U.S. patent application number 14/275562 was filed with the patent office on 2014-09-04 for use of pyridone derivatives in the prevention or treatment of tissue or organ toxicity induced by cytotoxic agents and radiation.
This patent application is currently assigned to Shanghai Genomics, Inc.. The applicant listed for this patent is Shanghai Genomics, Inc.. Invention is credited to Jun Wu.
Application Number | 20140249347 14/275562 |
Document ID | / |
Family ID | 38833053 |
Filed Date | 2014-09-04 |
United States Patent
Application |
20140249347 |
Kind Code |
A1 |
Wu; Jun |
September 4, 2014 |
USE OF PYRIDONE DERIVATIVES IN THE PREVENTION OR TREATMENT OF
TISSUE OR ORGAN TOXICITY INDUCED BY CYTOTOXIC AGENTS AND
RADIATION
Abstract
The present invention is directed to a novel use of pyridone
derivatives such as pirfenidone for the prevention and treatment of
damages to tissues or organs induced by various cytotoxic agents,
such as chemotherapeutic agents, biologics, immunosuppressants and
radiation. Such prophylactic and/or therapeutic effects of the
pyridone derivatives make it possible to increase therapeutic
dosages of the cytotoxic agent, thereby enhancing the therapeutic
efficacy of the cytotoxic agent and radiation therapy.
Inventors: |
Wu; Jun; (Shanghai,
CN) |
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Applicant: |
Name |
City |
State |
Country |
Type |
Shanghai Genomics, Inc. |
Shanghai |
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CN |
|
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Assignee: |
Shanghai Genomics, Inc.
Shanghai
CN
|
Family ID: |
38833053 |
Appl. No.: |
14/275562 |
Filed: |
May 12, 2014 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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11958353 |
Dec 17, 2007 |
8765726 |
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14275562 |
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PCT/CN2006/002504 |
Sep 25, 2006 |
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11958353 |
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60804914 |
Jun 15, 2006 |
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Current U.S.
Class: |
600/1 ; 514/32;
514/34; 514/345 |
Current CPC
Class: |
A61K 31/7064 20130101;
A61K 31/704 20130101; A61K 31/4418 20130101; A61P 43/00 20180101;
A61P 39/00 20180101; A61N 5/10 20130101; A61P 11/00 20180101; A61P
31/00 20180101 |
Class at
Publication: |
600/1 ; 514/345;
514/32; 514/34 |
International
Class: |
A61K 31/4418 20060101
A61K031/4418; A61K 31/704 20060101 A61K031/704; A61N 5/10 20060101
A61N005/10; A61K 31/7064 20060101 A61K031/7064 |
Claims
1. A method for treating tissue damage associated with a cytotoxic
agent, an immunosuppressant, and/or radiation in a mammal in need
thereof, comprising: administering to the mammal a therapeutically
effective amount of a compound of formula I or a pharmaceutically
acceptable salt thereof, ##STR00009## wherein R.sub.1 is methyl,
ethyl or trifluoromethyl at position 3, 4, 5 or 6; and R.sub.2 is
hydroxyl, sulfydryl, C.sub.1-6 alkoxyl such as methoxyl and
ethoxyl, or a C.sub.1-6 alkylthio group such as a methylthio group
or an ethylthio group at position 2, 3 or 4; or, R.sub.2 is
absent.
2. The method of claim 1, wherein R.sub.1 is methyl, and R.sub.2 is
absent.
3. The method of claim 1, wherein R.sub.1 is methyl, and R.sub.2 is
hydroxyl.
4. The method of claim 1, wherein the compound is pirfenidone.
5. The method of claim 1, wherein the compound is
5-methyl-1-(4-hydroxylphenyl)-2-(1H)-pyridone (F351).
6. The method of claim 1, wherein the mammal is a human.
7. The method of claim 1, wherein the compound or its
pharmaceutically acceptable salt is administered simultaneously
with or after the mammal is administered the cytotoxic agent,
immunosuppressant, or radiation.
8. The method of claim 1, wherein the tissue damage is fibrosis or
inflammation of the kidney or lung.
9. The method of claim 1, wherein the tissue damage is radiation
pneumonitis, interstitial pneumonia, or pulmonary fibrosis.
10. A method of administering an increased dosage (D.sub.1) of
radiotherapy or a chemotherapeutic agent to a mammal in need
thereof, comprising: (a) administering to the mammal a
therapeutically-effective amount of a compound of formula I or a
pharmaceutically acceptable salt thereof, ##STR00010## wherein
R.sub.1 is methyl, ethyl or trifluoromethyl at position 3, 4, 5 or
6; and R.sub.2 is hydroxyl, sulfydryl, C.sub.1-6 alkoxyl such as
methoxyl and ethoxyl, or a C.sub.1-6 alkylthio group such as a
methylthio group or an ethylthio group at position 2, 3 or 4; or,
R.sub.2 is absent, and (b) administering to the mammal the
increased dosage (D.sub.1) of radiotherapy or the chemotherapeutic
agent, wherein the increased dosage (D.sub.1) is greater than a
dosage administered in the absence of a compound of Formula I
(D.sub.0).
11. The method of claim 10, wherein the irradiation dose D.sub.1
and D.sub.0 fit the following equation:
(D.sub.1-D.sub.0)/D.sub.0.gtoreq.20%.
12. The method of claim 10, wherein the chemotherapeutic dose
D.sub.1 and D.sub.0 fit the following equation:
(D.sub.1-D.sub.0)/D.sub.0.gtoreq.20%.
13. The method of claim 10, wherein R.sub.1 is methyl, and R.sub.2
is absent.
14. The method of claim 10, wherein R.sub.1 is methyl, and R.sub.2
is hydroxyl.
15. The method of claim 10, wherein the compound of Formula I is
pirfenidone or 5-methyl-1-(4-hydroxylphenyl)-2-(1H)-pyridone
(F351).
16. A method for treating pulmonary fibrosis or kidney fibrosis
associated with a cytotoxic agent, an immunosuppressant, and/or
radiation in a mammal in need thereof, comprising: administering to
the mammal a therapeutically-effective amount of a compound of
formula I or a pharmaceutically acceptable salt thereof,
##STR00011## wherein R.sub.1 is methyl, ethyl or trifluoromethyl at
position 3, 4, 5 or 6; and R.sub.2 is hydroxyl, sulfydryl,
C.sub.1-6 alkoxyl such as methoxyl and ethoxyl, or a C.sub.1-6
alkylthio group such as a methylthio group or an ethylthio group at
position 2, 3 or 4; or, R.sub.2 is absent.
17. The method of claim 16, wherein the compound of Formula I is
pirfenidone or 5-methyl-1-(4-hydroxylphenyl)-2-(1H)-pyridone
(F351).
18. A method for treating pneumonitis associated with a cytotoxic
agent, an immunosuppressant, and/or radiation in a mammal in need
thereof, comprising: administering to the mammal a
therapeutically-effective amount of a compound of Formula I or a
pharmaceutically acceptable salt thereof, ##STR00012## wherein
R.sub.1 is methyl, ethyl or trifluoromethyl at position 3, 4, 5 or
6; and R.sub.2 is hydroxyl, sulfydryl, C.sub.1-6 alkoxyl such as
methoxyl and ethoxyl, or a C.sub.1-6 alkylthio group such as a
methylthio group or an ethylthio group at position 2, 3 or 4; or,
R.sub.2 is absent.
19. The method of claim 18, wherein the compound of Formula I is
5-methyl-1-(4-hydroxylphenyl)-2-(1H)-pyridone (F351).
Description
CROSS REFERENCE TO RELATED APPLICATION
[0001] This application is a continuation of U.S. patent
application Ser. No. 11/958,353, filed on Dec. 17, 2007, which is a
continuation-in-part of PCT Application PCT/CN2006/002504 filed on
Sep. 25, 2006, which claims priority to U.S. Provisional
Application No. 60/804,914, filed on Jun. 15, 2006, all of which
applications are incorporated herein by reference in their
entirety.
FIELD OF THE INVENTION
[0002] The present invention relates to the pharmaceutical field,
and in particular, to a new use of pyridone derivatives in the
prevention and treatment of tissue or organ toxicity induced by
various cytotoxic agents, immunosuppressants, such as
chemotherapeutic agents, biologics and radiation.
BACKGROUND OF THE INVENTION
General Background
[0003] A wide variety of cytotoxic agents or therapy, such as
chemotherapy, immunosuppressive agents, biologics, radiotherapy and
combined therapy thereof, are used in the treatment of cancer and
many other diseases. However, when cancer cells are killed, normal
cells are also damaged simultaneously when the treatment is used on
human body. Typically, a series of toxic side effects occur, for
example, the damage on important tissues and/or organs, such as
heart, liver, kidney, lung, bone marrow, etc.
