U.S. patent application number 11/394279 was filed with the patent office on 2006-07-27 for randomized libraries of zinc finger proteins.
This patent application is currently assigned to Sangamo Biosciences, Inc.. Invention is credited to Casey C. Case, Andrew Jamieson, Edward J. Rebar.
Application Number | 20060166263 11/394279 |
Document ID | / |
Family ID | 23811426 |
Filed Date | 2006-07-27 |
United States Patent
Application |
20060166263 |
Kind Code |
A1 |
Case; Casey C. ; et
al. |
July 27, 2006 |
Randomized libraries of zinc finger proteins
Abstract
The present invention relates to methods of using libraries of
randomized zinc finger proteins to identify genes associated with
selected phenotypes.
Inventors: |
Case; Casey C.; (San Mateo,
CA) ; Rebar; Edward J.; (El Cerrito, CA) ;
Jamieson; Andrew; (San Francisco, CA) |
Correspondence
Address: |
ROBINS & PASTERNAK
1731 EMBARCADERO ROAD
SUITE 230
PALO ALTO
CA
94303
US
|
Assignee: |
Sangamo Biosciences, Inc.
Richmond
CA
|
Family ID: |
23811426 |
Appl. No.: |
11/394279 |
Filed: |
March 29, 2006 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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10337216 |
Jan 6, 2003 |
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11394279 |
Mar 29, 2006 |
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09731558 |
Dec 6, 2000 |
6503717 |
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10337216 |
Jan 6, 2003 |
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09456100 |
Dec 6, 1999 |
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09731558 |
Dec 6, 2000 |
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Current U.S.
Class: |
435/6.18 ;
506/9 |
Current CPC
Class: |
C12N 15/1079
20130101 |
Class at
Publication: |
435/006 |
International
Class: |
C40B 40/08 20060101
C40B040/08 |
Claims
1. A method for making a nucleic acid library encoding randomized
zinc finger proteins, the method comprising: a) providing a first
polynucleotide encoding a first zinc finger; and b) randomly
combining the first polynucleotide with a plurality of additional
polynucleotides, wherein at least one of the additional
polynucleotides encodes a zinc finger that is heterologous to the
first zinc finger.
2. The method of claim 1 wherein a plurality of first
polynucleotides are provided, each of which encodes a different
zinc finger.
3. The method of claim 1, wherein the library encodes three-finger
zinc finger proteins.
4. A method for making a nucleic acid library encoding randomized
zinc finger proteins, wherein each protein in the library comprises
n zinc fingers, the method comprising: a) providing q different
zinc finger sequences, wherein q is greater than n; and b) randomly
combining the q different zinc finger sequences.
Description
CROSS-REFERENCES TO RELATED APPLICATIONS
[0001] This application is a continuation of U.S. patent
application Ser. No. 10/337,216 filed Jan. 6, 2003, which is a
continuation of U.S. patent application Ser. No. 09/731,558 filed
Dec. 6, 2000, now U.S. Pat. No. 6,503,717, which is a
continuation-in-part of U.S. patent application Ser. No. 09/456,100
filed Dec. 6, 1999, now abandoned; the disclosures of which are
herein incorporated by reference in their entireties.
[0002] This application is related to U.S. Ser. No. 09/229,007,
filed Jan. 12, 1999, and U.S. Ser. No. 09/229,037, filed Jan. 12,
1999, and U.S. Ser. No. 09/395,448, filed Sep. 14, 1999, herein
each incorporated by reference in their entirety.
STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED
RESEARCH AND DEVELOPMENT
[0003] Not applicable.
FIELD OF THE INVENTION
[0004] The present invention relates to methods of using libraries
of randomized zinc finger proteins to identify genes associated
with selected phenotypes.
BACKGROUND OF THE INVENTION
[0005] A. Using Libraries to Identify Genes Associated with a
Selected Phenotype
[0006] Identification of gene function is a critical step in the
selection of new molecular targets for drug discovery, gene
therapy, clinical diagnostics, agrochemical discovery, engineering
of transgenic plants, e.g., with novel resistance traits or
enhanced nutritional characteristics, and genetic engineering of
prokaryotes and higher organisms for the production of industrial
chemicals, biochemicals, and chemical intermediates. Historically,
library screening methods have been used to screen large numbers of
uncharacterized genes to identify a gene or genes associated with a
particular phenotype, e.g., hybridization screening of nucleic acid
libraries, antibody screening of expression libraries, and
phenotypic screening of libraries.
[0007] For example, molecular markers that co-segregate with a
disease trait in a segment of patients can be used as nucleic acid
probes to identify, in a library, the gene associated with the
disease. In another method, differential gene expression in cells
and nucleic acid subtraction can be used to identify and clone
genes associated with a phenotype in the test cells, where the
control cells do not display the phenotype. However, these methods
are laborious because the screening step relies heavily on
conventional nucleic acid cloning and sequencing techniques.
Development of high throughput screening assays using these methods
would therefore be cumbersome.
[0008] An example of phenotypic screening of libraries is discovery
of transforming oncogenes (see, e.g., Goldfarb et al., Nature
296:404 (1982)). Oncogenic transformation can be observed in NIH
3T3 cells by assaying for loss of contact inhibition and foci
formation. cDNA expression libraries from transformed cells are
introduced into untransformed cells, and the cells were examined
for foci formation. The gene associated with transformation is
isolated by clonal propagation and rescue of the expression vector.
Unfortunately, this method is limited by phenotype and can only be
used to assay for transdominant genes.
[0009] Advances in the field of high throughput screening have
increased the cell types and phenotypes that can be investigated
using library screening methods. Viral vectors such as retroviral,
adenoviral, and adenoviral associated vectors have been developed
for efficient nucleic acid delivery to cells (see, e.g., U.S. Pat.
No. 5,173,414; Tratschin et al., Mol. Cell. Biol. 5:3251-3260
(1985); Tratschin, et al., Mol. Cell. Biol. 4:2072-2081 (1984);
Hermonat & Muzyczka, Proc. Nat'l Acad. Sci. USA 81:6466-6470
(1984); and Samulski et al., J. Virol. 63:03822-3828 (1989);
Buchscher et al., J. Virol. 66:2731-2739 (1992); Johann et al., J.
Virol. 66:1635-1640 (1992); Sommerfelt et al., Virol. 176:58-59
(1990); Wilson et al., J. Virol. 63:2374-2378 (1989); Miller et
al., J. Virol. 65:2220-2224 (1991); and PCT/US94/05700). Cells can
be phenotypically analyzed either one at a time, using flow
cytometry, or in arrayed clonal populations, using liquid handling
robots. These techniques allow a sufficient number of library
members to be tested for a wide range of potential phenotypes.
[0010] Currently, libraries of random molecules are being used with
phenotypic screening for the discovery of genes associated with a
particular phenotype. For example, random peptide or protein
expression libraries are being used to block specific
protein-protein interactions and produce a particular phenotype
(see, e.g., Caponigro et al., Proc. Nat'l Acad. Sci USA
95:7508-7513 (1998); WO 97/27213; and WO 97 27212). In another
method, random antisense nucleic acids or ribozymes are used to
inactivate a gene and produce a desired phenotype (see, e.g., WO
99/41371 and Hannon et al., Science 283:1125-1126 (1999)).
[0011] The main shortcoming of these methods is the inherent
inefficiency of the random molecules, which vastly increases the
size of the library to be screened. Even with a known target
nucleic acid or protein, literally hundreds of antisense, ribozyme,
or peptide molecules must be empirically tested before identifying
one that will inhibit gene expression or protein-protein
interactions. Since the random library must be enormous to produce
sufficient numbers of active molecules, huge numbers of cells must
be screened for phenotypic changes. For unknown gene and protein
targets, the rarity of effective, bioactive peptides, antisense
molecules, or ribozyme molecules imposes significant constraints on
high throughput screening assays. Furthermore, these methods can be
used only for inhibition of gene expression, but not for activation
of gene expression. This feature limits identification of gene
function to phenotypes present only in the absence of gene
expression.
[0012] Therefore, efficient high throughput library screening
methods allowing random inhibition or activation of uncharacterized
genes would be of great utility to the scientific community. These
methods would find widespread use in academic laboratories,
pharmaceutical companies, genomics companies, agricultural
companies, chemical companies, and in the biotechnology
industry.
[0013] B. Zinc Finger Proteins as Transcriptional Regulators
[0014] Zinc finger proteins ("ZFPs") are proteins that bind to DNA
in a sequence-specific manner and are typically involved in
transcription regulation. Zinc finger proteins are widespread in
eukaryotic cells. An exemplary motif characterizing one class of
these proteins (the Cys.sub.2His.sub.2 class) is
-Cys-(X).sub.2-4-Cys-(X).sub.12-His-(X).sub.3-5-His (where X is any
amino acid). A single finger domain is about 30 amino acids in
length and several structural studies have demonstrated that it
contains an alpha helix containing the two invariant histidine
residues co-ordinated through zinc with the two cysteines of a
single beta turn. To date, over 10,000 zinc finger sequences have
been identified in several thousand known or putative transcription
factors. Zinc finger proteins are involved not only in
DNA-recognition, but also in RNA binding and protein-protein
binding. Current estimates are that this class of molecules will
constitute the products of about 2% of all human genes.
[0015] The X-ray crystal structure of Zif268, a three-finger domain
from a murine transcription factor, has been solved in complex with
its cognate DNA-sequence and shows that each finger can be
superimposed on the next by a periodic rotation and translation of
the finger along the main DNA axis. The structure suggests that
each finger interacts independently with DNA over 3 base-pair
intervals, with side-chains at positions -1, 2, 3 and 6 on each
recognition helix making contacts with respective DNA triplet
sub-site.
[0016] The structure of the Zif268-DNA complex also suggested that
the DNA sequence specificity of a zinc finger protein could be
altered by making amino acid substitutions at the four helix
positions (-1, 2, 3 and 6) on a zinc finger recognition helix,
using, e.g., phage display experiments (see, e.g., Rebar et al.,
Science 263:671-673 (1994); Jamieson et al., Biochemistry
33:5689-5695 (1994); Choo et al., Proc. Natl. Acad. Sci. U.S.A.
91:11163-11167 (1994); Greisman & Pabo, Science 275:657-661
(1997)). For example, combinatorial libraries were constructed with
zinc finger proteins randomized in either the first or middle
finger. The randomized zinc finger proteins were then isolated with
altered target sites in which the appropriate DNA sub-site was
replaced by an altered DNA triplet. Correlation between the nature
of introduced mutations and the resulting alteration in binding
specificity gave rise to a set of substitution rules for rational
design of zinc finger proteins with altered binding specificity.
These experiments thus demonstrated that randomized zinc finger
proteins could be made, which demonstrated altered target sequence
specificity.
[0017] Recombinant zinc finger proteins, often combined with a
heterologous transcriptional activator or repressor domain, have
also shown efficient transcriptional regulation of transiently
expressed reporter genes in cultured cells (see, e.g., Pomerantz et
al., Science 267:93-96 (1995); Liu et al., Proc. Natl. Acad. Sci.
U.S.A. 94:5525-5530 1997); and Beerli et al., Proc. Natl. Acad.
Sci. U.S.A. 95:14628-14633 (1998)). For example, Pomerantz et al.,
Science 267:93-96 (1995) designed a novel DNA binding protein by
fusing two fingers from Zif268 with a homeodomain from Oct-1. The
hybrid protein was then fused with either a transcriptional
activator or repressor domain for expression as a chimeric protein.
The chimeric protein was reported to bind a target site
representing a hybrid of the subsites of its two components. The
chimeric DNA binding protein also activated or repressed expression
of a reporter luciferase gene having a target site.
[0018] Liu et al., Proc. Natl. Acad. Sci. U.S.A. 94:5525-5530
(1997) constructed a composite zinc finger protein by using a
peptide spacer to link two component zinc finger proteins, each
having three fingers. The composite protein was then further linked
to transcriptional activation or repression domains. The resulting
chimeric protein bound to a target site formed from the target
segments bound by the two component zinc finger proteins. The
chimeric zinc finger protein activated or repressed transcription
of a reporter gene having the target site.
[0019] Beerli et al., Proc. Natl. Acad. Sci. U.S.A. 95:14628-14633
(1998) constructed a chimeric six finger zinc finger protein fused
to either a KRAB, ERD, or SID transcriptional repressor domain, or
the VP16 or VP64 transcriptional activation domain. This chimeric
zinc finger protein was designed to recognize an 18 bp target site
in the 5' untranslated region of the human erbB-2 gene. This
construct both activated and repressed a transiently expressed
reporter luciferase construct linked to the erbB-2 promoter.
[0020] In addition, a recombinant zinc finger protein was reported
to repress expression of an integrated plasmid construct encoding a
bcr-abl oncogene (Choo et al., Nature 372:642-645 (1994)). Phage
display was used to select a variant zinc finger protein that bound
to the selected target segment. The variant zinc finger protein
thus isolated was then reported to repress expression of a stably
transfected bcr-abl construct in a cell line. To date, these zinc
finger protein methods have focused on regulation of either single,
transiently expressed, known genes, or on regulation of single,
known exogenous genes that have been integrated into the
genome.
SUMMARY OF THE INVENTION
[0021] The present application therefore provides for the first
time methods of using libraries of randomized zinc finger proteins
to screen large numbers of genes, for identifying a gene or genes
associated with a selected phenotype. These libraries of randomized
zinc finger DNA binding proteins have the ability to regulate gene
expression with high efficiency and specificity. Because zinc
finger proteins provide a reliable, efficient means for regulating
gene expression, the libraries of the invention typically have no
more than about 10.sup.6 to about 10.sup.7 members. This manageable
library size means that libraries of randomized zinc finger
proteins can be efficiently used in high throughput applications to
quickly and reliably identify genes of interest that are associated
with any given phenotype.
[0022] In one aspect, the present invention provides a method of
identifying a gene or genes associated with a selected phenotype,
the method comprising the steps of: (a) providing a nucleic acid
library comprising nucleotide sequences that encode partially
randomized zinc finger proteins; (b) transducing cells with
expression vectors, each comprising a nucleotide sequence from the
library; (c) culturing the cells so that zinc finger proteins are
expressed in the cells, wherein the zinc finger proteins modulate
gene expression in at least some of the cells; (d) assaying the
cells for a selected phenotype and determining whether or not the
cells exhibit the selected phenotype; and (e) identifying, in cells
that exhibit the selected phenotype, the gene or genes whose
expression is modulated by expression of a zinc finger protein,
wherein the gene so identified is associated with the selected
phenotype.
