctgggaggat tttcaatgag tgacctggct aagcttgcaa 3660ttttgatggg tgccaccttc gcggaaatga acactggagg agatgtagct catctggcgc 3720tgatagcggc attcaaagtc agaccagcgt tgctggtatc tttcatcttc agagctaatt 3780ggacaccccg tgaaagcatg ctgctggcct tggcctcgtg tcttttgcaa actgcgatct 3840ccgccttgga aggcgacctg atggttctca tcaatggttt tgctttggcc tggttggcaa 3900tacgagcgat ggttgttcca cgcactgata acatcacctt ggcaatcctg gctgctctga 3960caccactggc ccggggcaca ctgcttgtgg cgtggagagc aggccttgct acttgcgggg 4020ggtttatgct cctctctctg aagggaaaag gcagtgtgaa gaagaactta ccatttgtca 4080tggccctggg actaaccgct gtgaggctgg tcgaccccat caacgtggtg ggactgctgt 4140tgctcacaag gagtgggaag cggagctggc cccctagcga agtactcaca gctgttggcc 4200tgatatgcgc attggctgga gggttcgcca aggcagatat agagatggct gggcccatgg 4260ccgcggtcgg tctgctaatt gtcagttacg tggtctcagg aaagagtgtg gacatgtaca 4320ttgaaagagc aggtgacatc acatgggaaa aagatgcgga agtcactgga aacagtcccc 4380ggctcgatgt ggcgctagat gagagtggtg atttctccct ggtggaggat gacggtcccc 4440ccatgagaga gatcatactc aaggtggtcc tgatgaccat ctgtggcatg aacccaatag 4500ccataccctt tgcagctgga gcgtggtacg tatacgtgaa gactggaaaa aggagtggtg 4560ctctatggga tgtgcctgct cccaaggaag taaaaaaggg ggagaccaca gatggagtgt 4620acagagtaat gactcgtaga ctgctaggtt caacacaagt tggagtggga gttatgcaag 4680agggggtctt tcacactatg tggcacgtca caaaaggatc cgcgctgaga agcggtgaag 4740ggagacttga tccatactgg ggagatgtca agcaggatct ggtgtcatac tgtggtccat 4800ggaagctaga tgccgcctgg gacgggcaca gcgaggtgca gctcttggcc gtgccccccg 4860gagagagagc gaggaacatc cagactctgc ccggaatatt taagacaaag gatggggaca 4920ttggagcggt tgcgctggat tacccagcag gaacttcagg atctccaatc ctagacaagt 4980gtgggagagt gataggactt tatggcaatg gggtcgtgat caaaaatggg agttatgtta 5040gtgccatcac ccaagggagg agggaggaag agactcctgt tgagtgcttc gagccttcga 5100tgctgaagaa gaagcagcta actgtcttag acttgcatcc tggagctggg aaaaccagga 5160gagttcttcc tgaaatagtc cgtgaagcca taaaaacaag actccgtact gtgatcttag 5220ctccaaccag ggttgtcgct gctgaaatgg aggaagccct tagagggctt ccagtgcgtt 5280atatgacaac agcagtcaat gtcacccact ctggaacaga aatcgtcgac ttaatgtgcc 5340atgccacctt cacttcacgt ctactacagc caatcagagt ccccaactat aatctgtata 5400ttatggatga ggcccacttc acagatccct caagtatagc agcaagagga tacatttcaa 5460caagggttga gatgggcgag gcggctgcca tcttcatgac cgccacgcca ccaggaaccc 5520gtgacgcatt tccggactcc aactcaccaa ttatggacac cgaagtggaa gtcccagaga 5580gagcctggag ctcaggcttt gattgggtga cggatcattc tggaaaaaca gtttggtttg 5640ttccaagcgt gaggaacggc aatgagatcg cagcttgtct gacaaaggct ggaaaacggg 5700tcatacagct cagcagaaag acttttgaga cagagttcca gaaaacaaaa catcaagagt 5760gggactttgt cgtgacaact gacatttcag agatgggcgc caactttaaa gctgaccgtg 5820tcatagattc caggagatgc ctaaagccgg tcatacttga tggcgagaga gtcattctgg 5880ctggacccat gcctgtcaca catgccagcg ctgcccagag gagggggcgc ataggcagga 5940atcccaacaa acctggagat gagtatctgt atggaggtgg gtgcgcagag actgacgaag 6000accatgcaca ctggcttgaa gcaagaatgc tccttgacaa tatttacctc caagatggcc 6060tcatagcctc gctctatcga cctgaggccg acaaagtagc agccattgag ggagagttca 6120agcttaggac ggagcaaagg aagacctttg tggaactcat gaaaagagga gatcttcctg 6180tttggctggc ctatcaggtt gcatctgccg gaataaccta cacagataga agatggtgct 6240ttgatggcac gaccaacaac accataatgg aagacagtgt gccggcagag gtgtggacca 6300gacacggaga gaaaagagtg ctcaaaccga ggtggatgga cgccagagtt tgttcagatc 6360atgcggccct gaagtcattc aaggagtttg ccgctgggaa aagaggagcg gcttttggag 6420tgatggaagc cctgggaaca ctgccaggac acatgacaga gagattccag gaagccattg 6480acaacctcgc tgtgctcatg cgggcagaga ctggaagcag gccttacaaa gccgcggcgg 6540cccaattgcc ggagacccta gagaccatta tgcttttggg gttgctggga acagtctcgc 6600tgggaatctt tttcgtcttg atgaggaaca agggcatagg gaagatgggc tttggaatgg 6660tgactcttgg ggccagcgca tggctcatgt ggctctcgga aattgagcca gccagaattg 6720catgtgtcct cattgttgtg ttcctattgc tggtggtgct catacctgag ccagaaaagc 6780aaagatctcc ccaggacaac caaatggcaa tcatcatcat ggtagcagta ggtcttctgg 6840gcttgattac cgccaatgaa ctcggatggt tggagagaac aaagagtgac ctaagccatc 6900taatgggaag gagagaggag ggggcaacca taggattctc aatggacatt gacctgcggc 6960cagcctcagc ttgggccatc tatgctgcct tgacaacttt cattacccca gccgtccaac 7020atgcagtgac cacttcatac aacaactact ccttaatggc gatggccacg caagctggag 7080tgttgtttgg tatgggcaaa gggatgccat tctacgcatg ggactttgga gtcccgctgc 7140taatgatagg ttgctactca caattaacac ccctgaccct aatagtggcc atcattttgc 7200tcgtggcgca ctacatgtac ttgatcccag ggctgcaggc agcagctgcg cgtgctgccc 7260agaagagaac ggcagctggc atcatgaaga accctgttgt ggatggaata gtggtgactg 7320acattgacac aatgacaatt gacccccaag tggagaaaaa gatgggacag gtgctactca 7380tagcagtagc cgtctccagc gccatactgt cgcggaccgc ctgggggtgg ggggaggctg 7440gggccctgat cacagcggca acttccactt tgtgggaagg ctctccgaac aagtactgga 7500actcctctac agccacttca ctgtgtaaca tttttagggg aagttacttg gctggagctt 7560ctctaatcta cacagtaaca agaaacgctg gcttggtcaa gagacgtggg ggtggaacag 7620gagagaccct gggagagaaa tggaaggccc gcttgaacca gatgtcggcc ctggagttct 7680actcctacaa aaagtcaggc atcaccgagg tgtgcagaga agaggcccgc cgcgccctca 7740aggacggtgt ggcaacggga ggccatgctg tgtcccgagg aagtgcaaag ctgagatggt 7800tggtggagcg gggatacctg cagccctatg gaaaggtcat tgatcttgga tgtggcagag 7860ggggctggag ttactacgcc gccaccatcc gcaaagttca agaagtgaaa ggatacacaa 7920aaggaggccc tggtcatgaa gaacccatgt tggtgcaaag ctatgggtgg aacatagtcc 7980gtcttaagag tggggtggac gtctttcata tggcggctga gccgtgtgac acgttgctgt 8040gtgacatagg tgagtcatca tctagtcctg aagtggaaga agcacggacg ctcagagtcc 8100tctccatggt gggggattgg cttgaaaaaa gaccaggagc cttttgtata aaagtgttgt 8160gcccatacac cagcactatg atggaaaccc tggagcgact gcagcgtagg tatgggggag 8220gactggtcag agtgccactc tcccgcaact ctacacatga gatgtactgg gtctctggag 8280cgaaaagcaa caccataaaa agtgtgtcca ccacgagcca gctcctcttg gggcgcatgg 8340acgggcccag gaggccagtg aaatatgagg aggatgtgaa tctcggctct ggcacgcggg 8400ctgtggtaag ctgcgctgaa gctcccaaca tgaagatcat tggtaaccgc attgaaagga 8460tccgcagtga gcacgcggaa acgtggttct ttgacgagaa ccacccatat aggacatggg 8520cttaccatgg aagctatgag gcccccacac aagggtcagc gtcctctcta ataaacgggg 8580ttgtcaggct cctgtcaaaa ccctgggatg tggtgactgg agtcacagga atagccatga 8640ccgacaccac accgtatggt cagcaaagag ttttcaagga aaaagtggac actagggtgc 8700cagaccccca agaaggcact cgtcaggtta tgagcatggt ctcttcctgg ttgtggaaag 8760agctaggcaa acacaaacgg ccacgagtct gtaccaaaga agagttcatc aacaaggttc 8820gtagcaatgc agcattaggg gcaatatttg aagaggaaaa agagtggaag actgcagtgg 8880aagctgtgaa cgatccaagg ttctgggctc tagtggacaa ggaaagagag caccacctga 8940gaggagagtg ccagagttgt gtgtacaaca tgatgggaaa aagagaaaag aaacaagggg 9000aatttggaaa ggccaagggc agccgcgcca tctggtatat gtggctaggg gctagatttc 9060tagagttcga agcccttgga ttcttgaacg aggatcactg gatggggaga gagaactcag 9120gaggtggtgt tgaagggctg ggattacaaa gactcggata tgtcctagaa gagatgagtc 9180gcataccagg aggaaggatg tatgcagatg acactgctgg ctgggacacc cgcatcagca 9240ggtttgatct ggagaatgaa gctctaatca ccaaccaaat ggagaaaggg cacagggcct 9300tggcattggc cataatcaag tacacatacc aaaacaaagt ggtaaaggtc cttagaccag 9360ctgaaaaagg gaagacagtt atggacatta tttcgagaca agaccaaagg gggagcggac 9420aagttgtcac ttacgctctt aacacattta ccaacctagt ggtgcaactc attcggaata 9480tggaggctga ggaagttcta gagatgcaag acttgtggct gctgcggagg tcagagaaag 9540tgaccaactg gttgcagagc aacggatggg ataggctcaa acgaatggca gtcagtggag 9600atgattgcgt tgtgaagcca attgatgata ggtttgcaca tgccctcagg ttcttgaatg 9660atatgggaaa agttaggaag gacacacaag agtggaaacc ctcaactgga tgggacaact 9720gggaagaagt tccgttttgc tcccaccact tcaacaagct ccatctcaag gacgggaggt 9780ccattgtggt tccctgccgc caccaagatg aactgattgg ccgggcccgc gtctctccag 9840gggcgggatg gagcatccgg gagactgctt gcctagcaaa atcatatgcg caaatgtggc 9900agctccttta tttccacaga agggacctcc gactgatggc caatgccatt tgttcatctg 9960tgccagttga ctgggttcca actgggagaa ctacctggtc aatccatgga aagggagaat 10020ggatgaccac tgaagacatg cttgtggtgt ggaacagagt gtggattgag gagaacgacc 10080acatggaaga caagacccca gttacgaaat ggacagacat tccctatttg ggaaaaaggg 10140aagacttgtg gtgtggatct ctcatagggc acagaccgcg caccacctgg gctgagaaca 10200ttaaaaacac agtcaacatg gtgcgcagga tcataggtga tgaagaaaag tacatggact 10260acctatccac ccaagttcgc tacttgggtg aagaagggtc tacacctgga gtgctgtaag 10320caccaatctt agtgttgtca ggcctgctag tcagccacag cttggggaaa gctgtgcagc 10380ctgtgacccc cccaggagaa gctgggaaac caagcctata gtcaggccga gaacgccatg 10440gcacggaaga agccatgctg cctgtgagcc cctcagagga cactgagtca aaaaacccca 10500cgcgcttgga ggcgcaggat gggaaaagaa ggtggcgacc ttccccaccc ttcaatctgg 10560ggcctgaact ggagatcagc tgtggatctc cagaagaggg actagtggtt agaggag 1061714498PRTZika virus 14Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Glu Asn Arg Ala Lys145 150 155 160Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly 165 170 175Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe 180 185 190Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His 195 200 205Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp 210 215 220Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys225 230 235 240Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu 245 250 255Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp 260 265 270Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys 275 280 285Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala 290 295 300Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val305 310 315 320Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro 325 330 335Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu 340 345 350Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met 355 360 365Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly 370 375 380Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr385 390 395 400Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala 405 410 415Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe 420 425 430Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys 435 440 445Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr 450 455 460Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu465 470 475 480Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val 485 490 495Ser Ala15498PRTZika virus 15Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Glu Asn Arg Ala Lys145 150 155 160Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly 165 170 175Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe 180 185 190Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His 195 200 205Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ser Gly Ala Asp 210 215 220Thr Glu Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys225 230 235 240Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu 245 250 255Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp 260 265 270Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys 275 280 285Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala 290 295 300Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val305 310 315 320Thr Val Glu Val Gln Tyr Ala Gly Arg Asp Gly Pro Cys Lys Val Pro 325 330 335Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu 340 345 350Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met 355 360 365Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly 370 375 380Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Ile385 390 395 400Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala 405 410 415Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe 420 425 430Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys 435 440 445Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr 450 455 460Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu465 470 475 480Thr Cys

Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val 485 490 495Ser Ala16504PRTZika virusmisc_feature(156)..(162)X=any amino acid 16Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Xaa Xaa Xaa Xaa Xaa145 150 155 160Xaa Xaa Asn Arg Ala Glu Val Glu Val Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50017504PRTZika virusmisc_feature(152)..(156)X=any amino acid 17Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Xaa Xaa Xaa Xaa Xaa Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Arg Leu Val Arg Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp385 390 395 400Leu Lys Lys Gly Ser Ser Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Val His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50018504PRTZika virus 18Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50019504PRTZika virus 19Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50020504PRTZika virus 20Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys

Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Val His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50021504PRTZika virus 21Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Val His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50022504PRTZika virus 22Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50023504PRTZika virus 23Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Thr Val Asn Asp Ile Gly Tyr Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Lys Leu Phe Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50024504PRTZika virus 24Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Thr Val Asn Asp Ile Gly Tyr Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Lys Leu Phe Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50025504PRTZika virus 25Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly Tyr Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Lys Leu Phe Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser

355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50026504PRTZika virus 26Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Val Cys Thr Ala Ala Lys Val Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50027500PRTZika virus 27Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu Asp Arg145 150 155 160Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu 165 170 175Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu 180 185 190Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu 195 200 205Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly 210 215 220Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu225 230 235 240Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser 245 250 255Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu 260 265 270Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg 275 280 285Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys 290 295 300Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly305 310 315 320Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys 325 330 335Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly 340 345 350Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser 355 360 365Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val 370 375 380Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly385 390 395 400Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg 405 410 415Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly 420 425 430Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala 435 440 445Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile 450 455 460Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile465 470 475 480Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr 485 490 495Ala Val Ser Ala 50028500PRTZika virus 28Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu Asp Arg145 150 155 160Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu 165 170 175Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu 180 185 190Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu 195 200 205Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly 210 215 220Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu225 230 235 240Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser 245 250 255Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu 260 265 270Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg 275 280 285Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys 290 295 300Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly305 310 315 320Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys 325 330 335Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly 340 345 350Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser 355 360 365Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val 370 375 380Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly385 390 395 400Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg 405 410 415Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly 420 425 430Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala 435 440 445Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile 450 455 460Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile465 470 475 480Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr 485 490 495Ala Val Ser Ala 50029500PRTZika virus 29Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu Asp Arg145 150 155 160Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu 165 170 175Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu 180 185 190Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu 195 200 205Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly 210 215 220Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu225 230 235 240Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser 245 250 255Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu 260 265 270Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg 275 280 285Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys 290 295 300Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly305 310 315 320Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys 325 330 335Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly 340 345 350Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser 355 360 365Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val 370 375 380Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly385 390 395 400Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg 405 410 415Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly 420 425 430Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala 435 440 445Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile 450 455 460Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile465 470 475 480Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr 485 490 495Ala Val Ser Ala 50030504PRTZika virus 30Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Thr Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310

315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Gly 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Val Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50031504PRTZika virus 31Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Thr Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Gly 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Val Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50032504PRTZika virus 32Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50033504PRTZika virus 33Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50034504PRTZika virus 34Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50035504PRTZika virus 35Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln

Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50036504PRTZika virus 36Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50037504PRTZika virus 37Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50038504PRTZika virus 38Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50039504PRTZika virus 39Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50040504PRTZika virus 40Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195

200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50041504PRTZika virus 41Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50042504PRTZika virus 42Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50043504PRTZika virus 43Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50044504PRTZika virus 44Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50045504PRTZika virus 45Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp

Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50046504PRTZika virus 46Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50047504PRTZika virus 47Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50048504PRTZika virus 48Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50049504PRTZika virus 49Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50050504PRTZika virus 50Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85

90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50051504PRTZika virus 51Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Ile Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50052504PRTZika virus 52Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Thr Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50053504PRTZika virus 53Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Thr Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50054504PRTZika virus 54Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Arg Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50055504PRTZika virus 55Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro

Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50056504PRTZika virus 56Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Ala Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50057504PRTZika virus 57Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Ala Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50058504PRTZika virus 58Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Thr Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50059504PRTZika virus 59Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Gly Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly

Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50060504PRTZika virus 60Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Leu Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50061504PRTZika virus 61Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Ala Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50062504PRTZika virus 62Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50063504PRTZika virus 63Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50064504PRTZika virus 64Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Ser Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Thr 50 55 60Ile Ser Asp Ile Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Ala Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425

430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50065504PRTZika virusmisc_feature(1)..(504)X=any amino acid 65Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Xaa Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp385 390 395 400Xaa Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50066504PRTZika virus 66Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Ala Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Leu Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Ala His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Ala Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Val Asp Gly Thr Val Thr Val Glu Gly Gln Tyr Gly Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Ile Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Gly 50067504PRTZika virus 67Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Ala Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Leu Val Asn Asp Thr Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Ala His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Ala Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu305 310 315 320Thr Val Asp Gly Thr Val Thr Val Glu Gly Gln Tyr Gly Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Ile Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Gly 50068504PRTZika virus 68Ile Ser Cys Ile Gly Val Ser Asn Arg Asp Leu Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Glu Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Met 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Leu Ser Asp Met Ala Ser Ala Ser Arg Cys Pro Thr Gln Gly Glu Pro65 70 75 80Ser Leu Asp Lys Gln Ser Asp Thr Gln Ser Val Cys Lys Arg Thr Leu 85 90 95Gly Asp Arg Gly Trp Gly Asn Gly Cys Gly Ile Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ser Lys Phe Thr Cys Cys Lys Lys Met Pro Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Pro Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His Glu Thr145 150 155 160Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala 165 170 175Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 180 185 190Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 195 200 205Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 210 215 220Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu225 230 235 240Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 245 250 255Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 260 265 270Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His 275 280 285Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 290 295 300Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu305 310 315 320Thr Leu His Gly Thr Val Thr Val Glu Val Gln Ser Ala Gly Thr Asp 325 330 335Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 340 345 350Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 355 360 365Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 370 375 380Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp385 390 395 400His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 405 410 415Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 420 425 430Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile 435 440 445Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 450 455 460Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys465 470 475 480Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile 485 490 495Phe Leu Ser Thr Ala Val Ser Ala 50069498PRTZika virus 69Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser1 5 10 15Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 20 25 30Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 35 40 45Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 50 55 60Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala65 70 75 80Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 85 90 95Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 100 105 110Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys 115 120 125Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 130 135 140Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Glu Asn Arg Ala Lys145 150 155 160Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly 165 170 175Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe 180 185 190Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His 195 200 205Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp 210 215 220Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys225 230 235 240Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu 245 250 255Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp 260 265 270Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys 275 280 285Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala 290 295 300Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val305 310 315 320Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro 325 330 335Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu 340 345 350Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met 355 360 365Met Leu Glu Leu Asp Pro Pro