[0004] Radiation pneumonitis frequently appears after irradiation
of the lung area during the treatment of lung cancer, breast
cancer, and esophageal cancer. Radiation pneumonitis can lead to
life threatening fibrotic lesion in the lung. Usually, the severity
of pulmonary fibrosis is correlated with irradiation dosage. In
addition, abdominal irradiation may induce hepatitis, enteritis,
necrosis and fibrosis; whereas cavitas pelvis irradiation may
induce rectitis and cystitis, of which the resulted mild symptom is
mucosal dropsy and in the severe case is fibrosis.
[0005] The manifestations of pulmonary toxicity caused by drugs
used in chemotherapy are interstitial pneumonia and pulmonary
fibrosis. Drugs like Bleomycin, BCNU, MTX, Mitomycin, should be
withdrawn once pulmonary toxicity appears, followed by further
treatment with hormone, antibiotic, vitamin, etc. Drugs inducing
liver toxicity include BCNU, CCNU, Ara-C, L-ASP, VP-16, 6-MP, high
dose of MTX, CTX, DDP, DNR, Act-D, STZ, VCR, etc. Liver toxicity is
divided into three types: (1) hepatocyte function defect and
chemical hepatitis; (2) venous occlusion diseases; and (3) chronic
liver fibrosis.
[0006] Drugs used in chemotherapy also cause renal toxicity. Most
cytotoxic drugs, such as DDP, MTH, STZ, IFO, high dose of MTX,
cause renal functional defect by damaging renal glomerulus, rather
than renal tubule. Renal toxicity may occur immediately after drug
administration or develops subsequently, after long-term medicine
treatment or after withdrawal of the drugs.
[0007] Toxicity induced by combined chemotherapy and radiotherapy
is divided into early stage and late stage, of which the late stage
mainly involves damages of connective tissue within heart, kidney,
lung, etc. Interstitial cardiac fibrosis may occur after three
months following radiotherapy. For example, combined therapy of
adriamycin and radiotherapy may increase associated cardiac
toxicity. Drugs used in chemotherapy, such as bleomycin, adriamycin
and mitomycin, may increase the incidence rate and severity of
radiation pneumonitis and pulmonary fibrosis.
Injury of Organs Induced by Radiation
[0008] Radiotherapy is a common means for the treatment of
malignant tumors. Radiation on pulmonary carcinoma, mammary
adenocarcinoma, esophageal carcinoma, malignant lymphoma, or other
thoracic malignant tumors will also damage normal pulmonary tissue
in the irradiation filed, resulting in inflammation, which is
termed as radiation-induced lung injuries, i.e. acute radiation
pneumonitis.
[0009] Radiation pneumonitis is a major detrimental side effect
during radiotherapy, including early change (radiation pneumonitis)
and advanced change (radiation fibrosis). Everyone who has
undergone a pulmonary irradiation will have such change. In most
cases, such change is asymptomatic, but a change accompanied by
infection will produce symptoms, which is called acute radiation
pneumonitis. Asymptomatic inflammation will be gradually absorbed
and disappear to form various degrees of pulmonary parenchyma
fibrosis after completion of irradiation. Pulmonary fibrosis occurs
within about 6 months after irradiation, and then gradually
deteriorates to be worst within 1 year. Pathological changes of the
acute phase of radiation pneumonitis occur mostly within 1 to 2
months after irradiation, but in some cases they are observed after
a half year post radiotherapy. They manifest themselves as
congestion, edema and cellular infiltration due to damage of
pulmonary vessels (especially capillary vessel), decreased
reproduction of type II alveolar cells, lymphangiectasis, and
formation of hyaline membrane. The acute changes may spontaneously
disappear, but they generally cause desmoplasia, fibrosis, and
hyalinization of the lung. The chronic phase generally occurs after
9 months of irradiation, the pathologic of which is extensive
fibrosis of pulmonary alveolus, contraction of lung, inner membrane
thickening, stiffing, narrowing or obstruction of the lumen of
blood capillary resulting in increased resistance force of
pulmonary circulation and pulmonary artery hypertension. Pleura can
also thicken as the result of inflammation and fibrosis. Epithelial
anaplasia of the bronchiolar mucous lining and secondary pulmonary
infection can promote radiation fibrosis.
[0010] Development of radiation pneumonitis is mostly correlate
with the irradiation volume, while the other factors, such as
dosage and separation, conditions of the individual, individual
differences, and the presence of chronic lung diseases can also
contribute. Use of anti-cancer agents, such as ADM, PYM, and VCR
concurrently with radiation, as well as smoking, will also promote
development of radiation pneumonitis.
[0011] The severity of lung injuries is closely correlated with the
area of the lung irradiated, and the dose and rate of exposure. The
pathological changes are exudative inflammation of the acute phase
and extensive pulmonary fibrosis of the chronic phase. The larger
the dose received (more than 20Gy), the more severe the radiation
pneumonitis, eventually leading to the extensive pulmonary
fibrosis. The occurrence of radiation pneumonitis greatly hampers
the treatment of thoracic malignancies which are clinically common
and progress rapidly, and thus negatively impacts patients' quality
of life, or even threatens their lives. The incidence of radiation
pneumonitis reportedly varies, generally in the range of about
8.25% to 58%.
[0012] Currently, there are not clinically available medicaments
specifically for radiation-induced lung injuries. The common
treatments include: i) corticoid therapy, to control inflammation;
ii) anticoagulant therapy, to prevent small vascular embolism; iii)
high concentration oxygen therapy, to alleviate hyoxemia; and iv)
antibiotic therapy, to prevent secondary infection. The principle
of administration is: i) prednisone or dexamethasone orally
administered for general patients of radiation pneumonitis; ii)
dexamethasone administered by intravenous infusion for severe
patients; iii) antibiotics concurrently administered for patients
who have concomitant pulmonary infection. However, the effect of
hormone treatment is still in question, because some investigations
have showed that hormone treatment has no effect on
radiation-induced lung injuries.
[0013] It is undoubted that radiotherapy plays an important role in
the treatment of malignancies, however, its side effects of
irradiative responses and injuries are also noteworthy. High dose
of irradiation damages tumor cells as well as normal cells, causing
detrimental responses in the patients. If the irradiation dose is
not limited, it will kill normal cells when it kills the tumor.
Therefore, radiation therapy can only kill tumor cells to the most
extent where it is tolerated by normal tissues. In the situation of
a radio-sensitive tumor, a tumor-killing dose will not damage
normal tissues around the tumor, and hence the radiation therapy
can cure the tumor. While for a tumor whose fatal irradiation dose
can also kill or damage the surrounding normal tissue, normal
tissue will be greatly damaged at the same time with tumor cells.
Therefore, it is necessary to utilize suitable treatment modalities
to increase the ratio of the doses received by tumor versus normal
tissue so as to cure the tumor but not overly influence the normal
tissue. In addition, there is an earnest need in the art to develop
a method with increased irradiation dose and decreased
radiation-induced injuries.
[0014] In summary, cytotoxic therapy such as chemotherapy and
radiotherapy often causes acute tissue injury and fibrosis, thus
limiting the efficacy and safety of the treatment. Currently there
is no effective prophylactic or treatment regimen available for
such side effects during the treatment using a cytotoxic
therapy.
SUMMARY OF THE INVENTION
[0015] The present invention provides innovative compositions, kits
and methods for preventing, alleviating or treating of damages to
tissues or organs induced by various cytotoxic agents,
immunosuppressants or therapy, such as chemotherapeutic agents,
biologics and radiation.
[0016] In one aspect of the invention, the present invention
provides a method for preventing, treating or reducing damage to a
tissue or organ of a mammal who has been, is being, or will be
treated with a cytotoxic agent, an immunosuppressant, and/or
radiation, comprising: administering to the mammal a
therapeutically effective amount of a compound of Formula I or a
pharmaceutically acceptable salt thereof,
##STR00001##
wherein R.sub.1 is methyl, ethyl or trifluoromethyl at position 3,
4, 5 or 6; and R.sub.2 is hydroxyl, sulfydryl, C.sub.1-6 alkoxyl
such as methoxyl and ethoxyl, or a C.sub.1-6 alkylthio group such
as a methylthio group or an ethylthio group at position 2, 3 or 4;
or, R.sub.2 is absent.
[0017] In a preferred embodiment, the compound is pirfenidone.
[0018] In another preferred embodiment, the compound is
5-methyl-1-(4-hydroxylphenyl)-2-(1H)-pyridone, which is called
F351.
[0019] According to the method, the cytotoxic agent may be a
chemotherapeutic agent such as alkylating agents, antimetabolites,
and hormonal agents. The biologic may be a therapeutic antibody or
cytokine The radiation may be ionizing radiation.
[0020] The mammal may be a rodent such as a mouse or rat, but
preferably a human, more preferably a human patient having cancer,
tumor or in need of a tissue or organ transplant.
[0021] In one embodiment, the compound of Formula I is administered
prior to the treatment of the cytotoxic agent, immunosuppressant,
or radiation.
[0022] In another embodiment, the compound of Formula I is
administered on the same day when the mammal is treated with the
cytotoxic agent, immunosuppresant, or radiation.