[0023] In one embodiment, the zinc finger protein has three, four,
or five fingers. In another embodiment, the library is made by
finger grafting, DNA shuffling, or codon doping. In another
embodiment, the library comprises no more than about 10.sup.6
clones, no more than about 10.sup.7 clones, or no more than about
10.sup.8 clones.
[0024] In one embodiment, the cells are physically separated,
individual pools of cells and each individual pool of cells is
transduced with an expression vector comprising a nucleotide
sequence from the library. In another embodiment, the physical
separation of the pools of cells is accomplished by placing each
pool of cells in a separate well of a 96, 384, or 1536 well plate.
In another embodiment, the cells are assayed for the selected
phenotype using liquid handling robots. In another embodiment, the
cells are pooled together and transduced in a batch. In another
embodiment, the cells are assayed for the selected phenotype using
flow cytometry. In one embodiment, the cells are selected from the
group consisting of animal cells, plant cells, bacterial cells,
protozoal cells, mammalian cells, human cells, or fungal cells.
[0025] In one embodiment, zinc finger proteins are fusion proteins
comprising one or two regulatory domains, e.g., a transcriptional
repressor, a methyl transferase, a transcriptional activator, a
histone acetyltransferase, and a histone deacetylase. In another
embodiment, the regulatory domain is VP16 or KRAB. In another
embodiment, the zinc finger proteins comprise a Zif268
backbone.
[0026] In one embodiment, modulation of gene expression is
repression of gene expression. In another embodiment, modulation of
gene expression is activation of gene expression. In one
embodiment, expression of the zinc finger proteins is controlled by
administration of a small molecule, e.g., tetracycline.
[0027] In one embodiment, the expression vectors are a viral
vector, e.g., a retroviral expression vector, a lentiviral
expression vector, an adenoviral expression vector, or an AAV
expression vector.
[0028] In one embodiment, the selected phenotype is related to
cancer, nephritis, prostate hypertrophy, hematopoiesis,
osteoporosis, obesity, cardiovascular disease, or diabetes. In one
embodiment, genes that are suspected of being associated with the
selected phenotype are identified by comparing differential gene
expression patterns in the presence and absence of expression of
the zinc finger protein. In another embodiment, differential gene
expression patterns are compared using an oligonucleotide array. In
another embodiment, genes that are suspected of being associated
with the selected phenotype are identified by using zinc finger
proteins from the library of randomized zinc finger proteins to
probe YAC or BAC clones. In another embodiment, genes that are
suspected of being associated with the selected phenotype are
identified by scanning genomic sequences for target sequences
recognized by zinc finger proteins from the library of randomized
zinc finger proteins. In another embodiment, genes that are
suspected of being associated with the selected phenotype are
identified by cross-linking the zinc finger protein to DNA with
which it is associated, followed by immunoprecipitation of the zinc
finger protein and sequencing of the DNA.
BRIEF DESCRIPTION OF THE DRAWINGS
[0029] FIG. 1 shows a zinc finger protein gene assembly using
PCR.
[0030] FIG. 2 shows a diagram of making random zinc finger proteins
with DNA shuffling.
[0031] FIG. 3 shows the life cycle of an adeno-associated
virus.
[0032] FIG. 4 shows high throughput, arrayed generation of AAV-ZFP
vector libraries.
[0033] FIG. 5 shows assaying for a phenotype of interest.
DETAILED DESCRIPTION OF THE INVENTION
[0034] As described herein, the present invention provides
libraries of randomized zinc finger proteins used in screening
assays to identify a gene or genes associated with a selected
phenotype. These libraries of randomized zinc finger proteins can
be readily used to either up- or down-regulate gene expression. No
target DNA sequence information is required to create a random DNA
binding domain. This feature makes the zinc finger protein
technology ideal for screening for genes that are associated with a
desired phenotype. One can simply create a library of randomized
zinc finger-based DNA binding domains, create chimeric up and
down-regulating transcription factors and test the effect of up or
down-regulation on the phenotype under study (transformation,
response to a cytokine etc.) by switching the genes on or off in
any model system.
[0035] Additionally, greater experimental control can be imparted
by zinc finger proteins than can be achieved by more conventional
methods such as antisense, ribozyme, and peptide applications. This
control is available because the expression and/or function of an
engineered zinc finger protein can be placed under small molecule
control. Examples of this approach are provided, e.g., by the
Tet-On system, the ecdysone-regulated system, and the RU-486 system
(see, e.g., Gossen & Bujard, Proc. Natl. Acad. Sci. U.S.A.
89:5547 (1992); Oligino et al., Gene Ther. 5:491-496 (1998); Wang
et al., Gene Ther. 4:432-441 (1997); Neering et al., Blood
88:1147-1155 (1996); and Rendahl et al., Nat. Biotechnol.
16:757-761 (1998)).
[0036] In the present invention, a nucleic acid library of about no
more than 10.sup.6 to 10.sup.7 partially randomized zinc finger
proteins is made, using techniques such as codon doping, gene
shuffling, and finger grafting. Often, a three-fingered zinc
protein is used in the methods of the invention. Cells are then
transfected with the library for expression of a zinc finger
protein clone. Preferably, the zinc finger proteins are introduced
into the cell using viral expression vectors, e.g., retroviral or
adenoviral-based vectors. The cells are then assayed for changes in
the phenotype of choice. Cells can be assayed one by one, using
techniques such as flow cytometry, or in pools of arrayed clonal
populations, using liquid handling robots (see Example section,
below).
[0037] Examples of assay systems for changes in phenotype include,
e.g., transformation assays, e.g., changes in proliferation,
anchorage dependence, growth factor dependence, foci formation, and
growth in soft agar; apoptosis assays, e.g., DNA laddering and cell
death, expression of genes involved in apoptosis; signal
transduction assays, e.g., changes in intracellular calcium, cAMP,
cGMP, IP3, changes in hormone and neurotransmitter release;
receptor assays, e.g., estrogen receptor and cell growth; growth
factor assays, e.g., EPO, hypoxia and erythrocyte colony forming
units assays; enzyme production assays, e.g., FAD-2 induced oil
desaturation; pathogen resistance assays, e.g., insect, bacterial,
and viral resistance assays; chemical production assays, e.g.,
penicillin production; transcription assays, e.g., reporter gene
assays; and protein production assays, e.g., VEGF ELISAs.
[0038] Those cells exhibiting an altered phenotype are selected for
further study, in which the genes associated with the change in
phenotype are identified and isolated. The genes are identified and
isolated, e.g., using differential gene expression analysis with
microarrays; reverse genetics; e.g., identification of genes using
zinc finger proteins to probe YAC or BAC clones and using zinc
finger proteins to scan genomic sequences; subtractive
hybridization; differential cDNA cloning frequencies, subtractive
hybridization; by cloning ESTs from cells of interest; by
identifying genes that are lethal upon knockout; by identifying
genes that are up- or down-regulated in response to a particular
developmental or cellular event or stimuli; by identifying genes
that are up- or down-regulated in certain disease and pathogenic
states; by identifying mutations and RFLPs; by identifying genes
associated with regions of chromosomes known to be involved in
inherited diseases; by identifying genes that are temporally
regulated, e.g., in a pathogenic organism; differences based on
SNPs, etc.
[0039] In one embodiment, the zinc finger protein is linked to at
least one or more regulatory domains, described in detail below.
Preferred regulatory domains include transcription factor repressor
or activator domains such as KRAB and VP16, co-repressor and
co-activator domains, DNA methyltransferases, histone
acetyltransferases, histone deacetylases, and endonucleases such as
Fokl. For repression of gene expression, often simple steric
hindrance of transcription initiation is sufficient.
[0040] Such assays for candidate genes allow for discovery of novel
human and veterinary therapeutic and diagnostic applications,
including the discovery of novel drugs, for, e.g., treatment of
genetic diseases, cancer, fungal, protozoal, bacterial, and viral
infection, ischemia, vascular disease, arthritis, immunological
disorders, etc. In addition, the methods of the invention can be
used in the agricultural industry for the identification of
commercially relevant plant genes, and can be used to engineer
bacteria and other organisms to produce industrial chemicals and
pharmaceuticals.
Definitions
[0041] As used herein, the following terms have the meanings
ascribed to them unless specified otherwise.
[0042] "Partially randomized" zinc finger proteins refers to a zinc
finger protein where at least some of the amino acids of any
individual finger are generated randomly and are not preselected
(e.g., the four critical amino acids of finger 1), or wherein at
least one finger or part of a finger from a known zinc finger
protein is randomly combined with another heterologous finger or
part of a finger from a known zinc finger protein. Typically, a
standard zinc finger protein backbone from a mammalian zinc finger
protein such as SP1 or Zif268 is used to make the partially random
protein, with the fingers either partially or fully randomized via
random codon selection. In some cases the codons are partially
randomized, e.g., to eliminate termination codons (see Table 2,
below). Partially random zinc finger proteins include fully
randomized zinc finger proteins. In one embodiment, amino acids -1,
2, 3, and 6 of a finger are randomly selected.
[0043] A "gene associated with a selected phenotype" refers to a
cellular, viral, bacterial, protozoal, fungal, animal, plant,
episomal, chloroplastic, or mitochondrial gene, where modulation of
gene expression using a randomized zinc finger protein causes a
change in the selected phenotype. This term also refers to a
microbial or viral gene that is part of a naturally occurring
microbial or viral genome in a microbially or virally infected
cell. The microbial or viral genome can be extrachromosomal or
integrated into the host chromosome. This term also encompasses
endogenous and exogenous genes, as well as cellular genes that are
identified as expressed sequence tags ("ESTs"). An assay of choice
is used to identify genes associated with a selected phenotype upon
regulation of gene expression with a zinc finger protein. The genes
are typically identified via methods such as gene expression
microarrays, differential cDNA cloning frequencies, subtractive
hybridization and differential display methods. The genes
associated with a selected phenotype are then subjected to target
validation using engineered zinc finger proteins (see, e.g.,
copending patent application U.S. Ser. No. 09/395,448, filed Sep.
14, 1999).
[0044] A "selected phenotype" refers to any phenotype, e.g., any
observable characteristic such as a physical, chemical, or
functional effect that can be measured in an assay such as changes
in cell growth, proliferation, morphology, enzyme function, signal
transduction, expression patterns, downstream expression patterns,
reporter gene activation, hormone release, growth factor release,
neurotransmitter release, ligand binding, apoptosis, and product
formation. Such assays include, e.g., transformation assays, e.g.,
changes in proliferation, anchorage dependence, growth factor
dependence, foci formation, and growth in soft agar; apoptosis
assays, e.g., DNA laddering and cell death, expression of genes
involved in apoptosis; signal transduction assays, e.g., changes in
intracellular calcium, cAMP, cGMP, IP3, changes in hormone and
neurotransmitter release; receptor assays, e.g., estrogen receptor
and cell growth; growth factor assays, e.g., EPO, hypoxia and
erythrocyte colony forming units assays; enzyme production assays,
e.g., FAD-2 induced oil desaturation; pathogen resistance assays,
e.g., insect, bacterial, and viral resistance assays; chemical
production assays, e.g., penicillin production; transcription
assays, e.g., reporter gene assays; and protein production assays,
e.g., VEGF ELISAs.
[0045] The term "zinc finger protein" or "ZFP" refers to a protein
having DNA binding domains that are stabilized by zinc. The
individual DNA binding domains are typically referred to as
"fingers" A zinc finger protein has at least one finger, typically
two fingers, three fingers, four fingers, five fingers, or six
fingers or more. Each finger binds from two to four base pairs of
DNA, typically three or four base pairs of DNA. A zinc finger
protein binds to a nucleic acid sequence called a target site or
target segment. Each finger typically comprises an approximately 30
amino acid, zinc-coordinating, DNA-binding subdomain. An exemplary
motif characterizing one class of these proteins
(Cys.sub.2His.sub.2 class) is
-Cys-(X).sub.2-4-Cys-(X).sub.12-His-(X).sub.3-5-His (where X is any
amino acid). Studies have demonstrated that a single zinc finger of
this class consists of an alpha helix containing the two invariant
histidine residues co-ordinated with zinc along with the two
cysteine residues of a single beta turn (see, e.g., Berg & Shi,
Science 271:1081-1085 (1996)).
[0046] A "target site" is the nucleic acid sequence recognized by a
zinc finger protein. A single target site typically has about four
to about ten or more base pairs. Typically, a two-fingered zinc
finger protein recognizes a four to seven base pair target site, a
three-fingered zinc finger protein recognizes a six to ten base
pair target site, a six fingered zinc finger protein recognizes two
adjacent nine to ten base pair target sites, and so on for proteins
with more than six fingers. The target site is in any position that
allows regulation of gene expression, e.g., adjacent to, up- or
downstream of the transcription initiation site; proximal to an
enhancer or other transcriptional regulation element such as a
repressor (e.g., SP-1 binding sites, hypoxia response elements,
nuclear receptor recognition elements, p53 binding sites, etc.),
RNA polymerase pause sites; and intron/exon boundaries. The term
"adjacent target sites" refers to non-overlapping target sites that
are separated by zero to about 5 base pairs.
[0047] "K.sub.d" refers to the dissociation constant for the
compound, i.e., the concentration of a compound (e.g., a zinc
finger protein) that gives half maximal binding of the compound to
its target (i.e., half of the compound molecules are bound to the
target) under given conditions (i.e., when
[target]<<K.sub.d), as measured using a given assay system
(see, e.g., U.S. Pat. No. 5,789,538). The assay system used to
measure the K.sub.d should be chosen so that it gives the most
accurate measure of the actual K.sub.d of the zinc finger protein.
Any assay system can be used, as long is it gives an accurate
measurement of the actual K.sub.d of the zinc finger protein. In
one embodiment, the K.sub.d for the zinc finger proteins of the
invention is measured using an electrophoretic mobility shift assay
("EMSA"), as described herein. Unless an adjustment is made for
zinc finger protein purity or activity, the K.sub.d calculations
made using the methods described herein may result in an
underestimate of the true K.sub.d of a given zinc finger protein.