Phe Gly Asp Ser Tyr Ile Val Ile Gly 370 375 380Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr385 390 395 400Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala 405 410 415Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe 420 425 430Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys 435 440 445Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr 450 455 460Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu465 470 475 480Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val 485 490 495Ser Ala7026DNAArtificial Sequence1-26 n=inosinemisc_feature(1)..(26)n=inosine 70ncncncncnc ncncncncnc ncncnc 267111PRTArtificial Sequencesynthetic peptide 71Lys Leu Lys Leu Leu Leu Leu Leu Lys Leu Lys1 5 107211840DNAChikungunya virus 72atggctgcgt gagacacacg tagcctacca gtttcttact gctctactct gcaaagcaag 60agattaataa cccatcatgg atcctgtgta cgtggacata gacgctgaca gcgccttttt 120gaaggccctg caacgtgcgt accccatgtt tgaggtggaa ccaaggcagg tcacaccgaa 180tgaccatgct aatgctagag cgttctcgca tctagctata aaactaatag agcaggaaat 240tgaccccgac tcaaccatcc tggatatcgg cagtgcgcca gcaaggagga tgatgtcgga 300caggaagtac cactgcgtct gcccgatgcg cagtgcggaa gatcccgaga gactcgccaa 360ttatgcgaga aagctagcat ctgccgcagg aaaagtcctg gacagaaaca tctctggaaa 420gatcggggac ttacaagcag taatggccgt gccagacacg gagacgccaa cattctgctt 480acacacagac gtctcatgta gacagagagc agacgtcgct atataccaag acgtctatgc 540tgtacacgca cccacgtcgc tataccacca ggcgattaaa ggggtccgag tggcgtactg 600ggttgggttc gacacaaccc cgttcatgta caatgccatg gcgggtgcct acccctcata 660ctcgacaaac tgggcagatg agcaggtact gaaggctaag aacataggat tatgttcaac 720agacctgacg gaaggtagac gaggcaagtt gtctattatg agagggaaaa agctaaaacc 780gtgcgaccgt gtgctgttct cagtagggtc aacgctctac ccggaaagcc gcaagctact 840taagagctgg cacctgccat cggtgttcca tttaaagggc aaactcagct tcacatgccg 900ctgtgataca gtggtttcgt gtgagggcta cgtcgttaag agaataacga tgagcccagg 960cctttatgga aaaaccacag ggtatgcggt aacccaccac gcagacggat tcctgatgtg 1020caagactacc gacacggttg acggcgaaag artgtcattc tcggtgtgca catacgtgcc 1080ggcgaccatt tgtgatcaaa tgaccggcat ccttgctaca gaagtcacgc cggaggatgc 1140acagaagctg ttggtggggc tgaaccagag aatagtggtt aacggcagaa cgcaacggaa 1200tacgaacacc atgaaaaatt atctgcttcc cgtggtcgcc caagccttca gtaagtgggc 1260aaaggagtgc cggaaagaca tggaagatga aaaactcctg ggggtcagag aaagaacact 1320gacctgctgc tgtctatggg cattcaagaa gcagaaaaca cacacggtct acaagaggcc 1380tgatacccag tcaattcaga aggttcaggc cgagtttgac agctttgtgg taccgagtct 1440gtggtcgtcc gggttgtcaa tccctttgag gactagaatc aaatggttgt taagcaaggt 1500gccaaaaacc gacctgatcc catacagcgg agacgcccga gaagcccggg acgcagaaaa 1560agaagcagag gaagaacgag aagcagaact gactcgcgaa gccctaccac ctctacaggc 1620agcacaggaa gatgttcagg tcgaaatcga cgtggaacag cttgaggaca gagcgggcgc 1680aggaataata gagactccga gaggagctat caaagttact gcccaaccaa cagaccacgt 1740cgtgggagag tacctggtac tctccccgca gaccgtacta cgtagccaga agctcagtct 1800gattcacgct ttggcggagc aagtgaagac gtgcacgcac aacggacgag cagggaggta 1860tgcggtcgaa gcgtacgacg gccgagtcct agtgccctca ggctatgcaa tctcgcctga 1920agacttccag agtctaagcg aaagcgcaac gatggtgtat aacgaaagag agttcgtaaa 1980cagaaagcta caccatattg cgatgcacgg accagccctg aacaccgacg aagagtcgta 2040tgagctggtg agggcagaga ggacagaaca cgagtacgtc tacgacgtgg atcagagaag 2100atgctgtaag aaggaagaag ccgcaggact ggtactggtg ggcgacttga ctaatccgcc 2160ctaccacgaa ttcgcatatg aagggctaaa aatccgccct gcctgcccat acaaaattgc 2220agtcatagga gtcttcggag taccgggatc tggcaagtca gctattatca agaacctagt 2280taccaggcag gacctggtga ctagcggaaa gaaagaaaac tgccaagaaa tcaccaccga 2340cgtgatgaga cagagaggtc tagagatatc tgcacgtacg gttgactcgc tgctcttgaa 2400tggatgcaac agaccagtcg acgtgttgta cgtagacgag gcgtttgcgt gccactctgg 2460aacgctactt gctttgatcg ccttggtgag accaaggcag aaagttgtac tttgtggtga 2520cccgaagcag tgcggcttct tcaatatgat gcagatgaaa gtcaactata atcacaacat 2580ctgcacccaa gtgtaccaca aaagtatctc caggcggtgt acactgcctg tgaccgccat 2640tgtgtcatcg ttgcattacg aaggcaaaat gcgcactacg aatgagtaca acaagccgat 2700tgtagtggac actacaggct caacaaaacc tgaccctgga gacctcgtgt taacgtgctt 2760cagagggtgg gttaaacaac tgcaaattga ctatcgtgga tacgaggtca tgacagcagc 2820cgcatcccaa gggttaacca gaaaaggagt ttacgcagtt agacaaaaag ttaatgaaaa 2880cccgctctat gcatcaacgt cagagcacgt caacgtactc ctaacgcgta cggaaggtaa 2940actggtatgg aagacacttt ccggcgaccc gtggataaag acgctgcaga acccaccgaa 3000aggaaacttc aaagcaacta ttaaggagtg ggaggtggag catgcatcaa taatggcggg 3060catctgcagt caccaaatga ccttcgatac attccaaaat aaagccaacg tttgttgggc 3120taagagcttg gtccctatcc tcgaaacagc ggggataaaa ctaaatgata ggcagtggtc 3180tcagataatt caagccttca aagaagacaa agcatactca cctgaagtag ccctgaatga 3240aatatgtacg cgcatgtatg gggtggatct agacagcggg ctattttcta aaccgttggt 3300gtctgtgtat tacgcggata accactggga taataggcct ggagggaaaa tgttcggatt 3360taaccccgag gcagcatcca ttctagaaag aaagtatcca ttcacaaaag ggaagtggaa 3420catcaacaag cagatctgcg tgactaccag gaggatagaa gactttaacc ctaccaccaa 3480catcataccg gccaacagga gactaccaca ctcattagtg gccgaacacc gcccagtaaa 3540aggggaaaga atggaatggc tggttaacaa gataaacggc caccacgtgc tcctggtcag 3600tggctataac cttgcactgc ctactaagag agtcacttgg gtagcgccgt taggtgtccg 3660cggagcggac tacacataca acctagagtt gggtctgcca gcaacgcttg gtaggtatga 3720cctagtggtc ataaacatcc acacaccttt tcgcatacac cattaccaac agtgcgtcga 3780ccacgcaatg aaactgcaaa tgctcggggg tgactcattg agactgctca aaccgggcgg 3840ctctctattg atcagagcat atggttacgc agatagaacc agtgaacgag tcatctgcgt 3900attgggacgc aagtttagat cgtctagagc gttgaaacca ccatgtgtca ccagcaacac 3960tgagatgttt ttcctattca gcaactttga caatggcaga aggaatttca caactcatgt 4020catgaacaat caactgaatg cagccttcgt aggacaggtc acccgagcag gatgtgcacc 4080gtcgtaccgg gtaaaacgca tggacatcgc gaagaacgat gaagagtgcg tagtcaacgc 4140cgctaaccct cgcgggttac cgggtgrcgg tgtttgcaag gcagtataca aaaaatggcc 4200ggagtccttt aagaacagtg caacaccagt gggaaccgca aaaacagtta tgtgcggtac 4260gtatccagta atccacgctg ttggaccaaa cttctctaat tattcggagt ctgaagggga 4320ccgggaattg gcagctgcct atcgagaagt cgcaaaggaa gtaactaggc tgggagtaaa 4380tagtgtagct atacctctcc tctccacagg tgtatactca ggagggaaag acaggctgac 4440ccagtcactg aaccacctct ttacagccat ggactcgacg gatgcagacg tggtcatcta 4500ctgccgcgac aaagaatggg agaagaaaat atctgaggcc atacagatgc ggacccaagt 4560agagctgctg gatgagcaca tctccataga ctgcgatatt gttcgcgtgc accctgacag 4620cagcttggca ggcagaaaag gatacagcac cacggaaggc gcactgtact catatctaga 4680agggacccgt tttcatcaga cggctgtgga tatggcggag atacatacta tgtggccaaa 4740gcaaacagag gccaatgagc aagtctgcct atatgccctg ggggaaagta ttgaatcgat 4800caggcagaaa tgcccggtgg atgatgcaga cgcatcatct ccccccaaaa ctgtcccgtg 4860cctttgccgt tacgctatga ctccagaacg cgtcacccgg cttcgcatga accacgtcac 4920aagcataatt gtgtgttctt cgtttcccct cccaaagtac aaaatagaag gagtgcaaaa 4980agtcaaatgc tctaaggtaa tgctatttga ccacaacgtg ccatcgcgcg taagtccaag 5040ggaatataka tcttcccagg agtctgcaca ggaggcgagt acaatcacgt cactgacgca 5100tagtcaattc gacctaagcg ttgatggcga gatactgccc gtcccgtcag acctggatgc 5160tgacgcccca gccctagaac cagcactaga cgacggggcg acacacacgc tgccatccac 5220aaccggaaac cttgcggccg tgtctgattg ggtaatgagc accgtacctg tcgcgccgcc 5280cagaagaagg cgagggagaa acctgactgt gacatgtgac gagagagaag ggaatataac 5340acccatggct agcgtccgat tctttagggc agagctgtgt ccggtcgtac aagaaacagc 5400ggagacgcgt gacacagcaa tgtctcttca ggcaccaccg agtaccgcca cggaaccgaa 5460tcatccgccg atctccttcg gagcatcaag cgagacgttc cccattacat ttggggactt 5520caacgaagga gaaatcgaaa gcttgtcttc tgagctacta actttcggag acttcttacc 5580aggagaagtg gatgacttga cagacagcga ctggtccacg tgctcagaca cggacgacga 5640gttatgacta gacagggcag gtgggtatat attctcgtcg gacaccggtc caggtcattt 5700acaacagaag tcagtacgcc agtcagtgct gccggtgaac accctggagg aagtccacga 5760ggagaagtgt tacccaccta agctggatga agcaaaggag caactattac ttaagaaact 5820ccaggagagt gcatccatgg ccaacagaag caggtatcag tcgcgcaaag tagaaaacat 5880gaaagcagca atcatccaga gactaaagag aggctgtaga ctatacttaa tgtcagagac 5940cccaaaagtc cctacttacc ggactacata tccggcgcct gtgtactcgc ctccgatcaa 6000cgtccgattg tccaatcccg agtccgcagt ggcagcatgc aatgagttct tagctagaaa 6060ctatccaact gtctcatcat accaaattac cgacgagtat gatgcatatc tagacatggt 6120ggacgggtcg gagagttgcc tggaccgagc gacattcaat ccgtcaaaac tcaggagcta 6180cccgaaacag cacgcttacc acgcgccctc catcagaagc gctgtaccgt ccccattcca 6240gaacacacta cagaatgtac tggcagcagc cacgaaaaga aactgcaacg tcacacagat 6300gagggaatta cccactttgg actcagcagt attcaacgtg gagtgtttca aaaaattcgc 6360atgcaaccaa gaatactggg aagaatttgc tgccagccct attaggataa caactgagaa 6420tttagcaacc tatgttacta aactaaaagg gccaaaagca gcagcgctat tcgcaaaaac 6480ccataatcta ctgccactac aggaagtacc aatggatagg ttcacagtag atatgaaaag 6540ggacgtaaag gtgactcctg gtacaaagca tacagaggaa agacctaagg tgcaggttat 6600acaggcggct gaacccttgg cgacagcata cctatgtggg attcacagag agctggttag 6660gaggctgaac gccgtcctcc tacccaatgt acatacacta tttgacatgt ctgccgagga 6720tttcgatgcc atcatagccg cacactttaa gccaggagac actgttttgg aaacggacat 6780agcctccttt gataagagcc aagatgattc acttgcgctt actgctttga tgctgttaga 6840ggatttaggg gtggatcact ccctgctgga cttgatagag gctgctttcg gagagatttc 6900cagctgtcac ctaccgacag gtacgcgctt caagttcggc gccatgatga aatcaggtat 6960gttcctaact ctgttcgtca acacattgtt aaacatcacc atcgccagcc gagtgctgga 7020agatcgtctg acaaaatccg cgtgcgcggc cttcatcggc gacgacaaca taatacatgg 7080agtcgtctcc gatgaattga tggcagccag atgtgccact tggatgaaca tggaagtgaa 7140gatcatagat gcagttgtat ccttgaaagc cccttacttt tgtggagggt ttatactgca 7200cgatactgtg acaggaacag cttgcagagt ggcagacccg ctaaaaaggc tttttaaact 7260gggcaaaccg ctagcggcag gtgacgaaca agatgaagat agaagacgag cgctggctga 7320cgaagtgatc agatggcaac gaacagggct aattgatgag ctggagaaag cggtatactc 7380taggtacgaa gtgcagggta tatcagttgt ggtaatgtcc atggccacct ttgcaagctc 7440cagatccaac ttcgagaagc tcagaggacc cgtcataact ttgtacggcg gtcctaaata 7500ggtacgcact acagctacct attttgcaga agccgacagc aagtatctaa acactaatca 7560gctacaatgg agttcatccc aacccaaact ttttacaata ggaggtacca gcctcgaccc 7620tggactccgc gccctactat ccaagtcatc aggcccagac cgcgccctca gaggcaagct 7680gggcaacttg cccagctgat ctcagcagtt aataaactga caatgcgcgc ggtaccccaa 7740cagaagccac gcaggaatcg gaagaataag aagcaaaagc aaaaacaaca ggcgccacaa 7800aacaacacaa atcaaaagaa gcagccacct aaaaagaaac cggctcaaaa gaaaaagaag 7860ccgggccgca gagagaggat gtgcatgaaa atcgaaaatg attgtatttt cgaagtcaag 7920cacgaaggta aggtaacagg ttacgcgtgc ctggtggggg acaaagtaat gaaaccagca 7980cacgtaaagg ggaccatcga taacgcggac ctggccaaac tggcctttaa gcggtcatct 8040aagtatgacc ttgaatgcgc gcagataccc gtgcacatga agtccgacgc ttcgaagttc 8100acccatgaga aaccggaggg gtactacaac tggcaccacg gagcagtaca gtactcagga 8160ggccggttca ccatccctac aggtgctggc aaaccagggg acagcggcag accgatcttc 8220gacaacaagg gacgcgtggt ggccatagtc ttaggaggag ctaatgaagg agcccgtaca 8280gccctctcgg tggtgacctg gaataaagac attgtcacta aaatcacccc cgagggggcc 8340gaagagtgga gtcttgccat cccagttatg tgcctgttgg caaacaccac gttcccctgc 8400tcccagcccc cttgcacgcc ctgctgctac gaaaaggaac cggaggaaac cctacgcatg 8460cttgaggaca acgtcatgag acctgggtac tatcagctgc tacaagcatc cttaacatgt 8520tctccccacc gccagcgacg cagcaccaag gacaacttca atgtctataa agccacaaga 8580ccatacttag ctcactgtcc cgactgtgga gaagggcact cgtgccatag tcccgtagca 8640ctagaacgca tcagaaatga agcgacagac gggacgctga aaatccaggt ctccttgcaa 8700atcggaataa agacggatga cagccacgat tggaccaagc tgcgttatat ggacaaccac 8760atgccagcag acgcagagag ggcggggcta tttgtaagaa catcagcacc gtgtacgatt 8820actggaacaa tgggacactt catcctggcc cgatgtccaa aaggggaaac tctgacggtg 8880ggattcactg acagtaggaa gattagtcac tcatgtacgc acccatttca ccacgaccct 8940cctgtgatag gtcgggaaaa attccattcc cgaccgcagc acggtaaaga gctaccttgc 9000agcacgtacg tgcagagcac cgccgcaact accgaggaga tagaggtaca catgccccca 9060gacacccctg atcgcacatt aatgtcacaa cagtccggca acgtaaagat cacagtcaat 9120ggccagacgg tgcggtacaa gtgtaattgc ggtggctcaa atgaaggact aacaactaca 9180gacaaagtga ttaataactg caaggttgat caatgtcatg ccgcggtcac caatcacaaa 9240aagtggcagt ataactcccc tctggtcccg cgtaatgctg aacttgggga ccgaaaagga 9300aaaattcaca tcccgtttcc gctggcaaat gtaacatgca gggtgcctaa agcaaggaac 9360cccaccgtga cgtacgggaa aaaccaagtc atcatgctac tgtatcctga ccacccaaca 9420ctcctgtcct accggaatat gggagaagaa ccaaactatc aagaagagtg ggtgatgcat 9480aagaaggaag tcgtgctaac cgtgccgact gaagggctcg aggtcacgtg gggcaacaac 9540gagccgtata agtattggcc gcagttatct acaaacggta cagcccatgg ccacccgcat 9600gagataattc tgtattatta tgagctgtac cccactatga ctgtagtagt tgtgtcagtg 9660gccacgttca tactcctgtc gatggtgggt atggcagcgg ggatgtgcat gtgtgcacga 9720cgcagatgca tcacaccgta tgaactgaca ccaggagcta ccgtcccttt cctgcttagc 9780ctaatatgct gcatcagaac agctaaagcg gccacatacc aagaggctgc gatatacctg 9840tggaacgagc agcaaccttt gttttggcta caagccctta ttccgctggc agccctgatt 9900gttctatgca actgtctgag actcttacca tgctgctgta aaacgttggc ttttttagcc 9960gtaatgagcg tcggtgccca cactgtgagc gcgtacgaac acgtaacagt gatcccgaac 10020acggtgggag taccgtataa gactctagtc aatagacctg gctacagccc catggtattg 10080gagatggaac tactgtcagt cactttggag ccaacactat cgcttgatta catcacgtgc 10140gagtacaaaa ccgtcatccc gtctccgtac gtgaagtgct gcggtacagc agagtgcaag 10200gacaaaaacc tacctgacta cagctgtaag gtcttcaccg gcgtctaccc atttatgtgg 10260ggcggcgcct actgcttctg cgacgctgaa aacacgcagt tgagcgaagc acacgtggag 10320aagtccgaat catgcaaaac agaatttgca tcagcataca gggctcatac cgcatctgca 10380tcagctaagc tccgcgtcct ttaccaagga aataacatca ctgtaactgc ctatgcaaac 10440ggcgaccatg ccgtcacagt taaggacgcc aaattcattg tggggccaat gtcttcagcc 10500tggacacctt tcgacaacaa aattgtggtg tacaaaggtg acgtctataa catggactac 10560ccgccctttg gcgcaggaag accaggacaa tttggcgata tccaaagtcg cacacctgag 10620agtaaagacg tctatgctaa tacacaactg gtactgcaga gaccggctgt gggtacggta 10680cacgtgccat actctcaggc accatctggc tttaagtatt ggctaaaaga acgcggggcg 10740tcgctgcagc acacagcacc atttggctgc caaatagcaa caaacccggt aagagcggtg 10800aactgcgccg tagggaacat gcccatctcc atcgacatac cggaagcggc cttcactagg 10860gtcgtcgacg cgccctcttt aacggacatg tcgtgcgagg taccagcctg cacccattcc 10920tcagactttg ggggcgtcgc cattattaaa tatgcagcca gcaagaaagg caagtgtgcg 10980gtgcattcga tgactaacgc cgtcactatt cgggaagctg agatagaagt tgaagggaat 11040tctcagctgc aaatctcttt ctcgacggcc ttagccagcg ccgaattccg cgtacaagtc 11100tgttctacac aagtacactg tgcagccgag tgccaccccc cgaaggacca catagtcaac 11160tacccggcgt cacataccac cctcggggtc caggacatct ccgctacggc gatgtcatgg 11220gtgcagaaga tcacgggagg tgtgggactg gttgttgctg ttgccgcact gattctaatc 11280gtggtgctat gcgtgtcgtt cagcaggcac taacttgaca attaagtatg aaggtatatg 11340tgtcccctaa gagacacact gtacatagca aataatctat agatcaaagg gctacgcaac 11400ccctgaatag taacaaaata caaaatcact aaaaattata aaaacagaaa aatacataaa 11460taggtatacg tgtcccctaa gagacacatt gtatgtaggt gataagtata gatcaaaggg 11520ccgaataacc cctgaatagt aacaaaatat gaaaatcaat aaaaatcata aaatagaaaa 11580accataaaca gaagtagttc aaagggctat aaaacccctg aatagtaaca aaacataaaa 11640ttaataaaaa tcaaatgaat accataattg gcaaacggaa gagatgtagg tacttaagct 11700tcctaaaagc agccgaactc actttgagaa gtaggcatag cataccgaac tcttccacga 11760ttctccgaac ccacagggac gtaggagatg ttattttgtt tttaatattt caaaaaaaaa 11820aaaaaaaaaa aaaaaaaaaa 118407310863DNAJapanese encephalitis virus 73tttaaacagt tttttagaac ggaagataac catgactaaa aaaccaggag ggcccggtaa 60aaaccgggct atcaatatgc tgaaacgcgg cctaccccgc gtattcccac tagtgggagt 120gaagagggta gtaatgagct tgttggacgg cagagggcca gtacgtttcg tgctggctct 180tatcacgttc ttcaagttta cagcattagc cccgaccaag gcgcttttag gccgatggaa 240agcagtggaa aagagtgtgg caatgaaaca tcttactagt ttcaaacgag aacttggaac 300actcattgac gccgtgaaca agcggggcag aaagcaaaac aaaagaggag gaaatgaagg 360ctcaatcatg tggctcgcga gcttggcagt tgtcatagct tgtgcaggag ccatgaagtt 420gtcgaatttc caggggaagc ttttgatgac catcaacaac acggacattg cagacgttat 480cgtgattccc acctcaaaag gagagaacag atgctgggtc cgggcaatcg acgtcggcta 540catgtgtgag gacactatca cgtacgaatg tcctaagctt accatgggca atgatccaga 600ggatgtggat tgctggtgtg acaaccaaga agtctacgtc caatatggac ggtgcacgcg 660gaccaggcat tccaagcgaa gcaggagatc cgtgtcggtc caaacacatg gggagagttc 720actagtgaat aaaaaagagg cttggctgga ttcaacgaaa gccacacgat atctcatgaa 780aactgagaac tggatcataa ggaatcctgg ctatgctttc ctggcggcgg tacttggctg 840gatgcttggc agtaacaacg gtcaacgcgt ggtatttacc atcctcctgc tgttggtcgc 900tccggcttac agttttaatt gtctgggaat gggcaatcgt gacttcatag aaggagccag 960tggagccact tgggtggact tggtgctaga aggagatagc tgcttgacaa tcatggcaaa 1020cgacaaacca acattggacg tccgcatgat taacatcgaa gctagccaac ttgctgaggt 1080cagaagttac tgctatcatg cttcagtcac tgacatctcg acggtggctc ggtgccccac 1140gactggagaa gcccacaacg agaagcgagc tgatagtagc tatgtgtgca aacaaggctt 1200cactgaccgt gggtggggca acggatgtgg acttttcggg aagggaagca ttgacacatg 1260tgcaaaattc tcctgcacca gtaaagcgat tgggagaaca atccagccag aaaacatcaa 1320atacgaagtt ggcatttttg tgcatggaac caccacttcg gaaaaccatg ggaattattc 1380agcgcaagtt ggggcgtccc aggcggcaaa gtttacagta acacccaatg ctccttcgat 1440aaccctcaaa cttggtgact acggagaagt cacactggac tgtgagccaa ggagtggact 1500gaacactgaa gcgttttacg tcatgaccgt ggggtcaaag tcatttctgg tccataggga 1560gtggtttcat gacctcgctc tcccctggac gtccccttcg agcacagcgt ggagaaacag 1620agaactcctc atggaatttg aaggggcgca cgccacaaaa cagtccgttg ttgctcttgg 1680gtcacaggaa ggaggcctcc atcaggcgtt ggcaggagcc atcgtggtgg agtactcaag 1740ctcagtgaag ttaacatcag gccacctgaa atgtaggctg aaaatggaca aactggctct 1800gaaaggcaca acctatggca tgtgtacaga aaaattctcg ttcgcgaaaa atccggcgga 1860cactggtcac ggaacagttg tcattgaact ctcctactct gggagtgatg gcccctgcaa 1920aattccgatt gtttccgttg cgagcctcaa tgacatgacc cccgttgggc ggctggtgac 1980agtgaacccc ttcgtcgcga cttccagtgc caactcaaag gtgctggtcg agatggaacc 2040ccccttcgga gactcctaca tcgtagttgg aaggggagac aagcagatca accaccattg