[0023] In yet another embodiment, the compound of Formula I is
administered after the mammal is treated with the cytotoxic agent,
immunosuppressant, or radiation.
[0024] In another aspect of the invention, a method is provided for
allowing increased therapeutic irradiation dosage of a radiotherapy
or of a chemotherapy of a mammal, comprising: administering to the
mammal a compound of Formula I or a pharmaceutically acceptable
salt thereof,
##STR00002##
wherein, R.sub.1 is methyl, ethyl or trifluoromethyl at position 3,
4, 5 or 6; and R.sub.2 is hydroxyl, sulfydryl, C.sub.1-6 alkoxyl
such as methoxyl and ethoxyl, or a C.sub.1-6 alkylthio group such
as a methylthio group or an ethylthio group at position 2, 3 or 4;
or, R.sub.2 is absent.
[0025] In a preferred embodiment, the radiation or chemo therapy is
for the treatment of cancer or tumor, such as lung caner, breast
cancer, esophageal carcinoma, malignant lymphoma, and other
thoracic malignant tumors of the mammal.
[0026] In a preferred embodiment, the compound is pirfenidone.
[0027] In yet another aspect, the present invention provides a
method for preventing lung injury induced by radiation therapy or
chemotherapy of a mammal, comprising the steps of: before a
radiotherapy or a chemotherapy, administering to said mammal a
prophylactically effective amount of a compound of Formula I or a
pharmaceutically acceptable salt thereof,
##STR00003##
wherein R.sub.1 is methyl, ethyl or trifluoromethyl at position 3,
4, 5 or 6; and R.sub.2 is hydroxyl, sulfydryl, C.sub.1-6 alkoxyl
such as methoxyl and ethoxyl, or a C.sub.1-6 alkylthio group such
as a methylthio group or an ethylthio group at position 2, 3 or 4;
or, R.sub.2 is absent.
[0028] In a preferred embodiment, the radiation or chemo therapy is
for the treatment of cancer or tumor, such as lung caner, breast
cancer, esophageal carcinoma, malignant lymphoma, and other
thoracic malignant tumors of the mammal.
[0029] In yet another aspect, the present invention provides a
method for increasing the therapeutic irradiation dosage or the
therapeutic dosage of chemotherapy for a mammal, comprising the
steps of: (a) administering to said mammal in need of a
radiotherapy or a chemotherapy a compound of formula I or a
pharmaceutically acceptable salt thereof,
##STR00004##
wherein R.sub.1 is methyl, ethyl or trifluoromethyl at position 3,
4, 5 or 6; and R.sub.2 is hydroxyl, sulfydryl, C.sub.1-6 alkoxyl
such as methoxyl and ethoxyl, or a C.sub.1-6 alkylthio group such
as a methylthio group or an ethylthio group at position 2, 3 or 4;
or, R.sub.2 is absent, wherein, there is an original therapeutic
irradiation dose or chemotherapeutic dose set to be D.sub.0 for
said mammal; and (b) subjecting said mammal to a radiotherapy at an
irradiation dose or chemotherapeutic dose of D.sub.1, wherein
D.sub.1>D.sub.0.
[0030] In a preferred embodiment, said irradiation dose or
chemotherapeutic dose D.sub.1 and D.sub.0 fit the following
equation:
(D.sub.1-D.sub.0)/D.sub.0.gtoreq.20%.
[0031] In a preferred embodiment, the compound is Pirfenidone.
[0032] In a preferred embodiment, the radiation or chemo therapy is
for the treatment of cancer or tumor, such as lung caner, breast
cancer, esophageal carcinoma, malignant lymphoma, and other
thoracic malignant tumors of the mammal.
[0033] In a preferred embodiment, the compound of formula I is
administered at about 1 to 10 days (such as 2, 3, 4, 5, 6, 7, 8, or
9 days) before the radiotherapy or chemotherapy.
[0034] In an embodiment, the dose of the compound of formula I
administered is 0.1-1000 mg/kg, 0.5-500 mg/kg, 1-200 mg/kg, or
10-100 mg/kg body weight per day.
BRIEF DESCRIPTION OF THE DRAWINGS
[0035] FIG. 1 shows effects of F351 on reduction of renal fibrosis
induced by Adriamycin via pathological examination. Panel A:
Adriamycin-treated; and Panel B: Adriamycin+F351-treated.
[0036] FIG. 2 shows effects of F351 on reduction of lung fibrosis
induced by bleomycin via pathological examination with HE staining
(panels A-D, A: Control; B: Bleomycin-treated; C:
Bleomycin+F351-treated; D: Bleomycin+dexamethasone-treated); and
Masson staining (panels E-H, E: Control; F: Bleomycin-treated; G:
Bleomycin+F351-treated; H: Bleomycin+dexamethasone-treated).
[0037] FIG. 3 shows effects of F351 on reduction of kidney fibrosis
induced by cyclosporine A (CsA) via pathological examination with
Masson Staining Panel A: Control; Panel B: CsA-treated; and Panel
C: CsA+F351 -treated.
[0038] FIG. 4 shows effects of F351 on reduction of kidney fibrosis
induced by Adriamycin via pathological examination with Masson
Staining Panel A: Adriamycin-treated; and Panel B:
Adriamycin+F351-treated.
[0039] FIG. 5 is a graph showing adverse effects of dexamethasone
on the weight of rats as compared with rats treated with
pirfenidone.
DETAILED DESCRIPTION OF THE INVENTION
[0040] The present invention provides innovative compositions, kits
and methods for preventing, alleviating or treating of damages to
tissues or organs induced by various cytotoxic agents, such as
chemotherapeutic agents, biologics, immunosuppressants and
radiation. Inventors found that pyridone derivatives provided in
the present invention are able to prevent or reduce injuries to a
mammal who is treated with chemotherapy, an immunosuppressant or
radiation therapy. Thus, such prophylactic and/or therapeutic
effects of the pyridone derivatives make it possible to increase
therapeutic dosages of the cytotoxic agent, thereby enhancing the
therapeutic efficacy of the cytotoxic agent.
[0041] As exemplified in the Example section, animals treated with
chemotherapeutic agents (e.g., bleomycin and Adriamycin (i.e.,
doxorubicin), an immunosuppressant (e.g., cyclosporine) and
radiation suffered from extensive damages in the kidney and lung.
In contrast, when treated with the pyridone derivatives (e.g.,
pirfenidone and F351), such damages to the animal were
significantly reduced, marked by reduced mortality rates, and
reduced fibrosis in the kidney and lung. Compared with a
conventional anti-inflammatory agent, dexamethasone, the pyridone
derivatives provided in the present invention are not only safer
but also efficacious in protecting the animals from the cytotoxic
side effects of the drugs and radiation. Thus, the present
inventions are particularly useful in treatment of various diseases
involving the use of cytotoxic drugs or radiation.
[0042] In one aspect of the invention, the present invention
provides a method for preventing, treating or reducing damage to a
tissue or organ of a mammal who has been, is being, or will be
treated with a cytotoxic agent, an immunosuppressant, and/or
radiation, comprising: administering to the mammal a
therapeutically effective amount of a compound of Formula I or a
pharmaceutically acceptable salt thereof,
##STR00005##
wherein R.sub.1 is methyl, ethyl or trifluoromethyl at position 3,
4, 5 or 6; and R.sub.2 is hydroxyl, sulfydryl, C.sub.1-6 alkoxyl
such as methoxyl and ethoxyl, or a C.sub.1-6 alkylthio group such
as a methylthio group or an ethylthio group at position 2, 3 or 4;
or, R.sub.2 is absent.
[0043] In a preferred embodiment, the compound is pirfenidone.
[0044] In another preferred embodiment, the compound is
5-methyl-1-(4-hydroxylphenyl)-2-(1H)-pyridone, which is called
F351.
[0045] According to the method, the cytotoxic agent may be a
chemotherapeutic agent.