Optionally, the K.sub.d of a zinc finger protein used to modulate
transcription of a candidate gene is less than about 100 nM, or
less than about 75 nM, or less than about 50 nM, or less than about
25 nM.
[0048] "Administering" an expression vector, nucleic acid, zinc
finger protein, or a delivery vehicle to a cell comprises
transducing, transfecting, electroporating, translocating, fusing,
phagocytosing, or ballistic methods, etc., i.e., any means by which
a protein or nucleic acid can be transported across a cell membrane
and preferably into the nucleus of a cell, including administration
of naked DNA.
[0049] A "delivery vehicle" refers to a compound, e.g., a liposome,
toxin, or a membrane translocation polypeptide, which is used to
administer a zinc finger protein. Delivery vehicles can also be
used to administer nucleic acids encoding zinc finger proteins,
e.g., a lipid:nucleic acid complex, an expression vector, a virus,
and the like.
[0050] The terms "modulating expression" "inhibiting expression"
and "activating expression" of a gene refer to the ability of a
zinc finger protein to activate or inhibit transcription of a gene.
Activation includes prevention of transcriptional inhibition (i.e.,
prevention of repression of gene expression) and inhibition
includes prevention of transcriptional activation (i.e., prevention
of gene activation).
[0051] "Activation of gene expression that prevents repression of
gene expression" refers to the ability of a zinc finger protein to
block the action of or prevent binding of a repressor molecule.
[0052] "Inhibition of gene expression that prevents gene
activation" refers to the ability of a zinc finger protein to block
the action of or prevent binding of an activator molecule.
[0053] Modulation can be assayed by determining any parameter that
is indirectly or directly affected by the expression of the target
gene. Such parameters include, e.g., changes in RNA or protein
levels, changes in protein activity, changes in product levels,
changes in downstream gene expression, changes in reporter gene
transcription (luciferase, CAT, .beta.-galactosidase,
.beta.-glucuronidase, GFP (see, e.g., Mistili & Spector, Nature
Biotechnology 15:961-964 (1997)); changes in signal transduction,
phosphorylation and dephosphorylation, receptor-ligand
interactions, second messenger concentrations (e.g., cGMP, cAMP,
IP3, and Ca.sup.2+), and cell growth, etc., as described herein.
These assays can be in vitro, in vivo, and ex vivo. Such functional
effects can be measured by any means known to those skilled in the
art, e.g., measurement of RNA or protein levels, measurement of RNA
stability, identification of downstream or reporter gene
expression, e.g., via chemiluminescence, fluorescence, fluorescent
activated cell sorting ("FACS"), calorimetric reactions, antibody
binding, inducible markers, ligand binding assays; changes in
intracellular second messengers such as cGMP and inositol
triphosphate (IP3); changes in intracellular calcium levels;
cytokine release, and the like, as described herein.
[0054] To determine the level of gene expression modulation
effected by a zinc finger protein, cells contacted with zinc finger
proteins are compared to control cells, e.g., without the zinc
finger protein or with a non-specific zinc finger protein, to
examine the extent of inhibition or activation. Control samples are
assigned a relative gene expression activity value of 100%.
Modulation/inhibition of gene expression is achieved when the gene
expression activity value relative to the control is about 80%,
preferably 50% (i.e., 0.5.times. the activity of the control), more
preferably 25%, more preferably 5-0%. Modulation/activation of gene
expression is achieved when the gene expression activity value
relative to the control is 110%, more preferably 150% (i.e.,
1.5.times. the activity of the control), more preferably 200-500%,
more preferably 1000-2000% or more.
[0055] A "transcriptional activator" and a "transcriptional
repressor" refer to proteins or effector domains of proteins that
have the ability to modulate transcription, as described above.
Such proteins include, e.g., transcription factors and co-factors
(e.g., KRAB, MAD, ERD, SID, nuclear factor kappa B subunit p65,
early growth response factor 1, and nuclear hormone receptors, VP
16, VP64), endonucleases, integrases, recombinases,
methyltransferases, histone acetyltransferases, histone
deacetylases etc. Activators and repressors include co-activators
and co-repressors (see, e.g., Utley et al., Nature 394:498-502
(1998)).
[0056] A "regulatory domain" refers to a protein or a protein
domain that has transcriptional modulation activity when tethered
to a DNA binding domain, i.e., a zinc finger protein. Typically, a
regulatory domain is covalently or non-covalently linked to a zinc
finger protein to effect transcription modulation. Alternatively, a
zinc finger protein can act alone, without a regulatory domain, to
effect transcription modulation.
[0057] The term "heterologous" is a relative term, which when used
with reference to portions of a nucleic acid indicates that the
nucleic acid comprises two or more subsequences that are not found
in the same relationship to each other in nature. For instance, a
nucleic acid that is recombinantly produced typically has two or
more sequences from unrelated genes synthetically arranged to make
a new functional nucleic acid, e.g., a promoter from one source and
a coding region from another source. The two nucleic acids are thus
heterologous to each other in this context. When added to a cell,
the recombinant nucleic acids would also be heterologous to the
endogenous genes of the cell. Thus, in a chromosome, a heterologous
nucleic acid would include an non-native (non-naturally occurring)
nucleic acid that has integrated into the chromosome, or a
non-native (non-naturally occurring) extrachromosomal nucleic
acid.
[0058] Similarly, a heterologous protein indicates that the protein
comprises two or more subsequences that are not found in the same
relationship to each other in nature (e.g., a "fusion protein,"
where the two subsequences are encoded by a single nucleic acid
sequence). See, e.g., Ausubel, supra, for an introduction to
recombinant techniques.
[0059] The term "recombinant" when used with reference, e.g., to a
cell, or nucleic acid, protein, or vector, indicates that the cell,
nucleic acid, protein or vector, has been modified by the
introduction of a heterologous nucleic acid or protein or the
alteration of a native nucleic acid or protein, or that the cell is
derived from a cell so modified. Thus, for example, recombinant
cells express genes that are not found within the native (naturally
occurring) form of the cell or express a second copy of a native
gene that is otherwise normally or abnormally expressed, under
expressed or not expressed at all.
[0060] A "promoter" is defined as an array of nucleic acid control
sequences that direct transcription. As used herein, a promoter
typically includes necessary nucleic acid sequences near the start
site of transcription, such as, in the case of certain RNA
polymerase II type promoters, a TATA element, enhancer, CCAAT box,
SP-1 site, etc. As used herein, a promoter also optionally includes
distal enhancer or repressor elements, which can be located as much
as several thousand base pairs from the start site of
transcription. The promoters often have an element that is
responsive to transactivation by a DNA-binding moiety such as a
polypeptide, e.g., a nuclear receptor, Gal4, the lac repressor and
the like.
[0061] A "constitutive" promoter is a promoter that is active under
most environmental and developmental conditions. An "inducible"
promoter is a promoter that is active under certain environmental
or developmental conditions.
[0062] The term "operably linked" refers to a functional linkage
between a nucleic acid expression control sequence (such as a
promoter, or array of transcription factor binding sites) and a
second nucleic acid sequence, wherein the expression control
sequence directs transcription of the nucleic acid corresponding to
the second sequence.
[0063] An "expression vector" is a nucleic acid construct,
generated recombinantly or synthetically, with a series of
specified nucleic acid elements that permit transcription of a
particular nucleic acid in a host cell, and optionally, integration
or replication of the expression vector in a host cell. The
expression vector can be part of a plasmid, virus, or nucleic acid
fragment, of viral or non-viral origin. Typically, the expression
vector includes an "expression cassette," which comprises a nucleic
acid to be transcribed operably linked to a promoter. The term
expression vector also encompasses naked DNA operably linked to a
promoter.
[0064] By "host cell" is meant a cell that contains a zinc finger
protein or an expression vector or nucleic acid encoding a zinc
finger protein. The host cell typically supports the replication
and/or expression of the expression vector. Host cells may be
prokaryotic cells such as E. coli, or eukaryotic cells such as
yeast, fungal, protozoal, higher plant, insect, or amphibian cells,
or mammalian cells such as CHO, HeLa, 293, COS-1, and the like,
e.g., cultured cells (in vitro), explants and primary cultures (in
vitro and ex vivo), and cells in vivo.
[0065] "Nucleic acid" refers to deoxyribonucleotides or
ribonucleotides and polymers thereof in either single- or
double-stranded form. The term encompasses nucleic acids containing
known nucleotide analogs or modified backbone residues or linkages,
which are synthetic, naturally occurring, and non-naturally
occurring, which have similar binding properties as the reference
nucleic acid, and which are metabolized in a manner similar to the
reference nucleotides. Examples of such analogs include, without
limitation, phosphorothioates, phosphoramidates, methyl
phosphonates, chiral-methyl phosphonates, 2-O-methyl
ribonucleotides, peptide-nucleic acids (PNAs).
[0066] Unless otherwise indicated, a particular nucleic acid
sequence also implicitly encompasses conservatively modified
variants thereof (e.g., degenerate codon substitutions) and
complementary sequences, as well as the sequence explicitly
indicated. Specifically, degenerate codon substitutions may be
achieved by generating sequences in which the third position of one
or more selected (or all) codons is substituted with mixed-base
and/or deoxyinosine residues (Batzer et al., Nucleic Acid Res.
19:5081 (1991); Ohtsuka et al., J. Biol. Chem. 260:2605-2608
(1985); Rossolini et al., Mol. Cell. Probes 8:91-98 (1994)). The
term nucleic acid is used interchangeably with gene, cDNA, mRNA,
oligonucleotide, and polynucleotide.
[0067] The terms "polypeptide," "peptide" and "protein" are used
interchangeably herein to refer to a polymer of amino acid
residues. The terms also apply to amino acid polymers in which one
or more amino acid residues is an artificial chemical mimetic of a
corresponding naturally occurring amino acid, as well as to
naturally occurring amino acid polymers and non-naturally occurring
amino acid polymer.
[0068] The term "amino acid" refers to naturally occurring and
synthetic amino acids, as well as amino acid analogs and amino acid
mimetics that function in a manner similar to the naturally
occurring amino acids. Naturally occurring amino acids are those
encoded by the genetic code, as well as those amino acids that are
later modified, e.g., hydroxyproline, y-carboxyglutamate, and
O-phosphoserine. Amino acid analogs refers to compounds that have
the same basic chemical structure as a naturally occurring amino
acid, i.e., an a carbon that is bound to a hydrogen, a carboxyl
group, an amino group, and an R group, e.g., homoserine,
norleucine, methionine sulfoxide, methionine methyl sulfonium. Such
analogs have modified R groups (e.g., norleucine) or modified
peptide backbones, but retain the same basic chemical structure as
a naturally occurring amino acid. Amino acid mimetics refers to
chemical compounds that have a structure that is different from the
general chemical structure of an amino acid, but that functions in
a manner similar to a naturally occurring amino acid.
[0069] Amino acids may be referred to herein by either their
commonly known three letter symbols or by the one-letter symbols
recommended by the IUPAC-IUB Biochemical Nomenclature Commission.
Nucleotides, likewise, may be referred to by their commonly
accepted single-letter codes.
[0070] "Conservatively modified variants" applies to both amino
acid and nucleic acid sequences. With respect to particular nucleic
acid sequences, conservatively modified variants refers to those
nucleic acids which encode identical or essentially identical amino
acid sequences, or where the nucleic acid does not encode an amino
acid sequence, to essentially identical sequences. Because of the
degeneracy of the genetic code, a large number of functionally
identical nucleic acids encode any given protein. For instance, the
codons GCA, GCC, GCG and GCU all encode the amino acid alanine.
Thus, at every position where an alanine is specified by a codon,
the codon can be altered to any of the corresponding codons
described without altering the encoded polypeptide. Such nucleic
acid variations are "silent variations," which are one species of
conservatively modified variations. Every nucleic acid sequence
herein which encodes a polypeptide also describes every possible
silent variation of the nucleic acid. One of skill will recognize
that each codon in a nucleic acid (except AUG, which is ordinarily
the only codon for methionine, and TGG, which is ordinarily the
only codon for tryptophan) can be modified to yield a functionally
identical molecule. Accordingly, each silent variation of a nucleic
acid which encodes a polypeptide is implicit in each described
sequence.
[0071] As to amino acid sequences, one of skill will recognize that
individual substitutions, deletions or additions to a nucleic acid,
peptide, polypeptide, or protein sequence which alters, adds or
deletes a single amino acid or a small percentage of amino acids in
the encoded sequence is a "conservatively modified variant" where
the alteration results in the substitution of an amino acid with a
chemically similar amino acid. Conservative substitution tables
providing functionally similar amino acids are well known in the
art. Such conservatively modified variants are in addition to and
do not exclude polymorphic variants, interspecies homologs, and
alleles of the invention.
[0072] The following eight groups each contain amino acids that are
conservative substitutions for one another:
1) Alanine (A), Glycine (G);
2) Aspartic acid (D), Glutamic acid (E);
3) Asparagine (N), Glutamine (Q);
4) Arginine (R), Lysine (K);
5) Isoleucine (I), Leucine (L), Methionine (M), Valine (V);
6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W);
7) Serine (S), Threonine (T); and
8) Cysteine (C), Methionine (M)
(see, e.g., Creighton, Proteins (1984)).
Making Libraries of Randomized Zinc Finger Proteins
[0073] Libraries of nucleic acids encoding randomized zinc finger
proteins are generated for use in the methods of the invention.
Typically, a backbone from any suitable Cys.sub.2His.sub.2 zinc
finger protein, such as SP-1, SP-1C, or ZIF268, is used as the
scaffold for the randomized zinc finger protein (see, e.g., Jacobs,
EMBO J. 11:4507 (1992); Desjarlais & Berg, Proc. Nat'l Acad.
Sci. USA 90:2256-2260 (1993)). A number of methods can then be used
to generate libraries of nucleic acids encoding the randomized zinc
finger proteins.