2100gcacaaagct ggaagcacgc tgggcaaggc cttttcaaca actttgaagg gagctcaaag 2160actggcagcg ttgggcgaca cagcctggga ctttggctct attggagggg tcttcaactc 2220cataggaaaa gccgttcacc aagtgtttgg tggtgccttc agaacactct ttgggggaat 2280gtcttggatc acacaagggc taatgggtgc cctactgctc tggatgggcg tcaacgcacg 2340agaccgatca attgctttgg ccttcttagc cacagggggt gtgctcgtgt tcttagcgac 2400caatgtgcat gctgacactg gatgtgccat tgacatcaca agaaaagaga tgagatgtgg 2460aagtggcatc ttcgtgcaca acgacgtgga agcctgggtg gataggtata aatatttgcc 2520agaaacgccc agatccctag cgaagatcgt ccacaaagcg cacaaggaag gcgtgtgcgg 2580agtcagatct gtcactagac tggagcacca aatgtgggaa gccgtacggg acgaattgaa 2640cgtcctgctc aaagagaatg cagtggacct cagtgtggtt gtgaacaagc ccgtgggaag 2700atatcgctca gcccctaaac gcctatccat gacgcaagag aagtttgaaa tgggctggaa 2760agcatgggga aaaagcattc tctttgcccc ggaattggct aactccacat ttgtcgtaga 2820tggacctgag acaaaggaat gccctgatga gcacagagct tggaacagca tgcaaatcga 2880agacttcggc tttggcatca catcaacccg tgtgtggctg aaaattagag aggagagcac 2940tgacgagtgt gatggagcga tcataggcac ggctgtcaaa ggacatgtgg cagtccatag 3000tgacttgtcg tactggattg agagtcgcta caacgacaca tggaaacttg agagggcagt 3060ctttggagag gtcaaatctt gcacttggcc agagacacac accctttggg gagatgatgt 3120tgaggaaagt gaactcatca ttccgcacac catagccgga ccaaaaagca agcacaatcg 3180gagggaaggg tataagacac aaaaccaggg accttgggat gagaatggca tagtcttgga 3240ctttgattat tgcccaggga caaaagtcac cattacagag gattgtggca agagaggccc 3300ttcggtcaga accactactg acagtggaaa gttgatcact gactggtgct gtcgcagttg 3360ctcccttccg cccctacgat tccggacaga aaatggctgc tggtacggaa tggaaatcag 3420acctgttagg catgatgaaa caacactcgt cagatcacag gttgatgctt tcaatggtga 3480aatggttgac ccttttcagc tgggccttct ggtgatgttt ctggccaccc aggaggtcct 3540tcgcaagagg tggacggcca gattgaccat tcctgcggtt ttgggggccc tacttgtgct 3600gatgcttggg ggcatcactt acactgattt ggcgaggtat gtggtgctag tcgctgctgc 3660tttcgcagag gccaacagtg gaggagacgt cctgcacctt gctttgattg ccgtttttaa 3720gatccaacca gcatttctag tgatgaacat gcttagcacg agatggacga accaagaaaa 3780cgtggttctg gtcctagggg ctgccttttt ccaattggcc tcagtagatc tgcaaatagg 3840agtccacgga atcctgaatg ccgccgctat agcatggatg attgtccgag cgatcacctt 3900ccccacaacc tcctccgtca ccatgccagt cttagcgctt ctaactccgg ggatgagggc 3960tctataccta gacacttaca gaatcatcct cctcgtcata gggatttgct ccctgctgca 4020cgagaggaaa aagaccatgg caaaaaagaa aggagctgta ctcttgggct tagcgctcac 4080atccactgga tggttctcgc ccaccactat agctgccgga ctaatggtct gcaacccaaa 4140caagaagaga gggtggccag ctactgagtt tttgtcggca gttggattga tgtttgccat 4200cgtaggtggt ttggccgagt tggatattga atccatgtca atacccttca tgctggcagg 4260tctcatggca gtgtcctacg tggtgtcagg aaaagcaaca gatatgtggc ttgaacgggc 4320cgccgacatc agctgggaga tggatgctgc aatcacagga agcagtcgga ggctggatgt 4380gaaactggat gatgacggag attttcactt gattgatgat cccggtgttc catggaaggt 4440ctgggtcctg cgcatgtctt gcattggctt agccgccctc acgccttggg ccatcgttcc 4500cgccgctttc ggttattggc tcactttaaa aacaacaaaa agagggggcg tgttttggga 4560cacgccatcc ccaaaacctt gctcaaaagg agacaccact acaggagtct accgaattat 4620ggctagaggg attcttggca cttaccaggc cggcgtcgga gtcatgtacg agaatgtttt 4680ccacacacta tggcacacaa ctagaggagc agccattatg agtggagaag gaaaattgac 4740gccatactgg ggtagtgtga gagaagaccg catagcttac ggaggcccat ggaggtttga 4800ccgaaaatgg aatggaacag atgacgtgca agtgatcgtg gtagaaccgg ggaaggctgc 4860agtaaacatc cagacaaaac caggagtgtt tcggactccc ttcggggagg ttggggctgt 4920tagtctggat tacccgcgag gaacatccgg ctcacccatt ctggattcca atggagacat 4980tataggccta tacggcaatg gagttgagct tggcgatggc tcatacgtca gcgccatcgt 5040gcagggtgac cgtcaggagg aaccagtccc agaagcttac accccaaaca tgttgagaaa 5100gagacagatg actgtgctag atttgcaccc tggttcaggg aaaaccagga aaattctgcc 5160acaaataatt aaggacgcta tccagcagcg cctaagaaca gctgtgttgg caccgacgcg 5220ggtggtagca gcagaaatgg cagaagcttt gagagggctc ccagtacgat atcaaacttc 5280agcagtgcag agagagcacc aagggaatga aatagtggat gtgatgtgcc acgccactct 5340gacccataga ctgatgtcac cgaacagagt gcccaactac aacctatttg tcatggatga 5400agctcatttc accgacccag ccagtatagc cgcacgagga tacattgcta ccaaggtgga 5460attaggggag gcagcagcca tctttatgac agcgaccccg cctggaacca cggatccttt 5520tcctgactca aatgccccaa tccatgattt gcaagatgag ataccagaca gggcatggag 5580cagtggatac gaatggatca cagaatatgc gggtaaaacc gtgtggtttg tggcgagcgt 5640aaaaatgggg aatgagattg caatgtgcct ccaaagagcg gggaaaaagg tcatccaact 5700caaccgcaag tcctatgaca cagaataccc aaaatgtaag aatggagact gggattttgt 5760cattaccacc gacatctctg aaatgggggc caacttcggt gcgagcaggg tcatcgactg 5820tagaaagagc gtgaaaccca ccatcttaga agagggagaa ggcagagtca tcctcggaaa 5880cccatctccc ataaccagtg caagcgcagc tcaacggagg ggcagagtag gcagaaaccc 5940caaccaagtt ggagatgaat accactatgg gggggctacc agtgaagatg acagtaacct 6000agcccattgg acagaggcaa agatcatgtt agacaacata cacatgccca atggactggt 6060ggcccagctc tatggaccag agagggaaaa ggctttcaca atggatggcg aataccgtct 6120cagaggtgaa gaaaagaaaa acttcttaga gctgcttagg acggctgacc tcccggtgtg 6180gctggcctac aaggtggcgt ccaatggcat tcagtacacc gacagaaagt ggtgttttga 6240tgggccgcgt acgaatgcca tactggagga caacaccgag gtagagatag tcacccggat 6300gggtgagagg aaaatcctca agccgagatg gcttgatgca agagtttatg cagatcacca 6360agccctcaag tggttcaaag actttgcagc agggaagaga tcagccgtta gcttcataga 6420ggtgctcggt cgcatgcctg agcatttcat gggaaagacg cgggaagctt tagacaccat 6480gtacttggtt gcaacggctg agaaaggtgg gaaagcacac cgaatggctc tcgaagagct 6540gccagatgca ctggaaacca tcacacttat tgtcgccatt actgtgatga caggaggatt 6600cttcctacta atgatgcagc gaaagggtat agggaagatg ggtcttggag ctctagtgct 6660cacgctagct accttcttcc tgtgggcggc agaggttcct ggaaccaaaa tagcagggac 6720cctgctgatc gccctgctgc tgatggtggt tctcatccca gaaccggaaa aacagaggtc 6780acagacagat aaccaactgg cggtgtttct catctgtgtc ttgaccgtgg ttggagtggt 6840ggcagcaaac gagtacggga tgctagaaaa aaccaaagca gatctcaaga gcatgtttgg 6900cggaaagacg caggcatcag gactgactgg attgccaagc atggcactgg acctgcgtcc 6960agccacagcc tgggcactgt atggggggag cacagtcgtg ctaacccctc ttctgaagca 7020cctgatcacg tcggaatacg tcaccacatc gctagcctca attaactcac aagctggctc 7080attattcgtc ttgccacgag gcgtgccttt taccgaccta gacttgaccg ttggcctcgt 7140cttccttggc tgttggggtc aaatcaccct cacaacgttt ctgacagcca tggttctggc 7200gacacttcac tatgggtaca tgctccctgg atggcaagca gaagcactca gggctgccca 7260gagaaggaca gcggctggaa taatgaagaa tgccgttgtt gacggaatgg tcgccactga 7320tgtgcctgaa ctggaaagga ctactcctct gatgcaaaag aaagtcggac aggtgctcct 7380cataggggta agcgtggcag cgttcctcgt caaccctaat gtcaccactg tgagagaagc 7440aggggtgttg gtgacggcgg ctacgcttac tttgtgggac aatggagcca gtgccgtttg 7500gaattccacc acagccacgg gactctgcca tgtcatgcga ggtagctacc tggctggagg 7560ctccattgct tggactctca tcaagaacgc tgataagccc tccttgaaaa ggggaaggcc 7620tgggggcagg acgctagggg agcagtggaa ggaaaaacta aatgccatga gcagagaaga 7680gttttttaaa taccggagag aggccataat cgaggtggac cgcactgaag cacgcagggc 7740cagacgtgaa aataacatag tgggaggaca tccggtttcg cgaggctcag caaaactccg 7800ttggctcgtg gagaaaggat ttgtctcgcc aataggaaaa gtcattgatc tagggtgtgg 7860gcgtggagga tggagctact acgcagcaac cctgaagaag gtccaggaag tcagaggata 7920cacgaaaggt ggggcgggac atgaagaacc gatgctcatg cagagctacg gctggaacct 7980ggtctccctg aagagtggag tggacgtgtt ttacaaacct tcagagccca gtgacaccct 8040gttctgtgac ataggggaat cctccccaag tccagaagta gaagaacaac gcacactacg 8100cgtcctagag atgacatctg actggttgca ccgaggacct agagagttct gcattaaagt 8160tctctgccct tacatgccca aggttataga aaaaatggaa gttctgcagc gccgcttcgg 8220aggtgggcta gtgcgtctcc ccctgtcccg aaactccaat cacgagatgt attgggttag 8280tggagccgct ggcaatgtgg tgcacgctgt gaacatgacc agccaggtac tactggggcg 8340aatggatcgc acagtgtgga gagggccaaa gtatgaggaa gatgtcaacc tagggagcgg 8400aacaagagcc gtgggaaagg gagaagtcca tagcaatcag gagaaaatca agaagagaat 8460ccagaagctt aaagaagaat tcgccacaac gtggcacaaa gaccctgagc atccataccg 8520cacttggaca taccacggaa gctatgaagt gaaggctact ggctcagcca gctctctcgt 8580caacggagtg gtgaagctca tgagcaaacc ttgggacgcc attgccaacg tcaccaccat 8640ggccatgact gacaccaccc cttttggaca gcaaagagtt ttcaaggaga aagttgacac 8700gaaggctcct gagccaccag ctggagccaa ggaagtgctc aacgagacca ccaactggct 8760gtgggcccac ttgtcacggg aaaaaagacc ccgcttgtgc accaaggaag aattcataaa 8820gaaagtcaac agcaacgcgg ctcttggagc agtgttcgct gaacagaatc aatggagcac 8880ggcgcgtgag gctgtggatg acccgcggtt ttgggagatg gttgatgaag agagggaaaa 8940ccatctgcga ggagagtgtc acacatgtat ctacaacatg atgggaaaaa gagagaagaa 9000gcctggagag tttggaaaag ctaaaggaag cagggccatt tggttcatgt ggcttggagc 9060acggtatcta gagtttgaag ctttggggtt cctgaatgaa gaccattggc tgagccgaga 9120gaattcagga ggtggagtgg aaggctcagg cgtccaaaag ctgggataca tcctccgtga 9180catagcagga aagcaaggag ggaaaatgta cgctgatgat accgccgggt gggacactag 9240aattaccaga actgatttag aaaatgaagc taaggtactg gagctcctag acggtgaaca 9300ccgcatgctc gcccgagcca taattgaact gacttacagg cacaaagtgg tcaaggtcat 9360gagacctgca gcagaaggaa agaccgtgat ggacgtgata tcaagagaag atcaaagggg 9420gagtggacag gtggtcactt atgctcttaa cactttcacg aacatcgctg tccagctcgt 9480caggctgatg gaggctgagg gggtcattgg accacaacac ttggaacagc tacctaggaa 9540aaacaagata gctgtcagga cctggctctt tgagaatgga gaggagagag tgaccaggat 9600ggcgatcagc ggagacgact gtgtcgtcaa gccgctggac gacagattcg ccacagccct 9660ccacttcctc aacgcaatgt caaaggtcag aaaagacatc caggaatgga agccttcgca 9720tggctggcac gattggcagc aagttccctt ctgctctaac cattttcagg agattgtgat 9780gaaagatgga aggagtatag ttgtcccgtg cagaggacag gatgagctga taggcagggc 9840tcgcatctct ccaggagctg gatggaatgt gaaggacaca gcttgcctgg ccaaagcata 9900tgcacagatg tggctactcc tatacttcca tcgcagggac ttgcgtctca tggcaaatgc 9960gatttgctca gcagtgccag tggattgggt gcccacaggc aggacatcct ggtcaataca 10020ctcgaaagga gagtggatga ccacggaaga catgctgcag gtctggaaca gagtctggat 10080tgaagaaaat gaatggatga tggacaagac tccaatcaca agctggacag acgttccgta 10140tgtgggaaag cgtgaggaca tctggtgtgg cagcctcatc ggaacgcgat ccagagcaac 10200ctgggctgag aacatctatg cggcgataaa ccaggttaga gctgtcattg ggaaagaaaa 10260ttatgttgac tacatgacct cactcaggag atacgaagac gtcttgatcc aggaagacag 10320ggtcatctag tgtgatttaa ggtagaaaag tagactatgt aaataatgta aatgagaaaa 10380tgcatgcata tggagtcagg ccagcaaaag ctgccaccgg atactgggta gacggtgctg 10440cctgcgtctc agtcccagga ggactgggtt aacaaatctg acaacagaaa gtgagaaagc 10500cctcagaacc gtctcggaag taggtccctg ctcactggaa gttgaaagac caacgtcagg 10560ccacaaattt gtgccactcc gctagggagt gcggcctgcg cagccccagg aggactgggt 10620taccaaagcc gttgaggccc ccacggccca agcctcgtct aggatgcaat agacgaggtg 10680taaggactag aggttagagg agaccccgtg gaaacaacaa catgcggccc aagccccctc 10740gaagctgtag aggaggtgga aggactagag gttagaggag accccgcatt tgcatcaaac 10800agcatattga cacctgggaa tagactggga gatcttctgc tctatctcaa catcagctac 10860tag 108637410977DNAJapanese encephalitis virus 74agaagtttat ctgtgtgaac ttcttggctt agtatcgtag agaagaatcg agagattagt 60gcagtttaaa cagtttttta gaacggaaga taaccatgac taaaaaacca ggagggcccg 120gtaaaaaccg ggctatcaat atgctgaaac gcggcctacc ccgcgtattc ccactagtgg 180gagtgaagag ggtagtaatg agcttgttgg acggcagagg gccagtacgt ttcgtgctgg 240ctcttatcac gttcttcaag tttacagcat tagccccgac caaggcgctt tcaggccgat 300ggaaagcagt ggaaaagagt gtggcaatga aacatcttac tagtttcaaa cgagaacttg 360gaacactcat tgacgccgtg aacaagcggg gcagaaagca aaacaaaaga ggaggaaatg 420aaggctcaat catgtggctc gcgagcttgg cagttgtcat agcttgtgca ggagccatga 480agttgtcgaa tttccagggg aagcttttga tgaccatcaa caacacggac attgcagacg 540ttatcgtgat tcccacctca aaaggagaga acagatgctg ggtccgggca atcgacgtcg 600gctacatgtg tgaggacact atcacgtacg aatgtcctaa gcttaccatg ggcaatgatc 660cagaggatgt ggattgctgg tgtgacaacc aagaagtcta cgtccaatat ggacggtgca 720cgcggaccag gcattccaag cgaagcagga gatccgtgtc ggtccaaaca catggggaga 780gttcactagt gaataaaaaa gaggcttggc tggattcaac gaaagccaca cgatatctca 840tgaaaactga gaactggatc ataaggaatc ctggctatgc tttcctggcg gcggtacttg 900gctggatgct tggcagtaac aacggtcaac gcgtggtatt taccatcctc ctgctgttgg 960tcgctccggc ttacagtttt aattgtctgg gaatgggcaa tcgtgacttc atagaaggag 1020ccagtggagc cacttgggtg gacttggtgc tagaaggaga cagctgcttg acaatcatgg 1080caaacgacaa accaacattg gacgtccgca tgattaacat cgaagctagc caacttgctg 1140aggtcagaag ttactgctat catgcttcag tcactgacat ctcgacggtg gctcggtgcc 1200ccacgactgg agaagcccac aacgagaagc gagctgatag tagctatgtg tgcaaacaag 1260gcttcactga ccgtgggtgg ggcaacggat gtggattttt cgggaaggga agcattgaca 1320catgtgcaaa attctcctgc accagtaaag cgattgggag aacaatccag ccagaaaaca 1380tcaaatacaa agttggcatt tttgtgcatg gaaccaccac ttcggaaaac catgggaatt 1440attcagcgca agttggggcg tcccaggcgg caaagtttac agtaacaccc aatgctcctt 1500cggtagccct caaacttggt gactacggag aagtcacact ggactgtgag ccaaggagtg 1560gactgaacac tgaagcgttt tacgtcatga ccgtggggtc aaagtcattt ctggtccata 1620gggagtggtt tcatgacctc gctctcccct ggacgtcccc ttcgagcaca gcgtggagaa 1680acagagaact cctcatggaa tttgaagggg cgcacgccac aaaacagtcc gttgttgctc 1740ttgggtcaca ggaaggaggc ctccatcatg cgttggcagg agccatcgtg gtggagtact 1800caagctcagt gatgttaaca tcaggccacc tgaaatgtag gctgaaaatg gacaaactgg 1860ctctgaaagg cacaacctat ggcatgtgta cagaaaaatt ctcgttcgcg aaaaatccgg 1920tggacactgg tcacggaaca gttgtcattg aactctccta ctctgggagt gatggcccct 1980gcaaaattcc gattgtttcc gttgcgagcc tcaatgacat gacccccgtt gggcggctgg 2040tgacagtgaa ccccttcgtc gcgacttcca gtgccaactc aaaggtgctg gtcgagatgg 2100aacccccctt cggagactcc tacatcgtag ttggaagggg agacaagcag atcaaccacc 2160attggcacaa agctggaagc acgctgggca aggccttttc aacaactttg aagggagctc 2220aaagactggc agcgttgggc gacacagcct gggactttgg ctctattgga ggggtcttca 2280actccatagg aagagccgtt caccaagtgt ttggtggtgc cttcagaaca ctctttgggg 2340gaatgtcttg gatcacacaa gggctaatgg gtgccctact gctctggatg ggcgtcaacg 2400cacgagaccg atcaattgct ttggccttct tagccacagg aggtgtgctc gtgttcttag 2460cgaccaatgt gcatgctgac actggatgtg ccattgacat cacaagaaaa gagatgagat 2520gtggaagtgg catcttcgtg cacaacgacg tggaagcctg ggtggatagg tataaatatt 2580tgccagaaac gcccagatcc ctagcgaaga tcgtccacaa agcgcacaag gaaggcgtgt 2640gcggagtcag atctgtcact agactggagc accaaatgtg ggaagccgta agggacgaat 2700tgaacgtcct gctcaaagag aatgcagtgg acctcagtgt ggttgtgaac aagcccgtgg 2760gaagatatcg ctcagcccct aaacgcctat ccatgacgca agagaagttt gaaatgggct 2820ggaaagcatg gggaaaaagc atcctctttg ccccggaatt ggctaactcc acatttgtcg 2880tagatggacc tgagacaaag gaatgccctg atgagcacag agcttggaac agcatgcaaa 2940tcgaagactt cggctttggc atcacatcaa cccgtgtgtg gctgaaaatt agagaggaga 3000gcactgacga gtgtgatgga gcgatcatag gcacggctgt caaaggacat gtggcagtcc 3060atagtgactt gtcgtactgg attgagagtc gctacaacga cacatggaaa cttgagaggg 3120cagtctttgg agaggtcaaa tcttgcactt ggccagagac acacaccctt tggggagatg 3180atgttgagga aagtgaactc atcattccgc acaccatagc cggaccaaaa agcaagcaca 3240atcggaggga agggtataag acacaaaacc agggaccttg ggatgagaat ggcatagtct 3300tggactttga ttattgccca gggacaaaag tcaccattac agaggattgt agcaagagag 3360gcccttcggt cagaaccact actgacagtg gaaagttgat cactgactgg tgctgtcgca 3420gttgctccct tccgccccta cgattccgga cagaaaatgg ctgctggtac ggaatggaaa 3480tcagacctgt tatgcatgat gaaacaacac tcgtcagatc acaggttcat gctttcaaag 3540gtgaaatggt tgaccctttt cagctgggcc ttctggtgat gtttctggcc acccaggaag 3600tccttcgcaa gaggtggacg gccagattga ccattcctgc ggttttgggg gtcctacttg 3660tgctgatgct tgggggtatc acttacactg atttggcgag gtatgtggtg ctagtcgctg 3720ctgctttcgc agaggccaac agtggaggag acgtcctgca ccttgctttg attgctgttt 3780ttaagatcca accagcattt ttagtgatga acatgcttag cacgagatgg acgaaccaag 3840aaaacgtggt tctggtccta ggggctgcct ttttccaatt ggcctcagta gatctgcaaa 3900taggagtcca cggaatcctg aatgccgccg ctatagcatg gatgattgtc cgagcgatca 3960ccttccccac aacctcctcc gtcaccatgc cagtcttagc gcttctaact ccggggatga 4020gggctctata cctagacact tacagaatca tcctcctcgt catagggatt tgctccctgc 4080tgcacgagag gaaaaagacc atggcgaaaa agaaaggagc tgtactcttg ggcttagcgc 4140tcacatccac tggatggttc tcgcccacca ctatagctgc cggactaatg gtctgcaacc 4200caaacaagaa gagagggtgg ccagctactg agtttttgtc ggcagttgga ttgatgtttg 4260ccatcgtagg tggtttggcc gagttggata ttgaatccat gtcaataccc ttcatgctgg 4320caggtctcat ggcagtgtcc tacgtggtgt caggaaaagc aacagatatg tggcttgaac 4380gggccgccga catcagctgg gatatgggtg ctgcaatcac aggaagcagt cggaggctgg 4440atgtgaaact ggatgatgac ggagattttc acttgattga tgatcccggt gttccatgga 4500aggtctgggt cctgcgcatg tcttgcattg gcttagccgc cctcacgcct tgggccatcg 4560ttcccgccgc tttcggttat tggctcactt taaaaacaac aaaaagaggg ggcgtgtttt 4620gggacacgcc atccccaaaa ccttgctcaa aaggagacac cactacagga gtctaccgaa 4680ttatggctag agggattctt ggcacttacc aggccggcgt cggagtcatg tacgagaatg 4740ttttccacac actatggcac acaactagag gagcagccat tgtgagtgga gaaggaaaat 4800tgacgccata ctggggtagt gtgaaagaag accgcatagc ttacggaggc ccatggaggt 4860ttgaccgaaa atggaatgga acagatgacg tgcaagtgat cgtggtagaa ccggggaagg 4920gcgcagtaaa catccagaca aaaccaggag tgtttcggac tcccttcggg gaggttgggg 4980ctgttagtct ggattacccg cgaggaacat ccggctcacc cattctggat tccaatggag 5040acattatagg cctatacggc aatggagttg agcttggcga tggctcatac gtcagcgcca 5100tcgtgcaggg tgaccgtcag gaggaaccag tcccagaagc ttacacccca aacatgttga 5160gaaagagaca gatgactgtg ctagatttgc accctggttc agggaaaacc aggaaaattc 5220tgccacaaat aattaaggac gctatccagc agcgcctaag aacagctgtg ttggcaccga 5280cgcgggtggt agcagcagaa atggcagaag ctttgagagg gctcccagta cgatatcaaa 5340cttcagcagt gcagagagag caccaaggga atgaaatagt ggatgtgatg tgccacgcca 5400ctctgaccca tagactgatg tcaccgaaca gagtgcccaa ctacaaccta tttgtcatgg 5460atgaagctca tttcaccgac ccagccagta tagccgcacg aggatacatt gctaccaagg 5520tggaattagg ggaggcagca gccatcttta tgacagcgac cccgcctgga accacggatc 5580cttttcctga ctcaaatgcc ccaatccatg atttgcaaga tgagatacca gacagggcat 5640ggagcagtgg atacgaatgg atcacagaat atgcgggtaa aaccgtgtgg tttgtggcga 5700gcgtaaaaat ggggaatgag attgcaatgt gcctccaaag agcggggaaa aaggtcatcc 5760aactcaaccg caagtcctat gacacagaat acccaaaatg taagaatgga gactgggatt 5820ttgtcattac caccgacatc tctgaaatgg gggccaactt cggtgcgagc agggtcatcg 5880actgtagaaa gagcgtgaaa cccaccatct tagaagaggg agaaggcaga gtcatcctcg 5940gaaacccatc tcccataacc agtgcaagcg cagctcaacg gaggggcaga gtaggcagaa 6000accccaatca agttggagat gaataccact atgggggggc taccagtgaa gatgacagta 6060acctagccca ttggacagag gcaaagatca tgttagacaa catacacatg cccaatggac 6120tggtggccca gctctatgga ccagagaggg aaaaggcttt cacaatggat ggcgaatacc 6180gtctcagagg tgaagaaaag