[0046] As used herein the term "chemotherapeutic agent" or
"chemotherapeutic" (or "chemotherapy", in the case of treatment
with a chemotherapeutic agent) is meant to encompass any
non-proteinaceous (i.e., non-peptidic) chemical compound useful in
the treatment of cancer, tumors, or other diseases. Examples of
chemotherapeutic agents include alkylating agents such as thiotepa
and cyclophosphamide (CYTOXAN.TM.); alkyl sulfonates such as
busulfan, improsulfan and piposulfan; aziridines such as benzodopa,
carboquone, meturedopa, and uredopa; ethylenimines and
methylamelamines including altretamine, triethylenemelamine,
triethylenephosphoramide, triethylenethiophosphoramide and
trimethylolomelamine; acetogenins (especially bullatacin and
bullatacinone); a camptothecin (including synthetic analogue
topotecan); bryostatin; callystatin; CC-1065 (including its
adozelesin, carzelesin and bizelesin synthetic analogues);
cryptophycins (articularly cryptophycin 1 and cryptophycin 8);
dolastatin; duocarmycin (including the synthetic analogues, KW-2189
and CBI-TMI); eleutherobin; pancratistatin; a sarcodictyin;
spongistatin; nitrogen mustards such as chlorambucil,
chlornaphazine, cholophosphamide, estramustine, ifosfamide,
mechlorethamine, mechlorethamine oxide hydrochloride, melphalan,
novembichin, phenesterine, prednimustine, trofosfamide, uracil
mustard; nitrosoureas such as carmustine, chlorozotocin,
foremustine, lomustine, nimustine, ranimustine; antibiotics such as
the enediyne antibiotics (e.g. calicheamicin, especially
calicheamicin gamma1I and calicheamicin phiI1, see, e.g., Agnew,
Chem. Intl. Ed. Engl., 33: 183-186 (1994); dynemicin, including
dynemicin A; bisphosphonates, such as clodronate; an esperamicin;
as well as neocarzinostatin chromophore and related chromoprotein
enediyne antibiotic chromomophores), aclacinomysins, actinomycin,
authramycin, azaserine, bleomycins, cactinomycin, carabicin,
carminomycin, carzinophilin, chromomycins, dactinomycin,
daunorubicin, detorubicin, 6-diazo-5-oxo-L-norleucine, doxorubincin
(Adriamycin.TM.) (including morpholino-doxorubicin,
cyanomorpholino-doxorubicin, 2-pyrrolino-doxorubicin and
deoxydoxorubicin), epirubicin, esorubicin, idarubicin,
marcellomycin, mitomycins such as mitomycin C, mycophenolic acid,
nogalamycin, olivomycins, peplomycin, potfiromycin, puromycin,
quelamycin, rodorubicin, streptonigrin, streptozocin, tubercidin,
ubenimex, zinostatin, zorubicin; anti-metabolites such as
methotrexate and 5-fluorouracil (5-FU); folic acid analogues such
as demopterin, methotrexate, pteropterin, trimetrexate; purine
analogs such as fludarabine, 6-mercaptopurine, thiamiprine,
thioguanine; pyrimidine analogues such as ancitabine, azacitidine,
6-azauridine, carmofur, cytarabine, dideoxyuridine, doxifluridine,
enocitabine, floxuridine; androgens such as calusterone,
dromostanolone propionate, epitiostanol, mepitiostane,
testolactone; anti-adrenals such as aminoglutethimide, mitotane,
trilostane; folic acid replinisher such as frolinic acid;
aceglatone; aldophosphamide glycoside; aminolevulinic acid;
eniluracil; amsacrine; bestrabucil; bisantrene; edatraxate;
defofamine; demecolcine; diaziquone; elfornithine; elliptinium
acetate; an epothilone; etoglucid; gallium nitrate; hydroxyurea;
lentinan; lonidamine; maytansinoids such as maytansine and
ansamitocins; mitoguazone; mitoxantrone; mopidamol; nitracrine;
pentostatin; phenamet; pirarubicin; losoxantrone; podophyllinic
acid; 2-ethylhydrazide; procarbazine; PSK.TM.; razoxane; rhizoxin;
sizofiran; spirogermanium; tenuazonic acid; triaziquone;
2,2',2''-trichlorotriethylamine; trichothecenes (especially T-2
toxin, verracurin A, roridin A and anguidine); urethane; vindesine;
dacarbazine; mannomustine; mitobronitol; mitolactol; pipobroman;
gacytosine; arabinoside ("Ara-C"); cyclophosphamide; thiopeta;
taxoids, e.g. paclitaxel (TAXOL.TM., Bristol Meyers Squibb
Oncology, Princeton, N.J.) and docetaxel (TAXOTERE.TM.,
Rhone-Poulenc Rorer, Antony, France); chlorambucil; gemcitabine
(Gemzar.TM.); 6-thioguanine; mercaptopurine; methotrexate; platinum
analogs such as cisplatin and carboplatin; vinblastine; platinum;
etoposide (VP-16); ifosfamide; mitroxantrone; vancristine;
vinorelbine (Navelbine.TM.); novantrone; teniposide; edatrexate;
daunomycin; aminopterin; xeoloda; ibandronate; CPT-11;
topoisomerase inhibitor RFS 2000; difluromethylornithine (DMFO);
retinoids such as retinoic acid; capecitabine; and pharmaceutically
acceptable salts, acids or derivatives of any of the above. Also
included in the definition of "chemotherapeutic agent" are
anti-hormonal agents that act to regulate or inhibit hormone action
on tumors such as anti-estrogens and selective estrogen receptor
modulators (SERMs), including, for example, tamoxifen (including
Nolvadex.TM.), raloxifene, droloxifene, 4-hydroxytamoxifen,
trioxifene, keoxifene, LY 117018, onapristone, and toremifene
(Fareston.TM.); inhibitors of the enzyme aromatase, which regulates
estrogen production in the adrenal glands, such as, for example,
4(5)-imidazoles, aminoglutethimide, megestrol acetate (Megace.TM.),
exemestane, formestane, fadrozole, vorozole (Rivisor.TM.),
letrozole (Femara.TM.), and anastrozole (Arimidex.TM.); and
anti-androgens such as flutamide, nilutamide, bicalutamide,
leuprolide, and goserelin; and pharmaceutically acceptable salts,
acids or derivatives of any of the above.
[0047] The cytotoxic agent may also be a tyrosine kinase inhibitor
such as ZD1839 (Iressa.TM. of AstraZeneca K.K.); IMC-C225 or
cetuximab (Erbitux.TM.); imatinib mesylate (GLEEVEC.TM., formerly
STI-571); and Sorafenib (Nexavar.TM.)
[0048] The biologic may be a therapeutic antibody such as
trastuzumab (HERCEPTIN.TM.); Rituximab (RITUXAN.TM.); alemtuzumab
(CAMPATH.TM.); and Gemtuzumab zogamicin (MYLOTARG.TM.).
[0049] The biologic may also be a cytokine such as the interleukins
and tumor-necrotic factors (TNFs).
[0050] Examples of the immunosuppressant include, but are not
limited to, Cyclosporins (Neoral, Sandimmune, SangCya);
Azathioprines (Imuran); corticosteroids such as prednisolone
(Deltasone, Orasone); basiliximab (Simulect); daclizumab (Zenapax);
muromonab CD3 (Orthoclone OKT3); tacrolimus (Prograf); Glatiramer
acetate (Copaxone); Mycopehnolate (CellCept) and Sirolimus
(Rapamune).
[0051] The radiation may be ionizing radiation.
[0052] The mammal may be a rodent such as a mouse or rat, but
preferably a human, more preferably a human patient having cancer,
tumor or in need of a tissue or organ transplant.
[0053] Specific types of cancers or malignant tumors, either
primary or secondary, that can be treated using this invention
include breast cancer, skin cancer, bone cancer, prostate cancer,
liver cancer, lung cancer, brain cancer, cancer of the larynx, gall
bladder, pancreas, rectum, parathyroid, thyroid, adrenal, neural
tissue, head and neck, colon, stomach, bronchi, kidneys, basal cell
carcinoma, squamous cell carcinoma of both ulcerating and papillary
type, metastatic skin carcinoma, osteo sarcoma, Ewing's sarcoma,
veticulum cell sarcoma, myeloma, giant cell tumor, small-cell lung
tumor, gallstones, islet cell tumor, primary brain tumor, acute and
chronic lymphocytic and granulocytic tumors, hairy-cell tumor,
adenoma, hyperplasia, medullary carcinoma, pheochromocytoma,
mucosal neuronms, intestinal ganglloneuromas, hyperplastic corneal
nerve tumor, marfanoid habitus tumor, Wilm's tumor, seminoma,
ovarian tumor, leiomyomater tumor, cervical dysplasia and in situ
carcinoma, neuroblastoma, retinoblastoma, soft tissue sarcoma,
malignant carcinoid, topical skin lesion, mycosis fungoide,
rhabdomyosarcoma, Kaposi's sarcoma, osteogenic and other sarcoma,
malignant hypercalcemia, renal cell tumor, polycythermia vera,
adenocarcinoma, glioblastoma multiforma, leukemias, lymphomas,
malignant melanomas, epidermoid carcinomas, and other carcinomas
and sarcomas.
[0054] In one embodiment, the compound of formula I is administered
prior to the treatment of the cytotoxic agent, immunosuppressant,
or radiation.
[0055] As used herein, the terms "the compound of the present
invention", "(a) pyridone derivative(s)", "a compound of formula
I", which are used interchangeably, refer to a compound having
structural formula I or a pharmaceutical acceptable salt
thereof,
##STR00006##
wherein R.sub.1 is methyl, ethyl or trifluoromethyl at position 3,
4, 5 or 6; and R.sub.2 is hydroxyl, sulfydryl, C.sub.1-6 alkoxyl
such as methoxyl, and ethoxyl, or a C.sub.1-6 alkylthio group such
as a methylthio group or an ethylthio group at position 2, 3 or 4;
or, R.sub.2 is absent.