[0074] At least three different strategies can be used to make the
random zinc finger protein libraries. The first method, called the
finger or recognition helix grafting strategy, will typically have
the least non-functional zinc finger proteins, and the
recombination is limited only to the existing fingers. The second
method, called the codon doping strategy, provides the most
complete randomization scheme. The third method, called the gene
shuffling strategy, will generate new variants for all fingers. In
this method, however, the mutagenesis is not complete but is
derived from only a limited number of parental zinc finger
proteins. The three randomization schemes can be used herein to
build the randomized zinc finger protein libraries and to test the
libraries for DNA binding in vitro by using a phage display system
(see Example section, below).
[0075] In one embodiment, the method used for zinc finger protein
library construction is fingertip, or recognition helix grafting.
Imagine a collection of 3-bp binding zinc finger protein helices
that could be grafted together in any combination. Each unique
multi-finger combination would recognize a different unique DNA
sequence. The number of different fingers used and the number of
fingers attached together can be varied in this method. In one
embodiment, the number of different fingertips is about 10-14,
optionally 12, and the number of fingers is 3-5, optionally 5, and
a randomized zinc finger library size to screen preferably consists
of 250,000+ members.
[0076] There are about 140,000 genes in the human genome and the
human genome has about 3.times.10.sup.9 basepairs. In one
embodiment, a library of five finger zinc finger proteins made with
20 different fingertips would recognize about 3,200,000 different
15 basepair sequences (205); a library made with 15 different
fingertips would recognize about 759,375 different sequences; a
library made with 13 fingertips would recognize about 371,293
different sequences; and a library made with 12 fingertips would
recognize about 248,832. Using specific helices for all 64 triplets
would be sufficient to recognize any and all 15 basepair
sequences.
[0077] Considering both strands of the target genome, a 15 basepair
sequence is expected to occur 0.6 times
((2.8.times.10.sup.8/4.sup.15).times.2). In other words, of a
random 5-finger library, at least 60 percent of the component zinc
finger proteins are expected to affect the expression of a single
gene. Considering the entire genome, a random 5-finger zinc finger
protein is expected to have on average only 6 perfect binding
sites.
[0078] On average, no more than one gene should be directly
affected at a time by a component zinc finger protein, and only a
handful of genomic binding sites need to be considered. In fact,
the active zinc finger protein itself can be used to identify
candidate genes either by sequence scanning or as probes to
identify candidate genomic clones (i.e., from YAC or BAC
clones).
[0079] In addition, any other suitable method known in the art can
be used to construct nucleic acids encoding random zinc finger
proteins, e.g., phage display, random mutagenesis, combinatorial
libraries, affinity selection, PCR, cloning from cDNA or genomic
libraries, synthetic construction and the like. (see, e.g., U.S.
Pat. No. 5,786,538; Wu et al., Proc. Nat'l Acad. Sci. USA
92:344-348 (1995); Jamieson et al., Biochemistry 33:5689-5695
(1994); Rebar & Pabo, Science 263:671-673 (1994); Choo &
Klug, Proc. Nat'l Acad. Sci. USA 91:11163-11167 (1994); Choo &
Klug, Proc. Nat'lAcad. Sci. USA 91:11168-11172(1994); Desjarlais
& Berg, Proc. Nat'l Acad. Sci. USA 90:2256-2260 (1993);
Desjarlais & Berg, Proc. Nat'l Acad. Sci. USA 89:7345-7349
(1992); Pomerantz et al., Science 267:93-96 (1995); Pomerantz et
al., Proc. Nat'l Acad. Sci. USA 92:9752-9756 (1995); and Liu et
al., Proc. Nat'l Acad. Sci. USA 94:5525-5530 (1997); Greisman &
Pabo, Science 275:657-661 (1997); Desjarlais & Berg, Proc.
Nat'l Acad. Sci. USA 91:11-99-11103 (1994)).
Regulatory Domains
[0080] The zinc finger proteins of the invention can optionally be
associated with regulatory domains for modulation of gene
expression. The zinc finger protein can be covalently or
non-covalently associated with one or more regulatory domains,
alternatively two or more regulatory domains, with the two or more
domains being two copies of the same domain, or two different
domains. The regulatory domains can be covalently linked to the
zinc finger protein, e.g., via an amino acid linker, as part of a
fusion protein. The zinc finger proteins can also be associated
with a regulatory domain via a non-covalent dimerization domain,
e.g., a leucine zipper, a STAT protein N terminal domain, or an
FK506 binding protein (see, e.g., O'Shea, Science 254: 539 (1991),
Barahmand-Pour et al., Curr. Top. Microbiol. Immunol. 211:121-128
(1996); Klemm et al., Annu. Rev. Immunol. 16:569-592 (1998); Klemm
et al., Annu. Rev. Immunol. 16:569-592 (1998); Ho et al., Nature
382:822-826 (1996); and Pomeranz et al., Biochem. 37:965 (1998)).
The regulatory domain can be associated with the zinc finger
protein at any suitable position, including the C- or N-terminus of
the zinc finger protein.
[0081] Common regulatory domains for addition to the zinc finger
protein include, e.g., effector domains from transcription factors
(activators, repressors, co-activators, co-repressors), silencers,
nuclear hormone receptors, oncogene transcription factors (e.g.,
myc, jun, fos, myb, max, mad, rel, ets, bcl, mos family members
etc.); DNA repair enzymes and their associated factors and
modifiers; DNA rearrangement enzymes and their associated factors
and modifiers; chromatin associated proteins and their modifiers
(e.g., kinases, acetylases and deacetylases); and DNA modifying
enzymes (e.g., methyltransferases, topoisomerases, helicases,
ligases, kinases, phosphatases, polymerases, endonucleases) and
their associated factors and modifiers.
[0082] Transcription factor polypeptides from which one can obtain
a regulatory domain include those that are involved in regulated
and basal transcription. Such polypeptides include transcription
factors, their effector domains, coactivators, silencers, nuclear
hormone receptors (see, e.g., Goodrich et al., Cell 84:825-30
(1996) for a review of proteins and nucleic acid elements involved
in transcription; transcription factors in general are reviewed in
Barnes & Adcock, Clin. Exp. Allergy 25 Suppl. 2:46-9 (1995) and
Roeder, Methods Enzymol. 273:165-71 (1996)). Databases dedicated to
transcription factors are known (see, e.g., Science 269:630
(1995)). Nuclear hormone receptor transcription factors are
described in, for example, Rosen et al., J. Med. Chem. 38:4855-74
(1995). The C/EBP family of transcription factors are reviewed in
Wedel et al., Immunobiology 193:171-85 (1995). Coactivators and
co-repressors that mediate transcription regulation by nuclear
hormone receptors are reviewed in, for example, Meier, Eur. J.
Endocrinol. 134(2):158-9 (1996); Kaiser et al., Trends Biochem.
Sci. 21:342-5 (1996); and Utley et al., Nature 394:498-502 (1998)).
GATA transcription factors, which are involved in regulation of
hematopoiesis, are described in, for example, Simon, Nat. Genet.
11:9-11 (1995); Weiss et al., Exp. Hematol. 23:99-107. TATA box
binding protein (TBP) and its associated TAF polypeptides (which
include TAF30, TAF55, TAF80, TAF110, TAF150, and TAF250) are
described in Goodrich & Tjian, Curr. Opin. Cell Biol. 6:403-9
(1994) and Hurley, Curr. Opin. Struct. Biol. 6:69-75 (1996). The
STAT family of transcription factors are reviewed in, for example,
Barahmand-Pour et al., Curr. Top. Microbiol. Immunol. 211:121-8
(1996). Transcription factors involved in disease are reviewed in
Aso et al., J. Clin. Invest. 97:1561-9 (1996).
[0083] In one embodiment, the KRAB repression domain from the human
KOX-1 protein is used as a transcriptional repressor (Thiesen et
al., New Biologist 2:363-374 (1990); Margolin et al., Proc. Nat'l
Acad. Sci. USA 91:4509-4513 (1994); Pengue et al., Nucl. Acids Res.
22:2908-2914 (1994); Witzgall et al., Proc. Nat'l Acad. Sci. USA
91:4514-4518 (1994)). In another embodiment, KAP-1, a KRAB
co-repressor, is used with KRAB (Friedman et al., Genes Dev.
10:2067-2078 (1996)). Alternatively, KAP-1 can be used alone with a
zinc finger protein. Other preferred transcription factors and
transcription factor domains that act as transcriptional repressors
include MAD (see, e.g., Sommer et al., J. Biol. Chem. 273:6632-6642
(1998); Gupta et al., Oncogene 16:1149-1159 (1998); Queva et al.,
Oncogene 16:967-977 (1998); Larsson et al., Oncogene 15:737-748
(1997); Laherty et al., Cell 89:349-356 (1997); and Cultraro et
al., Mol Cell. Biol. 17:2353-2359 (19977)); FKHR (forkhead in
rhapdosarcoma gene; Ginsberg et al., Cancer Res. 15:3542-3546
(1998); Epstein et al., Mol. Cell. Biol. 18:4118-4130 (1998));
EGR-1 (early growth response gene product-1; Yan et al., Proc.
Nat'l Acad. Sci. USA 95:8298-8303 (1998); and Liu et al., Cancer
Gene Ther. 5:3-28 (1998)); the ets2 repressor factor repressor
domain (ERD; Sgouras et al., EMBO J. 14:4781-4793 (1995)); and the
MAD smSIN3 interaction domain (SID; Ayer et al., Mol. Cell. Biol.
16:5772-5781 (1996)).
[0084] In one embodiment, the HSV VP16 activation domain is used as
a transcriptional activator (see, e.g., Hagmann et al., J. Virol.
71:5952-5962 (1997)). Other preferred transcription factors that
could supply activation domains include the VP64 activation domain
(Seipel et al., EMBO J. 11:4961-4968 (1996)); nuclear hormone
receptors (see, e.g., Torchia et al., Curr. Opin. Cell. Biol.
10:373-383 (1998)); the p65 subunit of nuclear factor kappa B
(Bitko & Barik, J. Virol. 72:5610-5618 (1998) and Doyle &
Hunt, Neuroreport 8:2937-2942 (1997)); and EGR-1 (early growth
response gene product-1; Yan et al., Proc. Nat'l Acad. Sci. USA
95:8298-8303 (1998); and Liu et al., Cancer Gene Ther. 5:3-28
(1998)).
[0085] Kinases, phosphatases, and other proteins that modify
polypeptides involved in gene regulation are also useful as
regulatory domains for zinc finger proteins. Such modifiers are
often involved in switching on or off transcription mediated by,
for example, hormones. Kinases involved in transcription regulation
are reviewed in Davis, Mol. Reprod. Dev. 42:459-67 (1995), Jackson
et al., Adv. Second Messenger Phosphoprotein Res. 28:279-86 (1993),
and Boulikas, Crit. Rev. Eukaryot. Gene Expr. 5:1-77 (1995), while
phosphatases are reviewed in, for example, Schonthal & Semin,
Cancer Biol. 6:239-48 (1995). Nuclear tyrosine kinases are
described in Wang, Trends Biochem. Sci. 19:373-6 (1994).
[0086] As described, useful domains can also be obtained from the
gene products of oncogenes (e.g., myc, jun, fos, myb, max, mad,
rel, ets, bcl, mos family members) and their associated factors and
modifiers. Oncogenes are described in, for example, Cooper,
Oncogenes, The Jones and Bartlett Series in Biology (2.sup.nd ed.,
1995). The ets transcription factors are reviewed in Waslylk et
al., Eur. J. Biochem. 211:7-18 (1993) and Crepieux et al., Crit.
Rev. Oncog. 5:615-38 (1994). Myc oncogenes are reviewed in, for
example, Ryan et al., Biochem. J. 314:713-21 (1996). The jun and
fos transcription factors are described in, for example, The Fos
and Jun Families of Transcription Factors (Angel & Herrlich,
eds. 1994). The max oncogene is reviewed in Hurlin et al., Cold
Spring Harb. Symp. Quant. Biol. 59:109-16. The myb gene family is
reviewed in Kanei-Ishii et al., Curr. Top. Microbiol. Immunol.
211:89-98 (1996). The mos family is reviewed in Yew et al., Curr.
Opin. Genet. Dev. 3:19-25 (1993).
[0087] Zinc finger proteins can include regulatory domains obtained
from DNA repair enzymes and their associated factors and modifiers.
DNA repair systems are reviewed in, for example, Vos, Curr. Opin.
Cell Biol. 4:385-95 (1992); Sancar, Ann. Rev. Genet. 29:69-105
(1995); Lehmann, Genet. Eng. 17:1-19 (1995); and Wood, Ann. Rev.
Biochem. 65:135-67 (1996). DNA rearrangement enzymes and their
associated factors and modifiers can also be used as regulatory
domains (see, e.g., Gangloff et al., Experientia 50:261-9 (1994);
Sadowski, FASEB J. 7:760-7 (1993)).
[0088] Similarly, regulatory domains can be derived from DNA
modifying enzymes (e.g., DNA methyltransferases, topoisomerases,
helicases, ligases, kinases, phosphatases, polymerases) and their
associated factors and modifiers. Helicases are reviewed in Matson
et al., Bioessays, 16:13-22 (1994), and methyltransferases are
described in Cheng, Curr. Opin. Struct. Biol. 5:4-10 (1995).
Chromatin associated proteins and their modifiers (e.g., kinases,
acetylases and deacetylases), such as histone deacetylase (Wolffe,
Science 272:371-2 (1996)) are also useful as domains for addition
to the zinc finger protein of choice. In one preferred embodiment,
the regulatory domain is a DNA methyl transferase that acts as a
transcriptional repressor (see, e.g., Van den Wyngaert et al., FEBS
Lett. 426:283-289 (1998); Flynn et al., J. Mol. Biol. 279:101-116
(1998); Okano et al., Nucleic Acids Res. 26:2536-2540 (1998); and
Zardo & Caiafa, J. Biol. Chem. 273:16517-16520 (1998)). In
another preferred embodiment, endonucleases such as Fokl are used
as transcriptional repressors, which act via gene cleavage (see,
e.g., WO95/09233; and PCT/US94/01201).