aaaaacttct tagagctgct taggacggct gacctcccgg 6240tgtggctggc ctacaaggtg gcgtccaatg gcattcagta caccgacaga aagtggtgtt 6300ttgatgggcc gcgtacgaat gccatactgg aggacaacac cgaggtagag atagtcaccc 6360ggatgggtga gaggaaaatc ctcaagccga gatggcttga tgcaagagtt tatgcagatc 6420accaggccct caagtggttc aaagactttg cagcagggaa gagatcagcc gttagcttca 6480tagaggtgct cggtcgcatg cctgagcatt tcatgggaaa gacgcgggaa gctttagaca 6540ccatgtactt ggttgcaacg gctgagaaag gtgggaaagc acaccgaatg gctctcgaag 6600agctgccaga tgcactggaa accatcacac ttattgtcgc cattactgtg atgacaggag 6660gattcttcct actaatgatg cagcgaaagg gtatagggaa gatgggtctt ggagctctag 6720tgctcacact agctaccttc ttcctgtggg cggcagaggt tcctggaacc aaaatagcag 6780ggaccctgct gatcgccctg ctgctgatgg tggttctcat cccagaaccg gaaaaacaga 6840ggtcacagac agataaccaa ctggcggtgt ttctcatctg tgtcttgacc gtggttggag 6900tggtggcagc aaacgagtac gggatgctag aaaaaaccaa agcggatctc aagagcatgt 6960ttggcggaaa gacgcaggca tcaggactga ctggattgcc aagcatggca ctggacctgc 7020gtccagccac agcctgggca ctgtatgggg ggagcacagt cgtgctaacc cctcttctga 7080agcacctgat cacgtcggaa tacgtcacca catcgctagc ttcaattaac tcacaagctg 7140gctcattatt cgtcttgcca cgaggcgtgc cttttaccga cctagacttg actgttggcc 7200tcgtcttcct tggctgttgg ggtcaagtca ccctcacaac gtttctgaca gccatggttc 7260tggcgacact tcactatggg tacatgctcc ctggatggca agcagaagca ctcagggctg 7320cccagagaag gacagcggct ggaataatga agaatgccgt tgttgacgga atggtcgcca 7380ctgatgtgcc tgaactggaa aggactactc ctctgatgca aaagaaagtc ggacaggtgc 7440tcctcatagg ggtaagcgtg gcagcgttcc tcgtcaaccc taatgtcacc actgtgagag 7500aagcaggggt gttggtgacg gcggctacgc ttactttgtg ggacaatgga gccagtgccg 7560tttggaattc caccacagcc acgggactct gccatgtcat gcgaggtagc tacctggctg 7620gaggctccat tgcttggact ctcatcaaga acgctgataa gccctccttg aaaaggggaa 7680ggcctggggg caggacgcta ggggagcagt ggaaggaaaa actaaatgcc atgagtagag 7740aagagttttt taaataccgg agagaggcca taatcgaggt ggaccgcact gaagcacgca 7800gggccagacg tgaaaataac atagtgggag gacatccggt ttcgcgaggc tcagcaaaac 7860tccgttggct cgtggagaaa ggatttgtct cgccaatagg aaaagtcatt gatctagggt 7920gtgggcgtgg aggatggagc tactacgcag caaccctgaa gaaggtccag gaagtcagag 7980gatacacgaa aggtggggcg ggacatgaag aaccgatgct catgcagagc tacggctgga 8040acctggtctc cctgaagagt ggagtggacg tgttttacaa accttcagag cccagtgata 8100ccctgttctg tgacataggg gaatcctccc caagtccaga agtagaagaa caacgcacac 8160tacgcgtcct agagatgaca tctgactggt tgcaccgagg acctagagag ttctgcatta 8220aagttctctg cccttacatg cccaaggtta tagaaaaaat ggaagttctg cagcgtcgct 8280tcggaggtgg gctagtgcgt ctccccctgt cccgaaactc caatcacgag atgtattggg 8340ttagtggagc cgctggcaat gtggtgcacg ctgtgaacat gaccagccag gtattactgg 8400ggcgaatgga tcgcacagtg tggagagggc caaagtatga ggaagatgtc aacctaggga 8460gcggaacaag agccgtggga aagggagaag tccatagcaa tcaggagaaa atcaagaaga 8520gaatccagaa gcttaaagaa gaattcgcca caacgtggca caaagaccct gagcatccat 8580accgcacttg gacataccac ggaagctatg aagtgaaggc tactggctca gccagctctc 8640tcgtcaacgg agtggtgaag ctcatgagca aaccttggga cgccattgcc aacgtcacca 8700ccatggccat gactgacacc accccttttg gacagcaaag agttttcaag gagaaagttg 8760acacgaaggc tcctgagcca ccagctggag ccaaggaagt gctcaacgag accaccaact 8820ggctgtgggc ctacttgtca cgggaaaaaa gaccccgctt gtgcaccaag gaagaattca 8880ttaagaaagt taacagcaac gcggctcttg gagcagtgtt cgctgaacag aatcaatgga 8940gcacggcgcg tgaggctgtg gatgacccgc ggttttggga gatggttgat gaagagaggg 9000aaaaccatct gcgaggagag tgtcacacat gtatctacaa catgatggga aaaagagaga 9060agaagcctgg agagtttgga aaagctaaag gaagcagggc catttggttc atgtggcttg 9120gagcacggta tctagagttt gaagctttgg ggttcctgaa tgaagaccat tggctgagcc 9180gagagaattc aggaggtgga gtggaaggct caggcgtcca aaagctggga tacatcctcc 9240gtgacatagc aggaaagcaa ggagggaaaa tgtacgctga tgataccgcc gggtgggaca 9300ctagaattac cagaactgat ttagaaaatg aagctaaggt actggagctc ctagacggtg 9360aacaccgcat gctcgcccga gccataattg aactgactta caggcacaaa gtggtcaagg 9420tcatgagacc tgcagcagaa ggaaagaccg tgatggacgt gatatcaaga gaagatcaaa 9480gggggagtgg acaggtggtc acttatgctc ttaacacttt cacgaacatc gctgtccagc 9540tcgtcaggct gatggaggct gagggggtca ttggaccaca acacttggaa catctaccta 9600ggaaaaacaa gatagctgtc aggacctggc tctttgagaa tggagaggag agagtgacca 9660ggatggcgat cagcggagac gactgtgccg tcaaaccgct ggacgacaga ttcgccacag 9720ccctccactt cctcaacgca atgtcaaagg tcagaaaaga catccaggaa tggaagcctt 9780cgcatggctg gcacgattgg cagcaagttc ccttctgttc taaccatttt caggagattg 9840tgatgaaaga tggaaggagt atagttgtcc cgtgcagagg acaggatgag ctgataggca 9900gggctcgcat ctctcctgga gctggatgga atgtgaagga cacagcttgc ctggccaaag 9960catatgcaca gatgtggcta ctcctatact tccatcgcag ggacttgcgt ctcatggcaa 10020atgcgatttg ctcagcagtg ccagtagatt gggtgcccac aggcaggaca tcctggtcaa 10080tacactcgaa aggagagtgg atgaccacgg aagacatgct gcaggtctgg aacagagttt 10140ggattgaaga aaatgaatgg atgatggaca agactccaat cacaagctgg acagacgttc 10200cgtatgtggg aaagcgcgag gacatctggt gtggcagcct catcggaacg cgatccagag 10260caacctgggc tgagaacatc tatgcggcga taaaccaggt tagagctgtc attgggaaag 10320aaaattatgt tgactacatg acctcactca ggagatacga agacgtcttg atccaggaag 10380acagggtcat ctagtgtgat ttaaggtaga aaagtagact atgtaaacaa tgtaaatgag 10440aaaatgcatg catatggagt caggccagca aaagctgcca ccggatactg ggtagacggt 10500gctgcctgcg tctcagtccc aggaggactg ggttaacaaa tctgacaaca gaaagtgaga 10560aagccctcag aaccgtctcg gaagtaggtc cctgctcact ggaagttgaa agaccaacgt 10620caggccacaa atttgtgcca ctccgctagg gagtgcggcc tgcgcagccc caggaggact 10680gggttaccaa agccgttgag gcccccacgg cccaagcctc gtctaggatg caatagacga 10740ggtgtaagga ctagaggtta gaggagaccc cgtggaaaca acaacatgcg gcccaagccc 10800cctcgaagct gtagaggagg tggaaggact agaggttaga ggagaccccg catttgcatc 10860aaacagcata ttgacacctg ggaatagact gggagatctt ctgctctatc tcaacatcag 10920ctactaggca cagagcgccg aagtatgtag ctggtggtga ggaagaacac aggatct 109777510976DNAJapanese encephalitis virus 75agaagtttat ctgtgtgaac ttcttggctt agtatcgtag agaagaatcg agagattagt 60gcagtttaaa cagtttttta gaacggaaga taaccatgac taaaaaacca ggagggcccg 120gtaaaaaccg ggctatcaat atgctgaaac gcggcctacc ccgcgtattc ccactagtgg 180gagtgaagag ggtagtaatg agcttgttgg acggcagagg gccagtacgt ttcgtgctgg 240ctcttatcac gttcttcaag tttacagcat tagccccgac caaggcgctt tcaggccgat 300ggaaagcagt ggaaaagagt gtggcaatga aacatcttac tagtttcaaa cgagaacttg 360gaacactcat tgacgccgtg aacaagcggg gcagaaagca aaacaaaaga ggaggaaatg 420aaggctcaat catgtggctc gcgagcttgg cagttgtcat agcttgtgca ggagccatga 480agttgtcgaa tttccagggg aagcttttga tgaccatcaa caacacggac attgcagacg 540ttatcgtgat tcccacctca aaaggagaga acagatgctg ggtccgggca atcgacgtcg 600gctacatgtg tgaggacact atcacgtacg aatgtcctaa gcttaccatg ggcaatgatc 660cagaggatgt ggattgctgg tgtgacaacc aagaagtcta cgtccaatat ggacggtgca 720cgcggaccag gcattccaag cgaagcagga gatccgtgtc ggtccaaaca catggggaga 780gttcactagt gaataaaaaa gaggcttggc tggattcaac gaaagccaca cgatatctca 840tgaaaactga gaactggatc ataaggaatc ctggctatgc tttcctggcg gcggtacttg 900gctggatgct tggcagtaac aacggtcaac gcgtggtatt taccatcctc ctgctgttgg 960tcgctccggc ttacagtttt aattgtctgg gaatgggcaa tcgtgacttc atagaaggag 1020ccagtggagc cacttgggtg gacttggtgc tagaaggaga cagctgcttg acaatcatgg 1080caaacgacaa accaacattg gacgtccgca tgattaacat cgaagctagc caacttgctg 1140aggtcagaag ttactgctat catgcttcag tcactgacat ctcgacggtg gctcggtgcc 1200ccacgactgg agaagcccac aacgagaagc gagctgatag tagctatgtg tgcaaacaag 1260gcttcactga ccgtgggtgg ggcaacggat gtggattttt cgggaaggga agcattgaca 1320catgtgcaaa attctcctgc accagtaaag cgattgggag aacaatccag ccagaaaaca 1380tcaaatacaa agttggcatt tttgtgcatg gaaccaccac ttcggaaaac catgggaatt 1440attcagcgca agttggggcg tcccaggcgg caaagtttac agtaacaccc aatgctcctt 1500cggtagccct caaacttggt gactacggag aagtcacact ggactgtgag ccaaggagtg 1560gactgaacac tgaagcgttt tacgtcatga ccgtggggtc aaagtcattt ctggtccata 1620gggagtggtt tcatgacctc gctctcccct ggacgtcccc ttcgagcaca gcgtggagaa 1680acagagaact cctcatggaa tttgaagggg cgcacgccac aaaacagtcc gttgttgctc 1740ttgggtcaca ggaaggaggc ctccatcatg cgttggcagg agccatcgtg gtggagtact 1800caagctcagt gatgttaaca tcaggccacc tgaaatgtag gctgaaaatg gacaaactgg 1860ctctgaaagg cacaacctat ggcatgtgta cagaaaaatt ctcgttcgcg aaaaatccgg 1920tggacactgg tcacggaaca gttgtcattg aactctccta ctctgggagt gatggcccct 1980gcaaaattcc gattgtttcc gttgcgagcc tcaatgacat gacccccgtt gggcggctgg 2040tgacagtgaa ccccttcgtc gcgacttcca gtgccaactc aaaggtgctg gtcgagatgg 2100aacccccctt cggagactcc tacatcgtag ttggaagggg agacaagcag atcaaccacc 2160attggcacaa agctggaagc acgctgggca aggccttttc aacaactttg aagggagctc 2220aaagactggc agcgttgggc gacacagcct gggactttgg ctctattgga ggggtcttca 2280actccatagg aagagccgtt caccaagtgt ttggtgatgc cttcagaaca ctctttgggg 2340gaatgtcttg gatcacacaa gggctaatgg gtgccctact gctctggatg ggcgtcaacg 2400cacgagaccg atcaattgct ttggccttct tagccacagg aggtgtgctc gtgttcttag 2460cgaccaatgt gcatgctgac actggatgtg ccattgacat cacaagaaaa gagatgagat 2520gtggaagtgg catcttcgtg cacaacgacg tggaagcctg ggtggatagg tataaatatt 2580tgccagaaac gcccagatcc ctagcgaaga tcgtccacaa agcgcacaag gaaggcgtgt 2640gcggagtcag atctgtcact agactggagc accaaatgtg ggaagccgta agggacgaat 2700tgaacgtcct gctcaaagag aatgcagtgg acctcagtgt ggttgtgaac aagcccgtgg 2760gaagatatcg ctcagcccct aaacgcctat ccatgacgca agagaagttt gaaatgggct 2820ggaaagcatg gggaaaaagc atcctctttg ccccggaatt ggctaactcc acatttgtcg 2880tagatggacc tgagacaaag gaatgccctg atgagcacag agcttggaac agcatgcaaa 2940tcgaagactt cggctttggc atcacatcaa cccgtgtgtg gctgaaaatt agagaggaga 3000gcactgacga gtgtgatgga gcgatcatag gcacggctgt caaaggacat gtggcagtcc 3060atagtgactt gtcgtactgg attgagagtc gctacaacga cacatggaaa cttgagaggg 3120cagtctttgg agaggtcaaa tcttgcactt ggccagagac acacaccctt tggggagatg 3180atgttgagga aagtgaactc atcattccgc acaccatagc cggaccaaaa agcaagcaca 3240atcggaggga agggtataag acacaaaacc agggaccttg ggatgagaat ggcatagtct 3300tggactttga ttattgccca gggacaaaag tcaccattac agaggattgt agcaagagag 3360gcccttcggt cagaaccact actgacagtg gaaagttgat cactgactgg tgctgtcgca 3420gttgctccct tccgccccta cgattccgga cagaaaatgg ctgctggtac ggaatggaaa 3480tcagacctgt tatgcatgat gaaacaacac tcgtcagatc acaggttcat gctttcaaag 3540gtgaaatggt tgaccctttt cagctgggcc ttctggtgat gtttctggcc acccaggaag 3600tccttcgcaa gaggtggacg gccagattga ccattcctgc ggttttgggg gtcctacttg 3660tgctgatgct tgggggtatc acttacactg atttggcgag gtatgtggtg ctagtcgctg 3720ctgctttcgc agaggccaac agtggaggag acgtcctgca ccttgctttg attgctgttt 3780ttaagatcca accagcattt ttagtgatga acatgcttag cacgagatgg acgaaccaag 3840aaaacgtggt tctggtccta ggggctgcct ttttccaatt ggcctcagta gatctgcaaa 3900taggagtcca cggaatcctg aatgccgccg ctatagcatg gatgattgtc cgagcgatca 3960ccttccccac aacctcctcc gtcaccatgc cagtcttagc gcttctaact ccggggatga 4020gggctctata cctagacact tacagaatca tcctcctcgt catagggatt tgctccctgc 4080tgcacgagag gaaaaagacc atggcgaaaa agaaaggagc tgtactcttg ggcttagcgc 4140tcacatccac tggatggttc tcgcccacca ctatagctgc cggactaatg gtctgcaacc 4200caaacaagaa gagagggtgg ccagctactg agtttttgtc ggcagttgga ttgatgtttg 4260ccatcgtagg tggtttggcc gagttggata ttgaatccat gtcaataccc ttcatgctgg 4320caggtctcat ggcagtgtcc tacgtggtgt caggaaaagc aacagatatg tggcttgaac 4380gggccgccga catcagctgg gatatgggtg ctgcaatcac aggaagcagt cggaggctgg 4440atgtgaaact ggatgatgac ggagattttc acttcattga tgatcccggt gttccatgga 4500aggtctgggt cctgcgcatg tcttgcattg gcttagccgc cctcacgcct tgggccatcg 4560ttcccgccgc tttcggttat tggctcactt taaaaacaac aaaaagaggg ggcgtgtttt 4620gggacacgcc atccccaaaa ccttgctcaa aaggagacac cactacagga gtctaccgaa 4680ttatggctag agggattctt ggcacttacc aggccggcgt cggagtcatg tacgagaatg 4740ttttccacac actatggcac acaactagag gagcagccat tgtgagtgga gaaggaaaat 4800tgacgccata ctggggtagt gtgaaagaag accgcatagc ttacggaggc ccatggaggt 4860ttgaccgaaa atggaatgga acagatgacg tgcaagtgat cgtggtagaa ccggggaagg 4920gcgcagtaaa catccagaca aaaccaggag tgtttcggac tcccttcggg gaggttgggg 4980ctgttagtct ggattacccg cgaggaacat ccggctcacc cattctggat tccaatggag 5040acattatagg cctatacggc aatggagttg agcttggcga tggctcatac gtcagcgcca 5100tcgtgcaggg tgaccgtcag gaggaaccag tcccagaagc ttacacccca aacatgttga 5160gaaagagaca gatgactgtg ctagatttgc accctggttc agggaaaacc aggaaaattc 5220tgccacaaat aattaaggac gctatccagc agcgcctaag aacagctgtg ttggcaccga 5280cgcgggtggt agcagcagaa atggcagaag ttttgagagg gctcccagta cgatatcaaa 5340cttcagcagt gcagagagag caccaaggga atgaaatagt ggatgtgatg tgccacgcca 5400ctctgaccca tagactgatg tcaccgaaca gagtgcccaa ctacaaccta tttgtcatgg 5460atgaagctca tttcaccgac ccagccagta tagccgcacg aggatacatt gctaccaagg 5520tggaattagg ggaggcagca gccatcttta tgacagcgac cccgcctgga accacggatc 5580cttttcctga ctcaaatgcc ccaatccatg atttgcaaga tgagatacca gacagggcat 5640ggagcagtgg atacgaatgg atcacagaat atgcgggtaa aaccgtgtgg tttgtggcga 5700gcgtaaaaat ggggaatgag attgcaatgt gcctccaaag agcggggaaa aaggtcatcc 5760aactcaaccg caagtcctat gacacagaat acccaaaatg taagaatgga gactgggatt 5820ttgtcattac caccgacatc tctgaaatgg gggccaactt cggtgcgagc agggtcatcg 5880actgtagaaa gagcgtgaaa cccaccatct tagaagaggg agaaggcaga gtcatcctcg 5940gaaacccatc tcccataacc agtgcaagcg cagctcaacg gaggggcaga gtaggcagaa 6000accccaatca agttggagat gaataccact atgggggggc taccagtgaa gatgacagta 6060acctagccca ttggacagag gcaaagatca tgttagacaa catacacatg cccaatggac 6120tggtggccca gctctatgga ccagagaggg aaaaggcttt cacaatggat ggcgaatacc 6180gtctcagagg tgaagaaaag aaaaacttct tagagctgct taggacggct gacctcccgg 6240tgtggctggc ctacaaggtg gcgtccaatg gcattcagta caccgacaga aagtggtgtt 6300ttgatgggcc gcgtacgaat gccatactgg aggacaacac cgaggtagag atagtcaccc 6360ggatgggtga gaggaaaatc ctcaagccga gatggcttga tgcaagagtt tatgcagatc 6420accaggccct caagtggttc aaagactttg cagcagggaa gagatcagcc gttagcttca 6480tagaggtgct cggtcgcatg cctgagcatt tcatgggaaa gacgcgggaa gctttagaca 6540ccatgtactt ggttgcaacg gctgagaaag gtgggaaagc acaccgaatg gctctcgaag 6600agctgccaga tgcactggaa accatcacac ttattgtcgc cattactgtg atgacaggag 6660gattcttcct actaatgatg cagcgaaagg gtatagggaa gatgggtctt ggagctctag 6720tgctcacact agctaccttc ttcctgtggg cggcagaggt tcctggaacc aaaatagcag 6780ggaccctgct gatcgccctg ctgctgatgg tggttctcat cccagaaccg gaaaaacaga 6840ggtcacagac agataaccaa ctggcggtgt ttctcatctg tgtcttgacc gtggttggag 6900tggtggcagc aaacgagtac gggatgctag aaaaaaccaa agcggatctc aagagcatgt 6960ttggcggaaa gacgcaggca tcaggactga ctggattgcc aagcatggca ctggacctgc 7020gtccagccac agcctgggca ctgtatgggg ggagcacagt cgtgctaacc cctcttctga 7080agcacctgat cacgtcggaa tacgtcacca catcgctagc ttcaattaac tcacaagctg 7140gctcattatt cgtcttgcca cgaggcgtgc cttttaccga cctagacttg actgttggcc 7200tcgtcttcct tggctgttgg ggtcaagtca ccctcacaac gtttctgaca gccatggttc 7260tggcgacact tcactatggg tacatgctcc ctggatggca agcagaagca ctcagggctg 7320cccagagaag gacagcggct ggaataatga agaatgccgt tgttgacgga atggtcgcca 7380ctgatgtgcc tgaactggaa aggactactc ctctgatgca aaagaaagtc ggacaggtgc 7440tcctcatagg ggtaagcgtg gcagcgttcc tcgtcaaccc taatgtcacc actgtgagag 7500aagcaggggt gttggtgacg gcggctacgc ttactttgtg ggacaatgga gccagtgccg 7560tttggaattc caccacagcc acgggactct gccatgtcat gcgaggtagc tacctggctg 7620gaggctccat tgcttggact ctcatcaaga acgctgataa gccctccttg aaaaggggaa 7680ggcctggggg caggacgcta ggggagcagt ggaaggaaaa actaaatgcc atgagtagag 7740aagagttttt taaataccgg agagagggca taatcgaggt ggaccgcact gaagcacgca 7800gggccagaag tgaaaataac atagtgggag gacatccggt ttcgcgaggc tcagcaaaac 7860tccgttggct tgtggagaaa ggatttgtct cgccaatagg aaaagtcatt gatctagggt 7920gtgggcgtgg aggatggagc tactacgcag caaccctgaa gaaggtccag gaagtcagag 7980gatacacgaa aggtggggcg ggacatgaag aaccgatgct catgcagagc tacggctgga 8040acctggtctc cctgaagagt ggagtggacg tgttttacaa accttcagag cccagtgata 8100ccctgttctg tgacataggg gaatcctccc caagtccaga agtagaagaa caacgcacac 8160tacgcgtcct agagatgaca tctgactggt tgcaccgagg acctagagag ttctgcatta 8220aagttctctg cccttacatg cccaaggtta tagaaaaaat tgaagttctg cagcgccgct 8280tcggaggtgg gctagtgcgt ctccccctgt cccgaaactc caatcacgag atgtattggg 8340ttagtggagc cgctggcaat gtggtgcacg ctgtgaacat gaccagccag gtattactgg 8400ggcgaatgga tcgcacagtg tggagagggc caaagtatga ggaagatgtc aacctaggga 8460gcggaacaag agccgtggga aagggagaag tccatagcaa tcaggagaaa atcaagaaga 8520gaatccagaa gcttaaagaa gaattcgcca caacgtggca caaagaccct gagcatccat 8580accgcacttg gacataccac ggaagctatg aagtgaaggc tactggctca gccagctctc 8640tcgtcaacgg agtggtgaag ctcatgagca aaccttggga cgccattgcc aacgtcacca 8700ccatggccat gactgacacc accccttttg gacagcaaag agttttcaag gagaaagttg 8760acacgaaggc tcctgagcca ccagctggag ccaaggaagt gctcaacgag accaccaact 8820ggctgtgggc ctacttgtca cgggaaaaaa gaccccgctt gtgcaccaag gaagaattca 8880ttaagaaagt taacagcaac gcggctcttg gagcagtgtt cgctgaacag aatcaatgga 8940gcacggcgcg tgaggctgtg gatgacccgc ggttttggga gatggttgat gaagagaggg 9000aaaaccatct gcgaggagag tgtcacacat gtatctacaa catgatggga aaaagagaga 9060agaagcctgg agagtttgga aaagctaaag gaagcagggc catttggttc atgtggcttg 9120gagcacggta tctagagttt gaagctttgg ggttcctgaa tgaagaccat tggctgagcc 9180gagagaattc aggaggtgga gtggaaggct caggcgtcca aaagctggga tacatcctcc 9240gtgacatagc aggaaagcaa ggagggaaaa tgtacgctga tgataccgcc gggtgggaca 9300ctagaattac cagaactgat ttagaaaatg aagctaaggt actggagctc ctagacggtg 9360aacaccgcat gctcgcccga gccataattg aactgactta caggcacaaa gtggtcaagg 9420tcatgagacc tgcagcagaa ggaaagaccg tgatggacgt gatatcaaga gaagatcaaa 9480gggggagtgg acaggtggtc acttatgctc ttaacacttt cacgaacatc gctgtccagc 9540tcgtcaggct gatggaggct gagggggtca ttggaccaca acacttggaa catctaccta 9600ggaaaaacaa gatagctgtc aggacctggc tctttgagaa tggagaggag agagtgacca 9660ggatggcgat cagcggagac gactgtgccg tcaaaccgct ggacgacaga ttcgccacag 9720ccctccactt cctcaacgca atgtcaaagg tcagaaaaga catccaggaa tggaagcctt 9780cgcatggctg gcacgattgg cagcaagttc ccttctgttc taaccatttt caggagattg 9840tgatgaaaga tggaaggagt atagttgtcc cgtgcagagg acaggatgag ctgataggca 9900gggctcgcat ctctccagga gctggatgga atgtgaagga cacagcttgc ctgcccaaag 9960catatgcaca aatgtgggta ctcctatact tccaccgcag ggacttgcgt ctcatggcaa 10020atgcgatttg ctcagcagtg ccagtagatt gggtgcccac aggcaggaca tcctggtcaa 10080tacactcgaa aggagagtgg atgaccacgg aagacatgct gcaggtctgg aacagagttt 10140ggattgaaga aaatgaatgg atgatggaca agactccaat cacaagctgg acagacgttc 10200cgtatgtggg aaagcgcgag gacatctggt gtggcagcct