[0056] In a preferred embodiment, R.sub.1 is methyl, and R.sub.2 is
absent. In a further preferred embodiment, R.sub.1 is methyl, and
R.sub.2 is hydroxyl. More preferably, R.sub.1 is methyl at position
5, and R.sub.2 is hydroxyl at position 4.
[0057] A particularly preferred compound is Pirfenidone.
[0058] Another particularly preferred compound is
5-methyl-1-(4-hydroxylphenyl)-2-(1H)-pyridone, which is called F351
having the structure shown below.
##STR00007##
[0059] The compounds of the present invention may also be in the
form of a salt formed with a pharmaceutically or physiologically
acceptable acid or base. Such salts include (but not limited to)
the salts formed with an inorganic acid selected from the group
consisting of hydrochloric acid, sulphuric acid, nitric acid and
phosphoric acid or an organic acid selected from the group
consisting of acetic acid, ethanedioic acid, butanedioic acid,
tartaric acid, methane sulfonic acid and maleic acid. Also, the
salts include those formed with an alkali metal or an alkaline
earth metal such as sodium, potassium, calcium or magnesium, and
those in the form of so-called "pro-drugs", which can be
transformed into active ingredients in vivo, including but not
limited to esters and carbamates.
[0060] The present invention also encompasses a pharmaceutical
composition and a therapeutic method. The said method comprises a
step of administration of a pharmaceutically effective amount of a
compound of formula Ito a mammal.
[0061] When the compound of the present invention is used in the
above-mentioned application, it can be mixed with one or more
pharmaceutically acceptable carriers or excipients such as
solvents, diluents, etc., and can be orally administered in a
dosage form selected from: tablets, pellets, capsules, dispensable
powder, particles or suspension (containing, e.g. about 0.05-5%
suspending agent), syrup (containing, e.g. about 10-50%
saccharide), and elixirs (containing, about 20-50% ethanol),
topically administered in the form of ointment, gel, medicated
bandage or patch, etc, or administered parenterally in the form of
a sterile injectable solution or suspension (containing about
0.05-5% suspending agent in an isotonic medium). For example, a
pharmaceutical preparation of the invention may contain, on a
weight basis, about 0.01-99%, preferably about 0.1%-90% of active
ingredients mixed with one or more carriers.
[0062] The effective dose of the active ingredient may vary with
the compound used, the administration mode and the severity of the
disease to be treated. However, typically, a desirable result can
be obtained when the compound of the present invention is
administered at about 0.25-1000 mg/kg body weight per day. More
preferably, the amount is separated into 2-4 doses per day, or is
delivered via a sustained-release form. For most of the large
mammals, the daily dose is about 1-100 mg/kg, more preferably about
2-80 mg/kg. The dosage form suitable for oral administration
comprises about 0.25-500 mg of the active compound homogeneously
mixed in a solid or liquid pharmaceutically acceptable carrier.
This dosing regime may be adjusted to obtain the best therapeutic
response. For example, depending on the urgency of the condition to
be treated, several separate doses may be administered daily, or
the dosage may be reduced in proportion.
[0063] The active compounds may be administered orally,
intravenously, intramuscularly or subcutaneously. Solid carriers
include starch, lactin, dicalcium phosphate, microcrystalline
cellulose, sucrose and white bole, and liquid carriers include
sterile water, polyethylene glycol, nonionic surfactant and edible
oil (e.g. corn oil, peanut oil and sesame oil), as long as these
carriers are compatible with the active ingredients and the desired
mode of administration. Advantageously, the adjuvants commonly used
in the production of a pharmaceutical composition, for example, a
flavoring agent, colorant, preservative and antioxidant such as
vitamin E, vitamin C, BHT and BHA, are also included.
[0064] To ease the production and the administration, the
pharmaceutical composition is preferably a solid one, especially in
the form of tablets and capsules filled with solid or liquid. Oral
administration of the compound is preferred.
[0065] The active compounds may be administered parenterally or
intraperitoneally. The active compounds (as free bases or
pharmaceutically acceptable salts) may be formulated into aqueous
solutions or suspensions mixed with surfactant (e.g. hydroxypropyl
cellulose, polyvinyl pyrrolidone) as desired. Dispersions can also
be prepared by mixing the active compounds in glycerin, liquid,
polyethylene glycol and/or oil. For the storage and usage under
normal condition, the preparations may contain preservatives to
prevent the growth of microorganisms.
[0066] The injectable forms include sterile aqueous solutions and
dispersions and sterile powder (freshly formulated into sterile
injection solutions or dispersions before use). Whatever, the
formulations should be sterile, and have sufficient fluidity to
allow the discharge from the injection devices such as a syringe.
The formulations should be stable under the condition of production
and storage, and capable of preventing contamination by
microorganisms (such as bacteria and fungi). The carrier may be a
solvent or a dispersant, including, for example, water, alcohols
(such as glycerin, propylene glycol and liquid polyethylene
glycol), the mixture thereof, and plant oil.
[0067] Additionally, the compound of the present invention may be
used in combination with one or more additional agents such as
.alpha.-interferon, .beta.-interferon, .gamma.-interferon, cortical
hormone and methotrexate.
[0068] When used for prophylaxis (or treatment) of acute lung
injuries, the pharmaceutical composition of the invention comprises
a pharmaceutically acceptable vehicle and a compound of formula I
(e.g. Pirfenidone), wherein the content of the compound of formula
I is in the range of 0.01% to 99.99% by weight, preferably 0.1% to
95% by weight, more preferably 0.5% to 90% by weight. Additionally,
the pharmaceutical composition may comprises an agent selected from
the group consisting of: a protease inhibitor, for example,
Sivelestat Sodium, anti-inflammatory agents, for example,
Ibuprofen, interferons and other non-steroid anti-inflammatory
drugs, phosphodiesterase inhibitor, for example, Pentoxifylline,
cytoprotector Amifostine, antioxidants N-acetylcysteine,
anti-endotoxin monoclonal antibody against inflammatory cells, the
medim thereof, and some pathogenic factors, IL-1 receptor
antigonist (IL-1 ra), TGF and its receptor antigonist, TGF .alpha.
and its receptor antigonist, and EGF/PDGF and its receptor
antigonist. It can also comprise a herb or its extract selected
from the following: salvia, astragalus, turtle shell or the mixture
thereof.
[0069] The dosage form of the present pharmaceutical composition is
not specifically limited. It may take the form of solid, semi-solid
or liquid. The pharmaceutical formulation according to the present
invention may be administered intraveinously, subcutaneouly, or via
other suitable administration routes. Oral administration is
preferred to give the desired effect. Also preferred is parenteral
administration, such as intravenous injection, which can reduce the
delay before the medicament takes effect in vivo.
[0070] The subject which can be treated with the present
pharmaceutical composition and by the method is mammals, in
particular human beings.
[0071] Although the effective therapeutic amounts of Pirfenidone
will vary with the ages and the conditions of the patient to be
treated, the prophylactic amount is generally from about 0.1 to 500
mg/kg, preferably from 0.1 to 200 mg/kg, more preferably from 0.5
to 100 mg/kg, still more preferably from 1 to 80 mg/kg. The
frequency of administration may be once or more than once a
day.
[0072] The present invention provides a method for preventing
radiation-induced lung injuries, comprising administering a
prophylactically effective amount of Pirfenidone to a patient in
need of a radiation therapy before the radiation therapy.
[0073] The present invention also provides a method to allow an
increased therapeutic irradiation dosage, comprising administering
a prophylactically effective amount of Pirfenidone to a patient to
be treated at an original irradiation dose of D.sub.0, and then
subjecting the patient to a radiation therapy at a irradiation dose
of D.sub.1, wherein D.sub.1>D.sub.0, preferably D.sub.1 is
higher than D.sub.0 by 20%, more preferably by 50%.
[0074] As used herein, the term "prophylactically effective amount"
refers to an administration amount which is capable of preventing
the radiation-induced lung injuries. Typically, this
prophylactically effective amount is from 1 to 50 mg/kg body
weight/day.
[0075] According to the present invention, Pirfenidone can be used
alone or in combination with pro-drugs.
[0076] The main advantages of the present invention lie in:
[0077] (i) Pirfenidone has remarkable prophylactic effect on
radiation-induced lung injuries with high safety and few toxic
side-effects;
[0078] (ii) Pirfenidone treatment allows a significantly increased
therapeutic irradiation dose.
[0079] The present invention will be further illustrated with the
following examples. It should be understood that, these examples
are exemplary only and are not intended to limit the scope of the
present invention. The experimental methods in the following
examples not indicating the specific experimental conditions are
typically carried out under the conventional conditions, or
following the manufacture's instructions.
EXAMPLES
Example 1
I. The Effects of Pirfenidone on Radiation-Induced Lung
Injuries
[0080] (a) Irradiation Method
[0081] Irradiation source: Varian 600C linear accelerator, 6MV X
ray
[0082] Irradiation method: The mice, six per row, were each
detained in an organic-glass cage. The cage was lid with a 1.5 cm
thick organic glass plate to compensate the buildup effect. The
source-to-skin distance was 100 cm, the irradiation field was
2.5.times.18 cm. The animal's head and abdomen were sheltered by
the lead baffle of the collimator, and thus only the chest was
exposed to the irradiation. The single dose of irradiation is 1200
cGy. A sealed transfer box equipped with an air filtering device
should be used to transfer the animals from or back to the animal
house.