[0089] Factors that control chromatin and DNA structure, movement
and localization and their associated factors and modifiers;
factors derived from microbes (e.g., prokaryotes, eukaryotes and
virus) and factors that associate with or modify them can also be
used to obtain chimeric proteins. In one embodiment, recombinases
and integrases are used as regulatory domains. In one embodiment,
histone acetyltransferase is used as a transcriptional activator
(see, e.g., Jin & Scotto, Mol. Cell. Biol. 18:4377-4384 (1998);
Wolffe, Science 272:371-372 (1996); Taunton et al., Science
272:408-411 (1996); and Hassig et al., Proc. Nat'l Acad. Sci. USA
95:3519-3524 (1998)). In another embodiment, histone deacetylase is
used as a transcriptional repressor (see, e.g., Jin & Scotto,
Mol. Cell. Biol. 18:4377-4384 (1998); Syntichaki & Thireos, J.
Biol. Chem. 273:24414-24419 (1998); Sakaguchi et al., Genes Dev.
12:2831-2841 (1998); and Martinez et al., J. Biol. Chem.
273:23781-23785 (1998)).
[0090] Linker domains between polypeptide domains, e.g., between
two zinc finger proteins or between a zinc finger protein and a
regulatory domain, can be included. Such linkers are typically
polypeptide sequences, such as poly gly sequences of between about
5 and 200 amino acids. Preferred linkers are typically flexible
amino acid subsequences which are synthesized as part of a
recombinant fusion protein. For example, in one embodiment, the
linker DGGGS is used to link two zinc finger proteins. In another
embodiment, the flexible linker linking two zinc finger proteins is
an amino acid subsequence comprising the sequence TGEKP (see, e.g.,
Liu et al., Proc. Nat'l Acad. Sci. USA 5525-5530 (1997)). In
another embodiment, the linker LRQKDGERP is used to link two zinc
finger proteins. In another embodiment, the following linkers are
used to link two zinc finger proteins: GGRR (Pomerantz et al. 1995,
supra), (G4S).sub.n (Kim et al., Proc. Nat'l Acad. Sci. USA 93,
1156-1160 (1996.); and GGRRGGGS; LRQRDGERP; LRQKDGGGSERP;
LRQKD(G3S).sub.2 ERP. Alternatively, flexible linkers can be
rationally designed using computer program capable of modeling both
DNA-binding sites and the peptides themselves (Desjarlais &
Berg, Proc. Nat'l Acad. Sci. USA 90:2256-2260 (1993), Proc. Nat'l
Acad. Sci. USA 91:11099-11103 (1994) or by phage display
methods.
[0091] In other embodiments, a chemical linker is used to connect
synthetically or recombinantly produced domain sequences. Such
flexible linkers are known to persons of skill in the art. For
example, poly(ethylene glycol) linkers are available from
Shearwater Polymers, Inc. Huntsville, Ala. These linkers optionally
have amide linkages, sulfhydryl linkages, or heterofunctional
linkages. In addition to covalent linkage of zinc finger proteins
to regulatory domains, non-covalent methods can be used to produce
molecules with zinc finger proteins associated with regulatory
domains.
[0092] In addition to regulatory domains, often the zinc finger
protein is expressed as a fusion protein such as maltose binding
protein ("MBP"), glutathione S transferase ("GST"), hexahistidine,
c-myc, and the FLAG epitope, for ease of purification, monitoring
expression, or monitoring cellular and subcellular
localization.
Expression Vectors and Introduction of Random Libraries Into
Cells
[0093] A. Cloning and Expression of Libraries Encoding Randomized
Zinc Finger Proteins
[0094] Nucleic acids encoding the randomized zinc finger proteins
are typically cloned into vectors for transformation into
prokaryotic or eukaryotic cells for replication, expression, and
cell transformation. Such vectors are typically prokaryotic
vectors, e.g., plasmids that act as shuttle vectors; eukaryotic
vectors such as insect vectors, for storage, manipulation of the
nucleic acid encoding zinc finger protein or production of protein;
or eukaryotic vectors such as viral vectors (e.g., adenoviral
vectors, retroviral vector, etc.) for expression of zinc finger
proteins and regulation of gene expression. The nucleic acid
encoding a zinc finger protein can then be administered to a plant
cell, animal cell, a mammalian cell or a human cell, a fungal cell,
a bacterial cell, or a protozoal cell.
[0095] To obtain expression of a cloned gene or nucleic acid, a
zinc finger protein is typically subcloned into an expression
vector that contains a promoter to direct transcription. Suitable
bacterial and eukaryotic promoters are well known in the art and
described, e.g., in Sambrook et al., Molecular Cloning, A
Laboratory Manual (2nd ed. 1989); Kriegler, Gene Transfer and
Expression: A Laboratory Manual (1990); and Current Protocols in
Molecular Biology (Ausubel et al., eds., 1994). Bacterial
expression systems for expressing the zinc finger protein are
available in, e.g., E. coli, Bacillus sp., and Salmonella (Palva et
al., Gene 22:229-235 (1983)). Kits for such expression systems are
commercially available. Eukaryotic expression systems for mammalian
cells, plant cells, yeast, and insect cells are well known in the
art and are also commercially available.
[0096] The promoter used to direct expression of a zinc finger
protein nucleic acid depends on the particular application. Either
a constitutive or an inducible promoter is used, depending on the
particular use of the clone encoding the zinc finger protein.
Exemplary eukaryotic promoters include the CaMV 35 S plant
promoter, SV40 early promoter, SV40 late promoter, metallothionein
promoter, murine mammary tumor virus promoter, Rous sarcoma virus
promoter, polyhedrin promoter, or other promoters shown effective
for expression in eukaryotic cells.
[0097] The promoter typically can also include elements that are
responsive to transactivation, e.g., hypoxia response elements,
Gal4 response elements, lac repressor response element, and small
molecule control systems such as tet-regulated systems and the
RU-486 system (see, e.g., Gossen & Bujard, Proc. Nat'l Acad.
Sci. USA 89:5547 (1992); Oligino et al., Gene Ther. 5:491-496
(1998); Wang et al., Gene Ther. 4:432-441 (1997); Neering et al.,
Blood 88:1147-1155 (1996); and Rendahl et al., Nat. Biotechnol.
16:757-761 (1998)).
[0098] In addition to the promoter, the expression vector typically
contains a transcription unit or expression cassette that contains
all the additional elements required for the expression of the
nucleic acid in host cells, either prokaryotic or eukaryotic. For
example, regulatory elements from eukaryotic viruses are often used
in eukaryotic expression vectors, e.g., SV40 vectors, papilloma
virus vectors, and vectors derived from Epstein-Barr virus.
[0099] A typical expression cassette thus contains a promoter
operably linked, e.g., to the nucleic acid sequence encoding the
zinc finger protein, and signals required, e.g., for efficient
polyadenylation of the transcript, transcriptional termination,
ribosome binding sites, or translation termination. Additional
elements of the cassette may include, e.g., enhancers, and
heterologous spliced intronic signals.
[0100] The particular expression vector used to transport the
genetic information into the cell is selected with regard to the
intended use of the zinc finger protein, e.g., expression in
plants, animals, bacteria, fungus, protozoa, etc. (see, e.g., viral
expression vectors described below and in the Example section).
Standard bacterial expression vectors include plasmids such as
pBR322 based plasmids, pSKF, pET23D, and commercially available
fusion expression systems such as GST and LacZ. A preferred fusion
protein is the maltose binding protein, "MBP." Such fusion proteins
are used for purification of the zinc finger protein. Epitope tags
can also be added to recombinant proteins to provide convenient
methods of isolation, for monitoring expression, and for monitoring
cellular and subcellular localization, e.g., c-myc or FLAG.
[0101] Some expression systems have markers for selection of stably
transfected cell lines such as neomycin, thymidine kinase,
hygromycin B phosphotransferase, and dihydrofolate reductase. High
yield expression systems are also suitable, such as using a
baculovirus vector in insect cells, with a zinc finger protein
encoding sequence under the direction of the polyhedrin promoter or
other strong baculovirus promoters.
[0102] The elements that are typically included in expression
vectors also include a replicon that functions in E. coli, a gene
encoding antibiotic resistance to permit selection of bacteria that
harbor recombinant plasmids, and unique restriction sites in
nonessential regions of the plasmid to allow insertion of
recombinant sequences.
[0103] Standard transduction methods are used to produce bacterial,
mammalian, yeast or insect cell lines that express the zinc finger
proteins of the invention. Transformation of eukaryotic and
prokaryotic cells are performed according to standard techniques
(see, e.g., Morrison, J. Bact. 132:349-351 (1977); Clark-Curtiss
& Curtiss, Methods in Enzymology 101:347-362 (Wu et al., eds,
1983). These methods include the lipofection, microinjection,
ballistics, virosomes, liposomes, immunoliposomes, polycation or
lipid:nucleic acid conjugates, naked DNA, artificial virions,
agent-enhanced uptake of DNA, use of calcium phosphate
transfection, polybrene, protoplast fusion, electroporation,
plasmid vectors, viral vectors, both episomal and integrative, and
any of the other well known methods for introducing cloned genomic
DNA, cDNA, synthetic DNA or other foreign genetic material into a
host cell (see, e.g., Sambrook et al., supra, see also U.S. Pat.
No. 5,049,386, U.S. Pat. No. 4,946,787; U.S. Pat. No. 4,897,355; WO
91/17424, and WO 91/16024). It is only necessary that the
particular genetic engineering procedure used be capable of
successfully introducing at least one gene into the host cell
capable of expressing the protein of choice.
[0104] B. Viral Vectors
[0105] A preferred method of delivering the libraries of the
invention to cells is with viral vector delivery systems, including
DNA and RNA viruses, which have either episomal or integrated
genomes after delivery to the cell. The use of RNA or DNA viral
based systems for the delivery of nucleic acids encoding randomized
zinc finger protein take advantage of highly evolved processes for
targeting a virus to specific cells in the body and trafficking the
viral payload to the nucleus. Conventional viral based systems for
the delivery of zinc finger proteins could include retroviral,
lentiviral, adenoviral, adeno-associated, herpes simplex virus, and
TMV-like viral vectors for gene transfer. Viral vectors are
currently the most efficient and versatile method of gene transfer
in target cells and tissues. Integration in the host genome is
possible with the retrovirus, lentivirus, and adeno-associated
virus gene transfer methods, often resulting in long term
expression of the inserted transgene. Additionally, high
transduction efficiencies have been observed in many different cell
types and target tissues.
[0106] The tropism of a retrovirus can be altered by incorporating
foreign envelope proteins, expanding the potential target
population of target cells. Lentiviral vectors are retroviral
vectors that are able to transduce or infect non-dividing cells and
typically produce high viral titers. Selection of a retroviral gene
transfer system would therefore depend on the target tissue.
Retroviral vectors are comprised of cis-acting long terminal
repeats with packaging capacity for up to 6-10 kb of foreign
sequence. The minimum cis-acting LTRs are sufficient for
replication and packaging of the vectors, which are then used to
integrate the therapeutic gene into the target cell to provide
permanent transgene expression. Widely used retroviral vectors
include those based upon murine leukemia virus (MuLV), gibbon ape
leukemia virus (GaLV), simian immuno-deficiency virus (SUV), human
immuno-deficiency virus (HIV), and combinations thereof (see, e.g.,
Buchscher et al., J. Virol. 66:2731-2739 (1992); Johann et al., J.
Virol. 66:1635-1640 (1992); Sommerfelt et al., Virol. 176:58-59
(1990); Wilson et al., J. Virol. 63:2374-2378 (1989); Miller et
al., J. Virol. 65:2220-2224 (1991); PCT/US94/05700).
[0107] In applications where transient expression of the zinc
finger protein is preferred, adenoviral based systems are typically
used. Adenoviral based vectors are capable of very high
transduction efficiency in many cell types and do not require cell
division. With such vectors, high titer and levels of expression
have been obtained. This vector can be produced in large quantities
in a relatively simple system. Adeno-associated virus ("AAV")
vectors are also used to transduce cells with target nucleic acids
(see, e.g., West et al., Virology 160:38-47 (1987); U.S. Pat. No.
4,797,368; WO 93/24641; Kotin, Human Gene Therapy 5:793-801 (1994);
Muzyczka, J. Clin. Invest. 94:1351 (1994). Construction of
recombinant AAV vectors are described in a number of publications,
including U.S. Pat. No. 5,173,414; Tratschin et al., Mol. Cell.
Biol. 5:3251-3260 (1985); Tratschin et al., Mol. Cell. Biol.
4:2072-2081 (1984); Hermonat & Muzyczka, Proc. Nat'l Acad. Sci.
USA 81:6466-6470 (1984); and Samulski et al., J. Virol.
63:03822-3828 (1989).
[0108] Packaging cells are used to form virus particles that are
capable of infecting a host cell. Such cells include 293 cells,
which package adenovirus, and .psi.2 cells or PA317 cells, which
package retrovirus. Viral vectors used in gene therapy are usually
generated by producer cell line that packages a nucleic acid vector
into a viral particle. The vectors typically contain the minimal
viral sequences required for packaging and subsequent integration
into a host, other viral sequences being replaced by an expression
cassette for the protein to be expressed. The missing viral
functions are supplied in trans by the packaging cell line. For
example, AAV vectors typically only possess ITR sequences from the
AAV genome which are required for packaging and integration into
the host genome. Viral DNA is packaged in a cell line, which
contains a helper plasmid encoding the other AAV genes, namely rep
and cap, but lacking ITR sequences. The cell line is also infected
with adenovirus as a helper. The helper virus promotes replication
of the AAV vector and expression of AAV genes from the helper
plasmid. The helper plasmid is not packaged in significant amounts
due to a lack of ITR sequences. Contamination with adenovirus can
be reduced by, e.g., heat treatment to which adenovirus is more
sensitive than AAV.
[0109] In many situations, it is desirable that the vector be
delivered with a high degree of specificity to a particular cell
type. A viral vector is typically modified to have specificity for
a given cell type by expressing a ligand as a fusion protein with a
viral coat protein on the viruses outer surface. The ligand is
chosen to have affinity for a receptor known to be present on the
cell type of interest. For example, Han et al., Proc. Nat'l Acad.