catcggaacg cgatccagag 10260caacctgggc tgagaacatc tatgcggcga taaaccaggt tagagctgtc attgggaaag 10320aaaattatgt tgactacatg acctcactca ggagatacga agacgtcttg atccaggaag 10380acagggtcat ctagtgtgat ttaaggtaga aaagtagact atgtaaacaa tgtaaatgag 10440aaaatgcatg catatggagt caggccagca aaagctgcca ccggatactg ggtagacggt 10500gctgcctgcg tctcagtccc aggaggactg ggttaacaaa tctgacaaca gaaagtgaga 10560aagccctcag aactgtctcg gaagtaggtc cctgctcact ggaagttgaa agaccaacgt 10620caggccacaa atttgtgcca ctccgctagg gagtgcggcc tgcgcagccc caggaggact 10680gggttaccaa agccgttgag cccccacggc ccaagcctcg tctaggatgc aatagacgag 10740gtgtaaggac tagaggttag aggagacccc gtggaaacaa caacatgcgg cccaagcccc 10800ctcgaagctg tagaggaggt ggaaggacta gaggttagag gagaccccgc atttgcatca 10860aacagcatat tgacacctgg gaatagactg ggagatcttc tgctctatct caacatcagc 10920tactaggcac agagcgccga agtatgtacg tggtggtgag gaagaacaca ggatct 109767610838DNAYellow fever virus 76gtgctaattg aggtgcattg gtctgcaaat cgagttgcta ggcaataaac acatttggat 60taattttaat cgttcgttga gcgattagca gagaactgac cagaacatgt ctggtcgtaa 120agctcaggga aaaaccctgg gcgtcaatat ggtacgacga ggagttcgct ccttgtcaaa 180caaaataaaa caaaaaacaa aacaaattgg aaacagacct ggaccttcaa gaggtgttca 240aggatttatc tttttctttt tgttcaacat tttgactgga aaaaagatca cagcccacct 300aaagaggttg tggaaaatgc tggacccaag acaaggcttg gctgttctaa ggaaagtcaa 360gagagtggtg gccagtttga tgagaggatt gtcctcaagg aaacgccgtt cccatgatgt 420tctgactgtg caattcctaa ttttgggaat gctgttgatg acgggtggag tgaccttggt 480gcggaaaaac agatggttgc tcctaaatgt gacatctgag gacctcggga aaacattctc 540tgtgggcaca ggcaactgca caacaaacat tttggaagcc aagtactggt gcccagactc 600aatggaatac aactgtccca atctcagtcc aagagaggag ccagatgaca ttgattgctg 660gtgctatggg gtggaaaacg ttagagtcgc atatggtaag tgtgactcag caggcaggtc 720taggaggtca agaagggcca ttgacttgcc tacgcatgaa aaccatggtt tgaagacccg 780gcaagaaaaa tggatgactg gaagaatggg tgaaaggcaa ctccaaaaga ttgagagatg 840gttcgtgagg aacccctttt ttgcagtgac ggctctgacc attgcctacc ttgtgggaag 900caacatgacg caacgagtcg tgattgccct actggtcttg gctgttggtc cggcctactc 960agctcactgc attggaatta ctgacaggga tttcattgag ggggtgcatg gaggaacttg 1020ggtttcagct accctggagc aagacaagtg tgtcactgtt atggcccctg acaagccttc 1080attggacatc tcactagaga cagtagccat tgatagacct gctgaggtga ggaaagtgtg 1140ttacaatgca gttctcactc atgtgaagat taatgacaag tgccccagca ctggagaggc 1200ccacctagct gaagagaacg aaggggacaa tgcgtgcaag cgcacttatt ctgatagagg 1260ctggggcaat ggctgtggcc tatttgggaa agggagcatt gtggcatgcg ccaaattcac 1320ttgtgccaaa tccatgagtt tgtttgaggt tgatcagacc aaaattcagt atgtcatcag 1380agcacaattg catgtagggg ccaagcagga aaattggact accgacatta agactctcaa 1440gtttgatgcc ctgtcaggct cccaggaagt cgagttcatt gggtatggaa aagctacact 1500ggaatgccag gtgcaaactg cggtggactt tggtaacagt tacatcgctg agatggaaac 1560agagagctgg atagtggaca gacagtgggc ccaggacttg accctgccat ggcagagtgg 1620aagtggcggg gtgtggagag agatgcatca tcttgtcgaa tttgaacctc cgcatgccgc 1680cactatcaga gtactggccc tgggaaacca ggaaggctcc ttgaaaacag ctcttactgg 1740cgcaatgagg gttacaaagg acacaaatga caacaacctt tacaaactac atggtggaca 1800tgtttcttgc agagtgaaat tgtcagcttt gacactcaag gggacatcct acaaaatatg 1860cactgacaaa atgttttttg tcaagaaccc aactgacact ggccatggca ctgttgtgat 1920gcaggtgaaa gtgtcaaaag gagccccctg caggattcca gtgatagtag ctgatgatct 1980tacagcggca atcaataaag gcattttggt tacagttaac cccatcgcct caaccaatga 2040tgatgaagtg ctgattgagg tgaacccacc ttttggagac agctacatta tcgttgggag 2100aggagattca cgtctcactt accagtggca caaagaggga agctcaatag gaaagttgtt 2160cactcagacc atgaaaggcg tggaacgcct ggccgtcatg ggagacaccg cctgggattt 2220cagctccgct ggagggttct tcacttcggt tgggaaagga attcatacgg tgtttggctc 2280tgcctttcag gggctatttg gcggcttgaa ctggataaca aaggtcatca tgggggcggt 2340acttatatgg gttggcatca acacaagaaa catgacaatg tccatgagca tgatcttggt 2400aggagtgatc atgatgtttt tgtctctagg agttggggcg gatcaaggat gcgccatcaa 2460ctttggcaag agagagctca agtgcggaga tggtatcttc atatttagag actctgatga 2520ctggctgaac aagtactcat actatccaga agatcctgtg aagcttgcat caatagtgaa 2580agcctctttt gaagaaggga agtgtggcct aaattcagtt gactcccttg agcatgagat 2640gtggagaagc agggcagatg agatcaatgc catttttgag gaaaacgagg tggacatttc 2700tgttgtcgtg caggatccaa agaatgttta ccagagagga actcatccat tttccagaat 2760tcgggatggt ctgcagtatg gttggaagac ttggggtaag aaccttgtgt tctccccagg 2820gaggaagaat ggaagcttca tcatagatgg aaagtccagg aaagaatgcc cgttttcaaa 2880ccgggtctgg aattctttcc agatagagga gtttgggacg ggagtgttca ccacacgcgt 2940gtacatggac gcagtctttg aatacaccat agactgcgat ggatctatct tgggtgcagc 3000ggtgaacgga aaaaagagtg cccatggctc tccaacattt tggatgggaa gtcatgaagt 3060aaatgggaca tggatgatcc acaccttgga ggcattagat tacaaggagt gtgagtggcc 3120actgacacat acgattggaa catcagttga agagagtgaa atgttcatgc cgagatcaat 3180cggaggccca gttagctctc acaatcatat ccctggatac aaggttcaga cgaacggacc 3240ttggatgcag gtaccactag aagtgaagag agaagcttgc ccagggacta gcgtgatcat 3300tgatggcaac tgtgatggac ggggaaaatc aaccagatcc accacggata gcgggaaagt 3360tattcctgaa tggtgttgcc gctcctgcac aatgccgcct gtgagcttcc atggtagtga 3420tgggtgttgg tatcccatgg aaattaggcc aaggaaaacg catgaaagcc atctggtgcg 3480ctcctgggtt acagctggag aaatacatgc tgtccctttt ggtttggtga gcatgatgat 3540agcaatggaa gtggtcctaa ggaaaagaca gggaccaaag caaatgttgg ttggaggagt 3600agtgctcttg ggagcaatgc tggtcgggca agtaactctc cttgatttgc tgaaactcac 3660agtggctgtg ggattgcatt tccatgagat gaacaatgga ggagacgcca tgtatatggc 3720gttgattgct gccttttcaa tcagaccagg gctgctcatc ggctttgggc tcaggaccct 3780atggagccct cgggaacgcc ttgtgctgac cctaggagca gccatggtgg agattgcctt 3840gggtggcgtg atgggcggcc tgtggaagta tctaaatgca gtttctctct gcatcctgac 3900aataaatgct gttgcttcta ggaaagcatc aaataccatc ttgcccctca tggctctgtt 3960gacacctgtc actatggctg aggtgagact tgccgcaatg ttcttttgtg ccgtggttat 4020cataggggtc cttcaccaga atttcaagga cacctccatg cagaagacta tacctctggt 4080ggccctcaca ctcacatctt acctgggctt gacacaacct tttttgggcc tgtgtgcatt 4140tctggcaacc cgcatatttg ggcgaaggag tatcccagtg aatgaggcac tcgcagcagc 4200tggtctagtg ggagtgctgg caggactggc ttttcaggag atggagaact tccttggtcc 4260gattgcagtt ggaggactcc tgatgatgct ggttagcgtg gctgggaggg tggatgggct 4320agagctcaag aagcttggtg aagtttcatg ggaagaggag gcggagatca gcgggagttc 4380cgcccgctat gatgtggcac tcagtgaaca aggggagttc aagctgcttt ctgaagagaa 4440agtgccatgg gaccaggttg tgatgacctc gctggccttg gttggggctg ccctccatcc 4500atttgctctt ctgctggtcc ttgctgggtg gctgtttcat gtcaggggag ctaggagaag 4560tggggatgtc ttgtgggata ttcccactcc taagatcatc gaggaatgtg aacatctgga 4620ggatgggatt tatggcatat tccagtcaac cttcttgggg gcctcccagc gaggagtggg 4680agtggcacag ggaggggtgt tccacacaat gtggcatgtc acaagaggag ctttccttgt 4740caggaatggc aagaagttga ttccatcttg ggcttcagta aaggaagacc ttgtcgccta 4800tggtggctca tggaagttgg aaggcagatg ggatggagag gaagaggtcc agttgatcgc 4860ggctgttcca ggaaagaacg tggtcaacgt ccagacaaaa ccgagcttgt tcaaagtgag 4920gaatggggga gaaatcgggg ctgtcgctct tgactatccg agtggcactt caggatctcc 4980tattgttaac aggaacggag aggtgattgg gctgtacggc aatggcatcc ttgtcggtga 5040caactccttc gtgtccgcca tatcccagac tgaggtgaag gaagaaggaa aggaggagct 5100ccaagagatc ccgacaatgc taaagaaagg aatgacaact gtccttgatt ttcatcctgg 5160agctgggaag acaagacgtt tcctcccaca gatcttggcc gagtgcgcac ggagacgctt 5220gcgcactctt gtgttggccc ccaccagggt tgttctttct gaaatgaagg aggcttttca 5280cggcctggac gtgaaattcc acacacaggc tttttccgct cacggcagcg ggagagaagt 5340cattgatgct atgtgccatg ccaccctaac ttacaggatg ttggaaccaa ctagggttgt 5400taactgggaa gtgatcatta tggatgaagc ccattttttg gatccagcta gcatagccgc 5460tagaggttgg gcagcgcaca gagctagggc aaatgaaagt gcaacaatct tgatgacagc 5520cacaccgcct gggactagtg atgaatttcc acattcaaat ggtgaaatag aagatgttca 5580aacggacata cccagtgagc cctggaacac agggcatgac tggatcctgg ctgacaaaag 5640gcccacggca tggttccttc catccatcag agctgcaaat gtcatggctg cctctttgcg 5700taaggctgga aagagtgtgg tggtcctgaa caggaaaacc tttgagagag aataccccac 5760gataaagcag aagaaacctg actttatatt ggccactgac atagctgaaa tgggagccaa 5820cctttgcgtg gagcgagtgc tggattgcag gacggctttt aagcctgtgc ttgtggatga 5880agggaggaag gtggcaataa aagggccact tcgtatctcc gcatcctctg ctgctcaaag 5940gagggggcgc attgggagaa atcccaacag agatggagac tcatactact attctgagcc 6000tacaagtgaa aataatgccc accacgtctg ctggttggag gcctcaatgc tcttggacaa 6060catggaggtg aggggtggaa tggtcgcccc actctatggc gttgaaggaa ctaaaacacc 6120agtttcccct ggtgaaatga gactgaggga tgaccagagg aaagtcttca gagaactagt 6180gaggaattgt gacctgcccg tttggctttc gtggcaagtg gccaaggctg gtttgaagac 6240gaatgatcgt aagtggtgtt ttgaaggccc tgaggaacat gagatcttga atgacagcgg 6300tgaaacagtg aagtgcaggg ctcctggagg agcaaagaag cctctgcgcc caaggtggtg 6360tgatgaaagg gtgtcatctg accagagtgc gctgtctgaa tttattaagt ttgctgaagg 6420taggagggga gctgctgaag tgctagttgt gctgagtgaa ctccctgatt tcctggctaa 6480aaaaggtgga gaggcaatgg ataccatcag tgtgtttctc cactctgagg aaggctctag 6540ggcttaccgc aatgcactat caatgatgcc tgaggcaatg acaatagtca tgctgtttat 6600actggctgga ctactgacat cgggaatggt catctttttc atgtctccca aaggcatcag 6660tagaatgtct atggcgatgg gcacaatggc cggctgtgga tatctcatgt tccttggagg 6720cgtcaaaccc actcacatct cctatatcat gctcatattc tttgtcctga tggtggttgt 6780gatccccgag ccagggcaac aaaggtccat ccaagacaac caagtggcat acctcattat 6840tggcatcctg acgctggttt cagcggtggc agccaacgag ctaggcatgc tggagaaaac 6900caaagaggac ctctttggga agaagaactt aattccatct agtgcttcac cctggagttg 6960gccggatctt gacctgaagc caggagctgc ctggacagtg tacgttggca ttgttacaat 7020gctctctcca atgttgcacc actggatcaa agtcgaatat ggcaacctgt ctctgtctgg 7080aatagcccag tcagcctcag tcctttcttt catggacaag gggataccat tcatgaagat 7140gaatatctcg gtcataatgc tgctggtcag tggctggaat tcaataacag tgatgcctct 7200gctctgtggc atagggtgcg ccatgctcca ctggtctctc attttacctg gaatcaaagc 7260gcagcagtca aagcttgcac agagaagggt gttccatggc gttgccaaga accctgtggt 7320tgatgggaat ccaacagttg acattgagga agctcctgaa atgcctgccc tttatgagaa 7380gaaactggct ctatatctcc ttcttgctct cagcctagct tctgttgcca tgtgcagaac 7440gcccttttca ttggctgaag gcattgtcct agcatcagct gccctagggc cgctcataga 7500gggaaacacc agccttcttt ggaatggacc catggctgtc tccatgacag gagtcatgag 7560ggggaatcac tatgcttttg tgggagtcat gtacaatcta tggaagatga aaactggacg 7620ccgggggagc gcgaatggaa aaactttggg tgaagtctgg aagagggaac tgaatctgtt 7680ggacaagcga cagtttgagt tgtataaaag gaccgacatt gtggaggtgg atcgtgatac 7740ggcacgcagg catttggccg aagggaaggt ggacaccggg gtggcggtct ccagggggac 7800cgcaaagtta aggtggttcc atgagcgtgg ctatgtcaag ctggaaggta gggtgattga 7860cctggggtgt ggccgcggag gctggtgtta ctacgctgct gcgcaaaagg aagtgagtgg 7920ggtcaaagga tttactcttg gaagagacgg ccatgagaaa cccatgaatg tgcaaagtct 7980gggatggaac atcatcacct tcaaggacaa aactgatatc caccgcctag aaccagtgaa 8040atgtgacacc cttttgtgtg acattggaga gtcatcatcg tcatcggtca cagaggggga 8100aaggaccgtg agagttcttg atactgtaga aaaatggctg gcttgtgggg ttgacaactt 8160ctgtgtgaag gtgttagctc catacatgcc agatgttctc gagaaactgg aattgctcca 8220aaggaggttt ggcggaacag tgatcaggaa ccctctctcc aggaattcca ctcatgaaat 8280gtactacgtg tctggagccc gcagcaatgt cacatttact gtgaaccaaa catcccgcct 8340cctgatgagg agaatgaggc gtccaactgg aaaagtgacc ctggaggctg acgtcatcct 8400cccaattggg acacgcagtg ttgagacaga caagggaccc ctggacaaag aggccataga 8460agaaagggtt gagaggataa aatctgagta catgacctct tggttttatg acaatgacaa 8520cccctacagg acctggcact actgtggctc ctatgtcaca aaaacctcag gaagtgcggc 8580gagcatggta aatggtgtta ttaaaattct gacatatcca tgggacagga tagaggaggt 8640cacaagaatg gcaatgactg acacaacccc ttttggacag caaagagtgt ttaaagaaaa 8700agttgacacc agagcaaagg atccaccagc gggaactagg aagatcatga aagttgtcaa 8760caggtggctg ttccgccacc tggccagaga aaagaacccc agactgtgca caaaggaaga 8820atttattgca aaagtccgaa gtcatgcagc cattggagct tacctggaag aacaagaaca 8880gtggaagact gccaatgagg ctgtccaaga cccaaagttc tgggaactgg tggatgaaga 8940aaggaagctg caccaacaag gcaggtgtcg gacttgtgtg tacaacatga tggggaaaag 9000agagaagaag ctgtcagagt ttgggaaagc aaagggaagc cgtgccatat ggtatatgtg 9060gctgggagcg cggtatcttg agtttgaggc cctgggattc ctgaatgagg accattgggc 9120ttccagggaa aactcaggag gaggagtgga aggcattggc ttacaatacc taggatatgt 9180gatcagagac ctggctgcaa tggatggtgg tggattctac gcggatgaca ccgctggatg 9240ggacacgcgc atcacagagg cagaccttga tgatgaacag gagatcttga actacatgag 9300cccacatcac aaaaaactgg cacaagcagt gatggaaatg acatacaaga acaaagtggt 9360gaaagtgttg agaccagccc caggagggaa agcctacatg gatgtcataa gtcgacgaga 9420ccagagagga tccgggcagg tagtgactta tgctctgaac accatcacca acttgaaagt 9480ccaattgatc agaatggcag aagcagagat ggtgatacat caccaacatg ttcaagattg 9540tgatgaatca gttctgacca ggctggaggc atggctcact gagcacggat gtaacagact 9600gaagaggatg gcggtgagtg gagacgactg tgtggtccgg cccatcgatg acaggttcgg 9660cctggccctg tcccatctca acgccatgtc caaggttaga aaggacatat ctgaatggca 9720gccatcaaaa gggtggaatg attgggagaa tgtgcccttc tgttcccacc acttccatga 9780actacagctg aaggatggca ggaggattgt ggtgccttgc cgagaacagg acgagctcat 9840tgggagagga agggtgtctc caggaaacgg ctggatgatc aaggaaacag cttgcctcag 9900caaagcctat gccaacatgt ggtcactgat gtattttcac aaaagggaca tgaggctact 9960gtcattggct gtttcctcag ctgttcccac ctcatgggtt ccacaaggac gcacaacatg 10020gtcgattcat gggaaagggg agtggatgac cacggaagac atgcttgagg tgtggaacag 10080agtatggata accaacaacc cacacatgca ggacaagaca atggtgaaaa aatggagaga 10140tgtcccttat ctaaccaaga gacaagacaa gctgtgcgga tcactgattg gaatgaccaa 10200tagggccacc tgggcctccc acatccattt ggtcatccat cgtatccgaa cgctgattgg 10260acaggagaaa tacactgact acctaacagt catggacagg tattctgtgg atgctgacct 10320gcaactgggt gagcttatct gaaacaccat ctaacaggaa taaccgggat acaaaccacg 10380ggtggagaac cggactcccc acaacctgaa accgggatat aaaccacggc tggagaaccg 10440gactccgcac ttaaaatgaa acagaaaccg ggataaaaac tacggatgga gaaccggact 10500ccacacattg agacagaaga agttgtcagc ccagaacccc acacgagttt tgccactgct 10560aagctgtgag gcagtgcagg ctgggacagc cgacctccag gttgcgaaaa acctggtttc 10620tgggacctcc caccccagag taaaaagaac ggagcctccg ctaccaccct cccacgtggt 10680ggtagaaaga cggggtctag aggttagagg agaccctcca gggaacaaat agtgggacca 10740tattgacgcc agggaaagac cggagtggtt ctctgctttt cctccagagg tctgtgagca 10800cagtttgctc aagaataagc agacctttgg atgacaaa 108387711674DNAChikungunya virus 77gatggctgcg tgagacacac gtagcctacc agtttcttac tgctctactc tgcaaagcaa 60gagattaata acccatcatg gatcctgtgt acgtggacat agacgctgac agcgcctttt 120tgaaggccct gcaacgtgcg taccccatgt ttgaggtgga accaaggcag gtcacaccga 180atgaccatgc taatgctaga gcgttctcgc atctagctat aaaactaata gagcaggaaa 240ttgaccccga ctcaaccatc ctggatatcg gcagtgcgcc agcaaggagg atgatgtcgg 300acaggaagta ccactgcgtc tgcccgatgc gcagtgcgga agatcccgag agactcgcca 360attatgcgag aaagctagca tctgccgcag gaaaagtcct ggacagaaac atctctggaa 420agatcgggga cttacaagca gtaatggccg tgccagacac ggagacgcca acattctgct 480tacacacaga cgtctcatgt agacagagag cagacgtcgc tatataccaa gacgtctatg 540ctgtacacgc acccacgtcg ctataccacc aggcgattaa aggggtccga gtggcgtact 600gggttgggtt cgacacaacc ccgttcatgt acaatgccat ggcgggtgcc tacccctcat 660actcgacaaa ctgggcagat gagcaggtac tgaaggctaa gaacatagga ttatgttcaa 720cagacctgac ggaaggtaga cgaggcaagt tgtctattat gagagggaaa aagctaaaac 780cgtgcgaccg tgtgctgttc tcagtagggt caacgctcta cccggaaagc cgcaagctac 840ttaagagctg gcacctgcca tcggtgttcc atttaaaggg caaactcagc ttcacatgcc 900gctgtgatac agtggtttcg tgtgagggct acgtcgttaa gagaataacg atgagcccag 960gcctttatgg aaaaaccaca gggtatgcgg taacccacca cgcagacgga ttcctgatgt 1020gcaagactac cgacacggtt gacggcgaaa gaatgtcatt ctcggtgtgc acatacgtgc 1080cggcgaccat ttgtgatcaa atgaccggca tccttgctac agaagtcacg ccggaggatg 1140cacagaagct gttggtgggg ctgaaccaga gaatagtggt taacggcaga acgcaacgga 1200atacgaacac catgaaaaat tatctgcttc ccgtggtcgc ccaagccttc agtaagtggg 1260caaaggagtg ccggaaagac atggaagatg aaaaactcct gggggtcaga gaaagaacac 1320tgacctgctg ctgtctatgg gcattcaaga agcagaaaac acacacggtc tacaagaggc 1380ctgataccca gtcaattcag aaggttcagg ccgagtttga cagctttgtg gtaccgagtc 1440tgtggtcgtc cgggttgtca atccctttga ggactagaat caaatggttg ttaagcaagg 1500tgccaaaaac cgacctgatc ccatacagcg gagacgcccg agaagcccgg gacgcagaaa 1560aagaagcaga ggaagaacga gaagcagaac tgactcgcga agccctacca cctctacagg 1620cagcacagga agatgttcag gtcgaaatcg acgtggaaca gcttgaggac agagcgggcg 1680caggaataat agagactccg agaggagcta tcaaagttac tgcccaacca acagaccacg 1740tcgtgggaga gtacctggta ctctccccgc agaccgtact acgtagccag aagctcagtc 1800tgattcacgc tttggcggag caagtgaaga cgtgcacgca caacggacga gcagggaggt 1860atgcggtcga agcgtacgac ggccgagtcc tagtgccctc aggctatgca atctcgcctg 1920aagacttcca gagtctaagc gaaagcgcaa cgatggtgta taacgaaaga gagttcgtaa 1980acagaaagct acaccatatt gcgatgcacg gaccagccct gaacaccgac gaagagtcgt 2040atgagctggt gagggcagag aggacagaac acgagtacgt ctacgacgtg gatcagagaa 2100gatgctgtaa gaaggaagaa gccgcaggac tggtactggt gggcgacttg actaatccgc 2160cctaccacga attcgcatat gaagggctaa aaatccgccc tgcctgccca tacaaaattg 2220cagtcatagg agtcttcgga gtaccgggat ctggcaagtc agctattatc aagaacctag 2280ttaccaggca ggacctggtg actagcggaa agaaagaaaa ctgccaagaa atcaccaccg 2340acgtgatgag acagagaggt ctagagatat ctgcacgtac ggttgactcg ctgctcttga 2400atggatgcaa cagaccagtc gacgtgttgt acgtagacga ggcgtttgcg tgccactctg 2460gaacgctact tgctttgatc gccttggtga gaccaaggca gaaagttgta ctttgtggtg 2520acccgaagca gtgcggcttc ttcaatatga tgcagatgaa agtcaactat aatcacaaca 2580tctgcaccca agtgtaccac aaaagtatct ccaggcggtg tacactgcct gtgaccgcca 2640ttgtgtcatc gttgcattac gaaggcaaaa tgcgcactac gaatgagtac aacaagccga 2700ttgtagtgga cactacaggc tcaacaaaac ctgaccctgg agacctcgtg ttaacgtgct 2760tcagagggtg ggttaaacaa ctgcaaattg actatcgtgg atacgaggtc atgacagcag 2820ccgcatccca agggttaacc agaaaaggag tttacgcagt tagacaaaaa gttaatgaaa 2880acccgctcta tgcatcaacg tcagagcacg tcaacgtact cctaacgcgt acggaaggta 2940aactggtatg gaagacactt tccggcgacc cgtggataaa gacgctgcag aacccaccga 3000aaggaaactt caaagcaact attaaggagt gggaggtgga gcatgcatca ataatggcgg 3060gcatctgcag tcaccaaatg accttcgata cattccaaaa taaagccaac gtttgttggg 3120ctaagagctt ggtccctatc ctcgaaacag cggggataaa actaaatgat aggcagtggt 3180ctcagataat tcaagccttc aaagaagaca aagcatactc acctgaagta gccctgaatg 3240aaatatgtac gcgcatgtat ggggtggatc tagacagcgg gctattttct aaaccgttgg 3300tgtctgtgta ttacgcggat aaccactggg ataataggcc tggagggaaa atgttcggat 3360ttaaccccga ggcagcatcc attctagaaa