[0083] Animals: 10-week old BALB/C mice
[0084] (b) Grouping of Animals:
[0085] Control Model group (60 mice): The mice were irradiated but
not treated with the compound.
[0086] Prophylaxis group (60 mice): The medication at a
predetermined dose was started 2 days before the irradiation. The
irradiation method and the dosage were the same as in the control
group. Pirfenidone was administered by gauvage (200 mg/kg,
dissolved in 0.5% CMC-Na and administrated at the volume of 0.1
ml/10 g) once a day for 3 months.
[0087] Treatment group (60 mice): The medication was started at day
0 of the irradiation. The irradiation method and dosage were the
same as in the control group. Pirfenidone was administered by
gauvage (200 mg/kg, dissolved in 0.5% CMC-Na and administrated at
the volume of 0.1 ml/10 g) once a day for 3 months.
[0088] Blank control group (60 mice): The mice were not irradiated,
and were only administered by gauvage with 0.5% CMC-Na once a day
for 3 months.
[0089] The mice of each group were observed once a day after
irradiation to examine the mortality. The cytometry and cell
sorting of the lavage, collagen content measurement
(hydroxyproline), pathomorphological observation were conducted
periodically after medicament treatment.
[0090] Cytometry of the bronchial alveolar lavage liquid: the right
lung was perfused with normal saline. 1.5-2.0 ml lavage liquid was
collected into an Ependoff tube by gentle suction. The content of
the inflammatory cells was counted manually by use of a
haemocytometer immediately after collection of the bronchial
alveolar lavage liquid.
[0091] Measurement of the hydroxyproline content: The measurement
was performed with isolated lung tissue by following the
instruction in the hydroxyproline kit.
[0092] Histopathologic examination: The isolated lung tissue was
fixed in formaldehyde solution, pafarrin-imbeded after dehydration,
and then subjected to HE staining and Masson trichrome staining
Evaluation criteria: Radiation-induced lung injuries was evaluated
according to the standard semi-quantitative scoreing of the
Ashcroft interstitial pulmonary fibrosis.
II. Results
[0093] The experiments showed that prophylactic and therapeutic
administration of Pirfenidone can both reduce the amount of
inflammatory cells in the bronchial alveolar lavage liquid, which
suggested that Pirfenidone has an inhibitory effect on pulmonary
inflammation response induced by radioactive irradiation. The
prophylactic administration showed more significant effect than the
therapeutic administration (FIG. 1 and Table 1).
TABLE-US-00001 TABLE 1 CELL COUNT OF BRONCHIAL ALVEOLAR LAVAGE
LIQUID Month 2 Month 3 Month 4 Month 5 Month 6 Blank 1.09 0.31 0.75
2.02 0.87 control Model 5.16 4.72 18.51 60.61 46.21 control
Treatment 5.78 1.04 8.04 45.55 39.29 group Prophylaxis 2.27 2.03
4.5 27.7 15 group
[0094] The experiments also showed that Pirfenidone could obviously
reduce the content of hydroxyproline (i.e. the amount of collagen
synthesis) in lung tissue, which suggested that Pirfenidone has an
inhibitory effect on fibrosis lesion induced by the radiation.
Surprisingly, the prophylactic administration showed more
significant effect than the therapeutic administration. The
difference was particularly significant in Month 5 and Month 6
(FIG. 2 and Table 2). This suggests that prophylactic
administration is more beneficial.
TABLE-US-00002 TABLE 2 MEASUREMENT OF HYDROXYPROLINE IN THE WHOLE
LUNG Month 1 Month 2 Month 3 Month 4 Month 5 Month 6 Blank 40.75
50.81 54.77 52.51 55.67 52.74 control Model 40.29 46.87 54.8 64
69.97 79.51 control Treatment 36.11 46.36 52.13 60.38 67.92 68.24
group Prophylaxis 43.85 43.81 50.46 56.98 58.1 58.15 group
Example 2
I. Pirfenidone can Increase the Tolerance to the Therapeutic
Irradiation Dosage
[0095] (a) method: the same as described in Example 1.
[0096] Prophylaxis group (60 mice): The medication was started 2
days before the irradiation. The mice were divided into two groups.
The animals were administered by gauvage with Pirfenidone (200
mg/kg, dissolved in 0.5% CMC-Na and administrated at the volume of
0.1 ml/10 g) once a day for 3 months.
[0097] Each group consisted of 60 mice. The mice of each group were
observed once a day after irradiation to examine the dyspnea and
mortality. The lung weighing, cytometry and cell sorting of the
bronchial alveolar lavage liquid, collagen content measurement
(hydroxyproline), measurement of cytokines (ACE, TGF-.beta.,
TGF-.alpha., etc) in the plasma, and pathomorphological observation
were conducted periodically after the medicament treatment.
[0098] The results indicated:
[0099] When Pirfenidone was administered prophylactically, the cell
count in the bronchial alveolar lavage liquid was lower than that
of the control group. This suggests that administration of
Pirfenidone before irradiation allow an increased therapeutic
irradiation dose.
Example 3
[0100] Twelve healthy subjects were administered with Pirfenidone
capsule at the dose of 400 mg, once a day or three times a day. The
medication continued for 5 days. The blood level of the medicine
was measured every day. The results indicated that blood stasis was
achieved at day 3 of the continuous administration at 400 mg. This
suggests that prophylactic administration of Pirfenidone (for
example, 2 days before the irradiation) can achieve the blood
stasis in vivo which may improve the therapeutic efficacy.
Example 4
I. Preparation of the Pharmaceutical Composition Comprising
Pirfenidone
[0101] (a) Injectable Formulation
[0102] Table 3 below lists the ingredients of an exemplary
formulation.
TABLE-US-00003 TABLE 3 Pirfenidone/F351 20-100 mg Sodium chloride
1-5 mg Water for Injection to the volume of 10 ml
[0103] Pirfenidone and sodium chloride were weighted according to
the above formulation, and were formulated into a solution. The
solution was then filled into 10 ml vials, sterilized and
packaged.
[0104] (b) Oral Formulation: Capsules
[0105] Table 4 below lists the ingredients of an exemplary oral
formulation in a form of capsule.
TABLE-US-00004 TABLE 4 Pirfenidone/F351 50-500 mg
Hydroxypropylmethylcellulose 2-10 mg Starch 50-100 mg Lactose 2-10
mg
[0106] The above excipients for 1000 capsules were weighed,
grinded, sieved and mixed to be homogenous. Then, the mixture was
added into Pirfenidone in the way of incremental addition. The
mixture was grinded to be homogenous, and then sieved through 80
mesh before filled into capsule.
[0107] (c) Oral Formulation: Sustained-Release Tablets
[0108] Table 5 below lists the ingredients of an exemplary oral
formulation in a form of sustained-release tablet.
TABLE-US-00005 TABLE 5 Pirfenidone/F351 50-500 mg
Hydroxypropylmethylcellulose 20-100 mg Sodium alginate 50-100 mg
Lactose 100-200 mg Microcrystalline cellulose 50-200 mg Magnesium
stearate 2-10 mg
[0109] Pirfenidone, lactose, hydroxypropylmethylcellulose and
sodium alginate were respectively weighted according to the
formulation indicated above (for 1000 capsules), grinded to fine
powder, sieved through 80 mesh and admixed. Then, the mixture was
mixed uniformly with microcrystalline cellulose, and was wetted
with water, screened through a 16-18 mesh sieve, dried at
60.degree. C., granuled. Magnesium stearate was then added, and the
mixture was pressed into tablets.
[0110] (d) Oral Formulation also Containing Herb Medicine
[0111] Table 6 below lists the ingredients of an exemplary oral
formulation in a form of capsule.
TABLE-US-00006 TABLE 6 Pirfenidone/F351 50-500 mg Salvia (Dan Shen
root) extract 50 mg Hydroxypropylmethylcellulose 2-10 mg Starch
50-100 mg Lactose 2-10 mg
[0112] The above excipients for 1000 capsules were weighed,
grinded, sieved, and mixed to homogeneity. Then, the mixture was
added into Pirfenidone in the way of incremental addition. The
mixture was grinded thoroughly to be homogeneous, and then sieved
through 80 mesh before filled into capsule.
Example 5
I. Synthesis of a Pirfenidone Analog
[0113] The analog was obtained through the following reaction,
wherein R.sub.1 in formula I is methyl at position 4, R.sub.2 is
hydroxymethyl at position 4:
##STR00008##
[0114] 4-methylpyridin-2(1H)-one was dissolved in dichloromethane,
to which pyridine in a catalytic amount was added. The temperature
was controlled between 0 to 5.degree. C.