Sci. USA 92:9747-9751 (1995), reported that Moloney murine leukemia
virus can be modified to express human heregulin fused to gp70, and
the recombinant virus infects certain human breast cancer cells
expressing human epidermal growth factor receptor. This principle
can be extended to other pairs of virus expressing a ligand fusion
protein and target cell expressing a receptor. For example,
filamentous phage can be engineered to display antibody fragments
(e.g., FAB or Fv) having specific binding affinity for virtually
any chosen cellular receptor. Although the above description
applies primarily to viral vectors, the same principles can be
applied to nonviral vectors. Such vectors can be engineered to
contain specific uptake sequences thought to favor uptake by
specific target cells.
Assays for Determining Regulation of Gene Expression by Zinc Finger
Proteins
[0110] A variety of assays can be used to screen for phenotypic
changes upon transduction of cells with the library encoding
randomized zinc finger proteins. A phenotype can be assessed by
measuring, e.g., protein or mRNA levels, product levels, enzyme
activity; transcriptional activation or repression of a reporter
gene; second messenger levels (e.g., cGMP, cAMP, IP3, DAG,
Ca.sup.2+); cytokine and hormone production levels using, e.g.,
immunoassays (e.g., ELISA and immunohistochemical assays with
antibodies), hybridization assays (e.g., RNase protection,
northerns, in situ hybridization, oligonucleotide array studies),
colorimetric assays, amplification assays, enzyme activity assays,
and other phenotypic assays.
[0111] For high throughput applications, typically either cells are
pooled and transduced in a batch, and then individually screened
using flow cytometry, or the cells are pooled into clonal arrays
and screened, e.g., with liquid robotics (see Example section).
Examples of assays for a selected phenotype include e.g., changes
in proliferation, anchorage dependence, growth factor dependence,
foci formation, and growth in soft agar; apoptosis assays, e.g.,
DNA laddering and cell death, expression of genes involved in
apoptosis; signal transduction assays, e.g., changes in
intracellular calcium, cAMP, cGMP, IP3, changes in hormone and
neurotransmitter release; receptor assays, e.g., estrogen receptor
and cell growth; growth factor assays, e.g., EPO, hypoxia and
erythrocyte colony forming units assays; enzyme production assays,
e.g., FAD-2 induced oil desaturation; pathogen resistance assays,
e.g., insect, bacterial, and viral resistance assays; chemical
production assays, e.g., penicillin production; transcription
assays, e.g., reporter gene assays; and protein production assays,
e.g., VEGF ELISAs.
[0112] In one embodiment, the assay for the selected phenotype is
performed in vitro. In one preferred assay format, zinc finger
protein regulation of gene expression in cultured cells is examined
by determining protein production using an ELISA assay or an
immunoassay such as fluorescence activated cell sorting.
[0113] In another embodiment, zinc finger protein regulation of
gene expression is determined by measuring the level of target gene
mRNA expression. The level of gene expression is measured using
amplification, e.g., using PCR, LCR, or hybridization assays, e.g.,
northern hybridization, RNase protection, dot blotting. RNase
protection is used in one embodiment. The level of protein or mRNA
is detected using directly or indirectly labeled detection agents,
e.g., fluorescently or radioactively labeled nucleic acids,
radioactively or enzymatically labeled antibodies, and the like, as
described herein.
[0114] Alternatively, a reporter gene system, e.g., that measures
activation of a gene in a pathway, can be devised using a promoter
operably linked to a reporter gene such as luciferase, green
fluorescent protein, CAT, or .beta.-gal. The reporter construct is
typically co-transfected into a cultured cell. After treatment with
the zinc finger protein of choice, the amount of reporter gene
transcription, translation, or activity is measured according to
standard techniques known to those of skill in the art.
Identification and Isolation of Genes Associated with a Selected
Phenotype
[0115] After assaying for phenotypic changes, as described above,
those cells exhibiting an altered phenotype are selected for
further study, in which the genes associated with the change in
phenotype are identified and isolated. The genes are identified and
isolated, e.g., using differential gene expression analysis with
microarrays; reverse genetics; e.g., identification of genes using
zinc finger proteins to probe YAC or BAC clones and using zinc
finger proteins to scan genomic sequences; subtractive
hybridization; differential cDNA cloning frequencies, subtractive
hybridization; by cloning ESTs from cells of interest; by
identifying genes that are lethal upon knockout; by identifying
genes that are up- or down-regulated in response to a particular
developmental or cellular event or stimuli; by identifying genes
that are up- or down-regulated in certain disease and pathogenic
states; by identifying mutations and RFLPs; by identifying genes
associated with regions of chromosomes known to be involved in
inherited diseases; by identifying genes that are temporally
regulated, e.g., in a pathogenic organism; differences based on
SNPs; by cross-linking the zinc finger protein to the DNA with
which it is associated, followed by immunoprecipitation of the zinc
finger protein and sequencing of the DNA, etc.
[0116] In one embodiment, the candidate genes are identified by
comparing patterns of gene expression associated with the
phenotypic change. For instance, down regulation of a gene by a
ZFP-KRAB will result in under representation of the corresponding
mRNA when compared to a control (i.e., KRAB alone). There are
several methods that can be employed to compare patterns of gene
expression including differential hybridization screening (see,
e.g., Tedder et al., Proc. Nat'l Acad. Sci. USA 85:208-212 (1988)),
subtractive library construction (see, e.g., Davis et al., Nature
308:149-153 (1984)), representational difference analysis (RDA)
(see, e.g., Hubank, Nucleic Acid Res 22:5640-5648 (1994)); Lisitsyn
et al., Science 259:640-648 (1993)) differential display (see,
e.g., Liang et al., Nucleic Acid Res 21:3269-3275 (1993); Liang et
al., Science 257:967-971 (1992)), conventional cDNA array
hybridization (see, e.g., Schummer et al., Biotechniques
23:1087-1092 (1997)) and serial analysis of gene expression (SAGE)
(see, e.g., Velculescu et al., Science 276:1268-1272 (1997)).
[0117] In another embodiment, a technique called suppression
subtractive hybridization (SSH) is used, which is a modification of
the RDA as it normalizes for mRNA abundance (see, e.g, Daitchenko
et al., Proc. Natl. Acad. Sci. USA 93:6025-6030 (1996)). This
technique will be used to compare gene expression profiles of a
target cell pre-and-post zinc finger protein transfection. This SSH
cDNA library may be further screened using microarrays containing
oligonucleotide libraries representing cDNA from relevant tissue
types or, ultimately, oligonucleotides representing all open
reading frames in the entire genome. This combined screening of SSH
cDNA libraries and microchip arrays screening will allow for the
identification of putative functions and pathway relationships for
uncharacterized genes.
[0118] Bacterial artificial chromosomes (BAC) or yeast artificial
chromosomes (YAC) containing large chromosomal segments
representing the entire human genome can be employed to determine
the gene (or genes) responsible for the observed phenotype. YAC or
BAC clones containing the candidate gene can be identified by
physical capture using zinc finger protein or, alternatively, by
probing arrayed clones.
[0119] Direct capture relies on physically binding and separating
clones containing target DNA from the overall population of clones.
Candidate zinc finger proteins are added to BAC or YAC libraries,
using buffer conditions equivalent to those used in biochemical
analysis of zinc finger proteins (see, e.g., U.S. Ser. No.
09/229,037, filed Jan. 12, 1999, and Ser. No. 09/229,007, filed
Jan. 12, 1999). Certain factors should be carefully adjusted so as
to optimize specific binding by the zinc finger protein. Important
chemical factors are zinc and salt (usually either potassium or
sodium chloride). Zinc ion concentration should be 10 micromolar or
less and salt should be 50 millimolar or more. zinc finger protein
and library DNA is added to the buffer. The amounts of each
reactant is important. Highest specificity is obtained when the
zinc finger protein is added at a concentration that is below the
dissociation constant (as judged by gel shifts) of the protein for
its designed target. The reaction is allowed to equilibrate at room
temperature.
[0120] Modifications could include performing the initial binding
at protein concentrations above the dissociation constant in order
to maximize binding. The process could be repeated using only the
retained clones with concentrations of proteins that maximize
specificity (i.e., slightly below the dissociation constant).
Another variation is separating clones into pools rather than
employing the entire library. The number of discrete clones in each
pool would depend on the total library size. For a library size of
1,000,000 clones, ten pools of 100,000 clones or 100 pools of
10,000 clones and so on could be employed. Following equilibration,
the ZFP:DNA complex can be removed from the bulk solution by
affinity capture of the zinc finger protein. Potential ligands are
FLAG, MBP, biotin, 6.times. His or any other tag for which an
acceptable receptor exists. The receptor should be immobilized to
an inert support such as magnetic beads or sepharose resin.
Appropriate receptors would be FLAG antibody (FLAG epitope),
amylose (MBP), streptavidin (biotin), nickel (6.times.His).
[0121] Once the clones are identified by capture they are sequenced
to identify coding regions. The genomic inserts cloned into the
BACs or YACs may be too large to pinpoint the exact gene
responsible for the phenotype. The list of possible candidate genes
within a clone could be narrowed in the cases where clones with
overlapping, but not identical, sequences were captured. Only the
regions common to both clones should contain the candidate genes.
Alternatively, clones containing smaller segments of each BAC and
YAC could then be used for capture. Other vectors used could
include lamba, P1 or cosmids.
[0122] In another embodiment, physical capture and retention of DNA
in solution is an array-based method where zinc finger proteins are
used as probes to detect clones possessing the correct target
sequences. BAC and YAC libraries would be arrayed so that each
clone would occupy an unique address on a support such as glass,
nitrocellulose or any other material which allows nondestructive
immobilization of DNA. The zinc finger proteins would be conjugated
to a fluorophore either pre- or posttranslationally. The supports
containing the clones would be flooded with the zinc finger protein
and incubated for a sufficient time to allow binding. Then unbound
zinc finger protein would be washed off using conditions that
minimize non-specific binding. Binding would be visualized by
exposing the filter to an appropriate wavelength of light, exciting
the fluorophore to emit at a characteristic wavelength.
[0123] This method could be refined by simultaneously adding two
zinc finger proteins labeled with different fluorophores. By using
the fluorophores emitting at appropriate wavelengths, binding of
both zinc finger proteins to the same clone could be detected
simultaneously by monitoring the output color which should be a
combination of both wavelengths. For instance, the presence of a
blue emitting fluorophore and a yellow emitting fluorophore would
produce green light. Flurophores could be fluorescent proteins that
are modified from green fluorescent protein to produce the spectrum
of wavelengths. Alternatives would be fluorescent dyes with
reactive groups that can be conjugated to protein moieties post
purification or fluorescently labeled antibodies. As with physical
capture, once a BAC or YAC clone is identified different regions
can be probed in sublibraries with shorter inserts.
[0124] In another embodiment, physical capture is achieved by
tagging DNA targets which are bound by their specific zinc finger
protein by using a modified catalyzed reporter deposition (CARD)
method. CARD has been used as a means of signal amplification in
immunocytochemistry, ELISA, and blotting (Adams, J. Histochem.
Cytochem. 40:1457-1463 (1992); Bobrow et al., J. Immunol. Meth.
150:145-149 (1992)). This technique normally involves the use of
horseradish peroxidase (HRP) in the presence of hydrogen peroxide
to catalyze biotinylated tyramine deposition around the site of the
enzyme activity. This results in biotinylation of molecules or
motifs that are proximal to the active enzyme. This technique has
been adapted to allow the specific recovery of neighboring phage
antibodies binding around a core ligand binding site on a cell
surface (Osbourn et al., Nat. Biotech. 16:778-781 (1998)).
[0125] A modified CARD technique would be used to biotinylate genes
which have been recognized by the randomized zinc finger proteins
of the invention. The zinc finger proteins could be either directly
engineered as HRP fusions, or HRP conjugated antibodies which
recognize the zinc finger proteins (by their FLAG sequence for
instance) could be used. HRP conjugated anti-FLAG monoclonal
antibody and biotin tyramine are added to an equilibrated solution
of zinc finger proteins and libraries. In either scenario, the
biotin which is covalently attached to DNA sequences surrounding
the zinc finger proteins, and this biotin "tag" will provide a
handle for further manipulation. The biotinylated DNAs can be
captured and purified with streptavidin-coated magnetic beads
(Dynal, Oslo). Another way to capture the DNAs that are recognized
by zinc finger proteins is to use an anti-FLAG affinity column to
purify the DNAs. Post capture the DNAs will be cloned, sequenced
and otherwise characterized.
[0126] In another embodiment, genes are identified by scanning
genomic sequences. The target gene sequence can be predicted based
on the recognition residues of each zinc finger. By using these
rules for amino acid side-chain contacts with nucleotide bases, the
nucleotide sequence can be "read off" of the zinc finger protein.
Allowances for ambiguities can be made based on a knowledge of
specificity for each interaction or combinations of interactions.
Genes can be identified by searching the Genbank DNA database
(National Center of Biotechnology Information) for matching
sequences using an algorithm such as BLAST (Altschul et al., J.
Mol. Biol. 215:403-410 (1990)). Ultimately it will be possible to
search the whole human genome. The expectation is that many of the
zinc finger proteins targeting candidate genes will recognize
different sequences of the same gene or genes. Thus, confirmation
that any one zinc finger protein is truly targeting a particular
gene is obtained by grouping the genes identified by different zinc
finger proteins and deriving a consensus.
[0127] In another embodiment, genes are identified by cross linking
the zinc finger protein and the nucleic acid (or chromatin) to
which it is bound, immunoprecipitating the cross-linked complex,
and then sequencing the nucleic acid to identify the gene of
interest. Sequence-specific binding of a zinc finger protein to
chromatin is assayed, e.g., by chromatin immunoprecipitation
(ChIP). Briefly, this technique involves the use of a specific
antibody to immunoprecipitate chromatin complexes comprising the
corresponding zinc finger protein antigen, and examination of the
nucleotide sequences present in the immunoprecipitate.
Immunoprecipitation of a particular sequence by the antibody is
indicative of interaction of the zinc finger protein antigen with
that sequence (see, e.g., O'Neill et al. in Methods in Enzymology,
Vol. 274, pp. 189-197 (1999); Kuo et al., Method 19:425-433 (1999);
and Ausubel et al., supra, Chapter 21).