gaaagtatcc attcacaaaa gggaagtgga 3420acatcaacaa gcagatctgc gtgactacca ggaggataga agactttaac cctaccacca 3480acatcatacc ggccaacagg agactaccac actcattagt ggccgaacac cgcccagtaa 3540aaggggaaag aatggaatgg ctggttaaca agataaacgg ccaccacgtg ctcctggtca 3600gtggctataa ccttgcactg cctactaaga gagtcacttg ggtagcgccg ttaggtgtcc 3660gcggagcgga ctacacatac aacctagagt tgggtctgcc agcaacgctt ggtaggtatg 3720acctagtggt cataaacatc cacacacctt ttcgcataca ccattaccaa cagtgcgtcg 3780accacgcaat gaaactgcaa atgctcgggg gtgactcatt gagactgctc aaaccgggcg 3840gctctctatt gatcagagca tatggttacg cagatagaac cagtgaacga gtcatctgcg 3900tattgggacg caagtttaga tcgtctagag cgttgaaacc accatgtgtc accagcaaca 3960ctgagatgtt tttcctattc agcaactttg acaatggcag aaggaatttc acaactcatg 4020tcatgaacaa tcaactgaat gcagccttcg taggacaggt cacccgagca ggatgtgcac 4080cgtcgtaccg ggtaaaacgc atggacatcg cgaagaacga tgaagagtgc gtagtcaacg 4140ccgctaaccc tcgcgggtta ccgggtggcg gtgtttgcaa ggcagtatac aaaaaatggc 4200cggagtcctt taagaacagt gcaacaccag tgggaaccgc aaaaacagtt atgtgcggta 4260cgtatccagt aatccacgct gttggaccaa acttctctaa ttattcggag tctgaagggg 4320accgggaatt ggcagctgcc tatcgagaag tcgcaaagga agtaactagg ctgggagtaa 4380atagtgtagc tatacctctc ctctccacag gtgtatactc aggagggaaa gacaggctga 4440cccagtcact gaaccacctc tttacagcca tggactcgac ggatgcagac gtggtcatct 4500actgccgcga caaagaatgg gagaagaaaa tatctgaggc catacagatg cggacccaag 4560tagagctgct ggatgagcac atctccatag actgcgatat tgttcgcgtg caccctgaca 4620gcagcttggc aggcagaaaa ggatacagca ccacggaagg cgcactgtac tcatatctag 4680aagggacccg ttttcatcag acggctgtgg atatggcgga gatacatact atgtggccaa 4740agcaaacaga ggccaatgag caagtctgcc tatatgccct gggggaaagt attgaatcga 4800tcaggcagaa atgcccggtg gatgatgcag acgcatcatc tccccccaaa actgtcccgt 4860gcctttgccg ttacgctatg actccagaac gcgtcacccg gcttcgcatg aaccacgtca 4920caagcataat tgtgtgttct tcgtttcccc tcccaaagta caaaatagaa ggagtgcaaa 4980aagtcaaatg ctctaaggta atgctatttg accacaacgt gccatcgcgc gtaagtccaa 5040gggcttatag aggtgccgct gccggtaacc ttgcggccgt gtctgattgg gtaatgagca 5100ccgtacctgt cgcgccgccc agaagaaggc gagggagaaa cctgactgtg acatgtgacg 5160agagagaagg gaatataaca cccatggcta gcgtccgatt ctttagggca gagctgtgtc 5220cggtcgtaca agaaacagcg gagacgcgtg acacagcaat gtctcttcag gcaccaccga 5280gtaccgccac ggaaccgaat catccgccga tctccttcgg agcatcaagc gagacgttcc 5340ccattacatt tggggacttc aacgaaggag aaatcgaaag cttgtcttct gagctactaa 5400ctttcggaga cttcttacca ggagaagtgg atgacttgac agacagcgac tggtccacgt 5460gctcagacac ggacgacgag ttaagactag acagggcagg tgggtatata ttctcgtcgg 5520acaccggtcc aggtcattta caacagaagt cagtacgcca gtcagtgctg ccggtgaaca 5580ccctggagga agtccacgag gagaagtgtt acccacctaa gctggatgaa gcaaaggagc 5640aactattact taagaaactc caggagagtg catccatggc caacagaagc aggtatcagt 5700cgcgcaaagt agaaaacatg aaagcagcaa tcatccagag actaaagaga ggctgtagac 5760tatacttaat gtcagagacc ccaaaagtcc ctacttaccg gactacatat ccggcgcctg 5820tgtactcgcc tccgatcaac gtccgattgt ccaatcccga gtccgcagtg gcagcatgca 5880atgagttctt agctagaaac tatccaactg tctcatcata ccaaattacc gacgagtatg 5940atgcatatct agacatggtg gacgggtcgg agagttgcct ggaccgagcg acattcaatc 6000cgtcaaaact caggagctac ccgaaacagc acgcttacca cgcgccctcc atcagaagcg 6060ctgtaccgtc cccattccag aacacactac agaatgtact ggcagcagcc acgaaaagaa 6120actgcaacgt cacacagatg agggaattac ccactttgga ctcagcagta ttcaacgtgg 6180agtgtttcaa aaaattcgca tgcaaccaag aatactggga agaatttgct gccagcccta 6240ttaggataac aactgagaat ttagcaacct atgttactaa actaaaaggg ccaaaagcag 6300cagcgctatt cgcaaaaacc cataatctac tgccactaca ggaagtacca atggataggt 6360tcacagtaga tatgaaaagg gacgtaaagg tgactcctgg tacaaagcat acagaggaaa 6420gacctaaggt gcaggttata caggcggctg aacccttggc gacagcatac ctatgtggga 6480ttcacagaga gctggttagg aggctgaacg ccgtcctcct acccaatgta catacactat 6540ttgacatgtc tgccgaggat ttcgatgcca tcatagccgc acactttaag ccaggagaca 6600ctgttttgga aacggacata gcctcctttg ataagagcca agatgattca cttgcgctta 6660ctgctttgat gctgttagag gatttagggg tggatcactc cctgctggac ttgatagagg 6720ctgctttcgg agagatttcc agctgtcacc taccgacagg tacgcgcttc aagttcggcg 6780ccatgatgaa atcaggtatg ttcctaactc tgttcgtcaa cacattgtta aacatcacca 6840tcgccagccg agtgctggaa gatcgtctga caaaatccgc gtgcgcggcc ttcatcggcg 6900acgacaacat aatacatgga gtcgtctccg atgaattgat ggcagccaga tgtgccactt 6960ggatgaacat ggaagtgaag atcatagatg cagttgtatc cttgaaagcc ccttactttt 7020gtggagggtt tatactgcac gatactgtga caggaacagc ttgcagagtg gcagacccgc 7080taaaaaggct ttttaaactg ggcaaaccgc tagcggcagg tgacgaacaa gatgaagata 7140gaagacgagc gctggctgac gaagtgatca gatggcaacg aacagggcta attgatgagc 7200tggagaaagc ggtatactct aggtacgaag tgcagggtat atcagttgtg gtaatgtcca 7260tggccacctt tgcaagctcc agatccaact tcgagaagct cagaggaccc gtcataactt 7320tgtacggcgg tcctaaatag gtacgcacta cagctaccta ttttgcagaa gccgacagca 7380agtatctaaa cactaatcag ctacaatgga gttcatccca acccaaactt tttacaatag 7440gaggtaccag cctcgaccct ggactccgcg ccctactatc caagtcatca ggcccagacc 7500gcgccctcag aggcaagctg ggcaacttgc ccagctgatc tcagcagtta ataaactgac 7560aatgcgcgcg gtaccacaac agaagccacg caggaatcgg aagaataaga agcaaaagca 7620aaaacaacag gcgccacaaa acaacacaaa tcaaaagaag cagccaccta aaaagaaacc 7680ggctcaaaag aaaaagaagc cgggccgcag agagaggatg tgcatgaaaa tcgaaaatga 7740ttgtattttc gaagtcaagc acgaaggtaa ggtaacaggt tacgcgtgcc tggtggggga 7800caaagtaatg aaaccagcac acgtaaaggg gaccatcgat aacgcggacc tggccaaact 7860ggcctttaag cggtcatcta agtatgacct tgaatgcgcg cagatacccg tgcacatgaa 7920gtccgacgct tcgaagttca cccatgagaa accggagggg tactacaact ggcaccacgg 7980agcagtacag tactcaggag gccggttcac catccctaca ggtgctggca aaccagggga 8040cagcggcaga ccgatcttcg acaacaaggg acgcgtggtg gccatagtct taggaggagc 8100taatgaagga gcccgtacag ccctctcggt ggtgacctgg aataaagaca ttgtcactaa 8160aatcaccccc gagggggccg aagagtggag tcttgccatc ccagttatgt gcctgttggc 8220aaacaccacg ttcccctgct cccagccccc ttgcacgccc tgctgctacg aaaaggaacc 8280ggaggaaacc ctacgcatgc ttgaggacaa cgtcatgaga cctgggtact atcagctgct 8340acaagcatcc ttaacatgtt ctccccaccg ccagcgacgc agcaccaagg acaacttcaa 8400tgtctataaa gccacaagac catacttagc tcactgtccc gactgtggag aagggcactc 8460gtgccatagt cccgtagcac tagaacgcat cagaaatgaa gcgacagacg ggacgctgaa 8520aatccaggtc tccttgcaaa tcggaataaa gacggatgac agccacgatt ggaccaagct 8580gcgttatatg gacaaccaca tgccagcaga cgcagagagg gcggggctat ttgtaagaac 8640atcagcaccg tgtacgatta ctggaacaat gggacacttc atcctggccc gatgtccaaa 8700aggggaaact ctgacggtgg gattcactga cagtaggaag attagtcact catgtacgca 8760cccatttcac cacgaccctc ctgtgatagg tcgggaaaaa ttccattccc gaccgcagca 8820cggtaaagag ctaccttgca gcacgtacgt gcagagcacc gccgcaacta ccgaggagat 8880agaggtacac atgcccccag acacccctga tcgcacatta atgtcacaac agtccggcaa 8940cgtaaagatc acagtcaatg gccagacggt gcggtacaag tgtaattgcg gtggctcaaa 9000tgaaggacta acaactacag acaaagtgat taataactgc aaggttgatc aatgtcatgc 9060cgcggtcacc aatcacaaaa agtggcagta taactcccct ctggtcccgc gtaatgctga 9120acttggggac cgaaaaggaa aaattcacat cccgtttccg ctggcaaatg taacatgcag 9180ggtgcctaaa gcaaggaacc ccaccgtgac gtacgggaaa aaccaagtca tcatgctact 9240gtatcctgac cacccaacac tcctgtccta ccggaatatg ggagaagaac caaactatca 9300agaagagtgg gtgatgcata agaaggaagt cgtgctaacc gtgccgactg aagggctcga 9360ggtcacgtgg ggcaacaacg agccgtataa gtattggccg cagttatcta caaacggtac 9420agcccatggc cacccgcatg agataattct gtattattat gagctgtacc ccactatgac 9480tgtagtagtt gtgtcagtgg ccacgttcat actcctgtcg atggtgggta tggcagcggg 9540gatgtgcatg tgtgcacgac gcagatgcat cacaccgtat gaactgacac caggagctac 9600cgtccctttc ctgcttagcc taatatgctg catcagaaca gctaaagcgg ccacatacca 9660agaggctgcg atatacctgt ggaacgagca gcaacctttg ttttggctac aagcccttat 9720tccgctggca gccctgattg ttctatgcaa ctgtctgaga ctcttaccat gctgctgtaa 9780aacgttggct tttttagccg taatgagcgt cggtgcccac actgtgagcg cgtacgaaca 9840cgtaacagtg atcccgaaca cggtgggagt accgtataag actctagtca atagacctgg 9900ctacagcccc atggtattgg agatggaact actgtcagtc actttggagc caacactatc 9960gcttgattac atcacgtgcg agtacaaaac cgtcatcccg tctccgtacg tgaagtgctg 10020cggtacagca gagtgcaagg acaaaaacct acctgactac agctgtaagg tcttcaccgg 10080cgtctaccca tttatgtggg gcggcgccta ctgcttctgc gacgctgaaa acacgcagtt 10140gagcgaagca cacgtggaga agtccgaatc atgcaaaaca gaatttgcat cagcatacag 10200ggctcatacc gcatctgcat cagctaagct ccgcgtcctt taccaaggaa ataacatcac 10260tgtaactgcc tatgcaaacg gcgaccatgc cgtcacagtt aaggacgcca aattcattgt 10320ggggccaatg tcttcagcct ggacaccttt cgacaacaaa attgtggtgt acaaaggtga 10380cgtctataac atggactacc cgccctttgg cgcaggaaga ccaggacaat ttggcgatat 10440ccaaagtcgc acacctgaga gtaaagacgt ctatgctaat acacaactgg tactgcagag 10500accggctgtg ggtacggtac acgtgccata ctctcaggca ccatctggct ttaagtattg 10560gctaaaagaa cgcggggcgt cgctgcagca cacagcacca tttggctgcc aaatagcaac 10620aaacccggta agagcggtga actgcgccgt agggaacatg cccatctcca tcgacatacc 10680ggaagcggcc ttcactaggg tcgtcgacgc gccctcttta acggacatgt cgtgcgaggt 10740accagcctgc acccattcct cagactttgg gggcgtcgcc attattaaat atgcagccag 10800caagaaaggc aagtgtgcgg tgcattcgat gactaacgcc gtcactattc gggaagctga 10860gatagaagtt gaagggaatt ctcagctgca aatctctttc tcgacggcct tagccagcgc 10920cgaattccgc gtacaagtct gttctacaca agtacactgt gcagccgagt gccacccccc 10980gaaggaccac atagtcaact acccggcgtc acataccacc ctcggggtcc aggacatctc 11040cgctacggcg atgtcatggg tgcagaagat cacgggaggt gtgggactgg ttgttgctgt 11100tgccgcactg attctaatcg tggtgctatg cgtgtcgttc agcaggcact aacttgacaa 11160ttaagtatga aggtatatgt gtcccctaag agacacactg tacatagcaa ataatctata 11220gatcaaaggg ctacgcaacc cctgaatagt aacaaaatac aaaatcacta aaaattataa 11280aaacagaaaa atacataaat aggtatacgt gtcccctaag agacacattg tatgtaggtg 11340ataagtatag atcaaagggc cgaataaccc ctgaatagta acaaaatatg aaaatcaata 11400aaaatcataa aatagaaaaa ccataaacag aagtagttca aagggctata aaacccctga 11460atagtaacaa aacataaaat taataaaaat caaatgaata ccataattgg caaacggaag 11520agatgtaggt acttaagctt cctaaaagca gccgaactca ctttgagaag taggcatagc 11580ataccgaact cttccacgat tctccgaacc cacagggacg taggagatgt tattttgttt 11640ttaatatttc aaaaaaaaaa aaaaaaaaaa aaaa 116747810773DNAZika virus 78cagactgcga cagttcgagt ttgaagcgaa agctagcaac agtatcaaca ggttttattt 60tggatttgga aacgagagtt tctggtcatg aaaaacccaa aaaagaaatc cggaggattc 120cggattgtca atatgctaaa acgcggagta gcccgtgtga gcccctttgg gggcttgaag 180aggctgccag ccggacttct gctgggtcat gggcccatca ggatggtctt ggcgattcta 240gcctttttga gattcacggc aatcaagcca tcactgggtc tcatcaatag atggggttca 300gtggggaaaa aagaggctat ggaaataata aagaagttca agaaagatct ggctgccatg 360ctgagaataa tcaatgctag gaaggagaag aagagacgag gcgcagatac tagtgtcgga 420attgttggcc tcctgctgac cacagctatg gcagcggagg tcactagacg tgggagtgca 480tactatatgt acttggacag aaacgacgct ggggaggcca tatcttttcc aaccacattg 540gggatgaata agtgttatat acagatcatg gatcttggac acatgtgtga tgccaccatg 600agctatgaat gccctatgct ggatgagggg gtggaaccag atgacgtcga ttgttggtgc 660aacacgacgt caacttgggt tgtgtacgga acctgccatc acaaaaaagg tgaagcacgg 720agatctagaa gagctgtgac gctcccctcc cattccacta ggaagctgca aacgcggtcg 780caaacctggt tggaatcaag agaatacaca aagcacttga ttagagtcga aaattggata 840ttcaggaacc ctggcttcgc gttagcagca gctgccatcg cttggctttt gggaagctca 900acgagccaaa aagtcatata cttggtcatg atactgctga ttgccccggc atacagcatc 960aggtgcatag gagtcagcaa tagggacttt gtggaaggta tgtcaggtgg gacttgggtt 1020gatgttgtct tggaacatgg aggttgtgtc accgtaatgg cacaggacaa accgactgtc 1080gacatagagc tggttacaac aacagtcagc aacatggcgg aggtaagatc ctactgctat 1140gaggcatcaa tatcggacat ggcttcggac agccgctgcc caacacaagg tgaagcctac 1200cttgacaagc aatcagacac tcaatatgtc tgcaaaagaa cgttagtgga cagaggctgg 1260ggaaatggat gtggactttt tggcaaaggg agcctggtga catgcgctaa gtttgcatgc 1320tccaagaaaa tgaccgggaa gagcatccag ccagagaatc tggagtaccg gataatgctg 1380tcagttcatg gctcccagca cagtgggatg atcgttaatg acacaggaca tgaaactgat 1440gagaatagag cgaaggttga gataacgccc aattcaccaa gagccgaagc caccctgggg 1500ggttttggaa gcctaggact tgattgtgaa ccgaggacag gccttgactt ttcagatttg 1560tattacttga ctatgaataa caagcactgg ttggttcaca aggagtggtt ccacgacatt 1620ccattacctt ggcacgctgg ggcagacacc ggaactccac actggaacaa caaagaagca 1680ctggtagagt tcaaggacgc acatgccaaa aggcaaactg tcgtggttct agggagtcaa 1740gaaggagcag ttcacacggc ccttgctgga gctctggagg ctgagatgga tggtgcaaag 1800ggaaggctgt cctctggcca cttgaaatgt cgcctgaaaa tggataaact tagattgaag 1860ggcgtgtcat actccttgtg taccgcagcg ttcacattca ccaagatccc ggctgaaaca 1920ctgcacggga cagtcacagt ggaggtacag tacgcaggga cagatggacc ttgcaaggtt 1980ccagctcaga tggcggtgga catgcaaact ctgaccccag ttgggaggtt gataaccgct 2040aaccccgtaa tcactgaaag cactgagaac tctaagatga tgctggaact tgatccacca 2100tttggggact cttacattgt cataggagtc ggggagaaga agatcaccca ccactggcac 2160aggagtggca gcaccattgg aaaagcattt gaagccactg tgagaggtgc caagagaatg 2220gcagtcttgg gagacacagc ctgggacttt ggatcagttg gaggcgctct caactcattg 2280ggcaagggca tccatcaaat ttttggagca gctttcaaat cattgtttgg aggaatgtcc 2340tggttctcac aaattctcat tggaacgttg ctgatgtggt tgggtctgaa cacaaagaat 2400ggatctattt cccttatgtg cttggcctta gggggagtgt tgatcttctt atccacagct 2460gtctctgctg atgtggggtg ctcggtggac ttctcaaaga aggagacgag atgcggtaca 2520ggggtgttcg tctataacga cgttgaagcc tggagggaca ggtacaagta ccatcctgac 2580tccccccgta gattggcagc agcagtcaag caagcctggg aagatggtat ctgtgggatc 2640tcctctgttt caagaatgga aaacatcatg tggagatcag tagaagggga gctcaacgca 2700atcctggaag agaatggagt tcaactgacg gtcgttgtgg gatctgtaaa aaaccccatg 2760tggagaggtc cacagagatt gcccgtgcct gtgaacgagc tgccccacgg ctggaaggct 2820tgggggaaat cgtacttcgt cagagcagca aagacaaata acagctttgt cgtggatggt 2880gacacactga aggaatgccc actcaaacat agagcatgga acagctttct tgtggaggat 2940catgggttcg gggtatttca cactagtgtc tggctcaagg ttagagaaga ttattcatta 3000gagtgtgatc cagccgttat tggaacagct gttaagggaa aggaggctgt acacagtgat 3060ctaggctact ggattgagag tgagaagaat gacacatgga ggctgaagag ggcccatctg 3120atcgagatga aaacatgtga atggccaaag tcccacacat tgtggacaga tggaatagaa 3180gagagtgatc tgatcatacc caagtcttta gctgggccac tcagccatca caataccaga 3240gagggctaca ggacccaaat gaaagggcca tggcacagtg aagagcttga aattcggttt 3300gaggaatgcc caggcactaa ggtccacgtg gaggaaacat gtggaacaag aggaccatct 3360ctgagatcaa ccactgcaag cggaagggtg atcgaggaat ggtgctgcag ggagtgcaca 3420atgcccccac tgtcgttccg ggctaaagat ggctgttggt atggaatgga gataaggccc 3480aggaaagaac cagaaagtaa cttagtaagg tcaatggtga ctgcaggatc aactgatcac 3540atggatcact tctcccttgg agtgcttgtg attctgctca tggtgcagga agggctgaag 3600aagagaatga ccacaaagat catcataagc acatcgatgg cagtgctggt agctatgatc 3660ctgggaggat tttcaatgag tgacctggct aagcttgcaa ttttgatggg tgccaccttc 3720gcggaaatga acactggagg agatgtagct catctggcgc tgatagcggc attcaaagtc 3780agaccagcgt tgctggtatc tttcatcttc agagctaatt ggacaccccg tgaaagcatg 3840ctgctggcct tggcctcgtg tcttttgcaa actgcgatct ccgccttgga aggcgacctg 3900atggttctca tcaatggttt tgctttggcc tggttggcaa tacgagcgat ggttgttcca 3960cgcactgata acatcacctt ggcaatcctg gctgctctga caccactggc ccggggcaca 4020ctgcttgtgg cgtggagagc aggccttgct acttgcgggg ggtttatgct cctctctctg 4080aagggaaaag gcagtgtgaa gaagaactta ccatttgtca tggccctggg actaaccgct 4140gtgaggctgg tcgaccccat caacgtggtg ggactgctgt tgctcacaag gagtgggaag 4200cggagctggc cccctagcga agtactcaca gctgttggcc tgatatgcgc attggctgga 4260gggttcgcca aggcagatat agagatggct gggcccatgg ccgcggtcgg tctgctaatt 4320gtcagttacg tggtctcagg aaagagtgtg gacatgtaca ttgaaagagc aggtgacatc 4380acatgggaaa aagatgcgga agtcactgga aacagtcccc ggctcgatgt ggcgctagat 4440gagagtggtg atttctccct ggtggaggat gacggtcccc ccatgagaga gatcatactc 4500aaggtggtcc tgatgaccat ctgtggcatg aacccaatag ccataccctt tgcagctgga 4560gcgtggtacg tatacgtgaa gactggaaaa aggagtggtg ctctatggga tgtgcctgct 4620cccaaggaag taaaaaaggg ggagaccaca gatggagtgt acagagtaat gactcgtaga 4680ctgctaggtt caacacaagt tggagtggga gttatgcaag agggggtctt tcacactatg 4740tggcacgtca caaaaggatc cgcgctgaga agcggtgaag ggagacttga tccatactgg 4800ggagatgtca agcaggatct ggtgtcatac tgtggtccat ggaagctaga tgccgcctgg 4860gacgggcaca gcgaggtgca gctcttggcc gtgccccccg gagagagagc gaggaacatc 4920cagactctgc ccggaatatt taagacaaag gatggggaca ttggagcggt tgcgctggat 4980tacccagcag gaacttcagg atctccaatc ctagacaagt gtgggagagt gataggactt 5040tatggcaatg gggtcgtgat caaaaatggg agttatgtta gtgccatcac ccaagggagg 5100agggaggaag agactcctgt tgagtgcttc gagccttcga tgctgaagaa gaagcagcta 5160actgtcttag acttgcatcc tggagctggg aaaaccagga gagttcttcc tgaaatagtc 5220cgtgaagcca taaaaacaag actccgtact gtgatcttag ctccaaccag ggttgtcgct 5280gctgaaatgg aggaagccct tagagggctt ccagtgcgtt atatgacaac agcagtcaat 5340gtcacccact ctggaacaga aatcgtcgac ttaatgtgcc atgccacctt cacttcacgt 5400ctactacagc caatcagagt ccccaactat aatctgtata ttatggatga ggcccacttc 5460acagatccct caagtatagc agcaagagga tacatttcaa caagggttga gatgggcgag 5520gcggctgcca tcttcatgac cgccacgcca ccaggaaccc gtgacgcatt tccggactcc 5580aactcaccaa ttatggacac cgaagtggaa gtcccagaga gagcctggag ctcaggcttt 5640gattgggtga cggatcattc tggaaaaaca gtttggtttg ttccaagcgt gaggaacggc 5700aatgagatcg cagcttgtct gacaaaggct ggaaaacggg tcatacagct cagcagaaag 5760acttttgaga cagagttcca gaaaacaaaa catcaagagt gggactttgt cgtgacaact 5820gacatttcag agatgggcgc caactttaaa gctgaccgtg tcatagattc caggagatgc 5880ctaaagccgg tcatacttga tggcgagaga gtcattctgg ctggacccat gcctgtcaca 5940catgccagcg ctgcccagag gagggggcgc ataggcagga atcccaacaa acctggagat 6000gagtatctgt atggaggtgg gtgcgcagag actgacgaag accatgcaca ctggcttgaa 6060gcaagaatgc tccttgacaa tatttacctc caagatggcc tcatagcctc gctctatcga 6120cctgaggccg acaaagtagc agccattgag ggagagttca agcttaggac ggagcaaagg 6180aagacctttg tggaactcat gaaaagagga gatcttcctg tttggctggc ctatcaggtt 6240gcatctgccg gaataaccta cacagataga agatggtgct ttgatggcac gaccaacaac 6300accataatgg aagacagtgt gccggcagag gtgtggacca gacacggaga gaaaagagtg 6360ctcaaaccga ggtggatgga cgccagagtt tgttcagatc atgcggccct gaagtcattc 6420aaggagtttg ccgctgggaa aagaggagcg gcttttggag tgatggaagc cctgggaaca 6480ctgccaggac acatgacaga gagattccag gaagccattg acaacctcgc tgtgctcatg 6540cgggcagaga ctggaagcag gccttacaaa gccgcggcgg cccaattgcc ggagacccta 6600gagaccatta tgcttttggg gttgctggga acagtctcgc tgggaatctt tttcgtcttg 6660atgaggaaca agggcatagg gaagatgggc tttggaatgg tgactcttgg ggccagcgca 6720tggctcatgt