[0115] 1-bromo-4-methoxybenzene was slowly dropped into this
mixture and allowed to react at room temperature for 2 hours. The
reaction mixture was poured into ice water, extracted twice with
dichloromethane, and the organic layer was collected. The collected
organic layer was washed with saturated saline solution, dried over
sodium sulfate, and then concentrated to give a crude product. The
crude product was subject to column chromatography to give the
final product.
Example 6
I. Effect of 5-methylpyridin-1(4-hydroxyphenyl)-2-(1H)-one on
Radiation-Induced Lung Injuries
[0116] The experiments in Example 1 are repeated, except that
5-methylpyridin-1(4-hydroxyphenyl)-2-(1H)-one (F351) rather than
Pirfenidone is used, with twenty mice in each group.
[0117] The experiments should show that prophylactic and
therapeutic administration of compound F351 can both reduce the
amount of inflammatory cells in the bronchial alveolar lavage
liquid. And, the prophylactic administration may be more effective
than the therapeutic administration.
Discussion
[0118] Lung injuries is a common problem during radiotherapy of
thoracic tumors, which include two phases, early and late phases of
radiation-induced lung injuries. The early-phase lung injuries
(radiation pneumonitis) generally occurs during the course of
irradiation, leading to severe consequence including interruption
of the irradiation course and thus influencing the therapeutic
effect, or even leading to death of patients.
[0119] Pulmonary infection can also deteriorate fibrosis of the
lung. Late phase of the radiation-induced lung-injuries primarily
depends on prophylaxis. There is not currently available medicament
specifically for the treatment of radiation-induced lung-injuries,
and the prophylactic medicament for this disease has not been
reported yet.
[0120] Once the pulmonary fibrosis has occurred, the treatment will
be a complex and long-term course. Though Pirfenidone has excellent
clinically therapeutic effect on radiation-induced lung injuries,
it does not guarantee that the patient can be completely cured
without any sequelae. Consequently, prevention before the onset is
more practically important than treatment after onset of the
disease. Pirfenidone, a small compound, has the effect of
inhibiting tissue fibrosis, improving pulmonary function,
anti-inflammation, and anti-fibrosis. When being administered
before the formation of pulmonary fibrosis, Pirfenidone can inhibit
collagen synthesis, prevent fibroblast proliferation, and
down-regulate many cytokines, thereby preventing development of
radiation pneumonitis and radiation pulmonary fibrosis.
[0121] Radiation-induced lung injuries during radiotherapy can
hardly be avoided. Administration of Pirfenidone compounds before
radiotherapy can, on one hand, kill tumorous tissues, and on the
other hand, provide the best prevention against radiation-induced
lung injuries, and hence improve life quality of the patients,
thereby greatly enhancing the therapeutic effect on tumors.
Therefore, it has significant theoretic and clinical values.
Example 7
I. Treatment of Adriamycin-Induced Renal Toxicity Using F351
[0122] Methods:
[0123] 20 male Wistar rats (with body weight 180-220 g) were
randomly divided into three groups with 4 rats in control group, 8
rats in Adriamycin-treated group, and 8 rats in
Adriamycin+F351-treated group. All the animals (except for those in
control group) were injected with 7.5 mg/kg adriamycin by tail vein
injection on day 1 of the study. Animals in Adriamycin+F351-treated
group were administrated by gauvage with 250 mg/kg F351 per day for
12 weeks starting on day 1 of the study. Animals in the control
group were injected with PBS, instead. The study lasted for 12
weeks.
[0124] Observation:
[0125] Rat urine was collected 24 hours before the end of the study
and amount of urine proteins was determined. Renal tissues were
also collected for pathology examination.
[0126] Results:
[0127] As shown in Table 7, treatment of F351 reduced amount of
urine proteins significantly. We also observed that treatment of
F351 reduced the degree of kidney fibrosis by pathology
examination. As shown in FIG. 1, animals in Adriamycin-treated
group showed glomerular intumescence, increased mesangial cell
proliferation and mesangial matrix, focal segmental sclerosis,
hyperplasia of focal fibrous tissue in tubule, and increased
collagen deposition (Green color in masson staining indicated
collagen deposition). Animals in F351-treated group showed slight
glomerular intumescence, normal mesangial cell proliferation,
slight hyperplasia of focal fibrous tissue, decreased inflammatory
cell infiltration, and reduced collagen deposition.
TABLE-US-00007 TABLE 7 EFFECT OF F351 ON REDUCTION OF AMOUNT OF
URINE PROTEINS Amount of Urine Proteins Group (mg/24 hrs, x .+-. s)
Control 9.33 .+-. 0.62 Adriamycin-treated 89.13 .+-. 12.57
Adriamycin + F351-treated 54.32 .+-. 10.88
Example 8
I. Treatment of Bleomycin-Induced Lung Fibrosis Using F351
[0128] Methods:
[0129] Forty eight (48) C57B6 mice (with body weight 16-18 g), half
male and half female, were used in the study. Animals were divided
into four groups with 12 animals per group. All the animals (except
for those in control group) were injected with 5 mg/kg Bleomycin in
the volume of 50 ul by intratracheal instillation on day 1 of the
study. Animals in control group were injected by PBS instead.
Details of the drug administration are described in Table 8.
TABLE-US-00008 TABLE 8 DRUG ADMINISTRATION IN THE STUDY Group
Administration Control Administered by gauvage, 0.5% CMC-Na, 0.2
ml/day for 28 days Bleomycin-treated Bleomycin was administered at
5 mg/kg in the volume of 50 ul by intratracheal instillation on day
1 Bleomycin + Bleomycin was administered at 5 mg/kg
dexamethasone-treated in the volume of 50 ul by intratracheal
instillation on day 1; dexamethasone by gauvage at 3 mg/kg per day
in the volume of 0.2 ml for 28 days (dose was adjusted according to
the animal's response) Bleomycin + F351-treated Bleomycin was
administered at 5 mg/kg in the volume of 50 ul by intratracheal
instillation on day 1; F351 by gauvage at 100 mg/kg/day in the
volume of 0.2 ml for 28 days
[0130] Results:
[0131] Pathology examination was performed at day 28. Animals in
control group showed basically normal fibrotic tissues with slight
thickness of alveolar wall, hyperplasia of pulmonary interstitial,
and effusion of inflammatory cells. Fibrous tissue of animal in
control group was basically normal. Animals in Bleomycin-treated
group showed severe alveolar inflammation and hyperplasia of
fibrotic tissue, thickness of alveolar wall, significant pulmonary
interstitial hyperplasia, and an increased amount of effused
inflammatory cells. Animals in Bleomycin+F351-treated group showed
a significant improvement of the above pathological changes with
reduced fibrosis. Animals in Bleomycin+Dex-treated group showed a
slight improvement of the above pathological changes (FIG. 2).
[0132] Table 9 shows effects of F351 treatment on reduction of lung
index. Lung index of animals in Bleomycin-treated group was
significantly increased compared to those in control group. Lung
index of animals in Bleomycin+F351-treated group was decreased
compared to those in Bleomycin-treated group.
TABLE-US-00009 TABLE 9 EFFECT OF F351 TREATMENT ON REDUCTION OF
LUNG INDEX Group Lung Index (.+-.S) Control 807.12 .+-. 100.60
Bleomycin-treated 2033.00 .+-. 1176.01 Bleomycin + F351 (100
mg/kg)-treated 1511.71 .+-. 945.55 Bleomycin +
dexamethasone-treated 1083.90 .+-. 289.66
Example 9
I. Prevention and/or Treatment of Radiation-Induced Lung
Fibrosis
[0133] Methods:
[0134] BalB/C male mice were randomly divided into three groups,
namely, Control group (C group), Radiation group (R group), and
Pirfenidone+Radiation (P+R group). All the animals were given
single dose radiation on chest at 1200 cGy. Drugs were
administrated two days before radiation for 12 weeks at 200
mg/kg/day. Examination includes cytometry of macrophages (performed
4 weeks and 8 weeks after drug administration), determination of
hydroproline level (performed 16 weeks and 24 weeks after drug
administration), and pathology examination of lung tissues by HE
staining and Masson staining and evaluation of lung fibrosis by
Ashcroft semi-quantitative evaluation standard under a microscope
(performed 16 weeks and 24 weeks after drug administration).
[0135] Results:
[0136] Pirfenidone treatment had little toxic effect on BalB/C mice
and reduced lung fibrosis mainly by decreasing congregation of
macrophages in alveolus and collagen deposition in the lung. The
study indicates that pirfenidone has preventive effects on
radiation-induced lung fibrosis (Table 10).
TABLE-US-00010 TABLE 10 EFFECTS OF PIRFENIDONE ON PREVENTION AND/OR
TREATMENT OF RADIATION-INDUCED LUNG FIBROSIS A. Cytometry of
macrophages (1 .times. 10.sup.4/ml) Group 4 weeks 8 weeks C group
1.25 .+-. 1.37 1.09 .+-. 1.04 R group 9.17 .+-. 4.08 5.16 .+-. 2.16
P + R group 6.67 .+-. 2.04 2.27 .+-. 1.41 P 0.333 0.008 Group 16
weeks 24 weeks B. Evaluation on lung fibrosis C group 11.55 .+-.