[0128] In one embodiment, the chromatin immunoprecipitation
technique is applied as follows. The zinc finger protein is
introduced into a cell and, after a period of time sufficient for
binding of the zinc finger protein to its binding site has elapsed,
cells are treated with an agent that crosslinks the zinc finger
protein to chromatin if that molecule is stably bound. The zinc
finger protein can be crosslinked to chromatin by, for example,
formaldehyde treatment or ultraviolet irradiation. Subsequent to
crosslinking, cellular nucleic acid is isolated, sheared and
incubated in the presence of an antibody directed against the zinc
finger protein. Antibody-antigen complexes are precipitated,
crosslinks are reversed (for example, formaldehyde-induced
DNA-protein crosslinks can be reversed by heating) and the sequence
content of the immunoprecipitated DNA is tested for the presence of
a specific sequence, for example, the target site of the zinc
finger protein.
[0129] In a preferred embodiment, the immunoprecipitated DNA is
tested for the presence of specific sequences by a sensitive
hydrolyzable probe assay allowing real-time detection of an
amplification product, known colloquially as the Taqman.RTM. assay.
See U.S. Pat. No. 5,210,015; Livak et al., PCR Meth. App. 4:357-362
(1995); and Heid et al., Genome Res. 6:986-994 (1995). Briefly, an
amplification reaction (e.g., PCR) is conducted using a probe
designed to hybridize to a target sequence flanked by two
amplification primers. The probe is labeled with a fluorophore and
a fluorescence quencher such that, when not hybridized to its
target sequence, the probe does not emit detectable fluorescence.
Upon hybridization of the probe to its target and hydrolysis of the
probe by the polymerase used for amplification, the fluorophore is
released from the vicinity of the quencher, and fluorescence
increases in proportion to the concentration of amplification
product. In this assay, the presence of increased levels of an
amplification product corresponding to the binding site for the
zinc finger protein, compared to levels of amplification product
specific to a control genomic sequence, is indicative of binding of
the zinc finger protein to its binding site in cellular
chromatin.
[0130] Additional methods for detecting binding of zinc finger
protein to chromatin include, but are not limited to, microscopy
(e.g., scanning probe microscopy), fluorescence in situ
hybridization (FISH) and fusion of a DNA methylase domain to the
zinc finger protein, in which case sequences to which the zinc
finger protein is bound become methylated and can be identified,
for example, by comparing their sensitivity to
methylation-sensitive and methylation-dependent restriction enzymes
or by using antibodies to methylated DNA. See, for example, van
Steensel et al., supra.
[0131] All publications and patent applications cited in this
specification are herein incorporated by reference as if each
individual publication or patent application were specifically and
individually indicated to be incorporated by reference.
[0132] Although the foregoing invention has been described in some
detail by way of illustration and example for purposes of clarity
of understanding, it will be readily apparent to one of ordinary
skill in the art in light of the teachings of this invention that
certain changes and modifications may be made thereto without
departing from the spirit or scope of the appended claims.
EXAMPLES
[0133] The following examples are provided by way of illustration
only and not by way of limitation. Those of skill in the art will
readily recognize a variety of noncritical parameters that could be
changed or modified to yield essentially similar results.
Example 1
Protocol for Preparation and Screening Using a Randomized Zinc
Finger Protein Library Generated by Finger Grating
[0134] A. Generation of a Library Using Finger Grafting
[0135] A library of 12 different helices compatible with 5
different finger positions will be created and assembled into zinc
finger proteins using a method similar to that currently used to
assemble engineered 3 finger proteins (see, e.g., U.S. Ser. No.
09/229,037 filed Jan. 12, 1999, and U.S. Ser. No. 09/229,007, filed
Jan. 12, 1999). Randomness will be confirmed by sequencing a
representative sample. Of this library, 250,000 individual
bacterial transformants will be picked and archived. The individual
transformants will be combined into pools of 8 and cloned into a
viral delivery vector (such as an adenoviral vector).
[0136] Viral delivery particles will be produced from each pool
(there are 31,250 different pools) and tested in an appropriate
assay for the identification of a desired phenotype. Assays could
be the development of growth factor independence, secretion of EPO,
angiogenesis, apoptosis etc.
[0137] Biologically active zinc finger proteins ("hits") will be
confirmed by secondary screening. The gene directly responsible for
the phenotype will be identified either by virtue of proximity of a
binding site (the binding site for the active zinc finger protein
can be surmised by helix composition or determined experimentally
by site selection) if the sequence is known, or pulled from a
genomic library using the zinc finger protein itself as a molecular
probe.
[0138] Specific recognition helices for twelve different DNA
triplet sequences have been characterized. These helices are
referred to by their "SBS" numbers. The table below shows the
target DNA triplet sequences and the amino acid composition of the
twelve different recognition helices. Any 5-finger zinc finger
protein comprising a unique subset of 5 of these 12 recognition
helices will recognize a distinct and unique 15 basepair DNA
sequence. TABLE-US-00001 TABLE 1 SBS Number Target Triplet
Recognition Helix SBS1 GTG RSDALTR SBS2 GAG RSDNLAR SBS3 GGG
RSDHLSR SBS4 GCG RSDELTR SBS5 GCA QSGSLTR SBS6 GCT QSSDLTR SBS7 GCC
ERGTLAR SBS8 GAT QSSNLAR SBS9 GAC DRSNLTR SBS10 GAA QSGNLAR SBS11
GGC DRSHLAR SBS12 GGA QSGHLQR
[0139] For example, a zinc finger protein made up of (reading from
the C-terminus) SBS 4-10-9-10-2 would recognize the DNA sequence
(reading 5' to 3') GCG GAA GAC GAA GAG.
[0140] DNA encoding each of the 12 SBS helices are synthesized as
oligonucleotides. These oligonucleotides are mixed in equimolar
amounts and combined with oligonucleotides that encode the
remaining amino acids of a five finger zinc finger protein based on
the amino acid sequence of the murine zinc finger protein Zif268.
This mixture is PCR amplified as described in U.S. Ser. No.
09/229,037, filed Jan. 12, 1999, and U.S. Ser. No. 09/229,007,
filed Jan. 12, 1999, and subcloned into two different mammalian
expression such that one vector produces a chimeric transcription
factor comprising a nuclear localization sequence, the zinc finger
protein DNA binding domain, the VP16 activating domain and the FLAG
epitope tag (this vector is referred to using an acronym of its
component parts; NVF). The second vector is identical to the first
except that a KRAB transcriptional repression domain replaces the
VP16 domain (NKF). The rest of the vector sequences support the
production of virus-based delivery components such as the sequences
required for recombination into adenoviral vectors and packaging
into viral particles
[0141] These vectors are used to transform E. coli. Individual
colonies, representing distinct individual zinc finger protein
clones, are picked and subcultured in 96-well microtiter dishes.
250,000 clones are picked and arrayed for each vector system (NVF
and NKF). This creates an arrayed zinc finger protein library
comprising approximately 2,600 microtiter plates. These libraries
are stored as glycerol stocks at -80.degree. C.
[0142] DNA sequence analysis of a subset of each library confirms
that the five finger zinc finger proteins encoded represent a
random assortment of the 12 recognition helices.
[0143] The zinc finger protein E. coli clone library is converted
to a pooled viral delivery library as follows. The E. coli clones
are arranged into pools of 8 different clones by pipetting adjacent
wells together using a 12-channel multi-channel pipette (this can
be done robotically). The pools are grown in rich medium using
deep-well microtiter dishes at 37.degree. C. Plasmid DNA is
prepared using Qiagen columns. The DNA pools are then used to
transfect PERC.6 cells (a cell line used to produce adenoviral
vectors). Several days later the viral vector-laden culture
supernatants are collected and stored at -80.degree. C.
[0144] B. Screening for the Selected Phenotype
[0145] An assay for a particular desired phenotype is now created
and implemented using a microtiter-based method. The viability of a
growth factor dependent cell line, capable of detecting autocrine
production of a growth factor such as EPO or VEGF is one such
assay, described below.
[0146] Once the assay is created, the influence of the zinc finger
protein library members on the assay can be determined using
robotic methods commonly employed in the high throughput screening
industry. A sample, in this case a pool of 8 different zinc finger
proteins carried in adenoviral delivery vectors, is added to a well
of an assay plate, in this case a growth factor dependent cell line
in minimal medium. Several days later the assay plate is tested to
determine if any of the zinc finger proteins caused the cell line
to grow. Growth can be determined using many different high
throughput assays, in this case by the metabolic conversion of a
fluorescent dye Alamar Blue.
[0147] Hits from the high throughput assay (wells where cell growth
was supported) are confirmed by simply retesting the pool and then
the pool is "deconvoluted," separating it into individual zinc
finger protein component members and retested to determine which of
the 8 zinc finger proteins triggered cell growth.
[0148] Once a zinc finger protein-phenotype connection has been
established, mechanistic and genomic analyses can be performed to
identify the gene responsible for the phenotype. In this case, the
independence of growth factors suggests autocrine production of a
growth factor. This can be simply confirmed by testing the growth
supporting nature of zinc finger protein treated conditioned medium
on otherwise untreated growth factor dependent cells.
[0149] After the autocrine mechanism has been confirmed, the task
becomes one of determining which growth factor gene was switched on
by the zinc finger protein library member. Well characterized
growth factors can be eliminated by using inactivating antibodies.
Suspect genes can be identified by scanning the sequence databases
for the 15 basepairs recognized by the active zinc finger protein.
This sequence can be determined either by simply reading off the
recognition helices' amino acid sequence and predicting the DNA
target sequence using the relationships outlined in the table
above, or by using site selection experiments as described in
previous applications to determine the DNA target sequence
empirically. Suspect (or candidate) genes can also be identified
using experimental method designed to measure global differential
gene expression (such as gene expression microarrays). Finally, the
zinc finger protein itself can be used as a probe for YAC or BAC
clones to identify candidate loci.
[0150] C. Screening Using Flow Cytometry
[0151] In addition to screening using microtiter type assays (as
described above), flow cytometry and cell sorting can be used to
screen for specific phenotypes. A flow cytometer simply measures
the fluorescence of one cell at a time as a stream of cells flow
past a laser. Multiple lasers and multiple detectors permit
simultaneous detection of several fluorophores (typically up to 4).
A wide variety of fluorescent probes have been developed allowing
the measurement of cell surface markers, DNA content, green
fluorescent protein and other cytoplasmic components. Multi-marker
analysis allows one to study a specific cell population, defined by
specific cell surface markers, in complex mixtures of cells such as
whole blood. In addition to simply detecting the kind and intensity
of specific fluorescent markers on cells as they flow past the
laser beams, cytometers with sorting capability can collect
specific populations of cells one cell at a time. This permits the
outgrowth of very specific cell populations (if the labeling method
is not toxic, not always the case) and/or the application of
bulk-type assays (western blots, northern blots etc.) on
homogeneous and very specific populations of cells.
[0152] In screening, a cell sorter permits the isolation of a
single cell or a population of cells displaying a desired
phenotype. This could be the appearance of a specific receptor on
the surface of a cell treated by a specific cytokine (i.e. the
appearance of ICAM on the surface of cells treated with IL-1) or
any other measurable response.
[0153] In practice, a library is created in retroviral vectors.
This could be the same zinc finger protein library described above.
Susceptible cells (for example U937 monocytic cells) are transduced
using the retroviruses. A specific phenotype is detected using flow
cytometry and cells displaying the desired phenotype collected into
separate wells of a microtiter plate. The zinc finger proteins
causing the desired phenotype can then identified by rescuing the
retroviral sequences using PCR.
Example 2
Protocol for Preparation and Screening Using a Randomized Zinc
Finger Protein Library Generated by Codon Doping
[0154] A. Preparation of the Library Using Codon Doping
[0155] As described above, each zinc finger binds three nucleotides
using four critical amino acids in the recognition helix. If each
base in the codons for these amino acids was simply randomized, it
would generate a library of 4.sup.12 clones (1.7 million). This
number is already in excess of a desired library limit of about one
million to about 10 or 100 million clones and only concerns one
finger (and three are to be used in these methods). However, it is
not necessary to use completely random codons. Because of the
redundancy of the genetic code, schemes of semi-randomization can
generate representatives of all, or nearly all codons. This
strategy is thus called a codon doping scheme.
[0156] One randomization scheme uses VNS instead of NNN, where
N=any base, V=A or G and S=G or C. All of the codons are
represented by VNS except Phe, Trp, Tyr, Cys and all translation
termination codons. It is advantageous to eliminate the termination
codons and loss of the four amino acids listed is tolerable because
they are typically underrepresented in known protein DNA contacts.
With the VNS scheme it is possible to randomize 4 amino acids in
significantly less than a million clones (331,776 to be exact).
However, varying a fifth position pushes the library size into the
8 million clone range. Some finger positions will still need to be
fixed. The four critical amino acids of finger 1 will be randomized
using the VNS scheme and fingers 2 and 3 will be fixed to recognize
the DNA sequence GGG GAG. Specific fingers for these triplets are
available that do not recognize alternative binding sites. This 6
base pair anchoring sequence will occur once every 46 (4096) bases
and should lie within a reasonable distance of the transcription
initiation site of most genes. The randomized finger will direct
the zinc finger proteins to subsets of these anchoring sites with 3
or 4 additional bases of sequence specificity. In future
experiments additional libraries can be examined that carry
alternative anchoring fingers.
[0157] The mutagenesis strategies proposed to generate the
three-finger zinc finger protein library is represented below:
TABLE-US-00002 TABLE 2 -1 1 2 3 4 5 6 Finger 1 VNS S VNS VNS L A
VNS Finger 2 R S D N L A R Finger 3 R S D H L S R
[0158] To balance the diversity and size of the zinc finger protein
library, the relatively highly conserved serine is fixed at
position 1; leucine at position 4 (which does not contact DNA but
is involved in stabilizing the fold of the finger); and a small
alanine at position 5. All the randomization will be built in by
polymerase chain reaction using 3 degenerate oligos (2, 4, and 6)
that contain the VNS dope schemes for the -1, 2, 3, and 6 positions
(FIG. 1).
[0159] Codon Doping Protocol
[0160] 1. Dilute the following oligos to 0.5 .mu.M in H.sub.2O
[0161] Oligo 1: SCOM (Sangamo Common Oligo) 1
[0162] Oligo 2: Oligo encoding randomized finger 1:
(VNS)S(VNS)(VNS)LA(VNS), see text for explanation of notation.