ggctctcgga aattgagcca gccagaattg catgtgtcct cattgttgtg 6780ttcctattgc tggtggtgct catacctgag ccagaaaagc aaagatctcc ccaggacaac 6840caaatggcaa tcatcatcat ggtagcagta ggtcttctgg gcttgattac cgccaatgaa 6900ctcggatggt tggagagaac aaagagtgac ctaagccatc taatgggaag gagagaggag 6960ggggcaacca taggattctc aatggacatt gacctgcggc cagcctcagc ttgggccatc 7020tatgctgcct tgacaacttt cattacccca gccgtccaac atgcagtgac cacttcatac 7080aacaactact ccttaatggc gatggccacg caagctggag tgttgtttgg tatgggcaaa 7140gggatgccat tctacgcatg ggactttgga gtcccgctgc taatgatagg ttgctactca 7200caattaacac ccctgaccct aatagtggcc atcattttgc tcgtggcgca ctacatgtac 7260ttgatcccag ggctgcaggc agcagctgcg cgtgctgccc agaagagaac ggcagctggc 7320atcatgaaga accctgttgt ggatggaata gtggtgactg acattgacac aatgacaatt 7380gacccccaag tggagaaaaa gatgggacag gtgctactca tagcagtagc cgtctccagc 7440gccatactgt cgcggaccgc ctgggggtgg ggggaggctg gggccctgat cacagcggca 7500acttccactt tgtgggaagg ctctccgaac aagtactgga actcctctac agccacttca 7560ctgtgtaaca tttttagggg aagttacttg gctggagctt ctctaatcta cacagtaaca 7620agaaacgctg gcttggtcaa gagacgtggg ggtggaacag gagagaccct gggagagaaa 7680tggaaggccc gcttgaacca gatgtcggcc ctggagttct actcctacaa aaagtcaggc 7740atcaccgagg tgtgcagaga agaggcccgc cgcgccctca aggacggtgt ggcaacggga 7800ggccatgctg tgtcccgagg aagtgcaaag ctgagatggt tggtggagcg gggatacctg 7860cagccctatg gaaaggtcat tgatcttgga tgtggcagag ggggctggag ttactacgcc 7920gccaccatcc gcaaagttca agaagtgaaa ggatacacaa aaggaggccc tggtcatgaa 7980gaacccatgt tggtgcaaag ctatgggtgg aacatagtcc gtcttaagag tggggtggac 8040gtctttcata tggcggctga gccgtgtgac acgttgctgt gtgacatagg tgagtcatca 8100tctagtcctg aagtggaaga agcacggacg ctcagagtcc tctccatggt gggggattgg 8160cttgaaaaaa gaccaggagc cttttgtata aaagtgttgt gcccatacac cagcactatg 8220atggaaaccc tggagcgact gcagcgtagg tatgggggag gactggtcag agtgccactc 8280tcccgcaact ctacacatga gatgtactgg gtctctggag cgaaaagcaa caccataaaa 8340agtgtgtcca ccacgagcca gctcctcttg gggcgcatgg acgggcccag gaggccagtg 8400aaatatgagg aggatgtgaa tctcggctct ggcacgcggg ctgtggtaag ctgcgctgaa 8460gctcccaaca tgaagatcat tggtaaccgc attgaaagga tccgcagtga gcacgcggaa 8520acgtggttct ttgacgagaa ccacccatat aggacatggg cttaccatgg aagctatgag 8580gcccccacac aagggtcagc gtcctctcta ataaacgggg ttgtcaggct cctgtcaaaa 8640ccctgggatg tggtgactgg agtcacagga atagccatga ccgacaccac accgtatggt 8700cagcaaagag ttttcaagga aaaagtggac actagggtgc cagaccccca agaaggcact 8760cgtcaggtta tgagcatggt ctcttcctgg ttgtggaaag agctaggcaa acacaaacgg 8820ccacgagtct gtaccaaaga agagttcatc aacaaggttc gtagcaatgc agcattaggg 8880gcaatatttg aagaggaaaa agagtggaag actgcagtgg aagctgtgaa cgatccaagg 8940ttctgggctc tagtggacaa ggaaagagag caccacctga gaggagagtg ccagagttgt 9000gtgtacaaca tgatgggaaa aagagaaaag aaacaagggg aatttggaaa ggccaagggc 9060agccgcgcca tctggtatat gtggctaggg gctagatttc tagagttcga agcccttgga 9120ttcttgaacg aggatcactg gatggggaga gagaactcag gaggtggtgt tgaagggctg 9180ggattacaaa gactcggata tgtcctagaa gagatgagtc gcataccagg aggaaggatg 9240tatgcagatg acactgctgg ctgggacacc cgcatcagca ggtttgatct ggagaatgaa 9300gctctaatca ccaaccaaat ggagaaaggg cacagggcct tggcattggc cataatcaag 9360tacacatacc aaaacaaagt ggtaaaggtc cttagaccag ctgaaaaagg gaagacagtt 9420atggacatta tttcgagaca agaccaaagg gggagcggac aagttgtcac ttacgctctt 9480aacacattta ccaacctagt ggtgcaactc attcggaata tggaggctga ggaagttcta 9540gagatgcaag acttgtggct gctgcggagg tcagagaaag tgaccaactg gttgcagagc 9600aacggatggg ataggctcaa acgaatggca gtcagtggag atgattgcgt tgtgaagcca 9660attgatgata ggtttgcaca tgccctcagg ttcttgaatg atatgggaaa agttaggaag 9720gacacacaag agtggaaacc ctcaactgga tgggacaact gggaagaagt tccgttttgc 9780tcccaccact tcaacaagct ccatctcaag gacgggaggt ccattgtggt tccctgccgc 9840caccaagatg aactgattgg ccgggcccgc gtctctccag gggcgggatg gagcatccgg 9900gagactgctt gcctagcaaa atcatatgcg caaatgtggc agctccttta tttccacaga 9960agggacctcc gactgatggc caatgccatt tgttcatctg tgccagttga ctgggttcca 10020actgggagaa ctacctggtc aatccatgga aagggagaat ggatgaccac tgaagacatg 10080cttgtggtgt ggaacagagt gtggattgag gagaacgacc acatggaaga caagacccca 10140gttacgaaat ggacagacat tccctatttg ggaaaaaggg aagacttgtg gtgtggatct 10200ctcatagggc acagaccgcg caccacctgg gctgagaaca ttaaaaacac agtcaacatg 10260gtgcgcagga tcataggtga tgaagaaaag tacatggact acctatccac ccaagttcgc 10320tacttgggtg aagaagggtc tacacctgga gtgctgtaag caccaatctt agtgttgtca 10380ggcctgctag tcagccacag cttggggaaa gctgtgcagc ctgtgacccc cccaggagaa 10440gctgggaaac caagcctata gtcaggccga gaacgccatg gcacggaaga agccatgctg 10500cctgtgagcc cctcagagga cactgagtca aaaaacccca cgcgcttgga ggcgcaggat 10560gggaaaagaa ggtggcgacc ttccccaccc ttcaatctgg ggcctgaact ggagatcagc 10620tgtggatctc cagaagaggg actagtggtt agaggagacc ccccggaaaa cgcaaaacag 10680catattgacg ctgggaaaga ccagagactc catgagtttc caccacgctg gccgccaggc 10740acagatcgcc gaatagcggc ggccggtgtg ggg 10773793423PRTZika virus 79Met Lys Asn Pro Lys Lys Lys Ser Gly Gly Phe Arg Ile Val Asn Met1 5 10 15Leu Lys Arg Gly Val Ala Arg Val Ser Pro Phe Gly Gly Leu Lys Arg 20 25 30Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 35 40 45Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 50 55 60Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile65 70 75 80Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 85 90 95Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile 100 105 110Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg 115 120 125Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala 130 135 140Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile145 150 155 160Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro 165 170 175Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn 180 185 190Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly 195 200 205Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr 210 215 220Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr225 230 235 240Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly 245 250 255Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr 260 265 270Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 275 280 285Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 290 295 300Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys305 310 315 320Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 325 330 335Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 340 345 350Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 355 360 365Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 370 375 380Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys385 390 395 400Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 405 410 415Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 420 425 430Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 435 440 445Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 450 455 460Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys465 470 475 480Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 485 490 495Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 500 505 510Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 515 520 525Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 530 535 540Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala545 550 555 560Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 565 570 575Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 580 585 590Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 595 600 605Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 610 615 620Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln625 630 635 640Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 645 650 655Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 660 665 670Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 675 680 685His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 690 695 700Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp705 710 715 720Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His 725 730 735Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 740 745 750Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn 755 760 765Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val 770 775 780Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val785 790 795 800Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly Thr Gly Val Phe Val Tyr 805 810 815Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr Lys Tyr His Pro Asp Ser 820 825 830Pro Arg Arg Leu Ala Ala Ala Val Lys Gln Ala Trp Glu Asp Gly Ile 835 840 845Cys Gly Ile Ser Ser Val Ser Arg Met Glu Asn Ile Met Trp Arg Ser 850 855 860Val Glu Gly Glu Leu Asn Ala Ile Leu Glu Glu Asn Gly Val Gln Leu865 870 875 880Thr Val Val Val Gly Ser Val Lys Asn Pro Met Trp Arg Gly Pro Gln 885 890 895Arg Leu Pro Val Pro Val Asn Glu Leu Pro His Gly Trp Lys Ala Trp 900 905 910Gly Lys Ser Tyr Phe Val Arg Ala Ala Lys Thr Asn Asn Ser Phe Val 915 920 925Val Asp Gly Asp Thr Leu Lys Glu Cys Pro Leu Lys His Arg Ala Trp 930 935 940Asn Ser Phe Leu Val Glu Asp His Gly Phe Gly Val Phe His Thr Ser945 950 955 960Val Trp Leu Lys Val Arg Glu Asp Tyr Ser Leu Glu Cys Asp Pro Ala 965 970 975Val Ile Gly Thr Ala Val Lys Gly Lys Glu Ala Val His Ser Asp Leu 980 985 990Gly Tyr Trp Ile Glu Ser Glu Lys Asn Asp Thr Trp Arg Leu Lys Arg 995 1000 1005Ala His Leu Ile Glu Met Lys Thr Cys Glu Trp Pro Lys Ser His 1010 1015 1020Thr Leu Trp Thr Asp Gly Ile Glu Glu Ser Asp Leu Ile Ile Pro 1025 1030 1035Lys Ser Leu Ala Gly Pro Leu Ser His His Asn Thr Arg Glu Gly 1040 1045 1050Tyr Arg Thr Gln Met Lys Gly Pro Trp His Ser Glu Glu Leu Glu 1055 1060 1065Ile Arg Phe Glu Glu Cys Pro Gly Thr Lys Val His Val Glu Glu 1070 1075 1080Thr Cys Gly Thr Arg Gly Pro Ser Leu Arg Ser Thr Thr Ala Ser 1085 1090 1095Gly Arg Val Ile Glu Glu Trp Cys Cys Arg Glu Cys Thr Met Pro 1100 1105 1110Pro Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp Tyr Gly Met Glu 1115 1120 1125Ile Arg Pro Arg Lys Glu Pro Glu Ser Asn Leu Val Arg Ser Met 1130 1135 1140Val Thr Ala Gly Ser Thr Asp His Met Asp His Phe Ser Leu Gly 1145 1150 1155Val Leu Val Ile Leu Leu Met Val Gln Glu Gly Leu Lys Lys Arg 1160 1165 1170Met Thr Thr Lys Ile Ile Ile Ser Thr Ser Met Ala Val Leu Val 1175 1180 1185Ala Met Ile Leu Gly Gly Phe Ser Met Ser Asp Leu Ala Lys Leu 1190 1195 1200Ala Ile Leu Met Gly Ala Thr Phe Ala Glu Met Asn Thr Gly Gly 1205 1210 1215Asp Val Ala His Leu Ala Leu Ile Ala Ala Phe Lys Val Arg Pro 1220 1225 1230Ala Leu Leu Val Ser Phe Ile Phe Arg Ala Asn Trp Thr Pro Arg 1235 1240 1245Glu Ser Met Leu Leu Ala Leu Ala Ser Cys Leu Leu Gln Thr Ala 1250 1255 1260Ile Ser Ala Leu Glu Gly Asp Leu Met Val Leu Ile Asn Gly Phe 1265 1270 1275Ala Leu Ala Trp Leu Ala Ile Arg Ala Met Val Val Pro Arg Thr 1280 1285 1290Asp Asn Ile Thr Leu Ala Ile Leu Ala Ala Leu Thr Pro Leu Ala 1295 1300 1305Arg Gly Thr Leu Leu Val Ala Trp Arg Ala Gly Leu Ala Thr Cys 1310 1315 1320Gly Gly Phe Met Leu Leu Ser Leu Lys Gly Lys Gly Ser Val Lys 1325 1330 1335Lys Asn Leu Pro Phe Val Met Ala Leu Gly Leu Thr Ala Val Arg 1340 1345 1350Leu Val Asp Pro Ile Asn Val Val Gly Leu Leu Leu Leu Thr Arg 1355 1360 1365Ser Gly Lys Arg Ser Trp Pro Pro Ser Glu Val Leu Thr Ala Val 1370 1375 1380Gly Leu Ile Cys Ala Leu Ala Gly Gly Phe Ala Lys Ala Asp Ile 1385 1390 1395Glu Met Ala Gly Pro Met Ala Ala Val Gly Leu Leu Ile Val Ser 1400 1405 1410Tyr Val Val Ser Gly Lys Ser Val Asp Met Tyr Ile Glu Arg Ala 1415 1420 1425Gly Asp Ile Thr Trp Glu Lys Asp Ala Glu Val Thr Gly Asn Ser 1430 1435 1440Pro Arg Leu Asp Val Ala Leu Asp Glu Ser Gly Asp Phe Ser Leu 1445 1450 1455Val Glu Asp Asp Gly Pro Pro Met Arg Glu Ile Ile Leu Lys Val 1460 1465 1470Val Leu Met Thr Ile Cys Gly Met Asn Pro Ile Ala Ile Pro Phe 1475 1480 1485Ala Ala Gly Ala Trp Tyr Val Tyr Val Lys Thr Gly Lys Arg Ser 1490 1495 1500Gly Ala Leu Trp Asp Val Pro Ala Pro Lys Glu Val Lys Lys Gly 1505 1510 1515Glu Thr Thr Asp Gly Val Tyr Arg Val Met Thr Arg Arg Leu Leu 1520 1525 1530Gly Ser Thr Gln Val Gly Val Gly Val Met Gln Glu Gly Val Phe 1535 1540 1545His Thr Met Trp His Val Thr Lys Gly Ser Ala Leu Arg Ser Gly 1550 1555 1560Glu Gly Arg Leu Asp Pro Tyr Trp Gly Asp Val Lys Gln Asp Leu 1565 1570 1575Val Ser Tyr Cys Gly Pro Trp Lys Leu Asp Ala Ala Trp Asp Gly 1580 1585 1590His Ser Glu Val Gln Leu Leu Ala Val Pro Pro Gly Glu Arg Ala 1595 1600 1605Arg Asn Ile Gln Thr Leu Pro Gly Ile Phe Lys Thr Lys Asp Gly 1610 1615 1620Asp Ile Gly Ala Val Ala Leu Asp Tyr Pro Ala Gly Thr Ser Gly 1625 1630 1635Ser Pro Ile Leu Asp Lys Cys Gly Arg Val Ile Gly Leu Tyr Gly 1640 1645 1650Asn Gly Val Val Ile Lys Asn Gly Ser Tyr Val Ser Ala Ile Thr 1655 1660 1665Gln Gly Arg Arg Glu Glu Glu Thr Pro Val Glu Cys Phe Glu Pro 1670 1675 1680Ser Met Leu Lys Lys Lys Gln Leu Thr Val Leu Asp Leu His Pro 1685 1690 1695Gly Ala Gly Lys Thr Arg Arg Val Leu Pro Glu Ile Val Arg Glu 1700 1705 1710Ala Ile Lys Thr Arg Leu Arg Thr Val Ile Leu Ala Pro Thr Arg 1715 1720 1725Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu Pro Val 1730 1735 1740Arg Tyr Met Thr Thr Ala Val Asn Val Thr His Ser Gly Thr Glu 1745 1750 1755Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Ser Arg Leu Leu 1760 1765 1770Gln Pro Ile Arg Val Pro Asn Tyr Asn Leu Tyr Ile Met Asp Glu 1775 1780 1785Ala