3.72 25.56 .+-. 15.17 R group 47.91 .+-. 22.49 42.50 .+-. 17.24 P +
R group 29.15 .+-. 18.74 19.46 .+-. 6.67 P 0.039 0.000 C.
Hydroproline level (.mu.g/mg) C group 62.30 .+-. 13.92 80.91 .+-.
19.68 R group 86.05 .+-. 11.92 104.14 .+-. 20.99 P + R group 67.65
.+-. 11.99 79.15 .+-. 10.90 P 0.007 0.001
Example 10
I. Prophylactic and/or Therapeutic Use of F351 in Drug-Induced
Nephrotoxicity
[0137] 1. Introduction--Kidney Fibrosis
[0138] Kidney fibrosis, characterized by glomerulo-sclerosis and
tubulointerstitial fibrosis, is a pathological manifestation of
chronic kidney injury. Common insults inducing kidney fibrosis
include chronic infection, obstruction of the ureter by calculi,
malignant hypertension, severe diabetic conditions, and chronic
exposure to heavy metals such as lead, vanadium, and mercury, or
drugs such as diuretic, non-steroidal anti-inflammatory drug
(NSAID), immunosuppressants, anti-tumor drugs and anticoagulants.
In addition, idiopathic glomerulosclerosis and renal interstitial
fibrosis have been reported in children and adults. Kidney fibrosis
correlates well with the overall loss of renal function and
frequently contributes to end-stage life-threatening renal
failure.
[0139] 2. Introduction--Drug-Induced Nephrotoxicity
[0140] Cyclosporine A (CsA), a calcineurin inhibitor, has improved
allograft survival in solid organ transplantation and has been
increasingly applied in the treatment of various autoimmune
diseases. While marked progresses have been made in patients and
allograft survival rates, clinical use of CsA is often limited by
its nephrotoxic effects, which can be presented as two distinct and
well-characterized forms: acute and chronic nephrotoxicity. The
acute form is characterized by renal vasoconstriction, induced by
an imbalance of vasoactive substances release, which leads to renal
dysfunction. This form is reversible. The chronic toxicity, in
contrast, is characterized by the vasoconstriction plus the
development of structural damage that includes arteriolopathy and
tubulointerstitial fibrosis that are often not reversible. The
exact mechanisms of CsA-induced nephrotoxicity are not fully
understood and there is no effective prevention or treatment for
CsA-induced renal toxicity so far.
[0141] Adriamycin, a potent chemotherapeutic agent, is effective in
treatment of leukemias, lymphomas and of many solid tumors.
However, its clinical usage is often limited by toxic effects in
many organs, including cardio toxicity, which is induced by oxygen
radical damage of membrane lipids. In addition, Adriamycin causes
severe nephrotoxicity in a variety of experimental animals, which
represents a model very close to human progressive chronic renal
disease. The molecular mechanism by which Adriamycin causes renal
damage is unknown.
II. Treatment of Cyclosporine A-Induced Nephrotoxicity Using
F351
[0142] Methods:
[0143] 24 male Wistar rats (with body weight 180-220 g) were given
low salt diet for 7 days before the study and randomly divided into
three groups with 8 rats per group. The groups were named as
control group, cyclosporine A (CsA)-treated group, and
CsA+F351-treated group. The study was performed as shown in Table
11.
TABLE-US-00011 TABLE 11 DRUG ADMINISTRATION IN THE STUDY Group Drug
Administration Control Subcutaneous injection of olive oil
CsA-treated Subcutaneous injection of 15 mg/kg CsA dissolved in
olive oil CsA + F351-treated Subcutaneous injection of 15 mg/kg CsA
dissolved in olive oil; F351 administered at 100 mg/Kg/day
[0144] The animals were treated once a day for 28 days as indicated
in the above table. Once every 7 days, the animals were weighed and
the dose of CsA and F351 were adjusted in terms of body weight. Low
salt diet was given to the animals during the whole study
period.
[0145] Observation:
[0146] Mean arterial pressure was determined in alert state of the
animals at the end of the study. Renal tissues were also collected
for pathology examination.
[0147] Results:
[0148] As showed in Table 12, treatment of F351 reduced the mean
tail arterial pressure and the degree of kidney fibrosis
significantly.
TABLE-US-00012 TABLE 12 EFFECT OF F351 TREATMENT ON CsA-INDUCED
NEPHROTOXICITY Animal Mean Arterial Pressure Score on Kidney Group
No. (kPa, x .+-. s) Fibrosis Control 8 15.66 .+-. 0.38 0.18 .+-.
0.04 CsA-treated 7 17.74 .+-. 0.51 1.13 .+-. 0.31 CsA +
F351-treated 8 16.02 .+-. 0.27 0.62 .+-. 0.15
[0149] Pathology examination showed animals in CsA-treated group
had various degrees of pathological changes, including
tubulointerstitial fibrosis, tubular atrophy in the fibrotic area,
and thickness of basal lamina. Kidney fibrosis in F351-treated
animals was greatly reduced compared to animals in modeling group
(FIG. 3).
III. Treatment of Adriamycin-Induced Nephrotoxicity
[0150] Methods:
[0151] 20 male Wistar rats (with body weight 180-220 g) were
randomly divided into three groups with 4 rats in control group, 8
rats in Adriamycin-treated group, and 8 rats in
Adriamycin+F351-treated group. All the animals (except for those in
control group) were injected with 7.5 mg/kg adriamycin by tail vein
injection at day 1 of the study. Animals in control group were
injected with PBS instead. Animals in Adriamycin+F351-treated group
were administrated by gauvage with 250 mg/kg F351 per day for 12
weeks starting on day 1.
[0152] Observation:
[0153] Rat urine was collected 24 hours before the end of the study
and amount of urine proteins was determined. Renal tissues were
also collected for pathology examination.
[0154] Results:
[0155] As shown in Table 13, treatment of F351 reduced amount of
urine proteins significantly. It was also observed that treatment
of F351 reduced the degree of kidney fibrosis by pathology
examination. As shown in FIG. 4, animals in Adriamycin-treated
group showed glomerular intumescence, increased mesangial cell
proliferation and mesangial matrix, focal segmental sclerosis,
hyperplasia of focal fibrous tissue in tubule, and increased
collagen deposition (Green color in masson staining indicated
collagen deposition). Animals in F351-treated group showed slight
glomerular intumescence, normal mesangial cell proliferation,
slight hyperplasia of focal fibrous tissue, decreased inflammatory
cell infiltration, and reduced collagen deposition.
TABLE-US-00013 TABLE 13 EFFECT OF F351 ON REDUCTION OF AMOUNT OF
URINE PROTEINS Amount of Urine Proteins Group (mg/24 hrs, x .+-. s)
Control 9.33 .+-. 0.62 Adriamycin-treated 89.13 .+-. 12.57
Adriamycin + F351-treated 54.32 .+-. 10.88
Example 11
I. Side Effects of Dexamethasone Compared with Pirfenidone
[0156] 1. Introduction
[0157] Dexamethasone, a member of glucocorticoid hormones, has an
effect on anti-inflammation and immune-suppression. The mechanism
of action might involve inhibition of inflammatory cell
infiltration, reduction of immune complex, inhibition of macrophage
proliferation, and reduction of cytokine secretion by macrophage.
Clinical use of dexamethasone is limited by its side effects,
including depression, hypertension, nausea, acne, bone pain, muscle
wasting, and anorexia. The side effects of dexamethasone also
include risks of developing infection since dexamethasone
suppresses the inflammatory response and makes it more difficult
for a patient's body to fight off any infection.
[0158] 2. Pharmacological Assay
[0159] Dexamethasone treatment was used as positive control in one
study was designed to investigate the effects of pirfenidone on
bleomycin-induced lung fibrosis in rat. Loss of body weight and
high mortality rate were observed in dexamethasone-treated group
(FIG. 5 and Table 14), though pathological examination showed
dexamethasone treatment reduced lung fibrosis significantly.
Further examination revealed that animal death was caused by
toxicity effect induced by high dose of dexamethasone, rather than
pulmonary alveolitis or pulmonary interstitial fibrosis.
TABLE-US-00014 TABLE 14 HIGH MORTALITY RATE OBSERVED IN
DEXAMETHASONE-TREATED GROUP Number of Mortality Group dead animal
rate P value Control 1/10 10% 0.025 Bleomycin-treated 4/12 33.3%
0.005 Bleomycin + Pirfenidone- 0/10 0% treated Bleomycin +
Dexamethasone- 9/14 64.3% treated
[0160] All references mentioned in this application are herein
incorporated by reference into the specification to the same extent
as if each was specifically and individually indicated to be
incorporated herein by reference. Additionally, it will be
understood that in light of the above disclosure of the present
invention, those skilled in the art can make various changes and
modifications, all of which are falling in the scope of the claims
of the present invention.
* * * * *