[0163] Oligo 3: SCOM 2
[0164] Oligo 4: Oligo encoding finger 2: RSDNLAR
[0165] Oligo 5: SCOM 3
[0166] Oligo 6: Oligo encoding finger 3: RSDHLSR
[0167] 2. Set up PCR reactions as follows: TABLE-US-00003 50 .mu.l
2X PCR Master Mix, Boehringer Mannheim 1 .mu.l SCOM 1 1 .mu.l SCOM
2 1 .mu.l SCOM 3 1 .mu.l Randomized Finger 1 oligo 1 .mu.l Finger 2
oligo 1 .mu.l Finger 3 oligo 44 .mu.l H.sub.2O 100 .mu.l total
volume
[0168] 3. Run the following PCR program to form the initial
"scaffold" of oligos (see diagram):
[0169] 95.degree. C. 5 minutes; 95.degree. C. 30 seconds;
40.degree. C. 30 seconds.times.4 cycles
[0170] 72.degree. C. 1 minute
[0171] 4. Then add external primers (SCOM F, at 10 .mu.M, and SCOM
R, at 10 .mu.M), 2 .mu.l of each primer (refer to diagram).
[0172] 5. Continue with the following PCR program:
[0173] 95.degree. C. 1 minute; 95.degree. C. 30 second; 62.degree.
C. 30 seconds.times.30 cycles
[0174] 72.degree. C. 1 minute
[0175] 72.degree. C. 10 minutes
[0176] 4.degree. C. soak
[0177] 6. Run entire reaction through Qiagen PCR Clean-up column.
Elute in 50 .mu.l H.sub.2O
[0178] 7. Set up Kpn I/Bam HI restriction digest: TABLE-US-00004 50
.mu.l clean PCR product 10 .mu.l NEB Bam HI Buffer, 10X 10 .mu.l
NEB BSA, 10X 3 .mu.l NEB Kpn I restriction enzyme, u/l 2 .mu.l NEB
Bam HI restriction enzyme, u/l 25 .mu.l H.sub.2O 100 .mu.l total
volume, incubate at 37.degree. C. for 4 hours.
[0179] 8. Run entire digest on a 1.4% agarose gel (split sample
into two lanes). Gel extract and purify the 300 bp fragment from
each lane using Qiagen Gel Extraction Kit. Elute each in 30 .mu.l
H.sub.2O, then combine for total volume of 60 .mu.l.
[0180] 9. Ligate into a phage vector such as SurfZAP (Stratagene)
that has been modified to possess Kpn I and Bam HI restriction
sites in the appropriate frame as to generate a plasmid encoding
ZFP-cpIII fusion protein.
[0181] 10. Transform into XL-1 Blue bacteria and plate onto LB+100
g/ml ampicillin. Grow overnight at 37.degree. C.
[0182] 11. Pick individual colonies and sequence to ensure that
finger 1 randomization is sufficiently represented.
[0183] B. Packaging Into Viral Vectors for Delivery
[0184] This step entails cloning the zinc finger protein libraries
from the donor phage vectors into an AAV (adeno-associated viral)
vector. Each vector will retain an intact cis-acting ITR sequence,
followed by a cytomegalovirus promoter. The ITR sequences are
required in cis to provide functional origins of replication (ori)
as well as the signals for encapsidation, integration into the cell
genome and rescue from either host cell chromosomes or recombinant
plasmids. To maintain an optimal wild-type AAV genome size for the
vectors, an additional, functionally inert intron sequence will be
incorporated into the DNA construct. This intron will be spliced
out in the final mRNA that would encode the functional zinc finger
protein. The zinc-finger genes will be modified to incorporate a
Kozak sequence for proper translation initiation and add a nuclear
localization sequence such as that from the SV40 T antigen. The
sequence for the assembled zinc finger protein expression
constructs will be as follows: Kozak sequence-NLS-ZFPs-KRAB/VP
16-FLAG.
[0185] Two distinguishable phases of the AAV life cycle can occur
in permissive or non-permissive conditions (see FIG. 3). In
permissive cells, the presence of a helper virus, typically
adenovirus, causes an infecting AAV genome to be greatly amplified
generating a large burst of infectious progeny. This biological
property will be exploited to generate AAV-ZFP vectors at genomic
scale as well as to rescue inserts from relevant target cells if
needed.
[0186] In a productive infection, the infecting parental AAV single
strand genome is converted to a parental duplex replicating form
(RF) by a self-priming mechanism which takes advantage of the
ability of the ITR to form a hairpin structure. This process can
occur in the absence of helper virus but is enhanced by a helper
virus. The parental RF molecule is then amplified to form a large
pool of progeny RF molecules in a process which requires both the
helper functions and the AAV rep gene products, Rep78 and Rep68.
AAV RF genomes are precursors to progeny single strand (SS) DNA
genomes that are packaged into pre-formed empty AAV capsids
composed of VP1, VP2 and VP3 proteins.
[0187] In the absence of a helper virus the AAV genomes reach the
cell nucleus but bulk replication generally does not occur. The
infecting genomes are converted to double stranded DNA (dsDNA) and
may persist as free unintegrated genomes for a considerable number
of cell passages. Expression of exogenous vector genes can occur
from these dsDNA forms and these vector sequences can be rescued
through packaging into new viral particles. These new viral
particles are generated by induction of cell permissiveness through
infection with a helper viruses or transfection with plasmids which
express all of the appropriate helper functions. This biological
characteristic allows rAAV particle recovery by amplification from
a target cell. Therefore, subsequent isolation and characterization
of viruses expressing desired sequences is accomplished in a rapid
and facile manner.
[0188] Protocol for Generating rAAV-ZFP Library
[0189] 1. Isolate "library" of zinc finger protein inserts from
zinc finger protein phage library DNA prep by digesting with Kpn I
and Bam HI restriction enzymes.
[0190] 2. Ligate the Kpn I/Bam HI ZFP-encoding fragment into the
above mentioned AAV vectors. Each AAV vector has already been
modified to possess the NLS-Kpn I site-Bam HI site-VP16 or
KRAB-FLAG. Thereby, the resulting ligations should result in
plasmids encoding NLS-ZFPs-KRAB or VP 16-FLAG.
[0191] 3. Starting with the repressor library (KRAB), transform the
AAV-ZFP-KRAB plasmids into XL-1 Blue bacteria. Grow overnight on
plates.
[0192] 4. Pick resulting colonies and array into 96-well format for
small-scale bacterial cultures (refer to step 1, FIG. 4).
[0193] 5. Isolate AAV-ZFP-KRAB plasmids, maintaining the 96-well
arrayed format.
[0194] 6. Plate 293 cells, already stably expressing the AAV rep
and cap gene products, in 96-well format. This should be done the
day prior to transfection.
[0195] 7. Infect 293 cells with Adenovirus (Ad), incubate for 1
hour at 37.degree. C.
[0196] 8. Using the DEAE-Dextran transfection technique,
cotransfect the AAV-ZFP-KRAB plasmids along with helper plasmid
encoding for the AAV rep and cap gene products. Add the
DNA-DEAE-Dextran solution directly to the infected cells. Incubate
for 4-5 hours at 37.degree. C. Wash cells and replenish with
complete media. Incubate for 72 hours.
[0197] 9. To recover the resulting AAV-ZFP-KRAB viruses
(rAAV-ZFP-KRAB), harvest and lyse the cells (see, e.g., Matsushita
et al., Gene Therapy 5:938-945 (1998)). Clear the lysate of
cellular debris by a low speed centrifugation spin and heat
inactivate the Ad virus. The arrays of rAAV-ZFP libraries can be
stored at -70.degree. C. until assayed.
[0198] C. Selecting a Phenotype of Interest
[0199] The cells transfected with rAAV-ZFPs will each be expressing
different genes in different levels, resulting in different
phenotypes. Generally, one is interested in a specific phenotype
that can be identified easily in a high throughput (HTP) assay.
Numerous assays have been developed which can identify changes in
cell growth and metabolism. The assay employed depends on the
pathway of interest. Once a cell expressing the desired phenotype
is identified, the genes expressed/repressed can be determined.
[0200] Assay for Target Discovery Using Inhibition of VEGF
Induction During Hypoxia
[0201] Vascular Endothelial Growth Factor (VEGF) is the principle
pro-angiogenic factor responsible for eliciting the growth of new
blood supply to hypoxic tissues. VEGF expression is triggered by
hypoxia in a wide variety of cell types. This regulation occurs
principally at the level of transcription. The hypoxic triggering
of VEGF gene expression is central to several important pathologies
both in a negative and positive sense. Blockade of VEGF induction
could lead to the treatment of solid tumor growth and diabetic
retinopathy. Thus, in this example, factors that inhibit hypoxic
stimulation of VEGF are identified.
[0202] The human embryonic kidney epithelium-derived cell line 293
can be induced to secrete VEGF into the growth medium by making the
cells hypoxic or by mimicking hypoxia using cobalt chloride. This
induction can be followed using a simple ELISA.
[0203] 293 cells, previously stably transfected with a gene
expressing secreted alkaline phosphatase (SEAP), will be plated in
a 96-well format. The cultures will be transduced with the
rAAV-ZFP-KRAB library, already arrayed in 96-well format (see
above), and allowed to incubate for 48 hours. Next, VEGF expression
will be induced using CoCl.sub.2. 24 hours post VEGF-induction,
culture supernatants will be tested for VEGF secretion. In
addition, the secretion of SEAP will be examined as a general
control for toxicity and secretion function. Cells that fail to
induce VEGF expression will be scored as primary hits.
[0204] The zinc finger proteins responsible for the primary hits
will be recovered and retested in secondary assays confirming the
specific blockade of the VEGF inducing hypoxic signal (Target
Validation). In this case, a HTP ELISA is employed to identify the
desired phenotypic response in the presence of a specific AAV-ZFP
that has targeted a gene involved in hypoxic stimulation of
VEGF.
[0205] Assay for Target Discovery Using Up-Regulation of E-Cadherin
on the Cell Surface
[0206] E-cadherin is a focal point in the development of numerous
cancers and its function is frequently inactivated in the
development of breast, colon, prostate, stomach, liver, esophagus,
skin, kidney and lung cancers amongst others. The loss of
E-cadherin function is a rate limiting step in the transition of
cells from well differentiated adenoma to invasive carcinoma cells.
Chromatin rearrangement, mutation, hypermethylation, and loss of
transcription-factor binding are all thought to play roles in
suppression of E-cadherin function. Furthermore, alterations in
function, expression levels, and signaling properties of molecules
which associate with E-cadherin have also been shown to play a role
in this loss of function. This widespread loss of function in
numerous cancer types implies a profound role for E-cadherin in
these cancers where it is manifested by de-differentiation,
increased infiltrative growth and metastatic potential.
Re-establishment of E-cadherin function in various cell culture and
in vivo systems has demonstrated the reversion of invasive tumors
to a benign, epithelial phenotype. Therefore, in this example,
genes which could be invoked to up-regulate E-cadherin expression
are identified.
[0207] The cell line which will be selected for use in the
phenotypic screening assay must be able to express E-cadherin at
its surface upon induction of expression by the specifically
constructed zinc finger motif. In this case, the HT-29 human colon
carcinoma cell line, which has been shown to upregulate E-cadherin
expression in response to dimethylsulfoxide (DMSO) in a dose
dependent manner, would be appropriate.
[0208] Once again, cells are plated in a 96-well format. This time,
the cells are transduced with members of a rAA-ZFP-VP 16 library,
produced as described above. They will be examined for the presence
of cell-surface expression of E-cadherin 48 hours
post-transduction. Treatment of the cells with DMSO would serve as
a positive control.
[0209] Determining cell-surface E-cadherin expression can be done
by one of several methods. One method is accomplished by binding
fluorescently tagged antibodies directed against the E-cadherin on
the cell surface. Quantitation of this fluorescence is then
determined by a 96-well fluorometer. Alternatively, a relatively
less sensitive immunohistochemical assay performed in a 96 well
format may be sufficient for evaluation of up-regulation,
supporting the premise of this approach. Another approach to
assaying the upregulation of E-cadherin is based on proteolytic
digestion of a fluorescence labeled protein substrate. This assay
has the potential of being simpler and more sensitive than the one
based on using antibodies to detect E-Cadherin expression. It has
been shown that in some cancer cell types secreted matrix
metalloproteinases are down regulated by the upregulation or
reconstitution of E-cadherin expression. In the proposed high
throughput assay system, a fluorescently tagged protein substrate
(Molecular Probes, EnzChek Assay Kit) does not fluoresce because of
the quenching phenomena observed when numerous fluorescent tags are
in close proximity to one another. However, when this labeled
protein substrate is cleaved by proteases, a fluorescent signal is
observed which corresponds to the proteolytic activity in the
sample. For screening purposes positive hits would be counted where
fluorescence emission is quenched indicating down regulation of
protease activity. These positive samples would then be further
analyzed and tested for E-cadherin expression.
[0210] The up-regulation of E-cadherin would represent the
generation of the desired change in tumor cell phenotype induced by
the zinc finger protein's action on a gene(s) expression. Thus,
indicating that this gene(s) may prove to be a good candidate for
drug discovery.
[0211] D. Identifying Candidate Genes Associated with a Selected
Phenotype
[0212] Once a "hit" has been identified, using, e.g., one of the
assays described above, one must then determine the gene(s) the
zinc finger protein has influenced that resulted in the desired
phenotype. The first step is to identify the zinc finger protein
that was involved. This is easily accomplished as indicated in the
previous section referring to rAAV recovery. By infecting the cells
containing the AAV-ZFP of interest with helper virus, the AAV will
enter a lytic cycle and thereby produce progeny virus. Isolation of
these rAAV particles from the target cell can be done as previously
described. This assures that there is plenty of the rAAV-ZFP for
additional experiments and manipulations. Analysis of the zinc
finger protein can suggest a putative recognition target site that
when compared to sequences listed in GenBank could identify genes
that may be affected by the zinc finger protein.
[0213] Comparing mRNA of ZFP-transduced vs. non-transduced cells is
a direct way of identifying differentially expressed genes. Several
methods have been developed to do this sort of analysis:
subtractive hybridization, differential display and array analysis,
as described above.
* * * * *