His Phe Thr Asp Pro Ser Ser Ile Ala Ala Arg Gly Tyr Ile 1790 1795 1800Ser Thr Arg Val Glu Met Gly Glu Ala Ala Ala Ile Phe Met Thr 1805 1810 1815Ala Thr Pro Pro Gly Thr Arg Asp Ala Phe Pro Asp Ser Asn Ser 1820 1825 1830Pro Ile Met Asp Thr Glu Val Glu Val Pro Glu Arg Ala Trp Ser 1835 1840 1845Ser Gly Phe Asp Trp Val Thr Asp His Ser Gly Lys Thr Val Trp 1850 1855 1860Phe Val Pro Ser Val Arg Asn Gly Asn Glu Ile Ala Ala Cys Leu 1865 1870 1875Thr Lys Ala Gly Lys Arg Val Ile Gln Leu Ser Arg Lys Thr Phe 1880 1885 1890Glu Thr Glu Phe Gln Lys Thr Lys His Gln Glu Trp Asp Phe Val 1895 1900 1905Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys Ala Asp 1910 1915 1920Arg Val Ile Asp Ser Arg Arg Cys Leu Lys Pro Val Ile Leu Asp 1925 1930 1935Gly Glu Arg Val Ile Leu Ala Gly Pro Met Pro Val Thr His Ala 1940 1945 1950Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg Asn Pro Asn Lys 1955 1960 1965Pro Gly Asp Glu Tyr Leu Tyr Gly Gly Gly Cys Ala Glu Thr Asp 1970 1975 1980Glu Asp His Ala His Trp Leu Glu Ala Arg Met Leu Leu Asp Asn 1985 1990 1995Ile Tyr Leu Gln Asp Gly Leu Ile Ala Ser Leu Tyr Arg Pro Glu 2000 2005 2010Ala Asp Lys Val Ala Ala Ile Glu Gly Glu Phe Lys Leu Arg Thr 2015 2020 2025Glu Gln Arg Lys Thr Phe Val Glu Leu Met Lys Arg Gly Asp Leu 2030 2035 2040Pro Val Trp Leu Ala Tyr Gln Val Ala Ser Ala Gly Ile Thr Tyr 2045 2050 2055Thr Asp Arg Arg Trp Cys Phe Asp Gly Thr Thr Asn Asn Thr Ile 2060 2065 2070Met Glu Asp Ser Val Pro Ala Glu Val Trp Thr Arg His Gly Glu 2075 2080 2085Lys Arg Val Leu Lys Pro Arg Trp Met Asp Ala Arg Val Cys Ser 2090 2095 2100Asp His Ala Ala Leu Lys Ser Phe Lys Glu Phe Ala Ala Gly Lys 2105 2110 2115Arg Gly Ala Ala Phe Gly Val Met Glu Ala Leu Gly Thr Leu Pro 2120 2125 2130Gly His Met Thr Glu Arg Phe Gln Glu Ala Ile Asp Asn Leu Ala 2135 2140 2145Val Leu Met Arg Ala Glu Thr Gly Ser Arg Pro Tyr Lys Ala Ala 2150 2155 2160Ala Ala Gln Leu Pro Glu Thr Leu Glu Thr Ile Met Leu Leu Gly 2165 2170 2175Leu Leu Gly Thr Val Ser Leu Gly Ile Phe Phe Val Leu Met Arg 2180 2185 2190Asn Lys Gly Ile Gly Lys Met Gly Phe Gly Met Val Thr Leu Gly 2195 2200 2205Ala Ser Ala Trp Leu Met Trp Leu Ser Glu Ile Glu Pro Ala Arg 2210 2215 2220Ile Ala Cys Val Leu Ile Val Val Phe Leu Leu Leu Val Val Leu 2225 2230 2235Ile Pro Glu Pro Glu Lys Gln Arg Ser Pro Gln Asp Asn Gln Met 2240 2245 2250Ala Ile Ile Ile Met Val Ala Val Gly Leu Leu Gly Leu Ile Thr 2255 2260 2265Ala Asn Glu Leu Gly Trp Leu Glu Arg Thr Lys Ser Asp Leu Ser 2270 2275 2280His Leu Met Gly Arg Arg Glu Glu Gly Ala Thr Ile Gly Phe Ser 2285 2290 2295Met Asp Ile Asp Leu Arg Pro Ala Ser Ala Trp Ala Ile Tyr Ala 2300 2305 2310Ala Leu Thr Thr Phe Ile Thr Pro Ala Val Gln His Ala Val Thr 2315 2320 2325Thr Ser Tyr Asn Asn Tyr Ser Leu Met Ala Met Ala Thr Gln Ala 2330 2335 2340Gly Val Leu Phe Gly Met Gly Lys Gly Met Pro Phe Tyr Ala Trp 2345 2350 2355Asp Phe Gly Val Pro Leu Leu Met Ile Gly Cys Tyr Ser Gln Leu 2360 2365 2370Thr Pro Leu Thr Leu Ile Val Ala Ile Ile Leu Leu Val Ala His 2375 2380 2385Tyr Met Tyr Leu Ile Pro Gly Leu Gln Ala Ala Ala Ala Arg Ala 2390 2395 2400Ala Gln Lys Arg Thr Ala Ala Gly Ile Met Lys Asn Pro Val Val 2405 2410 2415Asp Gly Ile Val Val Thr Asp Ile Asp Thr Met Thr Ile Asp Pro 2420 2425 2430Gln Val Glu Lys Lys Met Gly Gln Val Leu Leu Ile Ala Val Ala 2435 2440 2445Val Ser Ser Ala Ile Leu Ser Arg Thr Ala Trp Gly Trp Gly Glu 2450 2455 2460Ala Gly Ala Leu Ile Thr Ala Ala Thr Ser Thr Leu Trp Glu Gly 2465 2470 2475Ser Pro Asn Lys Tyr Trp Asn Ser Ser Thr Ala Thr Ser Leu Cys 2480 2485 2490Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Ser Leu Ile Tyr 2495 2500 2505Thr Val Thr Arg Asn Ala Gly Leu Val Lys Arg Arg Gly Gly Gly 2510 2515 2520Thr Gly Glu Thr Leu Gly Glu Lys Trp Lys Ala Arg Leu Asn Gln 2525 2530 2535Met Ser Ala Leu Glu Phe Tyr Ser Tyr Lys Lys Ser Gly Ile Thr 2540 2545 2550Glu Val Cys Arg Glu Glu Ala Arg Arg Ala Leu Lys Asp Gly Val 2555 2560 2565Ala Thr Gly Gly His Ala Val Ser Arg Gly Ser Ala Lys Leu Arg 2570 2575 2580Trp Leu Val Glu Arg Gly Tyr Leu Gln Pro Tyr Gly Lys Val Ile 2585 2590 2595Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Ala Ala Thr 2600 2605 2610Ile Arg Lys Val Gln Glu Val Lys Gly Tyr Thr Lys Gly Gly Pro 2615 2620 2625Gly His Glu Glu Pro Met Leu Val Gln Ser Tyr Gly Trp Asn Ile 2630 2635 2640Val Arg Leu Lys Ser Gly Val Asp Val Phe His Met Ala Ala Glu 2645 2650 2655Pro Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Ser Ser 2660 2665 2670Pro Glu Val Glu Glu Ala Arg Thr Leu Arg Val Leu Ser Met Val 2675 2680 2685Gly Asp Trp Leu Glu Lys Arg Pro Gly Ala Phe Cys Ile Lys Val 2690 2695 2700Leu Cys Pro Tyr Thr Ser Thr Met Met Glu Thr Leu Glu Arg Leu 2705 2710 2715Gln Arg Arg Tyr Gly Gly Gly Leu Val Arg Val Pro Leu Ser Arg 2720 2725 2730Asn Ser Thr His Glu Met Tyr Trp Val Ser Gly Ala Lys Ser Asn 2735 2740 2745Thr Ile Lys Ser Val Ser Thr Thr Ser Gln Leu Leu Leu Gly Arg 2750 2755 2760Met Asp Gly Pro Arg Arg Pro Val Lys Tyr Glu Glu Asp Val Asn 2765 2770 2775Leu Gly Ser Gly Thr Arg Ala Val Val Ser Cys Ala Glu Ala Pro 2780 2785 2790Asn Met Lys Ile Ile Gly Asn Arg Ile Glu Arg Ile Arg Ser Glu 2795 2800 2805His Ala Glu Thr Trp Phe Phe Asp Glu Asn His Pro Tyr Arg Thr 2810 2815 2820Trp Ala Tyr His Gly Ser Tyr Glu Ala Pro Thr Gln Gly Ser Ala 2825 2830 2835Ser Ser Leu Ile Asn Gly Val Val Arg Leu Leu Ser Lys Pro Trp 2840 2845 2850Asp Val Val Thr Gly Val Thr Gly Ile Ala Met Thr Asp Thr Thr 2855 2860 2865Pro Tyr Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg 2870 2875 2880Val Pro Asp Pro Gln Glu Gly Thr Arg Gln Val Met Ser Met Val 2885 2890 2895Ser Ser Trp Leu Trp Lys Glu Leu Gly Lys His Lys Arg Pro Arg 2900 2905 2910Val Cys Thr Lys Glu Glu Phe Ile Asn Lys Val Arg Ser Asn Ala 2915 2920 2925Ala Leu Gly Ala Ile Phe Glu Glu Glu Lys Glu Trp Lys Thr Ala 2930 2935 2940Val Glu Ala Val Asn Asp Pro Arg Phe Trp Ala Leu Val Asp Lys 2945 2950 2955Glu Arg Glu His His Leu Arg Gly Glu Cys Gln Ser Cys Val Tyr 2960 2965 2970Asn Met Met Gly Lys Arg Glu Lys Lys Gln Gly Glu Phe Gly Lys 2975 2980 2985Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg 2990 2995 3000Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp 3005 3010 3015Met Gly Arg Glu Asn Ser Gly Gly Gly Val Glu Gly Leu Gly Leu 3020 3025 3030Gln Arg Leu Gly Tyr Val Leu Glu Glu Met Ser Arg Ile Pro Gly 3035 3040 3045Gly Arg Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile 3050 3055 3060Ser Arg Phe Asp Leu Glu Asn Glu Ala Leu Ile Thr Asn Gln Met 3065 3070 3075Glu Lys Gly His Arg Ala Leu Ala Leu Ala Ile Ile Lys Tyr Thr 3080 3085 3090Tyr Gln Asn Lys Val Val Lys Val Leu Arg Pro Ala Glu Lys Gly 3095 3100 3105Lys Thr Val Met Asp Ile Ile Ser Arg Gln Asp Gln Arg Gly Ser 3110 3115 3120Gly Gln Val Val Thr Tyr Ala Leu Asn Thr Phe Thr Asn Leu Val 3125 3130 3135Val Gln Leu Ile Arg Asn Met Glu Ala Glu Glu Val Leu Glu Met 3140 3145 3150Gln Asp Leu Trp Leu Leu Arg Arg Ser Glu Lys Val Thr Asn Trp 3155 3160 3165Leu Gln Ser Asn Gly Trp Asp Arg Leu Lys Arg Met Ala Val Ser 3170 3175 3180Gly Asp Asp Cys Val Val Lys Pro Ile Asp Asp Arg Phe Ala His 3185 3190 3195Ala Leu Arg Phe Leu Asn Asp Met Gly Lys Val Arg Lys Asp Thr 3200 3205 3210Gln Glu Trp Lys Pro Ser Thr Gly Trp Asp Asn Trp Glu Glu Val 3215 3220 3225Pro Phe Cys Ser His His Phe Asn Lys Leu His Leu Lys Asp Gly 3230 3235 3240Arg Ser Ile Val Val Pro Cys Arg His Gln Asp Glu Leu Ile Gly 3245 3250 3255Arg Ala Arg Val Ser Pro Gly Ala Gly Trp Ser Ile Arg Glu Thr 3260 3265 3270Ala Cys Leu Ala Lys Ser Tyr Ala Gln Met Trp Gln Leu Leu Tyr 3275 3280 3285Phe His Arg Arg Asp Leu Arg Leu Met Ala Asn Ala Ile Cys Ser 3290 3295 3300Ser Val Pro Val Asp Trp Val Pro Thr Gly Arg Thr Thr Trp Ser 3305 3310 3315Ile His Gly Lys Gly Glu Trp Met Thr Thr Glu Asp Met Leu Val 3320 3325 3330Val Trp Asn Arg Val Trp Ile Glu Glu Asn Asp His Met Glu Asp 3335 3340 3345Lys Thr Pro Val Thr Lys Trp Thr Asp Ile Pro Tyr Leu Gly Lys 3350 3355 3360Arg Glu Asp Leu Trp Cys Gly Ser Leu Ile Gly His Arg Pro Arg 3365 3370 3375Thr Thr Trp Ala Glu Asn Ile Lys Asn Thr Val Asn Met Val Arg 3380 3385 3390Arg Ile Ile Gly Asp Glu Glu Lys Tyr Met Asp Tyr Leu Ser Thr 3395 3400 3405Gln Val Arg Tyr Leu Gly Glu Glu Gly Ser Thr Pro Gly Val Leu 3410 3415 34208028DNAArtificial Sequenceprimer 80ttaggatccg ttgttgatct gtgtgaat 288126DNAArtificial Sequenceprimer 81taactcgagc gtacacaacc caagtt 268227DNAArtificial Sequenceprimer 82ttaggatcct cactagacgt gggagtg 278329DNAArtificial Sequenceprimer 83taactcgaga agccatgtcy gatattgat 298426DNAArtificial Sequenceprimer 84ttaggatccg catacagcat caggtg 268527DNAArtificial Sequenceprimer 85taactcgagt gtggagttcc ggtgtct 278631DNAArtificial Sequenceprimer 86ttaggatccg aatagagcga argttgagat a 318728DNAArtificial Sequenceprimer 87taactcgagt ggtgggtgat cttcttct 288831DNAArtificial Sequenceprimer 88ttaggatcca gtcacagtgg aggtacagta c 318927DNAArtificial Sequenceprimer 89taactcgagc rcagatacca tcttccc 279028DNAArtificial Sequenceprimer 90ttaggatccc ttatgtgctt ggccttag 289126DNAArtificial Sequenceprimer 91taactcgagt cttcagcctc catgtg 269229DNAArtificial Sequenceprimer 92ttaggatcca atgcccactc aaacataga 299330DNAArtificial Sequenceprimer 93taactcgagt cattctcttc ttcagccctt 309426DNAArtificial Sequenceprimer 94ttaggatcca agggtgatcg aggaat 269529DNAArtificial Sequenceprimer 95taactcgagt tcccttcaga gagaggagc 299628DNAArtificial Sequenceprimer 96ttaggatcct cttttgcaaa ctgcgatc 289727DNAArtificial Sequenceprimer 97taactcgagt ccagctgcaa agggtat 279828DNAArtificial Sequenceprimer 98ttaggatccg tgtggacatg tacattga 289926DNAArtificial Sequenceprimer 99taactcgagc ccattgccat aaagtc 2610028DNAArtificial Sequenceprimer 100ttaggatcct catactgtgg tccatgga 2810126DNAArtificial Sequenceprimer 101taactcgagg cccatctcaa cccttg 2610226DNAArtificial Sequenceprimer 102ttaggatcct agagggcttc cagtgc 2610331DNAArtificial Sequenceprimer 103taactcgaga tactcatctc caggtttgtt g 3110430DNAArtificial Sequenceprimer 104ttaggatccg aaaacaaaac atcaagagtg 3010531DNAArtificial Sequenceprimer 105taactcgagg aatctctctg tcatgtgtcc t 3110626DNAArtificial Sequenceprimer 106ttaggatcct tgatggcacg accaac 2610728DNAArtificial Sequenceprimer 107ttaggatccg ttgttgatct gtgtgaat 2810826DNAArtificial Sequenceprimer 108taactcgagc aggtcaatgt ccattg 2610930DNAArtificial Sequenceprimer 109ttaggatcct gttgtgttcc tattgctggt 3011025DNAArtificial Sequenceprimer 110taactcgagt gatcagrgcc ccagc 2511126DNAArtificial Sequenceprimer 111ttaggatcct gctgcccaga agagaa 2611226DNAArtificial Sequenceprimer 112taactcgagc accaacaygg gttctt 2611325DNAArtificial Sequenceprimer 113ttaggatcct caaggacggt gtggc 2511428DNAArtificial Sequenceprimer 114taactcgagc aatgatcttc atgttggg 2811526DNAArtificial Sequenceprimer 115ttaggatcct atgggggagg actggt 2611625DNAArtificial Sequenceprimer 116taactcgagc ccagaacctt ggatc 2511725DNAArtificial Sequenceprimer 117ttaggatcca gacccccaag aaggc 2511826DNAArtificial Sequenceprimer 118taactcgagc ccctttggtc ttgtct 2611929DNAArtificial Sequenceprimer 119ttaggatcca ggaaggatgt atgcagatg 2912030DNAArtificial Sequenceprimer 120taactcgaga catttgcgca tatgattttg 3012128DNAArtificial Sequenceprimer 121ttaggatcca ggaaggacac acaagagt 2812226DNAArtificial Sequenceprimer 122taactcgaga caggctgcac agcttt 2612328DNAArtificial Sequenceprimer 123ttaggatcct ctctcatagg gcacagac 28

Claims

What is claimed is:

1. A method for purifying infectious virus particles, comprising the steps of (A) providing a crude harvest (a) comprising infectious virus particles, non-infectious virus particles, and impurities, wherein the impurities are generated from growing said virus particles on a cell substrate; (B) contacting said crude harvest (a) with an agent comprising protamine to obtain a virus preparation (b) comprising infectious virus particles; and (C) further purifying said virus preparation (b) by one or more size exclusion methods wherein said one or more size exclusion methods comprise (i) sucrose density gradient centrifugation, and/or (ii) a solid-phase matrix packed in a column comprising a ligand-activated core and an inactive shell comprising pores, wherein the pores comprise a molecular weight cut-off that excludes the virus particles from entering the ligand-activated core, and wherein a molecule smaller than the molecular weight cut-off of the pores can enter the ligand-activated core, and/or (iii) size exclusion chromatography; to obtain a final virus preparation (c) comprising the infectious virus particles, less than 100 ng/mL residual host cell DNA, less than 1 .mu.g/mL residual host cell protein, and less than 1 .mu.g/mL residual aggregates of virus particles are selected from the group consisting of filovirus particles of a flavivirus or alphavirus particles of a alphavirus but; wherein the virus particles are not of a Japanese encephalitis virus.

2. The method according to claim 1, wherein the final virus preparation (c) comprises less than 10 ng/mL residual host cell DNA and less than 100 ng/mL residual host cell protein.

3. The method according to claim 1, wherein said crude harvest (a) is subjected to one or more pre-purification step(s) prior to step (B), wherein the one or more pre-purification step(s) comprise a) filtration using a filter having a pore size equal to or less than 0.2 .mu.m; and/or b) digestion of host cell genomic DNA by enzymatic treatment; and/or c) ultra/diafiltration using a hollow fiber membrane having a pore size equal to or greater than 100 kDa.

4. The method according to claim 1, wherein the concentration of protamine is between 0.5 mg/ml and 3 mg/ml.

5. The method according to claim 1, wherein the infectious virus particles in said final virus preparation (c) are enriched by at least 50% to 95% relative to total virus particles in said crude harvest (a).

6. The method according to claim 1, wherein said virus preparation (c) comprises less than 10% impurities.

7. The method according to claim 1, wherein said infectious virus particles are propagated in a cell line selected from the group consisting of an EB66 cell line, a Vero cell line, a Vero-.alpha.His cell line, a HeLa cell line, a HeLa-S3 cell line, a 293 cell line, a PC12 cell line, a CHO cell line, a 3T3 cell line, a PerC6 cell line, an MDSK cell line, a chicken embryonic fibroblast cell line, a duck cell line, and a diploid avian cell line.

8. The method according to claim 1, wherein said infectious virus particles are selected from the group consisting of a live virus, an attenuated live virus, a chimeric virus, a modified live virus and a recombinant live virus.

9. The method according to claim 8, wherein said infectious virus particles are selected from the group of viruses consisting of Zika virus, Chikungunya virus, yellow fever virus, and Dengue virus.

10. The method according to claim 1, further comprising a step (D) comprising inactivating the final virus preparation (c).

11. The method according to claim 9, wherein said Chikungunya virus is an attenuated Chikungunya virus comprising a deletion mutation in the non-structural protein 3 provided by SEQ ID NO: 77 or an immunogenic variant thereof, wherein said immunogenic variant is defined as having at least 80% sequence identity to SEQ ID NO: 77.

12. The method according to claim 1, wherein said protamine is selected from the group comprising a protamine salt, a protamine sulphate and a recombinant protamine sulphate.

13. The method according to claim 10, wherein said inactivating step (D) is performed using formaldehyde.

14. The method according to claim 1, wherein said infectious virus particles are selected from the group consisting of flaviviruses and flaviviruses but not Japanese encephalitis virus.

Images